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https://www.readbyqxmd.com/read/29784876/chca-1-is-a-copper-regulated-ctr1-homolog-required-for-normal-development-copper-accumulation-and-copper-sensing-behavior-in-caenorhabditis-elegans
#1
Sai Yuan, Anuj Kumar Sharma, Alexandria Richart, Jaekwon Lee, Byung-Eun Kim
Copper (Cu) plays key roles in catalytic and regulatory biochemical reactions essential for normal growth, development, and health. Dietary Cu deficiencies or mutations in Cu homeostasis genes can lead to abnormal musculoskeletal development, cognitive disorders, and poor growth. In yeast and mammals, Cu is acquired through the activities of the Ctr1 family of high-affinity Cu transporters. However, the mechanisms of systemic responses to dietary or tissue-specific Cu deficiency remain unclear. Here, taking advantage of the animal model Caenorhabditis elegans for studying whole-body Cu homeostasis, we investigated the role of a C...
May 21, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29783005/metal-accumulation-and-expression-of-genes-encoding-for-metallothionein-and-copper-transporters-in-a-chronically-exposed-wild-population-of-the-fish-hyphessobrycon-luetkenii
#2
Sandra Isabel Moreno Abril, Patrícia Gomes Costa, Adalto Bianchini
In the present study, metal (As, Cd, Cu, Fe, Mn, Pb and Zn) accumulation and expression of genes involved in metal metabolism (metallothioneins, ATP7A and CTR1) were evaluated in gills and muscle of the fish Hyphessobrycon luetkenii living in the João Dias creek, a site historically (~1870-1996) contaminated with a metal mixture associated with copper mining (Minas do Camaquã, southern Brazil). Fish were collected in a metal impacted site of the João Dias creek and kept in a cage at this site (PP fish) or translocated to a non-metal impacted reach of this creek (PC fish)...
May 18, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29754608/multiplex-polymerase-chain-reaction-in-combination-with-gel-electrophoresis-inductively-coupled-plasma-mass-spectrometry-a-powerful-tool-for-the-determination-of-gene-copy-number-variations-and-gene-expression-changes
#3
A Fernández Asensio, T Iglesias, A Cotarelo, M Espina, E Blanco-González, L M Sierra, M Montes-Bayón
During the last few years multiplex real-time or quantitative polymerase chain reaction PCR (qPCR) has become the method of choice for multiplex gene expression changes and gene copy number variations (CNVs) analysis. However, such determinations require the use of different fluorescent labels for the different amplified sequences, which increases significantly the costs of the analysis and limits the applicability of the technique for simultaneous amplification of many targets of interest in a single reaction...
September 6, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29737086/-basic-research-of-the-adenovirus-mediated-hctr1-transfection-on-the-treatment-of-cisplatin-resistant-cervical-cancer
#4
Xiu-Li Wu, Tao Guo, Hua-Wei Cai
OBJECTIVE: To induce cisplatin-resistant cervical squamous carcinoma cell line and investigate the drug resistant mechanisms and adenovirus trans-gene therapeutical treatment. METHODS: The cisplatin-resistant subline,designated C-33A/cis,was originated by growing parental C-33A cells with gradually increasing doses of cisplatin. The cytotoxicity of cisplatin was determined by CCK-8 assay,and the CTR1 expression was measured by Western blot. Subcutaneous xenograft cervical tumor model was established by cisplatin-resistant C-33A/cis cell line...
January 2018: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29723715/expression-of-genes-related-to-metal-metabolism-in-the-freshwater-fish-hyphessobrycon-luetkenii-living-in-a-historically-contaminated-area-associated-with-copper-mining
#5
Sandra Isabel Moreno Abril, Camila Dalmolin, Patrícia Gomes Costa, Adalto Bianchini
Copper (Cu) mining in Minas do Camaquã-Brazil, released significant amounts of metals into the João Dias creek, where Hyphessobrycon luetkenii inhabit. Because the involvement of Cu in biological processes its concentration and availability is regulated by molecules as the metal regulatory transcription factor (MTF-1), metallothionein (MT) and transporters (ATP7A and CTR1). These genes were whole sequenced and their expression (GE) evaluated in gills, liver and intestine. Were collected fish in non-contaminated and contaminated (Cu 3...
April 26, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29643073/the-plant-hormone-ethylene-restricts-arabidopsis-growth-via-the-epidermis
#6
Irina Ivanova Vaseva, Enas Qudeimat, Thomas Potuschak, Yunlong Du, Pascal Genschik, Filip Vandenbussche, Dominique Van Der Straeten
The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2 ) target the degradation of EIN3, the master transcription factor in ethylene signaling...
