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Masaru Ide, Shinichi Koba, Naoko Sueoka-Aragane, Akemi Sato, Yuri Nagano, Takuya Inoue, Noriyuki Misago, Yutaka Narisawa, Shinya Kimura, Eisaburo Sueoka
: BRAF gene mutations have been observed in 30-50 % of malignant melanoma patients. Recent development of therapeutic intervention using BRAF inhibitors requires an accurate and rapid detection system for BRAF mutations. In addition, the clinical characteristics of the melanoma associated with BRAF mutations in Japanese patients have not been investigated on a large scale evaluation. We recently established quenching probe system (QP) for detection of an activating BRAF mutation, V600E and evaluated 113 melanoma samples diagnosed in Saga University Hospital from 1982 to 2011...
October 21, 2016: Pathology Oncology Research: POR
Claudia Canzonetta, Manuela Leo, Salvatore Rocco Guarino, Arianna Montanari, Silvia Francisci, Patrizia Filetici
In budding yeast, growth through fermentation and/or respiration is dependent on the type of carbon source present in the medium. SAGA complex is the main acetylation complex and is required, together with Rtg factors, for nucleus-mitochondria communication and transcriptional activation of specific nuclear genes. Even though acetylation is necessary for mitochondria activity and respiratory pathways the direct role of histone acetyltransferases and SAGA complex has never been investigated directly. In this study we demonstrate, for the first time, that Gcn5 and SAGA are needed for respiratory metabolism and oxygen consumption...
October 11, 2016: Biochimica et Biophysica Acta
Saga Elise Mariansdatter, Andreas Halgreen Eiset, Kirstine Kobberøe Søgaard, Christian Fynbo Christiansen
BACKGROUND: Sepsis and severe sepsis are common conditions in hospital settings, and are associated with high rates of morbidity and mortality, but reported incidences vary considerably. In this literature review, we describe the variation in reported population-based incidences of sepsis and severe sepsis. We also examine methodological and demographic differences between studies that may explain this variation. METHODS: We carried out a literature review searching three major databases and reference lists of relevant articles, to identify all original studies reporting the incidence of sepsis or severe sepsis in the general population...
October 12, 2016: BMC Medical Research Methodology
Kavita J Rangan, Virginia A Pedicord, Yen-Chih Wang, Byungchul Kim, Yun Lu, Shai Shaham, Daniel Mucida, Howard C Hang
The intestinal microbiome modulates host susceptibility to enteric pathogens, but the specific protective factors and mechanisms of individual bacterial species are not fully characterized. We show that secreted antigen A (SagA) from Enterococcus faecium is sufficient to protect Caenorhabditis elegans against Salmonella pathogenesis by promoting pathogen tolerance. The NlpC/p60 peptidoglycan hydrolase activity of SagA is required and generates muramyl-peptide fragments that are sufficient to protect C. elegans against Salmonella pathogenesis in a tol-1-dependent manner...
September 23, 2016: Science
Yosuke Saga, Yoshihisa Nakayama, Ken-Ichi Inoue, Tomoko Yamagata, Masashi Hashimoto, Léon Tremblay, Masahiko Takada, Eiji Hoshi
The thalamic reticular nucleus (TRN) collects inputs from the cerebral cortex and thalamus and, in turn, sends inhibitory outputs to the thalamic relay nuclei. This unique connectivity suggests that the TRN plays a pivotal role in regulating information flow through the thalamus. Here, we analyzed the roles of TRN neurons in visually guided reaching movements. We first used retrograde transneuronal labeling with rabies virus, and showed that the rostro-dorsal sector of the TRN (TRNrd) projected disynaptically to the ventral premotor cortex (PMv)...
October 5, 2016: European Journal of Neuroscience
Koichi Watanabe, Tsuyoshi Saga, Joe Iwanaga, Yoko Tabira, Koh-Ichi Yamaki
INTRODUCTION: The transversus nuchae muscle appears inconsistently in the occipital region. It has gained attention as one of the muscles composing the superficial musculoaponeurotic system (SMAS). The purpose of this study was to clarify its detailed anatomical features. MATERIALS AND METHODS: We examined 124 sides of 62 cadavers. The transversus nuchae muscle was identified when present and examined after it had been completely exposed. We also examined its relationship to the occipital cutaneous nerves...
September 29, 2016: Clinical Anatomy
Kunio Shimada, Norihiko Saga
Sensors are essential to the fulfillment of every condition of haptic technology, and they need simultaneously to sense shear stress as well as normal force, and temperature. They also must have a strong and simple structure, softness, and large extension. To achieve these conditions simultaneously, we enhanced the sensitivity of sensors utilizing natural rubber (NR)-latex through the application of electrolytic polymerization focused on the isoprene C=C bonds in natural rubbers such as NR-latex, and then applied a magnetic field and magnetic compound fluid (MCF) as magnetically responsive fluid...
