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https://www.readbyqxmd.com/read/28098878/early-growth-response-3-inhibits-growth-of-hepatocellular-carcinoma-cells-via-upregulation-of-fas-ligand
#1
Shujuan Zhang, Chao Xia, Cong Xu, Jing Liu, Han Zhu, Ya Yang, Fei Xu, Juanfeng Zhao, Ying Chang, Qiu Zhao
Hepatocellular carcinoma (HCC) is a prevalent malignancy with aggressive biological behavior and poor prognosis. Early growth response 3 (EGR3) is a zinc finger transcription factor, and has been studied primarily in the context of neurodevelopment, autoimmunity, inflammation and angiogenesis. Accumulating evidence indicates that EGR3 is a novel suppressor gene of tumor initiation and progression in certain cancer events, but little work has been carried out in exploring the relationship between EGR3 and HCC growth...
January 18, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28094869/loss-of-fas-fasl-signaling-does-not-reduce-apoptosis-in-sharpin-null-mice
#2
Christopher S Potter, Kathleen A Silva, Victoria E Kennedy, Timothy M Stearns, Harm HogenEsch, John P Sundberg
Mice with mutations in SHANK-associated RH domain interactor (Sharpin) develop a hypereosinophilic auto-inflammatory disease known as chronic proliferative dermatitis. Affected mice have increased apoptosis in the keratinocytes of the skin, esophagus, and forestomach driven by extrinsic TNF receptor mediated apoptotic signaling pathways. FAS receptor signaling is an extrinsic apoptotic signaling mechanism frequently involved in inflammatory skin diseases. Compound mutations in Sharpin and Fas or Fasl were created to determine if these death domain proteins influenced the cutaneous phenotype in Sharpin null mice...
January 17, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28089818/notochordal-cell-derived-conditioned-medium-nccm-protects-human-nucleus-pulposus-cells-from-stress-induced-apoptosis
#3
Arne Mehrkens, Ajay Matta, M Zia Karim, Sarah Kim, Michael G Fehlings, Stefan Schaeren, W Mark Erwin
BACKGROUND CONTEXT: Degenerative Disc Disease (DDD) remains without an effective therapy and presents a costly burden to society. PURPOSE: Based upon prior reports concerning the effects of notochordal cell conditioned medium (NCCM) upon disc cells, we performed a proof of principle study to determine whether NCCM could reduce cytotoxic stress induced apoptosis in human disc nucleus pulpous (NP) cells. STUDY DESIGN/SETTING: 'in vitro' fundamental/basic science study METHODS: NP cells derived from 15 patients undergoing spinal surgery were treated with interleukin 1β (IL-1β) and Fas ligand (FasL) or etoposide in the presence of NCCM...
January 9, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28078594/exploration-of-fas-s-nitrosylation-by-the-biotin-switch-assay
#4
Ali Bettaieb, Catherine Paul, Stéphanie Plenchette
S-nitrosylation is the covalent attachment of nitric oxide radical to the thiol side chain of cysteine. The death receptor Fas/CD95 can be S-nitrosylated in cancer cell lines by NO donors or iNOS activation. This posttranslational modification (PTM) induces Fas aggregation into lipid rafts and enhances FasL-mediated signaling and apoptosis. In this report, we describe the detection of Fas S-nitrosylation by the most commonly used method, the biotin switch assay (BSA) technique, that allows the detection of this very labile covalent modification in cells or tissues...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28078577/production-of-the-non-apoptotic-metalloprotease-cleaved-cd95l-and-its-cytotoxic-recombinant-counterpart-designed-ig-cd95l
#5
Eléonore Lepvrier, Sophie Martin, Brigitte Collet
The ligand of CD95, CD95L (also known as FasL or CD178), is a type II transmembrane protein that belongs to the Tumor Necrosis factor (TNF) family (Fig. 1a). This membrane-bound cytokine is mainly expressed at the surface of activated T lymphocytes and natural killer cells, where it is used as an apoptotic factor to eliminate infected and transformed cells (Strasser et al., Immunity 30:180-192, 2009).
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28078537/sirt2-mediated-foxo3a-deacetylation-drives-its-nuclear-translocation-triggering-fasl-induced-cell-apoptosis-during-renal-ischemia-reperfusion
#6
Yan Wang, Yu Mu, Xiaorui Zhou, Huaixue Ji, Xing Gao, Wen Wen Cai, Qiuhua Guan, Tie Xu
We have found that Fas/FasL-mediated "extrinsic" pathway promoted cell apoptosis induced by renal ischemic injury. This study is to elucidate the upstream mechanism regulating FasL-induced extrinsic pathway during renal ischemia/reperfusion. Results demonstrated that when SIRT2 was activated by renal ischemia/reperfusion, activated SIRT2 could bind to and deacetylate FOXO3a, promoting FOXO3a nuclear translocation which resulted in an increase of nuclear FOXO3a along with FasL expression and activation of caspase8 and caspase3, triggering cell apoptosis during renal ischemia/reperfusion...
