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Cd73

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https://www.readbyqxmd.com/read/28432149/cd73-on-t-cells-orchestrates-cardiac-wound-healing-after-myocardial-infarction-by-purinergic-metabolic-reprogramming
#1
Nadine Borg, Christina Alter, Nicole Görldt, Christoph Jacoby, Zhaoping Ding, Bodo Steckel, Christine Quast, Florian Bönner, Daniela Friebe, Sebastian Temme, Ulrich Flögel, Jürgen Schrader
Background -T-cells are required for proper healing after myocardial infarction. The mechanism of their beneficial action, however, is unknown. The pro-inflammatory "danger signal" adenosine triphosphate (ATP), released from damaged cells, is degraded by the ectonucleotidases CD39 and CD73 to the anti-inflammatory mediator adenosine. Here, we investigate the contribution of CD73-derived adenosine produced by T-cells to cardiac remodeling after ischemia/reperfusion and define its mechanism of action. Methods -Myocardial ischemia (50 min /reperfusion) was induced in global CD73(-/-) and CD4-CD73(-/-) mice...
April 21, 2017: Circulation
https://www.readbyqxmd.com/read/28431407/prognositic-value-of-cd73-adenosinergic-pathway-in-solid-tumor-a-meta-analysis-and-systematic-review
#2
Rong Wang, Yingying Zhang, Xia Lin, Yalin Gao, Ying Zhu
CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein that is encoded by NT5E gene, plays multiple roles in tumor processes. Previous studies have presented a potential value of CD73 served as a detectable biomarker for prognosis of several solid tumors, but the results were more controversially. A comprehensive meta-analysis was conducted to precisely evaluate the prognostic role of CD73 in solid tumors. The included studies were searched in PubMed, Web of Science and EBSCO from Jan 1990 to Jan 2016...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28410670/characterisation-and-intracellular-labelling-of-mesenchymal-stromal-cells-derived-from-synovial-fluid-of-horses-and-sheep
#3
J Burk, S M Glauche, W Brehm, A Crovace, E Francioso, A Hillmann, S Schubert, L Lacitignola
Multipotent mesenchymal stromal cells (MSCs) derived from synovial fluid (SF) are considered to be a promising cell type for therapeutic applications in joint disease. However, despite their potential relevance for clinical and experimental studies, there is insufficient knowledge about SF-derived MSCs isolated from horses and sheep. In this study, cells were recovered from healthy SF and bone marrow (BM) of sheep, and from healthy and osteoarthritic SF of horses. Ovine SF-MSCs were used to assess the efficiency of intracellular labelling with quantum dots (QDs)...
April 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28409292/effects-of-the-donor-age-on-proliferation-senescence-and-osteogenic-capacity-of-human-urine-derived-stem-cells
#4
Peng Gao, Peilin Han, Dapeng Jiang, Shulong Yang, Qingbo Cui, Zhaozhu Li
To study the effects of the donor age on the application potential of human urine-derived stem cells (hUSCs) in bone tissue engineering, by comparing proliferation, senescence and osteogenic differentiation of hUSCs originated from volunteers with different ages. The urine samples were collected from 19 healthy volunteers (6 cases from children group aged from 5 to 14, 5 cases from middle-aged group aged from 30 to 40, and 8 cases from the elder group aged from 65 to 75), and hUSCs were isolated and cultured...
