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https://www.readbyqxmd.com/read/25123395/validation-of-dna-promoter-hypermethylation-biomarkers-in-breast-cancer-a-short-report
#1
Jolien S de Groot, Xiaojuan Pan, Jan Meeldijk, Elsken van der Wall, Paul J van Diest, Cathy B Moelans
PURPOSE: DNA promoter hypermethylation of tumor suppressor genes is known to occur early in cancer development, including breast cancer. To improve early breast cancer detection, we aimed to investigate whether the identification of DNA promoter hypermethylation might be of added value. METHODS: The methylation status of a panel of 19 candidate genes (AKR1B1, ALX1, ARHGEF7, FZD10, GHSR, GPX7, GREM1, GSTP1, HOXD1, KL, LHX2, MAL, MGMT, NDRG2, RASGRF2, SFRP1, SFRP2, TM6SF1 and TMEFF2) was determined in formalin-fixed paraffin-embedded normal breast and breast cancer tissue samples using gel-based methylation-specific PCR (MSP)...
August 2014: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/24628919/dissecting-the-signaling-pathways-associated-with-the-oncogenic-activity-of-mlk3-p252h-mutation
#2
Sérgia Velho, Ana Pinto, Danilo Licastro, Maria José Oliveira, Filipa Sousa, Elia Stupka, Raquel Seruca
BACKGROUND: MLK3 gene mutations were described to occur in about 20% of microsatellite unstable gastrointestinal cancers and to harbor oncogenic activity. In particular, mutation P252H, located in the kinase domain, was found to have a strong transforming potential, and to promote the growth of highly invasive tumors when subcutaneously injected in nude mice. Nevertheless, the molecular mechanism underlying the oncogenic activity of P252H mutant remained elusive. METHODS: In this work, we performed Illumina Whole Genome arrays on three biological replicas of human HEK293 cells stably transfected with the wild-type MLK3, the P252H mutation and with the empty vector (Mock) in order to identify the putative signaling pathways associated with P252H mutation...
2014: BMC Cancer
https://www.readbyqxmd.com/read/19134005/inverse-correlation-of-the-up-regulation-of-fzd10-expression-and-the-activation-of-beta-catenin-in-synchronous-colorectal-tumors
#3
Satoshi Nagayama, Eiji Yamada, Yoshiki Kohno, Tomoki Aoyama, Chikako Fukukawa, Hajime Kubo, Go Watanabe, Toyomasa Katagiri, Yusuke Nakamura, Yoshiharu Sakai, Junya Toguchida
We investigated the immunohistochemical expression patterns of Frizzled homolog 10 (FZD10), a cell-surface receptor for molecules in the Wnt pathway, in tissue samples derived from 104 patients with colorectal cancers (CRCs). There was no immunoreactivity for FZD10 in normal colonic mucosa, and only tumor cells in polyps and CRC tissues showed spotted immunostaining patterns in apical sides of the cytoplasm. In metastatic liver lesions, tumor cells showed cytoplasmic immunostaining similar to primary lesions, whereas normal liver parenchyma showed almost no immunostaining...
March 2009: Cancer Science
https://www.readbyqxmd.com/read/18680391/isolation-and-identification-of-cancer-stem-like-cells-in-esophageal-carcinoma-cell-lines
#4
Dingzhi Huang, Quanli Gao, Liping Guo, Chunpeng Zhang, Wei Jiang, Hongxia Li, Jing Wang, Xiaohong Han, Yuankai Shi, Shih Hsin Lu
Side population (SP) cells may play an important role in tumorigenesis and cancer therapy. We isolate and identify the cancer stem-like cells in human esophageal carcinoma (EC) cell lines, EC9706 and EC109 cells labeled with Hoechst 33342. Both the cell lines contained SP cells, and the cells that had the strongest dye efflux activity ("Tip"-SP cell) in EC9706 had higher clone formation efficiency than non-SP cells. When transplanted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, "Tip"-SP cells showed at least 50 times higher tumorigenicity than non-SP cells...
April 2009: Stem Cells and Development
https://www.readbyqxmd.com/read/18567805/secreted-frizzled-related-protein-4-regulates-two-wnt7a-signaling-pathways-and-inhibits-proliferation-in-endometrial-cancer-cells
#5
Kendra S Carmon, David S Loose
In the endometrium, hormonal effects on epithelial cells are often elicited through stromal hormone receptors via unknown paracrine mechanisms. Several lines of evidence support the hypothesis that Wnts participate in stromal-epithelial cell communication. Wnt7a is expressed in the luminal epithelium, whereas the extracellular modulator of Wnt signaling, secreted frizzled-related protein 4 (SFRP4), is localized to the stroma. Studies have reported that SFRP4 expression is significantly decreased in endometrial carcinoma and that both SFRP4 and Wnt7a genes are differentially regulated in response to estrogenic stimuli...
