keyword
MENU ▼
Read by QxMD icon Read
search

Free light chains

keyword
https://www.readbyqxmd.com/read/29142197/proteotoxicity-in-cardiac-amyloidosis-amyloidogenic-light-chains-affect-the-levels-of-intracellular-proteins-in-human-heart-cells
#1
Esther Imperlini, Massimiliano Gnecchi, Paola Rognoni, Eduard Sabidò, Maria Chiara Ciuffreda, Giovanni Palladini, Guadalupe Espadas, Francesco Mattia Mancuso, Margherita Bozzola, Giuseppe Malpasso, Veronica Valentini, Giuseppina Palladini, Stefania Orrù, Giovanni Ferraro, Paolo Milani, Stefano Perlini, Francesco Salvatore, Giampaolo Merlini, Francesca Lavatelli
AL amyloidosis is characterized by widespread deposition of immunoglobulin light chains (LCs) as amyloid fibrils. Cardiac involvement is frequent and leads to life-threatening cardiomyopathy. Besides the tissue alteration caused by fibrils, clinical and experimental evidence indicates that cardiac damage is also caused by proteotoxicity of prefibrillar amyloidogenic species. As in other amyloidoses, the damage mechanisms at cellular level are complex and largely undefined. We have characterized the molecular changes in primary human cardiac fibroblasts (hCFs) exposed in vitro to soluble amyloidogenic cardiotoxic LCs from AL cardiomyopathy patients...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137262/disease-spectrum-of-abnormal-serum-free-light-chain-ratio-and-its-diagnostic-significance
#2
Bin Xu, Yi Tang, Jianfeng Zhou, Peiling Zhang, Huijun Li
Objective: To analyze the spectrum of abnormal serum free light chain ratio (sFLC κ/λ ratio), and to redefine the range of sFLC κ/λ ratio, so as to achieve hierarchical diagnosis of diseases with abnormal sFLC κ/λ ratio, resulting in the increased sensitivity and specificity in the diagnosis of monoclonal plasma diseases. Methods: Enrolled 1,340 patients with abnormal sFLC κ/λ ratio (<0.26 or >1.65) were grouped: (1) group A: malignant plasma diseases; (2) group B: monoclonal gammopathies of undetermined significance (MGUS); (3) group C: reactive plasma diseases...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136724/-expert-consensus-for-the-diagnosis-and-treatment-of-patients-with-renal-impairment-of-multiple-myeloma
#3
(no author information available yet)
Renal impairment (RI) is a common complication of multiple myeloma (MM), which is presented as chronic kidney disease (CKD) or acute kidney injury (AKI). The typical pathological feature is cast nephropathy. Presently international system staging (ISS) is used in evaluating MM. Although the classic Durie-Salmon staging system could be still used in clinical practice, it may miss out some patients with renal impairment. For evaluations of RI in MM patients with CKD, it's recommended to assess the estimated glomerular filtration rate (eGFR) by creatinine based formula CKD-epidemiology collaboration (EPI) or modification of diet in renal disease(MDRD) and to stage the renal injuries according to 2013 Kidney Disease Improving Global Outcomes (KDIGO) CKD guidelines...
November 1, 2017: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/29135289/-what-should-we-know-about-cardiac-amyloidosis-from-clinical-signs-to-treatment
#4
Dóra Földeák, Attila Nemes, Anita Kalapos, Péter Domsik, Árpád Kormányos, László Krenács, Enikő Bagdi, Zita Borbényi
Systemic amyloidosis is a rare disease, in which the heart involvement is rather frequent and determines survival remarkably. Regarding the disease and organ involvement, new diagnostic procedures help to establish the diagnosis and to start the adequate treatment as soon as possible. Cardiac involvement is more likely to be characterised by monoclonal immunglobulin free light chain (AL amyloidosis) type and transthyretin type. In case of AL amyloidosis, heart involvement can lead to serious consequences. Biomarker assessments for cardiac function are important to determine disease severity at the beginning and to measure response to the treatment...
November 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29135062/organocatalyzed-photo-controlled-radical-polymerization-of-semi-fluorinated-meth-acrylates-driven-by-visible-light
#5
Mao Chen, Honghong Gong, Yucheng Zhao, Xianwang Shen, Jun Lin
Fluorinated polymers are important materials that are widely used in many areas. Herein, we report the development of a metal-free photo-controlled radical polymerization of semi-fluorinated (meth)acrylates with a new visible light absorbing organocatalyst. This method allows the production of a variety of semi-fluorinated polymers with narrow molar weight distributions from semi-fluorinated trithiocarbonates or perfluoroalkyl iodide. The high performance of "ON/OFF" control and chain extension experiments further demonstrate the utility and reliability of this method...
