keyword
MENU ▼
Read by QxMD icon Read
search

elegans

keyword
https://www.readbyqxmd.com/read/29055282/do-developmental-temperatures-affect-redox-level-and-lifespan-in-c-elegans-through-upregulation-of-peroxiredoxin
#1
Dylan Henderson, Christian Huebner, Moses Markowitz, Nicole Taube, Zachary M Harvanek, Ursula Jakob, Daniela Knoefler
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25°C during development and shifted to 15°C in adulthood exhibited an even longer lifespan than animals constantly kept at 15°C. Analysis of the in vivo redox status demonstrated that at 25°C, C...
October 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/29053679/measuring-the-effect-of-chemicals-on-the-growth-and-reproduction-of-caenorhabditis-elegans
#2
So Young Lee, Kyungsu Kang
Toxicological evaluation is crucial for understanding the effects of chemicals on living organisms in basic and applied biological science fields. A non-mammalian soil round worm, Caenorhabditis elegans, is a valuable model organism for toxicology studies due to its convenience and lack of animal ethics issues compared with mammalian animal systems. In this protocol, a detailed procedure of toxicological evaluation of chemicals in C. elegans is described. A clinical anticancer drug, etoposide, which targets human topoisomerase II and inhibits DNA replication of human cancer cells, was selected as a model testing chemical...
October 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29051502/comparative-transcriptomics-reveal-developmental-turning-points-during-embryogenesis-of-a-hemimetabolous-insect-the-damselfly-ischnura-elegans
#3
Sabrina Simon, Sven Sagasser, Edoardo Saccenti, Mercer R Brugler, M Eric Schranz, Heike Hadrys, George Amato, Rob DeSalle
Identifying transcriptional changes during embryogenesis is of crucial importance for unravelling evolutionary, molecular and cellular mechanisms that underpin patterning and morphogenesis. However, comparative studies focusing on early/embryonic stages during insect development are limited to a few taxa. Drosophila melanogaster is the paradigm for insect development, whereas comparative transcriptomic studies of embryonic stages of hemimetabolous insects are completely lacking. We reconstructed the first comparative transcriptome covering the daily embryonic developmental progression of the blue-tailed damselfly Ischnura elegans (Odonata), an ancient hemimetabolous representative...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29050943/mir-67-regulates-p-aeruginosa-avoidance-behavior-in-c-elegans
#4
Yi-Cheng Ma, Lu Zhang, Li-Li Dai, Rahat Ullah Khan, Cheng-Gang Zou
Pathogen avoidance behaviors are found throughout the animal kingdom and are important for animal's survival in nature. As a free-living nematode, C. elegans is exposed to a variety of microorganisms, including toxic or pathogenic bacteria, in soil. C. elegans can develop efficient avoidance responses to pathogenic bacteria to minimize the infection risk. However, the role of microRNAs (miRNAs) in pathogen avoidance in C. elegans remains unclear. In this report, we showed that the miRNA mir-67 was involved in a behavioral avoidance response to P...
October 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29049853/nutrient-driven-o-glcnac-in-proteostasis-and-neurodegeneration
#5
REVIEW
Ilhan Akan, Stephanie Olivier-Van Stichelen, Michelle R Bond, John A Hanover
Proteostasis is essential in the mammalian brain where post-mitotic cells must function for decades to maintain synaptic contacts and memory. The brain is dependent on glucose and other metabolites for proper function and is spared from metabolic deficits even during starvation. In this review, we outline how the nutrient sensitive nucleocytoplasmic posttranslational modification O-linked N-acetylglucosamine (O-GlcNAc) regulates protein homeostasis. The O-GlcNAc modification is highly abundant in the mammalian brain, and has been linked to proteopathies, including neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's...
