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https://www.readbyqxmd.com/read/28541714/a-multivariate-evaluation-of-factors-affecting-the-quality-of-freshly-frozen-tissue-specimens
#1
Tong-Hong Wang, Chin-Chuan Chen, Kung-Hao Liang, Chi-Yuan Chen, Wen-Yu Chuang, Shir-Hwa Ueng, Pao-Hsien Chu, Chung-Guei Huang, Tse-Ching Chen, Chuen Hsueh
Well-prepared and preserved freshly frozen specimens are indispensable materials for clinical studies. To manage specimen quality and to understand the factors potentially affecting specimen quality during preservation processes, we analyzed the quality of RNA and genomic DNA of various tissues collected between 2002 and 2011 in Linkou Chang Gung Memorial Hospital, Taiwan. During this period, a total of 1059 freshly frozen specimens from eight major cancer categories were examined. It was found that preservation duration, organ origin, and tissue type could all influence the quality of RNA samples...
May 25, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28539271/gene-expression-profiling-of-tumor-associated-macrophages-after-exposure-to-single-dose-irradiation
#2
Wei-Hsiang Kung, Ching-Fang Yu, Andy Chi-Lung Lee, Chi-Dung Yang, Yu-Chen Liu, Fang-Hsin Chen, Hsien-Da Huang
Radiotherapy (RT) is a common cancer treatment approach that accounts for nearly 50% of patient treatment; however, tumor relapse after radiotherapy is still a major issue. To study the crucial role of tumor-associated macrophages (TAMs) in the regulation of tumor progression post-RT, microarray experiments comparing TAM gene expression profiles between unirradiated and irradiated tumors were conducted to discover possible roles of TAMs in initiation or contribution to tumor recurrence following RT, taking into account the relationships among gene expression, tumor microenvironment, and immunology...
April 30, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28497504/semiconducting-coordination-polymers-based-on-the-pre-designed-ternary-te-fe-cu-carbonyl-cluster-and-conjugation-interrupted-dipyridyl-linkers
#3
Minghuey Shieh, Chia-Chi Yu, Chia-Yeh Miu, Chang-Hung Kung, Chung-Yi Huang, Yu-Hsin Liu, Hsiang-Lin Liu, Chih-Chiang Shen
A series of semiconducting cluster-incorporated Cu-based coordination polymers, namely, 1D zigzag polymers [[{TeFe3(CO)9Cu2}(L)]n (L = 1,2-bis(4-pyridyl)ethane (bpea), 1; L = 1,2-bis(4-pyridyl)ethylene (bpee), 5), 2D honeycomb-like polymers [{TeFe3(CO)9Cu}Cu(L)2.5]n (L = bpea, 2; L =bpee, 6), and 2D wave-like cation-anion polymer [{Cu2(L)4}{{TeFe3(CO)9Cu}2(L)}]n (L = 1,3-bis(4-pyridyl)propane (bpp), 4), as well as the macrocycle [{TeFe3(CO)9Cu2}2(bpp)2] (3) have been quantitatively synthesized via the liquid-assisted grinding from the pre-designed cluster [TeFe3(CO)9Cu2(MeCN)2] with conjugated or conjugation-interrupted dipyridyl linkers...
May 11, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28451991/-18-f-fp-dtbz-pet-study-in-a-lactacystin-treated-rat-model-of-parkinson-disease
#4
Chi-Chang Weng, Siao-Lan Huang, Zi-An Chen, Kun-Ju Lin, Ing-Tsung Hsiao, Tzu-Chen Yen, Mei-Ping Kung, Shiaw-Pyng Wey, Ching-Han Hsu
OBJECTIVE: Lactacystin has been used to establish rodent models of Parkinson disease (PD), with cerebral α-synuclein inclusions. This study evaluated the uptake of [(18)F]9-fluoropropyl-(+)-dihydrotetrabenazine ([(18)F]FP-(+)-DTBZ), a vesicular monoamine transporter type 2 (VMAT2)-targeting radiotracer, through positron emission tomography (PET) in lactacystin-treated rat brains. METHODS: Adult male Sprague-Dawley rats were randomly treated with a single intracranial dose of lactacystin (2 or 5 μg) or saline (served as the sham control) into the left medial forebrain bundle...
