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https://www.readbyqxmd.com/read/29764492/mineral-trioxide-aggregate-affects-cell-viability-and-induces-apoptosis-of-stem-cells-from-human-exfoliated-deciduous-teeth
#1
Chia-Ling Tsai, Mu-Chan Ke, Yi-Hao Chen, Hsi-Kung Kuo, Hun-Ju Yu, Chueh-Tan Chen, Ya-Chi Tseng, Pei-Chin Chuang, Pei-Chang Wu
BACKGROUND: Mineral trioxide aggregate (MTA) is widely used for pulp-capping procedures in permanent teeth and as a gold standard material in endodontics. The aim of the study was to investigate the effect of MTA on cell viability and apoptosis when MTA is directly in contact with Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs). METHODS: MTA was mixed and coated in the bottom of a 24-well plate. SHEDs collected and cultured from normal exfoliated human deciduous teeth (passages 3-4) were seeded on square cover glasses...
May 15, 2018: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29710583/survey-of-selected-personal-care-products-in-surface-water-of-coral-reefs-in-kenting-national-park-taiwan
#2
Te An Kung, Shu Hui Lee, Ting Chi Yang, Wei Hsien Wang
Kenting National Park (KNP) located in the Hengchun Peninsula in southern Taiwan is a popular tourist spot, annually attracting millions of visitors, who engage in water sport and amusement activities. In this region, sewage is directly discharged into the marine environment. In this study, the concentrations of five organic UV filters [benzophenone (BP), 2,4-dihydroxy benzophenone (BP-1), 2-hydroxy-4-methoxy benzophenone (BP-3), 2,2'-dihydroxy-4-methoxy benzophenone (BP-8), and 4-methylbenzylidene camphor], five preservatives [methylparaben (MeP), ethylparaben, propylparaben (PrP), butylparaben, and benzylparaben], one disinfectant [triclosan (TCS)], and twenty-four detergent derivatives [nonylphenol (NP), nonylphenol ethoxylates (NP2EO-NP12EO), octylphenol (OP) and octylphenol ethoxylates OP2EO-OP12EO] were detected in seawater and river water samples collected from eight beaches in KNP and two major river estuaries in the Hengchun Peninsula...
April 23, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29692195/exosomes-and-stem-cells-in-degenerative-disease-diagnosis-and-therapy
#3
Yu-Hsun Chang, Kung-Chi Wu, Horng-Jyh Harn, Shinn-Zong Lin, Dah-Ching Ding
Stroke can cause death and disability, resulting in a huge burden on society. Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by motor dysfunction. Osteoarthritis (OA) is a progressive degenerative joint disease characterized by cartilage destruction and osteophyte formation in the joints. Stem cell therapy may provide a biological treatment alternative to traditional pharmacological therapy. Mesenchymal stem cells (MSCs) are preferred because of their differentiation ability and possible derivation from many adult tissues...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29662779/finite-element-analysis-of-biomechanical-effects-of-total-ankle-arthroplasty-on-the-foot
#4
Yan Wang, Zengyong Li, Duo Wai-Chi Wong, Cheng-Kung Cheng, Ming Zhang
Background: Total ankle arthroplasty is gaining popularity as an alternation to ankle arthrodesis for end-stage ankle arthritis. Owing to the complex anatomical characteristics of the ankle joint, total ankle arthroplasty has higher failure rates. Biomechanical exploration of the effects of total ankle arthroplasty on the foot and ankle is imperative for the precaution of postoperative complications. The objectives of this study are (1) to investigate the biomechanical differences of the foot and ankle between the foot with total ankle arthroplasty and the intact foot and (2) to investigate the performance of the three-component ankle prosthesis...
January 2018: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29662650/hdl-and-associated-factors-stratified-by-sex-and-menopausal-status-results-from-a-community-based-survey-in-taiwan
#5
Huan-Cheng Chang, Chuan-Fa Hsieh, Disline Manli Tantoh, Pei-Chieh Ko, Ya-Yu Kung, Mei-Chi Lin, Yi-Ching Liaw, Yung-Po Liaw
Aim: To investigate factors, especially modifiable factors associated with high-density lipoprotein (HDL) in Taiwanese based on sex and menopausal status. Materials and Methods: Participants comprised 2022 men and 2392 women (1267 menopausal and 1125 non-menopausal) aged ≥30 years who resided in Pingzhen district, Taoyuan from 2006-2011. Their data, obtained through questionnaires and measurements were retrieved from the Li-Shin Hospital. Results: Higher HDL was associated with total cholesterol, underweight, and alcohol drinking in both men and women...
