Jessica Lopes Nobre, Patricia Cristina Lisboa, Janaine Cavalcanti Carvalho, Mariana Renovato Martins, Simone Vargas, Cristina Barja-Fidalgo, Egberto Gaspar de Moura, Elaine de Oliveira
Recently, we demonstrated high serum leptin and 25(OH)D (calcidiol) in obese animals, with high C/EBPβ and PPARγ expression in adipose tissue. Since the role of vitamin D in adipogenesis remains controversial and hyperleptinemia is found in obesity, we asked if leptin could interfere in vitamin D action on adipocytes. Here, we studied the direct effect of these two hormones upon 3T3L1 preadipocytes incubated with or without 1,25(OH)2D (100 nM, 24 h) and with leptin (10-7 M, 4 h later). RT-PCR (VDR and Cyp27b1/1α-hydroxylase), western blotting (VDR, Cyp27b1/1α-hydroxylase, ObR-b, C/EBPβ , PPARγ and Bax content), a cell proliferation assay and an Annexin V-FITC binding assay were performed...
January 12, 2018: General and Comparative Endocrinology