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https://www.readbyqxmd.com/read/28439480/development-of-microsatellite-markers-based-on-expressed-sequence-tags-in-asparagus-cochinchinensis-asparagaceae
#1
Bo-Yun Kim, Han-Sol Park, Jung-Hoon Lee, Myounghai Kwak, Young-Dong Kim
PREMISE OF THE STUDY: Transcriptome-derived simple sequence repeat (SSR) markers were developed in Asparagus cochinchinensis (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts. METHODS AND RESULTS: Based on transcriptome data of A. cochinchinensis, 96 primer pairs with two to seven alleles per locus were selected for initial validation; of those, 27 primer pairs amplified across all samples, resulting in 15 polymorphic and 12 monomorphic microsatellite markers...
April 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28436323/new-germplasms-of-the-culinary-medicinal-button-mushroom-agaricus-bisporus-agaricomycetes-two-wild-strains-from-the-tibetan-plateau-china
#2
Bin-Bin Li, Si-Ping Jiang, Ai-Guo Xu, Phurbu Dorji, Wen-Jing Wang, Xiao-Liang Wang, Tie-Zheng Wei, Zu-Tang Zhang, Yi-Jian Yao
Agaricus bisporus is one of the most important commercially cultivated culinary-medicinal mushrooms worldwide. In China, most of the cultivated strains of the fungus were introduced from other countries and cultivated in the eastern provinces. In this study, 2 wild strains of A. bisporus, 2091 and 2094, isolated from fresh specimens collected from the Tibetan Plateau, were domesticated and cultivated alongside a commercial hybrid strain, As2796, in Lhasa, China, for comparison in order to provide new germplasms for cultivation...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436321/screening-of-bioactive-compounds-of-medicinal-mushrooms-collected-on-tunisian-territory
#3
Ayda Khadhri, Chedia Aouadhi, Samira Aschi-Smiti
This study is, to our knowledge, the first to investigate the pharmacological importance of wild Tunisian mushrooms. Ethanolic extracts of 5 Tunisian mushrooms-Phellinus torulosus, Fomes fomentarius, Trametes versicolor, Pisolithus albus, and Fomitopsis pinicola-were collected from the Kroumirie Region (North Tunisia). The dry basidomes of mushrooms were extracted using ethanol and evaluated for total polyphenol, flavonoid, flavonol, tannin, proanthocyanidin, and anthocyanin content. In addition, their antioxidant activities were determined using 3 assays (testing 2,2-diphenyl-1-picrylhydrazyl [DPPH] radical scavenging, the reducing power of iron, and the iron-chelating power)...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28416672/bisulfite-converted-duplexes-for-the-strand-specific-detection-and-quantification-of-rare-mutations
#4
Austin K Mattox, Yuxuan Wang, Simeon Springer, Joshua D Cohen, Srinivasan Yegnasubramanian, William G Nelson, Kenneth W Kinzler, Bert Vogelstein, Nickolas Papadopoulos
The identification of mutations that are present at low frequencies in clinical samples is an essential component of precision medicine. The development of molecular barcoding for next-generation sequencing has greatly enhanced the sensitivity of detecting such mutations by massively parallel sequencing. However, further improvements in specificity would be useful for a variety of applications. We herein describe a technology (BiSeqS) that can increase the specificity of sequencing by at least two orders of magnitude over and above that achieved with molecular barcoding and can be applied to any massively parallel sequencing instrument...
April 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28416198/engineering-human-renal-epithelial-cells-for-transplantation-in-regenerative-medicine
#5
Vita Manzoli, David C Colter, Sridevi Dhanaraj, Alessia Fornoni, Camillo Ricordi, Antonello Pileggi, Alice A Tomei
Cellular transplantation may treat several human diseases by replacing damaged cells and/or providing a local source of trophic factors promoting regeneration. We utilized human renal epithelial cells (hRECs) isolated from cadaveric donors as a cell model. For efficacious implementation of hRECs for treatment of kidney diseases, we evaluated a novel encapsulation strategy for immunoisolation of hRECs and lentiviral transduction of the Green Fluorescent Protein (GFP) as model gene for genetic engineering of hRECs to secrete desired trophic factors...
