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https://www.readbyqxmd.com/read/27925005/seed-desiccation-tolerance-and-cryopreservation-of-philippine-calamansi-x-citrofortunella-microcarpa-bunge-wijnands
#1
O L Damasco, L C Refuerzo
BACKGROUND: The traditional on farm conservation of Calamansi [x Citrofortunella microcarpa (Bunge) Wijnands], an important indigenous Citrus species in the Philippines, is now being threatened by shifting agricultural crop production, climate change, and increasing biotic and abiotic stresses. OBJECTIVE: The study aimed to characterize the desiccation and cryopreservation tolerance of seeds as the basis for complementary long term ex situ conservation. MATERIALS AND METHODS: Intact seeds were desiccated in an airtight container filled with activated silica gel for 0-96 h...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924934/one-pot-synthesis-of-class-ii-lanthipeptide-bovicin-hj50-via-an-engineered-lanthipeptide-synthetase
#2
Jian Wang, Xiaoxuan Ge, Li Zhang, Kunling Teng, Jin Zhong
Lanthipeptides are a large class of bacteria-produced, ribosomally-synthesized and post-translationally modified peptides. They are recognized as peptide antibiotics because most of them exhibit potent antimicrobial activities against Gram-positive bacteria especially those that are phylogenetically related to producers. Maturation of class II lanthipeptide like bovicin HJ50 undergoes precursor modification by LanM and a subsequent leader peptide cleavage by LanT. Herein, via co-expression of precursor gene bovA, modification gene bovM and transporter gene bovT in Escherichia coli C43 (DE3), bioactive bovicin HJ50 was successfully produced and secreted...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924781/development-of-sns2-rgo-nanosheet-composite-for-cost-effective-aqueous-hybrid-supercapacitors
#3
Himani Chauhan, Manoj K Singh, Praveen Kumar, Safir Ahmad Hashmi, Sasanka Deka
The development of low cost supercapacitor cells with unique capacitive properties is essential for many domestic and industrial purposes. Here we report the first ever application of SnS2-reduced graphene oxide (SnS2/RGO) layered nanocomposite as a superior electrode material for symmetric aqueous hybrid supercapacitors. We synthesized SnS2/RGO nanocomposite comprised of nanosheets of SnS2 and graphene oxide via a one-pot hydrothermal approach. in situ as-synthesized SnS2/RGO is devised for the first time to give high specific capacitance 500 Fg(-1), energy density 16...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27924345/chemoenzymatic-synthesis-of-para-nitrophenol-pnp-tagged-%C3%AE-2-8-sialosides-and-high-throughput-substrate-specificity-studies-of-%C3%AE-2-8-sialidases
#4
Nova Tasnima, Hai Yu, Yanhong Li, Abhishek Santra, Xi Chen
para-Nitrophenol (pNP)-tagged α2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification...
December 7, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27923155/user-preferences-and-willingness-to-pay-for-safe-drinking-water-experimental-evidence-from-rural-tanzania
#5
Zachary Burt, Robert M Njee, Yolanda Mbatia, Veritas Msimbe, Joe Brown, Thomas F Clasen, Hamisi M Malebo, Isha Ray
Almost half of all deaths from drinking microbiologically unsafe water occur in Sub-Saharan Africa. Household water treatment and safe storage (HWTS) systems, when consistently used, can provide safer drinking water and improve health. Social marketing to increase adoption and use of HWTS depends both on the prices of and preferences for these systems. This study included 556 households from rural Tanzania across two low-income districts with low-quality water sources. Over 9 months in 2012 and 2013, we experimentally evaluated consumer preferences for six "low-cost" HWTS options, including boiling, through an ordinal ranking protocol...