November 14, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29133141/abdominal-fat-pad-excisional-biopsy-for-the-diagnosis-and-typing-of-systemic-amyloidosis
#6
Yessica Garcia, A Bernard Collins, James R Stone
In the past, the diagnosis and typing of amyloidosis often required an invasive biopsy of an internal organ, such as the heart or kidneys. Abdominal fat pad excisional biopsy (FPEB) offers a less invasive approach, but the sensitivity of this technique has been unclear. To determine the sensitivity of FPEB for immunoglobulin light chain (AL) and transthyretin (ATTR) amyloidosis, we performed a retrospective clinicopathologic analysis of 97 patients who had undergone FPEB, of which 16 were positive for amyloid...
November 10, 2017: Human Pathology
https://www.readbyqxmd.com/read/29121956/regional-differences-in-prognostic-value-of-cardiac-valve-plane-displacement-in-systemic-light-chain-amyloidosis
#7
Marco M Ochs, Thomas Fritz, Nisha Arenja, Johannes Riffel, Florian Andre, Derliz Mereles, Fabian Aus dem Siepen, Ute Hegenbart, Stefan Schönland, Hugo A Katus, Matthias G W Friedrich, Sebastian J Buss
BACKGROUND: To compare the prognostic value of cardiac valve plane displacement (CVPD) on various locations in cardiac light chain (AL) amyloidosis. METHODS: Consecutive patients with biopsy-proven cardiac involvement in AL amyloidosis who had undergone cardiovascular magnetic resonance (CMR) between 2005 and 2014 in our institution, were retrospectively identified and data analyzed. The primary combined endpoint was all-cause mortality or heart transplantation...
November 9, 2017: Journal of Cardiovascular Magnetic Resonance
https://www.readbyqxmd.com/read/29114035/an-ultra-stable-single-chain-insulin-analog-resists-thermal-inactivation-and-exhibits-biological-signaling-duration-equivalent-to-the-native-protein
#8
Michael D Glidden, Khadijah Aldabbagh, Nelson B Phillips, Kelley Carr, Yen-Shan Chen, Jonathan Whittaker, Manijeh Phillips, Nalinda P Wickramasinghe, Nischay Rege, Mamuni Swain, Yi Peng, Yanwu Yang, Michael C Lawrence, Vivien C Yee, Faramarz Ismail-Beigi, Michael A Weiss
Thermal degradation of insulin complicates its delivery and use. Previous efforts to engineer ultra-stable analogs were confounded by prolonged cellular signaling in vivo, complicating mealtime therapy and of unclear safety. We therefore sought an ultra-stable analog whose potency and duration of action on intravenous bolus injection in diabetic rats are indistinguishable from wild-type (WT) insulin. Here, we describe the structure, function and stability of such an analog: a 57-residue single-chain insulin (SCI) with multiple acidic substitutions...
November 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29112748/intermittent-lighting-improves-resilience-of-broilers-during-the-peak-phase-of-sub-clinical-necrotic-enteritis-infection
#9
I Rodrigues, B Svihus, M R Bedford, R Gous, M Choct
Necrotic enteritis, either in its clinical or sub-clinical form is known to cause massive economic losses in the broiler chicken industry. Currently, the use of in-feed antibiotics as growth promoters is discouraged. Therefore, mechanisms to control NE through diet include reduction of digesta viscosity, promotion of lower pH in the gastrointestinal tract (GIT) through acidification of feed, production of short-chain fatty acids and overall stimulation of beneficial bacteria growth. Intermittent lighting programs increase feed retention in the crop and reduce pH in the foregut compartments in comparison with standard commercial lighting programs and therefore may be a valuable, yet underexploited, barrier to prevent the invasion of the GIT by pathogens...
