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Gillian Murphy
To many of us in the field, working on Matrix Metalloproteinases (MMPs) has felt like riding a roller coaster, traveling through times of both excitement and despair. I was fortunate to join the ride when it was a mere carousel of three activities thought to target the proteins that comprise the extracellular matrix (ECM). New technologies brought the thrills of discovery, as we uncovered specific proteinase genes and defined specialised activities in different cellular processes. The MMPs and the sister families of 'A Disintegrin And Metalloproteinase' (ADAMs), ADAMs with ThromboSpondin domains (ADAM-TS) and Astacins are now recognised as key signalling 'scissors' that drive rapid changes in a plethora of cellular pathways...
March 15, 2017: Journal of Biological Chemistry
Ádám Butykai, Sándor Bordács, István Kézsmárki, Vladimir Tsurkan, Alois Loidl, Jonathan Döring, Erik Neuber, Peter Milde, Susanne C Kehr, Lukas M Eng
GaV4S8 is a multiferroic semiconductor hosting Néel-type magnetic skyrmions dressed with electric polarization. At Ts = 42 K, the compound undergoes a structural phase transition of weakly first-order, from a non-centrosymmetric cubic phase at high temperatures to a polar rhombohedral structure at low temperatures. Below Ts, ferroelectric domains are formed with the electric polarization pointing along any of the four 〈111〉 axes. Although in this material the size and the shape of the ferroelectric-ferroelastic domains may act as important limiting factors in the formation of the Néel-type skyrmion lattice emerging below TC = 13 K, the characteristics of polar domains in GaV4S8 have not been studied yet...
March 15, 2017: Scientific Reports
Damayanti Chakraborty, Wei Cui, Gracy X Rosario, Regan L Scott, Pramod Dhakal, Stephen J Renaud, Makoto Tachibana, M A Karim Rumi, Clifford W Mason, Adam J Krieg, Michael J Soares
The hemochorial placenta develops from the coordinated multilineage differentiation of trophoblast stem (TS) cells. An invasive trophoblast cell lineage remodels uterine spiral arteries, facilitating nutrient flow, failure of which is associated with pathological conditions such as preeclampsia, intrauterine growth restriction, and preterm birth. Hypoxia plays an instructive role in influencing trophoblast cell differentiation and regulating placental organization. Key downstream hypoxia-activated events were delineated using rat TS cells and tested in vivo, using trophoblast-specific lentiviral gene delivery and genome editing...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
Magdalena A Mozolewska, Paweł Krupa, Bartłomiej Zaborowski, Adam Liwo, Jooyoung Lee, Keehyoung Joo, Cezary Czaplewski
Recently, we developed a new approach to protein-structure prediction, which combines template-based modeling with the physics-based coarse-grained UNited RESidue (UNRES) force field. In this approach, restrained multiplexed replica exchange molecular dynamics simulations with UNRES, with the C(α)-distance and virtual-bond-dihedral-angle restraints derived from knowledge-based models are carried out. In this work, we report a test of this approach in the 11th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP11), in which we used the template-based models from early-stage predictions by the LEE group CASP11 server (group 038, called "nns"), and further improvement of the method...
November 28, 2016: Journal of Chemical Information and Modeling
Adam Stevens, Philip Murray, Jerome Wojcik, John Raelson, Ekaterina Koledova, Pierre Chatelain, Peter Clayton
OBJECTIVE: Single-nucleotide polymorphisms (SNPs) associated with the response to recombinant human growth hormone (r-hGH) have previously been identified in growth hormone deficiency (GHD) and Turner syndrome (TS) children in the PREDICT long-term follow-up (LTFU) study (Nbib699855). Here, we describe the PREDICT validation (VAL) study (Nbib1419249), which aimed to confirm these genetic associations. DESIGN AND METHODS: Children with GHD (n = 293) or TS (n = 132) were recruited retrospectively from 29 sites in nine countries...
December 2016: European Journal of Endocrinology
Tuomas K Niemi, Melissa A Marchesan, Adam Lloyd, Robert J Seltzer
INTRODUCTION: The aim of this study was to compare the effectiveness of TRUShape (TS) instruments with ProFile Vortex Blue (VB) instruments for the removal of obturation materials during retreatment of single-canal mandibular premolars performed through 2 access outlines. METHODS: Initial root canal treatment was completed through a contracted endodontic cavity (CEC) design. Canals were instrumented to an F2 ProTaper instrument, obturated with warm lateral condensation of gutta-percha with AH Plus sealer, and allowed to set for 30 days at 37°C and 100% humidity...
October 2016: Journal of Endodontics
Clorice R Reinhardt, Tanner C Jaglinski, Ashly M Kastenschmidt, Eun H Song, Adam K Gross, Alyssa J Krause, Jonathan M Gollmar, Kristin J Meise, Zachary S Stenerson, Tyler J Weibel, Andrew Dison, Mackenzie R Finnegan, Daniel S Griesi, Michael D Heltne, Tom G Hughes, Connor D Hunt, Kayla A Jansen, Adam H Xiong, Sanchita Hati, Sudeep Bhattacharyya
The kinetics and equilibrium of the hydride transfer reaction between lumiflavin and a number of substituted quinones was studied using density functional theory. The impact of electron withdrawing/donating substituents on the redox potentials of quinones was studied. In addition, the role of these substituents on the kinetics of the hydride transfer reaction with lumiflavin was investigated in detail under the transition state (TS) theory assumption. The hydride transfer reactions were found to be more favorable for an electron-withdrawing substituent...
