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https://www.readbyqxmd.com/read/29168730/comparative-proteomics-analyses-of-pollination-response-in-endangered-orchid-species-dendrobium-chrysanthum
#1
Wei Wang, Hongyang Yu, Tinghai Li, Lexing Li, Guoqiang Zhang, Zhongjian Liu, Tengbo Huang, Yongxia Zhang
Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual reproduction in D. chrysanthum relies on the compatibility of pollination. To provide a better understanding of the mechanism of pollination, the differentially expressed proteins (DEP) between the self-pollination (SP) and cross-pollination (CP) pistil of D...
November 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29165806/host-preference-and-network-properties-in-biotrophic-plant-fungal-associations
#2
Sergei Põlme, Mohammad Bahram, Hans Jacquemyn, Peter Kennedy, Petr Kohout, Mari Moora, Jane Oja, Maarja Öpik, Lorenzo Pecoraro, Leho Tedersoo
Analytical methods can offer insights into the structure of biological networks, but mechanisms that determine the structure of these networks remain unclear. We conducted a synthesis based on 111 previously published datasets to assess a range of ecological and evolutionary mechanisms that may influence the plant-associated fungal interaction networks. We calculated the relative host effect on fungal community composition and compared nestedness and modularity among different mycorrhizal types and endophytic fungal guilds...
November 22, 2017: New Phytologist
https://www.readbyqxmd.com/read/29165576/timing-of-first-respiratory-virus-detections-in-infants-a-community-based-birth-cohort-study
#3
Mohinder Sarna, Robert S Ware, Stephen B Lambert, Theo P Sloots, Michael D Nissen, Keith Grimwood
Background: Determining timing of first virus detection episodes (fVDEs) for different respiratory viruses in infants identifies risk periods and informs preventive interventions, including vaccination. We describe the ages and nature of fVDEs in an infant birth cohort, and explore factors associated with increased odds of symptomatic fVDEs. Methods: The Observational Research in Childhood Infectious Diseases (ORChID) study is a community-based birth cohort describing acute respiratory infections in infants until their second birthday...
November 17, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29163591/transcriptomic-and-hormonal-analyses-reveal-that-yuc-mediated-auxin-biogenesis-is-involved-in-shoot-regeneration-from-rhizome-in-cymbidium
#4
Yang Liu, Hai-Liang Zhang, He-Rong Guo, Li Xie, Rui-Zhen Zeng, Xiang-Qian Zhang, Zhi-Sheng Zhang
Cymbidium, one of the most important orchid genera in horticulture, can be classified into epiphytic and terrestrial species. Generally, epiphytic Cymbidium seedlings can be easily propagated by tissue culture, but terrestrial seedlings are difficult to propagate. To date, the molecular mechanisms underlying the differences in the ease with which terrestrial and epiphytic cymbidiums can be propagated are largely unknown. Using RNA-sequencing, quantitative reverse transcription PCR and enzyme-linked immunosorbent assay, Cymbidium 'Xiaofeng' (CXF), which can be efficiently micropropagated, and terrestrial Cymbidium sinense 'Qijianbaimo' (CSQ), which has a low regeneration ability, were used to explore the molecular mechanisms underlying the micropropagation ability of Cymbidium species...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29159723/anatomical-and-diffusional-determinants-inside-leaves-explain-the-difference-in-photosynthetic-capacity-between-cypripedium-and-paphiopedilum-orchidaceae
#5
Zhong-Hui Yang, Wei Huang, Qiu-Yun Yang, Wei Chang, Shi-Bao Zhang
Comparing with other angiosperms, most members within the family Orchidaceae have lower photosynthetic capacities. However, the underlying mechanisms remain unclear. Cypripedium and Paphiopedilum are closely related phylogenetically in Orchidaceae, but their photosynthetic performances are different. We explored the roles of internal anatomy and diffusional conductance in determining photosynthesis in three Cypripedium and three Paphiopedilum species, and quantitatively analyzed their diffusional and biochemical limitations to photosynthesis...
