keyword
https://read.qxmd.com/read/38439112/docosahexaenoic-acid-dha-impairs-hypoxia-induced-cellular-and-exosomal-overexpression-of-immune-checkpoints-and-immunomodulatory-molecules-in-different-subtypes-of-breast-cancer-cells
#21
JOURNAL ARTICLE
Sepideh Maralbashi, Cynthia Aslan, Houman Kahroba, Milad Asadi, Mohammad Sadegh Soltani-Zangbar, Navideh Haghnavaz, Farhad Jadidi, Farhad Salari, Tohid Kazemi
BACKGROUND: Tumor cells express immune-checkpoint molecules to suppress anti-tumor immune responses. In part, immune evasion takes place by secreting exosomes bearing immune-checkpoint and immunomodulatory molecules and their inducing and/or regulating agents e.g., microRNAs (miRs). This study aimed to evaluate the effects of omega-3 fatty acid, docosahexaenoic acid (DHA), on the expression of some selected immune-checkpoint and immunomodulatory molecules and their regulating miRs under both normoxic and hypoxic conditions in triple negative (TNBC) invasive and triple positive non-invasive breast cancer cell lines...
March 4, 2024: BMC Nutrition
https://read.qxmd.com/read/38435114/increased-pd-1-nk-cell-subset-in-the-older-population
#22
JOURNAL ARTICLE
Meiju Deng, Yongqin Zeng, Ying Liu, Xiaolei Wang, Na Chen, Mengyuan Zhang, Meiqing Jiang, Hongxin Zhao, Juan Du
BACKGROUND: The aging of the immune system is associated with various diseases. It is worth exploring the changes of the immune system in aging. Previous studies have shown that aged T cells have enhanced expression of co-inhibitory molecules. However, it remains unclear whether aged NK cells exhibit similar characteristics to aged T cells. The objective of our research was to clarify this aspect. PATIENTS AND METHODS: This study included 98 adults aged 24-90 years (50 males and 48 females)...
2024: International Journal of General Medicine
https://read.qxmd.com/read/38434980/causal-relationship-between-immune-cells-and-gynecological-cancers-through-bidirectional-and-multivariable-mendelian-randomization-analyses
#23
JOURNAL ARTICLE
Yangyang Zhang, Yangyuxiao Lu, Xuanyu Wang, Keren He, Mengqi Fang, Jiabao Xu, Ye Xu, Fangfang Tao, Ping Lü
Background: Evidence suggests potential associations between gynecological malignancies and various immune cell chemicals and systems. However, establishing a causal relationship remains uncertain. Methods: This work employed Wald ratio for one single-nucleotide polymorphism (SNP) or the inverse-variance weighted method (IVW) for multiple SNPs to conduct bidirectional two-sample Mendelian randomization (MR) analysis by utilizing genome-wide association study (GWAS) data. We employed supplementary methods, including MR-Egger and weighted median methods, to detect and correct for the influence of horizontal pleiotropy...
2024: Journal of Cancer
https://read.qxmd.com/read/38428703/cd39-hi-identifies-an-exhausted-tumor-reactive-cd8-t-cell-population-associated-with-tumor-progression-in-human-gastric-cancer
#24
JOURNAL ARTICLE
Yang Shen, Yuan Qiu, Zhen-Quan Duan, Yu-Xian Li, Ying Wang, Yuan-Yuan Zhang, Bao-Hang Zhu, Xiao-Hong Yu, Xue-Ling Tan, Weisan Chen, Yuan Zhuang, Quan-Ming Zou, Dai-Yuan Ma, Liu-Sheng Peng
The ectonucleotidase CD39 has been regarded as a promising immune checkpoint in solid tumors. However, the expression of CD39 by tumor-infiltrating CD8+ T cells as well as their potential roles and clinical implications in human gastric cancer (GC) remain largely unknown. Here, we found that GC-infiltrating CD8+ T cells contained a fraction of CD39hi cells that constituted about 6.6% of total CD8+ T cells in tumors. These CD39hi cells enriched for GC-infiltrating CD8+ T cells with features of exhaustion in transcriptional, phenotypic, metabolic and functional profiles...