October 19, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29048752/the-how-and-where-of-plant-micrornas
#6
REVIEW
Yu Yu, Tianran Jia, Xuemei Chen
Contents Summary I. II. III. IV. References SUMMARY: MicroRNAs (miRNAs) are small non-coding RNAs, of typically 20-24 nt, that regulate gene expression post-transcriptionally through sequence complementarity. Since the identification of the first miRNA, lin-4, in the nematode Caenorhabditis elegans in 1993, thousands of miRNAs have been discovered in animals and plants, and their regulatory roles in numerous biological processes have been uncovered. In plants, research efforts have established the major molecular framework of miRNA biogenesis and modes of action, and are beginning to elucidate the mechanisms of miRNA degradation...
October 19, 2017: New Phytologist
https://www.readbyqxmd.com/read/29048526/comparative-transcriptomics-of-steinernema-and-caenorhabditis-single-embryos-reveals-orthologous-gene-expression-convergence-during-late-embryogenesis
#7
Marissa Macchietto, Dristi Angdembey, Negar Heidarpour, Lorrayne Serra, Bryan Rodriguez, Nicole El-Ali, Ali Mortazavi
Cells express distinct sets of genes in a precise spatio-temporal manner during embryonic development. There is a wealth of information on the deterministic embryonic development of Caenorhabditis elegans, but much less is known about embryonic development in nematodes from other taxa, especially at the molecular level. We are interested in insect pathogenic nematodes from the genus Steinernema as models of parasitism and symbiosis as well as a satellite model for evolution in comparison to C. elegans. To explore gene expression differences across taxa, we sequenced the transcriptomes of single embryos of two Steinernema species and two Caenorhabditis species at 11 stages during embryonic development and found several interesting features...
October 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29048250/inhibition-of-abeta-proteotoxicity-by-paeoniflorin-in-i-caenorhabditis-elegans-i-through-regulation-of-oxidative-and-heat-shock-stress-responses
#8
Liping Ai, Fan Yang, Jie Song, Yan Chen, Lingyun Xiao, Qiangqiang Wang, Liangyi Wang, Haifeng Li, Tao Lei, Zebo Huang
Alzheimer's disease (AD) is a common form of dementia and amyloid-β peptide (Aβ) aggregation is considered to be one of its main causes. Paeoniflorin has been previously shown to attenuate cognitive damage inflicted by exogenous Aβ protein. Using transgenic <i>Caenorhabditis elegans</i> models expressing human Aβ1-42, we demonstrate here that paeoniflorin can delay progressive paralysis caused by endogenous Aβ expression and reduce the amount of toxic Aβ oligomers in vivo although it has no effect on Aβ aggregation <i>in vitro</i>...
October 19, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/29047458/recordings-of-caenorhabditis-elegans-locomotor-behaviour-following-targeted-ablation-of-single-motorneurons
#9
Yee Lian Chew, Denise S Walker, Emma K Towlson, Petra E Vértes, Gang Yan, Albert-László Barabási, William R Schafer
Lesioning studies have provided important insight into the functions of brain regions in humans and other animals. In the nematode Caenorhabditis elegans, with a small nervous system of 302 identified neurons, it is possible to generate lesions with single cell resolution and infer the roles of individual neurons in behaviour. Here we present a dataset of ~300 video recordings representing the locomotor behaviour of animals carrying single-cell ablations of 5 different motorneurons. Each file includes a raw video of approximately 27,000 frames; each frame has also been segmented to yield the position, contour, and body curvature of the tracked animal...
