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https://www.readbyqxmd.com/read/29750515/dimerization-through-the-ring-finger-domain-attenuates-excision-activity-of-the-piggybac-transposase
#1
Rahul Sharma, Shivlee Nirwal, Naveen Narayanan, Deepak T Nair
The movement of the piggyBac transposon is mediated through its cognate transposase. The piggyBac transposase binds to the terminal repeats present at the ends of the transposon. This is followed by excision of the transposon and release of the nucleoprotein complex. The complex translocates, followed by integration of the transposon at the target site. Here, we show that the RING-finger domain (RFD) present toward the C-terminus of the transposase is vital for dimerization of this enzyme. The deletion of the RFD or the last seven residues of the RFD results in a monomeric protein that binds the terminal end of the transposon with nearly the same affinity as wild type piggyBac transposase...
May 11, 2018: Biochemistry
https://www.readbyqxmd.com/read/29727405/nitrate-supplement-benefits-contractile-forces-in-fatigued-but-not-unfatigued-muscle
#2
Neale A Tillin, Sarah Moudy, Kirsty M Nourse, Christopher J Tyler
PURPOSE: Evidence suggests dietary nitrate supplementation enhances low-frequency (≤20 Hz) involuntary, but not voluntary, forces in unfatigued human muscle. We investigated the hypotheses that nitrate supplementation would also attenuate low-frequency fatigue and the loss of explosive-voluntary forces in fatigued conditions. METHODS: In a counterbalanced double-blinded order, 17 male participants completed two experimental trials following 7 days of dietary supplementation with either nitrate-rich (NIT) or nitrate-depleted (PLA) beetroot juice...
April 27, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29707792/maintenance-of-pre-existing-dna-methylation-states-through-recurring-excess-light-stress
#3
Diep R Ganguly, Peter A Crisp, Steven R Eichten, Barry J Pogson
The capacity for plant stress priming and memory, and the notion of this being underpinned by DNA methylation-mediated memory is an appealing hypothesis for which there is mixed evidence. We previously established a lack of drought-induced methylome variation in Arabidopsis thaliana (Arabidopsis); however, this was tied to only minor observations of physiological memory. There are numerous independent observations demonstrating that photoprotective mechanisms, induced by excess-light (EL) stress, can lead to robust programmable changes in newly developing leaf tissues...
April 29, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29629467/currently-used-organophosphate-flame-retardants-determined-in-the-settled-dust-of-masjids-and-hotels-of-saudi-arabia-a-new-insight-into-human-health-implications-of-dust-exposure
#4
Nadeem Ali, Iqbal Mohammad Ibrahim Ismail, Mohammad W Kadi, Hussain Mohammed Salem Ali Albar
Indoor settled dust particles are considered as an important source of human exposure to chemicals such as organophosphate flame retardants (PFRs). In recent decades the Kingdom of Saudi Arabia (KSA) has experienced tremendous growth in population, as a result the number of masjids has also increased significantly to provide sufficient space for the public to offer prayers. The hospitality industry in KSA is also expanding to cater for the ever-increasing number of pilgrims visiting the two holy cities of the kingdom...
April 9, 2018: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/29621883/sentinel-wraps-real-time-monitoring-of-food-contamination-by-printing-dnazyme-probes-on-food-packaging
#5
Hanie Yousefi, M Monsur Ali, Hsuan-Ming Su, Carlos D M Filipe, Tohid F Didar
Here, we report the development of a transparent, durable, and flexible sensing surface that generates a fluorescence signal in the presence of a specific target bacterium. This material can be used in packaging, and it is capable of monitoring microbial contamination in various types of food products in real time without having to remove the sample or the sensor from the package. The sensor was fabricated by covalently attaching picoliter-sized microarrays of an E. coli-specific RNA-cleaving fluorogenic DNAzyme probe (RFD-EC1) to a thin, flexible, and transparent cyclo-olefin polymer (COP) film...
