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D R Griffin, E J Dickenson, J O'Donnell, R Agricola, T Awan, M Beck, J C Clohisy, H P Dijkstra, E Falvey, M Gimpel, R S Hinman, P Hölmich, A Kassarjian, H D Martin, R Martin, R C Mather, M J Philippon, M P Reiman, A Takla, K Thorborg, S Walker, A Weir, K L Bennell
The 2016 Warwick Agreement on femoroacetabular impingement (FAI) syndrome was convened to build an international, multidisciplinary consensus on the diagnosis and management of patients with FAI syndrome. 22 panel members and 1 patient from 9 countries and 5 different specialties participated in a 1-day consensus meeting on 29 June 2016. Prior to the meeting, 6 questions were agreed on, and recent relevant systematic reviews and seminal literature were circulated. Panel members gave presentations on the topics of the agreed questions at Sports Hip 2016, an open meeting held in the UK on 27-29 June...
October 2016: British Journal of Sports Medicine
Dana Ulanova, Shigeru Kitani, Eiichiro Fukusaki, Takuya Nihira
The SAV3339 (SdrA) protein of Streptomyces avermitilis, a member of the DeoR family of regulators, was assessed to determine its in vivo function by gene knockdown through the use of cis-encoded noncoding RNA and knockout of the sdrA gene. These analyses revealed that SdrA represents another class of Streptomyces regulator that controls morphological development and antibiotic production.
December 2013: Applied and Environmental Microbiology
Claudio Poggio, Marco Chiesa, Andrea Scribante, Jenia Mekler, Marco Colombo
OBJECTIVES: The purpose of this in vitro study was to evaluate the microleakage in "deep" Class II composite restorations with gingival cavosurface margin below the CEJ (cemento-enamel junction) and restored with different techniques. STUDY DESIGN: Fifty human teeth were used. In each tooth two standardized Class II slot cavities (on mesial and on distal surfaces) were prepared: the buccolingual extension of the cavities was 4 mm; the gingival wall was located in dentin/cementum (2 mm beyond the CEJ)...
September 2013: Medicina Oral, Patología Oral y Cirugía Bucal
Malcolm Stratford, Andrew Plumridge, Mike W Pleasants, Michaela Novodvorska, Charles A G Baker-Glenn, Gerald Pattenden, David B Archer
Moulds are able to cause spoilage in preserved foods through degradation of the preservatives using the Pad-decarboxylation system. This causes, for example, decarboxylation of the preservative sorbic acid to 1,3-pentadiene, a volatile compound with a kerosene-like odour. Neither the natural role of this system nor the range of potential substrates has yet been reported. The Pad-decarboxylation system, encoded by a gene cluster in germinating spores of the mould Aspergillus niger, involves activity by two decarboxylases, PadA1 and OhbA1, and a regulator, SdrA, acting pleiotropically on sorbic acid and cinnamic acid...
July 16, 2012: International Journal of Food Microbiology
Pascal Czasch, Nicoleta Ilie
OBJECTIVES: The aim of our study was to measure and compare degree of conversion (DC) as well as micro- (indentation modulus, E; Vickers hardness, HV) and macromechanical properties (flexural strength, σ; flexural modulus, E (flexural)) of two recently launched bulk fill resin-based composites (RBCs): Surefil® SDR™ flow (SF) and Venus® bulk fill (VB). MATERIALS AND METHODS: DC (n = 6) was investigated by Fourier transform infrared spectroscopy (FTIR) in clinical relevant filling depths (0...
January 2013: Clinical Oral Investigations
Agostino Giovannetti, Cecilia Goracci, Alessandro Vichi, Nicoletta Chieffi, Antonella Polimeni, Marco Ferrari
OBJECTIVES: To assess by means of push-out test the post retentive potential of a new flowable resin composite with low polymerization stress. METHODS: SureFil(®) SDR™ (Dentsply, S) was compared with the resin cement Calibra (Dentsply, C). S and C were used in combination with the adhesive XP Bond (Dentsply, XPB). In C group the Self-Cure Activator was mixed with XPB. The following types of fibre posts were luted into 30 extracted premolars: Radix Fiber Post (Dentsply, R), DT Light Post (RTD, LP), ER Dentin Post (Komet, ER), DT Light SL (VDW, SL), FibreKleer (Jeneric Pentron, F)...
