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https://www.readbyqxmd.com/read/28816507/arthroscopic-harvest-of-adipose-derived-mesenchymal-stem-cells-from-the-infrapatellar-fat-pad
#1
Jason L Dragoo, Wenteh Chang
BACKGROUND: The successful isolation of adipose-derived mesenchymal stem cells (ADSCs) from the arthroscopically harvested infrapatellar fat pad (IFP) would provide orthopaedic surgeons with an autologous solution for regenerative procedures. PURPOSE: To demonstrate the quantity and viability of the mesenchymal stem cell population arthroscopically harvested from the IFP as well as the surrounding synovium. STUDY DESIGN: Descriptive laboratory study...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28816410/new-insights-into-the-cellular-makeup-and-progenitor-potential-of-palatal-connective-tissues
#2
Emoke Pall, Mihai Cenariu, Adrian Kasaj, Adrian Florea, Andrada Soancă, Alexandra Roman, Carmen Georgiu
The present study investigated the regenerative potential of connective tissues harvested from two palatal areas widely used as donor sites for muco-gingival surgical approaches. Connective tissue grafts (CTGs) were obtained by de-epithelialisation of a free gingival graft (deCTG) and by a split flap approach from a previous donor site (reCTG). Two types of mesenchymal stem cell (MSCs) were isolated and were named de-epithelialised MSCs (deMSCs) and re-entry MSCs (reMSCs). The cells were characterised and cellular functionality was investigated...
August 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28805694/progenitor-cells-from-cartilage-grade-specific-differences-in-stem-cell-marker-expression
#3
Marija Mazor, Annabelle Cesaro, Mazen Ali, Thomas M Best, Eric Lespessaille, Hechmi Toumi
Recent research has confirmed the presence of Mesenchymal stem cell (MSC)-like progenitors (MPC) in both normal and osteoarthritic cartilage. However, there is only limited information concerning how MPC markers are expressed with osteoarthritis (OA) progression. The purpose of this study was to compare the prevalence of various MPC markers in different OA grades. Human osteoarthritic tibial plateaus were obtained from ten patients undergoing total knee replacement. Each sample had been classified into a mild or severe group according to OARSI scoring...
August 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28796790/mesenchymal-stromal-cells-msc-from-jak2-myeloproliferative-neoplasms-differ-from-normal-msc-and-contribute-to-the-maintenance-of-neoplastic-hematopoiesis
#4
Teresa L Ramos, Luis Ignacio Sánchez-Abarca, Beatriz Rosón-Burgo, Alba Redondo, Ana Rico, Silvia Preciado, Rebeca Ortega, Concepción Rodríguez, Sandra Muntión, Ángel Hernández-Hernández, Javier De Las Rivas, Marcos González, José Ramón González Porras, Consuelo Del Cañizo, Fermín Sánchez-Guijo
There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HD-MSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls...
2017: PloS One
https://www.readbyqxmd.com/read/28777945/long-term-engraftment-of-primary-bone-marrow-stromal-cells-repairs-niche-damage-and-improves-hematopoietic-stem-cell-transplantation
#5
Jean-Paul Abbuehl, Zuzana Tatarova, Werner Held, Joerg Huelsken
Hematopoietic stem cell (HSC) transplantation represents a curative treatment for various hematological disorders. However, delayed reconstitution of innate and adaptive immunity often causes fatal complications. HSC maintenance and lineage differentiation are supported by stromal niches, and we now find that bone marrow stroma cells (BMSCs) are severely and permanently damaged by the pre-conditioning irradiation required for efficient HSC transplantation. Using mouse models, we show that stromal insufficiency limits the number of donor-derived HSCs and B lymphopoiesis...
