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Two photon microscopy

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https://www.readbyqxmd.com/read/28345902/hyaluronate-peanut-agglutinin-conjugates-for-target-specific-bioimaging-of-colon-cancer
#1
Songeun Beack, Minsoo Cho, Young-Eun Kim, G-One Ahn, Sei Kwang Hahn
Colon cancer is one of the most common death-related cancers in the world. For treating the colon cancer, it is crucial to detect and remove malignant lesions in the early time. Here, we developed hyaluronate (HA) - peanut agglutinin (PNA) conjugates for bioimaging of colon cancer. The HA-PNA conjugates were successfully synthesized by the coupling reaction between aldehyde modified HA and the N-terminal amine group of PNA. For diagnostic imaging, rhodamine B (RhoB) was chemically conjugated onto PNA in HA-PNA conjugates...
March 27, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28343256/spatiotemporal-deep-imaging-of-syncytium-induced-by-the-soybean-cyst-nematode-heterodera-glycines
#2
Mina Ohtsu, Yoshikatsu Sato, Daisuke Kurihara, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama, Tetsuya Higashiyama
Parasite infections cause dramatic anatomical and ultrastructural changes in host plants. Cyst nematodes are parasites that invade host roots and induce a specific feeding structure called a syncytium. A syncytium is a large multinucleate cell formed by cell wall dissolution-mediated cell fusion. The soybean cyst nematode (SCN), Heterodera glycines, is a major soybean pathogen. To investigate SCN infection and the syncytium structure, we established an in planta deep imaging system using a clearing solution ClearSee and two-photon excitation microscopy (2PEM)...
March 25, 2017: Protoplasma
https://www.readbyqxmd.com/read/28335325/nanoscale-electric-characteristics-and-oriented-assembly-of-halobacterium-salinarum-membrane-revealed-by-electric-force-microscopy
#3
Denghua Li, Yibing Wang, Huiwen Du, Shiwei Xu, Zhemin Li, Yanlian Yang, Chen Wang
Purple membranes (PM) of the bacteria Halobacterium salinarum are a unique natural membrane where bacteriorhodopsin (BR) can convert photon energy and pump protons. Elucidating the electronic properties of biomembranes is critical for revealing biological mechanisms and developing new devices. We report here the electric properties of PMs studied by using multi-functional electric force microscopy (EFM) at the nanoscale. The topography, surface potential, and dielectric capacity of PMs were imaged and quantitatively measured in parallel...
November 2, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28328541/molecular-engineering-of-two-photon-fluorescent-probes-for-bioimaging-applications
#4
Hong-Wen Liu, Yongchao Liu, Peng Wang, Xiao-Bing Zhang
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28324601/two-photon-intravital-microscopy-animal-preparation-protocol-to-study-cellular-dynamics-in-pathogenesis
#5
Erinke van Grinsven, Chloé Prunier, Nienke Vrisekoop, Laila Ritsma
Two-photon intravital microscopy (2P-IVM) is an advanced imaging platform that allows the visualization of dynamic processes at subcellular resolution in vivo. Dynamic processes like cell migration, cell proliferation, cell-cell interactions, and cell signaling have an interactive character and occur in complex environments. Hence, it is of pivotal importance to study these processes in living animals, using for example 2P-IVM. 2P-IVM can be performed on a variety of tissues, from the skin of the animal to internal organs, and a variety of methods can be utilized to perform 2P-IVM on these tissues...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28323283/treadmill-exercise-suppressed-stress-induced-dendritic-spine-elimination-in-mouse-barrel-cortex-and-improved-working-memory-via-bdnf-trkb-pathway
#6
K Chen, L Zhang, M Tan, C S W Lai, A Li, C Ren, K-F So
Stress-related memory deficit is correlated with dendritic spine loss. Physical exercise improves memory function and promotes spinogenesis. However, no studies have been performed to directly observe exercise-related effects on spine dynamics, in association with memory function. This study utilized transcranial two-photon in vivo microscopy to investigate dendritic spine formation and elimination in barrel cortex of mice under physical constrain or naive conditions, followed by memory performance in a whisker-dependent novel texture discrimination task...
