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https://www.readbyqxmd.com/read/29656164/lignolytic-mushroom-lenzites-elegans-wdp2-laccase-production-characterization-and-bioremediation-of-synthetic-dyes
#1
Raj Kumar Pandey, Salil Tewari, Lakshmi Tewari
A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1 ) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189...
April 12, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29489698/never-too-old-to-fight-breast-cancer-a-case-report
#2
Monica Zuradelli, Giovanna Masci, Emanuela Ferraro, Agnese Losurdo, Rita De Sanctis, Rosalba Torrisi, Armando Santoro
RATIONALE: Breast cancer is the most common cancer affecting females worldwide and its lifetime risk increases with age. Human epidermal growth factor receptor gene-2 (HER-2) positive breast cancer represents about 20% of all breast cancers, 1 out of 10 is diagnosed in women over 70 years of age. It tends to be more aggressive and to spread more quickly than other subtypes, but the introduction in clinical practice of new anti-HER-2 agents combined with chemotherapy has significantly improved progression free and overall survival...
March 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29432751/an-efficient-screening-method-for-purifying-and-crystallizing-membrane-proteins-using-modified-clear-native-page
#3
Nanao Suzuki, Yuuki Takamuku, Tomohiro Asakawa, Makoto Inai, Tomoya Hino, So Iwata, Toshiyuki Kan, Takeshi Murata
Membrane proteins, such as G-protein coupled receptors, control communication between cells and their environments and are indispensable for many cellular functions. Nevertheless, structural studies on membrane proteins lag behind those on water-soluble proteins, due to their low structural stability, making it difficult to obtain crystals for X-ray crystallography. Optimizing conditions to improve the stability of membrane proteins is essential for successful crystallization. However, the optimization usually requires large amounts of purified samples, and it is a time-consuming and trial-and-error process...
February 9, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29072221/comparative-evaluation-of-inhibitory-effect-of-curcumin-and-doxycycline-on-matrix-metalloproteinase-9-activity-in-chronic-periodontitis
#4
Sanjeela Rakshith Guru, Shaila V Kothiwale, Nitin Saroch, Rakshith Chander Guru
BACKGROUND AND OBJECTIVES: The pathogenesis of inflammatory periodontal diseases essentially involves degradation of extracellular matrix molecules, and collagen breakdown and matrix metalloproteinases (MMPs) are proteinases primarily involved in this process. It is known that doxycycline downregulates MMP activity. Curcumin has anti-inflammatory effect and also downregulates MMP activity. Thus, a study was conducted to evaluate the anti-inflammatory effect of curcumin by its inhibition of MMP-9 activity and compare the same with doxcycline, which has known anticollagenase activity...
September 2017: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/29031258/ultrafast-and-nonlinear-spectroscopy-of-brilliant-green-based-nanogumbos-with-enhanced-near-infrared-emission
#5
Tony E Karam, Noureen Siraj, Zhenyu Zhang, Abdulrahman F Ezzir, Isiah M Warner, Louis H Haber
The synthesis, characterization, ultrafast dynamics, and nonlinear spectroscopy of 30 nm nanospheres of brilliant green-bis(pentafluoroethylsulfonyl)imide ([BG][BETI]) in water are reported. These thermally stable nanoparticles are derived from a group of uniform materials based on organic salts (nanoGUMBOS) that exhibit enhanced near-infrared emission compared with the molecular dye in water. The examination of ultrafast transient absorption spectroscopy results reveals that the overall excited-state relaxation lifetimes of [BG][BETI] nanoGUMBOS are longer than the brilliant green molecular dye in water due to steric hindrance of the torsional degrees of freedom of the phenyl rings around the central carbon...
October 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28690673/professor-umberto-veronesi-a-physician-a-researcher-a-brilliant-man
#6
Ketti Mazzocco, Chiara Marzorati, Gabriella Pravettoni
Many of those who work in oncology or deal with cancer patients know of Prof. Umberto Veronesi and none of them could deny the importance of his battle against cancer. He devoted his life to improving cancer treatment and quality of life for patients. He was a physician, and a politician, but above all he was a researcher. He embodied the true spirit of research, i.e., to believe in something and investigate every aspect of it until all the questions about it have been satisfactorily answered. He never gave up when faced with challenges, and he never stopped being curious...