September 2016: Journal of Molecular Modeling
Anna Antosiewicz, Adam Jarmuła, Dorota Przybylska, Grażyna Mosieniak, Joanna Szczepanowska, Anna Kowalkowska, Wojciech Rode, Joanna Cieśla
Enzymes involved in thymidylate biosynthesis, thymidylate synthase (TS), and dihydrofolate reductase (DHFR) are well-known targets in cancer chemotherapy. In this study, we demonstrated for the first time, that human TS and DHFR form a strong complex in vitro and co-localize in human normal and colon cancer cell cytoplasm and nucleus. Treatment of cancer cells with methotrexate or 5-fluorouracil did not affect the distribution of either enzyme within the cells. However, 5-FU, but not MTX, lowered the presence of DHFR-TS complex in the nucleus by 2...
August 5, 2016: Journal of Biomolecular Structure & Dynamics
Adam Czyzyk, Agnieszka Podfigurna-Stopa, Krzysztof Katulski, Grzegorz H Breborowicz, Andrea R Genazzani, Blazej Meczekalski
Patients suffering from Turner syndrome (TS) demonstrate characteristic clinical features, with a short stature and gonadal dysgenesis causing infertility in most patients. Spontaneous pregnancies in women with TS are quite rare and pregnancy outcomes involving an increased risk of miscarriage and stillbirths are observed. In this case report, we present a 28 years old pregnant woman with the diagnosis of TS. Due to hypergonadotrophic hypogonadism, she was proposed an in vitro fertilization (IVF) program with an oocyte donor from unrelated anonymous women...
August 2016: Gynecological Endocrinology
Ádám Madarász, Dénes Berta, Robert S Paton
Transition state force fields (TSFF) treated the TS structure as an artificial minimum on the potential energy surface in the past decades. The necessary parameters were developed either manually or by the Quantum-to-molecular mechanics method (Q2MM). In contrast with these approaches, here we propose to model the TS structures as genuine saddle points at the molecular mechanics level. Different methods were tested on small model systems of general chemical reactions such as protonation, nucleophilic attack, and substitution, and the new procedure led to more accurate models than the Q2MM-type parametrization...
April 12, 2016: Journal of Chemical Theory and Computation
Jan Ludwiczak, Piotr Maj, Piotr Wilk, Tomasz Frączyk, Tomasz Ruman, Borys Kierdaszuk, Adam Jarmuła, Wojciech Rode
Endogenous thymidylate synthases, isolated from tissues or cultured cells of the same specific origin, have been reported to show differing slow-binding inhibition patterns. These were reflected by biphasic or linear dependence of the inactivation rate on time and accompanied by differing inhibition parameters. Considering its importance for chemotherapeutic drug resistance, the possible effect of thymidylate synthase inhibition by post-translational modification was tested, e.g. phosphorylation, by comparing sensitivities to inhibition by two slow-binding inhibitors, 5-fluoro-dUMP and N(4)-hydroxy-dCMP, of two fractions of purified recombinant mouse enzyme preparations, phosphorylated and non-phosphorylated, separated by metal oxide/hydroxide affinity chromatography on Al(OH)3 beads...
April 2016: Molecular BioSystems
Roy Rabbie, David J Adams
No abstract text is available yet for this article.
March 2016: Pigment Cell & Melanoma Research
Tomasz Frączyk, Tomasz Ruman, Piotr Wilk, Paweł Palmowski, Adelina Rogowska-Wrzesinska, Joanna Cieśla, Zbigniew Zieliński, Joanna Nizioł, Adam Jarmuła, Piotr Maj, Barbara Gołos, Patrycja Wińska, Sylwia Ostafil, Elżbieta Wałajtys-Rode, David Shugar, Wojciech Rode
Thymidylate synthase (TS) may undergo phosphorylation endogenously in mammalian cells, and as a recombinant protein expressed in bacterial cells, as indicated by the reaction of purified enzyme protein with Pro-Q® Diamond Phosphoprotein Gel Stain (PGS). With recombinant human, mouse, rat, Trichinella spiralis and Caenorhabditis elegans TSs, expressed in Escherichia coli, the phosphorylated, compared to non-phosphorylated recombinant enzyme forms, showed a decrease in Vmax(app), bound their cognate mRNA (only rat enzyme studied), and repressed translation of their own and several heterologous mRNAs (human, rat and mouse enzymes studied)...