April 2012: Journal of Dentistry
Nicoleta Ilie, Reinhard Hickel
OBJECTIVE: Monomer development for a reduced shrinkage of composite materials still challenges the modern research. The purpose of this study was to analyse the shrinkage behavior of an innovative composite material for dental restorations based on a resin system that is claimed to control polymerization kinetics having incorporated a photoactive group within the resin. METHODS: Shrinkage stress development within the first 300s after photoinitiation, gel point as well as micro-mechanical properties (Vickers hardness HV, modulus of elasticity E, creep Cr and elastic-plastic indentation work W(e)/W(tot)) were evaluated (n = 10)...
April 2011: Dental Materials: Official Publication of the Academy of Dental Materials
Andrew Plumridge, Petter Melin, Malcolm Stratford, Michaela Novodvorska, Lee Shunburne, Paul S Dyer, Johannes A Roubos, Hildegard Menke, Jacques Stark, Hein Stam, David B Archer
The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadienoic acid) to 1,3-pentadiene. It is now shown that this decarboxylation process also requires the activity of a putative 4-hydroxybenzoic acid (3-octaprenyl-4-hydroxybenzoic acid) decarboxylase, encoded by a gene termed ohbA1, and a putative transcription factor, sorbic acid decarboxylase regulator, encoded by sdrA...
August 2010: Fungal Genetics and Biology: FG & B
Rebekka Heitmar, Andrew D Blann, Robert P Cubbidge, Gregory Y H Lip, Doina Gherghel
PURPOSE: To establish an alternative method, sequential and diameter response analysis (SDRA), to determine dynamic retinal vessel responses and their time course in serial stimulation compared with the established method of averaged diameter responses and standard static assessment. METHODS: SDRA focuses on individual time and diameter responses, taking into account the fluctuation in baseline diameter, providing improved insight into reaction patterns when compared with established methods as delivered by retinal vessel analyzer (RVA) software...
November 2010: Investigative Ophthalmology & Visual Science
Lavanya Babujee, Virginie Wurtz, Changle Ma, Franziska Lueder, Pradeep Soni, Alain van Dorsselaer, Sigrun Reumann
Leaf peroxisomes are fragile, low-abundance plant cell organelles that are difficult to isolate from one of the few plant species whose nuclear genome has been sequenced. Leaf peroxisomes were enriched at high purity from spinach (Spinacia oleracea) and approximately 100 protein spots identified from 2-dimensional gels by a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and de novo sequencing. In addition to the predominant enzymes involved in photorespiration and detoxification, several minor enzymes were detected, underscoring the high sensitivity of the protein identification...
March 2010: Journal of Experimental Botany
Andrew A G Wiszniewski, Wenxu Zhou, Steven M Smith, John D Bussell
Indole-3-butyric acid (IBA) and 2,4-dichlorophenoxybutyric acid (2,4-DB) are metabolised by peroxisomal beta-oxidation to active auxins that inhibit root growth. We screened Arabidopsis mutants for resistance to IBA and 2,4-DB and identified two new 2,4-DB resistant mutants. The mutant genes encode a putative oxidoreductase (SDRa) and a putative acyl-activating enzyme (AAE18). Both proteins are localised to peroxisomes. SDRa is coexpressed with core beta-oxidation genes, but germination, seedling growth and the fatty acid profile of sdra seedlings are indistinguishable from wild type...
March 2009: Plant Molecular Biology
E Meaudre, Y Asencio, A Montcriol, C Martinaud, B Graffin, B Palmier, P Goutorbe
We report a case of severe leptospirosis complicated with a multiple organ failure syndrome. A 62-year-old patient presented a picture associating fever, asthenia and myalgias, particularly intense on the calves. The assessment showed acute renal failure, hyperbilirubinemia, severe rhabdomyolysis and thrombocytopenia. Although initial management associating amoxicilline and continuous veino-venous hemodiafiltration, evolution was unfavourable, with SDRA and increase of hyperbilirubinemia. Administration of a bolus of 500 mg of methylprednisolone, associated with intravenous immunoglobulin (0,4 g/kg per day during five days), led to a rapid clinical and biological improvement...
February 2008: Annales Françaises D'anesthèsie et de Rèanimation
Patrice Bruscella, Corinne Appia-Ayme, Gloria Levicán, Jeanine Ratouchniak, Eugenia Jedlicki, David S Holmes, Violaine Bonnefoy
Three strains of the strict acidophilic chemolithoautotrophic Acidithiobacillus ferrooxidans, including the type strain ATCC 23270, contain a petIIABC gene cluster that encodes the three proteins, cytochrome c1, cytochrome b and a Rieske protein, that constitute a bc1 electron-transfer complex. RT-PCR and Northern blotting show that the petIIABC cluster is co-transcribed with cycA, encoding a cytochrome c belonging to the c4 family, sdrA, encoding a putative short-chain dehydrogenase, and hip, encoding a high potential iron-sulfur protein, suggesting that the six genes constitute an operon, termed the petII operon...
