keyword
https://read.qxmd.com/read/33554959/a-simple-dry-sectioning-method-for-obtaining-whole-seed-sized-resin-section-and-its-applications
#21
JOURNAL ARTICLE
Jiajing Qiu, Yinhui Ren, Lingxiao Zhao, Biao Zhang, Cunxu Wei
The morphology, size and quantity of cells, starch granules and protein bodies in seed determine the weight and quality of seed. They are significantly different among different regions of seed. In order to view the morphologies of cells, starch granules and protein bodies clearly, and quantitatively analyze their morphology parameters accurately, the whole-seed-sized section is needed. Though the whole-seed-sized paraffin section can investigate the accumulation of storage materials in seeds, it is very difficult to quantitatively analyze the morphology parameters of cells and storage materials due to the low resolution of the thick section...
January 23, 2021: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/33382971/perspectives-on-geoff-burnstock-as-researcher-teacher-and-friend
#22
REVIEW
Maria P Abbracchio
Geoffrey Burnstock, one of the most talented and brilliant scientists of his generation, was born on the 10th of May 1929 in London and died on the 2nd of June 2020, aged 91, in Melbourne (Australia). Geoffrey Burnstock started his research studies with an interest in the nerves controlling the guts of guinea pigs, and discovered a completely unexpected and ubiquitous signalling system mediated via extracellular nucleotides (the "purinergic theory"), which revolutionized our understanding of how cells communicate between each other...
December 28, 2020: Biochemical Pharmacology
https://read.qxmd.com/read/33316468/efficient-self-photo-degradation-of-cationic-textile-dyes-involved-triethylamine-and-degradation-pathway
#23
JOURNAL ARTICLE
Cheng-Bo Li, Fenghua Xiao, Wenhua Xu, Yilong Chu, Qian Wang, Haiying Jiang, Kebin Li, Xue-Wang Gao
Cationic textile dyes such as astrazon brilliant red (ABR), are frequently used in the textile industry and contaminait the water ecology. Photodegradation of such dyes in wastewater is considered as a promising method, while the existing approaches are usually involved complicated and costly materials as photocatalysts. Facial, effective and low-cost approaches for their decontamination are needed. What's more, the detailed decomposition path of ABR is not revealed. The present study shows that ABR could suffer effective self-photo-degradation under triethylamine treatment without a photocatalyst...
December 5, 2020: Chemosphere
https://read.qxmd.com/read/33228232/ligninolytic-enzyme-production-and-decolorization-capacity-of-synthetic-dyes-by-saprotrophic-white-rot-brown-rot-and-litter-decomposing-basidiomycetes
#24
JOURNAL ARTICLE
Ivana Eichlerová, Petr Baldrian
An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi...
November 19, 2020: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/33165707/the-history-of-the-purine-club-a-tribute-to-prof-geoffrey-burnstock
#25
REVIEW
Maria P Abbracchio
The international purinergic scientific community has lost its pioneer. Geoffrey Burnstock, born on the 10th of May 1929 in London, died on the 2nd of June 2020, aged 91, in Melbourne (Australia). Geoff was one of the most highly regarded scientists of his generation. In the 1960s and 1970s, he developed a radical and somehow heretical new theory and opened an entire new field of science, signalling via extracellular nucleotides (the "purinergic theory"), which revolutionized our understanding of how cells communicate between each other...
November 9, 2020: Purinergic Signalling
https://read.qxmd.com/read/33131941/biodegradation-and-metabolic-pathway-of-anthraquinone-dyes-by-trametes-hirsuta-d7-immobilized-in-light-expanded-clay-aggregate-and-cytotoxicity-assessment
#26
JOURNAL ARTICLE
Rafiqul Alam, Fenny Clara Ardiati, Nissa Nurfajrin Solihat, Md Badrul Alam, Sang Han Lee, Dede Heri Yuli Yanto, Takashi Watanabe, Sunghwan Kim
Biodegradation and metabolic pathways of three anthraquinone dyes, Reactive Blue 4 (RB4), Remazol Brilliant Blue - R (RBBR), and Acid Blue 129 (AB129) by Trametes hirsuta D7 fungus immobilized in light expanded clay aggregate (LECA) were investigated. Morphological characteristics observed with scanning electron microscope (SEM) showed successful immobilization of the fungus in LECA. Based on UV absorbance measurement, immobilized T. hirsuta D7 effectively degraded 90%, 95%, and 96% of RB4, RBBR and AB129, respectively...
