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https://www.readbyqxmd.com/read/29353404/dna-methylation-rather-than-single-nucleotide-polymorphisms-regulates-the-production-of-an-aberrant-splice-variant-of-il6r-in-mastitic-cows
#1
Yan Zhang, Xiuge Wang, Qiang Jiang, Haisheng Hao, Zhihua Ju, Chunhong Yang, Yan Sun, Changfa Wang, Jifeng Zhong, Jinming Huang, Huabin Zhu
Interleukin-6 receptor-alpha (IL6R) interacts with IL6 and forms a ligand-receptor complex, which can stimulate various cellular responses, such as cell proliferation, cell differentiation, and activation of inflammatory processes. Both genetic mutation and epigenetic modification regulate gene transcription. We identified a novel splice variant of bovine IL6R, designated as IL6R-TV, which is characterized by the skipping of exon 2 of the NCBI-referenced IL6R gene (IL6R-reference). The expression levels of IL6R-TV and IL6R-reference transcripts were lower in normal mammary gland tissues...
January 20, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29353403/genetic-polymorphism-in-hsp90aa1-gene-is-associated-with-the-thermotolerance-in-chinese-holstein-cows
#2
T M Badri, K L Chen, M A Alsiddig, Lian Li, Yafei Cai, G L Wang
The heat shock protein 90 (Hsp90) is a copious and ubiquitous molecular chaperone which plays an essential role in many cellular biological processes. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the Hsp90AA1 gene and to determine their association with heat stress traits in Chinese Holstein cattle breed. Direct sequencing was used to identify new SNPs. Luciferase reporter assay methods were used to assess g.- 87G > C and g.4172A > G loci in the promoter activity and 3'-UTR, respectively...
January 20, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29353386/population-based-dose-response-analysis-of-liver-transcriptional-response-to-trichloroethylene-in-mouse
#3
Abhishek Venkatratnam, John S House, Kranti Konganti, Connor McKenney, David W Threadgill, Weihsueh A Chiu, David L Aylor, Fred A Wright, Ivan Rusyn
Studies of gene expression are common in toxicology and provide important clues to mechanistic understanding of adverse effects of chemicals. Most prior studies have been performed in a single strain or cell line; however, gene expression is heavily influenced by the genetic background, and these genotype-expression differences may be key drivers of inter-individual variation in response to chemical toxicity. In this study, we hypothesized that the genetically diverse Collaborative Cross mouse population can be used to gain insight and suggest mechanistic hypotheses for the dose- and genetic background-dependent effects of chemical exposure...
January 20, 2018: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/29353353/peripheral-th17-treg-imbalance-in-elderly-patients-with-ischemic-stroke
#4
Sanam Dolati, Majid Ahmadi, Mohammad Khalili, Ali Akabar Taheraghdam, Homayoon Siahmansouri, Zohreh Babaloo, Leili Aghebati-Maleki, Farhad Jadidi-Niaragh, Vahid Younesi, Mehdi Yousefi
CD4+CD25+ regulatory T (Treg) cells and Th17 cells play important roles in peripheral immunity. Immune responses are main elements in the pathogenesis of ischemic stroke (IS). The contribution of Th17 cells in IS patients has not been proved, and whether the balance of Treg/Th17 cells is changed in IS patients remains unidentified. In the present study, we studied Th17 and Treg cell frequency, cytokine secretion, expression of transcription factors, and microRNAs related to Th17 and Treg cells differentiation, which is compared between IS patients and control group...
January 20, 2018: Neurological Sciences
https://www.readbyqxmd.com/read/29353313/general-solution-of-the-chemical-master-equation-and-modality-of-marginal-distributions-for-hierarchic-first-order-reaction-networks
#5
Matthias Reis, Justus A Kromer, Edda Klipp
Multimodality is a phenomenon which complicates the analysis of statistical data based exclusively on mean and variance. Here, we present criteria for multimodality in hierarchic first-order reaction networks, consisting of catalytic and splitting reactions. Those networks are characterized by independent and dependent subnetworks. First, we prove the general solvability of the Chemical Master Equation (CME) for this type of reaction network and thereby extend the class of solvable CME's. Our general solution is analytical in the sense that it allows for a detailed analysis of its statistical properties...
