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Zhe Zhang, Yue Zhang, Dong C Yang, Jin L Zhang
To investigate the degradation activity of the manganese ABC transporter, vegetative catalase 1 and acetoin dehydrogenase E1 from Bacillus subtilis YB1, the proteins were prokaryotically expressed and purified. Assay results showed that the three enzymes were able to degrade nicosulfuron (2- (4,6-dimethoxypyrimidine-2-pyrimidinylcarbamoylaminosulfonyl) -N,N-dimethylnicotinamide), with vegetative catalase 1 exhibiting the highest activity. To further examine the degradation pathway, the degradation products of the three enzymes and the YB1 strain were detected by liquid chromatography-mass spectrometry(LC-MS)...
March 29, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Iglika G Ivanova, Catherine V Park, Adrian I Yemm, Niall S Kenneth
HIF1α (hypoxia inducible factor 1α) is the central regulator of the cellular response to low oxygen and its activity is deregulated in multiple human pathologies. Consequently, given the importance of HIF signaling in disease, there is considerable interest in developing strategies to modulate HIF1α activity and down-stream signaling events. In the present study we find that under hypoxic conditions, activation of the PERK branch of the unfolded protein response (UPR) can suppress the levels and activity of HIF1α by preventing efficient HIF1α translation...
February 26, 2018: Nucleic Acids Research
E Zhiguo, Tingting Li, Huaya Zhang, Zehou Liu, Hui Deng, Sandeep Sharma, Xuefeng Wei, Lei Wang, Baixiao Niu, Chen Chen
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor that consists of three subunits, NF-YA, NF-YB, and NF-YC. Gene functions of NF-Ys during endosperm development are not well understood. In this study, we identified eight NF-Y encoding genes, including OsNF-YA8, OsNF-YB1,9, and OsNF-YC8,9,10,11,12, predominantly expressed in rice endosperm. Interestingly, the close homologs of these OsNF-Ys are present only in monocot species and preferentially expressed in endosperm as well, suggesting their roles in the regulation of endosperm development...
March 5, 2018: Journal of Experimental Botany
Brent M Kuenzi, Lily L Remsing Rix, Paul A Stewart, Bin Fang, Fumi Kinose, Annamarie T Bryant, Theresa A Boyle, John M Koomen, Eric B Haura, Uwe Rix
Targeted drugs are effective when they directly inhibit strong disease drivers, but only a small fraction of diseases feature defined actionable drivers. Alternatively, network-based approaches can uncover new therapeutic opportunities. Applying an integrated phenotypic screening, chemical and phosphoproteomics strategy, here we describe the anaplastic lymphoma kinase (ALK) inhibitor ceritinib as having activity across several ALK-negative lung cancer cell lines and identify new targets and network-wide signaling effects...
December 2017: Nature Chemical Biology
Jialin Wang, Sonja Djudjaj, Lydia Gibbert, Vera Lennartz, Daniel M Breitkopf, Thomas Rauen, Daniela Hermert, Ina V Martin, Peter Boor, Gerald S Braun, Jürgen Floege, Tammo Ostendorf, Ute Raffetseder
The Y-box-binding protein (YB)-1 plays a non-redundant role in both systemic and local inflammatory response. We analysed YB-1-mediated expression of the immune regulatory cytokine IL-10 in both LPS and sterile inflammation induced by unilateral renal ischaemia-reperfusion (I/R) and found an important role of YB-1 not only in the onset but also in the resolution of inflammation in kidneys. Within a decisive cis-regulatory region of the IL10 gene locus, the fourth intron, we identified and characterized an operative YB-1 binding site via gel shift experiments and reporter assays in immune and different renal cells...
December 2017: Journal of Cellular and Molecular Medicine
Yojiro Kotake, Nagisa Arikawa, Keiichiro Tahara, Hiroaki Maru, Madoka Naemura
BACKGROUND/AIM: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G1 phase of the cell cycle by controlling transcription of G1 regulators. Here, we report that YB1 is also involved in regulating G2/M phase. MATERIALS AND METHODS: YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immunoprecipitation (RIP)-chip assay was performed using anti-YB1 antibodies...
April 2017: Anticancer Research
Chang-Xian Li, Bin Yu, Lei Shi, Wei Geng, Qiu-Bin Lin, Chang-Chun Ling, Mei Yang, Kevin T P Ng, Jian-Dong Huang, Kwan Man
The antitumor properties of bacteria have been demonstrated over the past decades. However, the efficacy is limited and unclear. Furthermore, systemic infection remains a serious concern in bacteria treatment. In this study, the effect of YB1, a rationally designed 'obligate' anaerobic Salmonella typhimurium strain, on liver tumor growth and metastasis in a nude mouse orthotopic liver tumor model was investigated. The orthotopic liver tumor model was established in nude mice using the hepatocellular carcinoma cell line MHCC-97L...