April 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28408751/mmp9-integrates-multiple-immunoregulatory-pathways-that-discriminate-high-suppressive-activity-of-human-mesenchymal-stem-cells
#5
Carolina Lavini-Ramos, Hernandez Moura Silva, Alessandra Soares-Schanoski, Sandra Maria Monteiro, Ludmila Rodrigues Pinto Ferreira, Ana Paula Pacanaro, Samirah Gomes, Janaína Batista, Kellen Faé, Jorge Kalil, Verônica Coelho
The mechanisms underlying mesenchymal stem cells' (MSC) suppressive potency are largely unknown. We here show that highly suppressive human adipose tissue-derived MSC (AdMSC) display and induce a differential immunologic profile, upon ongoing AdMSC suppressive activity, promoting: (i) early correlated inhibition of IFN-γ and TNF-α production, along IL-10 increase, (ii) CD73(+)Foxp3(+)Treg subset expansion, and (iii) specific correlations between gene expression increases, such as: MMP9 correlated with CCL22, TNF, FASL, RUNX3, and SEMAD4 in AdMSC and, in T cells, MMP9 upregulation correlated with CCR4, IL4 and TBX21, among others, whereas MMP2 correlated with BCL2 and LRRC31...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28404888/ecto-5-nucleotidase-cd73-is-a-biomarker-for-clear-cell-renal-carcinoma-stem-like-cells
#6
Lei Song, Wenling Ye, Yong Cui, Jianzhong Lu, Yanan Zhang, Nan Ding, Wentao Hu, Hailong Pei, Zhongjin Yue, Guangming Zhou
Identification of a specific biomarker for cancer stem cells (CSCs) is of potential applications in the development of effective therapeutic strategies for renal cell carcinoma (RCC). In this study, both the RCC cell line 786-O and surgically removed clear cell RCC (ccRCC) tissues were implemented to grew as spheroids in serum-free medium supplemented with mitogens. This subpopulation possessed key characteristics defining CSCs. We also identified that surgically removed ccRCC tissues were heterogenic and there was a subpopulation of cells that was highly stained with rhodamine-123...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28404626/adenosine-formed-by-cd73-on-t-cells-inhibits-cardiac-inflammation-and-fibrosis-and-preserves-contractile-function-in-transverse-aortic-constriction-induced-heart-failure
#7
Christine Quast, Christina Alter, Zhaoping Ding, Nadine Borg, Jürgen Schrader
BACKGROUND: Structural damage during heart failure development leads to increased infiltration of leukocytes. Because purinergic signaling on immune cells may impact on the inflammatory response, we evaluated the role of ecto-5'-nucleotidase (CD73) on the development of heart failure after transverse aortic constriction (TAC) using global and T-cell-specific CD73(-)(/-) mice. METHODS AND RESULTS: Leukocytes infiltrating the failing heart were analyzed by a multistep enzymatic procedure over a period of 16 weeks using fluorescence-activated cell sorting...
April 2017: Circulation. Heart Failure
https://www.readbyqxmd.com/read/28399915/tiamulin-inhibits-breast-cancer-growth-and-pulmonary-metastasis-by-decreasing-the-activity-of-cd73
#8
Xu Yang, Shimin Pei, Huanan Wang, Yipeng Jin, Fang Yu, Bin Zhou, Hong Zhang, Di Zhang, Degui Lin
BACKGROUND: Metastasis is the leading cause of death in breast cancer patients. CD73, also known as ecto-5'-nucleotidase, plays a critical role in cancer development including metastasis. The existing researches indicate that overexpression of CD73 promotes growth and metastasis of breast cancer. Therefore, CD73 inhibitor can offer a promising treatment for breast cancer. Here, we determined whether tiamulin, which was found to inhibit CD73, was able to suppress breast cancer development and explored the related mechanisms...
April 11, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28399672/targeting-adenosine-in-cancer-immunotherapy-a-review-of-recent-progress
#9
Theresa L Whiteside
The adenosine pathway plays a key role in modulating immune responses in health and in disease. In health, anti-inflammatory effects of adenosine balance pro-inflammatory ATP, limiting tissue destruction by activated immune cells. In disease, this balance is disturbed. Areas covered: This review focuses on cancer and explains how in the microenvironment, the ATP-adenosine balance shifts towards an excess of extracellular adenosine Expert commentary: The CD73-adenosine axis plays a key role in the inhibition of anti-tumor functions of immune effector cells...
April 12, 2017: Expert Review of Anticancer Therapy
https://www.readbyqxmd.com/read/28397110/potential-role-of-p2x7r-in-esophageal-squamous-cell-carcinoma-proliferation
#10
André A Santos, Angélica R Cappellari, Fernanda O de Marchi, Marina P Gehring, Aline Zaparte, Caroline A Brandão, Tiago Giuliani Lopes, Vinicius D da Silva, Luis Felipe Ribeiro Pinto, Luiz Eduardo Baggio Savio, Aline Cristina Abreu Moreira-Souza, Robson Coutinho-Silva, Juliano D Paccez, Luiz F Zerbini, Fernanda B Morrone
Esophageal cancer is an aggressive tumor and is the sixth leading cause of cancer death worldwide. ATP is well known to regulate cancer progression in a variety of models by different mechanisms, including P2X7R activation. This study aimed to evaluate the role of P2X7R in esophageal squamous cell carcinoma (ESCC) proliferation. Our results show that treatment with high ATP concentrations induced a decrease in cell number, cell viability, number of polyclonal colonies, and reduced migration of ESCC. The treatment with the selective P2X7R antagonist A740003 or siRNA for P2X7 reverted this effect in the KYSE450 cell line...