2017: Ecancermedicalscience
https://www.readbyqxmd.com/read/28483170/-did-mozart-suffer-from-gilles-de-la-tourette-syndrome
#7
Leonardo Palacios-Sánchez, Juan Sebastián Botero-Meneses, Laura Daniela Vergara-Méndez, Natalia Pachón, Arianna Martínez, Santiago Ramírez
The personal and private lives of great men and women in history, like writers, painters and musicians, have been the subject of great interest for many years. A clear example of this is the vast scrutiny is cast over the famous composer, Wolfgang Amadeus Mozart. What may have started as curiosity, rapidly evolved into extensive research, as the answers about the musician's legendary talent may lie in the details of his life (his childhood, his relationships, his quirks and his mannerisms). It is usually up to historians, anthropologists or philosophers to delve into the pages of old books, trying to grasp answers and clues...
April 2017: Revista Colombiana de Psiquiatría
https://www.readbyqxmd.com/read/28465179/phenoloxidase-activity-in-humoral-plasma-hemocyanin-and-hemocyanin-separated-proteins-of-the-giant-freshwater-prawn-macrobrachium-rosenbergii
#8
Sivakumar Mullaivanam Ramasamy, Maghil Denis, Shanthi Sivakumar, Arumugam Munusamy
Hemocyanin is a copper containing protein and its role in the immune function of phenoloxidase (PO) activity was investigated in the giant freshwater prawn Macrobrachium rosenbergii. Hemocyanin, sedimented by ultracentrifugation from the plasma appeared on polyacrylamide gel electrophoresis (PAGE 7%) on Coomassie Brilliant Blue and bathocuproine sulfonic acid stain as four copper containing proteins of molecular masses 50, 60, 114 and 325kDa. Accordingly, on diethylaminoethyl-cellulose anion exchange column hemocyanin separated into four proteins designated as MrHc1, MrHc2, MrHc3 and MrHc4 with electrophoretically (PAGE) determined molecular masses of 60, 114, 50 and 325kDa respectively...
September 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28344198/radiosensitizing-effect-of-p2x7-receptor-antagonist-on-melanoma-in-vitro-and-in-vivo
#9
Keisuke Tanamachi, Keisuke Nishino, Natsuki Mori, Toshihiro Suzuki, Sei-Ichi Tanuma, Ryo Abe, Mitsutoshi Tsukimoto
Melanoma is highly malignant, and generally exhibits radioresistance, responding poorly to radiation therapy. We previously reported that activation of P2X7, P2Y6, and P2Y12 receptors is involved in the DNA damage response after γ-irradiation of human lung adenocarcinoma A549 cells. However, it is not clear whether these receptors are also involved in the case of melanoma cells, although P2X7 receptor is highly expressed in various cancers, including melanoma. Here, we show that P2X7 receptor antagonist enhances radiation-induced cytotoxicity in B16 melanoma cells in vitro and in vivo...
March 24, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28134994/retrospective-analysis-of-the-treatment-of-melasma-lesions-exhibiting-increased-vascularity-with-the-595-nm-pulsed-dye-laser-combined-with-the-1927-nm-fractional-low-powered-diode-laser
#10
REVIEW
Elizabeth R C Geddes, Ashlyn B Stout, Paul M Friedman
BACKGROUND: Melasma presents a significant challenge to laser surgeons. Aggressive treatments often result in rebound melasma or post-inflammatory pigmentary alteration. Recent reports suggest melasma pathogenesis may have a vascular component. Spectrocolorimetry can detect subtle or sub-clinical telangiectatic erythema within melasma lesions. For certain patients identified by spectrocolorimetry, effective melasma treatment may include vascular-targeted therapy together with pigment-specific treatment modalities...
