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https://www.readbyqxmd.com/read/29344176/effect-of-fraxetin-on-proliferation-and-apoptosis-in-breast-cancer-cells
#1
Guodong Liu, Zhenfang Liu, Yuexiang Yan, Haiyan Wang
The aim of the present study was to examine the effect of fraxetin on proliferation and apoptosis in the MCF-7 breast cancer cell line. Cell proliferation was measused using an MTT assay and 4',6-diamidino-2-phenylindole (DAPI) staining was used to determine shrinkage and condensation. RT-PCR was used to examine the expression of factor-associated suicide (Fas) and Fas ligand (FasL) mRNA, and western blot analysis was used to examine Bax and Bcl-2 protein. MTT showed that the proliferation of MCF-7 cells was significantly inhibited by fraxetin in a dose-dependent manner...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343518/fibroblast-specific-%C3%AE-catenin-signaling-dictates-the-outcome-of-aki
#2
Dong Zhou, Haiyan Fu, Liangxiang Xiao, Hongyan Mo, Hui Zhuo, Xiaojun Tian, Lin Lin, Jianhua Xing, Youhua Liu
AKI is a devastating condition with high morbidity and mortality. The pathologic features of AKI are characterized by tubular injury, inflammation, and vascular impairment. Whether fibroblasts in the renal interstitium have a role in the pathogenesis of AKI is unknown. In this study, we investigated the role of fibroblast-specific β-catenin signaling in dictating the outcome of AKI, using conditional knockout mice in which β-catenin was specifically ablated in fibroblasts (Gli1-β-cat-/-). After ischemia-reperfusion injury (IRI), Gli1-β-cat-/- mice had lower serum creatinine levels and less morphologic injury than Gli1-β-cat+/+ littermate controls...
January 17, 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29339218/protective-effect-of-patchoulene-epoxide-against-ethanol-induced-gastric-ulcer-in-rats-influence-on-oxidative-stress-inflammation-and-apoptosis
#3
Jiali Liang, Yaoxing Dou, Xue Wu, Huilin Li, Jiazhen Wu, Qionghui Huang, Dandan Luo, Tiegang Yi, Yuhong Liu, Ziren Su, Jianping Chen
Patchoulene epoxide (PAO), a tricyclic sesquiterpene isolated from the long-strored patchouli oil, has been demonstrated the anti-inflammatory activity in vivo based on our previous study. However, the gastroprotective effect of PAO still remains unknown. Therefore, in the present study, ethanol-induced gastric ulcer in rats was carried out to evaluate the anti-ulcerogenic activity of PAO and to elucidate the potential mechanisms that involved. According to our results, macroscopic examination revealed that pre-treatment with PAO signficantly reduced ethanol-induced gastric ulcer areas as compared with the vehicle group, which was also supported by histological evaluation...
January 12, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29339045/deficiency-of-dietary-pyridoxine-disturbed-the-intestinal-physical-barrier-function-of-young-grass-carp-ctenopharyngodon-idella
#4
Pei Wu, Xin Zheng, Xiao-Qiu Zhou, Wei-Dan Jiang, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Yong-An Zhang, Lin Feng
The aim of this study was to assess the effects of dietary pyridoxine (PN) deficiency on intestinal antioxidant capacity, cell apoptosis and intercellular tight junction in young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (231.85 ± 0.63 g) were fed six diets containing graded levels of PN (0.12-7.48 mg/kg diet) for 10 weeks. At the end of the feeding trial, the fish were challenged with Aeromonas hydrophila for 2 weeks. The results showed that compared with the optimal PN level, PN deficiency (1) increased the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC), decreased the activities and mRNA levels of antioxidant enzymes such as copper, zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) (P < ...
January 12, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29338314/inflammatory-cytokines-in-the-papillary-tips-and-urine-of-nephrolithiasis-patients
#5
Andrew Yang Sun, Bryan Hinck, Benjamin R Cohen, Karen Keslar, Robert L Fairchild, Manoj Monga
INTRODUCTION: Intrarenal inflammation has been implicated in the pathogenesis of nephrolithiasis, with prior work showing increased urine levels of IL-6, IL-8, and CCL-2 in stone patients. However, no studies have assessed for inflammation in the renal papillae. We sought to characterize novel papillary tip and urinary biomarkers in stone patients. METHODS: 92 patients with nephrolithiasis undergoing percutaneous nephrolithotomy were enrolled. Papillary tip biopsies, kidney urine, and bladder urine were collected, as well as voided urine from 8 healthy volunteers...
