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https://www.readbyqxmd.com/read/29241211/the-role-of-pannexin3-modified-human-dental-pulp-derived-mesenchymal-stromal-cells-in-repairing-rat-cranial-critical-sized-bone-defects
#1
Fangfang Song, Hualing Sun, Liyuan Huang, Dongjie Fu, Cui Huang
BACKGROUND/AIMS: Human dental pulp-derived mesenchymal stromal cells (hDPSCs) are promising seed cells for tissue engineering due to their easy accessibility and multi-lineage differentiation. Pannexin3 (Panx3) plays crucial roles during bone development and differentiation. The aim of the present study was to investigate the effect of Panx3 on osteogenesis of hDPSCs and the underlying mechanism. METHODS: Utilizing qRT-PCR, Western blot, and immunohistochemistry, we explored the change of Panx3 during osteogenic differentiation of hDPSCs...
December 13, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241205/glaucocalyxin-b-alleviates-lipopolysaccharide-induced-parkinson-s-disease-by-inhibiting-tlr-nf-%C3%AE%C2%BAb-and-activating-nrf2-ho-1-pathway
#2
Wei Xu, Deyu Zheng, Yuanyuan Liu, Ji Li, Li Yang, Xiuli Shang
BACKGROUND/AIMS: Parkinson's disease (PD) is a common neurodegenerative disease in the old population, characterized by dopaminergic neuron loss, inflammation and oxidative stress injury in the substantia nigra. Glaucocalyxin B (GLB), an ent-kauranoid diterpenoid isolated from Rabdosia japonica, has anti-inflammation and anti-tumor effects. However, its effects on PD remain unclear. METHODS: PD was introduced in rats via injection of lipopolysaccharide (LPS) into cerebral corpus striatum, and GLB was given intracerebroventricularly to these rats...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241172/neurophysiological-monitoring-and-awake-craniotomy-for-resection-of-intracranial-gliomas
#3
Taiichi Saito, Manabu Tamura, Mikhail F Chernov, Soko Ikuta, Yoshihiro Muragaki, Takashi Maruyama
Aggressive resection of intracranial gliomas has a positive impact on patients' prognosis, but is associated with a risk of neurological complications. For preservation of brain functions and avoidance of major postoperative morbidity various methods of intraoperative neurophysiological monitoring have been introduced into clinical practice. At present, somatosensory evoked potentials (SSEP), motor evoked potentials (MEP), visual evoked potentials (VEP), brainstem auditory evoked potentials (BAEP), and electrocorticography (ECoG) are used routinely during neurosurgical procedures...
2018: Progress in Neurological Surgery
https://www.readbyqxmd.com/read/29241165/sc1-promotes-mir124-3p-expression-to-maintain-the-self-renewal-of-mouse-embryonic-stem-cells-by-inhibiting-the-mek-erk-pathway
#4
Qing Wei, Hongliang Liu, Zhiying Ai, Yongyan Wu, Yingxiang Liu, Zhaopeng Shi, Xuexue Ren, Zekun Guo
BACKGROUND/AIMS: Self-renewal is one of the most important features of embryonic stem (ES) cells. SC1 is a small molecule modulator that effectively maintains the self-renewal of mouse ES cells in the absence of leukemia inhibitory factor (LIF), serum and feeder cells. However, the mechanism by which SC1 maintains the undifferentiated state of mouse ES cells remains unclear. METHODS: In this study, microarray and small RNA deep-sequencing experiments were performed on mouse ES cells treated with or without SC1 to identify the key genes and microRNAs that contributed to self-renewal...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241162/in-vitro-and-in-vivo-activity-of-fomitopsis-pinicola-sw-ex-fr-karst-chloroform-fpkc-extract-against-s180-tumor-cells
#5
Ye Gao, Pan Wang, Yaqin Wang, Lijie Wu, Xiaobing Wang, Kun Zhang, Quanhong Liu
BACKGROUND/AIMS: Non-toxic fomitopsis is has been traditionally used in folk medicine in many countries for its anti-inflammatory and anti-vascular disease activities. The present study investigates the antitumor effect of Fomitopsis pinicola (Sw. Ex Fr.) Karst chloroform extract (FPKc) on S180 tumor cells in vitro and in vivo and we determined the underlying mechanisms. METHODS: HPLC was employed to analyze the constituents of FPKc. In-vitro 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to quantify the growth inhibition of FPKc; Propidium iodide (PI) exclusion assay and scanning electron microscopy (SEM) were used to observe the damage on the cell membrane and the changes of the cell morphology; Staining with Hoechst 33342/propidium iodide (HO/PI) and the application of the Annexin V-FITC/PI analysis permitted to observe the cell death triggered by FPKc; DNA damage and cell cycle arrest were detected by flow cytometry; Rhodamine 123 (RH123) and Cytochrome C were used as dyes to investigate the alterations of the mitochondria...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241161/troxerutin-protects-against-myocardial-ischemia-reperfusion-injury-via-pi3k-akt-pathway-in-rats