April 13, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28409502/the-role-of-wildlife-wild-birds-in-the-global-transmission-of-antimicrobial-resistance-genes
#6
REVIEW
Jing Wang, Zhen-Bao Ma, Zhen-Ling Zeng, Xue-Wen Yang, Ying Huang, Jian-Hua Liu
Antimicrobial resistance is an urgent global health challenge in human and veterinary medicine. Wild animals are not directly exposed to clinically relevant antibiotics; however, antibacterial resistance in wild animals has been increasingly reported worldwide in parallel to the situation in human and veterinary medicine. This underlies the complexity of bacterial resistance in wild animals and the possible interspecies transmission between humans, domestic animals, the environment, and wildlife. This review summarizes the current data on expanded-spectrum β-lactamase (ESBL), AmpC β-lactamase, carbapenemase, and colistin resistance genes in Enterobacteriaceae isolates of wildlife origin...
March 18, 2017: Zoological Research
https://www.readbyqxmd.com/read/28408230/discovery-of-lrrk2-inhibitors-by-using-an-ensemble-of-virtual-screening-methods
#7
Emanuela Gancia, Marcel De Groot, Brenda Burton, David E Clark
In this paper, we present the results of a ligand- and structure-based virtual screen targeting LRRK2, a kinase that has been implicated in Parkinson's disease. For the ligand-based virtual screen, the structures of 12 competitor compounds were used as queries for a variety of 2D and 3D searches. The structure-based virtual screen relied on homology models of LRRK2, as no X-ray structure is currently available in the public domain. From the virtual screening, 662 compounds were purchased, of which 35 showed IC50 values below 10μM in wild-type and/or mutant LRRK2 (a hit rate of 5...
April 2, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28404093/somatic-pole-proofreading-domain-mutation-immune-response-and-prognosis-in-colorectal-cancer-a-retrospective-pooled-biomarker-study
#8
Enric Domingo, Luke Freeman-Mills, Emily Rayner, Mark Glaire, Sarah Briggs, Louis Vermeulen, Evelyn Fessler, Jan Paul Medema, Arnoud Boot, Hans Morreau, Tom van Wezel, Gerrit-Jan Liefers, Ragnhild A Lothe, Stine A Danielsen, Anita Sveen, Arild Nesbakken, Inti Zlobec, Alessandro Lugli, Viktor H Koelzer, Martin D Berger, Sergi Castellví-Bel, Jenifer Muñoz, Marco de Bruyn, Hans W Nijman, Marco Novelli, Kay Lawson, Dahmane Oukrif, Eleni Frangou, Peter Dutton, Sabine Tejpar, Mauro Delorenzi, Rachel Kerr, David Kerr, Ian Tomlinson, David N Church
BACKGROUND: Precision cancer medicine depends on defining distinct tumour subgroups using biomarkers that may occur at very modest frequencies. One such subgroup comprises patients with exceptionally mutated (ultramutated) cancers caused by mutations that impair DNA polymerase epsilon (POLE) proofreading. METHODS: We examined the association of POLE proofreading domain mutation with clinicopathological variables and immune response in colorectal cancers from clinical trials (VICTOR, QUASAR2, and PETACC-3) and colorectal cancer cohorts (Leiden University Medical Centre 1 and 2, Oslo 1 and 2, Bern, AMC-AJCC-II, and Epicolon-1)...
November 2016: Lancet. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28400847/gengnianchun-a-traditional-chinese-medicine-enhances-oxidative-stress-resistance-and-lifespan-in-caenorhabditis-elegans-by-modulating-daf-16-foxo
#9
Fanhui Meng, Jun Li, Wenjun Wang, Yan Fu
Objective. Gengnianchun (GNC), a traditional Chinese medicine (TCM), is primarily used to improve declining functions related to aging. In this study, we investigated its prolongevity and stress resistance properties and explored the associated regulatory mechanism using a Caenorhabditis elegans model. Methods. Wild-type C. elegans N2 was used for lifespan analysis and oxidative stress resistance assays. Transgenic animals were used to investigate pathways associated with antioxidative stress activity. The effects of GNC on levels of reactive oxygen species (ROS) and expression of specific genes were examined...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28389979/species-adulteration-in-the-herbal-trade-causes-consequences-and-mitigation
#10
REVIEW
Ramanujam Srirama, J U Santhosh Kumar, G S Seethapathy, Steven G Newmaster, S Ragupathy, K N Ganeshaiah, R Uma Shaanker, Gudasalamani Ravikanth
The global economy of the international trade of herbal products has been increasing by 15% annually, with the raw material for most herbal products being sourced from South and Southeast Asian countries. In India, of the 8000 species of medicinal plants harvested from the wild, approximately 960 are in the active trade. With increasing international trade in herbal medicinal products, there is also increasing concern about the widespread adulteration and species admixtures in the raw herbal trade. The adverse consequences of such species adulteration on the health and safety of consumers have only recently begun to be recognised and documented...
