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https://www.readbyqxmd.com/read/28527284/-new-multiplex-pcr-for-species-specific-diagnosis-of-human-candidiasis
#1
Liliana Torcoroma García, Liany Johanna Luna, Tania Katherine Velasco, Beatriz Elena Guerra
INTRODUCTION: Candidiases is a group of opportunistic infections caused by yeasts belonging to the genus Candida. Candida albicans is the most prevalent species in both superficial and deep infections, however, the clinical importance of non-albicans Candida has increased during the last decade, driving an urgent need for diagnostic tests that allow for species-level resolution and selection of the optimum therapeutic approach. OBJECTIVE: To design and to optimize a new multiplex PCR assay for the simultaneous identification of the five most relevant species of Candida involved in human candidiasis etiology...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527158/asymptomatic-cryptococcal-antigenemia-in-people-living-with-hiv-plhiv-with-severe-immunosuppression-is-routine-crag-screening-indicated-in-india
#2
S Anuradha, Abhaya Narayana H, Richa Dewan, Ravinder Kaur, K Rajeshwari
BACKGROUND: Cryptococcal meningitis (CM) is a common, life-threatening opportunistic infection (OI) among people living with HIV (PLHIV) in India. Serum Cryptococcal antigen (CrAg) positivity is predictive of future occurrence of CM and pre-emptive treatment reduces its mortality. Routine CrAg screening among PLHIV is not adopted by India's national programme. This study evaluated the prevalence of CrAg and assessed CrAg positivity in predicting all-cause mortality among PLHIV. METHODS: This prospective study was conducted in a tertiary care, public health facility in New Delhi, India...
April 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28523422/usefulness-of-the-non-conventional-caenorhabditis-elegans-model-to-assess-candida-virulence
#3
Marcelo Ortega-Riveros, Iker De-la-Pinta, Cristina Marcos-Arias, Guillermo Ezpeleta, Guillermo Quindós, Elena Eraso
Invasive candidiasis is caused mainly by Candida albicans, but other Candida species have increasing etiologies. These species show different virulence and susceptibility levels to antifungal drugs. The aims of this study were to evaluate the usefulness of the non-conventional model Caenorhabditis elegans to assess the in vivo virulence of seven different Candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and biofilm development capacity...
May 18, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28521529/potential-of-novel-drug-delivery-systems-in-the-management-of-topical-candidiasis
#4
Mahima Mathur, Kusum Devi Vemula
High prevalence of topical fungal infections is perceived in majority of nations worldwide accounting for numerous serious systemic complications. Of several fungal infections, candidiasis is one of the widespread infections which is manifested due to localization and proliferation of fungi. Present pharmacotherapy offers an effective treatment but possesses serious limitations like inadequate solubility, ineffectiveness in lowering diseased condition and patient incompliance. Several attempts to overcome these shortcomings and building suitable technology platforms for development of appropriate dosage forms which can enhance effectiveness, patient acceptability while maintaining safety, efficacy and affordability of drug delivery, have been made...
May 18, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28516061/the-effect-of-vaginal-cream-containing-ginger-in-users-of-clotrimazole-vaginal-cream-on-vaginal-candidiasis
#5
Sheida Shabanian, Sima Khalili, Zahra Lorigooini, Afsaneh Malekpour, Saeid Heidari-Soureshjani
Vulvovaginal candidiasis is one of the most common infections of the genital tract in women that causes many complications. Therefore, we examined the clinical effect of ginger cream along with clotrimazole compared to vaginal clotrimazole alone in this study. This double-blind clinical trial was conducted on 67 women admitted to the Gynecology Clinic of Hajar Hospital with vaginal candidiasis. The patients were divided randomly into two groups of 33 and 34 people. The diagnosis was made according to clinical symptoms, wet smear, and culture...
