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https://www.readbyqxmd.com/read/29137263/melatonin-suppresses-hepatocellular-carcinoma-progression-via-lncrna-cps1-it-mediated-hif-1%C3%AE-inactivation
#1
Tong-Hong Wang, Chi-Hao Wu, Chau-Ting Yeh, Shih-Chi Su, Shih-Min Hsia, Kung-Hao Liang, Chin-Chuan Chen, Chuen Hsueh, Chi-Yuan Chen
Melatonin is the primary pineal hormone that relays light/dark cycle information to the circadian system. It was recently reported to exert intrinsic antitumor activity in various cancers. However, the regulatory mechanisms underlying the antitumor activity of melatonin are poorly understood. Moreover, a limited number of studies have addressed the role of melatonin in hepatocellular carcinoma (HCC), a major life-threatening malignancy in both sexes in Taiwan. In this study, we investigated the antitumor effects of melatonin in HCC and explored the regulatory mechanisms underlying these effects...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29114176/detecting-triple-vessel-disease-with-cadmium-zinc-telluride-based-single-photon-emission-computed-tomography-using-the-intensity-signal-to-noise-ratio-between-rest-and-stress-studies
#2
Yu-Hua Dean Fang, Tzu-Pei Su, Chi-Jen Chang, Kung-Chu Ho, May Su, Tzu-Chen Yen
The purpose of this study was to investigate if a novel parameter, the stress-to-rest ratio of the signal-to-noise ratio (RSNR) obtained with a cadmium zinc telluride (CZT) SPECT scanner, could be used to distinguish triple-vessel disease (TVD) patients. Methods. One hundred and two patients with suspected coronary artery disease were retrospectively involved. Each subject underwent a Tl-201 SPECT scan and subsequent coronary angiography. Subjects were separated into TVD (n = 41) and control (n = 61) groups based on coronary angiography results using 50% as the stenosis cutoff...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29104465/effect-of-coriolus-versicolor-mycelia-extract-on-exercise-performance-and-physical-fatigue-in-mice
#3
Chun-Sheng Ho, Yu-Tang Tung, Woon-Man Kung, Wen-Ching Huang, Wing-Ki Leung, Chi-Chang Huang, Jyh-Horng Wu
In this study, Coriolus versicolor mycelia (CVM) was evaluated the ergogenic and anti-fatigue activities. Male ICR mice were divided into four groups (n = 8/group) to receive vehicle or CVM by oral gavage for 4 weeks at 0, 615, 1230 or 3075 mg/kg/day, which were respectively designated the vehicle, CVM-1X, CVM-2X and CVM-5X groups. Forelimb grip strength, endurance swimming time, and levels of physical fatigue-associated parameters serum lactate, ammonia, glucose and creatine kinase (CK) after physical challenge were performed to evaluate exercise performance and anti-fatigue activity...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29100283/circulating-microparticles-are-prognostic-biomarkers-in-advanced-non-small-cell-lung-cancer-patients
#4
Chin-Chou Wang, Chia-Cheng Tseng, Huang-Chih Chang, Kuo-Tung Huang, Wen-Feng Fang, Yu-Mu Chen, Cheng-Ta Yang, Chang-Chun Hsiao, Meng-Chih Lin, Chi-Kung Ho, Hon-Kan Yip
We investigated whether circulating microparticles (MPs) could serve as prognostic biomarkers in non-small cell lung cancer (NSCLC) patients. We enrolled 25 control subjects and 136 NSCLC patients categorized into disease-progression (DP, n=42) and disease-control (DC, n=94) groups. Flow cytometric analysis showed that levels of four types of circulating microparticles (EDAc-MPs, EDAp-MPs, PDAc-MPs and PDAp-MPs) were higher in the study patients than the control subjects (P < 0.04). DP patients showed poor initially performance status and more non-adenocarcinomas than DC patients...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29098808/casticin-impairs-cell-growth-and-induces-cell-apoptosis-via-cell-cycle-arrest-in-human-oral-cancer-scc-4-cells
#5
Guan-Ling Chou, Shu-Fen Peng, Ching-Lung Liao, Heng-Chien Ho, Kung-Wen Lu, Jin-Cherng Lien, Ming-Jen Fan, Kuang-Chi La, Jing-Gung Chung
Casticin, a polymethoxyflavone, present in natural plants, has been shown to have biological activities including anti-cancer activities. Herein, we investigated the anti-oral cancer activity of casticin on SCC-4 cells in vitro. Viable cells, cell cycle distribution, apoptotic cell death, reactive oxygen species (ROS) production, and Ca(2+) production, levels of ΔΨm and caspase activity were measured by flow cytometric assay. Cell apoptosis associated protein expressions were examined by Western blotting and confocal laser microscopy...