March 21, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28319608/cerebellar-granule-cells-acquire-a-widespread-predictive-feedback-signal-during-motor-learning
#7
Andrea Giovannucci, Aleksandra Badura, Ben Deverett, Farzaneh Najafi, Talmo D Pereira, Zhenyu Gao, Ilker Ozden, Alexander D Kloth, Eftychios Pnevmatikakis, Liam Paninski, Chris I De Zeeuw, Javier F Medina, Samuel S-H Wang
Cerebellar granule cells, which constitute half the brain's neurons, supply Purkinje cells with contextual information necessary for motor learning, but how they encode this information is unknown. Here we show, using two-photon microscopy to track neural activity over multiple days of cerebellum-dependent eyeblink conditioning in mice, that granule cell populations acquire a dense representation of the anticipatory eyelid movement. Initially, granule cells responded to neutral visual and somatosensory stimuli as well as periorbital airpuffs used for training...
March 20, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28319259/label-free-identification-of-the-microstructure-of-rat-spinal-cords-based-on-nonlinear-optical-microscopy
#8
C X Liao, Z Y Wang, Y Zhou, L Q Zhou, X Q Zhu, W G Liu, J X Chen
The spinal cord is a vital link between the brain and the body and mainly comprises neurons, glial cells and nerve fibres. In this work, nonlinear optical (NLO) microscopy based on intrinsic tissue properties was employed to label-freely analyze the cells and matrix in spinal cords at a molecular level. The high-resolution and high-contrast NLO images of unstained spinal cords demonstrate that NLO microscopy has the ability to show the microstructure of white and grey matter including ventral horn, intermediate area, dorsal horns, ventral column, lateral column and dorsal column...
March 20, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28319111/volumetric-two-photon-imaging-of-neurons-using-stereoscopy-vtwins
#9
Alexander Song, Adam S Charles, Sue Ann Koay, Jeff L Gauthier, Stephan Y Thiberge, Jonathan W Pillow, David W Tank
Two-photon laser scanning microscopy of calcium dynamics using fluorescent indicators is a widely used imaging method for large-scale recording of neural activity in vivo. Here, we introduce volumetric two-photon imaging of neurons using stereoscopy (vTwINS), a volumetric calcium imaging method that uses an elongated, V-shaped point spread function to image a 3D brain volume. Single neurons project to spatially displaced 'image pairs' in the resulting 2D image, and the separation distance between projections is proportional to depth in the volume...
March 20, 2017: Nature Methods
https://www.readbyqxmd.com/read/28318347/teratogenic-hazard-of-bpei-coated-silver-nanoparticles-to-xenopus-laevis
#10
Anita Colombo, Melissa Saibene, Elisa Moschini, Patrizia Bonfanti, Maddalena Collini, Kaja Kasemets, Paride Mantecca
Silver nanoparticles (AgNPs) are among the most exploited antimicrobial agents and are used in many consumer products. Size and surface reactivity are critical physico-chemical properties responsible for NPs toxicity, and surface coatings, often used to functionalize or stabilize AgNPs, can influence their toxic profile and biocompatibility. In the current study the developmental toxicity of 1) negatively charged citrate-coated AgNPs (Cit-AgNPs), 2) positively charged branched polyethylenimine-coated AgNPs (BPEI-AgNPs), and 3) Ag(+) (from 0...