June 2008: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/18057535/gpr87-is-an-overexpressed-g-protein-coupled-receptor-in-squamous-cell-carcinoma-of-the-lung
#6
Mathias Gugger, Richard White, Susan Song, Bea Waser, Renzo Cescato, Pierre Rivière, Jean Claude Reubi
Lung cancer is the leading cause of cancer death worldwide. The overall 5-year survival after therapy is about 16% and there is a clear need for better treatment options, such as therapies targeting specific molecular structures. G-protein coupled receptors (GPCRs), as the largest family of cell surface receptors, represent an important group of potential targets for diagnostics and therapy. We therefore used laser capture microdissection and GPCR-focused Affymetrix microarrays to examine the expression of 929 GPCR transcripts in tissue samples of 10 patients with squamous cell carcinoma and 7 with adenocarcinoma in order to identify novel targets in non-small cell lung carcinoma (NSCLC)...
2008: Disease Markers
https://www.readbyqxmd.com/read/17873379/networking-of-wnt-fgf-notch-bmp-and-hedgehog-signaling-pathways-during-carcinogenesis
#7
REVIEW
Masaru Katoh
The biological functions of some orthologs within the human genome and model-animal genomes are evolutionarily conserved, but those of others are divergent due to protein evolution and promoter evolution. Because WNT signaling molecules play key roles during embryogenesis, tissue regeneration and carcinogenesis, the author's group has carried out a human WNT-ome project for the comprehensive characterization of human genes encoding WNT signaling molecules. From 1996 to 2002, we cloned and characterized WNT2B/WNT13, WNT3, WNT3A, WNT5B, WNT6, WNT7B, WNT8A, WNT8B, WNT9A/WNT14, WNT9B/WNT14B, WNT10A, WNT10B, WNT11, FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD10, FRAT1, FRAT2, NKD1, NKD2, VANGL1, RHOU/ARHU, RHOV/ARHV, GIPC2, GIPC3, FBXW11/betaTRCP2, SOX17, TCF7L1/TCF3, and established a cDNA-PCR system for snap-shot and dynamic analyses on the WNT-transcriptome...
January 2007: Stem Cell Reviews
https://www.readbyqxmd.com/read/16465433/redundant-expression-of-canonical-wnt-ligands-in-human-breast-cancer-cell-lines
#8
COMPARATIVE STUDY
Khemais Benhaj, Kamil Can Akcali, Mehmet Ozturk
Human breast cancer displays nuclear accumulation of beta-catenin and induction of cyclin D1 expression, which suggests that canonical Wnt/beta-catenin signaling is activated. In other cancers, the activation of canonical wnt/beta-catenin signaling is associated with APC, CTNNB1 or AXIN1 mutations. However, these mutations are rare or absent in breast cancer. In search of alternative mechanisms, we performed comprehensive expression analysis of Wnt signaling molecules, including 19 Wnt ligands, ten Frizzled receptors, two co-receptors and four Lef/TCF transcription factors in immortalized normal human mammary epithelial cells (HMEC) and six breast cancer cell lines...
March 2006: Oncology Reports
https://www.readbyqxmd.com/read/16211313/comparative-genomics-on-ror1-and-ror2-orthologs
#9
COMPARATIVE STUDY
Masuko Katoh, Masaru Katoh
Transmembrane proteins with extracellular Frizzled domain, such as ROR1, ROR2, MUSK, MFRP, FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9 and FZD10, are key molecules for WNT signaling network. Here, comparative integromics analyses on ROR1 and ROR2 orthologs were performed by using bioinformatics. Zebrafish ror2 gene, consisting of nine exons, was identified within CR-450684.3 genome sequence. CV490605.1 EST corresponded to the 5'-end of zebrafish ror2 mRNA, and BM533602.1 EST corresponded to the 3'-end...