December 2015: Biochimica et Biophysica Acta
Marc D Coleman, Simon Render, Chris Dimopoulos, Adam Lilley, Rod A Robinson, Thomas O M Smith, Richard Camm, Rupert Standring
We compare the performance of an alternative method based on portable Fourier-transform infrared (FTIR) spectroscopy described in TGN M22, "Measuring Stack Gas Emissions Using FTIR Instruments," to the Standard Reference Methods (SRMs) for CO (EN 15058), NOx (EN 14792), SO₂(EN 14791), HCl (EN 1911), and H₂O (EN 14790). Testing was carried out using a Stack Simulator facility generating complex gas matrices of the measurands across concentration ranges of 0-75 mg m⁻³ and 0-100 mg m⁻³ CO, 0-200 mg m⁻³ and 0-300 mg m⁻³ NO, 0-75 mg m⁻³ and 0-200 mg m⁻³ SO₂, 0-15 mg m⁻³ and 0-60 mg m⁻³ HCl, and 0-14 vol% H₂O...
August 2015: Journal of the Air & Waste Management Association
Derek A Nichols, Jacqueline C Hargis, Ruslan Sanishvili, Priyadarshini Jaishankar, Kyle Defrees, Emmanuel W Smith, Kenneth K Wang, Fabio Prati, Adam R Renslo, H Lee Woodcock, Yu Chen
Ligand binding can change the pKa of protein residues and influence enzyme catalysis. Herein, we report three ultrahigh resolution X-ray crystal structures of CTX-M β-lactamase, directly visualizing protonation state changes along the enzymatic pathway: apo protein at 0.79 Å, precovalent complex with nonelectrophilic ligand at 0.89 Å, and acylation transition state (TS) analogue at 0.84 Å. Binding of the noncovalent ligand induces a proton transfer from the catalytic Ser70 to the negatively charged Glu166, and the formation of a low-barrier hydrogen bond (LBHB) between Ser70 and Lys73, with a length of 2...
July 1, 2015: Journal of the American Chemical Society
Alison Jeffery, Adam J Streeter, Joanne Hosking, Terence J Wilkin, Scott M Nelson
BACKGROUND: Anti-Müllerian hormone (AMH) is produced by Sertoli cells of the testes and granulosa cells of the ovary. There are limited prospective longitudinal data assessing AMH concentrations throughout childhood in both sexes. OBJECTIVE: This study aimed to examine AMH throughout childhood with particular reference to the relationship of AMH to pubertal development in both sexes. DESIGN: This is a prospective longitudinal non-intervention cohort study with annual sampling for participants aged 5-14 years...
September 2015: Journal of Pediatric Endocrinology & Metabolism: JPEM
Paul C Adams, James C Barton, Helen Guo, David Alter, Mark Speechley
BACKGROUND: We identified no reports of long-term follow-up of participants in hemochromatosis screening programs. We evaluated causes of death and survival in non-C282Y homozygous Canadian participants in the primary care-based hemochromatosis and iron overload screening (HEIRS) study. MATERIAL AND METHODS: Initial screening (IS) included transferrin saturation (TS), serum ferritin (SF), HFE genotyping (C282Y, H63D), and health questionnaire responses. By definition, participants without C282Y or H63D had HFE wt/wt...
May 2015: Annals of Hepatology
Marzena Maciejewska-Jeske, Adam Czyzyk, Blazej Meczekalski
BACKGROUND: Turner syndrome (TS) is a gonadal dysgenesis related to partial or total lack of one of the X chromosomes. It this report we describe a young patient presenting some somatic features of TS, who underwent spontaneous puberty and was eumenoorheic up to the age of 23. METHODS: Using fluorescent in situ hybridization (FISH) mosaic karyotype (45X[131]/47XXX[9]) of TS and triple X syndrome was found. RESULTS: She presented uncommon for TS somatic hemihypotrophy and underwent growth hormone and surgical therapy...
July 2015: Gynecological Endocrinology
Eric A Storch, Camille E Hanks, Jonathan W Mink, Joseph F McGuire, Heather R Adams, Erika F Augustine, Amy Vierhile, Alyssa Thatcher, Rebecca Bitsko, Adam B Lewin, Tanya K Murphy
OBJECTIVE: Despite evidence of elevated risk factors for suicidal thoughts and behavior in youth with Tourette syndrome and chronic tic disorders (CTD), few studies have actually examined that relationship. This study documented the frequency and clinical correlates of suicidal thoughts and behaviors in a sample of children and adolescents with CTD (N = 196, range 6-18 years old). The content is solely the responsibility of the authors and does not necessarily represent the official views of the Centers for Disease Control...
October 2015: Depression and Anxiety
Marcin Spychała, Aleksandra Sowińska, Justyna Starzyk, Adam Masłowski
The objective of this study was a preliminary identification of basic groups of micro-organisms in the cross-sectional profile of geotextile filters for septic tank effluent (STE) treatment and their relations to technological conditions. Reactors with textile filters treating wastewater were investigated on a semi-technical scale. Filters were vertically situated and STE was filtered through them under hydrostatic pressure at a wastewater surface height of 7-20 cm. Filters were made of four layers of non-woven TS 20 geotextile of 0...
July 2015: Environmental Technology
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