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https://www.readbyqxmd.com/read/29236364/intraflagellar-transporter-protein-140-ift140-a-component-of-ift-a-complex-is-essential-for-male-fertility-and-spermiogenesis-in-mice
#1
Yong Zhang, Hong Liu, Wei Li, Zhengang Zhang, Shiyang Zhang, Maria E Teves, Courtney Stevens, James A Foster, Gregory E Campbell, Jolene J Windle, Rex A Hess, Gregory J Pazour, Zhibing Zhang
Intraflagellar transport (IFT) is a conserved mechanism essential for the assembly and maintenance of most eukaryotic cilia and flagella. However, little is known about its role in sperm flagella formation and male fertility. IFT140 is a component of IFT-A complex. In mouse, it is highly expressed in the testis. Ift140 gene was inactivated specifically in mouse spermatocytes/spermatids. The mutant mice did not show any gross abnormalities, but all were infertile associated with significantly reduced sperm number and motility...
December 13, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/29236349/direct-and-enantioselective-synthesis-of-n-h-free-1-5-benzodiazepin-2-ones-via-nhc-catalyzed-3-4-annulation-reaction
#2
Ding Du, Jing Cao, Chao Fang, Kewen Sun, Jindong Zhu, Tao Lu
An NHC-catalyzed formal [3+4] annulation of alfa,beta-unsaturated acylazoliums with protecting group-free aryl 1,2-diamines was developed for a direct and highly enantioselective synthesis of 4-aryl N-H-free 1,5-benzodiazepin-2-ones. This methodology offers an efficient and rapid access to a wide range of enantioenriched target compounds from easily accessible starting materials. The protocol is also scalable and the desired products can easily undergo subsequent N-functionalization to afford diverse N-substituted derivatives...
December 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29236333/effect-and-mechanism-of-lncrna-hotair-on-occurrence-and-development-of-gastric-cancer
#3
Xuan Feng, Suiping Huang
OBJECTIVE: In this study, we aimed at looking for the lncRNA abnormally expressed in gastric cancer (GC) tissues, and analyzing the association of its expression level with the clinicopathological feature and patient prognosis. The biological function of this lncRNA in occurrence and development of GC was studied in vivo and in vitro. The underlying molecular mechanism was further discussed. METHODS: We investigated the lncRNA expression level in GC tissues and normal gastric mucosa tissues by lncRNA chips analysis...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236332/a-new-mechanism-for-%C3%AE-lactamases-class-d-enzymes-degrade-1%C3%AE-methyl-carbapenems-via-lactone-formation
#4
Christopher T Lohans, Emma van Groesen, Kiran Kumar, Catherine L Tooke, James Spencer, Robert S Paton, Jürgen Brem, Christopher Schofield
β-Lactamases threaten the clinical use of carbapenems, considered antibiotics of last resort. The classical mechanism of serine carbapenemase activity proceeds via hydrolysis of an acyl-enzyme intermediate. We show that class D β-lactamases also degrade clinically used 1β-methyl-substituted carbapenems via the unprecedented formation of a carbapenem-derived β-lactone. β-Lactone formation results from the nucleophilic attack of the carbapenem hydroxyethyl side chain onto the ester carbonyl of the acyl-enzyme intermediate...