January 11, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28063953/pathological-analysis-detection-of-antigens-fasl-expression-analysis-and-leucocytes-survival-analysis-in-tilapia-oreochromis-niloticus-after-infection-with-green-fluorescent-protein-labeled-streptococcus-agalactiae
#7
Jingyuan Wang, Jinying Wu, Liyuan Yi, Zengxin Hou, Wensheng Li
The pathogenesis of Streptococcus agalactiae infection in tilapia has not been fully described. To understand this, we investigated the clinic-pathological features of acute experimental septicemia in tilapia (Oreochromis niloticus) after receiving an intra-peritoneal injection with S. agalactiae THN-1901GFP. Immunohistochemistry and sections of pathological tissues were used to estimate the level of damage in the head-kidney, liver, spleen and trunk-kidney. The expression of FasL was analyzed by western blotting in these samples based on their damage levels...
January 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28027556/overexpression-of-tp53-tp53i3-and-birc5-alterations-of-gene-regulation-of-apoptosis-and-aging-of-human-immune-cells-in-a-remote-period-after-radiation-exposure
#8
I N Ilienko, D A Bazyka
OBJECTIVE: To identify a contributive role of changes in gene regulation of apoptosis and telomere length at tran scriptional and translational levels to the formation of radiation induced effects in immune system. PATIENTS AND METHODS: Study groups included 310 Chornobyl (Chornobyl) cleanup workers (dose of external expo sure (360.82 ± 32.3) mSv; age 58.9 ± 0.6 (M ± SD) years) and control (n = 77; age (52.9 ± 0.64) (M ± SD) years). Expression of CD95, phosphatidylserine receptors, bcl2 and p53 proteins was studied by flow cytometry; the relative expression (RQ) of BAX, BIRC5, FASLG, MADD, MAPK14, TP53, TP53I3, TERT, TERF1, TERF2 genes was performed using 7900 HT Fast RT PCR System and TagMan technology...
December 2016: Problemy Radiat︠s︡iĭnoï Medyt︠s︡yny Ta Radiobiolohiï
https://www.readbyqxmd.com/read/28008595/defective-expression-of-apoptosis-related-molecules-in-multiple-sclerosis-patients-is-normalized-early-after-autologous-haematopoietic-stem-cell-transplantation
#9
G L V de Oliveira, A F Ferreira, E P L Gasparotto, S Kashima, D T Covas, C T Guerreiro, D G Brum, A A Barreira, J C Voltarelli, B P Simões, M C Oliveira, F A de Castro, K C R Malmegrim
Defective apoptosis might be involved in the pathogenesis of multiple sclerosis (MS). We evaluated apoptosis-related molecules in MS patients before and after autologous haematopoietic stem cell transplantation (AHSCT) using BCNU, Etoposide, AraC and Melphalan (BEAM) or cyclophosphamide (CY)-based conditioning regimens. Patients were followed for clinical and immunological parameters for 2 years after AHSCT. At baseline, MS patients had decreased proapoptotic BAD, BAX and FASL and increased A1 gene expression when compared with healthy counterparts...
November 7, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28002605/knockdown-of-the-putative-lifeguard-homologue-cg3814-in-neurons-of-drosophila-melanogaster
#10
P G M'Angale, B E Staveley
Lifeguard is an integral transmembrane protein that modulates FasL-mediated apoptosis by interfering with the activation of caspase 8. It is evolutionarily conserved, with homologues present in plants, nematodes, zebra fish, frog, chicken, mouse, monkey, and human. The Lifeguard homologue in Drosophila, CG3814, contains the Bax inhibitor-1 family motif of unknown function. Downregulation of Lifeguard disrupts cellular homeostasis and disease by sensitizing neurons to FasL-mediated apoptosis. We used bioinformatic analyses to identify CG3814, a putative homologue of Lifeguard, and knocked down CG3814/LFG expression under the control of the Dopa decarboxylase (Ddc-Gal4) transgene in Drosophila melanogaster neurons to investigate whether it possesses neuroprotective activity...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28002510/caspase-3-expression-and-plasma-level-of-fas-ligand-as-apoptosis-biomarkers-in-inflammatory-endotoxemic-lung-injury
#11
Raluca Ştefania Fodor, Anca Meda Georgescu, Bianca Liana Grigorescu, Adrian Dan Cioc, Mihaly Veres, Ovidiu Simion Cotoi, Pal Fodor, Sanda Maria Copotoiu, Leonard Azamfirei
OBJECTIVE: The objective of this study is to evaluate if the immunohistochemical expression of a pulmonary apoptosis marker and plasma level of Fas ligand (FasL) correlates with the dose- and time-dependent severity of lung injury, induced by the administration of lipo-polysaccharide (LPS) in an endotoxemic rat model. MATERIALS AND METHODS: Our study included 30 male Wistar rats, randomly divided into three groups: one control group (n=6) and two experimental groups (n=12÷group), in whom we induced endotoxemia by intraperitoneal injection of progressively increasing doses of LPS (5, 10 mg÷kg)...