March 20, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28302854/revealing-the-subfemtosecond-dynamics-of-orbital-angular-momentum-in-nanoplasmonic-vortices
#11
G Spektor, D Kilbane, A K Mahro, B Frank, S Ristok, L Gal, P Kahl, D Podbiel, S Mathias, H Giessen, F-J Meyer Zu Heringdorf, M Orenstein, M Aeschlimann
The ability of light to carry and deliver orbital angular momentum (OAM) in the form of optical vortices has attracted much interest. The physical properties of light with a helical wavefront can be confined onto two-dimensional surfaces with subwavelength dimensions in the form of plasmonic vortices, opening avenues for thus far unknown light-matter interactions. Because of their extreme rotational velocity, the ultrafast dynamics of such vortices remained unexplored. Here we show the detailed spatiotemporal evolution of nanovortices using time-resolved two-photon photoemission electron microscopy...
March 17, 2017: Science
https://www.readbyqxmd.com/read/28302649/endothelial-protective-monocyte-patrolling-in-large-arteries-intensified-by-western-diet-and-atherosclerosis
#12
Amado Quintar, Sara McArdle, Dennis Wolf, Alex Marki, Erik Ehinger, Melanie Vassallo, Jacqueline F Miller, Zbigniew Mikulski, Klaus Ley, Konrad Buscher
Rationale: Non-classical mouse monocyte (CX3CR1(high), Ly-6C(low)) patrolling along the vessels of the microcirculation is critical for endothelial homeostasis and inflammation. Due to technical challenges it is currently not established how patrolling occurs in large arteries. Objective: This study was undertaken to elucidate the molecular, migratory and functional phenotype of patrolling monocytes in the high shear and pulsatile environment of large arteries in healthy, hyperlipidemic and atherosclerotic conditions...
March 16, 2017: Circulation Research
https://www.readbyqxmd.com/read/28301654/mapping-of-hemoglobin-in-erythrocytes-and-erythrocyte-ghosts-using-two-photon-excitation-fluorescence-microscopy
#13
Katarina Bukara, Svetlana Jovanic, Ivana T Drvenica, Ana Stancic, Vesna Ilic, Mihailo D Rabasovic, Dejan Pantelic, Branislav Jelenkovic, Branko Bugarski, Aleksandar J Krmpot
No abstract text is available yet for this article.
February 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28295087/high-numerical-aperture-na%C3%A2-%C3%A2-0-92-objective-lens-for-imaging-and-addressing-of-cold-atoms
#14
Carsten Robens, Stefan Brakhane, Wolfgang Alt, Felix Kleißler, Dieter Meschede, Geol Moon, Gautam Ramola, Andrea Alberti
We have designed, built, and characterized a high-resolution objective lens that is compatible with an ultrahigh vacuum environment. The lens system exploits the principle of the Weierstrass sphere solid immersion lens to reach a numerical aperture (NA) of 0.92. Tailored to the requirements of optical lattice experiments, the objective lens features a relatively long working distance of 150 μm. Our two-lens design is remarkably insensitive to mechanical tolerances in spite of the large NA. Additionally, we demonstrate the application of a tapered optical fiber tip, as used in scanning near-field optical microscopy, to measure the point spread function (PSF) of a high NA optical system...
March 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28289377/loss-of-saltation-and-presynaptic-action-potential-failure-in-demyelinated-axons
#15
Mustafa S Hamada, Marko A Popovic, Maarten H P Kole
In cortical pyramidal neurons the presynaptic terminals controlling transmitter release are located along unmyelinated axon collaterals, far from the original action potential (AP) initiation site, the axon initial segment (AIS). Once initiated, APs will need to reliably propagate over long distances and regions of geometrical inhomogeneity like branch points (BPs) to rapidly depolarize the presynaptic terminals and confer temporally precise synaptic transmission. While axon pathologies such as demyelinating diseases are well established to impede the fidelity of AP propagation along internodes, to which extent myelin loss affects propagation along BPs and axon collaterals is not well understood...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28285819/imaging-erk-and-pka-activation-in-single-dendritic-spines-during-structural-plasticity
#16
Shen Tang, Ryohei Yasuda
Extracellular signal-regulated kinase (ERK) and protein kinase A (PKA) play important roles in LTP and spine structural plasticity. While fluorescence resonance energy transfer (FRET)-based sensors for these kinases had previously been developed, they did not provide sufficient sensitivity for imaging small neuronal compartments, such as single dendritic spines in brain slices. Here we improved the sensitivity of FRET-based kinase sensors for monitoring kinase activity under two-photon fluorescence lifetime imaging microscopy (2pFLIM)...