November 20, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/29158642/insights-into-nuclear-dna-content-hydrogen-peroxide-and-antioxidative-enzyme-activities-during-transverse-thin-cell-layer-organogenesis-and-ex-vitro-acclimatization-of-malaxis-wallichii-a-threatened-medicinal-orchid
#6
Biswajit Bose, Suman Kumaria, Hiranjit Choudhury, Pramod Tandon
Malaxis wallichii (Lindl.) Deb, a small, perennial, monopodial, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000 m asl. The pseudobulbs are important ingredients of century old drug 'Ashtavarga' and a polyherbal energetic tonic 'Chyavanprash'. An efficient genetically stable in vitro propagation protocol using transverse thin cell layer culture system was established for M. wallichii. In the present report, meta-topolin alone proved to be three times more beneficial compared to other routinely used cytokinins in inducing highest number of shoot buds, plant height and growth of regenerated shoots...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29156555/identification-of-major-flavone-c-glycosides-and-their-optimized-extraction-from-cymbidium-kanran-using-deep-eutectic-solvents
#7
Kyung Min Jeong, Misuk Yang, Yan Jin, Eun Mi Kim, Jaeyoung Ko, Jeongmi Lee
Cymbidium kanran, an orchid exclusively distributed in Northeast Asia, has been highly valued as a decorative plant and traditional herbal medicine. Here, C. kanran extracts were prepared in 70% aqueous methanol using ultrasound-assisted extraction (UAE) and subjected to liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry analysis, which were used for quantitative and qualitative analysis, respectively. It was found that the extracts were rich in flavone C-glycosides including vicenin-2, vicenin-3, schaftoside, vitexin, and isovitexin...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29150333/design-synthesis-and-evaluation-of-novel-dihydrostilbene-derivatives-as-potential-anti-melanogenic-skin-protecting-agents
#8
Yu Zhu, Wen-Hui Pan, Chuen Fai Ku, Hong-Jie Zhang, Siu Wai Tsang
The stems of Dendrobium orchids (Orchidaceae), also known as Shi Hu, have been used for medicinal purposes for centuries in oriental countries. In fact, the health benefits of Shi Hu have been evidenced by its modern pharmacological actions on conquering oxidative stress in pathological conditions. From the extracts of two commonly used Dendrobium species, we obtained discernible amounts of stilbenoids, explicitly trans-resveratrol (1) and dihydro-resveratrol (2), which are prototypical antioxidants. When applied to cultured melanocytes, these stilbenoids, dihydro-resveratrol (2) in particular, significantly reduced melanin formation via inhibiting tyrosinase activity and expression of tyrosinase-related proteins...
October 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29149283/multivalent-rotavirus-vaccine-and-wild-type-rotavirus-strain-shedding-in-australian-infants-a-birth-cohort-study
#9
Suifang Ye, David M Whiley, Robert S Ware, Carl D Kirkwood, Stephen B Lambert, Keith Grimwood
Background: Rotavirus vaccines have reduced moderate-to-severe gastroenteritis episodes in infants and young children. Nevertheless, knowledge gaps exist concerning rotavirus vaccine shedding and vaccine impact upon mild and asymptomatic wild-type infections. Our primary objective was to investigate vaccine shedding in Australian infants where the multivalent human-bovine reassortant rotavirus vaccine, RotaTeq®, was part of the routine vaccination schedule. Methods: The Observational Research in Childhood Infectious Diseases (ORChID) birth cohort study was conducted in Brisbane, Australia, from September 2010 to October 2014...