January 20, 2018: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/29353155/isolation-purification-structural-characterization-and-immunostimulatory-activity-of-water-soluble-polysaccharides-from-lepidium-meyenii
#6
Zhengqi Zha, Su-Yan Wang, Weihua Chu, Yang Lv, Hongjin Kan, Qiuli Chen, Lili Zhong, Long Yue, Jinna Xiao, Ying Wang, Hongping Yin
A water-soluble polysaccharide LMP-1 was isolated and purified by ion-exchange chromatography from maca (Lepidium meyenii Walp.). LMP-1 has a molecular weight of 1.01 × 104 Da, and is composed of glucose and arabinose with a molar ratio of 7.03:1.08. Methylation and the 1D and 2D NMR spectroscopy of LMP-1 revealed that it is mainly composed of →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Glcp-(1→, and β-D-Araf-(1→, with branching at O-6 of →4,6)-α-D-Glcp-(1 → . LMP-1 showed up-regulation of Toll-like receptor 4 (TLR4) and Toll-like receptor 2 (TLR2)...
January 17, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29353135/copper-induces-expression-and-methylation-changes-of-early-development-genes-in-crassostrea-gigas-embryos
#7
Rossana Sussarellu, Morgane Lebreton, Julien Rouxel, Farida Akcha, Guillaume Rivière
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development...
January 3, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29353102/aging-african-green-monkeys-manifest-transcriptional-pathological-and-cognitive-hallmarks-of-human-alzheimer-s-disease
#8
Paige E Cramer, Renee C Gentzel, Keith Q Tanis, Joshua Vardigan, Yi Wang, Brett Connolly, Philip Manfre, Kenneth Lodge, John J Renger, Celina Zerbinatti, Jason M Uslaner
While many preclinical models of Alzheimer's disease (AD) have been reported, none fully recapitulate the disease. In an effort to identify an appropriate preclinical disease model, we characterized age-related changes in 2 higher order species, the African green monkey (AGM) and the rhesus macaque. Gene expression profiles in the dorsolateral prefrontal cortex and the visual cortex showed age-related changes in AGMs that are strikingly reminiscent of AD, whereas aged rhesus were most similar to healthy elderly humans...
December 20, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/29353080/atlantic-salmon-adapted-to-seawater-for-9-weeks-develop-a-robust-immune-response-to-salmonid-alphavirus-upon-bath-challenge
#9
L J Moore, J Jarungsriapisit, T O Nilsen, S Stefansson, G L Taranger, C J Secombes, H C Morton, S Patel
Pancreas disease (PD) caused by salmonid alphavirus (SAV) is the most serious viral disease in Norwegian aquaculture. Study of the immune response to SAV will aid preventative measures including vaccine development. The innate immune response was studied in Atlantic salmon infected by either bath immersion (BI) or by intra-muscular (i.m.) injection (IM) with SAV subtype 3, two and nine weeks after seawater transfer (Phases A and B respectively). Phase A results have been previously published (Moore et al., 2017) and Phase B results are presented here together with a comparison of results achieved in Phase A...
January 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29353073/serum-hbv-dna-plus-rna-shows-superiority-in-reflecting-the-activity-of-intrahepatic-cccdna-in-treatment-na%C3%A3-ve-hbv-infected-individuals
#10
Hongxin Huang, Jie Wang, Weijie Li, Ran Chen, Xiangmei Chen, Fengmin Zhang, Dongping Xu, Fengmin Lu
BACKGROUND: Both serum hepatitis B virus (HBV) DNA and RNA can reflect intrahepatic covalently closed circular DNA (cccDNA) activity. However, correlations among viral markers haven't been fully explored. OBJECTIVES: Here we investigated the correlations between serum HBV RNA and other viral markers in acute hepatitis B patients and treatment-naïve chronic HBV-infected individuals. STUDY DESIGN: The serum viral markers of 19 acute hepatitis B patients and 84 treatment-naïve chronic HBV-infected individuals at different infection stages were quantified...
January 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29353060/parathyroid-hormone-related-protein-contributes-to-early-healing-of-habu-snakevenom-induced-glomerulonephritis-in-mice
#11
Mazène Hochane, Denis Raison, Catherine Coquard, Claire Béraud, Sabrina Danilin, Audrey Bethry, Thierry Massfelder, Mariette Barthelmebs
Proliferative glomerulonephritis is characterized by local inflammation and mesangial cell deterioration, followed by mesangial proliferation and glomerular healing. Parathyroid-hormone-related peptide (PTHrP) is a mesangial cytokine-like growth factor implicated in mesangial proliferation and survival. No data are available about its role in glomerulonephritis. Herein, we analyzed the expression and role of PTHrP in glomerular inflammation and healing in an experimental model of glomerulonephritis induced by intravenous injection of Habu-snake venom in mice...