October 15, 2020: Journal of Hazardous Materials
https://read.qxmd.com/read/32589252/-step-by-step-principles-of-safe-laparoscopic-approach-with-technical-details-in-median-arcuate-ligament-syndrome
#27
JOURNAL ARTICLE
Selçuk Gülmez
The median arcuate ligament syndrome (MALS) is a rare cause of postprandial pain and weight loss. The median arcuate ligament (MAL) is a fibrous band of the diaphragmatic crura. Abnormally downward located MAL or high take off of the celiac artery result in external compression the celiac trunk. MAL narrows the truncus coeliacus even more clearly during the expiration. The chronic compression of the celiac artery reduces blood flow and causes symptoms. Symptomatic patients receive surgical treatment, in recent years, an increasing rate, especially laparoscopic...
July 2020: Turkish Journal of Trauma & Emergency Surgery: TJTES
https://read.qxmd.com/read/32341936/k-edge-subtraction-imaging-for-iodine-and-calcium-separation-at-a-compact-synchrotron-x-ray-source
#28
JOURNAL ARTICLE
Stephanie Kulpe, Martin Dierolf, Eva-Maria Braig, Benedikt Günther, Klaus Achterhold, Bernhard Gleich, Julia Herzen, Ernst Rummeny, Franz Pfeiffer, Daniela Pfeiffer
Purpose: About one third of all deaths worldwide can be traced to some form of cardiovascular disease. The gold standard for the diagnosis and interventional treatment of blood vessels is digital subtraction angiography (DSA). An alternative to DSA is K-edge subtraction (KES) imaging, which has been shown to be advantageous for moving organs and for eliminating image artifacts caused by patient movement. As highly brilliant, monochromatic x-rays are required for this method, it has been limited to synchrotron facilities so far, restraining the applicability in the clinical routine...
March 2020: Journal of Medical Imaging
https://read.qxmd.com/read/32152254/-my-story-of-recovery-from-brain-damage
#29
JOURNAL ARTICLE
Keiko Seki
The author (hereafter referred to as I) is a speech-language-hearing pathologist and former professor of neuropsychology at Kobe University, who had been engaged in neuropsychological rehabilitation and research of conditions, such as aphasia and unilateral spatial neglect, for 37 years. In July 2009, I experienced right hemispheric infarction in Kobe City, and developed left-sided hemiparesis and various symptoms of cognitive dysfunctions, which was previously my field of expertise. As a neuropsychological rehabilitation expert, I focused my efforts on recovery and I have recovered the majority of my cognitive and neurologic functions...
March 2020: Brain and Nerve, Shinkei Kenkyū No Shinpo
https://read.qxmd.com/read/32093950/doctors-in-star-trek-dr-julian-bashir-in-star-trek-deep-space-9
#30
JOURNAL ARTICLE
Victor Grech
Doctors are pivotal characters in Star Trek. This paper will review Dr. Julian Bashir, a unique medic who is genetically engineered to superhuman capacities while deliberately designed to have human failings, which he successfully overcomes. His genetic enhancements allow him to excel in whatever he does and although initially portrayed as callow, arrogant, brash and naïve, his open mind permits him to eventually discard these negative traits to become a more rounded, if superhuman, doctor, who forms staunch friendships among the crew of the space station...
