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https://www.readbyqxmd.com/read/29138357/tadalafil-a-long-acting-phosphodiesterase-inhibitor-promotes-bone-marrow-stem-cell-survival-and-their-homing-into-ischemic-myocardium-for-cardiac-repair
#1
Ibrahim Elmadbouh, Muhammad Ashraf
The aim was to evaluate the tadalafil-mediated effects at molecular level on bone marrow-derived mesenchymal stem cells (MSCs) survival and their homing into the infarcted hearts to promote cardiac repair and improve function. MSCs were pretreated in vitro with inhibitors of PKG, MAPK, FasL, nitric oxide synthase (NOS) (L-NAME), CXCR4 (AMD3100), or miR-21 inhibitors (+/-luciferase construction +/-Fas) prior to tadalafil treatment for 2 h. These MSCs were then subjected to H2O2 stress to assess their injury...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29130799/allergen-immunotherapy-modulates-sensitivity-of-treg-cells-to-apoptosis-in-a-rat-model-of-allergic-asthma
#2
Ankur Datta, Saibal Moitra, Prasanta K Das, Somnath Mondal, Sk Md Omar Faruk, Iman Hazra, Santanu K Tripathi, Swapna Chaudhuri
AIM: To study the apoptosis of Foxp3(+) Treg cells following Alstonia scholaris pollen sensitization-challenge and following allergen immunotherapy. MATERIALS & METHODS: Wistar rats were sensitized-challenged with Alstonia scholaris pollen and were further given intranasal immunotherapy. For the analysis of the apoptotic proteins on Treg cells by flow cytometry, multiple gating procedures were followed. RESULTS: Allergen sensitization-challenge increases Annexin-V, Fas, FasL, caspases-8, 9, 3 cytochrome-C, APAF-1, Bax, perforin-1 and granzyme-B on Treg cells which is decreased following intranasal immunotherapy...
November 2017: Immunotherapy
https://www.readbyqxmd.com/read/29128795/chemosensitive-effects-of-astragaloside-iv-in-osteosarcoma-cells-via-induction-of-apoptosis-and-regulation-of-caspase-dependent-fas-fasl-signaling
#3
Tongzhou Hu, Zhenghua Fei, Nan Wei
BACKGROUND: The response of conventional chemotherapy for osteosarcoma treatment is usually poor, and chemotherapy-related severe side effects and drug resistance remain a problem. Abundant evidence has shown that Astragaloside IV, extracted from Astragalus membranaceus Bunge, strongly inhibits the growth of many carcinomas. We aimed to investigate the chemosensitive effects of Astragaloside IV in osteosarcoma in vitro and in vivo. METHODS: Human osteosarcoma cell lines MG-63 and 143B, and BALB/c nu/nu mice xenograft were used...
July 5, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29126851/ccr6-signaling-inhibits-suppressor-function-of-induced-treg-during-gut-inflammation
#4
Neeraja Kulkarni, Heikrujam Thoihen Meitei, Sandip Ashok Sonar, Praveen Kumar Sharma, Vikkarasan Rehman Mujeeb, Sharad Srivastava, Ramanamurthy Boppana, Girdhari Lal
CCR6 is a G protein-coupled receptor (GPCR) that binds to a specific chemokine, CCL20. The role of CCR6-CCL20 is very well studied in the migration of immune cells, but the non-chemotaxis functions of CCR6 signaling were not known. Here, we show that during gut inflammation, the frequency of Foxp3(+)CD4(+) T cells (Tregs) reduced in the secondary lymphoid tissues and CCR6(+) Tregs enhanced the expression of RORγt. The peripheral blood mononuclear cells (PBMCs) of ulcerative colitis (UC) patients showed lower percentages of Foxp3(+)CD4(+) T cells, as compared to healthy individuals, with CCR6(+) Tregs showing higher RORγt expression as compared to CCR6(-)Tregs...
November 7, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/29115636/fstl1-suppresses-tumor-cell-proliferation-invasion-and-survival-in-non-small-cell-lung-cancer
#5
Xiaolei Ni, Xiaoming Cao, Yongquan Wu, Jian Wu
Follistatin like-1 (FSTL1) is a secreted glycoprotein involved in a series of physiological and pathological processes. However, its contribution to the development of cancer, especially the pathogenesis of NSCLC, remains to be elucidated. We explored the expression, function, and molecular mechanism of FSTL1 in NSCLC. In this study, we detected the expression of FSTL1 in a panel of NSCLC cell lines and lung normal epithelial cell line by qRT-PCR and western blot analysis and found that FSTL1 was downregulated in NSCLC cells compared with normal control...