December 13, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29236326/knockdown-of-ski-decreased-the-reactive-astrocytes-proliferation-in-vitro-induced-by-oxygen-glucose-deprivation-reoxygenation
#5
Xin Zhao, Kai-Sheng Zhou, Zhong-Hao Li, Wei Nan, Jing Wang, Ya-Yi Xia, Hai-Hong Zhang
Glia scar is a pathological marker in late phase of brain ischemia disease, which constitutes a major physical biochemical barrier to impede axonal regrowth. Astrocytes are known to be critically involved in the formation of glial scar. However, their response to ischemia and their role in neuroprotection after central nervous system (CNS) injury are not completely clear. Recently, we have demonstrated for the first time that Ski was up-regulated in reactive astrocytes after spinal cord injury in vivo and in vitro, which indicates Ski may be a new molecule that control astrocytes biologic properties after CNS injury...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236325/aml1-eto-trans-activates-c-kit-expression-through-the-long-range-interaction-between-promoter-and-intronic-enhancer
#6
Ying Tian, Genjie Wang, Qingzhu Hu, Xichun Xiao, Shuxia Chen
The AML1/ETO onco-fusion protein is crucial for the genesis of t(8;21) acute myeloid leukemia (AML) and is well documented as a transcriptional repressor through dominant-negative effect. However, little is known about the transactivation mechanism of AML1/ETO. Through large cohort of patient's expression level data analysis and a series of experimental validation, we report here that AML1/ETO transactivates c-KIT expression through directly binding to and mediating the long-range interaction between the promoter and intronic enhancer regions of c-KIT...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236324/silencing-of-phospholipase-c-gamma-2-promotes-proliferation-of-rat-hepatocytes-in-vitro
#7
Xiaoguang Chen, Xuemin Zhu, Yumei Liu, Qiongxia Lv, Jun Ma
The management of hepatic failure is undoubtedly difficult, and poor results have led to the search for novel therapeutic approaches. Nowadays, anti-apoptotic gene therapy is considered as an ideal approach. It has been proved that phospholipase Cγ2 (PLCγ2) is involved in the apoptosis of immune cells and tumor cells; however, whether this gene is related to hepatocyte death is still unclear. This study examined the role of PLCγ2 by inhibiting its expression in rat hepatocytes with siRNA. We also further analyzed the cellular mechanism by which the expression inhibition of PLCγ2 induces cell death...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236320/analgesic-effects-of-microrna-129-5p-against-bone-cancer-pain-through-the-ephb1-ephrinb2-signaling-pathway-in-mice
#8
Shao-Nan Yu, Gui-Feng Liu, Long-Yun Li, Guo-Qing Zhao, Lin Liu, Xue-Feng Li
The study aims to investigate the analgesic effects of microRNA-129-5p (miR-129-5p) on bone cancer pain (BCP) by targeting Eph receptor B1 (EphB1) through the EphB1/EphrinB2 signaling pathway. BCP mice models were established, and C3H/HeJ female mice were classified into the normal, blank, negative control (NC), miR-129-5p mimics, miR-129-5p inhibitors, EphB1 knockout (KO), and miR-129-5p inhibitors + EphB1 KO groups. RT-qPCR and western blotting were used to evaluate miR-129-5p expression, and mRNA and protein expressions of EphB1, p-EphB1, EphrinB2 and p-EphrinB2...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236316/2-5-hexanedione-induces-bone-marrow-mesenchymal-stem-cell-apoptosis-via-inhibition-of-akt-bad-signal-pathway
#9
Jinsong Sun, Xiaoxia Shi, Shuangyue Li, Fengyuan Piao
2,5-Hexanedione (HD) is an important bioactive metabolite of n-hexane and mediates the neurotoxicity of parent compound. Studies show that HD induces apoptotic death of neural progenitor cells. However, its underlying mechanism remains unknown. Mesenchymal stem cells (MSCs) are multipotential stem cells with the ability to differentiate into various cell types and have been used as cell model for studying the toxic effects of chemicals on stem cells. In this study, we exposed rat bone marrow MSCs to 0, 10, 20, and 40 mM HD in vitro...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236315/lnc-rna-snhg20-participated-in-proliferation-invasion-and-migration-of-breast-cancer-cells-via-mir-495
#10
Yan-Xing Guan, Zhan-Min Zhang, Xue-Zhong Chen, Qing Zhang, Shao-Zheng Liu, Yong-Lu Zhang