January 16, 2018: Journal of Endourology
https://www.readbyqxmd.com/read/29324390/nfat-1-hyper-activation-by-methionine-enkephalin-menk-significantly-induces-cell-apoptosis-of-rats-c6-glioma-in-vivo-and-in-vitro
#6
Wei-Cheng Lu, Hui Xie, Xin-Xin Tie, Ruizhe Wang, An-Hua Wu, Feng-Ping Shan
The aim of the work was to investigate the effect and possible mechanism of MENK on the growth of rat C6 glioma in vivo or in vitro. Our findings showed that MENK could inhibit the growth of rat C6 glioma, prolong median survival times in tumor-bearing rats, and induce glioma cell apoptosis. Moreover, MENK could increase the activities of caspase-3, caspase-8 and caspase-9. It also increased the expression of Fas, FasL, Bax, while decreased the expression of Bcl-2. We further confirmed that MENK could increase opioid receptors MOR and DOR expressions, Ca2+ influx into the cytoplasm, and a substantial increase of NFAT1accumulation in the nuclei in C6 glioma cell...
January 8, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29312612/the-anti-cancer-effects-and-mechanisms-of-scutellaria-barbata-d-don-on-cl1-5-lung-cancer-cells
#7
Chin-Chuan Chen, Chun-Pin Kao, Mei-Miao Chiu, Shu-Huei Wang
Lung cancer, with a poor prognosis and resistance to chemotherapy, is the most common malignant tumor and has the highest mortality rate worldwide. Scutellaria barbata D. Don (SB), which is derived from the dried whole plant of Labiatae, is a well-known anti-inflammatory and anti-cancer herb. The aim of this study was to examine the anti-cancer effects and precise regulatory mechanisms of SB in CL1-5 lung cancer cells. In an in vitro assay, we found that the anti-tumor mechanism of SB was due to P38/SIRT1-regulated cell apoptosis through G2/M phase arrest and ER stress-, intrinsic mitochondrial-, and extrinsic FAS/FASL-mediated pathways...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29310106/vaccination-with-fasl-tcl-plus-mhsp65-induces-improved-anti-lung-cancer-immunity-in-mice
#8
Bohan Dong, Guangli Dai, Yuanyuan Ding, Beiru Wang, Siyuan Zhang
In a previous study, we constructed a MHSP65-TCL anti-lung cancer vaccine with Lewis lung carcinoma TCL plus MHSP65, and illustrated its anti-lung cancer effect through specific and nonspecific anti-tumor immunity. However, TCL contains some immunoinhibit components such as FasL. If this component can be eliminated from TCL, the anti-tumor immunity of MHSP65-TCL constructed with TCL should be improved. In the present study, we knocked down FasL from Lewis lung carcinoma cells and prepared MHSP65-(FasL-/TCL) with this cell line's TCL...
January 5, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29307546/the-3-methyl-4-nitrophenol-pnmc-compromises-airway-epithelial-barrier-function
#9
Yun-Fang An, Xiao-Rui Geng, Li-Hua Mo, Jiang-Qi Liu, Li-Teng Yang, Xiao-Wen Zhang, Zhi-Gang Liu, Chang-Qing Zhao, Ping-Chang Yang
BACKGROUND AND AIMS: It is recognized that the air pollution is associated with the pathogenesis of airway diseases. This study aims to elucidate the role of the 3-methyl-4-nitrophenol (PNMC), one of the components of diesel-exhaust particles, in compromising the airway epithelial barrier integrity. METHODS: A549 cells, an airway epithelial cell line, were cultured to monolayers to be used as an in vitro epithelial barrier model. BALB/c mice were treated with nasal drops containing PNMC to test the effects of PNMC on alternating the airway epithelial barrier functions...
January 4, 2018: Toxicology
https://www.readbyqxmd.com/read/29301308/msp-4-an-antimicrobial-peptide-induces-apoptosis-via-activation-of-extrinsic-fas-fasl-and-intrinsic-mitochondria-mediated-pathways-in-one-osteosarcoma-cell-line
#10
Hsiao-Mei Kuo, Chung-Chih Tseng, Nan-Fu Chen, Ming-Hong Tai, Han-Chun Hung, Chien-Wei Feng, Shu-Yu Cheng, Shi-Ying Huang, Yen-Hsuan Jean, Zhi-Hong Wen
Osteosarcoma (OS) is a common malignant bone cancer. The relatively high density of a person's bone structure means low permeability for drugs, and so finding drugs that can be more effective is important and should not be delayed. MSPs are marine antimicrobial peptides (AMP) and natural compounds extracted from Nile tilapia (Oreochromis niloticus). MSP-4 is a part of the AMPs series, with the advantage of having a molecular weight of about 2.7-kDa and anticancer effects, although the responsible anticancer mechanism is not very clear...