April 11, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28383711/synergistic-effect-of-regulatory-t-cells-and-proinflammatory-cytokines-in-angiogenesis-in-the-endometriotic-milieu
#11
Xiao-Qiu Wang, Wen-Jie Zhou, Xue-Zhen Luo, Yu Tao, Da-Jin Li
STUDY QUESTION: Do regulatory T cells (Tregs) contribute to angiogenesis in endometriosis? SUMMARY ANSWER: High levels of CCL17 and CCL22 cause the recruitment of Tregs, upregulate the immunosuppression of Tregs and, in turn, may promote angiogenesis in endometrial cells in synergy with proinflammatory cytokines. WHAT IS ALREADY KNOWN: The peritoneal fluid of patients with endometriosis has a higher percentage of Tregs than that of normal individuals; however, the regulatory role of Tregs in the disease remains unclear...
April 4, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28377454/cellular-prion-protein-prpc-and-ecto-5-nucleotidase-are-markers-of-the-cellular-stress-response-to-aneuploidy
#12
Patrícia Henriques Domingues, Lalitha S Y Nanduri, Katarzyna Seget, Sharavan V Venkateswaran, David Agorku, Cristina Viganó, Conrad von Schubert, Erich A Nigg, Charles Swanton, Rocio Sotillo, Andreas Bosio, Zuzana Storchová, Olaf Hardt
Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of ~300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PRPC and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73...
April 4, 2017: Cancer Research
https://www.readbyqxmd.com/read/28377320/do-il-3-gm-csf-effect-on-the-myofibroblastic-differentiation-of-human-adipose-derived-stromal-cells
#13
Jae-Sun Lee, Son Seung Tae, Deok-Yeol Kim, Seung-Kyu Han, Woo-Kyung Kim, Eun-Sang Dhong
BACKGROUND: Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast after the treatment of inflammatory cytokines in vitro. MATERIALS AND METHODS: Interleukin 3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF) were treated in the culture of hASCs and HDFs...
April 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28376684/equine-mesenchymal-stromal-cells-retain-a-pericyte-like-phenotype
#14
Cristina L Esteves, Tara A Sheldrake, Lucy Dawson, Timothy Menghini, Elisabeth Rink, Karin Amilon, Nusrat Khan, Bruno Peault, Xavier Donadeu
Mesenchymal Stem/Stromal Cells (MSCs) have been used in human and equine regenerative medicine, and interest in exploiting their potential has increased dramatically over the years. Despite significant effort to characterize equine MSCs, the actual origin of these cells and how much of their native phenotype is maintained in culture have not been determined. In this study, we investigated the relationship between MSCs, derived from adipose tissue (AT) and bone marrow (BM), and pericytes in the horse. Both pericyte (CD146, NG2 and αSMA) and MSC (CD29, CD90 and CD73) markers were detected in equine adipose tissue and co-localized around blood vessels...
April 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28376099/the-adenosine-generating-enzymes-cd39-cd73-control-microglial-processes-ramification-in-the-mouse-brain
#15
Marina Matyash, Oleksandr Zabiegalov, Stefan Wendt, Vitali Matyash, Helmut Kettenmann
Microglial cells invade the brain as amoeboid precursors and acquire a highly ramified morphology in the postnatal brain. Microglia express all essential purinergic elements such as receptors, nucleoside transporters and ecto-enzymes, including CD39 (NTPDase1) and CD73 (5'-nucleotidase), which sequentially degrade extracellular ATP to adenosine. Here, we show that constitutive deletion of CD39 and CD73 or both caused an inhibition of the microglia ramified phenotype in the brain with a reduction in the length of processes, branching frequency and number of intersections with Sholl spheres...
2017: PloS One
https://www.readbyqxmd.com/read/28375551/phenotypic-characterization-of-regulatory-t-cells-from-antiretroviral-na%C3%A3-ve-hiv-1-infected-people
#16
Georgia N Ambada, Claudine E Ntsama, Nadesh N Nji, Loveline N Ngu, Sake N Carole, Abel Lissom, Flaurent T Tchouangeu, Jules Tchadji, Martin Sosso, François-Xavier Etoa, Godwin W Nchinda
Regulatory T cells (Tregs) play a key role in dampening excessive immune activation. However antiretroviral (ARV) naive HIV-1 infection maintains the immune system in a sustained state of activation that could alter both Tregs surface markers and functions. Since Tregs surface markers are directly linked to their functions the overall objective of this study was to determine how ARV naïve HIV infection affects the phenotypic properties of Tregs. Our data showed that in ARV naive HIV-1 infection, Tregs are dominated by effector (CD45RA(+) CD27(-) CCR7(-) CD62L(-) ) and effector memory (CD45RA(-) CD27(-) CCR7(-) CD62L(-) ) cells...