2016: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/27989714/ifny-and-ifny-treg-subsets-with-stable-and-unstable-foxp3-expression-in-kidney-transplant-recipients-with-good-long-term-graft-function
#12
Karina Trojan, Christian Unterrainer, Mostafa Aly, Li Zhu, Rolf Weimer, Nuray Bulut, Christian Morath, Gerhard Opelz, Volker Daniel
BACKGROUND: Treg are a heterogenous cell population. In the present study we attempted to identify Treg subsets that might contribute to stable and good long-term graft function. METHOD: Lymphocyte and Treg subsets were studied in 136 kidney transplant recipients with good long-term graft function and in 52 healthy control individuals using eight-color-fluorescence flow cytometry. Foxp3 TSDR methylation status was investigated in enriched IFNy+ and IFNy- Treg preparations using high resolution melt analysis...
October 29, 2016: Transplant Immunology
https://www.readbyqxmd.com/read/27981746/galectin-3-promotes-caspase-independent-cell-death-of-hiv-1-infected-macrophages
#13
Jing Xue, Chunyan Fu, Zhe Cong, Lingjuan Peng, Zhuoying Peng, Ting Chen, Wei Wang, Hong Jiang, Qiang Wei, Chuan Qin
HIV-1-infected macrophages are a key contributor to the formation of a viral reservoir and new treatment strategies focus on eliminating this pool of virus. Galectin-3 is a potent apoptosis-inducing protein that regulates diverse cellular activities. In the present study, we investigated whether galectin-3 could induce cell death in HIV-1-infected macrophages using HIV-1-infected THP1 monocytes (THP1-MNs) and THP1-derived macrophages (THP1-MΦs) as in vitro cellular models. We found that THP1-MΦs were more resistant than the THP1-MNs to HIV-1 infection-induced death, and that HIV-1 infection of the THP1-MΦs increased expression of the anti-apoptotic proteins Mcl-1, Bcl-2 and Bcl-xL...
November 7, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27977511/rituximab-response-in-follicular-lymphoma-is-associated-with-the-rs20575-polymorphism-in-trailr1-extrinsic-apoptosis-trigger
#14
Rocío Gutiérrez-Cívicos, Ana M Hurtado, Daniel Torres-Moreno, José J Sanchez-Blanco, Ignacio Español, Luciano Consuegra-Sánchez, Elena Perez-Ceballos, María D Gutiérrez-Meca, Andrés Jerez, Pablo Conesa-Zamora
OBJECTIVE: Rituximab in combination with chemotherapy has been proven to increase progression-free and overall survival in follicular lymphoma (FL), but there is considerable interindividual variability in the response. Extrinsic pathway apoptosis triggered by death receptors seems to be involved in the mechanism of action of monoclonal antibodies. This study aimed to assess the association between TRAILR1/TRAIL polymorphisms (rs20575, rs20576, rs2230229, rs12488654) and rituximab response and the relationship with FASL rs763110, previously found to be associated with rituximab response...
December 13, 2016: Pharmacogenetics and Genomics
https://www.readbyqxmd.com/read/27941708/chloral-hydrate-treatment-induced-apoptosis-of-macrophages-via-fas-signaling-pathway
#15
Jun Cai, Yanxia Peng, Ting Chen, Huanjin Liao, Lifang Zhang, Qiuhua Chen, Yiming He, Ping Wu, Tong Xie, Qingjun Pan
BACKGROUND There are recent reports on several anesthetics that have anti-inflammatory and anti-infective effects apart from their uses for pain relief and muscle relaxation. Chloral hydrate is a clinical anesthetic drug and sedative that has also been reported to attenuate inflammatory response, but the mechanisms are not clearly understood. MATERIAL AND METHODS This study investigated the effect of chloral hydrate treatment on the apoptosis of macrophages and explored the underlying mechanisms. RAW264.7 macrophages were treated with various concentrations of chloral hydrate for various lengths of time...