April 27, 2017: Annals of Nuclear Medicine
https://www.readbyqxmd.com/read/28434461/effect-of-black-tea-consumption-on-radial-blood-pulse-spectrum-and-cognitive-health
#5
Chi-Wei Chang, Sheng-Hung Wang, Ming-Yie Jan, Wei-Kung Wang
Black tea consumption has been proven to improve endothelial function and to lower the risk of stroke and cognitive impairment. Several effects of black tea on cardiovascular system had been surveyed. However, the black tea effect on pressure pulse spectrum remains unknown. The study was aimed to investigate the influence of black tea on radial blood pressure and Pulse Spectrum. Fourteen healthy subjects received water and single doses of black tea (0.05g/Kg) in separate weeks. The radial blood pressure and pulse wave were measured and the pressure pulses were evaluated using harmonic analysis...
April 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28425200/high-dose-calcineurin-inhibitor-free-everolimus-as-a-maintenance-regimen-for-heart-transplantation-may-be-a-risk-factor-for%C3%A2-pneumocystis-pneumonia
#6
Yu-Ning Hu, Nan-Yao Lee, Jun-Neng Roan, Chi-Hsin Hsu, Chwan-Yao Luo
BACKGROUND: Everolimus reduces the incidence of cardiac-allograft vasculopathy (CAV) and is less renally toxic than are calcineurin inhibitors (CNIs). We evaluated the safety of CNI-free everolimus for post-heart transplant (HTx) patients. METHODS: We retrospectively reviewed the records of 36 consecutive patients who had undergone an HTx between January 2006 and December 2013 in National Cheng Kung University Hospital. All patients initially had been treated with the standard tacrolimus regimen...
April 20, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28369084/nmd-classifier-a-reliable-and-systematic-classification-tool-for-nonsense-mediated-decay-events
#7
Min-Kung Hsu, Hsuan-Yu Lin, Feng-Chi Chen
Nonsense-mediated decay (NMD) degrades mRNAs that include premature termination codons to avoid the translation and accumulation of truncated proteins. This mechanism has been found to participate in gene regulation and a wide spectrum of biological processes. However, the evolutionary and regulatory origins of NMD-targeted transcripts (NMDTs) have been less studied, partly because of the complexity in analyzing NMD events. Here we report NMD Classifier, a tool for systematic classification of NMD events for either annotated or de novo assembled transcripts...
2017: PloS One
https://www.readbyqxmd.com/read/28288911/effects-of-traditional-medical-herbs-minor-bupleurum-decoction-on-the-non-specific-immune-responses-of-white-shrimp-litopenaeus-vannamei
#8
Yu-Sheng Wu, Meng-Chou Lee, Cheng-Ting Huang, Tzu-Chi Kung, Chih-Yang Huang, Fan-Hua Nan
This study is investigating the effect of minor bupleurum decoction (Xiao-Chai-Hu decoction) on the non-specific immune response of white shrimp (Litopenaeus vannamei). To determine prophenoloxidase activity (proPO), reactive oxygen species production (ROS), superoxide anion production (O2(-)), nitric oxide production (NO), phagocytic rate (PR), phagocytic index (PI), superoxide dismutase activity (SOD), total haemocyte count (THC) and differential haemocyte count (DHC). In this experiment, treating with different dosages (0, 0...
May 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28257461/quantitative-analysis-of-the-therapeutic-effect-of-magnolol-on-mptp-induced-mouse-model-of-parkinson-s-disease-using-in-vivo-18f-9-fluoropropyl-dihydrotetrabenazine-pet-imaging
#9
Chi-Chang Weng, Zi-An Chen, Ko-Ting Chao, Ting-Wei Ee, Kun-Ju Lin, Ming-Huan Chan, Ing-Tsung Hsiao, Tzu-Chen Yen, Mei-Ping Kung, Ching-Han Hsu, Shiaw-Pyng Wey
18F-9-Fluoropropyl-(+)-dihydrotetrabenazine [18F-FP-(+)-DTBZ] positron emission tomography (PET) has been shown to detect dopaminergic neuron loss associated with Parkinson's disease (PD) in human and neurotoxin-induced animal models. A polyphenol compound, magnolol, was recently proposed as having a potentially restorative effect in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)- or 6-hydroxydopamine-treated animal models. In this study, 18F-FP-(+)-DTBZ PET was used to determine the therapeutic efficacy of magnolol in an MPTP-PD mouse model that was prepared by giving an intraperitoneally (i...
2017: PloS One
https://www.readbyqxmd.com/read/28254232/comparing-the-midterm-outcome-of-single-incision-vaginal-mesh-and-transobturator-vaginal-mesh-in-treating-severe-pelvic-organ-prolapse
#10
Tsai-Hwa Yang, Ling-Ying Wu, Fei-Chi Chuang, Fu-Tsai Kung, Kuan-Hui Huang
OBJECTIVE: The aim of this study is to compare perioperative parameters and midterm clinical outcomes using two different mesh kits: transobturator vaginal mesh (TVM) (both Perigee and Apogee), versus single incision vaginal mesh (SIM) (combined Elevate anterior/apical system and Elevate posterior/apical system) in treating severe pelvic organ prolapse (POP). MATERIALS AND METHODS: This is a retrospective cohort study. During 2008 and 2013, those women with severe POP [POP quantification system (POP-Q), Stage III and Stage IV], who received either TVM or SIM operation, were enrolled for cohort comparison...