March 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29615745/simple-method-for-de-novo-structural-determination-of-underivatised-glucose-oligosaccharides
#6
Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-Ting Tsai, Chi-Kung Ni
Carbohydrates have various functions in biological systems. However, the structural analysis of carbohydrates remains challenging. Most of the commonly used methods involve derivatization of carbohydrates or can only identify part of the structure. Here, we report a de novo method for completely structural identification of underivatised oligosaccharides. This method, which can provide assignments of linkages, anomeric configurations, and branch locations, entails low-energy collision-induced dissociation (CID) of sodium ion adducts that enable the cleavage of selective chemical bonds, a logical procedure to identify structurally decisive fragment ions for subsequent CID, and the specially prepared disaccharide CID spectrum databases...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29615312/integrated-microrna-and-mrna-expression-profile-analysis-of-tumor-associated-macrophages-after-exposure-to-single-dose-irradiation
#7
Wei-Hsiang Kung, Chi-Lung Lee, Chi-Dung Yang, Ching-Fang Yu, Men-Yee Chiew, Fang-Hsin Chen, Hsien-Da Huang
BACKGROUND: Radiotherapy (RT) is a common approach that accounts for nearly 50% of cancer patient treatment and has the potential for long-term tumor control. Recently, we published a research article on gene expression profiling of tumor-associated macrophages (TAM) that were exposed to ionizing radiation (IR). Single-dose irradiation of tumors could initiate differentially expressed genes in TAM as a time series from irradiated tumors that are associated with the immune response. It is also well known that human cancers are associated with microRNA (miRNA) alterations that are involved in cancer progression...
March 13, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29595662/difference-between-elderly-and-non-elderly-patients-in-using-serum-lactate-level-to-predict-mortality-caused-by-sepsis-in-the-emergency-department
#8
Hsien-Hung Cheng, Fu-Cheng Chen, Meng-Wei Change, Chia-Te Kung, Chi-Yung Cheng, Tsung-Cheng Tsai, Sheng-Yuan Hsiao, Chih-Min Su
Elderly people are more susceptible to sepsis and experience more comorbidities and complications than young adults. Serum lactate is a useful biomarker to predict mortality in patients with sepsis. Lactate production is affected by the severity of sepsis, organ dysfunction, and adrenergic stimulation. Whether the predictive ability of serum lactate will be different between non-elderly and elderly patients is unknown.A retrospective cohort study was conducted to compare the prognostic value of hyperlactatemia in predicting the mortality between elderly (≥65 years) and non-elderly (<65 years) patients with sepsis...
March 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29486713/gallbladder-function-predicts-subsequent-biliary-complications-in-patients-with-common-bile-duct-stones-after-endoscopic-treatment
#9
Tzung-Jiun Tsai, Hoi-Hung Chan, Kwok-Hung Lai, Chih-An Shih, Sung-Shuo Kao, Wei-Chih Sun, E-Ming Wang, Wei-Lun Tsai, Kung-Hung Lin, Hsien-Chung Yu, Wen-Chi Chen, Huay-Min Wang, Feng-Woei Tsay, Huey-Shyan Lin, Jin-Shiung Cheng, Ping-I Hsu
BACKGROUND: In patients with common bile duct stones (CBDS) and intact gallbladder, further management for the gallbladder after the CBDS clearance is still controversial. The relationship between gallbladder motility and the biliary complications were seldom discussed. Our study is to predict the subsequent biliary complications by gallbladder function test using fatty meal sonography (FMS) in patients with CBDS who had been treated by endoscopic retrograde cholangiopancreatography (ERCP)...