April 8, 2017: Drug Safety: An International Journal of Medical Toxicology and Drug Experience
https://www.readbyqxmd.com/read/28385543/rosuvastatin-pharmacokinetics-in-asian-and-white-subjects-wild-type-for-both-oatp1b1-and-bcrp-under-control-and-inhibited-conditions
#11
Hsin-Fang Wu, Nadya Hristeva, Jae Chang, Xiaorong Liang, Ruina Li, Lynda Frassetto, Leslie Z Benet
The FDA recommends rosuvastatin dosage reductions in Asian patients because pharmacokinetic studies have demonstrated an approximate two-fold increase in median exposure to rosuvastatin in Asian subjects when compared to Caucasian controls. Yet, no explanation for this ethnic difference has been confirmed. Here we show that rosuvastatin exposure in Asians and Whites does not differ significantly when all subjects are wildtype carriers for both Solute Carrier Organic anion transporter1B1 *1a and ATP Binding Cassette Subfamily G Member 2 c...
April 3, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28369513/identification-and-characterization-of-aurora-kinase-b-and-c-variants-associated-with-maternal-aneuploidy
#12
Alexandra L Nguyen, Diego Marin, Anbo Zhou, Amanda S Gentilello, Evan M Smoak, Zubing Cao, Anastasia Fedick, Yujue Wang, Deanne Taylor, Richard T Scott, Jinchuan Xing, Nathan Treff, Karen Schindler
STUDY QUESTION: Are single nucleotide variants (SNVs) in Aurora kinases B and C associated with risk of aneuploid conception? SUMMARY ANSWER: Two SNVs were found in patients with extreme aneuploid concepti rates with respect to their age; one variant, AURKC p.I79V, is benign, while another, AURKB p.L39P, is a potential gain-of-function mutant with increased efficiency in promoting chromosome alignment. WHAT IS KNOWN ALREADY: Maternal age does not always predict aneuploidy risk, and rare gene variants can be drivers of disease...
March 25, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28365779/personalized-genome-sequencing-coupled-with-ipsc-technology-identifies-gtdc1-as-a-gene-involved-in-neurodevelopmental-disorders
#13
Irene Aksoy, Kagistia H Utami, Cecilia L Winata, Axel M Hillmer, Sigrid L Rouam, Sylvain Briault, Sonia Davila, Lawrence W Stanton, Valere Cacheux
The cellular and molecular mechanisms underlying neurodevelopmental conditions such as autism spectrum disorders have been studied intensively for decades. The ability to generate patient-specific induced pluripotent stem cells (iPSCs) now offers a novel strategy for modelling human diseases. Recent studies have reported the derivation of iPSCs from patients with neurological disorders. The key challenge remains the demonstration of disease-related phenotypes and the ability to model the disease. Here we report a case study with signs of neurodevelopmental disorders (NDDs) harbouring chromosomal rearrangements that were sequenced using long-insert DNA paired-end tag (DNA-PET) sequencing approach...
January 15, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28362634/site-selective-conjugation-of-an-anticoagulant-aptamer-to-recombinant-albumins-and-maintenance-of-neonatal-fc-receptor-binding
#14
Julie Schmøkel, Anders Voldum, Georgia Tsakiridou, Matthias Kuhlmann, Jason Cameron, Esben S Sørensen, Jesper Wengel, Kenneth A Howard
Aptamers are an attractive molecular medicine that offers high target specificity. Nucleic acid-based aptamers, however, are prone to nuclease degradation and rapid renal excretion that require blood circulatory half-life extension enabling technologies. The long circulatory half-life, predominately facilitated by engagement with the cellular recycling neonatal Fc receptor (FcRn), and ligand transport properties of albumin promote it as an attractive candidate to improve the pharmacokinetic profile of aptamers...