April 6, 2018: ACS Nano
https://www.readbyqxmd.com/read/29553911/polychlorobiphenyl-levels-in-the-serum-of-cats-from-residential-flats-in-italy-role-of-the-indoor-environment
#6
Francesco Paolo Serpe, Filomena Fiorito, Mauro Esposito, Angelo Ferrari, Federico Fracassi, Roberto Miniero, Marco Pietra, Paola Roncada, Gianfranco Brambilla
The domestic cat (Felis catus) was used as a sentinel of exposure to polychlorobiphenyls (PCBs) in indoor urban environments y. Sera from 120 cats were pooled to form 30 different groups selected by age (<2 years; > 2 ≤ 8 years; > 8 years), sex, municipality (Bologna and Turin) and environment (indoor vs. outdoor). Test portions of 1 mL were analyzed by means of gas chromatography coupled to high-resolution mass spectrometry (GC-HRMS) for six selected indicators non-dioxin-like PCBs (∑6 PCBs: congeners #28, #52, #101, #132, #153 and #180) and the results were computed in the upper-bound mode...
March 19, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29547490/within-and-between-session-reliability-of-the-isometric-mid-thigh-pull-in-young-female-athletes
#7
Sylvia Moeskops, Jon L Oliver, Paul J Read, John B Cronin, Gregory D Myer, G Gregory Haff, Rhodri S Lloyd
To investigate the within- and between-session reliability of the isometric mid-thigh pull (IMTP) in pre- and post-peak height velocity (PHV) female athletes. Nineteen pre- and nineteen post-PHV athletes performed bilateral IMTPs using a custom-designed isometric testing system. Participants attended three separate testing-sessions, and performed three trials within each session. Peak force, relative peak force, force at 30, 50, 90, 100, 150, 200, and 250 milliseconds, rate of force development (RFD) within time-specific bands, time to peak force (TPF), and time to peak rate of force development (TPRFD) were obtained for analysis...
March 15, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29541137/the-improvement-of-suspension-training-for-trunk-muscle-power-in-sanda-athletes
#8
Xiujie Ma, Wei Sun, An Lu, Pei Ma, Chuanyin Jiang
The aim of this study was to investigate whether both suspension training (ST) and traditional training (TT) can improve Sanda athlete's strength quality of trunk muscles and to explore the effect of suspension training on Sanda athletes' trunk muscle power production. Twelve elite Sanda athletes from the Competitive Sports School of Shanghai University of Sport were randomly assigned to experimental group (EG) and control group (CG). EG and CG were regularly trained with suspension training and traditional strength training for 40 minutes three times per week...
December 2017: Journal of Exercise Science and Fitness
https://www.readbyqxmd.com/read/29533358/kinetic-analysis-of-horizontal-plyometric-exercise-intensity
#9
Andrew J Kossow, William P Ebben
Kossow, AJ, DeChiara, TG, Neahous, SM, and Ebben, WP. Kinetic analysis of horizontal plyometric exercise intensity. J Strength Cond Res 32(5): 1222-1229, 2018-Plyometric exercises are frequently performed as part of a strength and conditioning program. Most studies assessed the kinetics of plyometric exercises primarily performed in the vertical plane. The purpose of this study was to evaluate the multiplanar kinetic characteristics of a variety of plyometric exercises, which have a significant horizontal component...
May 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29511921/increased-rate-of-force-development-during-periodized-maximum-strength-and-power-training-is-highly-individual
#10
Heikki Peltonen, Simon Walker, Anthony C Hackney, Janne Avela, Keijo Häkkinen
Maximum strength training induces various improvements in the rate of force development (RFD) on a group level, but no study has investigated inter-individual adaptations in RFD. Fourteen men (28 ± 6 years old) performed the same 10-week maximum strength and then a 10-week power training program. Maximal force and RFD were recorded during maximal isometric leg extension voluntary contractions repeatedly before every 7th training session (2 sessions/week). After the intervention, subjects were retrospectively divided into three groups based on their RFD improvements: (1) improved only during the maximum strength period (MS-responders, + 100 ± 35%), (2) improved only during the power period (P-responders, + 53 ± 27%) or (3) no improvement at all (non-responders, + 3 ± 9%)...