October 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29101404/autoreactive-t-cells-in-immunopathogenesis-of-tb-associated-uveitis
#6
Ravichandra Tagirasa, Shaifaly Parmar, Manas R Barik, Satish Devadas, Soumyava Basu
Purpose: Intraocular inflammation in tuberculosis-associated uveitis (TBU) is usually widespread, and responds unpredictably to treatment. Herein, we analyze the intraocular T-cell response in TBU for its surface phenotype, antigenic specificity, and functional characteristics to explain the above observations. Methods: We isolated T cells from vitreous humor samples of patients with TBU and non-TB uveitis (controls). These were directly stained for surface markers CD4, CD8, CD45RO, CD45RA, CCR7, as well as intracellular cytokines IFN-γ, TNF-α, and IL-17 and analyzed on flow cytometry...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29089545/activation-of-fas-fasl-pathway-and-the-role-of-c-flip-in-primary-culture-of-human-cholangiocarcinoma-cells
#7
Gianluca Carnevale, Guido Carpino, Vincenzo Cardinale, Alessandra Pisciotta, Massimo Riccio, Laura Bertoni, Lara Gibellini, Sara De Biasi, Lorenzo Nevi, Daniele Costantini, Diletta Overi, Andrea Cossarizza, Anto de Pol, Eugenio Gaudio, Domenico Alvaro
Intrahepatic cholangiocarcinoma (iCCA) represents a heterogeneous group of malignancies emerging from the biliary tree, often in the context of chronic bile ducts inflammation. The immunological features of iCCA cells and their capability to control the lymphocytes response have not yet been investigated. The aims of the present study were to evaluate the interaction between iCCA cells and human peripheral blood mononuclear cells (PBMCs) and the role of Fas/FasL in modulating T-cells and NK-cells response after direct co-culture...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29083508/innate-immunity-protein-tag7-pgrp-s-activates-lymphocytes-capable-of-fasl-fas-dependent-contact-killing-of-virus-infected-cells
#8
Tatiana N Sharapova, Olga K Ivanova, Vladimir S Prasolov, Elena A Romanova, Lidia P Sashchenko, Denis V Yashin
The innate immunity protein Tag7 (PGRP-S, PGLYRP1) is involved in antimicrobial and antitumor defense. As shown in our previous studies, Tag7 specifically interacts with the major heat shock protein Hsp70 to form a stable Tag7-Hsp70 complex with cytotoxic activity against tumor cells. A stable complex of Tag7 with the calcium-binding protein Mts1 (S100A4) stimulates migration of lymphocytes. Moreover, Tag7 can activate cytotoxic lymphocytes that recognize and kill HLA-negative tumor cells. Here, we have shown that Tag 7 treatment of human peripheral blood mononuclear cells (PBMCs) results in activation of different cytotoxic lymphocyte populations-natural killer (NK) cells and CD8(+) NKG2D(+) T lymphocytes-that kill Moloney murine leukemia virus (MMLV) infected SC-1 cells using different mechanisms of cell death induction...
October 30, 2017: IUBMB Life
https://www.readbyqxmd.com/read/29079852/exopolysaccharides-from-lactobacillus-plantarum-ncu116-induce-c-jun-dependent-fas-fasl-mediated-apoptosis-via-tlr2-in-mouse-intestinal-epithelial-cancer-cells
#9
Xingtao Zhou, Tao Hong, Qiang Yu, Shaoping Nie, Deming Gong, Tao Xiong, Mingyong Xie
Exopolysaccharides (EPS) from lactic acid bacteria (LAB) have been reported to play vital parts in the modulation of cell-cycle and apoptosis in cancer cells. However, the mechanisms by which EPS regulate the proliferation and apoptosis of cancer cells remain incompletely understood. We thus used different cancer cells to evaluate the anticancer ability and to investigate the underlying molecular mechanism of EPS from Lactobacillus plantarum NCU116 (EPS116). Our studies showed that EPS116 inhibited the proliferation of cancer cells in a cell type manner, and remarkably repressed the growth and survival of CT26 through induction of apoptosis...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29079711/histone-deacetylase-inhibition-enhances-the-antitumor-activity-of-a-mek-inhibitor-in-lung-cancer-cells-harboring-ras-mutations
#10
Tadaaki Yamada, Joseph M Amann, Azusa Tanimoto, Hirokazu Taniguchi, Takehito Shukuya, Cynthia Timmers, Seiji Yano, Konstantin Shilo, David P Carbone
Non-small-cell lung cancer (NSCLC) can be identified by precise molecular subsets based on genomic alterations that drive tumorigenesis and include mutations in EGFR, KRAS, and various ALK fusions. However, despite effective treatments for EGFR and ALK, promising therapeutics have not been developed for patients with KRAS mutations. It has been reported that one way the RAS-ERK pathway contributes to tumorigenesis is by affecting stability and localization of FOXO3a protein, an important regulator of cell death and the cell cycle...
