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https://www.readbyqxmd.com/read/27877224/the-role-of-copper-transporter-atp7a-in-platinum-resistance-of-esophageal-squamous-cell-cancer-escc
#1
Zhuang-Hua Li, Rongjie Zheng, Jing-Tang Chen, Jun Jia, Miaozhen Qiu
Purpose: Platinum derivatives, such as cisplatin (DDP), carboplatin and oxaliplatin, are widely used components of modern cancer chemotherapy including esophageal squamous cell cancer (ESCC). However, their roles are limited by the impact of intrinsic/acquired resistance mechanisms on tumor responses. Recent studies have shown that the mammalian copper transporters CTR1, ATP7A and ATP7B are involved in cisplatin-resistance to some cancers. Methods: The cytotoxicities of DDP in different cell lines were determined using the MTT assay...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/27813112/developmental-toxicity-of-auranofin-in-zebrafish-embryos
#2
Xiao-Yan Gao, Kang Li, Ling-Ling Jiang, Ming-Fang He, Cun-Hai Pu, Dongzhou Kang, Jingjing Xie
Auranofin (AF) is used in clinic for the treatment of rheumatoid arthritis, repurposing of AF as an anticancer drug has just finished a phase I/II clinical trial, but the developmental toxicity of AF remains obscure. This study focused on its developmental toxicity by using zebrafish embryos. Zebrafish embryos were exposed to different concentrations (1, 2.5, 5, 10 μm) of AF from 2 h post-fertilization (hpf) to 72 hpf. At 72 hpf, two major developmental defects caused by AF were found, namely severe pericardial edema and hypopigmentation, when embryos were exposed to concentrations higher than 2...
November 4, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27797249/impact-of-the-copper-transporter-protein-1-ctr1-polymorphism-on-adverse-events-among-advanced-nonsmall-cell-lung-cancer-patients-treated-with-a-carboplatin-gemcitabine-regimen
#3
Siriluk Kumpiro, Virote Sriuranpong, Nutthada Areepium
BACKGROUND: Platinum-based regimens are effective treatments for advanced non-small cell lung cancer (NSCLC), but the five-year survival rate is still less than 20%. One possible factor appears to be resistance involving polymorphisms in the CTR1 gene which plays an importance role in accumulation of platinum in the cytoplasm. PURPOSE: To establish both prevalence of CTR1 polymorphism and its impact on treatment related toxicity in Thai advanced NSCLC patients. MATERIALS AND METHODS: Thirty-two advanced NSCLC participants received carboplatin and gemcitabine during January to June 2016 at King Chulalongkorn Memorial Hospital (KCMH) were recruited for analysis of the CTR1 rs12686377 genotype...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27690074/effects-of-octylphenol-and-bisphenol-a-on-the-metal-cation-transporter-channels-of-mouse-placentas
#4
Jae-Hwan Lee, Changhwan Ahn, Hee Young Kang, Eui-Ju Hong, Sang-Hwan Hyun, Kyung-Chul Choi, Eui-Bae Jeung
Octylphenol (OP) and bisphenol A (BPA) are known as endocrine-disrupting chemicals (EDCs). During pregnancy, the expression of steroid hormone receptors is controlled by maternal and fetal nutrition. To evaluate the impact of EDCs during pregnancy, ethinyl estradiol (EE, 0.2 mg/kg/day), OP (50 mg/kg/day), and BPA (50 mg/kg/day) were administered to pregnant mice. The mRNA levels of TRPV6 (transient receptor potential cation channels in subfamily V, member 6) decreased significantly by EE and OP. The PMCA1 (ATPase, Ca(++) transporting, plasma membrane 1) mRNA and protein levels decreased significantly by EE, OP, and BPA...
2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27623342/changes-in-intracellular-copper-concentration-and-copper-regulating-gene-expression-after-pc12-differentiation-into-neurons
#5
Yasumitsu Ogra, Aya Tejima, Naohiro Hatakeyama, Moeko Shiraiwa, Siyuan Wu, Tsutomu Ishikawa, Ayako Yawata, Yasumi Anan, Noriyuki Suzuki
It is suspected that some neurodegenerative diseases are a result of the disturbance of copper (Cu) homeostasis, although it remains unclear whether the disturbance of Cu homeostasis has aberrant effects on neurons. Herein, we investigated Cu metabolism specifically in neurons in terms of changes in the intracellular Cu concentration and the expression of Cu-regulating genes, such as Cu transporters and metallothioneins (MTs), before and after the differentiation of rat pheochromocytoma cells (PC12 cells) into neurons...