May 2020: Early Human Development
https://read.qxmd.com/read/31811873/lsp5-2157-a-new-inhibitor-of-vesicular-glutamate-transporters
#31
JOURNAL ARTICLE
Odile Poirel, Lauren E Mamer, Melissa A Herman, Marie Arnulf-Kempcke, Myriam Kervern, Brigitte Potier, Stephanie Miot, Jing Wang, Franck-Cyril Favre-Besse, Isabelle Brabet, Younès Laras, Hugues-Olivier Bertrand, Francine Acher, Jean-Philippe Pin, Jean-Luc Puel, Bruno Giros, Jacques Epelbaum, Christian Rosenmund, Patrick Dutar, Stephanie Daumas, Salah El Mestikawy, Nicolas Pietrancosta
Vesicular glutamate transporters (VGLUT1-3) mediate the uptake of glutamate into synaptic vesicles. VGLUTs are pivotal actors of excitatory transmission and of almost all brain functions. Their implication in various pathologies has been clearly documented. Despite their functional importance, the pharmacology of VGLUTs is limited to a few dyes such as Trypan Blue, Rose Bengal or Brilliant Yellow type. Here, we report the design and evaluation of new potent analogs based on Trypan Blue scaffold. Our best compound, named LSP5-2157, has an EC50 of 50 nM on glutamate vesicular uptake...
December 4, 2019: Neuropharmacology
https://read.qxmd.com/read/31763771/simultaneous-polychromatic-immunofluorescent-staining-of-tissue-sections-and-consecutive-imaging-of-up-to-seven-parameters-by-standard-confocal-microscopy
#32
JOURNAL ARTICLE
Alfonso J Schmidt, Johannes U Mayer, Paul K Wallace, Franca Ronchese, Kylie M Price
Confocal microscopy has been an important imaging tool for life scientists for over 20 years. Early techniques focused on indirect staining processes that involved staining with an unconjugated primary antibody, followed by incubation with a secondary fluorescent antibody that would reveal and amplify the signal of the primary antibody. With more and more directly conjugated fluorescent primary antibodies becoming commercially available, staining with multiple fluorescent primary antibodies is now more frequent...
December 2019: Current Protocols in Cytometry
https://read.qxmd.com/read/31648063/active-transport-of-brilliant-blue-fcf-across-the-drosophila-midgut-and-malpighian-tubule-epithelia
#33
JOURNAL ARTICLE
Dawson B H Livingston, Hirva Patel, Andrew Donini, Heath A MacMillan
Under conditions of stress, many animals suffer from epithelial barrier disruption that can cause molecules to leak down their concentration gradients, potentially causing a loss of organismal homeostasis, further injury or death. Drosophila is a common insect model, used to study barrier disruption related to aging, traumatic injury, or environmental stress. Net leak of a non-toxic dye (Brilliant blue FCF) from the gut lumen to the hemolymph is often used to identify barrier failure under these conditions, but Drosophila are capable of actively transporting structurally-similar compounds...
October 21, 2019: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://read.qxmd.com/read/30995753/laccase-immobilized-onto-zirconia%C3%A2-silica-hybrid-doped-with-cu-2-as-an-effective-biocatalytic-system-for-decolorization-of-dyes
#34
JOURNAL ARTICLE
Katarzyna Jankowska, Filip Ciesielczyk, Karolina Bachosz, Jakub Zdarta, Ewa Kaczorek, Teofil Jesionowski
Nowadays, novel and advanced methods are being sought to efficiently remove dyes from wastewaters. These compounds, which mainly originate from the textile industry, may adversely affect the aquatic environment as well as living organisms. Thus, in presented study, the synthesized ZrO2 -SiO2 and Cu2+ -doped ZrO2 -SiO2 oxide materials were used for the first time as supports for laccase immobilization, which was carried out for 1 h, at pH 5 and 25 °C. The materials were thoroughly characterized before and after laccase immobilization with respect to electrokinetic stability, parameters of the porous structure, morphology and type of surface functional groups...