November 3, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29028684/toxic-metals-increase-serum-tumor-necrosis-factor-%C3%AE-levels-modified-by-essential-elements-and-different-types-of-tumor-necrosis-factor-%C3%AE-promoter-single-nucleotide-polymorphisms
#6
Yung-Cheng Huang, Wei-Chiao Chang, Ya-Han Shan, Chao-Yi Lin, Chao-Ling Wang, Chia-Yen Dai, Chi-Kung Ho, Ming-Tsang Wu, Hung-Yi Chuang
BACKGROUND: Lead (Pb), cadmium (Cd), and arsenic (As) could cause health issues through oxidative stress that is indicated in the elevated tumor necrosis factor-alpha (TNF-α). However, some of the essential elements-selenium (Se), zinc (Zn), cobalt (Co), and copper (Cu)-are cofactors or structural components of antioxidant enzymes. It is suggested that single-nucleotide polymorphisms (SNPs) in the TNF-α gene have different TNF-α responses. This study aims to evaluate the effect of serum TNF-α levels through the interactions between toxic metals and essential elements and how the interactions between the toxic metals and TNF-α SNPs (-1031 T > C, -863 C > A, -857 C > T, -308 G > A, -238 G > A) influence serum TNF-α levels...
October 2017: Epidemiology
https://www.readbyqxmd.com/read/28993442/in-room-assessment-of-intravascular-velocity-from-time-resolved-rotational-angiography-in-patients-with-arteriovenous-malformation-a-pilot-study
#7
Chung Jung Lin, Huai Che Yang, Ai Chi Chien, Wan Yuo Guo, Chih Chun Wu, Sheng Che Hung, Ko Kung Chen, Hsiu Mei Wu, Chao Bao Luo, Wei Fa Chu, Jia Sheng Hong, Chun Shien Frank Wu
BACKGROUND: Time-resolved rotational angiography (t-RA) enables interventionists to better comprehend complex arteriovenous malformations (AVMs), thereby facilitating endovascular treatment. However, its use in evaluating hemodynamic changes has rarely been explored. OBJECTIVE: This study uses t-RA to estimate intravascular flow in patients with AVM to compare this with flow in the normal population. METHODS: Patients with available t-RA scans were prospectively categorized into one of three groups: hemorrhagic AVM, non-hemorrhagic AVM and control...
October 9, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28988665/the-epidemiology-of-non-typhoidal-salmonella-gastroenteritis-and-campylobacter-gastroenteritis-in-pediatric-inpatients-in-northern-taiwan
#8
Chien-Fang Tseng, Nan-Chang Chiu, Ching-Ying Huang, Daniel Tsung-Ning Huang, Lung Chang, Yen-Hsin Kung, Fu-Yuan Huang, Hsin Chi
BACKGROUND: Campylobacter and Non-typhoidal Salmonella (NTS) are the two most common bacterial pathogens associated with acute gastroenteritis in children. This study aims to elucidate the epidemiology of Campylobacter and NTS gastroenteritis and develop a scoring system to differentiate them. MATERIALS AND METHODS: This retrospective study enrolled 886 children ≤18 years of age, hospitalized due to acute gastroenteritis with stool culture-proven Campylobacter or NTS infection from July 2012 to December 2015...