January 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28031697/clinical-and-histological-evaluation-of-large-macular-hole-surgery-using-the-inverted-internal-limiting-membrane-flap-technique
#11
Satoru Kase, Wataru Saito, Shohei Mori, Michiyuki Saito, Ryo Ando, Zhenyu Dong, Tomohiro Suzuki, Kousuke Noda, Susumu Ishida
PURPOSE: The aims of this study were to analyze optical coherence tomography (OCT) imaging of large macular holes (MHs) treated with inverted internal limiting membrane (ILM) flap technique and to perform a histological examination of an ILM-like membrane tissue obtained during vitrectomy. PATIENTS AND METHODS: This is a retrospective observational case study. Nine patients, comprising of five males and four females, showing large and myopic MHs, underwent pars plana vitrectomy (PPV) with inverted ILM flap technique assisted by brilliant blue G (BBG) staining...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27927898/the-full-breadth-of-mendel-s-genetics
#12
Peter J van Dijk, T H Noel Ellis
Gregor Mendel's "Experiments on Plant Hybrids" (1865/1866), published 150 years ago, is without doubt one of the most brilliant works in biology. Curiously, Mendel's later studies on Hieracium (hawkweed) are usually seen as a frustrating failure, because it is assumed that they were intended to confirm the segregation ratios he found in Pisum Had this been his intention, such a confirmation would have failed, since, unknown to Mendel, Hieracium species mostly reproduce by means of clonal seeds (apomixis). Here we show that this assumption arises from a misunderstanding that could be explained by a missing page in Mendel's first letter to Carl Nägeli...
December 2016: Genetics
https://www.readbyqxmd.com/read/27881091/conducting-a-large-multi-site-survey-about-patients-views-on-broad-consent-challenges-and-solutions
#13
Maureen E Smith, Saskia C Sanderson, Kyle B Brothers, Melanie F Myers, Jennifer McCormick, Sharon Aufox, Martha J Shrubsole, Nanibaá A Garrison, Nathaniel D Mercaldo, Jonathan S Schildcrout, Ellen Wright Clayton, Armand H Matheny Antommaria, Melissa Basford, Murray Brilliant, John J Connolly, Stephanie M Fullerton, Carol R Horowitz, Gail P Jarvik, Dave Kaufman, Terri Kitchner, Rongling Li, Evette J Ludman, Catherine McCarty, Valerie McManus, Sarah Stallings, Janet L Williams, Ingrid A Holm
BACKGROUND: As biobanks play an increasing role in the genomic research that will lead to precision medicine, input from diverse and large populations of patients in a variety of health care settings will be important in order to successfully carry out such studies. One important topic is participants' views towards consent and data sharing, especially since the 2011 Advanced Notice of Proposed Rulemaking (ANPRM), and subsequently the 2015 Notice of Proposed Rulemaking (NPRM) were issued by the Department of Health and Human Services (HHS) and Office of Science and Technology Policy (OSTP)...
November 24, 2016: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/27775728/photonic-multilayer-structure-of-begonia-chloroplasts-enhances-photosynthetic-efficiency
#14
Matthew Jacobs, Martin Lopez-Garcia, O-Phart Phrathep, Tracy Lawson, Ruth Oulton, Heather M Whitney
Enhanced light harvesting is an area of interest for optimizing both natural photosynthesis and artificial solar energy capture(1,2). Iridescence has been shown to exist widely and in diverse forms in plants and other photosynthetic organisms and symbioses(3,4), but there has yet to be any direct link demonstrated between iridescence and photosynthesis. Here we show that epidermal chloroplasts, also known as iridoplasts, in shade-dwelling species of Begonia(5), notable for their brilliant blue iridescence, have a photonic crystal structure formed from a periodic arrangement of the light-absorbing thylakoid tissue itself...
October 24, 2016: Nature Plants
https://www.readbyqxmd.com/read/27694333/waardenburg-shah-syndrome-a-rare-case-in-an-indian-child
#15
Rajesh Pattebahadur, Shipra Singhi, Prafulla Kumar Maharana
A 7-year-old male child presented with a history of discolouration of right eye since birth. On examination visual acuity was 6/6 on Snellen's chart in both eyes; anterior segment was within normal limits except for the brilliant blue discolouration of the inferior quadrant and superior quadrant of right iris and left eye iris, respectively. Both eyes had a clear lens and fundus findings were within normal limits. A detailed history from parents revealed that the child had difficulty in hearing and slurring of speech...