November 25, 2016: Social Science & Medicine
https://www.readbyqxmd.com/read/27922205/one-pot-preparation-of-dimethyl-isosorbide-from-d-sorbitol-via-dimethyl-carbonate-chemistry
#6
Fabio Arico', Pietro Tundo, Alexander Aldoshin
Direct synthesis of dimethyl isosorbide (DMI) from D-sorbitol via dimethyl carbonate (DMC) chemistry is herein firstly reported. High yield of DMI was achieved using nitrogen superbase 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD) as catalyst and performing the reaction in a stainless steel autoclave by increasing the temperature from 90°C to 200 °C. In this procedure DMC features its full capacity, acting in the different steps of the process as carboxymethylating, leaving group (cyclization) and methylating agent; DMC is also employed as the reaction media...
December 6, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27919691/one-pot-synthesis-of-glutathione-by-a-two-enzyme-cascade-using-a-thermophilic-atp-regeneration-system
#7
Xing Zhang, Hui Wu, Bing Huang, Zhimin Li, Qin Ye
In vitro cascade catalysis using enzyme-based system is becoming a promising biomanufacturing platform for biofuels and biochemicals production. Glutathione is a pivotal non-protein thiol compound and widely applied in food and pharmaceutical industries. In this study, glutathione was synthesized by a bifunctional glutathione synthetase together with a thermophilic ATP regeneration system through a two-enzyme cascade in vitro. Four bifunctional glutathione synthetases from Streptococcus sanguinis, S. gordonii, S...
December 2, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27918986/hybrid-chitosan-polyaniline-polypyrrole-biomaterial-for-enhanced-adsorption-and-antimicrobial-activity
#8
Rajeev Kumar, Mohammad Oves, Talla Ameelbi, Naief H Al-Makishah, M A Barakat
In this work, chitosan (CS) functionalized polyaniline-polypyrrole (Pani-Ppy) copolymer (CS/Pani-Ppy) was synthesized applying a facile one pot method for the enhanced adsorption of Zn(II) and antimicrobial activity for E. coli and E. agglomerans. The synthesized materials were characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform inferred spectroscopy and X-ray photoelectron spectroscopy. The adsorption of the Zn(II) on the synthesized materials was highly dependent on the pH of the solution, the initial metal ion concentration, and temperature...
November 24, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27918648/functionalized-natural-carbon-supported-nanoparticles-as-excellent-catalysts-for-hydrocarbon-production
#9
Jian Sun, Lisheng Guo, Qingxiang Ma, Xinhua Gao, Noriyuki Yamane, Hengyong Xu, Noritatsu Tsubaki
We report a one-pot and eco-friendly synthesis of carbon supported cobalt nanoparticles, achieved by carbonization of waste biomass (rice bran) with a cobalt source. The functionalized biomass provides carbon microspheres as excellent catalyst support, forming a unique interface between hydrophobic and hydrophilic groups. The latter, involving hydroxyl and amino groups, can catch much more active cobalt nanoparticles on surface for Fischer-Tropsch synthesis than chemical carbon. The loading amount of cobalt on the final catalyst is much higher than that prepared with a chemical carbon source, like glucose...
December 5, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27918637/intermolecular-aryl-c-h-amination-using-sequential-iron-and-copper-catalysis
#10
Mohamed Mostafa, Ewen Calder, Daugirdas Racys, Andrew Sutherland
A mild, efficient and regioselective method for para-amination of activated arenes has been developed using a combination of iron and copper catalysis. A diverse range of products were obtained from an operationally simple one-pot, two-step procedure involving bromination of the aryl substrate using the powerful Lewis acid iron(III) triflimide, followed by a copper(I)-catalyzed N-arylation reaction. This two-step dehydrogenative process for the regioselective coupling of aromatic C-H bonds with nonactivated amines was applicable to anisole-, phenol-, aniline- and acetanilide-type aryl compounds...