November 2005: Oncology Reports
https://www.readbyqxmd.com/read/15754011/comparative-genomics-on-wnt8a-and-wnt8b-genes
#10
COMPARATIVE STUDY
Masuko Katoh, Masaru Katoh
WNT family ligands transduce signals through FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9, FZD10, LRP5, LRP6, ROR1, ROR2 and RYK. WNT1, WNT2, WNT2B, WNT3, WNT3A, WNT8A, WNT8B, WNT10A and WNT10B are canonical WNTs to activate WNT - beta-catenin pathway. Human WNT8A mRNA is expressed in NT2 cells with neuronal differentiation potential, while human WNT8B mRNA in diffuse type gastric cancer. Here, we identified and characterized the rat Wnt8a and Wnt8b genes by using bioinformatics. The rat Wnt8a gene, consisting of six exons, was located within AC134361...
April 2005: International Journal of Oncology
https://www.readbyqxmd.com/read/12239632/expression-of-wnt7a-in-human-normal-tissues-and-cancer-and-regulation-of-wnt7a-and-wnt7b-in-human-cancer
#11
Hiroyuki Kirikoshi, Masaru Katoh
WNT signals are transduced through seven-transmembrane-type WNT receptors encoded by Frizzled (FZD) genes to the beta-catenin - TCF pathway, the JNK pathway or the Ca2+-releasing pathway. WNT signaling molecules are potent targets for diagnosis of cancer (susceptibility, metastasis, and prognosis), for prevention and treatment of cancer, and for regenerative medicine or tissue engineering. We have so far cloned and characterized human WNT signaling molecules WNT2B/WNT13, WNT3, WNT3A, WNT5B, WNT6, WNT7B, WNT8A, WNT8B, WNT10A, WNT10B, WNT11, WNT14, WNT14B/WNT15, FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD10, FRAT1, FRAT2, NKD1, NKD2, VANGL1/STB2, ARHU/WRCH1, ARHV/WRCH2, GIPC2, GIPC3, betaTRCP2/FBXW1B, SOX17, and TCF-3 using bioinformatics, cDNA-library screening, and cDNA-PCR...
October 2002: International Journal of Oncology
https://www.readbyqxmd.com/read/11956659/frequent-up-regulation-of-wnt5a-mrna-in-primary-gastric-cancer
#12
Tetsuroh Saitoh, Tetsuya Mine, Masaru Katoh
WNT signal is transduced to the beta-catenin - TCF pathway, the JNK pathway, or the Ca2+-releasing pathway through seven-transmembrane-type WNT receptors encoded by Frizzled genes (FZD1-FZD10). We have previously cloned and characterized human WNT2B/WNT13, WNT3, WNT3A, WNT5B, WNT6, WNT7B, WNT8A, WNT8B, WNT10A, WNT10B, WNT11, WNT14, and WNT14B/WNT15 by using bioinformatics, cDNA-library screening, and cDNA-PCR. Here, we investigated expression of human WNT5A mRNA in various normal tissues, 66 primary tumors derived from various tissues, and 15 human cancer cell lines...
May 2002: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/11956592/molecular-cloning-and-characterization-of-wrch2-on-human-chromosome-15q15
#13
Masaru Katoh
WNT signals are transduced to the JNK pathway, the Ca2+-releasing pathway, or the beta-catenin - TCF pathway through seven-transmembrane-type WNT receptors encoded by Frizzled genes (FZD1-FZD10). WRCH1/ARHV and CDC42 are potentially implicated in the WNT-JNK pathway. Here, WRCH2/ARHV cDNAs were isolated by using bioinformatics and cDNA-PCR. WRCH2 gene, consisting of at least 3 exons, encoded a 236-amino-acid protein with proline-rich domain and GTPase domain. WRCH2 was homologous to WRCH1 (55.4% total-amino-acid identity) and CDC42 (43...
May 2002: International Journal of Oncology
https://www.readbyqxmd.com/read/11894124/expression-of-wrch1-in-human-cancer-and-down-regulation-of-wrch1-by-beta-estradiol-in-mcf-7-cells
#14
COMPARATIVE STUDY
Hiroyuki Kirikoshi, Masaru Katoh
Secreted-type glycoprotein WNTs bind to seven-transmembrane-type WNT receptors encoded by Frizzled genes (FZD1-FZD10) to transduce signals to the beta-catenin--TCF pathway, the JNK pathway, or the Ca(2+)-releasing pathway. Wrch1 gene is a down-stream target gene of Wnt1 in C57MG cells, and encodes a Cdc42-related GTPase with the potential to activate the JNK pathway. Here, we isolated human WRCH1 cDNAs (accession no. AB074878) from gastric cancer cell lines OKAJIMA, MKN7, MKN28, MKN45, MKN74, and KATO-III, all of which showed a nucleotide substitution (343 C-->T) without amino-acid substitution compared with WRCH1 cDNA isolated by another group...