May 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28359898/chitosan-mediated-enhancement-of-hydrolysable-tannin-in-phyllanthus-debilis-klein-ex-willd-via-plant-cell-suspension-culture
#15
Malayaman V, Sisubalan N, Senthilkumar R P, Sheik Mohamed S, Ranjithkumar R, Ghouse Basha M
Phyllanthus debilis Klein ex Willd. is wild medicinal plant used in the traditional system of medicine. This plant has been actively used for hepatoprotection and to cure many diseases including jaundice and so on; which leads to complete extinction of this particular species. Therefore, the chitosan mediated cost effective cell suspension method has been developed for the production of hydrolysable tannin. The hydrolysable tannins are the main therapeutically active constituents with antioxidant, anticancer, and antimicrobial properties...
March 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28359250/insight-into-discovery-of-next-generation-reversible-tmlr-inhibitors-targeting-egfr-activating-and-drug-resistant-t790m-mutants
#16
Subhash Mohan Agarwal, Divyani Pal, Mansi Gupta, Ravi Saini
Cancer is one of the most challenging diseases among the various causes of deaths worldwide. Among cancer, lung cancer rules as the leading cause of cancer-related deaths annually.The majority of the lung cancers identified are non-small cell lung cancer (NSCLC). Clinically, the Epidermal Growth Factor Receptor (EGFR) is a therapeutically accepted target for NSCLC. Many therapeutic leads that target this transmembrane receptor protein were tested for anti-EGFR activity that led to the discovery of gefitinib and erlotinib...
March 30, 2017: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/28350213/true-morels-morchella-nutritional-and-phytochemical-composition-health-benefits-and-flavor-a-review
#17
Zipora Tietel, Segula Masaphy
Morels are edible mushrooms appreciated worldwide for their savory flavor. Morels have been in use in traditional medicine for centuries, due to their health-related benefits, and current research demonstrated their anti-oxidative and anti-inflammatory bioactivities, in addition to immunostimulatory and anti-tumor properties. In spite of the high demand for morels and their increasing economic importance, their cultivation is limited, and they are either used as wild harvested or fermented in culture, for consumption as a functional food and for food-flavoring...
March 28, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28347179/a-sphingosine-type-cerebroside-in-clavicorona-pyxidata-induce-fruit-body-formation
#18
Yongbiao Zheng, Li Xu, Liufeng Wei, Mianmian Shi, Jiyan Zhang, Jianzhong Huang
Clavicorona pyxidata is a wild edible and medicinal mushroom that is rich in bioactive natural products and has thus been extensively used as traditional medicine in China. The present study has determined that the organic crude extract prepared from a fermented culture of C. pyxidata imparted auto-inhibitory effects on mycelial growth and then induced the formation of fruiting bodies. By monitoring bioactivity, one compound was isolated via successive chromatography over silica gel, Sephadex LH-20, and Cl8-reversed phase silica gel and was identified as a known sphingosine-type cerebroside by nuclear magnetic resonance (NMR) and physicochemical data, namely, (4E, 8E)-N-D-2'-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine...
March 28, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28345357/new-bisesquiterpenoid-lactone-from-the-wild-rhizome-of-atractylodes-macrocephala-koidz-grown-in-qimen
#19
Yunzhi Li, Min Dai, Daiyin Peng
The rhizomes of Atractylodes macrocephala are used as both a food source and traditional Chinese medicine in China. A phytochemical investigation was carried out on wild A. macrocephala grown in Qimen County in eastern China, and yielded a novel bisesquiterpenoid lactone, namely, biatractylenolide II (1), along with two known compounds, atractylenolide II (2) and taraxeryl acetate (3). The structure and relative configuration of the new compound were elucidated mainly by 1D and 2D NMR spectroscopic methods in combination with HRESIMS experiments...
March 27, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28343264/modulating-the-ph-activity-profiles-of-phenylalanine-ammonia-lyase-from-anabaena-variabilis-by-modification-of-center-near-surface-residues
#20
Fan Zhang, Nan Huang, Li Zhou, Wenjing Cui, Zhongmei Liu, Longbao Zhu, Yi Liu, Zhemin Zhou
Phenylalanine ammonia lyase from Anabaena variabilis (Av-PAL) is a candidate for the treatment of phenylketonuria (PKU). However, Av-PAL shows its optimal pH at 8.5 and maintains only 70% of its highest activity when pH decreases to 7.3-7.4 (the condition of human plasma). The objective of the study was to shift its optimal pH by mutating surface amino acid residues which interact with the general base Tyr78. Based on the crystal structure and the online program GETAREA, we selected five sites: Asn69, Glu72, Glu75, Asn89, and Val90...
March 25, 2017: Applied Biochemistry and Biotechnology
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