February 27, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29471658/excited-state-dissociation-dynamics-of-phenol-studied-by-a-new-time-resolved-technique
#10
Yen-Cheng Lin, Chin Lee, Shih-Huang Lee, Yin-Yu Lee, Yuan T Lee, Chien-Ming Tseng, Chi-Kung Ni
Phenol is an important model molecule for the theoretical and experimental investigation of dissociation in the multistate potential energy surfaces. Recent theoretical calculations [X. Xu et al., J. Am. Chem. Soc. 136, 16378 (2014)] suggest that the phenoxyl radical produced in both the X and A states from the O-H bond fission in phenol can contribute substantially to the slow component of photofragment translational energy distribution. However, current experimental techniques struggle to separate the contributions from different dissociation pathways...
February 21, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29458935/everybody-was-kung-fu-fighting-the-beneficial-effects-of-tai-chi-qigong-and-self-defense-kung-fu-training-on-psychological-and-endocrine-health-in-middle-aged-and-older-men
#11
A Walther, T J Lacker, U Ehlert
BACKGROUND: Higher age is associated to a variety of physical and mental disorders. Age-related changes in steroid secretion have been suggested to be an underlying mechanism leading to frailty, depression, and sexual dysfunction. However, Tai chi qigong and similar forms of exercise have been shown to improve a great variety of health-related parameters in older individuals. METHODS: We examined 56 self-reporting healthy men actively practicing Tai chi qigong and/or self-defense Kung-fu and 55 age-matched self-reporting healthy controls...
February 2018: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/29458911/pharmacogenetic-study-of-pruritus-induced-by-epidural-morphine-for-post-cesarean-section-analgesia
#12
Chia-Chi Kung, Shiou-Sheng Chen, Hong-Jyh Yang, Chih-Jun Lai, Li-Kuei Chen
OBJECTIVE: The mechanism through which neuroaxial morphine causes pruritus has not been elucidated clearly and thoroughly. MATERIALS AND METHODS: a study in 129 female parturients was conducted to investigate the effect of 14 single nucleotide polymorphisms (SNPs) on phenotype (pruritus) induced by neuroaxial (including intrathecal or epidural) morphine for cesarean section. Clinical phenotype, subjective complaints and objective observations were recorded. DNA from blood samples was used to record the SNPs...
February 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29454810/predictors-of-functional-outcome-after-hanging-injury
#13
Chia-Lung Kao, I-Lin Hsu
PURPOSE: Suicide becomes a serious problem in today's society and hanging is a common method of suicide. We want to find the factors which can predict the final functional outcomes of these cases. METHODS: All patients who presented to Accident and Emergency Department (ED) of the National Cheng Kung University Hospital from 1st January 2005 to 31th December 2013 with a hanging injury were included in this study. All cases were divided into good outcome group and bad outcome group according to Glasgow Outcome Scale (GOS)...
April 2018: Chinese Journal of Traumatology, Zhonghua Chuang Shang za Zhi
https://www.readbyqxmd.com/read/29358660/tuning-bandgap-and-surface-wettability-of-nife-2-o-4-driven-by-phase-transition
#14
Sheng-Kai Tong, Po-Wei Chi, Shu-Hsiang Kung, Da-Hua Wei
Stress variation induced bandgap tuning and surface wettability switching of spinel nickel ferrite (NiFe2 O4 , NFO) films were demonstrated and directly driven by phase transition via a post-annealing process. Firstly, the as-deposited NFO films showed hydrophilic surface with water contact angle (CA) value of 80 ± 1°. After post-annealing with designed temperatures ranged from 400 to 700 °C in air ambience for 1 hour, we observed that the crystal structure was clearly improved from amorphous-like/ nanocrystalline to polycrystalline with increasing post-annealing temperature and this phenomenon is attributed to the improved crystallinity combined with relaxation of internal stress...
January 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29240799/inappropriate-self-medication-among-adolescents-and-its-association-with-lower-medication-literacy-and-substance-use
#15
Chun-Hsien Lee, Fong-Ching Chang, Sheng-Der Hsu, Hsueh-Yun Chi, Li-Jung Huang, Ming-Kung Yeh
BACKGROUND: While self-medication is common, inappropriate self-medication has potential risks. This study assesses inappropriate self-medication among adolescents and examines the relationships among medication literacy, substance use, and inappropriate self-medication. METHOD: In 2016, a national representative sample of 6,226 students from 99 primary, middle, and high schools completed an online self-administered questionnaire. Multiple logistic regression analysis was used to examine factors related to inappropriate self-medication...