February 29, 2024: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://read.qxmd.com/read/38408984/causal-effects-between-circulating-immune-cells-and-heart-failure-evidence-from-a-bidirectional-mendelian-randomization-study
#25
JOURNAL ARTICLE
Rutao Bian, Xuegong Xu, Zishuang Li
BACKGROUND: Heart failure (HF) is a prevalent cardiac condition characterized by high mortality and morbidity rates. Immune cells play a pivotal role as crucial biomarkers in assessing the overall immune status of individuals. However, the causal relationship between circulating immune cells and the pathogenesis of HF remains an area requiring further investigation. OBJECTIVES: The aim of this study was to investigate the genetic interactions between circulating immune cells and HF, and to further elucidate the genetic associations between different lymphocyte subsets and HF...
February 26, 2024: BMC Medical Genomics
https://read.qxmd.com/read/38391970/regulatory-b-cells-immunopathological-and-prognostic-potential-in-humans
#26
REVIEW
Johanna Veh, Carolin Ludwig, Hubert Schrezenmeier, Bernd Jahrsdörfer
The aim of the following review is to shed light on the putative role of regulatory B cells (Bregs) in various human diseases and highlight their potential prognostic and therapeutic relevance in humans. Regulatory B cells are a heterogeneous group of B lymphocytes capable of suppressing inflammatory immune reactions. In this way, Bregs contribute to the maintenance of tolerance and immune homeostasis by limiting ongoing immune reactions temporally and spatially. Bregs play an important role in attenuating pathological inflammatory reactions that can be associated with transplant rejection, graft-versus-host disease, autoimmune diseases and allergies but also with infectious, neoplastic and metabolic diseases...
February 18, 2024: Cells
https://read.qxmd.com/read/38367982/adenosine-metabolic-signature-in-circulating-cd4-t-cells-predicts-remission-in-rheumatoid-arthritis
#27
JOURNAL ARTICLE
Philip M Brown, Amy E Anderson, Najib Naamane, Dennis W Lendrem, Ann W Morgan, John D Isaacs, Arthur G Pratt
OBJECTIVES: Long-term outcomes in rheumatoid arthritis (RA) depend on early and effective disease control. Methotrexate (MTX) remains the first-line disease modifying therapy, however there are no biomarkers with which to identify those most likely to achieve remission. To address this unmet need we explored metabolic pathways involved in MTX mechanism of action within circulating CD4+T cells in a cohort of treatment naive patients with early RA. METHODS: Purified CD4+T cells were isolated from peripheral blood of 68 patients with early RA commencing MTX...
February 17, 2024: RMD Open
https://read.qxmd.com/read/38343828/assessment-of-atp-metabolism-to-adenosine-by-ecto-nucleotidases-carried-by-tumor-derived-small-extracellular-vesicles
#28
Chang-Sook Hong, Elizabeth V Menshikova, Theresa L Whiteside, Edwin K Jackson
BACKGROUND: Immunosuppression is a hallmark of cancer progression. Tumor-derived small extracellular vesicles (sEV), also known as TEX, produce adenosine (ADO) and can mediate tumor-induced immunosuppression. METHODS: Here, the ATP pathway of ADO production (ATP◊ADP◊AMP◊ADO) by ecto-nucleotidases carried in sEV was evaluated by a novel method using N6 -etheno-ATP (eATP) and N6 -etheno-AMP (eAMP) as substrates. The "downstream" N6 -etheno-purines (ePurines) were measured by high performance liquid chromatography with fluorescence detection (HPLC-FL)...
January 24, 2024: Research Square
https://read.qxmd.com/read/38340724/merkel-cell-polyomavirus-specific-and-cd39-cla-cd8-t%C3%A2-cells-as-blood-based-predictive-biomarkers-for-pd-1-blockade-in-merkel-cell-carcinoma
#29
JOURNAL ARTICLE
Heeju Ryu, Timothy M Bi, Thomas H Pulliam, Korok Sarkar, Candice D Church, Nandita Kumar, Koshlan Mayer-Blackwell, Saumya Jani, Nirasha Ramchurren, Ulla K Hansen, Sine R Hadrup, Steven P Fling, David M Koelle, Paul Nghiem, Evan W Newell
Merkel cell carcinoma is a skin cancer often driven by Merkel cell polyomavirus (MCPyV) with high rates of response to anti-PD-1 therapy despite low mutational burden. MCPyV-specific CD8 T cells are implicated in anti-PD-1-associated immune responses and provide a means to directly study tumor-specific T cell responses to treatment. Using mass cytometry and combinatorial tetramer staining, we find that baseline frequencies of blood MCPyV-specific cells correlated with response and survival. Frequencies of these cells decrease markedly during response to therapy...