September 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27516958/transcriptome-analysis-of-copper-homeostasis-genes-reveals-coordinated-upregulation-of-slc31a1-sco1-and-cox11-in-colorectal-cancer
#6
Vincenza Barresi, Angela Trovato-Salinaro, Giorgia Spampinato, Nicolò Musso, Sergio Castorina, Enrico Rizzarelli, Daniele Filippo Condorelli
Copper homeostasis and distribution is strictly regulated by a network of transporters and intracellular chaperones encoded by a group of genes collectively known as copper homeostasis genes (CHGs). In this work, analysis of The Cancer Genome Atlas database for somatic point mutations in colorectal cancer revealed that inactivating mutations are absent or extremely rare in CHGs. Using oligonucleotide microarrays, we found a strong increase in mRNA levels of the membrane copper transporter 1 protein [CTR1; encoded by the solute carrier family 31 member 1 gene (SLC31A1 gene)] in our series of colorectal carcinoma samples...
August 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27509383/effects-of-waterborne-cu-exposure-on-intestinal-copper-transport-and-lipid-metabolism-of-synechogobius-hasta
#7
Feng Chen, Zhi Luo, Guang-Hui Chen, Xi Shi, Xu Liu, Yu-Feng Song, Ya-Xiong Pan
The present study was conducted to explore the effects of waterborne Cu exposure on intestinal Cu transport and lipid metabolism of Synechogobius hasta. S. hasta were exposed to 0, 0.4721 and 0.9442μM Cu, respectively. Sampling occurred on days 0, 21 and 42, respectively. Growth performance, intestinal lipid deposition, Cu content, and activities and mRNA expression of enzymes and genes involved in Cu transport and lipid metabolism were analyzed. Cu exposure decreased WG and SGR on days 21 and 42. Cu exposure increased intestinal Cu and lipid contents...
September 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27426920/yeast-membrane-proteomics-using-leucine-metabolic-labelling-bioinformatic-data-processing-and-exemplary-application-to-the-er-intramembrane-protease-ypf1
#8
Lars Nilse, Dönem Avci, Patrick Heisterkamp, Oliver Serang, Marius K Lemberg, Oliver Schilling
We describe in detail the usage of leucine metabolic labelling in yeast in order to monitor quantitative proteome alterations, e.g. upon removal of a protease. Since laboratory yeast strains are typically leucine auxotroph, metabolic labelling with trideuterated leucine (d3-leucine) is a straightforward, cost-effective, and ubiquitously applicable strategy for quantitative proteomic studies, similar to the widely used arginine/lysine metabolic labelling method for mammalian cells. We showcase the usage of advanced peptide quantification using the FeatureFinderMultiplex algorithm (part of the OpenMS software package) for robust and reliable quantification...
October 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27374085/dextran-catechin-an-anticancer-chemically-modified-natural-compound-targeting-copper-that-attenuates-neuroblastoma-growth
#9
Orazio Vittorio, Miriam Brandl, Giuseppe Cirillo, Kathleen Kimpton, Elizabeth Hinde, Katharina Gaus, Eugene Yee, Naresh Kumar, Hien Duong, Claudia Fleming, Michelle Haber, Murray Norris, Cyrille Boyer, Maria Kavallaris
Neuroblastoma is frequently diagnosed at advanced stage disease and treatment includes high dose chemotherapy and surgery. Despite the use of aggressive therapy survival rates are poor and children that survive their disease experience long term side effects from their treatment, highlighting the need for effective and less toxic therapies. Catechin is a natural polyphenol with anti-cancer properties and limited side effects, however its mechanism of action is unknown. Here we report that Dextran-Catechin, a conjugated form of catechin that increases serum stability, is preferentially and markedly active against neuroblastoma cells having high levels of intracellular copper, without affecting non-malignant cells...
June 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27362522/host-imposed-copper-poisoning-impacts-fungal-micronutrient-acquisition-during-systemic-candida-albicans-infections
#10
Joanna Mackie, Edina K Szabo, Dagmar S Urgast, Elizabeth R Ballou, Delma S Childers, Donna M MacCallum, Joerg Feldmann, Alistair J P Brown
Nutritional immunity is a process whereby an infected host manipulates essential micronutrients to defend against an invading pathogen. We reveal a dynamic aspect of nutritional immunity during infection that involves copper assimilation. Using a combination of laser ablation inductively coupled mass spectrometry (LA-ICP MS) and metal mapping, immunohistochemistry, and gene expression profiling from infected tissues, we show that readjustments in hepatic, splenic and renal copper homeostasis accompany disseminated Candida albicans infections in the mouse model...
