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https://www.readbyqxmd.com/read/28222174/slmapk3-enhances-tolerance-to-tomato-yellow-leaf-curl-virus-tylcv-by-regulating-salicylic-acid-and-jasmonic-acid-signaling-in-tomato-solanum-lycopersicum
#1
Yunzhou Li, Lei Qin, Jingjing Zhao, Tayeb Muhammad, Hehe Cao, Hailiang Li, Yan Zhang, Yan Liang
Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus...
2017: PloS One
https://www.readbyqxmd.com/read/28212256/hepatic-epithelioid-hemangioendothelioma-and-adult-liver-transplantation-proposal-for-a-prognostic-score-based-on-the-analysis-of-the-eltr-elita-registry
#2
Quirino Lai, Estelle Feys, Vincent Karam, Rene Adam, Jurgen Klempnauer, Martin Oliverius, Vincenzo Mazzaferro, Andreas Pascher, Piotr Remiszewski, Helena Isoniemi, Jacques Pirenne, Aksel Foss, Bo G Ericzon, Sasa Markovic, Jan P Lerut
BACKGROUND: Hepatic epithelioid hemangioendothelioma (HEHE) is a rare vascular tumor which has an intermediate aggressive behavior. Although the value of liver transplantation (LT) is well established, its place in the management of HEHE is still unclear. The aim of this study is to confirm, based on a very large patient cohort, the value of LT in the management of HEHE and to identify risk factors for post-LT recurrence. METHODS: The outcome of 149 transplant recipients with HEHE recorded in the European Liver Transplant Registry during the period November 1984 to May 2014 was analyzed...
2017: Transplantation
https://www.readbyqxmd.com/read/28059197/in-situ-measurement-of-the-topological-charge-of-a-perfect-vortex-using-the-phase-shift-method
#3
Haixiang Ma, Xinzhong Li, Yuping Tai, Hehe Li, Jingge Wang, Miaomiao Tang, Yishan Wang, Jie Tang, Zhaogang Nie
We propose a method to determine the topological charge (TC) of a perfect vortex. With the phase shift technique, the perfect vortex and its conjugate beam exactly overlap and interfere. Consequently, the TC of a perfect vortex is determined by counting the number of interference fringes. This proposed method enables in situ determination of the TC of the perfect vortex without the need for additional optical elements, and it is immune to environmental vibration and parasitic interference.
January 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28027642/direct-blow-spinning-of-nanofibers-on-a-window-screen-for-highly-efficient-pm2-5-removal
#4
Bilal Khalid, Xiaopeng Bai, Hehe Wei, Ya Huang, Hui Wu, Yi Cui
Particulate matter (PM) pollution has caused many serious public health issues. Whereas indoor air protection usually relies on expensive and energy-consuming filtering devices, direct PM filtration by window screens has attracted increasing attention. Recently, electrospun polymer nanofiber networks have been developed as transparent filters for highly efficient PM2.5 removal; however, it remains challenging to uniformly coat the nanofibers on window screens on a large scale and with low cost. Here, we report a blow-spinning technique that is fast, efficient, and free of high voltages for the large-scale direct coating of nanofibers onto window screens for indoor PM pollution protection...
January 4, 2017: Nano Letters
https://www.readbyqxmd.com/read/27926594/hepatic-epithelioid-hemangio-endothelioma-and-adult-liver-transplantation-proposal-for-a-prognostic-score-based-on-the-analysis-of-the-eltr-elita-registry
#5
Quirino Lai, Estelle Feys, Vincent Karam, Rene Adam, Jurgen Klempnauer, Martin Oliverius, Vincenzo Mazzaferro, Andreas Pascher, Piotr Remiszewski, Helena Isoniemi, Jacques Pirenne, Aksel Foss, Bo G Ericzon, Sasa Markovic, Jan Lerut
BACKGROUND: Hepatic epithelioid hemangio-endothelioma (HEHE) is a rare vascular tumor which has an intermediate aggressive behavior. Although the value of liver transplantation (LT) is well established, its place in the management of HEHE is still unclear. The aim of this study is to confirm, based on a very large patient cohort, the value of LT in the management of HEHE and to identify risk factors for post-LT recurrence. MATERIAL AND METHODS: the outcome of 149 transplant recipients with HEHE recorded in the European Liver Transplant Registry during the period November1984-May 2014 was analyzed...
