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Wouter C Meijers, A Rogier van der Velde, Anneke C Muller Kobold, Janneke Dijck-Brouwer, Alan H Wu, Allan Jaffe, Rudolf A de Boer
AIMS: Biomarkers can be used for diagnosis, risk stratification, or management of patients with heart failure (HF). Knowledge about the biological variation is needed for proper interpretation of serial measurements. Therefore, we aimed to determine and compare the biological variation of a large panel of biomarkers in healthy subjects and in patients with chronic HF. METHODS AND RESULTS: The biological variability of established biomarkers [NT-proBNP and high-sensitivity troponin T (hsTnT)], novel biomarkers [galectin-3, suppression of tumorigenicity 2 (ST2), and growth differentiation factor 15 (GDF-15)], and renal/neurohormonal biomarkers (aldosterone, phosphate, parathyroid hormone, plasma renin concentration, and creatinine) was determined in 28 healthy subjects and 83 HF patients, over a period of 4 months and 6 weeks, respectively...
October 21, 2016: European Journal of Heart Failure
Samseh Abdullah Noradilah, Ii Li Lee, Tengku Shahrul Anuar, Fatmah Md Salleh, Siti Nor Azreen Abdul Manap, Noor Shazleen Husnie Mohd Mohtar, Syed Muhamad Azrul, Wan Omar Abdullah, Norhayati Moktar
In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1-K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages...
2016: PeerJ
Monika Ryba-Stanisławowska, Paulina Werner, Maria Skrzypkowska, Agnieszka Brandt, Jolanta Myśliwska
IL-33 is an IL-1 cytokine family member, with ability to induce both Th1 and Th2 immune responses. It binds to ST2 receptor, whose deficiency is associated with enhanced inflammatory response. The most recent studies have shown the immunoregulatory effect of IL-33 on Tregs in animal models. As type 1 diabetes is an autoimmune, inflammatory disease, where Treg defects have been described, we aimed to analyze the in vitro influence of recombinant IL-33 on quantitative properties of regulatory CD4(+)CD25(high)FOXP3(+) T cells...
2016: Mediators of Inflammation
Jinggang Xia, Yang Qu, Chunlin Yin, Dong Xu
BACKGROUND: This study aimed to evaluate the role of β-blocker therapy on modulating interleukin (IL)-33/ST2 (interleukin-1 receptor-like 1) signaling during ventricular remodeling related to heart failure (HF) after acute myocardial infarction (AMI). METHODS: Sprague-Dawley rats that survived surgery to induce AMI were randomly divided into the placebo group and the β-blocker treatment group. A sham group was used as a control. Left ventricular (LV) function variables, the myocardial infarct size, fibrosis and IL-33/ST2 protein expression was measured...
October 17, 2016: Cardiology Journal
Sofia Theodoropoulou, David A Copland, Jian Liu, Jiahui Wu, Peter J Gardner, Ema Ozaki, Sarah L Doyle, Matthew Campbell, Andrew D Dick
Age-related macular degeneration (AMD) is the leading cause of central vision loss worldwide. Loss of retinal pigment epithelium (RPE) is a major pathological hallmark in AMD with or without pathological neovascularization. Although activation of the immune system is implicated in disease progression, pathological pathways remain diverse and unclear. Here, we report an unexpected protective role of a pro-inflammatory cytokine, interleukin-33 (IL-33) in ocular angiogenesis. IL-33 and its receptor (ST2) are expressed constitutively in human and murine retina and choroid...
October 4, 2016: Journal of Pathology
Masako Fujioka-Kobayashi, Benoit Schaller, Nikola Saulacic, Yufeng Zhang, Richard J Miron
Within the past years, BMP9 has been characterized as one of the most osteogenic bone-inducers among the BMP family, however up until recently, BMP9 has only been available through adenovirus transfection experiments(gene therapy) not approved for clinical use. The aim of this study was to investigate recombinant rhBMP9 versus rhBMP2 at 2 concentrations (10ng/mL and 100ng/mL) in combination with 2 bone grafts: 1)a natural bone mineral(NBM) without collagen versus 2)a novel NBM integrated with atelo-collagen type I(NBM-Col)...