2016: PloS One
https://www.readbyqxmd.com/read/27331785/loss-of-divalent-metal-transporter-1-function-promotes-brain-copper-accumulation-and-increases-impulsivity
#11
Murui Han, JuOae Chang, Jonghan Kim
The divalent metal transporter 1 (DMT1) is a major iron transporter required for iron absorption and erythropoiesis. Loss of DMT1 function results in microcytic anemia. While iron plays an important role in neural function, the behavioral consequences of DMT1 deficiency are largely unexplored. The goal of this study was to define the neurobehavioral and neurochemical phenotypes of homozygous Belgrade (b/b) rats that carry DMT1 mutation and explore potential mechanisms of these phenotypes. The b/b rats (11-12 weeks old) and their healthy littermate heterozygous (+/b) Belgrade rats were subject to elevated plus maze tasks...
September 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27323292/five-metal-elements-homeostasis-related-genes-in-synechogobius-hasta-molecular-characterization-tissue-expression-and-transcriptional-response-to-cu-and-fe-exposure
#12
Feng Chen, Zhi Luo, Yao-Fang Fan, Kun Wu, Ya-Xiong Pan, Xu Liu, Li-Han Zhang, Yu-Feng Song
Two isoforms of Cu transporter (CTR1 and CTR2) and metallothionein (MT1 and MT2), and divalent metal ion transporter 1 (DMT1) were cloned and characterized in Synechogobius hasta, respectively. The protein sequences of S. hasta CTRs possessed two methionine-rich regions (MxM and MxxxM) and three transmembrane regions. At the C-terminus, CTR1 contained a sequence of conserved cysteine and histidine residues (HCH), while CTR2 did not contain the conserved sequence. The protein sequence of S. hasta DMT1 possessed all the characteristic features of DMT1, including twelve conserved hydrophobic cores of transmembrane domains...
September 2016: Chemosphere
https://www.readbyqxmd.com/read/27270317/neat1-upregulates-egcg-induced-ctr1-to-enhance-cisplatin-sensitivity-in-lung-cancer-cells
#13
Pan Jiang, Xiaoyue Wu, Xuemin Wang, Wenbin Huang, Qing Feng
Platinum-based drugs are the firstline of treatment for non-small cell lung cancer (NSCLC), but resistance to these drugs is a major obstacle to effective chemotherapy. Our previous study revealed that the green tea polyphenol, EGCG, induced cisplatin transporter CTR1 (copper transporter 1) and enhanced cisplatin sensitivity in ovarian cancer. In this study, we found that EGCG upregulated CTR1 and increased platinum accumulation in NSCLC (A549, H460 and H1299) cells, cDDP-resistant A549 cells and a nude mouse xenograft model...
May 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27268962/maize-and-arabidopsis-argos-proteins-interact-with-ethylene-receptor-signaling-complex-supporting-a-regulatory-role-for-argos-in-ethylene-signal-transduction
#14
Jinrui Shi, Bruce J Drummond, Hongyu Wang, Rayeann L Archibald, Jeffrey E Habben
The phytohormone ethylene regulates plant growth and development as well as plant response to environmental cues. ARGOS genes reduce plant sensitivity to ethylene when overexpressed in transgenic Arabidopsis (Arabidopsis thaliana) and maize (Zea mays). A previous genetic study suggested that the endoplasmic reticulum and Golgi-localized maize ARGOS1 targets the ethylene signal transduction components at or upstream of CONSTITUTIVE TRIPLE RESPONSE1, but the mechanism of ARGOS modulating ethylene signaling is unknown...