January 2007: Microbiology
G I Kravatskaia, G K Frank, V Iu Makeev, N G Esipova
The regions of initiation of replication of some bacterial genomes were studied by the method of Fourier matrix analysis. A generalized spectral portrait of the primary structures of E. coli-like regions of initiation of replication in bacteria was obtained, which reflects the features of their structural and functional organization. It contains well-pronounced peaks that correspond to the periods T = 2, 11, 17, 27, 86-105 of nucleotides. The peaks corresponding to T = 9, 13, 14, 18, 19, 33-35, 45-47, 74-85, 106-110 are less pronounced...
July 2002: Biofizika
T Kogoma, K von Meyenburg
The sdrA224 mutants of Escherichia coli K-12, capable of continued DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E. coli chromosome replication, can be deleted from the chromosome of sdrA mutants without loss of viability. The results suggest the presence of a second, normally repressed, initiation system for chromosome replication alternative to the 'normal' dnaA+ oriC+-dependent initiation mechanism...
1983: EMBO Journal
L González Zambrano, E San Román, A O Gallesio, A F Prados, G J Principe
The aim of the present study was to evaluate the hemodynamic and respiratory variations in patients with acute respiratory distress syndrome (ARDS) under two conditions: volume controlled ventilation (VCV) and pressure controlled inverse ratio ventilation (PCIRV). Seventeen patients with ARDS and the following criteria were included: lung injury score > 2.5 and peak inspiratory pressure > or = 40 cm H2O under VCV, constant flow and I/E ratio of 1/2. Measurements were obtained in VCV and after one hour in PCIRV with I/E ratio 2/1 using a similar PEEP value than VCV...
1997: Medicina
L Marruecos-Sant, M Pérez-Márquez, A J Betbesé, G Rialp, J Molet, A Net
BACKGROUND: To analyse extracranial complications and basic variables in head-injury patients, such as Glasgow coma score (GCS), intracranial pressure (ICP) and cranial computerized tomography (CT), in relation to the outcome of these patients. PATIENTS AND METHODS: 64 consecutive patients (47 males and 17 females) with head injury, admitted from January 1992 to May 1994, were studied in this prospective study. Mean age was 37 +/- 18 years. Overall mortality was 23% (15/64)...
October 5, 1996: Medicina Clínica
E Oliva Torras, M Subirana Casacuberta, M P Sebastià, C Jover Sancho, N Solà Solé
Offering a specific integral attention to patients with SDRA in prone decubitus positions makes us establish a performance plan with the aim to know the problems derived from the change in position, the time staying in prone decubitus and to standardize a care plan. We review the clinic records of the patients admitted in our unit from March '93 to March '95 who were positioned in prone decubitus. Taking as a base the nursing care model of V. Henderson and the taxonomy of NANDA, we analyse the needs which have been altered, and determine the nursing diagnosis, complications and most frequent interdependent problems establishing the aim to accomplish, planning the performance and rationalization...
October 1995: Enfermería Intensiva
B de Massy, O Fayet, T Kogoma
In stable DNA replication (sdrA/rnh) mutants of Escherichia coli, initiation of rounds of DNA replication occurs in the absence of the normal origin of replication, oriC. To determine whether or not the initiation occurs at a fixed site(s) on the chromosome in sdrA mutants, the DNA from exponentially growing sdrA mutant cells with or without the oriC site (delta oriC) was analyzed for the relative copy numbers of various genes along the chromosome. The results suggest that there are at least four fixed sites or regions of the sdrA delta oriC chromosome from which DNA replication can be initiated in the absence of the oriC sequence...
September 15, 1984: Journal of Molecular Biology
T Ogawa, G G Pickett, T Kogoma, A Kornberg
Escherichia coli rnh mutants defective in RNase H activity display the features of previously described sdrA (stable DNA replication) and dasF (dnaA suppressor) mutants: (i) sustained DNA replication in the absence of protein synthesis, (ii) lack of requirement for dnaA protein and the origin of replication (oriC), and (iii) sensitivity of growth to a rich medium. Both the sdrA mutants (selected for continued DNA replication in the absence of protein synthesis) and the dasF mutants (selected as dnaA suppressors) are defective in RNase H activity, measured in vitro...
February 1984: Proceedings of the National Academy of Sciences of the United States of America
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