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https://www.readbyqxmd.com/read/28319170/antibody-response-against-phop-efficiently-discriminates-among-healthy-individuals-tuberculosis-patients-and-their-contacts
#1
Aurobind Vidyarthi, Nargis Khan, Tapan Agnihotri, Kaneez F Siddiqui, Girish R Nair, Ashish Arora, Ashok K Janmeja, Javed N Agrewala
Tuberculosis continues to be one of the most devastating global health problem. Its diagnosis will benefit in timely initiation of the treatment, cure and therefore reduction in the transmission of the disease. Tests are available, but none can be comprehensively relied on for its diagnosis; especially in TB-endemic zones. PhoP is a key player in Mycobacterium tuberculosis virulence but nothing has been known about its role in the diagnosis of TB. We monitored the presence of anti-PhoP antibodies in the healthy, patients and their contacts...
2017: PloS One
https://www.readbyqxmd.com/read/28316835/clinical-characteristics-of-patients-with-newly-diagnosed-diabetic-macular-edema-in-turkey-a-real-life-registry-study-turk-dem
#2
Bora Eldem, Sengul Ozdek, Ali Osman Saatci, Emin Ozmert, Esat Ulay, Gulsah Nomak
Purpose. To evaluate the clinical and diagnostic characteristics of patients with newly diagnosed diabetic macular edema (DME) in Turkey in a real-life setting. Methods. A total of 945 consecutive patients (mean (SD) age: 61.3 (9.9) years, 55.2% male) with newly diagnosed DME were included. Data on patient demographics, comorbidities, ocular history, ophthalmic examination findings including type of DME, central macular thickness (CMT) via time domain (TD) and spectral domain (SD) optical coherence tomography (OCT), and planned treatments were recorded...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28314076/lactococcus-lactis-carrying-a-dna-vaccine-coding-for-the-esat-6-antigen-increases-il-17-cytokine-secretion-and-boosts-the-bcg-vaccine-immune-response
#3
Vanessa Bastos Pereira, Vanessa Pecini da Cunha, Tatiane Melo Preisser, Bianca Mendes Souza, Meritxell Zurita Turk, Camila Prósperi De Castro, Marcela Santiago Pacheco Azevedo, Anderson Miyoshi
AIMS: A regimen utilizing BCG and another vaccine system as a booster may represent a promising strategy for the development of an efficient tuberculosis vaccine for adults. In a previous work, we confirmed the ability of Lactococcus lactis FnBPA+ (pValac:ESAT-6), a live mucosal DNA vaccine, to produce a specific immune response in mice after oral immunization. In the present study, we examined the immunogenicity of this strain as a booster for the BCG vaccine in mice. METHODS AND RESULTS: After immunization, cytokine and immunoglobulin profiles were measured...
March 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28301284/immunogenicity-and-protective-efficacy-of-recombinant-bacille-calmette-guerin-strains-expressing-mycobacterium-antigens-ag85a-cfp10-esat-6-gm-csf-and-il-12p70
#4
Fu-Ying Dai, Jun-Fang Wang, Xue-Li Gong, Lang Bao
OBJECTIVE: This study aimed to evaluate the immunogenicity and protective efficacy of recombinant bacille calmette-guerin (rBCG) strains expressing Ag85A (A), CFP10 (C), ESAT6 (E), IL-12p70 (I), and fusion protein GM-CSF (G). METHOD: rBCGs were established by integrating of A, C, E, I, G, AE, CE, IE, GC, GE and GCE into Mycobacterium bovis BCG-1173 and BCG-SH. The macro-effects of rBCGs on mice were evaluated by phenotype and weight. The immunogenicity of rBCGs was analyzed by lgG, lgG1 and lgG2a antibody titers, and IFN-γ and IL-4 contents through Enzyme-linked immunosorbent assay (ELISA)...
February 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28274307/-esat-6-inhibits-autophagy-in-macrophages-and-promotes-the-growth-of-bcg
#5
Xuewei Xu, Runpeng Zhao, Rongbo Zhang, Jing Wu, Dong Hu, Yingru Xing, Shengfa Ni, Baoxian Tie
Objective To explore the interaction between 6 kD early secretory antigenic target (ESAT6) and autophagy in order to provide an experimental basis for the immune evasion mediated by ESAT6. Methods RAW264.7 cell lines (mouse macrophages) were treated with Earle's balanced salt solution (EBSS), and another cells were transfected by control plasmid pCMV-HA or recombinant plasmid pCMV-HA-ESAT6. Then we observed the growth of Bacillus Calmette-Guerin (BCG) in macrophages. Western blotting was used to detect the LC3 levels in RAW264...