2016: Sensors
Kazuma Kamata, Kaori Shinmyozu, Jun-Ichi Nakayama, Masanori Hatashita, Hiroyuki Uchida, Masaya Oki
In eukaryotic cells, there are two chromatin states, silenced and active, and the formation of a so-called boundary plays a critical role in demarcating these regions; however, the mechanisms underlying boundary formation are not well understood. In this study, we focused on S. cerevisiae ADA1, a gene previously shown to encode a protein with a robust boundary function. Ada1 is a component of the histone modification complex Spt-Ada-Gcn5-acetyltransferase (SAGA) and the SAGA-like (SLIK) complex, and it helps to maintain the integrity of these complexes...
October 2016: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Taro Yamashita, Mitsuharu Ueda, Nobuyuki Saga, Kiyonori Nanto, Masayoshi Tasaki, Teruaki Masuda, Yohei Misumi, Seitaro Oda, Akiko Fujimoto, Tomoko Amano, Kotaro Takamatsu, Satoshi Yamashita, Konen Obayashi, Hirotaka Matsui, Yukio Ando
No abstract text is available yet for this article.
September 20, 2016: Amyloid: the International Journal of Experimental and Clinical Investigation
Sarah M Rösler, Katharina Kramer, Iris Finkemeier, Hans-Ulrich Humpf, Bettina Tudzynski
Post-translational modification of histones is a crucial mode of transcriptional regulation in eukaryotes. A well-described acetylation modifier of certain lysine residues is the Spt-Ada-Gcn5 acetyltransferase (SAGA) complex assembled around the histone acetyltransferase Gcn5 in Saccharomyces cerevisiae. We identified and characterized the SAGA complex in the rice pathogen Fusarium fujikuroi, well-known for producing a large variety of secondary metabolites (SMs). By using a co-immunoprecipitation approach, almost all of the S...
September 19, 2016: Molecular Microbiology
Sakib Motalib, David J Dries
No abstract text is available yet for this article.
September 2016: Air Medical Journal
Masanobu Miura, Yasuhiko Sakata, Satoshi Miyata, Nobuyuki Shiba, Jun Takahashi, Kotaro Nochioka, Tsuyoshi Takada, Chiharu Saga, Tsuyoshi Shinozaki, Masafumi Sugi, Makoto Nakagawa, Nobuyo Sekiguchi, Tatsuya Komaru, Atsushi Kato, Mitsumasa Fukuchi, Eiji Nozaki, Tetsuya Hiramoto, Kanichi Inoue, Toshikazu Goto, Masatoshi Ohe, Kenji Tamaki, Setsuro Ibayashi, Nobumasa Ishide, Yukio Maruyama, Ichiro Tsuji, Hiroaki Shimokawa
BACKGROUND: There is no robust evidence of pharmacological interventions to improve mortality in heart failure (HF) patients with preserved left ventricular ejection fraction (LVEF) (HFpEF). In this subanalysis study of the SUPPORT Trial, we addressed the influence of LVEF on the effects of olmesartan in HF. METHODS AND RESULTS: Among 1,147 patients enrolled in the SUPPORT Trial, we examined 429 patients with reduced LVEF (HFrEF, LVEF <50%) and 709 with HFpEF (LVEF ≥50%)...
September 23, 2016: Circulation Journal: Official Journal of the Japanese Circulation Society
Quan Wu, Kurumi Fukuda, Yuzuru Kato, Zhi Zhou, Chu-Xia Deng, Yumiko Saga
The differential programming of sperm and eggs in gonads is a fundamental topic in reproductive biology. Although the sexual fate of germ cells is believed to be determined by signaling factors from sexually differentiated somatic cells in fetal gonads, the molecular mechanism that determines germ cell fate is poorly understood. Herein, we show that mothers against decapentaplegic homolog 4 (SMAD4) in germ cells is required for female-type differentiation. Germ cells in Smad4-deficient ovaries respond to retinoic acid signaling but fail to undergo meiotic prophase I, which coincides with the weaker expression of genes required for follicular formation, indicating that SMAD4 signaling is essential for oocyte differentiation and meiotic progression...
September 2016: PLoS Biology
Toshiki Endo, Jia Wenting, Atsuhiro Nakagawa, Hidenori Endo, Yuto Sagae, Masaki Iwasaki, Teiji Tominaga
Background Surgery for intramedullary tumors is technically demanding because it requires surgical resection along with functional preservation of the spinal cord. The water jet dissector is an emerging tool in neurologic surgeries and a novel tool in spinal cord surgeries. This article evaluates the usefulness and safety of water jet dissection in an experimental study. Methods A pulsed water jet was applied to dissect the posterior median sulcus of the spinal cords of seven anesthetized pigs. In four pigs, the water jet was delivered on the dorsal spinal cord at different input voltages (5, 10, and 15 V) and for durations of either 15 or 30 seconds...