January 2, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29299971/the-role-of-the-fas-fasl-signaling-pathway-in-environmental-toxicant-induced-testicular-cell-apoptosis-an-update
#11
Mei Wang, Ping Su
The Fas/FasL signaling pathway is one of the major pathways that regulate apoptosis. Increasing studies have shown that the activation of the Fas/FasL signaling pathway is closely associated with testicular cell apoptosis. However, the mechanism involved is still unclear. We discuss recent findings regarding the molecular mechanisms by which environmental toxicants induce testicular pathology via Fas/FasL signaling. These findings suggest that Fas/FasL signaling is employed to impact the sensitivity (a response to external factors) of germ cells, disrupt steroidogenic hormone and cytokine metabolism mediated by Sertoli cells, and elicit the activation of NFAT (nuclear factor of activated T-cells) in Leydig cell apoptosis...
January 4, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29285062/effects-of-apigenin-on-the-expression-levels-of-b-cell-lymphoma-2-fas-and-fas-ligand-in-renal-ischemia-reperfusion-injury-in-rats
#12
Yang Liu, Xiuheng Liu, Lei Wang, Yang Du, Zhiyuan Chen, Hui Chen, Jia Guo, Xiaodong Weng, Xiao Wang, Ming Wang, Zhishun Wang
The aim of the present study was to investigate the effect and possible mechanism of apigenin on renal ischemia-reperfusion (I/R) injury in rats, as well as in in vitro experiments. In total, 36 rats were subjected to 45 min of renal ischemia, with or without treatment prior to ischemia with different concentrations of apigenin (2, 10 and 50 mg/kg) administered intravenously. All rats were sacrificed at 24 h after I/R injury. The serum creatinine (Cr) and blood urea nitrogen (BUN) levels were analyzed, and histological examination was conducted...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29259391/melatonin-partially-protects-661w-cells-from-h2o2-induced-death-by-inhibiting-fas-fasl-caspase-3
#13
Aída Sánchez-Bretaño, Kenkichi Baba, Uzair Janjua, Ilaria Piano, Claudia Gargini, Gianluca Tosini
Purpose: Previous studies have shown that melatonin (MEL) signaling is involved in the modulation of photoreceptor viability during aging. Recent work by our laboratory suggested that MEL may protect cones by modulating the Fas/FasL-caspase-3 pathway. In this study, we first investigated the presence of MEL receptors (MT1 and MT2) in 661W cells, then whether MEL can prevent H2O2-induced cell death, and last, through which pathway MEL confers protection. Methods: The mRNA and proteins of the MEL receptors were detected with quantitative PCR (q-PCR) and immunocytochemistry, respectively...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29251002/mannosylated-t-tn-with-freund-s-adjuvant-induces-cellular-immunity
#14
Hye-Youn Son, Vasso Apostolopoulos, Chul-Woo Kim
Inducing cancer-specific cellular immune responses has become an attractive strategy in cancer treatment. In this study, we investigated the role of several adjuvants in eliciting T/Tn-specific cellular immunity and protection against T/Tn expressing tumor challenge. T/Tn (9:1) antigen was purified from blood type "O" erythrocytes donated from healthy Korean volunteers. Immunization was performed using: T/Tn only, T/Tn mixed with Freund's adjuvant (T/Tn + FA), keyhole limpet hemocyanin (KLH)-conjugated T/Tn mixed with FA (KLH-T/Tn + FA), and oxidized mannan-conjugated T/Tn mixed with FA (ox-M-T/Tn + FA)...
