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https://www.readbyqxmd.com/read/28819851/rarecyte-%C3%A2-ctc-analysis-step-3-using-the-cytepicker-%C3%A2-module-for-individual-cell-retrieval-and-subsequent-whole-genome-amplification-of-circulating-tumor-cells-for-genomic-analysis
#1
Jackie L Stilwell, Paulina Varshavskaya, Jeffrey L Werbin, Joshua J Nordberg, Arturo B Ramirez, Steve Quarre, Jay Tzucker, Jennifer Chow, Brennan Enright, Eric P Kaldjian
The CytePicker module built into the RareCyte CyteFinder instrument allows researchers to easily retrieve individual cells from microscope slides for genomic analyses, including array CGH, targeted sequencing, and next-generation sequencing. Here, we describe the semiautomated retrieval of CTCs from the blood processed by AccuCyte (see Chapter 13) and amplification of genomic DNA so that molecular analysis can be performed.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819850/rarecyte-%C3%A2-ctc-analysis-step-2-detection-of-circulating-tumor-cells-by-cytefinder-%C3%A2-automated-scanning-and-semiautomated-image-analysis
#2
Jeffrey L Werbin, Joshua J Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L Stilwell, Eric P Kaldjian
The RareCyte CyteFinder instrument is an automated scanner that allows rapid identification of circulating tumor cells (CTCs) on microscope slides prepared by the AccuCyte process (see Chapter 13 ) and stained by immunofluorescence. Here, we present the workflow for CyteFinder scanning, analysis, and CyteMapper scan review which includes CTC confirmation and report generation.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819849/rarecyte%C3%A2-ctc-analysis-step-1-accucyte%C3%A2-sample-preparation-for-the-comprehensive-recovery-of-nucleated-cells-from-whole-blood
#3
Arturo B Ramirez, Lance U'Ren, Daniel E Campton, David Stewart, Joshua J Nordberg, Jackie L Stilwell, Eric P Kaldjian
The RareCyte platform addresses important technology limitations of current circulating tumor cell (CTC) collection methods, and expands CTC interrogation to include advanced phenotypic characterization and single-cell molecular analysis. In this respect, it represents the "next generation" of cell-based liquid biopsy technologies. In order to identify and analyze CTCs, RareCyte has developed an integrated sample preparation, imaging and individual cell retrieval process. The first step in the process, AccuCyte(®), allows the separation, collection, and transfer to a slide the nucleated cell fraction of the blood that contains CTCs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28817478/more-than-1-million-potential-second-victims-how-many-could-nursing-education-prevent
#4
Jackie H Jones, Linda A Treiber
Any error made in health care can cause the health care provider to become a second victim. There are many initiatives, tools, and instruments designed to support second victims after an error has been made. The role that nursing education can play in preventing nurses from becoming second victims has not been well explored. This article presents a study designed to investigate perceptions of recent BSN graduates about preparation for medication administration, medication error, and their personal experience with error making and second victimhood...
August 16, 2017: Nurse Educator
https://www.readbyqxmd.com/read/28816805/development-of-a-mobile-app-for-family-planning-providers
#5
Viannella Halsall, Jennifer Rogers, Jacki Witt, Sejun Song, Hoang Duc Huy Nguyen, Patricia Kelly
PURPOSE: To provide an overview of lessons learned during the development process of an app for iOS and Android based on national recommendations for providing quality family planning services. STUDY AND DESIGN: After a review of existing apps was conducted to determine whether an app of clinical recommendations for family planning existed, a team of clinicians, training specialists, and app developers created a resource app by first drafting a comprehensive content map...
September 2017: MCN. the American Journal of Maternal Child Nursing
https://www.readbyqxmd.com/read/28815974/health-promotion-in-fiji-is-it-feasible-in-the-emergency-department
#6
Anne Creaton, Jackie Holt
OBJECTIVES: Complications of diabetes, hypertension and asthma are commonly seen in EDs in Fiji. Time spent in the ED offers an opportunity for health promotion activities. The aim of the intervention was to provide information on diabetes, hypertension and asthma to patients in the ED. The aim of the project was to determine whether such an intervention could be implemented using public health students. The aim of this paper is to document the experiences of those who delivered and supervised the intervention...
August 16, 2017: Emergency Medicine Australasia: EMA
https://www.readbyqxmd.com/read/28812726/biocontrol-of-common-carp-in-australia-poses-risks-to-biosecurity
#7
Jackie Lighten, Cock van Oosterhout
No abstract text is available yet for this article.