December 6, 2016: Transplantation
https://www.readbyqxmd.com/read/27895413/hepatic-epithelioid-hemangioendothelioma-dilemma-and-challenges-in-the-preoperative-diagnosis
#6
Hai-Jie Hu, Yan-Wen Jin, Qiu-Yang Jing, Anuj Shrestha, Nan-Sheng Cheng, Fu-Yu Li
Hepatic epithelioid hemangioendothelioma (HEHE) is a rare category of vascular tumor with uncertain malignant potential. It commonly presents nonspecific and variable clinical manifestations, ranging from asymptomatic to hepatic failure. In addition, laboratory measurements and imaging features also lack specificity in the diagnosis of HEHE. The aim of the present study is to highlight the dilemma and challenges in the preoperative diagnosis of HEHE, and to enhance awareness of the range of hepatobiliary surgery available in patients with multiple hepatic nodular lesions on imaging...
November 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27830021/tongxinluo-modulates-cytokine-secretion-by-cardiac-microvascular-endothelial-cells-in-ischemia-reperfusion-injury
#7
Hehe Cui, Na Li, Xiangdong Li, Kang Qi, Qing Li, Chen Jin, Tianjie Wang, Lian Duan, Leipei Jiang, Guihao Chen, Zhigang Wang, Cong Wei, Yuejin Yang
Cardiac microvascular endothelial cells (CMECs) extensively secrete cytokines during myocardial ischemia/reperfusion injury (MIRI). Tongxinluo (TXL) has been demonstrated to preserve the function of the endothelium and myocardium against MIRI. This study was designed to identify alterations in the paracrine function of CMECs under hypoxia/reoxygenation (H/R) conditions and assess its modulation by TXL. CMECs were exposed to different concentrations of TXL for 30 min and then subjected to hypoxia and reoxygenation for 12 and 2 h, respectively...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27684805/reversible-splenial-lesion-syndrome-associated-with-lobar-pneumonia-case-report-and-review-of-literature
#8
Chunrong Li, Xiujuan Wu, Hehe Qi, Yanwei Cheng, Bing Zhang, Hongwei Zhou, Xiaohong Lv, Kangding Liu, Hong-Liang Zhang
BACKGROUND: Reversible splenial lesion syndrome (RESLES) is a rare clinico-radiological disorder with unclear pathophysiology. Clinically, RESLES is defined as reversible isolated splenial lesions in the corpus callosum, which can be readily identified by magnetic resonance imaging (MRI) and usually resolve completely over a period of time. RESLES could be typically triggered by infection, antiepileptic drugs (AEDs), poisoning, etc. More factors are increasingly recognized. METHODS AND RESULTS: We reported herein an 18-year-old female patient with lobar pneumonia who developed mental abnormalities during hospitalization...
September 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27660025/enrichment-and-verification-of-differentially-expressed-mirnas-in-bursa-of-fabricius-in-two-breeds-of-duck
#9
Jun Luo, Junying Liu, Hehe Liu, Tao Zhang, Jiwen Wang, Hua He, Chunchun Han
The bursa of Fabricius (BF) is a central humoral immune organ belonging to avian specifically. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Using Illumina next-generation sequencing, the data showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability...
September 16, 2016: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/27590857/akirin2-could-promote-the-proliferation-but-not-the-differentiation-of-duck-myoblasts-via-the-activation-of-the-mtor-p70s6k-signaling-pathway
#10
Wenqiang Sun, Huilan Huang, Shengchao Ma, Xiang Gan, Mou Zhu, Hehe Liu, Liang Li, Jiwen Wang
The Akirin gene family normally contains two members that are essential to myoblast differentiation. Noticeably, the avian Akirin gene family comprises only one gene (Akirin2), However, it remains unknown whether avian Akirin gene family still has the function of Akirin1; moreover, it is still unclear whether and how Akirin2 plays a role in myoblast proliferation and differentiation. Interestingly, the unexpected functions of duck Akirin2 were revealed in the present study. The Real-time PCR results showed that between 12 and 48h during the process of duck myoblasts differentiation, the overexpression of Akirin2 did not significantly increase the expression of myogenic regulatory factors...
October 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27449079/two-potential-recombinant-rabies-vaccines-expressing-canine-parvovirus-virion-protein-2-induce-immunogenicity-to-canine-parvovirus-and-rabies-virus
#11
Jun Luo, Hehe Shi, Yeping Tan, Xuefeng Niu, Teng Long, Jing Zhao, Qin Tian, Yifei Wang, Hao Chen, Xiaofeng Guo
Both rabies virus (RABV) and canine parvovirus (CPV) cause lethal diseases in dogs. In this study, both high egg passage Flury (HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein (G) gene were used to express the CPV virion protein 2 (VP2) gene, and were designated rHEP-VP2 and, rHEP-dG-VP2 respectively. The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury. Western blotting indicated that G protein and VP2 were expressed in vitro...