March 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28258490/relationship-of-spontaneous-pneumothorax-cases-seen-in-eastern-black-sea-region-with-meteorological-changes
#6
Mustafa Esat Yamac, Sami Karapolat, Atila Turkyilmaz, Kubra Nur Seyis, Celal Tekinbas
The relationship of climate changes or weather conditions with the incidence of pneumothorax has been explored for many years. We aimed at revealing the effects of meteorological changes on the incidence of pneumothorax in the Eastern Black Sea region where spontaneous pneumothorax cases are seen relatively more frequently. The records of 195 subjects (179 males and 16 females) who had been monitored and treated due to spontaneous pneumothorax between January 2006 and December 2012 at our clinic were reviewed retrospectively, and their relationship was investigated with the meteorological data obtained by going through the database archive records of the 11th Regional Meteorology Directorate for the years between 2006 and 2012...
March 3, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28254318/detection-of-esat-6-by-a-label-free-miniature-immuno-electrochemical-biosensor-as-a-diagnostic-tool-for-tuberculosis
#7
Mohamed Fethi Diouani, Oussama Ouerghi, Amira Refai, Kamel Belgacem, Chaker Tlili, Dhafer Laouini, Makram Essafi
Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28254165/content-validity-testing-of-the-esat-%C3%A2-a-decision-aid-tool-for-performing-endotracheal-suction-in-children
#8
K Davies, M Bulsara, A S Ramelet, L Monterosso
BACKGROUND AND PURPOSE: Endotracheal tube suction performed in children can affect clinical stability. Previous research has identified clinical indicators used to perform endotracheal suction. These were used to develop the Endotracheal Suction Assessment Tool(©) (ESAT(©)). This study sought to evaluate the degree to which the Endotracheal Suction Assessment Tool(©) items as a whole constitute an operational definition of the construct used to determine whether a paediatric intensive care nurse should perform the endotracheal tube suction procedure...
February 18, 2017: Australian Critical Care: Official Journal of the Confederation of Australian Critical Care Nurses
https://www.readbyqxmd.com/read/28221257/intravitreal-dexamethasone-implant-in-retinitis-pigmentosa-related-cystoid-macular-edema
#9
Ahmad M Mansour, Huda Sheheitli, Cem Kucukerdonmez, Robert A Sisk, Raphael Moura, Marilita M Moschos, Luiz H Lima, Laila Al-Shaar, J Fernando Arevalo, Mauricio Maia, Robert E Foster, Ozcan Kayikcioglu, Igor Kozak, Shree Kurup, Hernando Zegarra, Roberto Gallego-Pinazo, Rola N Hamam, Riad A Bejjani, Esat Cinar, Ethem T Erakgün, Alan Kimura, Anderson Teixeira
PURPOSE: To report the clinical outcome after intravitreal dexamethasone implant in patients with retinitis pigmentosa and cystoid macular edema. METHODS: Multicenter retrospective case series of eyes with retinitis pigmentosa and cystoid macular edema that underwent intravitreal dexamethasone implant. Primary outcome measures were best-corrected visual acuity in LogMAR and central macular thickness. Statistical analyses used two-tailed comparison with Wilcoxon signed-rank test...
February 17, 2017: Retina
https://www.readbyqxmd.com/read/28216751/femoral-bowing-plane-adaptation-to-femoral-anteversion
#10
Alp Akman, Fahir Demirkan, Nuran Sabir, Murat Oto, Cagdas Yorukoglu, Esat Kiter
BACKGROUND: Femoral bowing plane (FBP) is the unattended subject in the literature. More over the femoral shaft with its bowing is neglected in established anteversion determination methods. There is limited information about the relationship between FBP and anteversion. Thus we focused on this subject and hypothesized that there could be an adaptation of FBP to anteversion. MATERIALS AND METHODS: FBP is determined on three-dimensional solid models derived from the left femoral computerized tomography data of 47 patients which were taken before for another reason and comparatively evaluated with anteversion...