March 22, 2017: Neuron
https://www.readbyqxmd.com/read/28276477/in-vivo-longitudinal-visualization-of-bone-marrow-engraftment-process-in-mouse-calvaria-using-two-photon-microscopy
#17
Viet-Hoan Le, Seunghun Lee, Seungwon Lee, Taejun Wang, Won Hyuk Jang, Yeoreum Yoon, Soonjae Kwon, Hyekang Kim, Seung-Woo Lee, Ki Hean Kim
Intravital microscopy of mouse calvarial bone marrow (BM) is a powerful method for studying hematopoietic stem cells (HSCs) and the BM microenvironment at the cellular level. However, the current method used to access the mouse calvaria allows for only a few imaging times in the same mouse because of scar formation and inflammation induced by multiple surgeries. Longitudinal imaging of the BM may help better understand its microenvironment. In this study, a mouse calvarial window model was developed for longitudinal imaging that involves attaching a cover glass window onto the mouse calvaria and sealing the surrounding exposed area with cyanoacrylate glue and dental cement...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28272392/protein-expression-guided-chemical-profiling-of-living-cells-by-the-simultaneous-observation-of-raman-scattering-and-anti-stokes-fluorescence-emission
#18
Liang-da Chiu, Taro Ichimura, Takumasa Sekiya, Hiroaki Machiyama, Tomonobu Watanabe, Hideaki Fujita, Takeaki Ozawa, Katsumasa Fujita
Our current understanding of molecular biology provides a clear picture of how the genome, transcriptome and proteome regulate each other, but how the chemical environment of the cell plays a role in cellular regulation remains much to be studied. Here we show an imaging method using hybrid fluorescence-Raman microscopy that measures the chemical micro-environment associated with protein expression patterns in a living cell. Simultaneous detection of fluorescence and Raman signals, realised by spectrally separating the two modes through the single photon anti-Stokes fluorescence emission of fluorescent proteins, enables the accurate correlation of the chemical fingerprint of a specimen to its physiological state...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28261331/a-biocompatible-macromolecular-two-photon-initiator-based-on-hyaluronan
#19
Maximilian Tromayer, Peter Gruber, Marica Markovic, Arnulf Rosspeintner, Eric Vauthey, Heinz Redl, Aleksandr Ovsianikov, Robert Liska
The possibility of the direct encapsulation of living cells via two-photon induced photopolymerization enables the microfabrication of hydrogel scaffolds with high initial cell loadings and intimate matrix-cell contact. While highly efficient water-soluble two-photon initiators based on benzylidene ketone dyes have been developed, they exhibit considerable cyto- and phototoxicity. To address the problem of photoinitiator migration from the extracellular matrix into the cytosol, a two-photon initiator bound to a polymeric hyaluronan backbone (HAPI) was synthesized in this work...
January 14, 2017: Polymer Chemistry
https://www.readbyqxmd.com/read/28261060/dendritic-spikes-in-sensory-perception
#20
REVIEW
Satoshi Manita, Hiroyoshi Miyakawa, Kazuo Kitamura, Masanori Murayama
What is the function of dendritic spikes? One might argue that they provide conditions for neuronal plasticity or that they are essential for neural computation. However, despite a long history of dendritic research, the physiological relevance of dendritic spikes in brain function remains unknown. This could stem from the fact that most studies on dendrites have been performed in vitro. Fortunately, the emergence of novel techniques such as improved two-photon microscopy, genetically encoded calcium indicators (GECIs), and optogenetic tools has provided the means for vital breakthroughs in in vivo dendritic research...
2017: Frontiers in Cellular Neuroscience
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