March 6, 2018: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29503581/neck-shoulder-function-in-tension-type-headache-patients-and-the-effect-of-strength-training
#11
Bjarne K Madsen, Karen Søgaard, Lars L Andersen, Jørgen Skotte, Birte Tornøe, Rigmor H Jensen
Introduction: Muscle pain has been associated with reduced maximal muscle strength, and reduced rate of force development (RFD). Strength training (ST) has shown an effect in not only normalizing muscle function but also reducing neck muscle pain. Aim: The aims of this study were to compare muscle function in terms of strength, force steadiness in neck flexion, as well as extension, and rate of RFD of the shoulder in tension-type headache (TTH) patients and healthy controls and to examine the correlation to tenderness...
2018: Journal of Pain Research
https://www.readbyqxmd.com/read/29490592/women-show-similar-central-and-peripheral-fatigue-to-men-after-half-marathon
#12
Gennaro Boccia, Davide Dardanello, Cantor Tarperi, Luca Festa, Antonio La Torre, Barbara Pellegrini, Federico Schena, Alberto Rainoldi
Women are known to be less fatigable than men in single-joint exercises, but fatigue induced by running has not been well understood. Here we investigated sex differences in central and peripheral fatigue and in rate of force development (RFD) in the knee extensors after a half-marathon run. Ten male and eight female amateur runners (aged 25-50 years) were evaluated before and immediately after a half-marathon race. Knee extensors forces were obtained under voluntary and electrically evoked isometric contractions...
March 1, 2018: European Journal of Sport Science
https://www.readbyqxmd.com/read/29484358/high-intensity-multimodal-resistance-training-improves-muscle-function-symmetry-during-a-sit-to-stand-task-and-physical-function-following-hip-fracture
#13
R A Briggs, J R Houck, P C LaStayo, J M Fritz, M J Drummond, R L Marcus
OBJECTIVES: Post rehabilitation, older adults with hip fracture display low vertical ground reaction force (vGRF) on the involved lower extremity during a sit-to-stand task and low physical function. The purpose of this study was to test whether muscle performance, involved side vGRF during a sit-to-stand task, and physical function improved following multimodal high-intensity resistance training, when initiated after usual care (2 to 6 months after hip fracture). DESIGN: Case series study, 12 weeks extended high-intensity strength training intervention following hip fracture...
2018: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/29474465/strength-improvements-through-occlusal-splints-the-effects-of-different-lower-jaw-positions-on-maximal-isometric-force-production-and-performance-in-different-jumping-types
#14
Christian Maurer, Sebastian Heller, Jil-Julia Sure, Daniel Fuchs, Christoph Mickel, Eileen M Wanke, David A Groneberg, Daniela Ohlendorf
OBJECTIVE: The influence of the jaw position on postural control, body posture, walking and running pattern has been reported in the literature. All these movements have in common that a relatively small, but well controlled muscle activation is required. The induced effects on motor output through changed jaw positions have been small. Therefore, it has been questioned if it could still be observed in maximal muscle activation. METHOD: Twenty-three healthy, mid age recreational runners (mean age = 34...
2018: PloS One
https://www.readbyqxmd.com/read/29462969/milk-an-effective-recovery-drink-for-female-athletes
#15
Paula Rankin, Adrian Landy, Emma Stevenson, Emma Cockburn
Milk has become a popular post-exercise recovery drink. Yet the evidence for its use in this regard comes from a limited number of investigations utilising very specific exercise protocols, and mostly with male participants. Therefore, the aim of this study was to investigate the effects of post-exercise milk consumption on recovery from a sprinting and jumping protocol in female team-sport athletes. Eighteen females participated in an independent-groups design. Upon completion of the protocol participants consumed 500 mL of milk (MILK) or 500 mL of an energy-matched carbohydrate (CHO) drink...