January 2017: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/28209712/mycobacterium-tuberculosis-esxl-inhibit-mhc-ii-expression-by-promoting-hypermethylation-in-class-ii-transactivator-loci-in-macrophages
#11
Srabasti Sengupta, Saba Naz, Ishani Das, Abdul Ahad, Avinash Padhi, Sumanta Naik, Geetanjali Ganguli, Kaliprasad Patnaik, Sunil Kumar Raghav, Vinay Nandicoori, Avinash Sonawane
Mycobacterium tuberculosis (Mtb) is known to modulate the host immune responses to facilitate its persistence inside the host cells. One of the key mechanisms includes repression of class-II transactivator (CIITA) and MHC-II expression in infected macrophages. However, the precise mechanism of CIITA and MHC-II down-regulation is not well studied. Mtb 6-kDa early secretory antigenic target (ESAT-6) is a known potent virulence and antigenic determinant. Mtb genome encodes 23 such ESAT-6 family proteins. We herein report that Mtb and M...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28193909/rv2299c-a-novel-dendritic-cell-activating-antigen-of-mycobacterium-tuberculosis-fused-esat-6-subunit-vaccine-confers-improved-and-durable-protection-against-the-hypervirulent-strain-hn878-in-mice
#12
Han-Gyu Choi, Seunga Choi, Yong Woo Back, Seungwha Paik, Hye-Soo Park, Woo Sik Kim, Hongmin Kim, Seung Bin Cha, Chul Hee Choi, Sung Jae Shin, Hwa-Jung Kim
Understanding functional interactions between DCs and antigens is necessary for achieving an optimal and desired immune response during vaccine development. Here, we identified and characterized protein Rv2299c (heat-shock protein 90 family), which effectively induced DC maturation. The Rv2299c-maturated DCs showed increased expression of surface molecules and production of proinflammatory cytokines. Rv2299c induced these effects by binding to TLR4 and stimulating the downstream MyD88-, MAPK- and NF-κB-dependent signaling pathways...
February 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28193504/strong-anti-epstein-barr-virus-ebv-or-cytomegalovirus-cmv-cellular-immune-responses-predict-survival-and-a-favourable-response-to-anti-tuberculosis-therapy
#13
REVIEW
Tumaini Nagu, Said Aboud, Martin Rao, Mecky Matee, Rebecca Axelsson, Davide Valentini, Ferdinand Mugusi, Alimuddin Zumla, Markus Maeurer
BACKGROUND: Intact immune responses to cytomegalovirus (CMV) and Epstein-Barr virus (EBV) represent a biologically and clinically relevant correlate of 'immunological fitness' in humans. However, there is a lack of knowledge concerning anti-EBV or anti-CMV responses in patients with pulmonary tuberculosis (TB), in whom aberrant immune responses may promote progression of clinical disease. METHODS: Venous blood samples were obtained at the time of (sputum smear positive) pulmonary TB diagnosis...
February 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28185617/mycobacterium-kansasii
#14
James C Johnston, Leslie Chiang, Kevin Elwood
The incidence of Mycobacterium kansasii varies widely over time and by region, but this organism remains one of the most clinically relevant isolated species of nontuberculous mycobacteria. In contrast to other common nontuberculous mycobacteria, M. kansasii is infrequently isolated from natural water sources or soil. The major reservoir appears to be tap water. Infection is likely acquired through the aerosol route, with low infectivity in regions of endemicity. Human-to-human transmission is thought not to occur...
January 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28159608/safety-and-efficacy-of-the-c-tb-skin-test-to-diagnose-mycobacterium-tuberculosis-infection-compared-with-an-interferon-%C3%AE-release-assay-and-the-tuberculin-skin-test-a-phase-3-double-blind-randomised-controlled-trial
#15
Morten Ruhwald, Henrik Aggerbeck, Rafael Vázquez Gallardo, Søren T Hoff, José I Villate, Bettine Borregaard, José A Martinez, Ingrid Kromann, Antón Penas, Luis L Anibarro, Maria Luiza de Souza-Galvão, Francisca Sánchez, Jose Ángel Rodrigo-Pendás, Antoni Noguera-Julian, Xavier Martínez-Lacasa, Maria Victoria Tuñez, Virginia Leiro Fernández, Joan P Millet, Antonio Moreno, Nazaret Cobos, José M Miró, Llanos Roldan, Angels Orcau, Peter Andersen, Joan A Caylá
BACKGROUND: Targeted screening and treatment of Mycobacterium tuberculosis infection substantially reduces the risk of developing active tuberculosis. C-Tb (Statens Serum Institute, Copenhagen, Denmark) is a novel specific skin test based on ESAT-6 and CFP10 antigens. We investigated the safety and diagnostic potential of C-Tb compared with established tests in the contact-tracing setting. METHODS: Negative controls, close contacts, occasional contacts, and patients with active pulmonary tuberculosis were enrolled at 13 centres in Spain...