November 3, 2017: Poultry Science
https://www.readbyqxmd.com/read/29111123/prognostic-and-added-value-of-two-dimensional-global-longitudinal-strain-for-prediction-of-survival-in-patients-with-light-chain-amyloidosis-undergoing-autologous-hematopoietic-cell%C3%A2-transplantation
#10
Shawn C Pun, Heather J Landau, Elyn R Riedel, Jonathan Jordan, Anthony F Yu, Hani Hassoun, Carol L Chen, Richard M Steingart, Jennifer E Liu
BACKGROUND: Autologous hematopoietic cell transplantation (HCT) is a first-line therapy for prolonging survival in patients with light-chain (AL) amyloidosis. Cardiac involvement is the most important determinant of survival. However, patients with advanced cardiac involvement have often been excluded from HCT because of high risk for transplantation-related mortality and poor overall survival. Whether baseline left ventricular global longitudinal strain (GLS) can provide additional risk stratification and predict survival after HCT in this high-risk population remains unclear...
October 27, 2017: Journal of the American Society of Echocardiography
https://www.readbyqxmd.com/read/29101935/the-association-of-serum-free-light-chains-with-mortality-and-progression-to-end-stage-renal-disease-in-chronic-kidney-disease-systematic-review-and-individual-patient-data-meta-analysis
#11
Simon D S Fraser, Anthony Fenton, Scott Harris, Adam Shardlow, Sophie Liabeuf, Ziad A Massy, Anne Burmeister, Colin A Hutchison, Martin Landray, Jonathan Emberson, Phil Kalra, James P Ritchie, Paul Cockwell, Maarten W Taal
OBJECTIVE: To clarify the associations between polyclonal serum free light chain (sFLC) levels and adverse outcomes in patients with chronic kidney disease (CKD) by conducting a systematic review and individual patient data meta-analyses. PATIENTS AND METHODS: On December 28, 2016, we searched 4 databases (MEDLINE, Embase, CINAHL, and PubMed) and conference proceedings for studies presenting independent analyses of associations between sFLC levels and mortality or progression to end-stage renal disease (ESRD) in patients with CKD...
November 2017: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/29093202/isotype-determination-of-rodent-derived-monoclonal-antibodies-using-sandwich-elisa
#12
Frances Weis-Garcia, Robert H Carnahan
For research groups needing to isotype a significant number of antibodies on a fairly regular basis, the protocol outlined here provides a cost-effective option. In this simple sandwich ELISA, the monoclonal antibody is first captured with antibodies that discriminate between heavy-chain isotypes and then is detected with antibodies specific for each light chain. This combination ensures that free light chain secreted by hybridomas does not yield a false-positive result, because in a true positive both the capture and detecting antibodies bind the monoclonal antibody...
November 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29089806/short-course-lenalidomide-plus-low-dose-dexamethasone-in-the-treatment-of-newly-diagnosed-multiple-myeloma-a-single-centre-pragmatic-study
#13
W M Jose, K Pavithran, T S Ganesan
PURPOSE: We assessed response to treatment, toxicity, time to progression, progression-free survival, and overall survival in patients newly diagnosed with multiple myeloma who were ineligible for or unwilling to undergo transplantation and who were treated with a combination of lenalidomide and low-dose dexamethasone for a fixed 6 cycles in a resource-constrained environment. METHODS: This pragmatic study, conducted in a single tertiary cancer centre in South India, enrolled patients from May 2009 till April 2011...
October 2017: Current Oncology
https://www.readbyqxmd.com/read/29076532/the-polypeptide-biophysics-of-proline-alanine-rich-sequences-pas-recombinant-biopolymers-with-peg-like-properties
#14
Joscha Breibeck, Arne Skerra
PAS polypeptides comprise long repetitive sequences of the small L-amino acids proline, alanine and/or serine that were developed to expand the hydrodynamic volume of conjugated pharmaceuticals and prolong their plasma half-life by retarding kidney filtration. Here, we have characterized the polymer properties both of the free polypeptides and in fusion with the biopharmaceutical IL-1Ra. Data from size exclusion chromatography, dynamic light scattering, circular dichroism spectroscopy and quantification of hydrodynamic and polar properties demonstrate that the biosynthetic PAS polypeptides exhibit random coil behavior in aqueous solution astonishingly similar to the chemical polymer poly-ethylene glycol (PEG)...
October 27, 2017: Biopolymers
https://www.readbyqxmd.com/read/29070795/ecobody-technology-rapid-monoclonal-antibody-screening-method-from-single-b-cells-using-cell-free-protein-synthesis-for-antigen-binding-fragment-formation
#15
Teruyo Ojima-Kato, Satomi Nagai, Hideo Nakano
We report a rapid and cost-effective monoclonal antibody screening method from single animal B cells using reverse transcription (RT)-PCR and Escherichia coli cell-free protein synthesis (CFPS), which allows evaluation of antibodies within 2 working days. This process is named "Ecobody technology". The method includes strategies to isolate B cells that specifically bind an antigen from the peripheral blood of immunised animals, and single-cell RT-PCR to generate DNA fragments of the VH and VL genes, followed by CFPS for production of fragments of antigen binding (Fab)...