September 27, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28988664/epidemiology-and-antimicrobial-susceptibility-of-non-typeable-haemophilus-influenzae-in-otitis-media-in-taiwanese-children
#9
Ying-Chun Cho, Nan-Chang Chiu, Fu-Yuan Huang, Daniel Tsung-Ning Huang, Lung Chang, Ching-Ying Huang, Yen-Hsin Kung, Kuo-Sheng Lee, Hsin Chi
BACKGROUND: Concerns about non-typeable Haemophilus influenzae (NTHi) in otitis media (OM) have grown after the introduction of pneumococcal conjugate vaccine (PCV). We aim to better understand the clinical role of NTHi in pediatric OM. METHODS: Middle ear fluid samples from children <18 years with OM were obtained from 2010 to 2015. For culture-positive episodes (Streptococcus pneumoniae, H. influenzae, Moraxella catarrhalis, and Streptococcus pyogenes), patients' demographic and clinical information were reviewed and analyzed...
September 18, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28985720/effects-of-homocysteine-on-white-matter-diffusion-parameters-in-alzheimer-s-disease
#10
Chen-Chang Lee, Shih-Wei Hsu, Chi-Wei Huang, Wen-Neng Chang, Sz-Fan Chen, Ming-Kung Wu, Chiung-Chih Chang, Lain-Chyr Hwang, Po-Chou Chen
BACKGROUND: The clinical features of Alzheimer's disease (AD) are related to brain network degeneration, and hyperhomocysteinemia is related to greater white matter hyperintensities. We investigated the changes in four diffusion tensor imaging parameters in the white matter of patients with early stage AD, examined their associations with homocysteine level, and tested the clinical significance of the diffusion tensor imaging parameters and homocysteine level in correlation analysis with cognitive test scores...
October 6, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28983679/safety-of-omitting-routine-bleeding-tests-prior-to-image-guided-musculoskeletal-core-needle-biopsy
#11
Yuri Shif, Justin W Kung, Colm J McMahon, Jennifer Ni Mhuircheartaigh, Yu Ching Lin, Megan E Anderson, Jim S Wu
PURPOSE: To evaluate the safety of withholding preprocedure international normalized ratio (INR) and platelet testing in patients undergoing musculoskeletal (MSK) core needle biopsy (CNB). MATERIAL AND METHODS: Initially, a retrospective review of 1,162 consecutive patients undergoing MSK CNB with preprocedural INR and platelet testing was performed. Clinical (age, gender, bleeding disorder, liver disease, anticoagulation use, INR > 2, platelet count <50,000/ul) and biopsy factors (imaging modality, lesion type, biopsy needle gauge, number biopsy samples) were tested for association with bleeding complications...
October 6, 2017: Skeletal Radiology
https://www.readbyqxmd.com/read/28974646/factors-associated-with-re-intubation-within-14-days-after-ventilator-liberation
#12
Chia-Chen Chu, Chin-Jung Liu, Suh-May Yen, Wen-Yu Chou, Pei-Tseng Kung, Yuh-Show Tsai, Wen-Chen Tsai
BACKGROUND: According to Taiwan's integrated delivery system policy, ventilator-dependent patients are successfully liberated from mechanical ventilation in accordance with step-down care. However, premature discharge affects the 14-d readmission quality index. Therefore, we explored the risk and related factors of subjects liberated from mechanical ventilation who were re-intubated within 14 d. METHODS: This retrospective study analyzed a cohort of ventilator-dependent subjects 17 y of age and older using a population-based database from the Taiwan National Health Research Institutes Database from 2006 to 2010...