November 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29143225/diurnal-regulation-of-the-floral-scent-emission-by-light-and-circadian-rhythm-in-the-phalaenopsis-orchids
#10
Yu-Chen Chuang, Ming-Chi Lee, Ya-Lan Chang, Wen-Huei Chen, Hong-Hwa Chen
BACKGROUND: Phalaenopsis bellina and its closely related species, P. violacea, emit linalool, geraniol and their derivatives as the predominant monoterpenes at the full-bloom stages. Geranyl diphosphate synthase (PbGDPS) is the key enzyme that converts precursors for monoterpene biosynthesis. Besides the monoterpenes being synthesized in concert with floral development stages, we noticed that the scent emission of P. bellina and P. violacea was detected mainly in the daytime. RESULTS: The monoterpenes of P...
November 15, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29143146/screening-transferable-microsatellite-markers-across-genus-phalaenopsis-orchidaceae
#11
Ya-Zhu Ko, Huei-Chuan Shih, Chi-Chu Tsai, Hsing-Hua Ho, Pei-Chun Liao, Yu-Chung Chiang
BACKGROUND: Molecular identification based on microsatellite loci is an important technology to improve the commercial breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite subsp. formosana to further examine the transferability of across 21 Phalaenopsis species. METHODS AND RESULTS: Twenty-eight polymorphic microsatellite markers were obtained using the magnetic bead enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus Phalaenopsis...
November 15, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29140976/independent-degradation-in-genes-of-the-plastid-ndh-gene-family-in-species-of-the-orchid-genus-cymbidium-orchidaceae-epidendroideae
#12
Hyoung Tae Kim, Mark W Chase
In this paper, we compare ndh genes in the plastid genome of many Cymbidium species and three closely related taxa in Orchidaceae looking for evidence of ndh gene degradation. Among the 11 ndh genes, there were frequently large deletions in directly repeated or AT-rich regions. Variation in these degraded ndh genes occurs between individual plants, apparently at population levels in these Cymbidium species. It is likely that ndh gene transfers from the plastome to mitochondrial genome (chondriome) occurred independently in Orchidaceae and that ndh genes in the chondriome were also relatively recently transferred between distantly related species in Orchidaceae...
2017: PloS One
https://www.readbyqxmd.com/read/29136520/characterization-of-myeloid-cell-populations-in-human-testes-collected-after-sex-reassignment-surgery
#13
Rosalie Ponte, Franck P Dupuy, Fadi Brimo, Vikram Mehraj, Pierre Brassard, Maud Belanger, Ekaterina Yurchenko, Mohammad-Ali Jenabian, Nicole F Bernard, Jean-Pierre Routy
The testis has been described in animal models as a site of immune privilege, which protects spermatids against tissue damage during inflammation. Myeloid cells, including macrophages and dendritic cells (DC), are defined as key players in the testicular immune privilege in animal models. However, their distribution and frequency in human testis remain poorly described. To overcome the challenges related to tissue sampling, we obtained testicular tissue from men under hormonal therapy who elected to have sex reassignment surgery (SRS)...
October 14, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29136092/microrpm-a-microrna-prediction-model-based-only-on-plant-small-rna-sequencing-data
#14
Kuan-Chien Tseng, Yi-Fan Chiang-Hsieh, Hsuan Pai, Chi-Nga Chow, Shu-Chuan Lee, Han-Qin Zheng, Po-Li Kuo, Guan-Zhen Li, Yu-Cheng Hung, Na-Sheng Lin, Wen-Chi Chang
Motivation: MicroRNAs (miRNAs) are endogenous non-coding small RNAs (of about 22 nucleotides), which play an important role in the post-transcriptional regulation of gene expression via either mRNA cleavage or translation inhibition. Several machine learning-based approaches have been developed to identify novel miRNAs from next generation sequencing (NGS) data. Typically, precursor/genomic sequences are required as references for most methods. However, the non-availability of genomic sequences is often a limitation in miRNA discovery in non-model plants...
