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Spindle Assembly Checkpoint

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https://www.readbyqxmd.com/read/27885363/mitotic-protein-kinase-1-role-in-spindle-assembly-checkpoint-revisited
#1
Anita Tandle, Kevin Camphausen
No abstract text is available yet for this article.
May 2016: J Cancer Clin Trials
https://www.readbyqxmd.com/read/27881301/structure-of-the-mis12-complex-and-molecular-basis-of-its-interaction-with-cenp-c-at-human-kinetochores
#2
Arsen Petrovic, Jenny Keller, Yahui Liu, Katharina Overlack, Juliane John, Yoana N Dimitrova, Simon Jenni, Suzan van Gerwen, Patricia Stege, Sabine Wohlgemuth, Pascaline Rombaut, Franz Herzog, Stephen C Harrison, Ingrid R Vetter, Andrea Musacchio
Kinetochores, multisubunit protein assemblies, connect chromosomes to spindle microtubules to promote chromosome segregation. The 10-subunit KMN assembly (comprising KNL1, MIS12, and NDC80 complexes, designated KNL1C, MIS12C, and NDC80C) binds microtubules and regulates mitotic checkpoint function through NDC80C and KNL1C, respectively. MIS12C, on the other hand, connects the KMN to the chromosome-proximal domain of the kinetochore through a direct interaction with CENP-C. The structural basis for this crucial bridging function of MIS12C is unknown...
November 3, 2016: Cell
https://www.readbyqxmd.com/read/27874987/retraction
#3
(no author information available yet)
This article corrects: Retracted: Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis. New Phytologist 205: 202-215. Article first published online: 29 September 2014. This article has been retracted at the request of: Editor-in-Chief and Author 'Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis', by Paganelli L, Caillaud M-C, Quentin M, Damiani I, Govetto B, Lecomte P, Karpov, PA, Abad P, Chabouté M-E and Favery B...
December 2016: New Phytologist
https://www.readbyqxmd.com/read/27869759/a-cell-biological-perspective-on-past-present-and-future-investigations-of-the-spindle-assembly-checkpoint
#4
REVIEW
Ajit P Joglekar
The spindle assembly checkpoint (SAC) is a quality control mechanism that ensures accurate chromosome segregation during cell division. It consists of a mechanochemical signal transduction mechanism that senses the attachment of chromosomes to the spindle, and a signaling cascade that inhibits cell division if one or more chromosomes are not attached. Extensive investigations of both these component systems of the SAC have synthesized a comprehensive understanding of the underlying molecular mechanisms. This review recounts the milestone results that elucidated the SAC, compiles a simple model of the complex molecular machinery underlying the SAC, and highlights poorly understood facets of the biochemical design and cell biological operation of the SAC that will drive research forward in the near future...
November 19, 2016: Biology
https://www.readbyqxmd.com/read/27848932/lack-of-diaph3-relaxes-the-spindle-checkpoint-causing-the-loss-of-neural-progenitors
#5
Devid Damiani, André M Goffinet, Arthur Alberts, Fadel Tissir
The diaphanous homologue Diaph3 (aka mDia2) is a major regulator of actin cytoskeleton. Loss of Diaph3 has been constantly associated with cytokinesis failure ascribed to impaired accumulation of actin in the cleavage furrow. Here we report that Diaph3 is required before cell fission, to ensure the accurate segregation of chromosomes. Inactivation of the Diaph3 gene causes a massive loss of cortical progenitor cells, with subsequent depletion of intermediate progenitors and neurons, and results in microcephaly...