February 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29444538/-effects-of-judo-specific-resistance-training-on-kinetic-and-electromyographic-parameters-of-pulling-exercises-in-judo-athletes
#16
Norman Helm, Olaf Prieske, Thomas Muehlbauer, Tom Krüger, Urs Granacher
BACKGROUND:  In judo, rapid force production during pulling movements is an important component of athletic performance, which is why this capacity needs to be specifically exercised in judo. This study aimed at examining the effects of a judo-specific resistance training program using a judo ergometer system (PTJ) versus a traditional resistance training regime using a partner (PTP) on kinetics and muscle activity of judo-specific pulling exercises. METHOD:  Twenty-four male judo athletes (age: 22 ± 4 years, training experience: 15 ± 3 years) were randomly assigned to two groups...
February 14, 2018: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
https://www.readbyqxmd.com/read/29432049/polybrominated-diphenyl-ethers-pbdes-in-car-and-house-dust-from-thailand-implication-for-human-exposure
#17
Dudsadee Muenhor, Stuart Harrad
This study examines concentrations of tri- to hexa-bromodiphenylethers (BDEs 17, 28, 47, 49, 66, 85, 99, 100, 153 and 154; Σ10 PBDEs (polybrominated diphenyl ethers)) in car (n = 30) and house dust samples (n = 53) collected from different provinces in Thailand. The specific objectives of this study were: (i) to monitor levels of tri- to hexa-bromodiphenylethers (BDEs 17, 28, 47, 49, 66, 85, 99, 100, 153 and 154; Σ10 PBDEs) in vehicle and residential dust sampled from various provinces in Thailand; (ii) to compare PBDE concentrations with those detected for domestic and automobile dust in studies from other countries and (iii) to estimate exposure of the Thai population to the target PBDEs via dust ingestion...
February 12, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29412787/measurement-of-respiratory-function-decline-in-patients-with-duchenne-muscular-dystrophy-a-conjoint-analysis
#18
Elena Ripamonti, Grazia D'Angelo
AIM: In Duchenne muscular dystrophy (DMD), little attention has been paid to severity of respiratory function decline (RFD) based on disease progression. We performed a conjoint analysis among 123 Italian clinicians to generate a scale for RFD in DMD patients. METHODS: Before the interview, 11 attributes were selected by discussion among experts. 32 'patient profiles' were generated. Each physician assessed the severity of RFD for each profile. Each level/attribute was assigned an estimated usefulness to understand its impact on RFD...
April 2018: Neurodegenerative Disease Management
https://www.readbyqxmd.com/read/29408538/lower-quadriceps-rate-of-force-development-is-associated-with-worsening-physical-function-in-adults-with-or-at-risk-for-knee-osteoarthritis-36-month-follow-up-data-from-the-osteoarthritis-initiative
#19
Bo Hu, Søren Thorgaard Skou, Barton L Wise, Glenn N Williams, Michael C Nevitt, Neil A Segal
OBJECTIVE: To determine the association between quadriceps rate of force development (RFD) and decline in self-reported physical function and objective measures of physical performance. DESIGN: Longitudinal cohort study. SETTING: Community-based sample from 4 urban areas. PARTICIPANTS: Osteoarthritis Initiative participants with or at risk for knee osteoarthritis, who had no history of knee/hip replacement, knee injury, or rheumatoid arthritis (N=2630)...
January 31, 2018: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29387636/the-time-has-come-to-incorporate-a-greater-focus-on-rate-of-force-development-training-in-the-sports-injury-rehabilitation-process
#20
REVIEW
Matthew Buckthorpe, Giulio Sergio Roi
This narrative and literature review discusses the relevance of Rate of Force Development (RFD) (the slope of the force time curve) for Return To Sport (RTS), its determinants and the influence of training practices on it expression, with the purpose to enhance clinicians' awareness of how RFD training may enhance RTS success. RFD is considered functionally more relevant than maximal muscle strength during certain very fast actions including rapid joint stabilisation following mechanical perturbation. Deficits in RFD are reported following conventional rehabilitation programmes despite full restoration of maximal strength, which may contribute to the less than satisfactory RTS outcomes reported in the literature...
July 2017: Muscles, Ligaments and Tendons Journal
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