January 30, 2024: Cell reports medicine
https://read.qxmd.com/read/38339054/post-transplant-cyclophosphamide-combined-with-brilliant-blue-g-reduces-graft-versus-host-disease-without-compromising-graft-versus-leukaemia-immunity-in-humanised-mice
#30
JOURNAL ARTICLE
Peter Cuthbertson, Amy Button, Chloe Sligar, Amal Elhage, Kara L Vine, Debbie Watson, Ronald Sluyter
Allogeneic haematopoietic stem cell transplantation (HSCT) leads to the establishment of graft-versus-leukaemia (GVL) immunity, but in many cases also results in the development of graft-versus-host disease (GVHD). This study aimed to determine if P2X7 antagonism using Brilliant Blue G (BBG) could improve the beneficial effects of post-transplant cyclophosphamide (PTCy) in a humanised mouse model of GVHD, without comprising GVL immunity. NOD.Cg- Prkdcscid Il2rgtm1Wjl (NSG) mice were injected with human peripheral blood mononuclear cells (PBMCs) (Day 0), then with cyclophosphamide (33 mg/kg) on Days 3 and 4, and with BBG (50 mg/kg) (or saline) on Days 0-10...
February 1, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38338057/effect-of-regular-training-on-platelet-function-in-untrained-thoroughbreds
#31
JOURNAL ARTICLE
Arianna Miglio, Emanuela Falcinelli, Katia Cappelli, Samanta Mecocci, Anna Maria Mezzasoma, Maria Teresa Antognoni, Paolo Gresele
Training has a significant effect on the physiology of blood coagulation in humans and in horses. Several hemostatic changes have been reported after exercise in the horse but data available are inconclusive. The aim of this study was to investigate platelet activation and primary platelet-related hemostasis modifications in young never-trained Thoroughbreds in the first incremental training period in order to improve knowledge on this topic. Twenty-nine clinically healthy, untrained, 2-year-old Thoroughbred racehorses were followed during their incremental 4-month sprint exercise training...
January 27, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38335302/exhausted-tumor-infiltrating-cd39-cd103-cd8-t-cells-unveil-potential-for-increased-survival-in-human-pancreatic-cancer
#32
JOURNAL ARTICLE
Laia Gorchs, Carlos Fernández-Moro, Ebba Asplund, Marlies Oosthoek, Martin Solders, Poya Ghorbani, Ernesto Sparrelid, Elena Rangelova, Matthias J Löhr, Helen Kaipe
In pancreatic ductal adenocarcinoma, the infiltration of CD8+ T cells within the tumor microenvironment correlate with a favorable prognosis. However, a significant proportion of tumor-infiltrating T cells become trapped within the desmoplastic stroma and lack tumor reactivity. Here, we explored different T-cell subsets in pancreatic tumors and adjacent tissues. We identified a subset of CD8+ T cells, double positive (DP) for CD39 and CD103 in pancreatic tumors, which has recently been described to display tumor reactivity in other types of solid tumors...
February 9, 2024: Cancer Res Commun
https://read.qxmd.com/read/38323746/genetic-insights-into-the-relationship-between-immune-cell-characteristics-and-ischemic-stroke-a-bidirectional-mendelian-randomization-study
#33
JOURNAL ARTICLE
Xia Deng, Shuai Hou, Yanqiang Wang, Haiyan Yang, Chunping Wang
BACKGROUND AND PURPOSE: Ischemic stroke, a major contributor to global disability and mortality, is underpinned by intricate pathophysiological mechanisms, notably neuroinflammation and immune cell dynamics. Prior research has identified a nuanced and often paradoxical link between immune cell phenotypes and ischemic stroke susceptibility. The aim of this study was to elucidate the potential causal links between the median fluorescence intensity (MFI) and morphological parameters (MP) of 731 immune cell types and ischemic stroke risk...