November 16, 2016: Nature Communications
https://www.readbyqxmd.com/read/27819146/mps1-ttk-a-novel-target-and-biomarker-for-cancer
#6
Yuan Xie, Anqiang Wang, Jianzhen Lin, Liangcai Wu, Haohai Zhang, Xiaobo Yang, Xueshuai Wan, Ruoyu Miao, Xinting Sang, Haitao Zhao
Monopolar spindle1 (Mps1, also known as TTK) is the core component of the spindle assembly checkpoint, which functions to ensure proper distribution of chromosomes to daughter cells. Mps1 is hardly detectable in normal organs except the testis and placenta. However, high levels of Mps1 are found in many types of human malignancies, including glioblastoma, thyroid carcinoma, breast cancer, and other cancers. Several Mps1 inhibitors can inhibit the proliferation of cancer cells and exhibit demonstrable survival benefits...
December 1, 2016: Journal of Drug Targeting
https://www.readbyqxmd.com/read/27818790/bub1-and-survivin-proteins-are-not-degraded-after-a-prolonged-mitosis-and-accumulate-in-the-nuclei-of-hct116-cells
#7
Marco A Andonegui-Elguera, Rodrigo E Cáceres-Gutiérrez, Fernando Luna-Maldonado, Alejandro López-Saavedra, José Díaz-Chávez, Fernanda Cisneros-Soberanis, Diddier Prada, Julia Mendoza-Pérez, Luis A Herrera
Spindle poisons activate the spindle assembly checkpoint and prevent mitotic exit until cells die or override the arrest. Several studies have focused on spindle poison-mediated cell death, but less is known about consequences in cells that survive a mitotic arrest. During mitosis, proteins such as CYCLIN B, SECURIN, BUB1 and SURVIVIN are degraded in order to allow mitotic exit, and these proteins are maintained at low levels in the next interphase. In contrast, exit from a prolonged mitosis depends only on degradation of CYCLIN B; it is not known whether the levels of other proteins decrease or remain high...
2016: Cell Death Discovery
https://www.readbyqxmd.com/read/27816818/the-spindle-checkpoint-in-plants-a-green-variation-over-a-conserved-theme
#8
REVIEW
Shinichiro Komaki, Arp Schnittger
The spindle checkpoint, also called spindle assembly checkpoint (SAC), is a crucial control instance in animals and yeast that surveys the correct attachment of chromosomes to the spindle assuring their equal distribution in mitosis and meiosis. The presence of homologs of all core SAC components in plants indicates that these regulators have an ancient function. However, the fact that mutants of SAC components in plants are usually fully viable together with the observation that plants can be readily made polyploid raises the question whether plants have an efficient SAC...
October 27, 2016: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/27815897/synchronization-and-desynchronization-of-cells-by-interventions-on-the-spindle-assembly-checkpoint
#9
Mohamed Jemaà, Gwenola Manic, Ilio Vitale
Cell cycle checkpoints are surveillance mechanisms that sequentially and continuously monitor cell cycle progression thereby contributing to the preservation of genetic stability. Among them, the spindle assembly checkpoint (SAC) prevents the occurrence of abnormal divisions by halting the metaphase to anaphase transition following the detection of erroneous microtubules-kinetochore attachment(s). Most synchronization strategies are based on the activation of cell cycle checkpoints to enrich the population of cells in a specific phase of the cell cycle...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27815460/bubr1-insufficiency-in-mice-increases-their-sensitivity-to-oxidative-stress
#10
Daisuke Matsuda, Takuya Matsumoto, Kenichi Honma, Ayae Ikawa-Yoshida, Mitsuho Onimaru, Tadashi Furuyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yoshihiko Maehara
BACKGROUND/AIM: Budding uninhibited by benzimidazole-related 1 (BUBR1) plays an important role in the spindle assembly checkpoint to prevent chromosome missegregation and aneuploidy during mitosis. We previously generated mutant mice that express BUBR1 at only 20% of the normal level (BubR1(L/L) mice). Here, we examined the effect of low BUBR1 expression on oxidative stress-induced carcinogenesis in mice. MATERIALS AND METHODS: We orally administered either a potassium bromate (KBrO3) solution (2 g/l) or tap water to BubR1(L/L) and wild-type (BubR1(+/+))mice for 16 weeks and examined the subsequent incidence of tumours...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27810909/the-ubiquitin-ligase-crl2zyg11-targets-cyclin-b1-for-degradation-in-a-conserved-pathway-that-facilitates-mitotic-slippage
#11
Riju S Balachandran, Cassandra S Heighington, Natalia G Starostina, James W Anderson, David L Owen, Srividya Vasudevan, Edward T Kipreos
The anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase is known to target the degradation of cyclin B1, which is crucial for mitotic progression in animal cells. In this study, we show that the ubiquitin ligase CRL2(ZYG-11) redundantly targets the degradation of cyclin B1 in Caenorhabditis elegans and human cells. In C. elegans, both CRL2(ZYG-11) and APC/C are required for proper progression through meiotic divisions. In human cells, inactivation of CRL2(ZYG11A/B) has minimal effects on mitotic progression when APC/C is active...