October 27, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29072392/genetic-polymorphisms-of-fas-fasl-promoter-associated-with-hepatitis-c-cirrhosis-and-hcc
#11
Sally Abed, Mohamed El-Dosoky, Maysaa El Sayed Zaki, Mohamed EL-Shafey
Aim: The present study was performed to determine any associations of genetic polymorphisms of Fas/FasL promoter regions, at Fas670 and Fas1377 and FasL844, with hepatitis C cirrhosis and HCC, with a focus on severity of disease. Methods: Totals of 120 patients with cirrhosis and 101 with hepatocellular carcinoma (HCC) were enrolled. All had chronic HCV infection as indicated by positive anti-HCV antibodies and positive HCV RNA on real time PCR. One hundred healthy control subjects were also included in the study...
October 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29048613/mir-768-3p-is-involved-in-the-proliferation-invasion-and-migration-of-non-small-cell-lung-carcinomas
#12
Zuozhou Xie, Weiqiang Chen, Yinghua Chen, Xiang Wang, Wenyong Gao, Yi Liu
Altered microRNA expression has been found to be a common feature of several cancers, including lung carcinomas. However, the possible roles of miR-768-3p in the pathological changes of lung carcinomas are still unknown. The aim of the present study was to investigate the expression and possible effects of miR-768-3p in human non-small cell lung carcinomas (NSCLC). Eighty-three NSCLC patients attending the clinic of Kunming Hospital were invited to participate in the study. Their tumor samples were obtained for qRT-PCR analysis...
September 22, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29045972/-acetylsalicylic-acid-treatment-enhanced-immunomodulatory-function-of-mesenchymal-stem-cells-derived-from-gingiva
#13
R L Yang, T T Yu, Y H Zhou
OBJECTIVE: To analyze the role of acetylsalicylic acid (ASA) in immunomodulation of mesenchymal stem cells derived from gingiva (GMSCs), and to explore the role of ASA in enhancing the immumomodulation of GMSCs and the capacity of GMSCs to treat immune disorders and the underlying mechanism. METHODS: Flow cytometry analysis were used to analyze the role of ASA in the expression of stem cells surface markers CD146, CD105, CD90, CD34 and CD45 in GMSCs,and the GMSCs proliferation was analyzed by 5-bromo-2-deoxyuridine (BrdU) staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29038485/a-versatile-pretargeting-approach-for-tumour-selective-delivery-and-activation-of-tnf-superfamily-members
#14
Yuan He, Peter E van Bommel, Douwe F Samplonius, Edwin Bremer, Wijnand Helfrich
TNFR superfamily (TNFRSF) members have important immunoregulatory functions and are of clear interest for cancer immunotherapy. Various TNFRSF agonists have been clinically evaluated, but have met with limited efficacy and/or toxicity. Recent insights indicate that 'first-generation' TNFRSF agonists lack efficacy as they do not effectively cross-link their corresponding receptor. Reversely, ubiquitous TNFRSF receptor(s) cross-linking by CD40 and Fas agonistic antibodies resulted in dose-limiting liver toxicity...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29028819/the-anti-myeloma-activity-of-bone-morphogenetic-protein-2-predominantly-relies-on-the-induction-of-growth-arrest-and-is-apoptosis-independent
#15
Charlotte Lagler, Mohamed El-Mesery, Alexander Christian Kübler, Urs Dietmar Achim Müller-Richter, Thorsten Stühmer, Joachim Nickel, Thomas Dieter Müller, Harald Wajant, Axel Seher
Multiple myeloma (MM), a malignancy of the bone marrow, is characterized by a pathological increase in antibody-producing plasma cells and an increase in immunoglobulins (plasmacytosis). In recent years, bone morphogenetic proteins (BMPs) have been reported to be activators of apoptotic cell death in neoplastic B cells in MM. Here, we use bone morphogenetic protein 2 (BMP2) to show that the "apoptotic" effect of BMPs on human neoplastic B cells is dominated by anti-proliferative activities and cell cycle arrest and is apoptosis-independent...