October 3, 2016: Journal of Biomedical Materials Research. Part A
Erin D Gordon, Joe Palandra, Agata Wesolowska-Andersen, Lando Ringel, Cydney L Rios, Marrah E Lachowicz-Scroggins, Louis Z Sharp, Jamie L Everman, Hannah J MacLeod, Jae W Lee, Robert J Mason, Michael A Matthay, Richard T Sheldon, Michael C Peters, Karl H Nocka, John V Fahy, Max A Seibold
Genome-wide association studies of asthma have identified genetic variants in the IL1RL1 gene, but the molecular mechanisms conferring risk are unknown. IL1RL1 encodes the ST2 receptor (ST2L) for IL-33 and an inhibitory decoy receptor (sST2). IL-33 promotes type 2 inflammation, which is present in some but not all asthmatics. We find that two single nucleotide polymorphisms (SNPs) in IL1RL1 - rs1420101 and rs11685480 - are strongly associated with plasma sST2 levels, though neither is an expression quantitative trait locus (eQTL) in whole blood...
September 8, 2016: JCI Insight
Ralf Willebrand, David Voehringer
Eosinophils are major effector cells during allergic responses and helminth infections. Recent studies further highlight eosinophils as important players in many other biological processes. Therefore it is important to understand how these cells can be modulated in terms of survival and effector function. In the present study we investigated how eosinophils respond to the alarmin IL-33. We show that IL-33 promotes eosinophil survival in a ST2- and MyD88-dependent manner. IL-33-mediated protection from apoptosis was dependent on autocrine GM-CSF release...
2016: PloS One
Laura K Johnston, Chia-Lin Hsu, Rebecca A Krier-Burris, Krishan D Chhiba, Karen B Chien, Andrew McKenzie, Sergejs Berdnikovs, Paul J Bryce
Eosinophils are important in the pathogenesis of many diseases, including asthma, eosinophilic esophagitis, and eczema. Whereas IL-5 is crucial for supporting mature eosinophils (EoMs), the signals that support earlier eosinophil lineage events are less defined. The IL-33R, ST2, is expressed on several inflammatory cells, including eosinophils, and is best characterized for its role during the initiation of allergic responses in peripheral tissues. Recently, ST2 expression was described on hematopoietic progenitor subsets, where its function remains controversial...
September 28, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Wouter C Meijers, A Rogier van der Velde, Rudolf A de Boer
ST2 and galectin-3 are emerging biomarkers in the field of heart failure and have been extensively studied, and that whether they provide additional prognostic value on top of the clinical models and the gold standard in HF, (NT-pro)BNP. Our aim was to provide a comprehensive review of these emerging HF-related biomarkers in chronic, acute and incident heart failure. Regardless of the type of heart failure, both biomarkers seem to have an additional effect on top of the clinical model including natriuretic peptides...
August 2016: EJIFCC
Thomas Mueller, Benjamin Dieplinger
The proteins soluble ST2 (sST2) and galectin-3 are currently gaining mounting interest as candidate biomarkers in cardiac disease. Both, sST2 and galectin-3 have been included in the 2013 ACCF/AHA guideline for additive risk stratification of patients with acute and chronic heart failure. The aim of this review is to provide information on analytical considerations of measuring circulating sST2 and galectin-3 including knowledge on in vitro stability, biological variation and reference ranges of both analytes...
August 2016: EJIFCC
Mingyuan Han, Jun Young Hong, Suraj Jaipalli, Charu Rajput, Jing Lei, Joanna L Hinde, Qiang Chen, Natalie M Hershenson, J Kelley Bentley, Marc B Hershenson
Early-life wheezing-associated infections with rhinovirus (RV) have been associated with asthma development in children. We have shown that RV infection of six day-old mice induces mucous metaplasia and airways hyperresponsiveness which is dependent on IL-13, IL-25 and type 2 innate lymphoid cells (ILC2s). Infection of immature mice fails to induce lung IFN-γ expression, in contrast to mature 8 week-old mice with a robust IFN-γ response, consistent with the notion that deficient IFN-γ production in immature mice permits RV-induced type 2 immune responses...
September 28, 2016: American Journal of Respiratory Cell and Molecular Biology
Ayako Matsuki, Hiroaki Takatori, Sohei Makita, Masaya Yokota, Tomohiro Tamachi, Akira Suto, Kotaro Suzuki, Koichi Hirose, Hiroshi Nakajima
BACKGROUND: Innate lymphoid cells (ILCs) are emerging subsets of immune cells that produce large amounts of cytokines upon cytokine and/or alarmin stimulation. Recent studies have shown that T-bet plays pivotal roles in the development of ILC3s and ILC1s; however, the roles of T-bet in lung ILC2s remain unknown. OBJECTIVE: To determine the role of T-bet in ILC2-mediated airway inflammation. METHODS: The expression of T-bet in lung ILCs (defined as Thy1...