December 5, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27918127/iron-catalyzed-oxidative-c-c-and-n-n-coupling-of-diarylamines-and-synthesis-of-spiroacridines
#11
Raphael F Fritsche, Gabriele Theumer, Olga Kataeva, Hans-Joachim Knölker
We describe iron-catalyzed intermolecular oxidative coupling reactions of diarylamines to form substituted 2,2'-bis(arylamino)biaryl compounds, tetraarylhydrazines, and 5,6-dihydrobenzo[c]cinnolines with the same hexadecafluorinated iron-phthalocyanine catalyst. The mild formation of C-C or N-N bonds was controlled by the use of acidic or basic additives. In contrast to most iron-catalyzed dehydrogenative coupling reactions, ambient air could be used as the sole oxidant. Moreover, iron(III) chloride hexahydrate promoted a one-pot coupling and subsequent intramolecular dearomative coupling to give 10H-spiro[acridine-9,1'-cyclohexa-2',5'-dien-4'-ones]...
December 5, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27917720/ultrasound-promoted-synthesis-and-anticoagulant-activity-of-2-amino-4h-chromen-4-ylphosphonates
#12
Sarva Santhisudha, Tellamekala Sreekanth, Sudileti Murali, Bathal Vijaya Kumar, Meruva Anila Devi, Cirandur Suresh Reddy
BACKGROUND: One of the alarming difficulties in the field of hematology is the Coagulant disorders. Despite the availability of clinically proven existing anticoagulants, their limitations have prompted a continuous search for novel anticoagulants. OBJECTIVE: The primary objective of the study is to synthesize a series of 2-amino-4H-chromen-4-ylphosphonate derivatives and to test their anticoagulant activity depending on PT measurements con-sidering commercial heparin as positive control...
December 4, 2016: Cardiovascular & Hematological Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/27917427/sulfoxidation-of-alkenes-and-alkynes-with-nfsi-as-a-radical-initiator-and-selective-oxidant
#13
Yuexia Zhang, Zeng Rong Wong, Xingxing Wu, Sherman J L Lauw, Xuan Huang, Richard D Webster, Yonggui Robin Chi
Sulfoxides are important functional molecules. We develop a short-route (one-pot) synthesis of this class of molecules by reacting thiols with alkenes or alkynes under mild and metal-free conditions. N-Fluorobenzenesulfonimide (NFSI) is used to play dual roles: as a radical initiator for a thiol-ene/-yne reaction to form sulfide adducts, and as efficient oxidant for conversion of the sulfides formed in situ to sulfoxides. Over-oxidation of the sulfoxides to sulfones is avoided in our approach.
December 5, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27917420/a-dna-kinetics-competition-strategy-of-hybridization-chain-reaction-for-molecular-information-processing-circuit-construction
#14
Weiyang Tang, Qichen Huang, Zhenjie Yang, Qiwei Zheng, Lihong Wang, Junmin Zhang, Liang Chen, Xuechang Zhou, Yizhen Liu, Jiming Hu
Herein we propose a hybridization chain reaction based kinetics competition strategy able to recognize the combination/sequence of input signals. Further, a DNA priority encoder and a keypad lock were constructed. This strategy allowed us to develop a one-pot assay that can automatically and smartly respond to multiple inputs.
December 5, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27917017/determination-of-coefficient-defining-leaf-area-development-in-different-genotypes-plant-types-and-planting-densities-in-peanut-arachis-hypogeae-l
#15
Oumarou Halilou, Halime Mahamat Hissene, José A Clavijo Michelangeli, Falalou Hamidou, Thomas R Sinclair, Afshin Soltani, Saadou Mahamane, Vincent Vadez
Rapid leaf area development may be attractive under a number of cropping conditions to enhance the vigor of crop establishment and allow rapid canopy closure for maximizing light interception and shading of weed competitors. This study was undertaken to determine (1) if parameters describing leaf area development varied among ten peanut (Arachis hypogeae L.) genotypes grown in field and pot experiments, (2) if these parameters were affected by the planting density, and (3) if these parameters varied between Spanish and Virginia genotypes...
