Kim E Dzobo, Arjen Cupido, Barend M Mol, Lotte C A Stiekema, Miranda Versloot, Maaike Winkelmeijer, Jorge Peter, Anne-Marije Pennekamp, Stefan Havik, Frédéric M Vaz, Michel van Weeghel, Koen H M Prange, Johannes H M Levels, Menno P J de Winther, Sotirios Tsimikas, Albert K Groen, Erik S G Stroes, Dominique P V de Kleijn, Jeffrey Kroon
BACKGROUND: Oxidized phospholipids play a key role in the atherogenic potential of lipoprotein(a; Lp[a]); however, Lp(a) is a complex particle that warrants research into additional proinflammatory mediators. We hypothesized that additional Lp(a)-associated lipids contribute to the atherogenicity of Lp(a). METHODS: Untargeted lipidomics was performed on plasma and isolated lipoprotein fractions. The atherogenicity of the observed Lp(a)-associated lipids was tested ex vivo in primary human monocytes by RNA sequencing, ELISA, Western blot, and transendothelial migratory assays...
January 25, 2024: Arteriosclerosis, Thrombosis, and Vascular Biology