February 21, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28810688/exhaled-breath-condensate-a-novel-matrix-for-biological-monitoring-to-assess-occupational-exposure-to-respirable-crystalline-silica
#8
Elizabeth Leese, James F Staff, Vikki A Carolan, Jackie Morton
Biological monitoring (BM) is a useful way of determining overall exposures to chemical substances; however, in the case of respirable crystalline silica (RCS), this has not been analytically feasible in conventional biological matrices. The aim of this study was to investigate the utility of exhaled breath condensate (EBC) as a potential biological matrix in which to determine exposure to RCS. A small pilot study was undertaken collecting EBC from six quarry workers and six occupationally unexposed persons; the samples were analysed using both single particle inductively coupled plasma mass spectrometry (spICP-MS) and transmission electron microscopy (TEM)...
August 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28802698/in-hospital-costs-of-diabetic-foot-disease-treated-by-a-multidisciplinary-foot-team
#9
Willem D Rinkel, Jacky Luiten, Jelle van Dongen, Bram Kuppens, Johan W Van Neck, Suzanne Polinder, Manuel Castro Cabezas, J Henk Coert
BACKGROUND: The diabetic foot imposes significant burden on healthcare systems. Obtaining knowledge on the extent of the costs of diabetic foot ulcers (DFUs) is of value to health care researchers investigating cost-effectiveness of interventions that prevent these costly complications. OBJECTIVES: To estimate the in-hospital costs associated with the treatment of DFUs by a multidisciplinary diabetic foot team. METHODS: Persons with DFUs presenting to our team in 2013 and 2014 were followed and use of care was estimated...
July 29, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28802538/what-is-the-reliability-of-clinical-measurement-tests-for-humeral-head-position-a-systematic-review
#10
Christine Konieczka, Christine Gibson, Leeann Russett, Leah Dlot, Joy MacDermid, Lyn Watson, Jackie Sadi
STUDY DESIGN: Systematic review. INTRODUCTION: Physiotherapists routinely assess the position of the humeral head (HH) in patients with shoulder pain. PURPOSE OF THE STUDY: To conduct a systematic review to determine the quality and content of studies that evaluated the reliability of clinical measurement methods for assessing the HH position. METHODS: Five databases and gray literature were searched for studies fitting the eligibility criteria...
August 9, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/28800182/development-and-preliminary-pilot-evaluation-of-a-brief-tablet-computer-intervention-to-motivate-tobacco-quitline-use-among-smokers-in-substance-use-treatment
#11
Richard A Brown, Jacki Hecht, Erika L Bloom, Haruka Minami, Christopher W Kahler, Ana M Abrantes, Mary E Dubreuil, Alan Gordon, Lawrence H Price, Steven J Ondersma
BACKGROUND AND OBJECTIVES: The majority of individuals in substance use disorder (SUD) treatment also smoke cigarettes; yet, the availability of smoking cessation services in SUD treatment remains limited. In this study, we developed and piloted a brief intervention for smokers in SUD treatment intended to motivate engagement in tobacco quitline treatment (TIME-TQ). METHODS: First, we interviewed 19 smokers in SUD treatment to inform the development of TIME-TQ (Phase 1)...
August 11, 2017: American Journal on Addictions
https://www.readbyqxmd.com/read/28799114/neurogenic-stress-cardiomyopathy-precipitated-by-acute-hydrocephalus-after-aneurysmal-subarachnoid-hemorrhage
#12
Kamel Gharaibeh, Jackie Scott, Nicholas A Morris
No abstract text is available yet for this article.
August 10, 2017: Neurocritical Care
https://www.readbyqxmd.com/read/28797529/voice-specialized-speech-language-pathologist-s-criteria-for-discharge-from-voice-therapy
#13
Amanda I Gillespie, Jackie Gartner-Schmidt
OBJECTIVE: No standard protocol exists to determine when a patient is ready and able to be discharged from voice therapy. The aim of the present study was to determine what factors speech-language pathologists (SLPs) deem most important when discharging a patient from voice therapy. A second aim was to determine if responses differed based on years of voice experience. METHODS: Step 1: Seven voice-specialized SLPs generated a list of items thought to be relevant to voice therapy discharge...
August 7, 2017: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/28796370/a-highly-sensitive-bimodal-detection-of-amine-vapours-based-on-aggregation-induced-emission-of-1-2-dihydroquinoxaline-derivatives
#14
Parvej Alam, Nelson Lik Ching Leung, Huifang Su, Zijie Qiu, Ryan T K Kwok, Jacky W Y Lam, Ben Zhong Tang
The detection of food spoilage is a major concern in food safety as large amounts of food are transported globally. Direct analysis of food samples is often time-consuming and requires expensive analytical instrumentation. A much simpler and more cost-effective method for monitoring food fermentation is to detect biogenic amines generated as a by-product during food decomposition. In this work, a series of 1,2-dihydroquinoxaline derivatives (DQs) with aggregation-induced emission (AIE) characteristics were synthesized and their protonated forms, i...