April 2017: Journal of Advanced Pharmaceutical Technology & Research
https://www.readbyqxmd.com/read/28515716/antifungal-activity-of-the-biphosphinic-cyclopalladate-c7a-against-candida-albicans-yeast-forms-in-vitro-and-in-vivo
#6
Julian E Muñoz, Diego C P Rossi, Kelly Ishida, Cristina C Spadari, Marcia S C Melhem, Daniel M Garcia, Antonio C F Caires, Carlos P Taborda, Elaine G Rodrigues
Vulvovaginal and invasive candidiasis are frequent conditions in immunosuppressed individuals caused by Candida albicans and non-albicans Candida spp. Fluconazole and Amphotericin B are the main drugs used to fight the infection. However, resistance to fluconazole and other azole antifungal drugs is an important clinical problem that encourages the search for new therapeutic alternatives. In this work, we evaluate the antifungal activity of the biphosphinic cyclopalladate C7a in the in vitro and in vivo model...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28511604/duplicated-or-hybridized-peptide-functional-domains-promote-oral-homeostasis
#7
T Basiri, N D Johnson, E B Moffa, Y Mulyar, P L Serra Nunes, M A A M Machado, W L Siqueira
Proteins that have existed for millions of years frequently contain repeats of functional domains within their primary structure, thereby improving their functional capacity. In the evolutionary young statherin protein contained within the in vivo-acquired enamel pellicle (AEP), we identified a single functional domain (DR9) located within the protein N-terminal portion that exhibits a higher affinity for hydroxyapatite and more efficient protection against enamel demineralization compared to other native statherin peptides...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28511380/fungal-profile-of-vulvovaginal-candidiasis-in-a-tertiary-care-hospital
#8
Krishnapriya Kalaiarasan, Rakesh Singh, Latha Chaturvedula
INTRODUCTION: Vulvovaginal Candidiasis (VVC) is a common medical health problem of adult women. It is most commonly caused by Candida albicans. But there is a change in fungal profile. Sabouraud's Dextrose Agar (SDA) is the most common culture medium used where mixed fungal infection may be missed. It can be detected easily by using chromogenic culture medium. AIM: To know the fungal profile of vulvovaginal candidiasis using Candida CHROMagar and antifungal susceptibility pattern in patients attending tertiary care hospital...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28509514/lay-public-s-use-of-a-support-group-for-general-dental-problems
#9
K K Walker, R D Jackson
OBJECTIVE: To categorize and tabulate layperson inquiries made to an Internet dental health support site to identify oral conditions and associated behaviors of concern. METHODS: A retrospective tabulation of wall postings was made from an established dental health support website hosted by WebMD over a 2-year period (April 2013-April 2015). A mixed method approach of content and thematic analysis was used. Content analysis identified content of oral health concerns, while thematic analysis using grounded theory identified themes and beliefs concerning associated behaviors...
September 2016: Community Dental Health
https://www.readbyqxmd.com/read/28507352/candidiasis-prevalence-and-resistance-profiling-in-a-tertiary-care-hospital-of-pakistan
#10
Bushra Jamil, Mohammad Tauseef Mukhtar Bokhari, Azhar Saeed, Mohammad Zahid Mukhtar Bokhari, Zakir Hussain, Tayyaba Khalid, Habib Bokhari, Mohammad Imran, Shahid Ahmad Abbasi
OBJECTIVE: To determine Candida colonisation/infection in renal transplant patients and to determine the resistance pattern against antifungal drugs. METHODS: This prospective, observational study was conducted at Al-Sayyed Hospital, Rawalpindi, Pakistan, from January to October 2014, in collaboration with the Microbiology and Public Health Laboratory's, Islamabad campus..The clinical specimens investigated included respiratory tract secretions, blood, urine, high vaginal swab, skin scrapings, and plastic devices samples...
May 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28507109/erg11-gene-mutations-and-mdr1-up-regulation-confer-pan-azole-resistance-in-candida-tropicalis-causing-disseminated-candidiasis-while-on-posaconazole-prophylaxis-in-an-acute-lymphoblastic-leukemia-patient
#11
Liangshun You, Wenbin Qian, Qing Yang, Liping Mao, Li Zhu, Xianbo Huang, Jie Jin, Haitao Meng
In this study, we presented a rare case of fatal breakthrough Candida tropicalis in a patient with acute lymphoblastic leukemia (ALL) while on posaconazole prophylaxis. And then we explored the mechanisms underlying azole resistance by focusing on enhanced efflux pumps and changes in the azole target enzyme Erg11p, which was encoded by the ERG11 gene. Our study demonstrates that Y132C substitution of Erg11p combined with MDR1 overexpression may be the pan-azole-resistance mechanisms in Candida tropicalis.
May 15, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28506303/erratum-to-contribution-of-candida-biomarkers-and-dna-detection-for-the-diagnosis-of-invasive-candidiasis-in-icu-patients-with-severe-abdominal-conditions
#12
Cristóbal León, Sergio Ruiz-Santana, Pedro Saavedra, Carmen Castro, Ana Loza, Ismail Zakariya, Alejandro Úbeda, Manuel Parra, Desirée Macías, José Ignacio Tomás, Antonio Rezusta, Alejandro Rodríguez, Frederic Gómez, Estrella Martín-Mazuelos
No abstract text is available yet for this article.
May 15, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/28502874/aerobic-vaginitis-no-longer-a-stranger
#13
Gilbert G G Donders, Gert Bellen, Svitrigaile Grinceviciene, Kateryna Ruban, Pedro Vieira-Baptista
Aerobic vaginitis (AV) is the name given in 2002 to a vaginal infectious entity which was not recognized as such before. It is characterized by abnormal (dysbiotic) vaginal microflora containing aerobic, enteric bacteria, variable levels of vaginal inflammation and deficient epithelial maturation. Although AV and bacterial vaginosis (BV) share some characteristics, such as a diminished number or absence of lactobacilli, increased discharge (fishy smelling in BV, while in severe forms of AV, a foul, rather rotten smell may be present) and increased pH (often more pronounced in AV), there are also striking differences between the two...