April 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29618620/orthoxenografts-of-testicular-germ-cell-tumors-demonstrate-genomic-changes-associated-with-cisplatin-resistance-and-identify-pdmp-as-a-re-sensitizing-agent
#7
Josep Maria Piulats, August Vidal, Francisco J García-Rodríguez, Clara Muñoz, Marga Nadal, Catia Moutinho, Maria Martínez-Iniesta, Josefina Mora, Agnes Figueras, Elisabet Guinó, Laura Padullés, Àlvaro Aytés, David G Mollevi, Sara Puertas, Carmen Martínez-Fernández, Wilmar Castillo, Merce Juliachs, Victor Moreno, Purificacion Muñoz, Milica Stefanovic, Miguel Angel Pujana, Enric Condom, Manel Esteller, Josep R Germà-LLuch, Gabriel Capella, Lourdes Farré, Albert Morales, Francesc Viñals, Xavier Garcia Del Muro, Julián Cerón, Alberto Villanueva
PURPOSE: To investigate the genetic basis of cisplatin resistance. Efficacy of cisplatin chemotherapy in the treatment of distinct malignancies is often hampered by intrinsic/ acquired drug resistance of tumors. EXPERIMENTAL DESIGN: We produced 14 orthoxenograft transplanting human nonseminomatous (NSE) testicular germ cell tumors (TGCTs) to mice, keeping the primary tumor features (genotype, phenotype and sensitivity to cisplatin). Chromosomal and genetic alterations were evaluated in matched cisplatin-sensitive and their counterpart orthoxenografts that developed resistance to cisplatin in vivo...
April 4, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29608794/genome-wide-analysis-of-the-regulation-of-cu-metabolism-in-cryptococcus-neoformans
#8
Sarela Garcia-Santamarina, Richard A Festa, Aaron D Smith, Chen-Hsin Yu, Corinna Probst, Chen Ding, Christina M Homer, Jun Yin, James P Noonan, Hiten Madhani, John R Perfect, Dennis J Thiele
The ability of the human fungal pathogen Cryptococcus neoformans to adapt to variable copper (Cu) environments within the host is key for successful dissemination and colonization. During pulmonary infection, host alveolar macrophages compartmentalize Cu into the phagosome and C. neoformans Cu-detoxifying metallothioneins, MT1 and MT2, are required for survival of the pathogen. In contrast, during brain colonization the C. neoformans Cu+ importers Ctr1 and Ctr4 are required for virulence. Central for the regulation and expression of both the Cu detoxifying MT1/2 and the Cu acquisition Ctr1/4 proteins is the Cu-metalloregulatory transcription factor Cuf1, an established C...
April 2, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29579719/copper-accumulation-in-senescent-cells-interplay-between-copper-transporters-and-impaired-autophagy
#9
Shashank Masaldan, Sharnel A S Clatworthy, Cristina Gamell, Zoe M Smith, Paul S Francis, Delphine Denoyer, Peter M Meggyesy, Sharon La Fontaine, Michael A Cater
Cellular senescence is characterized by irreversible growth arrest incurred through either replicative exhaustion or by pro-oncogenic cellular stressors (radioactivity, oxidative stress, oncogenic activation). The enrichment of senescent cells in tissues with age has been associated with tissue dyshomeostasis and age-related pathologies including cancers, neurodegenerative disorders (e.g. Alzheimer's, Parkinson's, etc.) and metabolic disorders (e.g. diabetes). We identified copper accumulation as being a universal feature of senescent cells [mouse embryonic fibroblasts (MEF), human prostate epithelial cells and human diploid fibroblasts] in vitro...