2017: PloS One
https://www.readbyqxmd.com/read/29235038/simple-approach-for-de-novo-structural-identification-of-mannose-trisaccharides
#16
Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-Ting Tsai, Chi-Kung Ni
Oligosaccharides have diverse functions in biological systems. However, the structural determination of oligosaccharides remains difficult and has created a bottleneck in carbohydrate research. In this study, a new approach for the de novo structural determination of underivatized oligosaccharides is demonstrated. A low-energy collision-induced dissociation (CID) of sodium ion adducts was used to facilitate the cleavage of desired chemical bonds during the dissociation. The selection of fragments for the subsequent CID was guided using a procedure that we built from the understanding of the saccharide dissociation mechanism...
March 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29207018/clinical-significance-of-interleukin%C3%A2-6-and-inducible-nitric-oxide-synthase-in-ketamine%C3%A2-induced-cystitis
#17
Chi-Jung Huang, Fa-Kung Lee, Shao-Kuan Chen, Chih-Cheng Chien, Sheng-Tang Wu, Yen-Chieh Wang
Ketamine is an ionotropic glutamatergic N‑methyl‑D‑aspartate receptor antagonist, which is widely used among recreational drug abusers. Ketamine abusers exhibit substantially reduced bladder capacity, which can lead to urinary frequency. The molecular pathogenesis of ketamine‑induced cystitis has been scarcely reported. Given previous clinical findings, it may be hypothesized that pathological alterations in smooth muscle cells (SMCs) of the urinary bladder serve a crucial role in the mechanism underlying cystitis...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29182404/evaluation-of-the-effect-of-everolimus-on-retinal-pigment-epithelial-cells-and-experimental-proliferative-vitreoretinopathy
#18
Hsi-Kung Kuo, Yi-Hao Chen, Yu-Hsia Kuo, Mu-Chan Ke, Ya-Chi Tseng, Pei-Chang Wu
PURPOSE: Failure of retinal detachment surgery is most commonly due to the development of proliferative vitreoretinopathy (PVR). Everolimus is an inhibitor of mammalian target of rapamycin (mTOR), and is available as oral tablets. In this study, we investigated the effect of everolimus on retinal pigment epithelial cells and modification of the severity of experimental PVR. METHODS: In our in vitro studies, primary culture of retinal pigment epithelium (RPE) cells was obtained from pigmented Rex rabbits...
March 2018: Current Eye Research
https://www.readbyqxmd.com/read/29180361/production-of-1-dodecanol-1-tetradecanol-and-1-12-dodecanediol-through-whole-cell-biotransformation-in-escherichia-coli
#19
Shan-Chi Hsieh, Jung-Hao Wang, Yu-Chen Lai, Ching-Yeuh Su, Kung-Ta Lee
Medium- and long-chain 1-alkanol and α,ω-alkanediols are used in personal care products, in industrial lubricants, and as precursors for polymers synthesized for medical applications. The industrial production of α,ω-alkanediols by alkane hydroxylation primarily occurs at high temperature and pressure using heavy metal catalysts. However, bioproduction has recently emerged as a more economical and environmentally friendly alternative. Among alkane monooxygenases, CYP153A from Marinobacter aquaeolei VT8 (CYP153A M...
February 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29168286/comparative-proteomic-profiling-reveals-a-role-for-cisd2-in-skeletal-muscle-aging
#20
Yi-Long Huang, Zhao-Qing Shen, Chia-Yu Wu, Yuan-Chi Teng, Chen-Chung Liao, Cheng-Heng Kao, Liang-Kung Chen, Chao-Hsiung Lin, Ting-Fen Tsai
Skeletal muscle has emerged as one of the most important tissues involved in regulating systemic metabolism. The gastrocnemius is a powerful skeletal muscle composed of predominantly glycolytic fast-twitch fibers that are preferentially lost among old age. This decrease in gastrocnemius muscle mass is remarkable during aging; however, the underlying molecular mechanism is not fully understood. Strikingly, there is a ~70% decrease in Cisd2 protein, a key regulator of lifespan in mice and the disease gene for Wolfram syndrome 2 in humans, within the gastrocnemius after middle age among mice...
February 2018: Aging Cell
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