Daisuke Ogasawara, David B Konrad, Zher Yin Tan, Kimberly L Carey, Jessica Luo, Sang Joon Won, Haoxin Li, Trever Carter, Kristen E DeMeester, Evert Njomen, Stuart L Schreiber, Ramnik J Xavier, Bruno Melillo, Benjamin F Cravatt
Chemical proteomics enables the global assessment of small molecule-protein interactions in native biological systems and has emerged as a versatile approach for ligand discovery. The range of small molecules explored by chemical proteomics has, however, been limited. Here, we describe a diversity-oriented synthesis (DOS)-inspired library of stereochemically-defined compounds bearing diazirine and alkyne units for UV light-induced covalent modification and click chemistry enrichment of interacting proteins, respectively...
February 29, 2024: bioRxiv