2017: PloS One
https://www.readbyqxmd.com/read/29021368/spontaneous-pmn-apoptosis-in-type-2-diabetes-and-the-impact-of-periodontitis
#16
Aggasit Manosudprasit, Alpdogan Kantarci, Hatice Hasturk, Danielle Stephens, Thomas E Van Dyke
The purpose of this study was to test the hypothesis that peripheral blood neutrophils (PMN) exhibit delayed spontaneous apoptosis in individuals with diabetes mellitus type 2 (T2DM) and that the delay is exacerbated further among people who coexpress chronic periodontitis (CP). Seventy-three individuals were enrolled, including those with T2DM (n = 16), CP (n = 15), T2DM + CP (n = 21), and healthy volunteers (n = 21). PMN apoptosis was determined by flow cytometry using TUNEL and Annexin V assays. The activity of caspase-3, -8, and -9 was measured by colorimetric assay...
October 11, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28985848/transforming-growth-factor-%C3%AE-1-and-fas-ligand-synergistically-enhance-immune-tolerance-in-dendritic-cells-in-liver-transplantation
#17
Minglian Qiu, Yujuan Chen, Lihong Chen, Jinhua Zeng, Jingfeng Liu
BACKGROUND: Long-term survival of patients following liver transplantation can be achieved by application of genetically modified, immune tolerogenic immature dendritic cells (imDCs) to overcome allograft-induced acute cellular rejection, a major cause of death. In this study, using a rat model of liver transplantation, we determined whether cotransfection of transforming growth factor β1 (TGF-β1) and Fas ligand (FasL) in imDCs synergistically enhances immune tolerance. MATERIALS AND METHODS: We first determined the immune tolerogenic effects of TGF-β1 and FasL independently or together in imDCs by measuring the levels of CD86 and CD80 and by assessing T-cell proliferation using mixed lymphocyte reaction tests...
October 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28984341/immunohistochemical-analysis-of-the-extrinsic-apoptosis-process-in-the-non-neoplastic-and-neoplastic-prostate
#18
Ayşe Burcu Ileri, Reşit Doğan Köseoğlu, Fatma Markoç, İlker Etikan, Doğan Atilgan, Faik Alev Deresoy
OBJECTIVE: Deviations in the apoptotic process have been demonstrated in prostate carcinogenesis. We aimed to evaluate especially the process of extrinsic apoptosis in the spectrum of neoplastic lesions of the prostate epithelium so as to reveal the variations in the apoptotic process. MATERIAL AND METHOD: The study included 20 benign prostatic hyperplasia, 8 high-grade prostatic intraepithelial neoplasia and 82 prostatic carcinoma patients. Immunohistochemistry was performed on sections obtained from materials of suprapubic prostatectomy, tru-cut biopsy, transurethral resection and radical prostatectomy...
October 6, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28978315/resveratrol-enhances-anticancer-effects-of-paclitaxel-in-hepg2-human-liver-cancer-cells
#19
Qin Jiang, Manyi Yang, Zhan Qu, Jixiang Zhou, Qi Zhang
BACKGROUND: The aim of this in vitro study was to measure the enhanced anticancer effects of Res (resveratrol) on PA (paclitaxel) in HepG2 human liver cancer cells. METHODS: The MTT (thiazolyl blue tetrazolium bromide, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), flow cytometry, qPCR (real-time quantitative polymerase chain reaction) and western blot assay were used for cells growth inhibitory effects, cells apoptosis (DNA content of sub-G1), mRNA and protein expressions, respectively...
October 4, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28977785/innate-immunity-protein-tag7-induces-3-distinct-populations-of-cytotoxic-cells-that-use-different-mechanisms-to-exhibit-their-antitumor-activity-on-human-leukocyte-antigen-deficient-cancer-cells
#20
Tatiana N Sharapova, Olga K Ivanova, Natalia V Soshnikova, Elena A Romanova, Lidia P Sashchenko, Denis V Yashin
The search for new immune response mechanisms capable of controlling immune-evasive tumor cells devoid of the MHC antigen is a challenging task for immunologists. In this study, we found that the treatment of human peripheral blood lymphocytes with the innate immunity protein Tag7 (PGRP-S, PGLYRP1) induces differentiation of the populations of NK (natural killer) cells and CD8+ and CD4+ T lymphocytes that are cytotoxic for human leukocyte antigen-negative tumor cells. These populations employ different mechanisms of tumor cell lysis (based on the release of granzymes in the case of NK cells and on the FasL-Fas interaction in the case of CD8+ and CD4+ T lymphocytes) and induce different death pathways (apoptosis or necroptosis) in tumor cells...
October 4, 2017: Journal of Innate Immunity
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