January 31, 2017: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/28135876/analysis-of-the-association-between-mycobacterium-tuberculosis-infection-and-immunoglobulin-a-nephropathy-by-early-secreted-antigenic-target-6-detection-in-renal-biopsies-a-prospective-study
#16
Sun Li, Teng Siyuan, Feng Jiangmin, Wang Hailong, Yao Li, Ma Jianfei, Wang Lining
OBJECTIVES: Immunoglobulin A nephropathy (IgAN) is the most frequent cause of primary renal disease, and clarifying the pathogenesis of IgAN is of great importance for its diagnosis and treatment. It is well known that Mycobacterium tuberculosis (MTB) can infect the urinary tract and result in the typical symptoms of cystitis. However, MTB can also affect the kidney more insidiously. Patients may present with glomerular disease, and sometimes with advanced renal failure. This study was to investigate the association between MTB infection and IgA nephropathy (IgAN), and the early diagnosis of MTB-mediated IgAN by means of early secreted antigenic target 6 (ESAT-6) detection in renal biopsies...
January 30, 2017: Postgraduate Medicine
https://www.readbyqxmd.com/read/28123491/ag85b-synergizes-with-esat-6-to-induce-efficient-and-long-term-immunity-of-c57bl-6-mice-primed-with-recombinant-bacille-calmette-guerin
#17
Wei Liu, Ying Xu, Jingran Yan, Hongbo Shen, Enzhuo Yang, Honghai Wang
The latest probable scenario in vaccination strategies is to prime one live attenuated vaccine candidate followed by boost dose of second vaccine candidate. In the present study, we primed the mice with a recombinant Bacille Calmette-Guerin (BCG) comprising Ag85B and ESAT-6 followed by boost doses of Ag85B, ESAT-6 and Ag85B-ESAT-6 fusion protein in the DDA adjuvant, separately. After boost doses of 8 and 12 weeks, the levels of antigen-stimulated T cells secreting interferon (IFN)-γ, the content of the IFN-γ, tumor necrosis factor-α and interleukin-4 in the splenocytes in vitro culture supernatant, the antigen-specific immunoglobulin (Ig)G titer from mouse serum, IgG subclass and the population of antigen-specific CD4(+) and CD8(+) T cells were detected...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28122644/mtb-specific-lymphocyte-responses-are-impaired-in-tuberculosis-patients-with-pulmonary-cavities
#18
Jun Wang, Yaping Dai, Jun Liu, Yongmei Yin, Hao Pei
OBJECTIVE: Tuberculosis (TB), an infectious disease caused by the bacillus Mycobacterium tuberculosis (MTB), is a global health problem. Because the failing immune response in the lung can lead to formation of a pulmonary cavity, this study was designed to clarify MTB-specific lymphocyte responses in TB patients with pulmonary cavities. METHODS: We utilized culture filtrate protein 10 (CFP-10) and early secretory antigenic target 6 (ESAT-6) as immunogenic MTB antigens following overnight stimulation of peripheral blood mononuclear cells (PBMCs)...
January 26, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28119503/mycobacterial-esx-1-secretion-system-mediates-host-cell-lysis-through-bacterium-contact-dependent-gross-membrane-disruptions
#19
William H Conrad, Morwan M Osman, Jonathan K Shanahan, Frances Chu, Kevin K Takaki, James Cameron, Digby Hopkinson-Woolley, Roland Brosch, Lalita Ramakrishnan
Mycobacterium tuberculosis and Mycobacterium marinum are thought to exert virulence, in part, through their ability to lyse host cell membranes. The type VII secretion system ESX-1 [6-kDa early secretory antigenic target (ESAT-6) secretion system 1] is required for both virulence and host cell membrane lysis. Both activities are attributed to the pore-forming activity of the ESX-1-secreted substrate ESAT-6 because multiple studies have reported that recombinant ESAT-6 lyses eukaryotic membranes. We too find ESX-1 of M...
February 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28115506/characterization-of-t-cells-specific-for-cfp-10-and-esat-6-in-m-tuberculosis-infected-mauritian-cynomolgus-macaques
#20
Amy Ellis, Alexis Balgeman, Mark Rodgers, Cassaundra Updike, Jaime Tomko, Pauline Maiello, Charles A Scanga, Shelby L O'Connor
Nonhuman primates can be used to study host immune responses to Mycobacterium tuberculosis (Mtb). Mauritian cynomolgus macaques (MCMs) are a unique group of animals that have limited MHC genetic diversity, such that MHC-identical animals can be infected with Mtb. Two MCMs homozygous for the relatively common M1 MHC haplotype were bronchoscopically infected with 41 colony-forming units (CFU) Mtb, Erdman strain. Four other MCMs, which had at least one copy of the M1 MHC haplotype, were infected with a lower dose of 3 CFU Mtb...
January 23, 2017: Infection and Immunity
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