August 3, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28775208/a-multikinase-and-dna-pk-inhibitor-combination-immunomodulates-melanomas-suppresses-tumor-progression-and-enhances-immunotherapies
#6
Alexander K Tsai, Asra Y Khan, Christina E Worgo, Lucy L Wang, Yuanyuan Liang, Eduardo Davila
Combination therapies have the potential to improve outcomes in melanoma patients but have not yet been clinically efficacious. Here, we used high-throughput flow cytometry-based screening to identify and characterize candidate therapies that might synergize with and augment T-cell immunotherapy efficacy. Two lead therapies, regorafenib and NU7441, were selected based on their ability to alter a variety of immunomodulatory proteins, including CD55, CD73, CD155, programmed death-ligand 1 (PD-L1), nerve growth factor receptor (NGFR), and HLA class I in a heterogeneous panel of melanomas...
August 3, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28773947/behavior-of-human-bone-marrow-derived-mesenchymal-stem-cells-on-various-titanium-based-coatings
#7
Chengjuan Qu, Salla Kaitainen, Heikki Kröger, Reijo Lappalainen, Mikko J Lammi
The chemical composition and texture of titanium coatings can influence the growth characteristics of the adhered cells. An enhanced proliferation of the human mesenchymal stem cells (hMSCs) would be beneficial. The present study was aimed to investigate whether titanium deposited at different atmospheres would affect the cell growth properties, cellular morphology, and expression of surface markers of hMSCs. Titanium-based coatings were deposited on silicon wafers under oxygen, nitrogen, or argon atmospheres by ultra-short pulsed laser deposition using two different gas pressures followed by heating at 400 °C for 2 h...
October 12, 2016: Materials
https://www.readbyqxmd.com/read/28766061/lysophosphatidic-acid-enhances-human-umbilical-cord-mesenchymal-stem-cell-viability-without-differentiation-via-lpa-receptor-mediating-manner
#8
Narengerile Li, Ya-Li Yan, Sachaofu Fu, Rui-Juan Li, Peng-Fei Zhao, Xi-Yuan Xu, Jing-Ping Yang, Alatangaole Damirin
Human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential stromal cells which are regarded as the most feasible stem cell group in cell therapy. The maintenance of cell survival without differentiation is important in cell transplantation and stem cell therapy. However, negative factors exist in cell transplantation. Lysophosphatidic acid (LPA) is a non-antigenic small molecule phospholipid which induced several fundamental cellular responses, such as cell proliferation, apoptosis and migration. In this study we aimed to explore the effects of LPA on the survival and differentiation of MSCs and its availability in cell therapy...
August 1, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28762648/directed-differentiation-of-human-embryonic-stem-cells-to-neural-crest-stem-cells-functional-peripheral-neurons-and-corneal-keratocytes
#9
Qian Zhu, Mingming Li, Chuan Yan, Qiqi Lu, Shunhui Wei, Rong Gao, Mengfei Yu, Yu Zou, Gopu Sriram, Huei Jinn Tong, Walter Hunziker, Chaminda Jayampath Seneviratne, Zhiyuan Gong, Bjorn R Olsen, Tong Cao
Neural crest stem cells (NCSCs) are a transient and multipotent cell population giving rise to various cell types with clinical importance. Isolation of human NCSCs is extremely challenging that limits our knowledge about neural crest development and application. Here, we present a defined protocol to efficiently direct human embryonic stem cells (hESCs) to NCSCs and multiple neural crest lineages. A unique combination of small molecule inhibitors and growth factors was employed to generate NCSCs from hESCs through a neuroectoderm stage...