OBJECTIVE: To explore the mechanism of lnc SNHG20 in the regulation of proliferation, invasion and migration of breast cancer cells. MATERIALS AND METHODS: mRNA levels of SNHG20, miR-495 and HER2 were detected by qRT-PCR. Protein level of HER2 was measured by Western blot. Cell proliferation, invasion and migration were detected by CCK-8 assay, Boyden chamber assay and Transwell assay. The combination between SNHG20 and miR-495 was confirmed by RNA pull down assay...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236311/mir-221-negatively-regulates-inflammation-and-insulin-sensitivity-in-white-adipose-tissue-by-repression-of-sirtuin-1-sirt1
#11
Jie Peng, Yuanfei Zhou, Zhao Deng, Hong Zhang, Yinghui Wu, Tongxing Song, Yang Yang, Hongkui Wei, Jian Peng
It is well known that obesity-induced white adipose tissue inflammation is an important reason for insulin-resistance and type 2 diabetes mellitus. Sirtuin-1 (SIRT1) is an important regulator of inflammtion response pathways in white adipose tissue. Here, we found that miR-221 negatively regulated SIRT1 in white adipose tissue during inflammation and HFD-induced obesity. MiR-221 is a putative oncogene which has been found overexpressed in a number of human tumors. Recently, it has also found that miR-221 was increased in obese adipose tissue and may be involved in inflammation and insulin-resistance...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236309/leptin-induces-mmp1-13-and-adamts-4-expressions-through-bone-morphogenetic-protein-2-autocrine-effect-in-human-chondrocytes
#12
Yu-Ping Su, Cheng-Nan Chen, Kuo-Chin Huang, Hsin-I Chang, Ko-Chao Lee, Chun-Min Lo, Shun-Fu Chang
The induction of bone morphogenetic protein (BMP) 2 in injured and arthritis articular cartilage has been proposed, but the precise mechanism has not been clearly clarified. Our previous study has found that leptin could stimulate the BMP2 autocrine effect to increase the anabolic collagen II expression when it initiates the catabolic response in human chondrocytes. It has been suggested that this BMP2 autocrine effect contributes to a reparative role in leptin-stimulated human chondrocytes. In this study, we further determined whether this BMP2 autocrine effect also affect the expressions of catabolic matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236308/database-of-transcription-factors-in-lung-cancer-dbtflc-a-novel-resource-for-exploring-transcription-factors-associated-with-lung-cancer
#13
A Thabitha, Ameya Athul Dravid, Riya Tripathi, S Sajitha Lulu
Lung cancer is considered as the most prevalent form of cancer and it is found to be frequent cause of cancer related death. Even though, approved molecular targeted therapies other than chemotherapy are currently unavailable, the mechanism of pathogenesis in lung cancer remains still unclear. Transcription factors (TFs) play a critical role in cancer cell processes, such as cell proliferation, apoptosis, migration, and regulate gene expression. Thus, the identification and characterization of transcription factors involved in lung cancer would provide valuable information for further elucidation of the mechanism(s) underlying pathogenesis and the identification of potential therapeutic target types, which are critical for the development of therapeutic strategies...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236306/rs4705342-polymorphism-is-involved-in-the-tumorigenesis-of-hbv-positive-hcc-by-altering-the-binding-affinity-of-hbv-induced-nf-kb-with-the-promoter-region-of-microrna-143
#14
Yin Xiuli, Sun Shiying, Zhao Junyan, Yang Jing, Lei Xiaofei, Xu Changqing, Li Kun
BACKGROUND: The objective of this study was to explore the role of rs4705342 located in the miR-143 promoter in relation to the control of HBV positive HCC and the underlying molecular mechanism. METHOD: A luciferase assay was performed to explore the factors which influenced miR-143 transcription activity and the target gene of miR-143. This would further be confirmed by ChIP assay. Western-blot and real-time PCR were performed to identify the relationship between miR-143 and ORP8...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236261/reactivation-assay-to-identify-direct-targets-of-the-nonsense-mediated-mrna-decay-pathway-in-drosophila
#15
Jonathan O Nelson, Mark M Metzstein
Transcriptome analysis provides a snapshot of cellular gene expression and is used to determine how cells and organisms respond to genetic or environmental changes. Identifying the transcripts whose expression levels are regulated directly by the manipulation being examined from those whose expression changes as a secondary cause from the primary changes requires additional analyses. Here we present a technique used to distinguish direct targets of the nonsense-mediated mRNA decay (NMD) pathway in Drosophila from secondary gene expression effects caused by loss of this pathway...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236260/generation-of-cell-lines-stably-expressing-a-fluorescent-reporter-of-nonsense-mediated-mrna-decay-activity