#6
Liliang Shu, Wanzhe Zhang, Chen Huang, Gongcheng Huang, Gang Su
BACKGROUND/AIMS: Troxerutin, also known as vitamin P4, has been commonly used in the treatment of chronic venous insufficiency (CVI) disease. However, its effect on in vivo myocardial ischemia/reperfusion (I/R) injury, a model that closely mimics acute myocardial infarction in humans, is still unknown. METHODS: The myocardial I/R injury rat model was created with troxerutin preconditioning. Myocardial infarct size was evaluated by the Evans blue-TTC method. Hemodynamic parameters, including the heart rate (HR), left ventricular end-diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), maximal rate of rise in blood pressure in the ventricular chamber (+dp/dt max), and maximal rate of decline in blood pressure in the ventricular chamber (-dp/dt max) were monitored...
December 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241073/resveratrol-inhibits-proliferation-migration-and-invasion-via-akt-and-erk1-2-signaling-pathways-in-renal-cell-carcinoma-cells
#7
Yuwan Zhao, Huancheng Tang, Xin Zeng, Dongcai Ye, Jianjun Liu
Recent studies have shown that resveratrol (RES) inhibits cancer cell growth, migration and invasion. Here, we evaluated RES in two human renal cell carcinoma (RCC) cell lines, ACHN and A498. We investigated the effects of RES on proliferation, cell morphology, colony formation, migration, and invasion. We used a proliferation assay to demonstrate that RES inhibited cell growth with IC50 values 132.9±1.064μM in ACHN, and 112.8±1.191μM in A498, respectively. Using inverted contrast microscopy, we showed that RES reduced cell-to-cell contact and inhibited formation of filopodia...
December 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29241070/protocatechuic-acid-ameliorates-high-glucose-induced-extracellular-matrix-accumulation-in-diabetic-nephropathy
#8
Yali Ma, Fang Chen, Suxia Yang, Baoping Chen, Jun Shi
Protocatechuic acid (PCA), a phenolic compound of anthocyanins, was reported to possess various pharmacologic properties, including anti-oxidant, anti-inflammatory, anti-apoptosis, anti-diabetic and anti-tumor activities. However, the role of PCA in diabetic nephropathy remains elusive. The present study was conducted to evaluate the effects of PCA on extracellular matrix (ECM) accumulation in high glucose (HG)-induced human mesangial cells (MCs) and explore the possible mechanism. Our results demonstrated that PCA obviously inhibited HG-induced proliferation of MCs in a dose-dependent manner...
December 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29241031/the-protective-effect-of-dexmedetomidine-on-lps-induced-acute-lung-injury-through-the-hmgb1-mediated-tlr4-nf-%C3%AE%C2%BAb-and-pi3k-akt-mtor-pathways
#9
Lu Meng, Longyun Li, Shan Lu, Kai Li, Zhenbo Su, Yunyun Wang, Xiaodi Fan, Xuyang Li, Guoqing Zhao
The aim of present study was to evaluate the protective effects of dexmedetomidine (DEX) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and investigate its possible mechanisms mediated by HMGB1. In vivo, pulmonary pathology observation and myeloperoxidase (MPO) activity were also examined to evaluate the protective effect of DEX in the lungs. Tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in bronchoalveolar lavage fluid (BALF), serum and lung tissues LPS-induced rats were detected...
December 11, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29240949/nitric-oxide-production-in-plants-an-update
#10
Jeremy Astier, Inonge Gross, Jörg Durner
Nitric oxide (NO) is a key signaling molecule in plant physiology. However, its production in photosynthetic organisms remains partially unresolved. The best characterized NO production route involves the reduction of nitrite to NO via different non-enzymatic or enzymatic mechanisms. Nitrate reductases (NRs), the mitochondrial electron transport chain, and the new complex between NR and NOFNiR (nitric oxide-forming nitrite reductase) described in Chlamydomonas reinhardtii are the main enzymatic systems that perform this reductive NO production in plants...