August 1, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28761446/characteristics-of-human-endometrium-derived-mesenchymal-stem-cells-and-their-tropism-to-endometriosis
#10
Yan Cheng, Liru Li, Dejun Wang, Qiuyan Guo, Yanan He, Tian Liang, Liyuan Sun, Xiaojun Wang, Yulei Cheng, Guangmei Zhang
Human endometrial tissue has become an attractive source of mesenchymal stem cells (MSCs) for cell-based therapies because these MSCs can be easily harvested and have tumour tropism as well as reduced immunogenic and inflammatory properties. Our study aimed to obtain and characterise human endometrial mesenchymal stem cells (EMSCs) and assess their endometriosis tropism. EMSCs were successfully isolated from the endometrium of women undergoing laparoscopy for idiopathic infertility. The EMSCs presented a fibroblast-like morphology during culture...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28759195/photoacoustic-imaging-of-human-mesenchymal-stem-cells-labeled-with-prussian-blue-poly-l-lysine-nanocomplexes
#11
Taeho Kim, Jeanne E Lemaster, Fang Chen, Jin Li, Jesse V Jokerst
Acoustic imaging is affordable and accessible without ionizing radiation. Photoacoustic imaging increases the contrast of traditional ultrasound and can offer good spatial resolution when used at high frequencies with excellent temporal resolution. Prussian blue nanoparticles (PBNPs) are an emerging photoacoustic contrast agent with strong optical absorption in the near-infrared region. In this study, we developed a simple and efficient method to label human mesenchymal stem cells (hMSCs) with PBNPs and imaged them with photoacoustic imaging...
August 4, 2017: ACS Nano
https://www.readbyqxmd.com/read/28752609/the-atrial-appendage-as-a-suitable-source-to-generate-cardiac-derived-adherent-proliferating-cells-for-regenerative-cell-based-therapies
#12
Stephan Detert, Christof Stamm, Christien Beez, Falk Diedrichs, Jochen Ringe, Sophie Van Linthout, Martina Seifert, Carsten Tschöpe, Michael Sittinger, Marion Haag
Cardiac-derived adherent proliferating (CardAP) cells obtained from endomyocardial biopsies (EMBs) with known anti-fibrotic and pro-angiogenic properties are good candidates for the autologous therapy of end-stage cardiac diseases such as dilated cardiomyopathy. However, due to the limited number of CardAP cells that can be obtained from EMBs, our aim is to isolate cells with similar properties from other regions of the heart with comparable tissue architecture. Here, we introduce the atrial appendage as a candidate region...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28747757/tgf%C3%AE-induced-osteogenic-potential-of-human-amniotic-fluid-stem-cells-via-cd73-generated-adenosine-production
#13
Kwan-Leong Hau, Anna Maria Ranzoni, Filipa Vlahova, Kate Hawkins, Paolo De Coppi, Anna L David, Pascale V Guillot
The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28746783/cd73-expression-identifies-a-subset-of-igm-antigen-experienced-cells-with-memory-attributes-that-is-t-cell-and-cd40-signaling-dependent
#14
Lucas D'Souza, Sneh Lata Gupta, Vineeta Bal, Satyajit Rath, Anna George
B cell memory was long characterized as isotype-switched, somatically mutated and germinal center (GC)-derived. However, it is now clear that the memory pool is a complex mixture that includes unswitched and unmutated cells. Further, expression of CD73, CD80 and CD273 has allowed the categorization of B cell memory into multiple subsets, with combinatorial expression of the markers increasing with GC progression, isotype-switching and acquisition of somatic mutations. We have extended these findings to determine whether these markers can be used to identify IgM memory phenotypically as arising from T-dependent versus T-independent responses...
July 26, 2017: Immunology
https://www.readbyqxmd.com/read/28746349/new-insights-on-the-regulation-of-the-adenine-nucleotide-pool-of-erythrocytes-in-mouse-models
#15
William G O'Brien, Han Shawn Ling, Zhaoyang Zhao, Cheng Chi Lee
The observation that induced torpor in non-hibernating mammals could result from an increased AMP concentration in circulation led our investigation to reveal that the added AMP altered oxygen transport of erythrocytes. To further study the effect of AMP in regulation of erythrocyte function and systemic metabolism, we generated mouse models deficient in key erythrocyte enzymes in AMP metabolism. We have previously reported altered erythrocyte adenine nucleotide levels corresponding to altered oxygen saturation in mice deficient in both CD73 and AMPD3...