November 9, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29112129/enhanced-agronomic-traits-and-medicinal-constituents-of-autotetraploids-in-anoectochilus-formosanus-hayata-a-top-grade-medicinal-orchid
#15
Hsiao-Hang Chung, Shu-Kai Shi, Bin Huang, Jen-Tsung Chen
This study developed an efficient and reliable system for inducing polyploidy in Anoectochilus formosanus Hayata, a top-grade medicinal orchid. The resulting tetraploid gave a significant enhancement on various agronomic traits, including dry weight, fresh weight, shoot length, root length, leaf width, the size of stoma, and number of chloroplasts per stoma. A reduction of the ratio of length to width was observed in stomata and leaves of the tetraploid, and consequently, an alteration of organ shape was found...
November 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29106441/orchid-a-novel-management-annotation-and-machine-learning-framework-for-analyzing-cancer-mutations
#16
Clinton L Cario, John S Witte
Motivation: As whole-genome tumor sequence and biological annotation datasets grow in size, number, and content, there is an increasing basic science and clinical need for efficient and accurate data management and analysis software. With the emergence of increasingly sophisticated data stores, execution environments, and machine learning algorithms, there is also a need for the integration of functionality across frameworks. Results: We present orchid, a python based software package for the management, annotation, and machine learning of cancer mutations...
November 2, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29102461/effect-of-swarna-jibanti-coelogyne-cristata-lindley-in-alleviation-of-chronic-fatigue-syndrome-in-aged-wistar-rats
#17
Achintya Mitra, Tapas Kumar Sur, Sachhidananda Upadhyay, Dipankar Bhattacharyya, Jayram Hazra
BACKGROUND: Swarna Jibanti scientifically known as Coelogyne cristata Lindley (Orchidaceae), an orchid mentioned in Ayurvedic medicine is used to promote healthy life span. OBJECTIVE: The present work was planned to study the efficacy of hydro-alcoholic extract of pseudobulbs of C.cristata (CCE) to assess its role on chronic fatigue syndrome (CFS) induced behavioural and biochemical changes in aged Wistar rats compared to Panax ginseng (PG), a prototype anti-stress agent...
November 1, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29099062/mutational-biases-and-gc-biased-gene-conversion-affect-gc-content-in-the-plastomes-of-dendrobium-genus
#18
Zhitao Niu, Qingyun Xue, Hui Wang, Xuezhu Xie, Shuying Zhu, Wei Liu, Xiaoyu Ding
The variation of GC content is a key genome feature because it is associated with fundamental elements of genome organization. However, the reason for this variation is still an open question. Different kinds of hypotheses have been proposed to explain the variation of GC content during genome evolution. However, these hypotheses have not been explicitly investigated in whole plastome sequences. Dendrobium is one of the largest genera in the orchid species. Evolutionary studies of the plastomic organization and base composition are limited in this genus...
November 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29096695/floral-micromorphology-and-transcriptome-analyses-of-a-fragrant-vandaceous-orchid-vanda-mimi-palmer-for-its-fragrance-production-sites
#19
Conie Toh, Ab Rahim Mohd-Hairul, Nooraini Mohd Ain, Parameswari Namasivayam, Rusea Go, Nur Ashikin Psyquay Abdullah, Meilina Ong Abdullah, Janna Ong Abdullah
BACKGROUND: Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the flowers. RESULTS: Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites...
November 2, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29080459/acute-toxicity-study-and-anti-nociceptive-activity-of-bauhinia-acuminata-linn-leaf-extracts-in-experimental-animal-models
#20
Ashika V Padgaonkar, Sachin V Suryavanshi, Vaishali Y Londhe, Yogesh A Kulkarni
Bauhinia acuminata commonly known as dwarf white orchid tree is traditionally used to treat acute and chronic pain, skin ailments, cancer, diabetes, throat infections and asthma. As there were no scientific reports on use of Bauhinia acuminata for anti-nociceptive activity, the present study was designed to evaluate possible effects of aqueous and alcoholic extracts in experimentally induced pain in animals. Acute toxicity was carried out as per OECD guideline 423. The anti-nociceptive activity was evaluated in Swiss albino mice by hot plate, acetic acid induced writhing and tail immersion tests at three different dose levels (250, 500 and 1000mg/kg) of aqueous and alcoholic extracts...
October 25, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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