December 12, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29240948/early-cyclical-neuromuscular-electrical-stimulation-improves-strength-and-trophism-by-akt-pathway-signaling-in-partially-paralyzed-biceps-muscle-after-spinal-cord-injury-in-rats
#11
Gabriel R de Freitas, Caroline C do Espírito Santo, Nicolas A M M de Machado-Pereira, Franciane Bobinski, Adair R S Dos Santos, Jocemar Ilha
Background: Electrical stimulation is often used to treat weakness in people with spinal cord injury (SCI), however its efficacy to increase strength and trophism are weak and the mechanisms underlying the therapeutic benefits are unknown. Objective: The purpose of the study was to analyze the effects of neuromuscular electrical stimulation (NMES) on muscle function, trophism and the Akt pathway signaling involved in muscular plasticity after incomplete-SCI in rats...
December 12, 2017: Physical Therapy
https://www.readbyqxmd.com/read/29240839/the-soluble-loop-bc-region-guides-but-not-dictates-the-assembly-of-the-transmembrane-cytochrome-b6
#12
Lydia Tome-Stangl, Cornelia Schaetzel, Stefan Tenzer, Frank Bernhard, Dirk Schneider
Studying folding and assembly of naturally occurring α-helical transmembrane proteins can inspire the design of membrane proteins with defined functions. Thus far, most studies have focused on the role of membrane-integrated protein regions. However, to fully understand folding pathways and stabilization of α-helical membrane proteins, it is vital to also include the role of soluble loops. We have analyzed the impact of interhelical loops on folding, assembly and stability of the heme-containing four-helix bundle transmembrane protein cytochrome b6 that is involved in charge transfer across biomembranes...
2017: PloS One
https://www.readbyqxmd.com/read/29240812/effects-of-vaspin-on-pancreatic-%C3%AE-cell-secretion-via-pi3k-akt-and-nf-%C3%AE%C2%BAb-signaling-pathways
#13
Shiwei Liu, Xin Li, Yaru Wu, Ruixue Duan, Jiaxin Zhang, Fang Du, Qi Zhang, Yuanbin Li, Naishi Li
Vaspin (visceral adipose tissue-derived serine protease inhibitor) is a recently discovered adipokine that has been implicated in diabetes mellitus and other metabolic disorders. However, the effects of vaspin on pancreatic β cell function and related mechanisms are not fully understood. Thus, the present study was performed to investigate the effects of vaspin on pancreatic β cell function and the potential underlying mechanisms. Both in vitro (rat insulinoma cells, INS-1) and in vivo (high fat diet fed rats) experiments were conducted...
2017: PloS One
https://www.readbyqxmd.com/read/29240787/brd3-4-inhibition-and-flt3-ligand-deprivation-target-pathways-that-are-essential-for-the-survival-of-human-mll-af9-leukemic-cells
#14
Marco Carretta, Annet Z Brouwers-Vos, Matthieu Bosman, Sarah J Horton, Joost H A Martens, Edo Vellenga, Jan Jacob Schuringa
In the present work we aimed to identify targetable signaling networks in human MLL-AF9 leukemias. We show that MLL-AF9 cells critically depend on FLT3-ligand induced pathways as well as on BRD3/4 for their survival. We evaluated the in vitro and in vivo efficacy of the BRD3/4 inhibitor I-BET151 in various human MLL-AF9 (primary) models and patient samples and analyzed the transcriptome changes following treatment. To further understand the mode of action of BRD3/4 inhibition, we performed ChIP-seq experiments on the MLL-AF9 complex in THP1 cells and compared it to RNA-seq data of I-BET151 treated cells...
2017: PloS One
https://www.readbyqxmd.com/read/29240764/selection-for-long-and-short-sleep-duration-in-drosophila-melanogaster-reveals-the-complex-genetic-network-underlying-natural-variation-in-sleep
#15
Susan T Harbison, Yazmin L Serrano Negron, Nancy F Hansen, Amanda S Lobell
Why do some individuals need more sleep than others? Forward mutagenesis screens in flies using engineered mutations have established a clear genetic component to sleep duration, revealing mutants that convey very long or short sleep. Whether such extreme long or short sleep could exist in natural populations was unknown. We applied artificial selection for high and low night sleep duration to an outbred population of Drosophila melanogaster for 13 generations. At the end of the selection procedure, night sleep duration diverged by 9...