April 16, 2019: Materials
https://read.qxmd.com/read/30624815/a-diketopyrrolopyrrole-based-dimer-as-blue-pigment
#35
JOURNAL ARTICLE
Manuela Casutt, Benedikt Dittmar, Hanna Makowska, Tomasz Marszalek, Soh Kushida, Uwe H F Bunz, Jan Freudenberg, Daniel Jaensch, Klaus Müllen
1,4-Diketopyrrolo[3,4-c]pyrroles (DPPs) constitute a class of hydrogen-bonded pigments covering a color range from yellow-orange to dark violet. We report facile dimerization of a Boc-substituted 3,6-dithienyl-DPP. The formed dimer shows brilliantly blue color as a result of the expansion of the chromophore. The soluble dimer acts as latent pigment and is converted to the corresponding pigment either via thermocleavage or acidic deprotection of the Boc groups. The synthesized pigment is characterized by high migrational stability in a polymer matrix and a clear blue color...
January 9, 2019: Chemistry: a European Journal
https://read.qxmd.com/read/30547459/application-of-gelatin-zymography-in-nanotoxicity-research
#36
JOURNAL ARTICLE
Yue Zhang, Rong Wan, Qunwei Zhang, Yiqun Mo
Gelatin zymography is a relatively simple, inexpensive, and powerful technique to detect proteolytic enzymes capable of degrading gelatin from various biological sources. It has been used particularly to detect the two members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. MMP-2 and MMP-9 are also able to degrade a number of extracellular matrix molecules including type IV, V, and XI collagens, laminin, and aggrecan core protein, thus making them important in the nanotoxicity research...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30097950/fungal-laccase-efficiently-destains-coomassie-brilliant-blue-r-250-stained-polyacrylamide-gels
#37
JOURNAL ARTICLE
Gaurav Kumar
Fungal laccase-mediated destaining of Coomassie Brilliant Blue-R-250 stained polyacrylamide electrophoretic gel is very sensitive and environment friendly. Fermenting of Cerrena sp. HYB07 yields a broth with laccase activity which when supplemented with distilled water and used as a destaining solution results in a clear gel background in 2 h at 37 °C. Also, separation of the laccase enzyme for efficient functioning is not required. The method utilizes gel destaining and process of decolorizing dye simultaneously with a detection sensitivity of up to 10 ng...
2018: Methods in Molecular Biology
https://read.qxmd.com/read/30042988/structurally-colored-polymer-films-with-narrow-stop-band-high-angle-dependence-and-good-mechanical-robustness-for-trademark-anti-counterfeiting
#38
JOURNAL ARTICLE
Zhipeng Meng, Suli Wu, Bingtao Tang, Wei Ma, Shufen Zhang
The photonic stop bands of colloidal crystals appear as structural colors, which are potentially useful for display devices, colorimetric sensors, optical filters, paints, and photonic papers. However, low durability and pale colors caused by the undesired scattering of light seriously limit their practical applications. In this article, a polydimethylsiloxane (PDMS)/photonic crystal (PC)/PDMS sandwich structure was designed as a free standing structural colored film with good durability and brilliant color...
August 2, 2018: Nanoscale
https://read.qxmd.com/read/29656164/lignolytic-mushroom-lenzites-elegans-wdp2-laccase-production-characterization-and-bioremediation-of-synthetic-dyes
#39
JOURNAL ARTICLE
Raj Kumar Pandey, Salil Tewari, Lakshmi Tewari
A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1 ) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189...
August 30, 2018: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/29489698/never-too-old-to-fight-breast-cancer-a-case-report
#40
JOURNAL ARTICLE
Monica Zuradelli, Giovanna Masci, Emanuela Ferraro, Agnese Losurdo, Rita De Sanctis, Rosalba Torrisi, Armando Santoro
RATIONALE: Breast cancer is the most common cancer affecting females worldwide and its lifetime risk increases with age. Human epidermal growth factor receptor gene-2 (HER-2) positive breast cancer represents about 20% of all breast cancers, 1 out of 10 is diagnosed in women over 70 years of age. It tends to be more aggressive and to spread more quickly than other subtypes, but the introduction in clinical practice of new anti-HER-2 agents combined with chemotherapy has significantly improved progression free and overall survival...
March 2018: Medicine (Baltimore)
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