August 2016: Plant Physiology
https://www.readbyqxmd.com/read/27242886/ethylene-antagonizes-salt-induced-growth-retardation-and-cell-death-process-via-transcriptional-controlling-of-ethylene-bag-and-senescence-associated-genes-in-arabidopsis
#15
Ya-Jie Pan, Ling Liu, Ying-Chao Lin, Yuan-Gang Zu, Lei-Peng Li, Zhong-Hua Tang
The existing question whether ethylene is involved in the modulation of salt-induced cell death to mediate plant salt tolerance is important for understanding the salt tolerance mechanisms. Here, we employed Arabidopsis plants to study the possible role of ethylene in salt-induced growth inhibition and programmed cell death (PCD) profiles. The root length, DNA ladder and cell death indicated by Evan's blue detection were measured by compared to the control or salt-stressed seedlings. Secondly, the protoplasts isolated from plant leaves and dyed with Annexin V-FITC were subjected to flow cytometric (FCM) assay...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27215645/a-natural-frameshift-mutation-in-campanula-eil2-correlates-with-ethylene-insensitivity-in-flowers
#16
Line Jensen, Josefine Nymark Hegelund, Andreas Olsen, Henrik Lütken, Renate Müller
BACKGROUND: The phytohormone ethylene plays a central role in development and senescence of climacteric flowers. In ornamental plant production, ethylene sensitive plants are usually protected against negative effects of ethylene by application of chemical inhibitors. In Campanula, flowers are sensitive to even minute concentrations of ethylene. RESULTS: Monitoring flower longevity in three Campanula species revealed C. portenschlagiana (Cp) as ethylene sensitive, C...
May 23, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27157188/copper-transporters-and-chaperones-ctr1-ctr2-atox1-and-ccs-as-determinants-of-cisplatin-sensitivity
#17
Kristin M Bompiani, Cheng-Yu Tsai, Felix P Achatz, Janika K Liebig, Stephen B Howell
The development of resistance to cisplatin (cDDP) is commonly accompanied by reduced drug uptake or increased efflux. Previous studies in yeast and murine embryonic fibroblasts have reported that the copper (Cu) transporters and chaperones participate in the uptake, efflux, and intracellular distribution of cDDP. However, there is conflicting data from studies in human cells. We used CRISPR-Cas9 genome editing to individually knock out the human copper transporters CTR1 and CTR2 and the copper chaperones ATOX1 and CCS...
September 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27143361/cathepsin-protease-controls-copper-and-cisplatin-accumulation-via-cleavage-of-the-ctr1-metal-binding-ectodomain
#18
Helena Öhrvik, Brandon Logeman, Boris Turk, Thomas Reinheckel, Dennis J Thiele
Copper is an essential metal ion for embryonic development, iron acquisition, cardiac function, neuropeptide biogenesis, and other critical physiological processes. Ctr1 is a high affinity Cu(+) transporter on the plasma membrane and endosomes that exists as a full-length protein and a truncated form of Ctr1 lacking the methionine- and histidine-rich metal-binding ectodomain, and it exhibits reduced Cu(+) transport activity. Here, we identify the cathepsin L/B endolysosomal proteases functioning in a direct and rate-limiting step in the Ctr1 ectodomain cleavage...
July 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27118085/potential-of-curcumin-mediated-photodynamic-inactivation-to-reduce-oral-colonization
#19
Carolina Santezi, Juliane M G Tanomaru, Vanderlei S Bagnato, Osmir B Oliveira Júnior, Lívia N Dovigo
OBJECTIVE: The present study assessed the susceptibility of salivary pathogens to photodynamic inactivation (PDI), mediated by a water-soluble mixture of curcuminoids (CRM) and LED light. METHODS: A 10mL sample of unstimulated saliva was collected from volunteers. The inoculum was prepared using 9mL of saline and 1mL of saliva. Inoculum suspensions were divided into 14 groups and treated according to the description below. Groups that received the PDI treatment (light for 1min or 5min and 1...
September 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27112899/dual-role-of-lrrc8a-containing-transporters-on-cisplatin-resistance-in-human-ovarian-cancer-cells
#20
Belinda Halling Sørensen, Celina Støving Dam, Stefan Stürup, Ian Henry Lambert
Acquired resistance to chemotherapeutic drugs in cancer cells can reflect an ability to limit cellular drug availability, to repair drug induced DNA damage, and to limit initiation/progression of cell death (apoptosis). The leucine-rich-repeat-containing 8A (LRRC8A) protein is an essential component of volume sensitive channels for organic osmolytes (VSOAC) and volume regulated anion channels (VRAC), which are activated during the apoptotic process. Here we illustrate that cisplatin resistance in human ovarian cancer cells (A2780) correlates with a reduced expression of LRRC8A and copper transporter receptor 1 (CTR1), as well as a concomitant increased expression of copper-transporting P-type ATPases (ATP7A/ATP7B)...
July 2016: Journal of Inorganic Biochemistry
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