March 17, 2018: Redox Biology
https://www.readbyqxmd.com/read/29515596/genomic-sequencing-of-japanese-plum-prunus-salicina-lindl-mutants-provides-a-new-model-for-rosaceae-fruit-ripening-studies
#10
Angel Fernandez I Marti, Christopher A Saski, George A Manganaris, Ksenija Gasic, Carlos H Crisosto
It has recently been described that the Japanese plum "Santa Rosa" bud sport series contains variations in ripening pattern: climacteric, suppressed-climacteric and non-climacteric types. This provides an interesting model to study the role of ethylene and other key mechanisms governing fruit ripening, softening and senescence. The aim of the current study was to investigate such differences at the genomic level, using this series of plum bud sports, with special reference to genes involved in ethylene biosynthesis, signal transduction, and sugar metabolism...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29507693/copper-myc-ctr1-interplay-a-dangerous-relationship-in-hepatocellular-carcinoma
#11
Cristiana Porcu, Laura Antonucci, Barbara Barbaro, Barbara Illi, Sergio Nasi, Maurizio Martini, Anna Licata, Luca Miele, Antonio Grieco, Clara Balsano
Free serum copper correlates with tumor incidence and progression of human cancers, including hepatocellular carcinoma (HCC). Copper extracellular uptake is provided by the transporter CTR1, whose expression is regulated to avoid excessive intracellular copper entry. Inadequate copper serum concentration is involved in the pathogenesis of Non Alcoholic Fatty Liver Disease (NAFLD), which is becoming a major cause of liver damage progression and HCC incidence. Finally, MYC is over-expressed in most of HCCs and is a critical regulator of cellular growth, tumor invasion and metastasis...
February 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29495260/pilot-study-of-64-cucl%C3%A2-for-pet-imaging-of-inflammation
#12
Lei Jiang, Dongli Song, Hao Chen, Ao Zhang, Huoqiang Wang, Zhen Cheng
Copper(II) ion (Cu2+ ) is the essential element for numerous pathophysiological processes in vivo. Copper transporter 1 (CTR1) is mainly responsible for maintaining Cu2+ accumulation in cells, which has been found to be over-expressed in inflammatory tissues. Therefore, we explored the potential application of 64 CuCl₂ for PET imaging of inflammation through targeting CTR1. The animal models of H₂O₂ induced muscle inflammation and lipopolysaccaharide induced lung inflammation were successfully established, then imaged by small animal PET (PET/CT) post-injection of 64 CuCl₂, and PET images were quantitatively analyzed...
February 24, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29479997/correlation-of-expression-levels-of-copper-transporter-1-and-thymidylate-synthase-with-treatment-outcomes-in-patients-with-advanced-non-small-cell-lung-cancer-treated-with-s-1-carboplatin-doublet-chemotherapy
#13
Maho Konishi, Asuka Imai, Masanori Fujii, Keisuke Sugimoto, Nobuyuki Katakami, Yukihiro Imai, Shingo Kamoshida
Background: Copper transporter 1 (CTR1) is a critical determinant of the uptake and cytotoxic effect of the platinum drugs carboplatin and cisplatin. Thymidylate synthase (TS) is an enzyme involved in DNA synthesis and is associated with resistance of tumor cells to 5-fluorouracil. We investigated the correlation between CTR1 and TS expression levels and treatment outcomes in patients with advanced non-small-cell lung cancer (NSCLC) treated with S-1/carboplatin doublet chemotherapy. Methods: Twenty-nine patients were enrolled in this study...
February 26, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29477856/copper-homeostasis-as-a-target-to-improve-saccharomyces-cerevisiae-tolerance-to-oxidative-stress
#14
Nadia Maria Berterame, Francesca Martani, Danilo Porro, Paola Branduardi
The yeast Saccharomyces cerevisiae is widely used as a cell factory for the biotechnological production of various industrial products. During these processes, yeasts meet different kinds of stressors that often cause oxidative stress and thus impair cell growth. Therefore, the development of robust strains is indispensable to improve production, yield and productivity of fermentative processes. Copper plays a key role in the response to oxidative stress, as cofactor of the cytosolic superoxide dismutase (Sod1) and being contained in metallochaperone and metallothioneines with antioxidant properties...
March 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29417972/relevance-of-copper-transporter-1-and-organic-cation-transporters-1-3-for-oxaliplatin-uptake-and-drug-resistance-in-colorectal-cancer-cells
#15
I Buß, A Hamacher, N Sarin, M U Kassack, G V Kalayda
Oxaliplatin is a routinely used drug in the treatment of colorectal cancer. However, development of resistance is a major hurdle of the chemotherapy success. Defects in cellular accumulation represent a frequently reported feature of cells with acquired resistance to platinum drugs. Nevertheless, the mechanisms of oxaliplatin uptake and their role in oxaliplatin resistance remain poorly elucidated. The aim of this study was to investigate the relevance of copper transporter 1 (CTR1) and organic cation transporters 1-3 (OCT1-3) for oxaliplatin uptake and resistance to the drug in sensitive and oxaliplatin-resistant ileocecal colorectal adenocarcinoma cells...