October 19, 2017: Scientific Data
https://www.readbyqxmd.com/read/29046707/comparative-cytogenetic-analysis-of-marine-palaemon-species-reveals-a-x1x1x2x2-x1x2y-sex-chromosome-system-in-palaemon-elegans
#10
Zeltia Torrecilla, Andrés Martínez-Lage, Alejandra Perina, Enrique González-Ortegón, Ana M González-Tizón
BACKGROUND: The maintenance of species and the promotion of speciation are closely related to chromosomal rearrangements throughout evolution. Decapoda represents the most species-rich order among crustaceans and, despite its ecological and economic importance, little is known about decapod karyology. We aim at cytogenetically characterizing two sympatric prawn species. RESULTS: Analysis of mitotic metaphases and meiotic diakinesis of the common prawn Palaemon serratus and the rockpool prawn P...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/29046436/caenorhabditis-elegans-bub-3-and-san-1-mad3-spindle-assembly-checkpoint-components-are-required-for-genome-stability-in-response-to-treatment-with-ionizing-radiation
#11
Simone Bertolini, Bin Wang, Bettina Meier, Ye Hong, Anton Gartner
Relatively little is known about the crosstalk between the spindle assembly checkpoint and the DNA damage response, especially in multicellular organisms. We performed a Caenorhabditis elegans forward genetic screen to uncover new genes involved in the repair of DNA damage induced by ionizing radiation. We isolated a mutation, gt2000 which confers hypersensitivity to ionizing radiation and showed that gt2000 introduces a premature stop in bub-3 BUB-3 is a key component of the spindle assembly checkpoint. We provide evidence that BUB-3 acts during development and in the germline; irradiated bub-3(gt2000) larvae are developmentally retarded and form abnormal vulvae...
October 18, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29045848/single-molecule-sequencing-reveals-the-chromosome-scale-genomic-architecture-of-the-nematode-model-organism-pristionchus-pacificus
#12
Christian Rödelsperger, Jan M Meyer, Neel Prabh, Christa Lanz, Felix Bemm, Ralf J Sommer
The nematode Pristionchus pacificus is an established model for integrative evolutionary biology and comparative studies with Caenorhabditis elegans. While an existing genome draft facilitated the identification of several genes controlling various developmental processes, its high degree of fragmentation complicated virtually all genomic analyses. Here, we present a de novo genome assembly from single-molecule, long-read sequencing data consisting of 135 P. pacificus contigs. When combined with a genetic linkage map, 99% of the assembly could be ordered and oriented into six chromosomes...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045470/structural-and-functional-analysis-of-smo-1-the-sumo-homolog-in-caenorhabditis-elegans
#13
Parag Surana, Chandrakala M Gowda, Vasvi Tripathi, Limor Broday, Ranabir Das
SUMO proteins are important post-translational modifiers involved in multiple cellular pathways in eukaryotes, especially during the different developmental stages in multicellular organisms. The nematode C. elegans is a well known model system for studying metazoan development and has a single SUMO homolog, SMO-1. Interestingly, SMO-1 modification is linked to embryogenesis and development in the nematode. However, high-resolution information about SMO-1 and the mechanism of its conjugation is lacking. In this work, we report the high-resolution three dimensional structure of SMO-1 solved by NMR spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/29045391/network-control-principles-predict-neuron-function-in-the-caenorhabditis-elegans-connectome
#14
Gang Yan, Petra E Vértes, Emma K Towlson, Yee Lian Chew, Denise S Walker, William R Schafer, Albert-László Barabási
Recent studies on the controllability of complex systems offer a powerful mathematical framework to systematically explore the structure-function relationship in biological, social, and technological networks. Despite theoretical advances, we lack direct experimental proof of the validity of these widely used control principles. Here we fill this gap by applying a control framework to the connectome of the nematode Caenorhabditis elegans, allowing us to predict the involvement of each C. elegans neuron in locomotor behaviours...
October 18, 2017: Nature
https://www.readbyqxmd.com/read/29045128/radiation-dose-limits-for-bioanalytical-x-ray-fluorescence-microscopy
#15
Michael W M Jones, Dominic J Hare, Simon A James, Martin D de Jonge, Gawain McColl
Analytical approaches that preserve the endogenous state of the examined system are essential for the in vivo study of bioinorganics. X-ray fluorescence microscopy of biological samples can map elements in vivo at subcellular resolutions in tissue samples and multi-cellular organisms. However, X-ray irradiation induces modifications that accumulate with dose. Consequently, the utility of X-ray fluorescence microscopy is intrinsically limited by the radiation damage it causes and the degree to which it alters the target features of interest...