January 2017: Oncology Letters
Jiahuang Li, Junfei Sun, Bin Wu, Bingfang He
To decrease d-Lactate production cost, wheat bran, a low-cost waste of milling industry, was selected as the sole feedstock. First, the nutrients were recovered from wheat bran by acid protease hydrolysis. Then, cellulosic hydrolysates were prepared from protease-treated samples after acid pretreatment and enzymatic saccharification. The combined use of nutrients and hydrolysates as nitrogen and carbon sources for fermentation by S. inulinus YB1-5 resulted in d-Lactate levels of 99.5g/L, with an average production efficiency of 1...
April 2017: Bioresource Technology
Jing-Jing Xu, Xiao-Fan Zhang, Hong-Wei Xue
Grain yield and quality of rice mainly depend on grain filling and endosperm development. Here we report that a rice NUCLEAR FACTOR Y (NF-Y) transcription factor, OsNF-YB1, is specifically expressed in the aleurone layer of developing endosperm and regulates grain filling and endosperm development. Knockdown of OsNF-YB1 expression by RNAi significantly retarded grain filling, leading to small grains with chalky endosperm as well as altered starch quality. Whereas OsNF-YB1 shows subcellular localization in both the cytosol and the nucleus in roots, it was specifically targeted to the nucleus of aleurone layer cells, which was facilitated by interacting with OsNF-YC proteins preferentially expressed in the aleurone layer...
December 2016: Journal of Experimental Botany
Alexander G Laman, Richard Lathe, Anna O Shepelyakovskaya, Alexandra Gartseva, Feodor A Brovko, Svetlana Guryanova, Ludmila Alekseeva, Elena A Meshcheryakova, Vadim T Ivanov
Bacterial cell wall muramyl dipeptide (MDP) and glucosaminyl-MDP (GMDP) are potent activators of innate immunity. Two receptor targets, NOD2 and YB1, have been reported; we investigated potential overlap of NOD2 and YB1 pathways. Separate knockdown of NOD2 and YB1 demonstrates that both contribute to GMDP induction of NF-κB expression, a marker of innate immunity, although excess YB1 led to induction in the absence of NOD2. YB1 and NOD2 co-migrated on sucrose gradient centrifugation, and GMDP addition led to the formation of higher molecular mass complexes containing both YB1 and NOD2...
November 2016: Innate Immunity
Yoshifumi Tokiwa, Boy Piening, Hirale S Jeevan, Sergey L Bud'ko, Paul C Canfield, Philipp Gegenwart
Low-temperature refrigeration is of crucial importance in fundamental research of condensed matter physics, because the investigations of fascinating quantum phenomena, such as superconductivity, superfluidity, and quantum criticality, often require refrigeration down to very low temperatures. Currently, cryogenic refrigerators with (3)He gas are widely used for cooling below 1 K. However, usage of the gas has been increasingly difficult because of the current worldwide shortage. Therefore, it is important to consider alternative methods of refrigeration...
September 2016: Science Advances
Jialin Wang, Lydia Gibbert, Sonja Djudjaj, Christina Alidousty, Thomas Rauen, Uta Kunter, Andreas Rembiak, Dieter Enders, Vera Jankowski, Gerald S Braun, Jürgen Floege, Tammo Ostendorf, Ute Raffetseder
Virtually all chronic kidney diseases progress towards tubulointerstitial fibrosis. In vitro, Y-box protein-1 (YB-1) acts as a central regulator of gene transcription and translation of several fibrosis-related genes. However, it remains to be determined whether its pro- or antifibrotic propensities prevail in disease. Therefore, we investigated the outcome of mice with half-maximal YB-1 expression in a model of renal fibrosis induced by unilateral ureteral obstruction. Yb1(+/-) animals displayed markedly reduced tubular injury, immune cell infiltration and renal fibrosis following ureteral obstruction...
December 2016: Kidney International
Hyejin Ryu, Milinda Abeykoon, Emil Bozin, Yosuke Matsumoto, S Nakatsuji, C Petrovic
We report on the evidence for the multiband electronic transport in α-YbAlB4 and α-Yb0.81(2)Sr0.19(3)AlB4. Multiband transport reveals itself below 10 K in both compounds via Hall effect measurements, whereas anisotropic magnetic ground state sets in below 3 K in α-Yb0.81(2)Sr0.19(3)AlB4. Our results show that Sr(2+) substitution enhances conductivity, but does not change the quasiparticle mass of bands induced by heavy fermion hybridization.
October 26, 2016: Journal of Physics. Condensed Matter: An Institute of Physics Journal
Serena De Negri, Vitaliy Romaka, Pavlo Solokha, Adriana Saccone, Gerald Giester, Herwig Michor, Peter F Rogl
Atom order in the crystal structures of Yb2Cu2-xMg (x = 0.17; Mo2FeB2-type; P4/mbm; a = 0.75592(2) nm; c = 0.40282(1) nm) and Yb9+xCuMg4-x (x = 0.034; Hf9Mo4B-type; P63/mmc; a = 1.0169(5) nm; c = 1.0290(5) nm) was determined from powder and X-ray single-crystal counter data analyses supported by electron probe microanalyses. Among the group of the so-called κ-phases, Yb9+xCuMg4-x is the first representative formed by a lanthanoid element. The structure of this κ-phase can be viewed as a typical network of corner-connected empty Yb6-octahedra, which encompass Yb6Mg6-icosahedra (filled by a mix of Mg/Yb atoms) and Yb6-trigonal prisms centered by Cu atoms to complete the three-dimensional metal framework...