May 11, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28501934/cx-4945-the-protein-kinase-ck2-inhibitor-and-anti-cancer-drug-shows-anti-fungal-activity
#14
Maciej Masłyk, Monika Janeczko, Aleksandra Martyna, Konrad Kubiński
CX-4945 is a selective inhibitor of protein kinase CK2 exhibiting clinical significance. Its antitumor properties arise from the abrogation of CK2-mediated pro-survival cellular pathways. The presented data reveal the influence of CX-4945 on the growth of yeast cells showing variable potency against Saccharomyces cerevisiae deletion strains with different contents of CK2 subunits. The catalytic subunit CK2α appears to sensitize yeast to the CX-4945 action. Moreover, the compound suppresses hyphal growth and cell adhesion of Candida albicans, thereby abolishing some hallmarks of invasiveness of the pathogen...
May 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28501465/candiduria-evidence-based-approach-to-management-are-we-there-yet
#15
REVIEW
W A Alfouzan, R Dhar
Candiduria is considered one of the most controversial issues in patient management. Neither the diagnosis nor the optimal treatment options are standardized. This is further complicated by lack of defined laboratory criteria for diagnosis as most of the studies were set for bacterial rather than fungal urinary tract infection (UTI). Furthermore, since Candida species is a known commensal of the genitourinary tract its presence in the urine sample adds ambiguity to making a definitive diagnosis of candidal UTI...
May 10, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28500570/narrow-band-imaging-can-increase-the-visibility-of-fibrin-caps-after-bleeding-of-esophageal-varices-a-case-with-extensive-esophageal-candidiasis
#16
Yoshihiro Furuichi, Yoshitaka Kasai, Hirohito Takeuchi, Yuu Yoshimasu, Takashi Kawai, Katsutoshi Sugimoto, Yoshiyuki Kobayashi, Ikuo Nakamura, Takao Itoi
A 58-year-old man with hepatitis B cirrhosis noticed black stools and underwent an endoscopy at a community hospital. The presence of esophageal varices (EVs) was confirmed, but the bleeding point was not found. He was referred to our institution and underwent a second endoscopy. Extensive white patches of esophageal candidiasis were visible on endoscopy by white-light imaging (WLI), but it was difficult to find the fibrin cap of the EVs. This was easier under narrow-band imaging (NBI), however, as the color turned red from absorption by hemoglobin adhered to it...
May 12, 2017: Clinical Journal of Gastroenterology
https://www.readbyqxmd.com/read/28499959/impact-of-initial-empirical-antifungal-agents-on-the-outcome-of-critically-ill-patients-with-invasive-candidiasis-analysis-of-the-china-scan-study
#17
Na Cui, Hao Wang, Haibo Qiu, Ruoyu Li, Dawei Liu
The effect of different empirical antifungal agents on the clinical outcome of critically ill patients with invasive candidiasis (IC) has not been fully elucidated. In this study, 136 patients with proven IC who received empirical therapy in the China-SCAN multicentre study were retrospectively analysed. Initial empirical antifungal monotherapy consisted of a triazole [fluconazole (n = 61), voriconazole (n = 20) or itraconazole (n = 12)] or an echinocandin (n = 43). Hospital mortality as the primary outcome and global responses (clinical and microbiological) were assessed...
May 9, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28499528/vaginitis-beyond-the-basics
#18
REVIEW
Benjie Brown Mills
Vaginal complaints are one of the most common reasons women seek the advice of a health care provider. Uncomplicated infections such as vulvovaginal candidiasis, bacterial vaginosis, or trichomoniasis are easy to diagnose and treat. However, about 8% of patients will have a more complicated course with failure to respond to treatment or rapid recurrence of symptoms. Understanding the need for a methodical, diagnostic approach to help these women with recurrent or refractory cases of vaginal symptoms will aid the clinician achieve successful patient outcomes...
June 2017: Obstetrics and Gynecology Clinics of North America
https://www.readbyqxmd.com/read/28494177/influence-of-low-level-laser-on-pain-and-inflammation-in-type-2-diabetes-mellitus-with-diabetic-dermopathy-a-case-report
#19
Animesh Hazari, Shivashankara K N, Karthik K Rao, Arun G Maiya
Numerous skin lesions have been commonly observed in individuals with diabetes mellitus. The common skin manifestations of diabetes mellitus are erythrsma, xanthosmatosis, xanthelasma, pycomycetes, and cutaneous infections like furuncolosis, candidiasis, carbuncle, dermatophytosis etc.Diabetic dermopathy is the most common skin lesion found in diabetics. It is typically seen in males above 50 years. Low level laser therapy was illuminated on the entire lower limb with the frequency of 20 Hz and wavelength of 830 nm for 9 minutes divided into four parts...
May 11, 2017: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/28492295/elderly-versus-non-elderly-patients-with-intra-abdominal-candidiasis-in-the-icu
#20
George Dimopoulos, Dimitrios K Matthaiou, Elda Righi, Maria Merelli, Matteo Bassetti
BACKGROUND: Intra-abdominal candidiasis (IAC) has a considerable cost in terms of mortality and morbidity. We sought to study the epidemiology, characteristics and outcome of elderly (>75 years old) versus non-elderly patients with IAC and risk factors for mortality in elderly patients. METHODS: Post-hoc analysis of a retrospective multinational cohort study over a 3- year period (2011-2013). RESULTS: Of 482 patients, 124 (25.7%) were elderly and 358 (74...
May 11, 2017: Minerva Anestesiologica
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