September 30, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27327644/the-effect-of-ionic-co-presence-on-the-structural-optical-and-photocatalytic-properties-of-modified-cobalt-titanate-nanotubes
#16
B Barrocas, A J Silvestre, A G Rolo, O C Monteiro
With the aim of producing materials with enhanced optical and photocatalytic properties, titanate nanotubes (TNTs) modified by cobalt doping (Co-TNT) and by Na(+)→ Co ion-exchange (TNT/Co) were successfully prepared by a hydrothermal method. The influence of the doping level and of the cobalt position in the TNT crystalline structure was studied. Although no perceptible influence of the cobalt ion position on the morphology of the prepared titanate nanotubes was observed, the optical behaviour of the cobalt modified samples is clearly dependent on the cobalt ions either substituting the Ti(4+) ions in the TiO6 octahedra building blocks of the TNT structure (doped samples) or replacing the Na(+) ions between the TiO6 interlayers (ion-exchange samples)...
July 21, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27183547/comparative-evaluation-of-retinal-nerve-fiber-layer-thickness-after-conventional-brilliant-blue-assisted-internal-limiting-membrane-peeling-versus-brilliant-blue-selective-staining-using-whole-blood-in-macular-hole-surgery
#17
RANDOMIZED CONTROLLED TRIAL
Supriya Arora, Neha Goel, Tarun Arora, Prateeksha Sharma, Usha Kaul Raina, Meenakshi Thakar, Basudeb Ghosh
BACKGROUND AND OBJECTIVE: To evaluate retinal nerve fiber layer (RNFL) thickness after conventional brilliant blue (BB) assisted macular hole (MH) surgery versus BB selective staining using whole blood (WB) in MH surgery. PATIENTS AND METHODS: Sixty eyes with stage 4 idiopathic MH with a clear media were randomly divided into two equal groups. Group A eyes underwent sequential intraoperative use of autologous heparinized WB followed by BB dye for staining internal limiting membrane, whereas eyes in group B were subjected to conventional BB staining...
May 1, 2016: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/26985877/chromatographic-and-spectroscopic-identification-and-recognition-of-ammoniacal-cochineal-dyes-and-pigments
#18
A Chieli, J Sanyova, B Doherty, B G Brunetti, C Miliani
In this work a combined chromatographic and spectroscopic approach is used to provide a diagnostic assessment of semi-synthetic ammoniacal cochineal through the syntheses of its dyes and lakes according to art historical recipes. Commercially introduced in the late XIX century as a dye and pigment, it was used to obtain a brilliant purplish/violet nuance which provided a more stable option over carminic acid although its evidenced use in manufacts and artworks of heritage importance have been scarcely documented...
June 5, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/26846422/professor-andr%C3%A3-jouve-1909-2001-a-french-cardiologist-who-was-a-pioneer-of-clinical-vector-electrocardiography-and-cardiovascular-epidemiology
#19
Paolo Emilio Puddu
André, Julien, Auguste Jouve was born in Marseilles on June 10, 1909 son of Xavier Marie Francois Louis Jouve MD and Marie Louise Charlotte Vigliengo his wife. He had a brilliant medical career in Marseilles: Resident at Marseilles Hospitals in 1931, major of his promotion, then an Assistant in 1943 and a Chief in 1951, to become Associate Professor of Medicine in 1946 and finally Full Professor of Clinical and Experimental Cardiology in 1954. Fellow of several Cardiological Societies, he became President of the French Society of Cardiology in 1968, Vice-President of the European Society of Cardiology in 1972 and finally President of the French College of Vascular Pathology in 1973...
March 2016: Journal of Electrocardiology
https://www.readbyqxmd.com/read/26566484/hirschsprung-s-disease-historical-notes-and-pathological-diagnosis-on-the-occasion-of-the-100-th-anniversary-of-dr-harald-hirschsprung-s-death
#20
REVIEW
Consolato Sergi
Hirschsprung's disease (HSCR) or congenital megacolon is one of the differential diagnoses of chronic constipation mostly in infancy and may indeed represent a challenge for pediatricians, pediatric surgeons, and pediatric pathologists. The diagnosis relies clearly on the identification of the absence of ganglion cells at the plexuses (submucosus and myentericus) of the bowel wall. HSCR is usually located at the terminal (distal) rectum with potential pre-terminal or proximal extension to the less distal large bowel (sigmoid colon)...
November 8, 2015: World Journal of Clinical Pediatrics
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