April 2002: International Journal of Oncology
https://www.readbyqxmd.com/read/11786918/frizzled-10-up-regulated-in-primary-colorectal-cancer-is-a-positive-regulator-of-the-wnt-beta-catenin-tcf-signaling-pathway
#15
Harumi Terasaki, Tetsuroh Saitoh, Koichiro Shiokawa, Masaru Katoh
WNT signaling pathway is implicated in embryogenesis as well as in carcinogenesis. We have previously cloned and characterized Frizzled-1 (FZD1), FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, and FZD10, encoding seven-transmembrane-type WNT receptors. Here, expression of FZD10 mRNA in various types of human cancer and effects of FZD10 mRNA microinjection into Xenopus early embryos were investigated. Northern blot analyses revealed relatively high-level expression of 4.0-kb FZD10 mRNA in cervical cancer cell lines HeLa S3, SKG-I, SKG-IIIa, and in a glioblastoma cell line A-172...
February 2002: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/11743650/up-regulation-of-frizzled-10-fzd10-by-beta-estradiol-in-mcf-7-cells-and-by-retinoic-acid-in-nt2-cells
#16
Tetsuroh Saitoh, Tetsuya Mine, Masaru Katoh
Frizzled (FZD) genes encode receptors for WNTs, which play key roles in carcinogenesis and embryogenesis. We have previously cloned the FZD10 gene, and demonstrated up-regulation of FZD10 mRNA in the cervical cancer cell line HeLa S3, gastric cancer cell lines TMK1 and MKN74, and 4 cases out of 10 cases of primary gastric cancer. Here, effects of beta-estradiol, retinoic acid, and inflammatory cytokines on expression of FZD10 mRNA in human cancer cell lines were investigated. FZD10 mRNA was undetectable in MCF-7 cells derived from breast cancer, and was significantly up-regulated by beta-estradiol in MCF-7 cells with a peak at 24 h after treatment...
January 2002: International Journal of Oncology
https://www.readbyqxmd.com/read/11562753/expression-profiles-of-10-members-of-frizzled-gene-family-in-human-gastric-cancer
#17
H Kirikoshi, H Sekihara, M Katoh
Frizzled (FZD) genes encode seven-transmembrane type WNT receptors, which are implicated in carcinogenesis and embryogenesis. We have previously cloned and characterized FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, and FZD10. Here, we investigated expression profiles of all members of the FZD gene family in human gastric cancer. FZD mRNAs were detected by one-step cDNA-PCR. Specificity of cDNA-PCR was confirmed by nucleotide sequence analyses of cDNA-PCR products. Among seven gastric cancer cell lines, FZD7 was up-regulated in MKN7, which was consistent with a previous report...
October 2001: International Journal of Oncology
https://www.readbyqxmd.com/read/10544037/molecular-cloning-and-characterization-of-human-frizzled-4-on-chromosome-11q14-q21
#18
H Kirikoshi, N Sagara, J Koike, K Tanaka, H Sekihara, M Hirai, M Katoh
The WNT receptors, encoded by the Frizzled genes, are implicated in a variety of cellular processes such as cell fate determination, cell polarity control, and malignant transformation. Human Frizzled-4 (FZD4) cDNAs have been cloned and characterized. FZD4 spans a total of 7392 nucleotides and encodes a 537-amino-acid protein with the N-terminal cysteine-rich domain, seven transmembrane domains, and the C-terminal S/T-X-V motif. The FZD4 mRNA of 7.7 kb in size were detected almost ubiquitously in normal human tissues and larger amounts in fetal kidney, adult heart, skeletal muscle, and ovary...
November 2, 1999: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/10448064/molecular-cloning-of-frizzled-10-a-novel-member-of-the-frizzled-gene-family
#19
J Koike, A Takagi, T Miwa, M Hirai, M Terada, M Katoh
The Frizzled genes encode WNT receptors. Frizzled-10 (FZD10), a novel member of the Frizzled gene family, has been cloned and characterized. Nucleotide sequence analysis showed that human FZD10 gene encodes a seven-transmembrane-receptor of 581 amino acids, with the N-terminal cysteine-rich domain and the C-terminal Ser/Thr-Xxx-Val motif. Larger amounts of FZD10 mRNA, 4.0 kb in size, were detected in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, FZD10 mRNA was abundant in the cerebellum...
August 19, 1999: Biochemical and Biophysical Research Communications
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