August 17, 2016: Vaccine
https://www.readbyqxmd.com/read/27446853/aggressive-recurrence-of-primary-hepatic-epithelioid-haemangioendothelioma-after-liver-transplantation
#12
Qusay A Abdoh, Asma M Alnajjar, Faisal A Abaalkhail, Mohammed Al Sebayel, Hussa F Al-Hussaini, Waleed K Al-Hamoudi, Hazem Helmy, Mohamad Almansour, Hussien A Elsiesy
HEHE is a rare neoplasm of vascular origin that occurs in the liver; UNOS reported a favorable outcome after liver transplantation in 110 patients with 1-year and 5-year survival of 80% and 64%. Case Report. A 40-year-old lady presented with a three-month history of right upper abdominal pain with nausea, vomiting, and significant loss of weight associated with scleral icterus and progressive abdominal distension. Examination revealed jaundice, hepatomegaly, and ascites. Serum bilirubin was 26.5 mg/dL and ALP was 552 CT...
2016: Canadian Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27376212/epithelioid-haemangioendothelioma-ehe-of-the-liver
#13
Abidullah Khan, Mohammad Humayun, Iqbal Haider, Wazir Muhammad Khan, Fahad Ajmal, Maimoona Ayub
A45-year previously known hypertensive lady presented with 2-year history of upper abdominal pain, heaviness, and weight loss. Her radiological assessment suggested the possibility of hepatoma or liver metastases. Considering her age, overall good health and absence of cirrhosis, a liver biopsy was taken which showed hepatic epithelioidhaemangioendothelioma (HEHE), a rare and unusual intermediate grade vascular tumor which can easily be confused with hepatoma or metastatic liver disease. To the best of their knowledge, the authors are most probably reporting the first ever case of HEHE from Pakistan with special emphasis on its clinical presentations, clinico-radiological diagnostic clues, and the management options in the light of the limited retrospective studies...
June 2016: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/27263543/characterization-of-the-key-odorants-in-chinese-zhima-aroma-type-baijiu-by-gas-chromatography-olfactometry-quantitative-measurements-aroma-recombination-and-omission-studies
#14
Yang Zheng, Baoguo Sun, Mouming Zhao, Fuping Zheng, Mingquan Huang, Jinyuan Sun, Xiaotao Sun, Hehe Li
Zhima aroma-type Baijiu with typical sesame aroma is particularly popular in northern China. To our knowledge, it is still uncertain which components are important to make contributions to its unique aroma, although a few pieces of research have reported many volatile compounds in this Baijiu. The aroma-active compounds from the Baijiu were researched in this paper. A total of 56 odorants were identified in Chinese Zhima aroma-type Baijiu by aroma extract dilution analysis (AEDA). Their odor activity values (OAVs) were determined by different quantitative measurements, and then 26 aroma compounds were further confirmed as important odorants due to their OAVs ≥ 1, and these had higher values, such as ethyl hexanoate (OAV 2691), 3-methylbutanal (2403), ethyl pentanoate (1019), and so on...
July 6, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27217705/contrast-enhanced-ultrasound-of-histologically-proven-hepatic-epithelioid-hemangioendothelioma
#15
Yi Dong, Wen-Ping Wang, Vito Cantisani, Mirko D'Onofrio, Andre Ignee, Lorenzo Mulazzani, Adrian Saftoiu, Zeno Sparchez, Ioan Sporea, Christoph F Dietrich
AIM: To analyze contrast-enhanced ultrasound (CEUS) features of histologically proven hepatic epithelioid hemangioendothelioma (HEHE) in comparison to other multilocular benign focal liver lesions (FLL). METHODS: Twenty-five patients with histologically proven HEHE and 45 patients with histologically proven multilocular benign FLL were retrospectively reviewed. Four radiologists assessed the CEUS enhancement pattern in consensus. RESULTS: HEHE manifested as a single (n = 3) or multinodular (n = 22) FLL...
May 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27064738/discovery-characterization-and-functional-study-of-a-novel-mef2d-cag-repeat-in-duck-anas-platyrhynchos
#16
Yushi Wang, Jiwen Wang, Hehe Liu, Rongping Zhang, Tao Zhang, Xiang Gan, Huilan Huang, Da Chen, Liang Li
Myocyte enhancer transcription factor 2D (MEF2D) is an important transcription factor for promoting the growth and development of muscle. CAG repeats have been found in the coding sequence (CDS) of avian MEF2D; however, their functions remain unknown and require further investigation. Here, we examined the characteristics and functional role of MEF2D CAG repeat in duck. The full-length CDS of duck MEF2D was cloned for the first time, and a novel CAG repeat was identified and located in exon 9. Sequence analysis indicated that the protein domains of duck MEF2D are highly conserved relative to other vertebrates, whereas MEF2D CAG repeats with variable repeat numbers are specific to avian species...