August 10, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28792880/analysis-of-plasma-epstein-barr-virus-dna-to-screen-for-nasopharyngeal-cancer
#15
K C Allen Chan, John K S Woo, Ann King, Benny C Y Zee, W K Jacky Lam, Stephen L Chan, Sam W I Chu, Constance Mak, Irene O L Tse, Samantha Y M Leung, Gloria Chan, Edwin P Hui, Brigette B Y Ma, Rossa W K Chiu, Sing-Fai Leung, Andrew C van Hasselt, Anthony T C Chan, Y M Dennis Lo
BACKGROUND: Circulating cell-free Epstein-Barr virus (EBV) DNA is a biomarker for nasopharyngeal carcinoma. We conducted a prospective study to investigate whether EBV DNA in plasma samples would be useful to screen for early nasopharyngeal carcinoma in asymptomatic persons. METHODS: We analyzed EBV DNA in plasma specimens to screen participants who did not have symptoms of nasopharyngeal carcinoma. Participants with initially positive results were retested approximately 4 weeks later, and those with persistently positive EBV DNA in plasma underwent nasal endoscopic examination and magnetic resonance imaging (MRI)...
August 10, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28792825/the-world-of-wound-care
#16
Jackie Stephen-Haynes
No abstract text is available yet for this article.
August 10, 2017: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/28792354/death-anxiety-among-new-zealanders-the-predictive-roles-of-religion-spirituality-and-family-connection
#17
Rod MacLeod, Donna M Wilson, Jackie Crandall, Phil Austin
The aim of this cross-sectional study was to investigate attitudes of New Zealanders toward death and dying. We administered an online version of Collett-Lester Fear of Death Scale and Concerns about Dying Instrument subscales to a representative sample of the New Zealand population. One thousand one people responded to the survey, where the largest age-group lay between 30 and 39 years. Respondents with strong religious beliefs showed strongest agreement to being anxious about their own death compared to those who have no religious beliefs ( p = ...
January 1, 2017: Omega
https://www.readbyqxmd.com/read/28791769/fluorescent-polymer-nanoparticles-for-cell-barcoding-in-vitro-and-in-vivo
#18
Bohdan Andreiuk, Andreas Reisch, Marion Lindecker, Gautier Follain, Nadine Peyriéras, Jacky G Goetz, Andrey S Klymchenko
Fluorescent polymer nanoparticles for long-term labeling and tracking of living cells with any desired color code are developed. They are built from biodegradable poly(lactic-co-glycolic acid) polymer loaded with cyanine dyes (DiO, DiI, and DiD) with the help of bulky fluorinated counterions, which minimize aggregation-caused quenching. At the single particle level, these particles are ≈20-fold brighter than quantum dots of similar color. Due to their identical 40 nm size and surface properties, these nanoparticles are endocytosed equally well by living cells...
August 9, 2017: Small
https://www.readbyqxmd.com/read/28783757/improving-food-environments-and-tackling-obesity-a-realist-systematic-review-of-the-policy-success-of-regulatory-interventions-targeting-population-nutrition
#19
Jana Sisnowski, Jackie M Street, Tracy Merlin
BACKGROUND: This systematic review (PROSPERO: CRD42015025276) employs a realist approach to investigate the effect of "real-world" policies targeting different aspects of the food environment that shape individual and collective nutrition. OBJECTIVES: We were interested in assessing intermediate outcomes along the assumed causal pathway to "policy success", in addition to the final outcome of changed consumption patterns. DATA SOURCES: We performed a search of 16 databases through October 2015, with no initial restriction by language...
2017: PloS One
https://www.readbyqxmd.com/read/28777936/comparison-of-non-traditional-biomarkers-and-combinations-of-biomarkers-for-vascular-risk-prediction-in-people-with-type-2-diabetes-the-edinburgh-type-2-diabetes-study
#20
Anna H Price, Christopher J Weir, Paul Welsh, Stela McLachlan, Mark W J Strachan, Naveed Sattar, Jackie F Price
BACKGROUND AND AIMS: We aimed at comparing the impact of multiple non-traditional biomarkers (ankle brachial pressure index (ABI), N-terminal pro-brain natriuretic peptide (NT-proBNP), high sensitivity cardiac troponin (hs-cTnT), gamma-glutamyl transpeptidase (GGT) and four markers of systemic inflammation), both individually and in combination, on cardiovascular risk prediction, over and above traditional risk factors incorporated in the QRISK2 score, in older people with type 2 diabetes...
July 12, 2017: Atherosclerosis
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