2017: PloS One
https://www.readbyqxmd.com/read/28743609/expression-of-cd73-slows-down-migration-of-skin-dendritic-cells-affecting-the-sensitization-phase-of-contact-hypersensitivity-reactions-in-mice
#16
A Neuberger, S Ring, C Silva-Vilches, J Schrader, A Enk, K Mahnke
BACKGROUND: Application of haptens to the skin induces release of immune stimulatory ATP into the extracellular space. This "danger" signal can be converted to immunosuppressive adenosine (ADO) by the action of the ectonucleotidases CD39 and CD73, expressed by skin and immune cells. Thus, the expression and regulation of CD73 by skin derived cells may have crucial influence on the outcome of contact hypersensitivity (CHS) reactions. OBJECTIVE: To investigate the role of CD73 expression during 2,4,6-trinitrochlorobenzene (TNCB) induced CHS reactions...
July 9, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28728289/-the-protective-effect-of-pigment-epithelial-derived-factor-modified-human-umbilical-cord-mesenchymal-stem-cells-on-rats-with-diabetic-retinopathy
#17
W Zhang, H T Duan, S Chen, Y X Wang, J H Kong, M Dong, X Bi, J Song
Objective: To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Methods: Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28720305/ecto-5-nucleotidase-cd73-regulates-bone-formation-and-remodeling-during-intramembranous-bone-repair-in-aging-mice
#18
Vivian Bradaschia-Correa, Anne M Josephson, Alexander J Egol, Matthew M Mizrahi, Kevin Leclerc, Jason Huo, Bruce N Cronstein, Philipp Leucht
Ecto-5'-nucleotidase (CD73) generates adenosine, an osteoblast activator and key regulator of skeletal growth. It is unknown, however, if CD73 regulates osteogenic differentiation during fracture healing in adulthood, and in particular how CD73 activity regulates intramembranous bone repair in the elderly. Monocortical tibial defects were created in 46-52-week-old wild type (WT) and CD73 knock-out mice (CD73(-/-)) mice. Injury repair was analyzed at post-operative days 5, 7, 14 and 21 by micro-computed tomography (micro-CT), histomorphometry, proliferating cell nuclear antigen (PCNA) immunostaining, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) histochemistry...
July 4, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28717030/intestinal-epithelial-ecto-5-nucleotidase-cd73-regulates-intestinal-colonization-and-infection-by-nontyphoidal-salmonella
#19
Daniel J Kao, Bejan J Saeedi, David Kitzenberg, Krista M Burney, Evgenia Dobrinskikh, Kayla D Battista, Andrés Vázquez-Torres, Sean P Colgan, Douglas J Kominsky
Ecto-5' -nucleotidase (CD73) is expressed abundantly on the apical surface of intestinal epithelial cells (IEC) and functions as the terminal enzyme in the generation of extracellular adenosine. Previous work has demonstrated that adenosine signaling in IECs results in a number of tissue-protective effects during inflammation; however, a rationale for its apical expression has been lacking. We hypothesized that the highly polarized expression of CD73 is indicative of an important role for extracellular adenosine as a mediator of host-microbe interactions...
July 17, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28713938/effects-of-age-on-biological-and-functional-characterization-of-adipose%C3%A2-derived-stem-cells-from-patients-with-end%C3%A2-stage-liver-disease
#20
Yanyu Jin, Lei Yang, Yanyan Zhang, Wei Gao, Zhi Yao, Yang Song, Yuliang Wang
Adipose‑derived mesenchymal stromal cells (ADSCs) possess a multilineage potential and immunoregulatory properties, and may have great potential in autologous cell‑based technologies. The aim of the present study was to investigate how the age of patients with benign end‑stage liver disease affected the biological and functional characteristics of ADSCs, which is important for increasing the potential effectiveness of autologous cell therapy. ADSCs were obtained and cultured from three distinct age groups: Infant, adult and elderly...
September 2017: Molecular Medicine Reports
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