August 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27040525/characterization-of-the-duck-anas-platyrhynchos-rbm24-and-rbm38-genes-and-their-expression-profiles-in-myoblast-and-skeletal-muscle-tissues
#17
Wenqiang Sun, Yaodong Hu, Hengyong Xu, Hua He, Chunchun Han, Hehe Liu, Jiwen Wang, Liang Li
RNA-binding motif proteins 24 (Rbm24) and 38 (Rbm38) are known to regulate genes expression in a post-transcriptional way. However, it remains unclear about the similarities and differences between Rbm24 and Rbm38 in terms of their sequence characteristics and expression profiles during myoblast differentiation and skeletal muscle development. In this study, we found that the coding domain sequences (CDSs) of duck Rbm24 and Rbm38 consisted of 678 and 648 nucleotides, respectively. Both of them contain a conserved RNA-recognition motif (RRM)...
August 2016: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27007909/six1-induces-protein-synthesis-signaling-expression-in-duck-myoblasts-mainly-via-up-regulation-of-mtor
#18
Haohan Wang, Xinxin Li, Hehe Liu, Lingli Sun, Rongping Zhang, Liang Li, Mincheng Wangding, Jiwen Wang
As a critical transcription factor, Six1 plays an important role in the regulation of myogenesis and muscle development. However, little is known about its regulatory mechanism associated with muscular protein synthesis. The objective of this study was to investigate the effects of overexpression ofSix1 on the expression of key protein metabolism-related genes in duck myoblasts. Through an experimental model where duck myoblasts were transfected with a pEGFP-duSix1 construct, we found that overexpression of duckSix1 could enhance cell proliferation activity and increase mRNA expression levels of key genes involved in the PI3K/Akt/mTOR signaling pathway, while the expression of FOXO1, MuRF1and MAFbx was not significantly altered, indicating thatSix1 could promote protein synthesis in myoblasts through up-regulating the expression of several related genes...
March 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/26946613/effect-of-compound-kushen-injection-on-t-cell-subgroups-and-natural-killer-cells-in-patients-with-locally-advanced-non-small-cell-lung-cancer-treated-with-concomitant-radiochemotherapy
#19
RANDOMIZED CONTROLLED TRIAL
Zhongquan Zhao, Hehe Liao, Ying Ju
OBJECTIVE: To observe effect of compound Kushen injection on T-cell subgroups and NK cells in patients with locally advanced non-small-cell lung cancer (NSCLC) treated with concomitant radiochemotherapy. METHODS: We randomly divided 60 patients with locally advanced NSCLC who were treated at our hospital between May 2011 and May 2013 into a treatment group and a control group by drawing. The treatment group (n = 30) received concomitant radiochemotherapy plus compound Keshen injection, and the control group (n = 30) received only radiochemotherapy...
February 2016: Journal of Traditional Chinese Medicine, Chung i Tsa Chih Ying Wen Pan
https://www.readbyqxmd.com/read/26845041/insulin-stimulates-goose-liver-cell-growth-by-activating-pi3k-akt-mtor-signal-pathway
#20
Chunchun Han, Shouhai Wei, Qi Song, Fang He, Xiangping Xiong, Huofu Wan, Dandan Liu, Fengjiang Ye, Hehe Liu, Liang Li, Hongyong Xu, Xiaohui Du, Bo Kang, Xianyin Zeng
BACKGROUND/AIMS: Recent studies have suggested a crucial role for PI3K-Akt-mTOR pathway in regulating cell proliferation, so we hypothesize that insulin acts goose hepatocellular growth by PI3K-Akt-mTOR signal pathway. Because the physiological status of liver cells in vitro is different from that in vivo, a simplified cell model in vitro was established. METHODS: Goose primary hepatocytes were isolated and incubated in either no addition as a control or insulin or PI3K-Akt-mTOR pathway inhibitors or co-treatment with glucose and PI3K-Akt-mTOR pathway inhibitors; Then, cell DNA synthesis and cell cycle analysis were detected by BrdU-incorporation Assay and Flow cytometric analysis; the mRNA expression and protein expression of factors involved in the cell cycle were determined by Real-Time RT-PCR, ELISA, and western blot...
2016: Cellular Physiology and Biochemistry
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