September 23, 2016: Journal of Allergy and Clinical Immunology
Ahmad Al Atrouni, Monzer Hamze, Tamima Jisr, Carole Lemarié, Matthieu Eveillard, Marie-Laure Joly-Guillou, Marie Kempf
OBJECTIVES: To investigate the molecular epidemiology of Acinetobacter baumannii strains isolated from different hospitals in Lebanon. METHODS: A total of 119 non-duplicate Acinetobacter strains were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and partial rpoB gene sequencing. Antibiotic susceptibility testing was performed by disc diffusion method and all identified carbapenem-resistant isolates were investigated by PCR assays for the presence of the carbapenemase-encoding genes...
September 20, 2016: International Journal of Infectious Diseases: IJID
Attakorn Palasuwan, Duangdao Palasuwan, Aongart Mahittikorn, Rachatawan Chiabchalard, Valery Combes, Supaluk Popruk
Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes...
August 2016: Korean Journal of Parasitology
Chunhong Wang, Zengsheng Chen, Xiangmao Bu, Yang Han, Shan Shan, Tao Ren, Weiqing Song
IL-33 is a member of IL-1 superfamily that drives production of Th2-related cytokines. Recently, accumulating evidence suggest an involvement of IL-33 in carcinogenesis. Herein, we determine a close correlation of IL-33 expression and cancer progress in patients with non-small-cell lung cancer (NSCLC). Overexpression of IL-33 by transfection with IL-33 expression vector enhances NSCLC outgrowth and metastasis, while genetic knockdown of IL-33 by transfection with IL-33 shRNA limits NSCLC progression. In consistent, IL-33 stimulation of NSCLC cells leads to robust NSCLC outgrowth and metastasis in vitro and in vivo...
October 21, 2016: Biochemical and Biophysical Research Communications
Lisa M Rice, Julio C Mantero, Giuseppina Stifano, Jessica Ziemek, Robert W Simms, Jessica Gordon, Robyn Domsic, Robert Lafyatis
In this study we systematically investigated alterations in the serum proteome of patients with diffuse cutaneous systemic sclerosis (dcSSc), and identified differentially expressed proteins that correlated with disease severity. Our goal was to identify a combination of serum proteins that would provide a biological measure for the extent of skin disease and that could be combined into a longitudinal pharmacodynamic biomarker. 16% of the sera proteins analyzed by SOMAscan aptamer technology, from two cohorts of dcSSc patients were identified as differentially regulated between dcSSc and controls, and correlated with the MRSS...
September 14, 2016: Journal of Investigative Dermatology
Adil Coskun, Erdogan Malatyali, Hatice Ertabaklar, Mustafa B Yasar, Ali O Karaoglu, Sema Ertug
OBJECTIVE: To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases. METHODS: Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct microscopy and cultivated in Jones medium. Blastocystis positive cultures were subjected to DNA isolation and subtypes were identified by sequencing of barcode region...
September 2016: Asian Pacific Journal of Tropical Medicine
Young-Dan Cho, Bong-Soo Kim, Chang-Seok Lee, Kyung-Hwa Kim, Yang-Jo Seol, Yong-Moo Lee, In-Chul Rhyu, Young Ku, Hyun-Mo Ryoo
BACKGROUND: In our previous studies, we demonstrated that a fibronectin (FN)-derived oligopeptide termed F20 stimulates osteoblast differentiation in vitro and bone formation in vivo. However, the fundamental molecular mechanism by which F20 stimulates osteogenesis remains unknown. Therefore, in this study we investigated the molecular mechanism underlying the effect of F20 in osteoblast differentiation. METHODS: The role of F20 in osteoblast differentiation was examined using the mouse bone-marrow-derived ST2 cell line...
September 13, 2016: Journal of Periodontology
Priyanka Sharma, Sushila Dahiya, Veeraraghavan Balaji, Anil Kanga, Preetilata Panda, Rashna Das, Anbumani Dhanraju, Deepak Kumar Mendiratta, Seema Sood, Bimal Kumar Das, Arti Kapil
Enteric fever is an invasive infection predominantly caused by Salmonella enterica serovars Typhi and Paratyphi A. The pathogens have evolved from other nontyphoidal salmonellaeto become invasive and host restricted. Emergence of antimicrobial resistance in typhoidal salmonellae in some countries is a major therapeutic concern as the travelers returning from endemic countries carry resistant strains to non endemic areas. In order to understand the epidemiology and to design disease control strategies molecular typing of the pathogen is very important...
2016: PloS One
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