October 3, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28950692/effect-of-carvacrol-on-ca%C3%A2-%C3%A2-%C2%BA-movement-and-viability-in-pc3-human-prostate-cancer-cells
#13
Chi-Ting Horng, Chiang-Ting Chou, Te-Kung Sun, Wei-Zhe Liang, Chun-Chi Kuo, Jue-Long Wang, Pochuen Shieh, Chung-Ren Jan
Carvacrol, a monoterpenic phenol compound, has been shown to possess various biological effects in different models. However, the effect of carvacrol on intracellular Ca²⁺ and its related physiology in human prostate cancer is unknown. This study explored the effect of carvacrol on cytosolic free Ca²⁺ levels ([Ca²⁺]i) and viability in PC3 human prostate cancer cells. Fura-2, a Ca²⁺- sensitive fluorescent dye, was used to assess [Ca²⁺]i. Cell viability was measured by the detecting reagent WST-1...
October 31, 2017: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/28943959/cyclin-d1-overexpression-correlates-with-poor-tumor-differentiation-and-prognosis-in-gastric-cancer
#14
Yan-Shen Shan, Hui-Ping Hsu, Ming-Derg Lai, Yu-Hsuan Hung, Chih-Yang Wang, Meng-Chi Yen, Yi-Ling Chen
Overexpression of cyclin D is associated with the molecular tumorigenesis of gastric cancer. The purpose of the present study was to investigate the expression of cyclin D in human gastric cancer and to determine the potential correlations between cyclin D expression and clinicopathological characteristics of specific histological types, as well as its prognostic significance. In the present study, the expression of the cyclin D1 (CCND1), cyclin D2 (CCND2) and cyclin D3 (CCND3) genes in gastric cancer patients was explored using the Oncomine database, and their correlation with overall survival (OS) and progression-free survival (PFS) was evaluated using Kaplan-Meier analysis...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28918242/predictive-value-of-thomsen-friedenreich-antigen-activation-for-streptococcus-pneumoniae-infection-and-severity-in-pediatric-lobar-pneumonia
#15
Chia-Jung Chang, Nan-Chang Chiu, Fu-Yuan Huang, Daniel Tsung-Ning Huang, Lung Chang, Ching-Ying Huang, Yen-Hsin Kung, Hsin Chi
BACKGROUND: Most cases of complicated pneumonia in children are caused by pneumococcal infections. Thomsen-Friedenreich antigen (TA) is present on erythrocytes, platelets and glomeruli, and it can be activated during pneumococcal infection. The aim of this study was to investigate the predictive value of TA activation for pneumococcal infection and association with the severity of complicated pneumonia. MATERIALS AND METHODS: Patients with lobar pneumonia were routinely tested for TA at the Department of Pediatrics, Mackay Memorial Hospital from January 2010 to December 2015...
September 2, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28900621/using-a-cloud-computing-system-to-reduce-door-to-balloon-time-in-acute-st-elevation-myocardial-infarction-patients-transferred-for-percutaneous-coronary-intervention
#16
Chi-Kung Ho, Fu-Cheng Chen, Yung-Lung Chen, Hui-Ting Wang, Chien-Ho Lee, Wen-Jung Chung, Cheng-Jui Lin, Shu-Kai Hsueh, Shin-Chiang Hung, Kuan-Han Wu, Chu-Feng Liu, Chia-Te Kung, Cheng-I Cheng
BACKGROUND: This study evaluated the impact on clinical outcomes using a cloud computing system to reduce percutaneous coronary intervention hospital door-to-balloon (DTB) time for ST segment elevation myocardial infarction (STEMI). METHODS: A total of 369 patients before and after implementation of the transfer protocol were enrolled. Of these patients, 262 were transferred through protocol while the other 107 patients were transferred through the traditional referral process...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28882583/clinical-and-epidemiological-characteristics-in-hospitalized-young-children-with-acute-gastroenteritis-in-southern-taiwan-according-to-major-pathogens
#17
Ning Chung, Shih-Min Wang, Ching-Fen Shen, Fu-Chun Kuo, Tzong-Shiann Ho, Chao Agnes Hsiung, Jung-Jung Mu, Fang-Tzy Wu, Li-Min Huang, Yi-Chuan Huang, Yhu-Chering Huang, Hsin Chi, Hsiao-Chuan Lin, Ching-Chuan Liu
BACKGROUND: Acute gastroenteritis (AGE) can be caused by a wide array of pathogens, including bacteria, viruses, and parasites. A prospective study to investigate the epidemiology and clinical presentation in young children hospitalized with AGE was conducted in a medical center in southern Taiwan. METHODS: Patients aged less than 5 years who was hospitalized due to AGE in National Cheng Kung University Hospital were enrolled from July 2014 to June 2016. The demographic information, clinical features and laboratory data were collected by chart reviews, and stool samples were sent to Centers of Disease Control, Taiwan (Taiwan CDC) for a panel of pathogen identification consisting of two viruses, nine bacteria, and five parasites...