September 7, 2016: Journal of Neurological Surgery. Part A, Central European Neurosurgery
Laura D Gallego, Medini Ghodgaonkar Steger, Anton A Polyansky, Tobias Schubert, Bojan Zagrovic, Ning Zheng, Tim Clausen, Franz Herzog, Alwin Köhler
Cotranscriptional ubiquitination of histone H2B is key to gene regulation. The yeast E3 ubiquitin ligase Bre1 (human RNF20/40) pairs with the E2 ubiquitin conjugating enzyme Rad6 to monoubiquitinate H2B at Lys123. How this single lysine residue on the nucleosome core particle (NCP) is targeted by the Rad6-Bre1 machinery is unknown. Using chemical cross-linking and mass spectrometry, we identified the functional interfaces of Rad6, Bre1, and NCPs in a defined in vitro system. The Bre1 RING domain cross-links exclusively with distinct regions of histone H2B and H2A, indicating a spatial alignment of Bre1 with the NCP acidic patch...
September 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
Wenqian Li, Boyko S Atanassov, Xianjiang Lan, Ryan D Mohan, Selene K Swanson, Aimee T Farria, Laurence Florens, Michael P Washburn, Jerry L Workman, Sharon Y R Dent
The SAGA complex contains two enzymatic modules, which house histone acetyltransferase (HAT) and deubiquitinase (DUB) activity. USP22 is the catalytic subunit of the DUB module, but two adaptor proteins, ATXN7L3 and ENY2, are necessary for DUB activity towards histone H2Bub1 and other substrates. ATXN7L3B shares 74% identity with the N-terminal region of ATXN7L3, but the functions of ATXN7L3B are not known. Here we report that ATXN7L3B interacts with ENY2, but not other SAGA components. Even though ATXN7L3B localizes in the cytoplasm, ATXN7L3B overexpression increases H2Bub1 levels, while overexpression of ATXN7L3 decreases H2Bub1 levels...
September 6, 2016: Molecular and Cellular Biology
Dharmaprakash Viszwapriya, Ganapathy Ashwinkumar Subramenium, Udayakumar Prithika, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian
The present study demonstrates the antivirulence potential of betulin, an abundantly available triterpenoid against Streptococcus pyogenes, a multi-virulent and exclusive human pathogen. Crystal violet assay and microscopic examination revealed that betulin (100 μg mL(-1)) exhibits surface independent antibiofilm activity and mitigates extracellular polymeric substance production. Betulin treatment enhanced the rate of auto-aggregation in liquid medium. Results of real-time PCR and biochemical assays demonstrated that, betulin suppresses the expression of ropB core regulon, sagA and dltA, which correspondingly affects SpeB production, hemolysis and cell surface hydrophobicity, for the observed impairment in virulence and biofilm formation...
September 4, 2016: Pathogens and Disease
Yongjiu Feng, Yan Liu
The world's coastal regions are experiencing rapid urbanization coupled with increased risk of ecological damage and storm surge related to global climate and sea level rising. This urban development issue is particularly important in China, where many emerging coastal cities are being developed. Lingang New City, southeast of Shanghai, is an excellent example of a coastal city that is increasingly vulnerable to environmental change. Sustainable urban development requires planning that classifies and allocates coastal lands using objective procedures that incorporate changing environmental conditions...
September 2016: Environmental Monitoring and Assessment
Antonio González-Pérez, María E Sáez, Saga Johansson, Anders Himmelmann, Luis A García Rodríguez
BACKGROUND: Bleeding events have been associated with the use of antiplatelet agents. This study estimated the incidence of bleeding events in patients previously hospitalized for a serious coronary event and determined the risks of bleeding associated with the use of acetylsalicylic acid (ASA) and/or clopidogrel. METHODS: A UK primary care database was used to identify 27,707 patients aged 50 to 84 years, hospitalized for a serious coronary event during 2000 to 2007 and who were alive 30 days later (start date)...
2016: BMC Cardiovascular Disorders
Angie Rantell, Linda Cardozo, Vik Khullar
INTRODUCTION: In clinical practice and in research patient-centred outcomes are often utilised to help improve communication between patients and clinicians and to help manage expectations from treatment. However, many of these goals are generic and do not adequately capture the details of day to day life that bother patients the most and that they hope will improve with therapy. This study aimed to understand what are the goals of patients with overactive bladder symptoms in the UK who were taking part in a clinical trial and to assess goal achievement...
August 26, 2016: Neurourology and Urodynamics
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