February 7, 2024: European Journal of Neurology
https://read.qxmd.com/read/38309721/efficacy-and-safety-of-autologous-tumor-infiltrating-lymphocytes-in-recurrent-or-refractory-ovarian-cancer-colorectal-cancer-and-pancreatic-ductal-adenocarcinoma
#34
JOURNAL ARTICLE
Rodabe Amaria, Anne Knisely, David Vining, Scott Kopetz, Michael J Overman, Milind Javle, Mara B Antonoff, Ching-Wei D Tzeng, Robert A Wolff, Shubham Pant, Kathryn Lito, Kelly Rangel, Bryan Fellman, Ying Yuan, Karen H Lu, Donastas Sakellariou-Thompson, Cara L Haymaker, Marie-Andrée Forget, Patrick Hwu, Chantale Bernatchez, Amir A Jazaeri
BACKGROUND: Tumor-infiltrating lymphocyte (TIL) therapy has shown efficacy in metastatic melanoma, non-small cell lung cancer, and other solid tumors. Our preclinical work demonstrated more robust CD8 predominant TIL production when agonistic anti-4-1BB and CD3 antibodies were used in early ex vivo TIL culture. METHODS: Patients with treatment-refractory metastatic colorectal (CRC), pancreatic (PDAC) and ovarian (OVCA) cancers were eligible. Lymphodepleting chemotherapy was followed by infusion of ex vivo expanded TIL, manufactured at MD Anderson Cancer Center with IL-2 and agonistic stimulation of CD3 and 4-1BB (urelumab)...
February 2, 2024: Journal for Immunotherapy of Cancer
https://read.qxmd.com/read/38306895/high-dimensional-profiling-of-regulatory-t-cells-in-psoriasis-reveals-an-impaired-skin-trafficking-property
#35
JOURNAL ARTICLE
Brian Hyohyoung Lee, Yoon Ji Bang, Sung Ha Lim, Seong-Jun Kang, Sung Hee Kim, Seunghee Kim-Schulze, Chung-Gyu Park, Hyun Je Kim, Tae-Gyun Kim
BACKGROUND: Psoriasis is a chronic inflammatory skin disease with a Th17-skewed immune phenotype. Although it has been generally accepted that regulatory T cells (Tregs) in lesional psoriatic skin have functional impairment due to the local inflammatory microenvironment, the molecular properties of skin-homing psoriatic Tregs have not been well explored. METHODS: We designed an extensive 39 marker mass cytometry (CyTOF) panel to deeply profile the immune landscape of skin-homing Tregs from 31 people with psoriasis stratified by psoriasis area severity index score as mild (n = 15) to moderate-severe (n = 16) and 32 healthy controls...
February 1, 2024: EBioMedicine
https://read.qxmd.com/read/38302493/neutral-ceramidase-regulates-breast-cancer-progression-by-metabolic-programming-of-trem2-associated-macrophages
#36
JOURNAL ARTICLE
Rui Sun, Chao Lei, Zhishan Xu, Xuemei Gu, Liu Huang, Liang Chen, Yi Tan, Min Peng, Kavitha Yaddanapudi, Leah Siskind, Maiying Kong, Robert Mitchell, Jun Yan, Zhongbin Deng
The tumor microenvironment is reprogrammed by cancer cells and participates in all stages of tumor progression. Neutral ceramidase is a key regulator of ceramide, the central intermediate in sphingolipid metabolism. The contribution of neutral ceramidase to the reprogramming of the tumor microenvironment is not well understood. Here, we find that deletion of neutral ceramidase in multiple breast cancer models in female mice accelerates tumor growth. Our result show that Ly6C+ CD39+ tumor-infiltrating CD8 T cells are enriched in the tumor microenvironment and display an exhausted phenotype...