April 4, 2017: Immunology
https://www.readbyqxmd.com/read/28373875/roles-and-modalities-of-ectonucleotidases-in-remodeling-the-multiple-myeloma-niche
#17
Antonella Chillemi, Valeria Quarona, Luca Antonioli, Davide Ferrari, Alberto L Horenstein, Fabio Malavasi
Ectoenzymes are cell surface molecules, which represent functional bridges between the environment and the cytoplasm. One set of ectoenzymes-CD39, CD38, CD203a, and CD73-leads to the generation of adenosine (ADO) by metabolizing ATP and NAD(+). While ADO is known to control inflammation and suppress immune responses, other aspects of ADO function are still obscure, mainly due to its short half-life in biological fluids. Human multiple myeloma (MM) grows in the closed system of the bone marrow (BM) niche representing an ideal setting for studying ectoenzymes and their products...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28373446/alpha-bisabolol-promotes-glioma-cell-death-by-modulating-the-adenosinergic-system
#18
Franciane Brackmann Mendes, Letícia Scussel Bergamin, Cristiane Dos Santos Stuepp, Elizandra Braganhol, Thatiana Terroso, Adriana Rafin Pohlmann, Sílvia Stanisçuaski Guterres, Ana Maria Oliveira Battastini
BACKGROUND/AIM: Glioblastoma multiforme is the most malignant type of glioma. Alpha-bisabolol is an essential oil reported as a potent cell death agent. In the present work, we evaluated the effect of alpha-bisabolol on ecto-5'-nucleotidase/CD73, the most well-characterized enzymatic source of adenosine, present in lipid rafts. MATERIALS AND METHODS: Glioma cells were treated with alpha-bisabolol and, in some experiments, pre-treated with an A3 antagonist. MTT assay (viability), malachite green method (ecto-5'-nucleotidase/CD73 activity) and quantitative polymerase chain reaction (qPCR) (A3 mRNA) were carried out...
April 2017: Anticancer Research
https://www.readbyqxmd.com/read/28366194/effects-of-a-defined-xeno-free-medium-on-the-growth-and-neurotrophic-and-angiogenic-properties-of-human-adult-stem-cells
#19
Maria Brohlin, Peyman Kelk, Mikael Wiberg, Paul J Kingham
BACKGROUND: The growth properties and neurotrophic and angiogenic effects of human mesenchymal stromal cells (MSCs) cultured in a defined xeno-free, serum-free medium (MesenCult-XF) were investigated. METHODS: Human MSCs from adipose tissue (ASCs) and bone marrow (BMSCs) were cultured in Minimum Essential Medium-alpha (α-MEM) containing fetal calf serum or in MesenCult-XF. Proliferation was measured over 10 passages and the colony-forming unit (CFU) assay and expression of cluster of differentiation (CD) surface markers were determined...
May 2017: Cytotherapy
https://www.readbyqxmd.com/read/28363952/cd73-derived-adenosine-and-tenascin-c-control-cytokine-production-by-epicardium-derived-cells-formed-after-myocardial-infarction
#20
Julia Hesse, Stella Leberling, Elisabeth Boden, Daniela Friebe, Timo Schmidt, Zhaoping Ding, Peter Dieterich, Andreas Deussen, Claudia Roderigo, Christine R Rose, Doreen M Floss, Jürgen Scheller, Jürgen Schrader
Epicardium-derived cells (EPDCs) play a fundamental role in embryonic cardiac development and are reactivated in the adult heart in response to myocardial infarction (MI). In this study, EPDCs from post-MI rat hearts highly expressed the ectoenzyme CD73 and secreted the profibrotic matricellular protein tenascin-C (TNC). CD73 on EPDCs extensively generated adenosine from both extracellular ATP and NAD. This in turn stimulated the release of additional nucleotides from a Brefeldin A-sensitive intracellular pool via adenosine-A2BR signaling, forming a positive-feedback loop...
March 31, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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