#16
Nadezhda M Markina, Anton P Pereverzev, Dmitry B Staroverov, Konstantin A Lukyanov, Nadya G Gurskaya
Nonsense-mediated mRNA decay (NMD) is a mechanism of mRNA surveillance ubiquitous among eukaryotes. Importantly, NMD not only removes aberrant transcripts with premature stop codons, but also regulates expression of many normal genes. A recently introduced dual-color fluorescent protein-based reporter enables analysis of NMD activity in live cells. In this chapter we describe the method to generate stable transgenic cell lines expressing the splicing-dependent NMD reporter using consecutive steps of lentivirus transduction and Tol2 transposition...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236250/measuring-mrna-decay-in-budding-yeast-using-single-molecule-fish
#17
Tatjana Trcek, Samir Rahman, Daniel Zenklusen
Cellular mRNA levels are determined by the rates of mRNA synthesis and mRNA decay. Typically, mRNA degradation kinetics are measured on a population of cells that are either chemically treated or genetically engineered to inhibit transcription. However, these manipulations can affect the mRNA decay process itself by inhibiting regulatory mechanisms that govern mRNA degradation, especially if they occur on short time-scales. Recently, single molecule fluorescent in situ hybridization (smFISH) approaches have been implemented to quantify mRNA decay rates in single, unperturbed cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236234/assessment-of-the-functional-impact-of-germline-brca1-2-variants-located-in-non-coding-regions-in-families-with-breast-and-or-ovarian-cancer-predisposition
#18
E Santana Dos Santos, S M Caputo, L Castera, M Gendrot, A Briaux, M Breault, S Krieger, P K Rogan, E J Mucaki, L J Burke, I Bièche, C Houdayer, D Vaur, D Stoppa-Lyonnet, M A Brown, F Lallemand, E Rouleau
PURPOSE: The molecular mechanism of breast and/or ovarian cancer susceptibility remains unclear in the majority of patients. While germline mutations in the regulatory non-coding regions of BRCA1 and BRCA2 genes have been described, screening has generally been limited to coding regions. The aim of this study was to evaluate the contribution of BRCA1/2 non-coding variants. METHODS: Four BRCA1/2 non-coding regions were screened using high-resolution melting analysis/Sanger sequencing or next-generation sequencing on DNA extracted from index cases with breast and ovarian cancer predisposition (3926 for BRCA1 and 3910 for BRCA2)...
December 13, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29236227/loss-of-vascular-expression-of-nucleoside-triphosphate-diphosphohydrolase-1-cd39-in-hypertension
#19
Charlotte Roy, Julie Tabiasco, Antoine Caillon, Yves Delneste, Jean Merot, Julie Favre, Anne Laure Guihot, Ludovic Martin, Daniele C Nascimento, Bernhard Ryffel, Simon C Robson, Jean Sévigny, Daniel Henrion, Gilles Kauffenstein
Ectonucleoside triphosphate diphosphohydrolase-1, the major vascular/immune ectonucleotidase, exerts anti-thrombotic and immunomodulatory actions by hydrolyzing extracellular nucleotides (danger signals). Hypertension is characterized by vascular wall remodeling, endothelial dysfunction, and immune infiltration. Here our aim was to investigate the impact of arterial hypertension on CD39 expression and activity in mice. Arterial expression of CD39 was determined by reverse transcription quantitative real-time PCR in experimental models of hypertension, including angiotensin II (AngII)-treated mice (1 mg/kg/day, 21 days), deoxycorticosterone acetate-salt mice (1% salt and uninephrectomy, 21 days), and spontaneously hypertensive rats...
December 13, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29236219/radioresistant-sf9-insect-cells-readily-undergo-an-intrinsic-mode-of-apoptosis-in-response-to-histone-deacetylase-hdac-inhibition
#20
Jyoti Swaroop Kumar, Shubhankar Suman, Sudhir Chandna
Insect cell lines have been utilized as an important higher eukaryotic model system to decipher stress responses and cell death mechanisms. Lepidopteran Sf9 cells (derived from the ovaries of Spodoptera frugiperda) display nearly 100 times higher resistance to ionizing radiation in contrast to mammalian cells, which is partly contributed by an unusually high HDAC activity. However, their response to HDAC inhibition remains to be evaluated. In the present study, the effects of HDAC inhibitor (NaBt) on Sf9 cellular/nuclear morphology, cell cycle progression, DNA damage/repair, redox status, and mitochondrial perturbations were evaluated...
December 13, 2017: Molecular and Cellular Biochemistry
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