October 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29043382/cell-transfer-of-information-via-mir-loaded-exosomes-a-biophysical-approach
#16
C Potrich, L Lunelli, V Vaghi, L Pasquardini, C Pederzolli
A new communication route among cells was reported in recent years, via extracellular vesicles and their cargo. Exosomes in particular are attracting increasing interest as privileged mediators of this cell communication route. The exosome-mediated transfer of nucleic acids, especially of microRNAs, is particularly promising for their use both as biomarkers of pathologies and as a therapeutic tool. Here, a simplified model of interaction among cells, microRNAs and vesicles is studied using a biophysical approach...
October 17, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29043078/a-gap-that-divides
#17
REVIEW
Angika Basant, Michael Glotzer
Cytokinesis in metazoan cells is mediated by an actomyosin-based contractile ring that assembles in response to activation of the small GTPase RhoA. The guanine nucleotide exchange factor that activates RhoA during cytokinesis, ECT-2, is highly regulated. In most metazoan cells, with the notable exception of the early Caenorhabditis elegans embryo, RhoA activation and furrow ingression require the centralspindlin complex. This exception is due to the existence of a parallel pathway for RhoA activation in C...
2017: F1000Research
https://www.readbyqxmd.com/read/29042596/host-heterozygosity-and-genotype-rarity-affect-viral-dynamics-in-an-avian-subspecies-complex
#18
Justin R Eastwood, Raoul F H Ribot, Lee Ann Rollins, Katherine L Buchanan, Ken Walder, Andrew T D Bennett, Mathew L Berg
Genetic diversity at community, population and individual levels is thought to influence the spread of infectious disease. At the individual level, inbreeding and heterozygosity are associated with increased risk of infection and disease severity. Host genotype rarity may also reduce infection risk if pathogens are co-adapted to common or local hosts, but to date, no studies have investigated the relative importance of genotype rarity and heterozygosity for infection in a wild, sexually reproducing vertebrate...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042483/olfactory-experience-primes-the-heat-shock-transcription-factor-hsf-1-to-enhance-the-expression-of-molecular-chaperones-in-c-elegans
#19
Felicia K Ooi, Veena Prahlad
Learning, a process by which animals modify their behavior as a result of experience, enables organisms to synthesize information from their surroundings to acquire resources and avoid danger. We showed that a previous encounter with only the odor of pathogenic bacteria prepared Caenorhabditis elegans to survive exposure to the pathogen by increasing the heat shock factor 1 (HSF-1)-dependent expression of genes encoding molecular chaperones. Experience-mediated enhancement of chaperone gene expression required serotonin, which primed HSF-1 to enhance the expression of molecular chaperone genes by promoting its localization to RNA polymerase II-enriched nuclear loci, even before transcription occurred...
October 17, 2017: Science Signaling
https://www.readbyqxmd.com/read/29041955/pluripotent-nontumorigenic-multilineage-differentiating-stress-enduring-cells-muse-cells-a-seven-year-retrospective
#20
REVIEW
Samantha C Fisch, María L Gimeno, Julia D Phan, Ariel A Simerman, Daniel A Dumesic, Marcelo J Perone, Gregorio D Chazenbalk
Multilineage differentiating stress enduring (Muse) cells, discovered in the spring of 2010 at Tohoku University in Sendai, Japan, were quickly recognized by scientists as a possible source of pluripotent cells naturally present within mesenchymal tissues. Muse cells normally exist in a quiescent state, singularly activated by severe cellular stress in vitro and in vivo. Muse cells have the capacity for self-renewal while maintaining pluripotent cell characteristics indicated by the expression of pluripotent stem cell markers...
October 18, 2017: Stem Cell Research & Therapy
keyword
keyword
26130
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"