August 31, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28870784/isotypes-of-autoantibodies-against-differentially-expressed-novel-malondialdehyde-modified-peptide-adducts-in-serum-of-taiwanese-women-with-rheumatoid-arthritis
#18
Chen-Chung Liao, Yu-Sheng Chang, Chao-Wen Cheng, Wei-Ming Chi, Kai-Leun Tsai, Wei-Jung Chen, Ting-Shuan Kung, Chih-Chun Tai, Yi-Fang Lin, Hung-Tse Lin, Yi-Ying Lu, Ching-Yu Lin
This study identified and validated four differentially expressed novel malondialdehyde (MDA)-modified peptide adducts and evaluated autoantibodies against native and MDA-modified peptides among Taiwanese women patients with rheumatoid arthritis (RA), osteoarthritis (OA) and healthy controls (HCs). Ig kappa chain C region(76-99), alpha-1-antitrypsin(284-298), alpha-2-macroglobulin(824-841), and apolipoprotein B-100(4022-4040) exhibiting 2-fold differences in relative modification ratios were identified by concanavalin A (Con A) affinity chromatography, 1D SDS-PAGE, in-gel digestion, nano-LC/MS/MS and nano-LC/MS using pooled serum-derived Con A-captured proteins from 9 RA and 9 age-matched HCs...
January 6, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/28827659/tdag8-trpv1-and-asic3-involved-in-establishing-hyperalgesic-priming-in-experimental-rheumatoid-arthritis
#19
Wei-Shan Hsieh, Chia-Chi Kung, Shir-Ly Huang, Shih-Chang Lin, Wei-Hsin Sun
Rheumatoid arthritis (RA), characterized by chronic inflammation of synovial joints, is often associated with ongoing pain and increased pain sensitivity. High hydrogen ion concentration (acidosis) found in synovial fluid in RA patients is associated with disease severity. Acidosis signaling acting on proton-sensing receptors may contribute to inflammation and pain. Previous studies focused on the early phase of arthritis (<5 weeks) and used different arthritis models, so elucidating the roles of different proton-sensing receptors in the chronic phase of arthritis is difficult...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815763/does-low-energy-collision-induced-dissociation-of-lithiated-and-sodiated-carbohydrates-always-occur-at-anomeric-carbon-of-the-reducing-end
#20
Shang-Ting Tsai, Jien-Lian Chen, Chi-Kung Ni
RATIONALE: Collision-induced dissociation (CID) tandem mass spectrometry is one of the major methods in the structural determination of carbohydrates. Previous experimental studies and theoretical investigation of lithiated and sodiated underivatized carbohydrates seem to indicate that dehydration reactions and cross-ring dissociation of low-energy CID mainly occur at the anomeric carbon of the reducing end. However, these studies only investigated a few carbohydrates. METHODS: ESI-MS/MS spectra of [M + Li](+) and [M + Na](+) ions of several (18) O1-labeled monosaccharides and disaccharides at O1 of the reducing end were studied using a linear ion trap mass spectrometer...
November 15, 2017: Rapid Communications in Mass Spectrometry: RCM
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