December 10, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27935860/t-lymphocyte-shp2-deficiency-triggers-anti-tumor-immunity-to-inhibit-colitis-associated-cancer-in-mice
#16
Wen Liu, Wenjie Guo, Lihong Shen, Zhen Chen, Qiong Luo, Xiaolin Luo, Gen-Sheng Feng, Yongqian Shu, Yanhong Gu, Qiang Xu, Yang Sun
Nonresolving inflammation is involved in the initiation and progression process of tumorigenesis. Src homology 2 domain-containing tyrosine phosphatase 2 (SHP2) is known to inhibit acute inflammation but its role in chronic inflammation-associated cancer remains unclear. The role of SHP2 in T cells in dextran sulfate sodium (DSS)-induced colitis and azoxymethane-DSS-induced colitis-associated carcinogenesis was examined using SHP2CD4-/- conditional knockout mice. SHP2 deficiency in T cells aggravated colitis with increased level of pro-inflammatory cytokines including IFN-γ and IL-17A...
December 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27932443/pd-l1-blockade-enhances-response-of-pancreatic-ductal-adenocarcinoma-to-radiotherapy
#17
Abul Azad, Su Yin Lim, Zenobia D'Costa, Keaton Jones, Angela Diana, Owen J Sansom, Philipp Kruger, Stanley Liu, W Gillies McKenna, Omer Dushek, Ruth J Muschel, Emmanouil Fokas
Pancreatic ductal adenocarcinoma (PDAC) is considered a non-immunogenic tumor, and immune checkpoint inhibitor monotherapy lacks efficacy in this disease. Radiotherapy (RT) can stimulate the immune system. Here, we show that treatment of KPC and Pan02 murine PDAC cells with RT and gemcitabine upregulated PD-L1 expression in a JAK/Stat1-dependent manner. In vitro, PD-L1 inhibition did not alter radio- and chemosensitivity. In vivo, addition of anti-PD-L1 to high (12, 5 × 3, 20 Gy) but not low (6, 5 × 2 Gy) RT doses significantly improved tumor response in KPC and Pan02 allografts...
December 8, 2016: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/27919962/ascorbyl-stearate-promotes-apoptosis-through-intrinsic-mitochondrial-pathway-in-hela-cancer-cells
#18
Shirish D Mane, Maikho Thoh, Deepak Sharma, Santosh K Sandur, K Akhilender Naidu
BACKGROUND: Ascorbic acid is proposed to have antitumor potential against certain cancer types but has the limitation of requiring high doses for treating cancer. Ascorbyl stearate (ASC-S) is a fatty acid ester derivative of ascorbic acid with comparable potent apoptotic activity. The present study was aimed at understanding the pathway involved in apoptotic activity of ASC-S in cervical cancer cells. MATERIALS AND METHODS: The effect of ASC-S on reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) was studied in HeLa cells...
2016: Anticancer Research
https://www.readbyqxmd.com/read/27910206/balance-between-il-3-and-type-iinterferons-and-their-interrelationship-with-fasl-dictates-lifespan-and-effector-functions-of-human-basophils
#19
B R Hagmann, A Odermatt, T Kaufmann, C A Dahinden, M Fux
BACKGROUND: In contrast to eosinophils and neutrophils, the regulation of the lifespan of human basophils is poorly defined, with the exception of the potent anti-apoptotic effect of IL-3 that also promotes pro-inflammatory effector functions and phenotypic changes. Type I IFNs (IFN-α, IFN-β), which are well known for their anti-viral activities, have the capacity to inhibit allergic inflammation. OBJECTIVE: To elucidate whether type I IFNs have the potential to abrogate the lifespan and/or effector functions of human basophils...
January 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27909160/evaluation-of-polymorphic-variants-in-apoptotic-genes-and-their-role-in-susceptibility-and-clinical-progression-to-systemic-lupus-erythematosus
#20
N Glesse, P Vianna, L M G Paim, M C C Matte, A K K Aguiar, P L Palhano, O A Monticielo, C V Brenol, R M Xavier, J A B Chies
BACKGROUND: Systemic lupus erythematosus (SLE) is an autoimmune disease marked by the disruption of the immune homeostasis. Patients exhibit a wide range of clinical manifestations, and environmental and genetic factors are involved in SLE pathogenesis. Evidence suggests that abnormalities in the cellular and molecular events that coordinate apoptosis may favour the generation of autoantigens involved in autoimmunity. In this way, the apoptotic deregulation may be affected by polymorphic variants in apoptotic-related genes...
November 30, 2016: Lupus
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