January 15, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29353040/eupatilin-an-activator-of-ppar%C3%AE-inhibits-the-development-of-oxazolone-induced-atopic-dermatitis-symptoms-in-balb-c-mice
#12
Yujung Jung, Jin-Chul Kim, No-June Park, Sim-Kyu Bong, Sullim Lee, Hyun Jegal, Li Tai Jin, Sang Moo Kim, Yong Kee Kim, Su-Nam Kim
Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is the main lipophilic flavonoid obtained from the Artemisia species. Eupatilin has been reported to have anti-apoptotic, anti-oxidative and anti-inflammatory activities. Previously, we found that eupatilin increases transcriptional activity and expression of peroxisome proliferator-activated receptor α (PPARα) in a keratinocyte cell line and acts as an agonist of PPARα. PPARα agonists ameliorate atopic dermatitis (AD) and restore the skin barrier function...
January 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29353021/phosphoproteomics-unveils-stable-energy-supply-as-key-to-flooding-tolerance-in-kandelia-candel
#13
Dezhuo Pan, Lingxia Wang, Fanglin Tan, Si Lu, Xiaojie Lv, Madiha Zaynab, Chi-Lien Cheng, Yakubu Saddeeq Abubakar, Shipin Chen, Wei Chen
The mangrove Kandelia candel (L.) Druce experiences daily flooding cycles. To explore the molecular mechanism underlying the physiological adaptation of K. candel to flooding, the potential role of protein phosphorylation in flooding responses was investigated by a large-scale quantitative phosphoproteomic analysis using isobaric tag for relative and absolute quantitation. Total 2141 unique phosphopeptides and 2603 non-redundant phosphorylation sites were identified from 1516 phosphoproteins in K. candel leaves...
January 15, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29353019/induction-by-innate-defence-regulator-peptide-1018-of-pro-angiogenic-molecules-and-endothelial-cell-migration-in-a-high-glucose-environment
#14
Paulina Marin-Luevano, Valentin Trujillo, Adrian Rodriguez-Carlos, Irma González-Curiel, Jose A Enciso-Moreno, Robert E W Hancock, Bruno Rivas-Santiago
Synthetic innate defence regulator (IDR) peptides such as IDR-1018 modulate immunity to promote key protective functions including chemotaxis, wound healing, and anti-infective activity, while suppressing pro-inflammatory responses to non-pathological levels. Here we demonstrated that IDR-1018 induced, by up to 75-fold, pro-angiogenic VEGF-165 in keratinocytes but suppressed this isoform in endothelial cells. It also induced early angiogenin and prolonged anti-inflammatory TGFβ expression on endothelial cells, while suppressing early pro-inflammatory IL-1β expression levels...
January 15, 2018: Peptides
https://www.readbyqxmd.com/read/29352987/the-interaction-between-xbp1-and-enos-contributes-to-endothelial-cell-migration
#15
Junyao Yang, Jing Xu, Martin Danniel, Xiaocong Wang, Wen Wang, Lingfang Zeng, Lisong Shen
The X-box binding protein 1 (XBP1) is a pivotal transcription factor in the endoplasmic reticulum stress response. Our previous studies have proven that XBP1 is involved in vascular endothelial growth factor (VEGF)-mediated endothelial cell (EC) proliferation and angiogenesis. In this study, we used EC monolayer wound healing, tube formation and transwell migration models to explorethe role of XBP1splicing in EC migration. We found that scratching on EC monolayer triggered XBP1splicing, which was attenuated by the presence of SU5416and LY294002, suggesting that VEGF signalling pathways may be involved...