October 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29066226/cast-nephropathy-and-deceptively-low-absolute-serum-free-light-chain-levels-resolution-of-a-challenging-case-and-systematic-review-of-the-literature
#16
Michael P Rassner, Maximilian Seidl, Ulrich Salzer, S Vincent Rajkumar, Thomas Epting, Ralph Wäsch, Elke Neumann-Haefelin, Monika Engelhardt
No abstract text is available yet for this article.
October 3, 2017: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/29052436/prognostic-impact-of-immunoparesis-at-diagnosis-and-after-treatment-onset-in-patients-with-light-chain-amyloidosis
#17
Luis Gerardo Rodríguez-Lobato, Carlos Fernández de Larrea, Maria Teresa Cibeira, Natalia Tovar, Ignacio Isola, Juan I Aróstegui, Laura Rosiñol, Tania Díaz, Ester Lozano, Jordi Yagüe, Joan Bladé
OBJECTIVES: Immunoparesis (IP) is a risk factor associated with an unfavourable outcome in several plasma cell disorders. It has been suggested that its presence in light-chain (AL) amyloidosis could be associated with worse prognosis. However, the relevance of IP after treatment has not been evaluated to date. The aim of this study was to determine the prognostic impact of IP at diagnosis and one year after treatment onset in patients with AL amyloidosis. METHODS: The clinical records of 69 patients with AL amyloidosis treated at a single institution from January 2006 to January 2016 were included in the study...
October 20, 2017: Amyloid: the International Journal of Experimental and Clinical Investigation
https://www.readbyqxmd.com/read/29052230/omecamtiv-mercabil-and-blebbistatin-modulate-cardiac-contractility-by-perturbing-the-regulatory-state-of-the-myosin-filament
#18
Thomas Kampourakis, Xuemeng Zhang, Yin-Biao Sun, Malcolm Irving
Contraction of heart muscle is triggered by a transient rise in intracellular free calcium concentration linked to a change in the structure of the actin-containing thin filaments that allows the head or motor domains of myosin from the thick filaments to bind to them and induce filament sliding. It is becoming increasingly clear that cardiac contractility is also regulated through structural changes in the thick filaments, although the molecular mechanisms underlying thick filament regulation are still relatively poorly understood...
October 20, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29051672/photoinduced-organocatalyzed-atom-transfer-radical-polymerization-using-continuous-flow
#19
Bonnie L Ramsey, Ryan M Pearson, Logan R Beck, Garret M Miyake
Organocatalyzed atom transfer radical polymerization (O-ATRP) has emerged as a metal-free variant of historically transition-metal reliant atom transfer radical polymerization. Strongly reducing organic photoredox catalysts have proven capable of mediating O-ATRP. To date, operation of photoinduced O-ATRP has been demonstrated in batch reactions. However, continuous flow approaches can provide efficient irradiation reaction conditions and thus enable increased polymerization performance. Herein, the adaptation of O-ATRP to a continuous flow approach has been performed with multiple visible-light absorbing photoredox catalysts...
April 11, 2017: Macromolecules
https://www.readbyqxmd.com/read/29044686/thiol-ene-clickable-gelatin-a-platform-bioink-for-multiple-3d-biofabrication-technologies
#20
Sarah Bertlein, Gabriella Brown, Khoon S Lim, Tomasz Jungst, Thomas Boeck, Torsten Blunk, Joerg Tessmar, Gary J Hooper, Tim B F Woodfield, Juergen Groll
Bioprinting can be defined as the art of combining materials and cells to fabricate designed, hierarchical 3D hybrid constructs. Suitable materials, so called bioinks, have to comply with challenging rheological processing demands and rapidly form a stable hydrogel postprinting in a cytocompatible manner. Gelatin is often adopted for this purpose, usually modified with (meth-)acryloyl functionalities for postfabrication curing by free radical photopolymerization, resulting in a hydrogel that is cross-linked via nondegradable polymer chains of uncontrolled length...
October 17, 2017: Advanced Materials
keyword
keyword
26106
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"