December 2016: Field Crops Research
https://www.readbyqxmd.com/read/27916534/critical-role-of-the-proton-dependent-oligopeptide-transporter-pot-in-the-cellular-uptake-of-the-peptidyl-nucleoside-antibiotic-blasticidin-s
#16
Kenji Kitamura, Eldaa Zefany Banami Kinsui, Fumiyoshi Abe
Blasticidin S (BlaS) interferes in the cell growth of both eukaryotes and prokaryotes. Its mode of action as a protein synthesis inhibitor has been investigated extensively. However, the mechanism of BlaS transport into the target cells is not understood well. Here, we show that Ptr2, a member of the proton-dependent oligopeptide transporter (POT) family, is responsible for the uptake of BlaS in yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. Notably, some mutants of Ptr2 that are dysfunctional in dipeptide uptake were still competent to transport BlaS...
December 1, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27915180/self-primed-isothermal-amplification-for-genomic-dna-detection-of-human-papillomavirus
#17
Wei Lu, Qingpan Yuan, Zhiliu Yang, Bo Yao
Rolling circle amplification (RCA) is an isothermal amplification technique with high efficiency and perfect accuracy for nucleic acids detection. However, RCA technique suffers the limitation to detect short DNA or RNA molecules. For long nucleic acid molecules, enzymatic restriction as well as heat denaturation process is usually required, which makes the amplification not effective and strictly isothermal. In this article, a simple and efficient one-pot self-primed isothermal amplification (SIA) was developed for detection of genomic DNA directly based on the combination of nicking endonuclease assisted strand displacement amplification (SDA) and exponential RCA...
October 10, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27915173/effect-of-prolonged-water-stress-on-essential-oil-content-compositions-and-gene-expression-patterns-of-mono-and-sesquiterpene-synthesis-in-two-oregano-origanum-vulgare-l-subspecies
#18
Mohammad Reza Morshedloo, Lyle E Craker, Alireza Salami, Vahideh Nazeri, Hyunkyu Sang, Filippo Maggi
Origanum vulgare L., recognized throughout the world as a popular medicinal and flavoring herb, contains a wide array of medicinally active components, including phenolic glucosides, flavonoids, tannins, sterols and high amounts of terpenoids. Especially the latter are often extracted by hydrodistillation resulting in the so-called essential oil that is rich in monoterpenes (e.g. carvacrol, thymol, linalyl acetate) and/or sesquiterpenes (e.g. (E)-β-caryophyllene, germacrene D, bicyclogermacrene, β-caryophyllene oxide)...
November 28, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27915144/antioxidant-enzyme-and-osmotic-adjustment-changes-in-bean-seedlings-as-affected-by-biochar-under-salt-stress
#19
Salar Farhangi-Abriz, Shahram Torabian
Salinity damaged cellular membranes through overproduction of reactive oxygen species (ROS), while osmolytes and antioxidant capacities play a vital role in protecting plants from salinity caused oxidative damages. Biochar also could alleviate the negative impacts of salt stress in crops. The pot experiment was conducted to investigate the effects of biochar on some antioxidant enzyme activities and osmolyte adjustments of common bean (Phaseolus vulgaris L. cv. Derakhshan) under salinity stress. Bean plants were subjected to three salinity levels (non-saline, 6 and 12 dSm(-1) of NaCl) and biochar treatments (non-biochar, 10% and 20% total pot mass)...
December 1, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27915028/catalytic-synthesis-of-%C3%AE-amino-chromone-phosphonates-and-their-antimicrobial-toxicity-and-potential-hiv-1-rt-inhibitors-based-on-silico-screening
#20
Abosede Oluwabukola Jaiyeola, K Anand, K Kasumbwe, M Ramesh, R M Gengan
The α-amino phosphonates exhibit a wide range of biological properties which create demand for simple and efficient synthetic routes of new molecules. In this study synthesis of a new series of derivatives of α-amino chromone phosphonates (ACPs) was reported using RhBT catalyst. The RhBT was first prepared by simply mixing boron nitride in a solution of rhodium acetate, under inert atmosphere for a week followed by filtration (yield: 92%). The catalyst can be re-used for up to four times and showed minimal loss of activity...
November 23, 2016: Journal of Photochemistry and Photobiology. B, Biology
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