February 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28152037/reassessing-the-feasibility-of-the-zygote-score-for-predicting-embryo-viability-in-ivf-icsi-using-the-gnrh-antagonist-protocol-compared-to-the-long-protocol
#11
Pin-Yao Lin, Fu-Jen Huang, Fu-Tsai Kung, Yi-Chi Lin, Hsin-Ju Chiang, Yu-Ju Lin, Kuo-Chung Lan
BACKGROUND: Many factors from the oocyte/sperm or the process of fertilization may affect the zygote formation. The zygote score (Z-score) describes the quality of a human zygote based on its pronuclear morphology, nucleolar precursor bodies, and alignment of polar bodies, and it can be used in the selection process at the zygote stage for embryo transfer or cryopreservation. OBJECTIVE: The aim of this retrospective cohort study was to investigate the relationship between different controlled ovarian stimulation (COS) protocols and the zygote score (Z-score) and to assess the feasibility of the Z-score for predicting embryo survival in the GnRH-antagonist (GnRH-ant) protocol...
2017: PloS One
https://www.readbyqxmd.com/read/28098219/combined-lrrk2-mutation-aging-and-chronic-low-dose-oral-rotenone-as-a-model-of-parkinson-s-disease
#12
Hui-Fang Liu, Philip Wing-Lok Ho, Gideon Chi-Ting Leung, Colin Siu-Chi Lam, Shirley Yin-Yu Pang, Lingfei Li, Michelle Hiu-Wai Kung, David Boyer Ramsden, Shu-Leong Ho
Aging, genetics and environmental toxicity are important etiological factors in Parkinson's disease (PD). However, its pathogenesis remains unclear. A major obstacle is the lack of an appropriate experimental model which incorporates genetic susceptibility, aging and prolonged environmental toxicity. Here, we explored the interplay amongst these factors using mutant LRRK2(R1441G) (leucine-rich-repeat-kinase-2) knockin mice. We found that mutant primary cortical and mesencephalic dopaminergic neurons were more susceptible to rotenone-induced ATP deficiency and cell death...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28077164/erratum-to-novel-sorafenib-analogues-induce-apoptosis-through-shp-1-dependent-stat3-inactivation-in-human-breast-cancer-cells
#13
Chun-Yu Liu, Ling-Ming Tseng, Jung-Chen Su, Kung-Chi Chang, Pei-Yi Chu, Wei-Tien Tai, Chung-Wai Shiau, Kuen-Feng Chen
No abstract text is available yet for this article.
January 11, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/27987771/adhesion-and-metabolic-activity-of-human-corneal-cells-on-pcl-based-nanofiber-matrices
#14
Piotr Stafiej, Florian Küng, Daniel Thieme, Marta Czugala, Friedrich E Kruse, Dirk W Schubert, Thomas A Fuchsluger
In this work, polycaprolactone (PCL) was used as a basic polymer for electrospinning of random and aligned nanofiber matrices. Our aim was to develop a biocompatible substrate for ophthalmological application to improve wound closure in defects of the cornea as replacement for human amniotic membrane. We investigated whether blending the hydrophobic PCL with poly (glycerol sebacate) (PGS) or chitosan (CHI) improves the biocompatibility of the matrices for cell expansion. Human corneal epithelial cells (HCEp) and human corneal keratocytes (HCK) were used for in vitro biocompatibility studies...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27930829/diabetes-hepatocellular-carcinoma-and-mortality-in-hepatitis-c-infected-patients-a-population-based-cohort-study
#15
Ting-Shuo Huang, Chih-Lang Lin, Yu-Chiau Shyu, Chau-Ting Yeh, Kung-Hao Liang, Chi-Chin Sun, Mu-Jie Lu, Rong-Nan Chien
BACKGROUND AND AIM: The effect of diabetes mellitus (DM) on the development of hepatocellular carcinoma (HCC) and all-cause mortality after HCC development in chronic hepatitis C virus (HCV) infected patients remains inconclusive. This cohort study aimed to investigate these issues using the Taiwanese National Health Insurance Research Database. METHODS: We retrieved and enrolled newly diagnosed DM patients with HCV from the Longitudinal Cohort of Diabetes Patients database...