March 1, 2018: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29402466/the-cu-ii-affinity-of-the-n-terminus-of-human-copper-transporter-ctr1-comparison-of-human-and-mouse-sequences
#16
Karolina Bossak, Simon C Drew, Ewelina Stefaniak, Dawid Płonka, Arkadiusz Bonna, Wojciech Bal
Copper Transporter 1 (CTR1) is a homotrimeric membrane protein providing the main route of copper transport into eukaryotic cells from the extracellular milieu. Its N-terminal extracellular domain, rich in His and Met residues, is considered responsible for directing copper into the transmembrane channel. Most of vertebrate CTR1 proteins contain the His residue in position three from N-terminus, creating a well-known Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) site. CTR1 from humans, primates and many other species contains the Met-Asp-His (MDH) sequence, while some rodents including mouse have the Met-Asn-His (MNH) N-terminal sequence...
May 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29301278/theaflavin-3-3-digallate-enhances-the-inhibitory-effect-of-cisplatin-by-regulating-the-copper-transporter-1-and-glutathione-in-human-ovarian-cancer-cells
#17
Haibo Pan, Eunhye Kim, Gary O Rankin, Yon Rojanasakul, Youying Tu, Yi Charlie Chen
Ovarian cancer has the highest fatality rate among the gynecologic cancers. The side effects, high relapse rate, and drug resistance lead to low long-term survival rate (less than 40%) of patients with advanced ovarian cancer. Theaflavin-3,3'-digallate (TF3), a black tea polyphenol, showed less cytotoxicity to normal ovarian cells than ovarian cancer cells. We aimed to investigate whether TF3 could potentiate the inhibitory effect of cisplatin against human ovarian cancer cell lines. In the present study, combined treatment with TF3 and cisplatin showed a synergistic cytotoxicity against A2780/CP70 and OVCAR3 cells...
January 2, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29192345/transporter-mediated-interaction-between-platinum-drugs-and-sorafenib-at-the-cellular-level
#18
Verena Schneider, Selim Chaib, Claudia Spanier, Mandy Knapp, Violeta Moscvin, Laura Scordovillo, Alessandra Ewertz, Ulrich Jaehde, Ganna V Kalayda
Combining the multikinase inhibitor sorafenib with the platinum-based chemotherapy of solid tumors was expected to improve treatment outcome. However, in many clinical trials, no benefit from sorafenib addition to the platinum-containing regimen could be demonstrated. Moreover, in some studies, decreased survival of ovarian cancer patients as well as non-small cell lung cancer patients with squamous cell histology was observed. The aim of this study was to investigate the cellular mechanisms of the pharmacological interaction between platinum drugs and sorafenib in different cancer cell lines...
November 30, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29190774/activation-of-the-cholinergic-anti-inflammatory-pathway-by-gts-21-attenuates-cisplatin-induced-acute-kidney-injury-in-mice
#19
Prodyot K Chatterjee, Michael M Yeboah, Malvika H Solanki, Gopal Kumar, Xiangying Xue, Valentin A Pavlov, Yousef Al-Abed, Christine N Metz
Acute kidney injury (AKI) is the most common side effect of cisplatin, a widely used chemotherapy drug. Although AKI occurs in up to one third of cancer patients receiving cisplatin, effective renal protective strategies are lacking. Cisplatin targets renal proximal tubular epithelial cells leading to inflammation, reactive oxygen species, tubular cell injury, and eventually cell death. The cholinergic anti-inflammatory pathway is a vagus nerve-mediated reflex that suppresses inflammation via α7 nicotinic acetylcholine receptors (α7nAChRs)...
2017: PloS One
https://www.readbyqxmd.com/read/29152741/neat1-acts-as-an-inducer-of-cancer-stem-cell-like-phenotypes-in-nsclc-by-inhibiting-egcg-upregulated-ctr1
#20
Pan Jiang, Aochang Chen, Xiaoyue Wu, Ming Zhou, Ijaz Ul Haq, Zahula Mariyam, Qing Feng
Long non-coding RNAs (lncRNAs) play significant roles in the pathogenesis of various cancers, including lung cancer. In this study, we aimed to investigate the biological function of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in cancer stem cells (CSCs). CSCs have been suggested as the main cause of tumor metastasis, tumor recurrence, and chemotherapy resistance. The copper transporter 1 (CTR1) has been the focus of many recent studies because of its correlation with cisplatin (CDDP) resistance. So far, the mechanism of how NEAT1 regulates CSCs in NSCLC remains unknown...
June 2018: Journal of Cellular Physiology
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