February 1, 2024: Nature Communications
https://read.qxmd.com/read/38298016/applications-of-crispr-epigenome-editors-in-tumor-immunology-and-autoimmunity
#37
REVIEW
Berkay Yahsi, Fahreddin Palaz, Pervin Dincer
Over the past decade, CRISPR-Cas systems have become indispensable tools for genetic engineering and have been used in clinical trials for various diseases. Beyond genome editing, CRISPR-Cas systems can also be used for performing programmable epigenetic modifications. Recent efforts in enhancing CRISPR-based epigenome modifiers have yielded potent tools enabling targeted DNA methylation/demethylation capable of sustaining epigenetic memory through numerous cell divisions. Moreover, it has been understood that during chronic inflammatory states, including cancer, T cells encounter a state called T cell exhaustion that involves elevated inhibitory receptors (e...
January 31, 2024: ACS Synthetic Biology
https://read.qxmd.com/read/38296626/-establishment-of-a-21-color-panel-for-the-detection-of-immune-cell-subsets-%C3%A2-in-human-non-small-cell-lung-cancer-tumor-tissues-with-flow-cytometry
#38
JOURNAL ARTICLE
Tingting Guo, Hongguan Xie
BACKGROUND: With the rise of multicolor flow cytometry, flow cytometry has become an important means to detect the immune microenvironment of lung cancer, but most of them are used to detect the proportion of cell subsets or the function of major cell subsets, and they cannot be detected at the same time. Therefore, a reliable 21-color flow cytometry protocol was established to detect the immune cell subsets in human non-small cell lung cancer (NSCLC) tumor tissues. METHODS: Cell membrane surface antibodies cluster of differentiation (CD)45, CD3, CD19, CD4, CD8, programmed cell death 1 (PD-1), CD39, CD103, CD25, CD127, chemokine receptor 8 (CCR8), CD56, CD11c, human leukocyte antigen (HLA)-DR, CD38, CD27, CD69, CD62L, CD45RA, CCR7 and nucleic acid dye L/D were used to develop the protocol...
January 20, 2024: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://read.qxmd.com/read/38278150/inosine-induces-stemness-features-in-car-t-cells-and-enhances-potency
#39
JOURNAL ARTICLE
Dorota D Klysz, Carley Fowler, Meena Malipatlolla, Lucille Stuani, Katherine A Freitas, Yiyun Chen, Stefanie Meier, Bence Daniel, Katalin Sandor, Peng Xu, Jing Huang, Louai Labanieh, Vimal Keerthi, Amaury Leruste, Malek Bashti, Janette Mata-Alcazar, Nikolaos Gkitsas, Justin A Guerrero, Chris Fisher, Sunny Patel, Kyle Asano, Shabnum Patel, Kara L Davis, Ansuman T Satpathy, Steven A Feldman, Elena Sotillo, Crystal L Mackall
Adenosine (Ado) mediates immune suppression in the tumor microenvironment and exhausted CD8+ CAR-T cells express CD39 and CD73, which mediate proximal steps in Ado generation. Here, we sought to enhance CAR-T cell potency by knocking out CD39, CD73, or adenosine receptor 2a (A2aR) but observed only modest effects. In contrast, overexpression of Ado deaminase (ADA-OE), which metabolizes Ado to inosine (INO), induced stemness and enhanced CAR-T functionality. Similarly, CAR-T cell exposure to INO augmented function and induced features of stemness...
February 12, 2024: Cancer Cell
https://read.qxmd.com/read/38275742/altered-cd39-and-cd73-expression-in-rheumatoid-arthritis-implications-for-disease-activity-and-treatment-response
#40
JOURNAL ARTICLE
María Angels Ortiz, Cesar Diaz-Torné, Juan Jose De Agustin, Paula Estrada, Delia Reina, María Victoria Hernandez, Hye Sang, Carlos Zamora, Elisabet Cantó, Hector Corominas, Silvia Vidal
In rheumatoid arthritis (RA) synovium, ATP, and ADP are released, sparking inflammation. Ectoenzymes CD39 and CD73 metabolize these purine nucleotides, generating anti-inflammatory adenosine. Therefore, dysregulated CD39 and CD73 expression may impact RA development. We assessed CD39 and CD73 expression in peripheral blood from 15 healthy controls (Cs) and 35 RA patients at baseline and after 3 and 6 months of tocilizumab treatment using flow cytometry. Additionally, ectoenzyme expression was examined on cultured T cells to understand activation and IL-6 effects...
December 19, 2023: Biomolecules
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