October 24, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27810907/slip-slidin-away-of-mitosis-with-crl2zyg11
#12
Michael Brandeis
The spindle assembly checkpoint arrests mitotic cells by preventing degradation of cyclin B1 by the anaphase-promoting complex/cyclosome, but some cells evade this checkpoint and slip out of mitosis. Balachandran et al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201601083) show that the E3 ligase CRL2(ZYG11) degrades cyclin B1, allowing mitotic slippage.
October 24, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27798241/an-acetyltransferase-independent-function-of-eso1-regulates-centromere-cohesion
#13
Su-Jiun Lin, Claudia Tapia-Alveal, Omar J Jabado, Doris Germain, Matthew J O'Connell
Eukaryotes contain three essential Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6. Cohesin forms a ring-shaped structure that embraces sister chromatids to promote their cohesion. The cohesiveness of cohesin is promoted by acetylation of N-terminal lysines of the Smc3 subunit by the acetyltransferases Eco1 in Saccharomyces cerevisiae, and by the homolog Eso1 in Schizosaccharomyces pombe In both yeasts, these acetyltransferases are essential for cell viability. However, while non-acetylatable Smc3 mutants are lethal in S...
October 19, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27791030/mitotic-golgi-disassembly-is-required-for-bipolar-spindle-formation-and-mitotic-progression
#14
Gianni Guizzunti, Joachim Seemann
During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metaphase. To prevent Golgi disassembly, we expressed HRP linked to a Golgi-resident protein and acutely triggered the polymerization of 3,3'-diaminobenzidine (DAB) in the Golgi lumen. The DAB polymer does not affect interphase cell viability, but inhibits Golgi fragmentation by nocodazole and brefeldin A and also halts cells in early mitosis...
October 25, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27742609/a-novel-mutation-in-the-n-terminal-domain-of-drosophila-bubr1-affects-the-spindle-assembly-checkpoint-function-of-bubr1
#15
Marie Duranteau, Jean-Jacques Montagne, Zohra Rahmani
The spindle assembly checkpoint (SAC) is a surveillance mechanism that ensures accurate segregation of chromosomes into two daughter cells. BubR1, a key component of the SAC, also plays a role in the mitotic timing since depletion of BubR1 leads to accelerated mitosis. We previously found that mutation of the KEN1-box domain of Drosophila BubR1 (bubR1-KEN1 mutant) affects the binding of BubR1 to Cdc20, the activating co-factor of the APC/C, and does not accelerate the mitotic timing despite resulting in a defective SAC, which was unlike what was reported in mammalian cells...
November 15, 2016: Biology Open
https://www.readbyqxmd.com/read/27731420/whole-chromosome-instability-induces-senescence-and-promotes-sasp
#16
Grasiella Angelina Andriani, Vinnycius Pereira Almeida, Francesca Faggioli, Maurizio Mauro, Wanxia Li Tsai, Laura Santambrogio, Alexander Maslov, Massimo Gadina, Judith Campisi, Jan Vijg, Cristina Montagna
Age-related accumulation of ploidy changes is associated with decreased expression of genes controlling chromosome segregation and cohesin functions. To determine the consequences of whole chromosome instability (W-CIN) we down-regulated the spindle assembly checkpoint component BUB1 and the mitotic cohesin SMC1A, and used four-color-interphase-FISH coupled with BrdU incorporation and analyses of senescence features to reveal the fate of W-CIN cells. We observed significant correlations between levels of not-diploid cells and senescence-associated features (SAFs)...