December 8, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27918304/new-fluorous-ponytailed-4-2-2-2-trifluoroethoxy-methyl-pyridinium-halide-salts
#16
Norman Lu, Mani Alagesan, Chi Liang Ho, Rong Jyun Wei, Chih Chieh Kung, Jung Shan Chang, Yuh Sheng Wen, Ling Kang Liu
It is possible that fluorous compounds could be utilized as directing forces in crystal engineering for applications in materials chemistry or catalysis. Although numerous fluorous compounds have been used for various applications, their structures in the solid state remains a lively matter for debate. The reaction of 4-[(2,2,2-trifluoroethoxy)methyl]pyridine with HX (X = I or Cl) yielded new fluorous ponytailed pyridinium halide salts, namely 4-[(2,2,2-trifluoroethoxy)methyl]pyridinium iodide, C8H9F3NO(+)·I(-), (1), and 4-[(2,2,2-trifluoroethoxy)methyl]pyridinium chloride, C8H9F3NO(+)·Cl(-), (2), which were characterized by IR spectroscopy, multinuclei ((1)H, (13)C and (19)F) NMR spectroscopy and single-crystal X-ray diffraction...
December 1, 2016: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/27885991/retraction-mmp-13-is-involved-in-oral-cancer-cell-metastasis
#17
Shun-Hong Huang, Ching-Hsuan Law, Ping-Hsueh Kuo, Ren-Yu Hu, Ching-Chieh Yang, Ting-Wen Chung, Ji-Min Li, Li-Hsun Lin, Yi-Chung Liu, En-Chi Liao, Yi-Ting Tsai, Yu-Shan Wei, Chi-Chen Lin, Chien-Wen Chang, Hsiu-Chuan Chou, Wen-Ching Wang, Margaret Dah-Tsyr Chang, Lu-Hai Wang, Hsing-Jien Kung, Hong-Lin Chan, Ping-Chiang Lyu
HASH(0x46a81c8)
July 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27828511/two-level-modulation-scheme-to-reduce-latency-for-optical-mobile-fronthaul-networks
#18
Jiun-Yu Sung, Chi-Wai Chow, Chien-Hung Yeh, Gee-Kung Chang
A system using optical two-level orthogonal-frequency-division-multiplexing (OFDM) - amplitude-shift-keying (ASK) modulation is proposed and demonstrated to reduce the processing latency for the optical mobile fronthaul networks. At the proposed remote-radio-head (RRH), the high data rate OFDM signal does not need to be processed, but is directly launched into a high speed photodiode (HSPD) and subsequently emitted by an antenna. Only a low bandwidth PD is needed to recover the low data rate ASK control signal...
October 31, 2016: Optics Express
https://www.readbyqxmd.com/read/27751415/validation-of-the-traditional-chinese-version-of-the-prolapse-quality-of-life-questionnaire-p-qol-in-a-mandarin-speaking-taiwanese-population
#19
MULTICENTER STUDY
Fei-Chi Chuang, Li-Ching Chu, Fu-Tsai Kung, Kuan-Hui Huang
OBJECTIVE: To validate the traditional Chinese translated version of the prolapse quality of life questionnaire (P-QOL). MATERIALS AND METHODS: The P-QOL questionnaire was translated into traditional Chinese characters and administered to women recruited from gynecologic outpatient clinics of Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. After the test-retest reliability and internal consistency were established in a pilot study, all participants completed the P-QOL questionnaire and were examined in the lithotomy position using the Pelvic Organ Prolapse Quantification System (POP-Q)...
October 2016: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27742031/distinct-subpopulations-of-hepatitis-c-virus-infectious-cells-with-different-levels-of-intracellular-hepatitis-c-virus-core-protein
#20
Shu-Chi Wang, Jeng-Fu Yang, Chao-Ling Wang, Chung-Feng Huang, Yu-Yin Lin, Yi-You Chen, Chung-Ting Lo, Po-Yen Lee, Kuan-Ta Wu, Chia-I Lin, Meng-Hsuan Hsieh, Hung-Yi Chuang, Chi-Kung Ho, Ming-Lung Yu, Chia-Yen Dai
Chronic infection by hepatitis C virus (HCV) is a major risk factor for the development of hepatocellular carcinoma (HCC). Despite the clear clinical importance of virus-associated HCC, the underlying molecular mechanisms remain largely unclarified. Oxidative stress, in particular, DNA lesions associated with oxidative damage, plays a major role in carcinogenesis, and is strongly linked to the development of many cancers, including HCC. However, in identifying hepatocytes with HCV viral RNA, estimates of the median proportion of HCV-infected hepatocytes have been found as high as 40% in patients with chronic HCV infection...
October 2016: Kaohsiung Journal of Medical Sciences
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