January 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29352975/pinocembrin-from-penthorum-chinense-pursh-suppresses-hepatic-stellate-cells-activation-through-a-unified-sirt3-tgf-%C3%AE-smad-signaling-pathway
#16
Fayang Zhou, Anqi Wang, Dan Li, Yitao Wang, Ligen Lin
The inactivation of hepatic stellate cells (HSCs) has been verified to be an effective therapeutic strategy for treatment of liver fibrosis. Penthorum chinense Pursh has been widely used to protect liver in China; while, the role of P. chinense Pursh in treatment of liver fibrosis is still unexplored. In the current study, the aqueous extract of P. chinense Pursh (PCE) was found to suppress the expressions of fibrotic markers, including collagen I and α-smooth muscle actin (α-SMA), in human HSCs (LX-2); and its major active constituent, pinocembrin (PIN), was discovered to inhibit the expressions of fibrotic markers in LX-2 cells and rat HSCs (HSC-T6)...
January 15, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29352962/improved-systemic-metabolism-and-adipocyte-biology-in-mir-150-knockout-mice
#17
Minsung Kang, Xiaobing Liu, Yuchang Fu, W Timothy Garvey
INTRODUCTION: Short non-coding micro-RNAs (miRNAs) are post-transcriptional factors that directly regulate protein expression by degrading or inhibiting target mRNAs; however, the role of miRNAs in obesity and cardiometabolic disease remains unclarified. Based on our earlier study demonstrating that miR-150 influences lipid metabolism, we have studied effects of miR-150 on systemic metabolism and adipocyte biology. MATERIALS AND METHODS: Metabolic phenotypes including body weight, food intake, body composition, glucose tolerance and insulin sensitivity were assessed in WT and global miR-150 KO male mice fed a high-fat diet...
January 15, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29352846/the-janus-kinase-inhibitor-tofacitinib-inhibits-tnf-%C3%AE-induced-gliostatin-expression-in-rheumatoid-fibroblast-like-synoviocytes
#18
Yohei Kawaguchi, Yuko Waguri-Nagaya, Naoe Tatematsu, Yusuke Oguri, Masaaki Kobayashi, Masahiro Nozaki, Kiyofumi Asai, Mineyoshi Aoyama, Takanobu Otsuka
OBJECTIVES: Gliostatin (GLS) is known to have angiogenic and arthritogenic activity, and GLS expression levels in serum from patients with rheumatoid arthritis (RA) are significantly correlated with the disease activity. Tofacitinib is a novel oral Janus kinase (JAK) inhibitor and is effective in treating RA. However, the mechanism of action of tofacitinib in fibroblast-like synoviocytes (FLSs) has not been elucidated. The purpose of this study was to investigate the modulatory effects of tofacitinib on serum GLS levels in patients with RA and GLS production in FLSs derived from patients with RA...
January 15, 2018: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29352746/the-common-bean-cok-4-and-the-arabidopsis-fer-kinase-domain-share-similar-function-in-plant-growth-and-defense
#19
Rafhael Felipin Azevedo, Maria Celeste Gonçalves Vidigal, Paula Rodrigues Oblessuc, Maeli Melotto
Receptor-like kinases are membrane proteins that can be shared by diverse signaling pathways. Among them, the Arabidopsis thaliana FERONIA (FER) plays a role in the balance between distinct signals to control growth and defense. We have found that COK-4, a putative kinase encoded in the common bean anthracnose resistance locus Co-4 that is transcriptionally regulated during immune response, is highly similar to the kinase domain of FER. To assess whether COK-4 is a functional ortholog of FER, we expressed COK-4 in the wild type Col-0 and the fer-5 mutant of Arabidopsis and evaluated FER-associated traits...
January 20, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29352642/lgals4-ceacam6-tspan8-and-col1a2-blood-markers-for-colorectal-cancer-validation-in-a-cohort-of-subjects-with-positive-fecal-immunochemical-test-result
#20
Maria Teresa Rodia, Rossella Solmi, Francesco Pasini, Elena Nardi, Gabriella Mattei, Giampaolo Ugolini, Luigi Ricciardiello, Pierluigi Strippoli, Rossella Miglio, Mattia Lauriola
BACKGROUND: A noninvasive blood test for the early detection of colorectal cancer (CRC) is highly required. We evaluated a panel of 4 mRNAs as putative markers of CRC. MATERIALS AND METHODS: We tested LGALS4, CEACAM6, TSPAN8, and COL1A2, referred to as the CELTiC panel, using quantitative reverse transcription polymerase chain reaction, on subjects with positive fecal immunochemical test (FIT) results and undergoing colonoscopy. Using a nonparametric test and multinomial logistic model, FIT-positive subjects were compared with CRC patients and healthy individuals...
December 12, 2017: Clinical Colorectal Cancer
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