December 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/29245921/aflatoxin-b1-affects-apoptosis-and-expression-of-death-receptor-and-endoplasmic-reticulum-molecules-in-chicken-spleen
#15
Panpan Zhu, Zhicai Zuo, Zhixiang Zheng, Fengyuan Wang, Xi Peng, Jing Fang, Hengmin Cui, Caixia Gao, Hetao Song, Yi Zhou, Xici Liu
Aflatoxin B1 (AFB1) is a natural product of the Aspergillus genus of molds, which grow on several foodstuffs stored in hot moist conditions, and is among the most potent hepatocarcinogens and immunosuppression presently known. The latter was related to the up-regulated apoptosis of immune organs. However, the effect of expression of death receptor and endoplasmic reticulum molecules in AFB1-induced apoptosis of chicken splenocytes was largely unknown. The objective of this study was to investigate this unknown field...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29223855/increased-adam10-expression-in-patients-with-immune-thrombocytopenia
#16
Jianlin Qiao, Qi Luo, Na Liu, Guangyu Wei, Xiaoqing Wu, Jun Lu, Kai Tang, Yulu Wu, Jie Zi, Xiaoqian Li, Yun Liu, Wen Ju, Kunming Qi, Zhiling Yan, Zhenyu Li, Lingyu Zeng, Kailin Xu
Immune thrombocytopenia (ITP) is an autoimmune disease, which is characterized by abnormal of T immunity. A disintegrin and metalloproteinase (ADAM) 10, a member of proteinase family, has been demonstrated to regulate T cell proliferation and effector function. Considering the closely association of dysregulation of T cell function with ITP, whether ADAM10 involves in the pathogenesis of ITP remains unclear. In this study, 54 active ITP patients, 18 ITP in remission and 24 age and gender matched healthy control were enrolled...
December 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29222028/sodium-butyrate-enhanced-physical-barrier-function-referring-to-nrf2-jnk-and-mlck-signaling-pathways-in-the-intestine-of-young-grass-carp-ctenopharyngodon-idella
#17
Pei Wu, Li Tian, Xiao-Qiu Zhou, Wei-Dan Jiang, Yang Liu, Jun Jiang, Fei Xie, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Juan Yang, Yong-An Zhang, He-Qun Shi, Lin Feng
This study evaluated the effect of dietary sodium butyrate (SB) supplementation on the intestinal physical barrier function of young grass carp (Ctenopharyngodon idella). The fish were fed one powdery sodium butyrate (PSB) diet (1000.0 mg kg-1 diet) and five graded levels of microencapsulated sodium butyrate (MSB) diets: 0.0 (control), 500.0, 1000.0, 1500.0 and 2000.0 mg kg-1 diet for 60 days. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila to explore the effect of SB supplementation on intestinal physical barrier function and the potential mechanisms in fish...
December 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29218706/genome-wide-rna-seq-identifies-fas-fasl-mediated-tumoricidal-activity-of-embryonic-stem-cells
#18
Yatong Li, Yongna Fan, Jing Xu, Peng Zhang, Taijiao Jiang, Menghua Dai, Limin Li
The discovery of tumor tropism of stem cells revealed the intimate relationship between stem cells and tumor cells, but the functional role of stem cells in tumorigenesis is poorly understood. To investigate embryonic stem cell (ESC) and tumor cell interactions, we co-cultured mouse ESCs with mouse melanoma B16-F10 cells or mouse pancreatic tumor Pan02 cells, and found that ESCs significantly inhibited tumor cell proliferation. Coculture of ESCs and tumor cells resulted in significant inhibition of tumorigenesis in vivo...
December 8, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29216642/taurine-protects-mouse-spermatocytes-from-ionizing-radiation-induced-damage-through-activation-of-nrf2-ho-1-signaling
#19
Wenjun Yang, Jinfeng Huang, Bang Xiao, Yan Liu, Yiqing Zhu, Fang Wang, Shuhan Sun
BACKGROUND/AIMS: The increasing prevalence of ionizing radiation exposure has inevitably raised public concern over the potential detrimental effects of ionizing radiation on male reproductive system function. The detection of drug candidates to prevent reproductive system from damage caused by ionizing radiation is urgent. We aimed to investigate the protective role of taurine on the injury of mouse spermatocyte-derived cells (GC-2) subjected to ionizing radiation. METHODS: mouse spermatocytes (GC-2 cells) were exposed to ionizing radiation with or without treatment of Taurine...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29211704/histone-deacetylase-hdac-inhibitor-suberoylanilide-hydroxamic-acid-saha-induces-apoptosis-in-prostate-cancer-cell-lines-via-the-akt-foxo3a-signaling-pathway
#20
Xuan-Yan Shi, Wei Ding, Tie-Qiu Li, Yi-Xiong Zhang, Shan-Chao Zhao
BACKGROUND Histone deacetylase (HDAC) inhibitors are emerging as a new class of anti-cancer drugs that promote cancer cell apoptosis, and include suberoylanilide hydroxamic acid (SAHA). The aim of this study was to investigate the mechanism of SAHA-induced apoptosis in human prostate cancer cell lines, DU145 and PC-3. MATERIAL AND METHODS Cell lines, DU145 and PC-3, were studied before and after treatment with SAHA. The effects of SAHA treatment on cell proliferation were studied using the MTT cell proliferation assay...
December 6, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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