October 12, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27713168/the-aurora-kinase-a-inhibitor-tc-a2317-disrupts-mitotic-progression-and-inhibits-cancer-cell-proliferation
#17
Yoo Hong Min, Wootae Kim, Ja-Eun Kim
Mitotic progression is crucial for the maintenance of chromosomal stability. A proper progression is ensured by the activities of multiple kinases. One of these enzymes, the serine/threonine kinase Aurora A, is required for proper mitosis through the regulation of centrosome and spindle assembly. In this study, we functionally characterized a newly developed Aurora kinase A inhibitor, TC-A2317. In human lung cancer cells, TC-A2317 slowed proliferation by causing aberrant formation of centrosome and microtubule spindles and prolonging the duration of mitosis...
October 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27713128/spc24-is-required-for-meiotic-kinetochore-microtubule-attachment-and-production-of-euploid-eggs
#18
Teng Zhang, Yang Zhou, Hong-Hui Wang, Tie-Gang Meng, Lei Guo, Xue-Shan Ma, Wei Shen, Heide Schatten, Qing-Yuan Sun
Mammalian oocytes are particularly error prone in chromosome segregation during two successive meiotic divisions. The proper kinetochore-microtubule attachment is a prerequisite for faithful chromosome segregation during meiosis. Here, we report that Spc24 localizes at the kinetochores during mouse oocyte meiosis. Depletion of Spc24 using specific siRNA injection caused defective kinetochore-microtubule attachments and chromosome misalignment, and accelerated the first meiosis by abrogating the kinetochore recruitment of spindle assembly checkpoint protein Mad2, leading to a high incidence of aneuploidy...
October 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27713084/pseudolaric-acid-b-induces-mitotic-arrest-and-apoptosis-in-both-5-fluorouracil-sensitive-and-resistant-colorectal-cancer-cells
#19
Chuangyu Wen, Junxiong Chen, Di Zhang, Huihui Wang, Jia Che, Qiyuan Qin, Lu He, Zerong Cai, Mengmeng Lin, Qiong Lou, Lanlan Huang, Daici Chen, Aikichi Iwamoto, Donglin Ren, Lei Wang, Ping Lan, Jianping Wang, Huanliang Liu, Xiangling Yang
5-fluorouracil (5-FU)-based chemotherapy is the main chemotherapeutic approach for colorectal cancer (CRC) treatment. Because chemoresistance occurs frequently and significantly limits CRC therapies, a novel agent is needed. Pseudolaric acid B (PAB), a small molecule derived from the Chinese medicinal herb ''Tujinpi'', exhibits strong cytotoxic effects on a variety of cancers. However, the detailed mechanisms by which PAB inhibits CRC cell growth and its potential role in overcoming 5-FU resistance have not been well studied...
October 3, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27697865/the-inositol-pyrophosphate-kinase-asp1-modulates-chromosome-segregation-fidelity-and-spindle-function-in-s-pombe
#20
Boris Topolski, Visnja Jakopec, Natascha A Künzel, Ursula Fleig
Chromosome transmission fidelity during mitosis is of critical importance for the fitness of an organism as mistakes will lead to aneuploidy, which has a causative role in numerous severe diseases. Proper segregation of chromosomes depends on interdependent processes at the microtubule-kinetochore interface and the spindle assembly checkpoint. Here we report the discovery of a new element essential for chromosome transmission fidelity that implicates inositol pyrophosphates (IPPs) as playing a key role in this process...
October 3, 2016: Molecular and Cellular Biology
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