August 15, 2016: Inorganic Chemistry
Bowie S K Chong, Evan G Moore
A series of lanthanide-based coordination polymers {[Yb1-xErx(4,4'-bpdo)3(H2O)2](CF3SO3)3}∞ were synthesised by solvent diffusion techniques, where 4,4'-bpdo = 4,4'-bipyridine-N,N'-dioxide, and using differing mole fractions of Yb(iii) and Er(iii) which were systematically varied (x = 0, 0.05, 0.20, 0.50 and 1). All of the materials obtained were characterised using elemental analyses, single-crystal X-ray diffraction (SXRD) and solid-state photoluminescence studies. Structurally, the coordination polymers crystallise as an isomorphous series of infinite 2D sheets, which contain two inner sphere water molecules, and are isostructural with a previously characterised homometallic Yb(iii) compound...
August 14, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Jian-Hong Shi, Nai-Peng Cui, Shuo Wang, Ming-Zhi Zhao, Bing Wang, Ya-Nan Wang, Bao-Ping Chen
Y-box-binding protein 1 (YB1) is a multifunctional transcription factor with vital roles in proliferation, differentiation and apoptosis. In this study, we have examined the role of its C-terminal domain (YB1 CTD) in proliferation, angiogenesis and tumorigenicity in breast cancer. Breast cancer cell line SK-BR-3 was infected with GFP-tagged YB1 CTD adenovirus expression vector. An 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) proliferation assay showed that YB1 CTD decreased SK-BR-3 cell proliferation, and down-regulated cyclin B1 and up-regulated p21 levels in SK-BR-3 cells...
January 2016: FEBS Open Bio
Dongliang Mo, Hongbo Fang, Kaifeng Niu, Jing Liu, Meng Wu, Shiyou Li, Tienian Zhu, Mohammed A Aleskandarany, Arvind Arora, Dileep N Lobo, Srinivasan Madhusudan, Adayabalam S Balajee, Zhenfen Chi, Yongliang Zhao
Elevation of the DNA-unwinding helicase RECQL4, which participates in various DNA repair pathways, has been suggested to contribute to the pathogenicity of various human cancers, including gastric cancer. In this study, we addressed the prognostic and chemotherapeutic significance of RECQL4 in human gastric cancer, which has yet to be determined. We observed significant increases in RECQL4 mRNA or protein in >70% of three independent sets of human gastric cancer specimens examined, relative to normal gastric tissues...
May 15, 2016: Cancer Research
A Dey, M Robitaille, M Remke, C Maier, A Malhotra, A Gregorieff, J L Wrana, M D Taylor, S Angers, A M Kenney
Postnatal proliferation of cerebellar granule neuron precursors (CGNPs), proposed cells of origin for the SHH-associated subgroup of medulloblastoma, is driven by Sonic hedgehog (Shh) and insulin-like growth factor (IGF) in the developing cerebellum. Shh induces the oncogene Yes-associated protein (YAP), which drives IGF2 expression in CGNPs and mouse Shh-associated medulloblastomas. To determine how IGF2 expression is regulated downstream of YAP, we carried out an unbiased screen for transcriptional regulators bound to IGF2 promoters...
August 11, 2016: Oncogene
Annika Osswald, Zhongke Sun, Verena Grimm, Grace Ampem, Karin Riegel, Astrid M Westendorf, Wolfgang Sommergruber, Kerstin Otte, Peter Dürre, Christian U Riedel
BACKGROUND: Several studies in animal models demonstrated that obligate and facultative anaerobic bacteria of the genera Bifidobacterium, Salmonella, or Clostridium specifically colonize solid tumors. Consequently, these and other bacteria are discussed as live vectors to deliver therapeutic genes to inhibit tumor growth. Therapeutic approaches for cancer treatment using anaerobic bacteria have been investigated in different mouse models. In the present study, solid three-dimensional (3D) multicellular tumor spheroids (MCTS) of the colorectal adenocarcinoma cell line HT-29 were generated and tested for their potential to study prodrug-converting enzyme therapies using bacterial vectors in vitro...
December 12, 2015: Microbial Cell Factories
Bin Yu, Lei Shi, Bao-Zhong Zhang, K E Zhang, Xiao Peng, Han-Ben Niu, Jun-LE Qu
The present authors have previously reported a novel approach to genetically engineer Salmonella typhimurium for the medically important therapeutic strategy of using bacterial agents to target malignant tumors in a breast cancer tumor-bearing nude mouse model. However, studying an immunocompromised mouse model for cancer therapy is insufficient, as certain crucial information about the influence of the immune system may be missing. In the present study, inoculation of the Salmonella strain, YB1, into a colon cancer tumor-bearing immunocompetent mouse model was investigated...
August 2015: Oncology Letters
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