December 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29240760/novel-linear-motif-filtering-protocol-reveals-the-role-of-the-lc8-dynein-light-chain-in-the-hippo-pathway
#16
Gábor Erdős, Tamás Szaniszló, Mátyás Pajkos, Borbála Hajdu-Soltész, Bence Kiss, Gábor Pál, László Nyitray, Zsuzsanna Dosztányi
Protein-protein interactions (PPIs) formed between short linear motifs and globular domains play important roles in many regulatory and signaling processes but are highly underrepresented in current protein-protein interaction databases. These types of interactions are usually characterized by a specific binding motif that captures the key amino acids shared among the interaction partners. However, the computational proteome-level identification of interaction partners based on the known motif is hindered by the huge number of randomly occurring matches from which biologically relevant motif hits need to be extracted...
December 14, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/29240755/integrating-transcriptomics-and-metabolomics-to-characterize-the-regulation-of-epa-biosynthesis-in-response-to-cold-stress-in-seaweed-bangia-fuscopurpurea
#17
Min Cao, Dongmei Wang, Yunxiang Mao, Fanna Kong, Guiqi Bi, Qikun Xing, Zhen Weng
Bangia fuscopurpurea is a traditional mariculture crop having high nutritional value, eicosapntemacnioc acid (EPA) production, and protein content. As an intertidal species, it can tolerate drastic changes in abiotic factors such as temperature, hydration, and light radiation; however, genomic information on the evolutionary aspect and mechanism of EPA enrichment in B. fuscopurpurea and the role of EPA in cold tolerance in this species remain elusive. We conducted transcriptome profile analysis in B. fuscopurpurea to investigate the biological functions of genes associated with resistance to various environment factors...
2017: PloS One
https://www.readbyqxmd.com/read/29240740/characteristics-of-and-differences-between-pasifika-women-and-new-zealand-european-women-diagnosed-with-breast-cancer-in-new-zealand
#18
Charis Brown, Chunhuan Lao, Ross Lawrenson, Sandar Tin Tin, Michelle Schaaf, Jacquie Kidd, Anne Allan-Moetaua, Josephine Herman, Reena Raamsroop, Ian Campbell, Mark Elwood
AIM: Breast cancer in New Zealand-based Pasifika women is a significant issue. Although Pasifika women have a lower incidence of breast cancer compared to New Zealand European women, they have higher breast cancer mortality and lower five-year survival. The aim of this study was to describe the characteristics and tumour biology of Pasifika women and to compare New Zealand European women to identify what factors impact on early (Stage 1 and 2) vs advanced stage (Stage 3 and 4) at diagnosis...
December 15, 2017: New Zealand Medical Journal
https://www.readbyqxmd.com/read/29240671/microwave-enhanced-photolysis-of-norfloxacin-kinetics-matrix-effects-and-degradation-pathways
#19
Wenchao Liao, Virender K Sharma, Su Xu, Qingsong Li, Lei Wang
Degradation of norfloxacin (NOR) was studied using a combination of microwave and UV irradiation methods (MW/UV process). Remarkable synergistic effect was found between MW and UV light. The removal rate with the MW/UV process was much faster than that with UV light irradiation only. Degradation of NOR followed second-order kinetics and ~72% of NOR could be removed in the first 5 min of MW/UV reaction. Influence of inorganic ions (cations (K⁺, Mg2+, Ca2+, Cu2+) and anions (Cl-, SO₄2-, NO₃-, CO₃2-)), humic acid (HA) and surfactants (cation, anion, and non-ionic) on the degradation of NOR by the MW/UV process was investigated...
December 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29240665/airborne-or-fomite-transmission-for-norovirus-a-case-study-revisited
#20
Shenglan Xiao, Julian W Tang, Yuguo Li
Norovirus infection, a highly prevalent condition associated with a high rate of morbidity, comprises a significant health issue. Although norovirus transmission mainly occurs via the fecal-oral and vomit-oral routes, airborne transmission has been proposed in recent decades. This paper re-examines a previously described norovirus outbreak in a hotel restaurant wherein airborne transmission was originally inferred. Specifically, the original evidence that suggested airborne transmission was re-analyzed by exploring an alternative hypothesis: could this outbreak instead have occurred via fomite transmission? This re-analysis was based on whether fomite transmission could have yielded similar attack rate distribution patterns...
December 14, 2017: International Journal of Environmental Research and Public Health
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