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Yellow fever

Thomas Kröber, Konstantinos Koussis, Martine Bourquin, Panagiota Tsitoura, Maria Konstantopoulou, Taiwo Sam Awolola, Francesca R Dani, Huili Qiao, Paolo Pelosi, Kostas Iatrou, Patrick M Guerin
There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, with a predominantly female expression bias to identify plant essential oils (EOs) containing bioactive compounds that target mosquito olfactory function. EOs and their fractions capable of binding to such OBPs displayed repellence against female mosquitoes in a laboratory repellence assay...
April 12, 2018: Insect Biochemistry and Molecular Biology
Nicholas J Barrows, Rafael K Campos, Kuo-Chieh Liao, K Reddisiva Prasanth, Ruben Soto-Acosta, Shih-Chia Yeh, Geraldine Schott-Lerner, Julien Pompon, October M Sessions, Shelton S Bradrick, Mariano A Garcia-Blanco
Flaviviruses, such as dengue, Japanese encephalitis, tick-borne encephalitis, West Nile, yellow fever, and Zika viruses, are critically important human pathogens that sicken a staggeringly high number of humans every year. Most of these pathogens are transmitted by mosquitos, and not surprisingly, as the earth warms and human populations grow and move, their geographic reach is increasing. Flaviviruses are simple RNA-protein machines that carry out protein synthesis, genome replication, and virion packaging in close association with cellular lipid membranes...
April 13, 2018: Chemical Reviews
Eduardo Hermógenes Moretti, Jesús Eduardo Ortega Chinchilla, Fernando Silveira Marques, Pedro Augusto C M Fernandes, Fernando Ribeiro Gomes
Ectothermic vertebrates develop behavioral fever in response to bacterial products, with potential corresponding metabolic costs associated with immune stimulation. Although behavioral fever has been described in several taxa under laboratory conditions, some important questions regarding metabolic response to bacterial products at different temperatures and effectiveness of behavioral fever remain open. Many ectotherms, such as nocturnal anurans, may be active in the field at environmental conditions that restrict thermoregulation during the immune response...
April 9, 2018: Physiology & Behavior
Holly R Hughes, Brandy J Russell, Eric C Mossel, John Kayiwa, Julius Lutwama, Amy J Lambert
Yellow fever (YF) is a reemerging public health threat with frequent outbreaks prompting large vaccination campaigns in endemic regions of Africa and South America. Specific detection of vaccine-related adverse events is resource-intensive, time-consuming and difficult to achieve during an outbreak. To address this, we have developed a highly transferable, rapid yellow fever virus (YFV) vaccine-specific real-time RT-PCR assay that distinguishes vaccine from wild-type lineages. The assay utilizes a specific hydrolysis probe that includes locked nucleic acids to enhance specific discrimination of the YFV 17D vaccine strain genome...
April 11, 2018: Journal of Clinical Microbiology
Christian E Gunning, Kenichi Okamoto, Helvio Astete, Gissella M Vasquez, Erik Erhardt, Clara Del Aguila, Raul Pinedo, Roldan Cardenas, Carlos Pacheco, Enrique Chalco, Hugo Rodriguez-Ferruci, Thomas W Scott, Alun L Lloyd, Fred Gould, Amy C Morrison
BACKGROUND: Aedes aegypti is a primary vector of dengue, chikungunya, Zika, and urban yellow fever viruses. Indoor, ultra low volume (ULV) space spraying with pyrethroid insecticides is the main approach used for Ae. aegypti emergency control in many countries. Given the widespread use of this method, the lack of large-scale experiments or detailed evaluations of municipal spray programs is problematic. METHODOLOGY/PRINCIPAL FINDINGS: Two experimental evaluations of non-residual, indoor ULV pyrethroid spraying were conducted in Iquitos, Peru...
April 6, 2018: PLoS Neglected Tropical Diseases
Felipe Gomes Naveca, Valdinete Alves Nascimento, Victor Costa Souza, Regina M P de Figueiredo
Introduction: Several orthobunyaviruses are important arthropod-borne pathogens, responsible for a variety of diseases in humans, from acute febrile illness to encephalitis. Methods: We collected serum samples from a series of dengue suspected cases in Tefé, a mid-size city located in the interior of the Amazonas state, Brazil. Viral RNA extraction was performed, and specimens were tested for dengue virus using RT-PCR. Thirty dengue negative samples were further tested for Mayaro virus (MAYV) and Oropouche virus (OROV) using an RT-qPCR protocol previously described...
March 22, 2018: PLoS Currents
Robert J Meagher, Aashish Priye, Yooli K Light, Cheng Huang, Eryu Wang
Loop-mediated isothermal amplification (LAMP), coupled with reverse transcription (RT), has become a popular technique for detection of viral RNA due to several desirable characteristics for use in point-of-care or low-resource settings. The large number of primers in LAMP (six per target) leads to an increased likelihood of primer dimer interactions, and the inner primers in particular are prone to formation of stable hairpin structures due to their length (typically 40-45 bases). Although primer dimers and hairpin structures are known features to avoid in nucleic acid amplification techniques, there is little quantitative information in literature regarding the impact of these structures on LAMP or RT-LAMP assays...
April 5, 2018: Analyst
Hercules Sakkas, Petros Bozidis, Ashley Franks, Chrissanthy Papadopoulou
Oropouche fever is an emerging zoonotic disease caused by Oropouche virus (OROV), an arthropod transmitted Orthobunyavirus circulating in South and Central America. During the last 60 years, more than 30 epidemics and over half a million clinical cases attributed to OROV infection have been reported in Brazil, Peru, Panama, Trinidad and Tobago. OROV fever is considered the second most frequent arboviral febrile disease in Brazil after dengue fever. OROV is transmitted through both urban and sylvatic transmission cycles, with the primary vector in the urban cycle being the anthropophilic biting midge Culicoides paraensis ...
April 4, 2018: Viruses
Vanesa Rodríguez Fernández, Carlos Nicolás López Ramón Y Cajal, Elena Marín Ortiz, Emilio Couceiro Naveira
OBJECTIVE: To determine the intrapartum and perinatal results associated with different degrees of staining of meconium stained amniotic fluid (MSAF). STUDY DESIGN: In a retrospective cohort study of all singleton deliveries over a period of one year (2015) in a tertiary hospital, we compared different degrees of MSAF (yellow, green and thick) to clear amniotic fluids, and analysed in each group maternal, intrapartum and neonatal variables as well as umbilical cord blood gas analysis...
March 19, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Alejandra Rojas, Cheikh T Diagne, Victoria D Stittleburg, Alisha Mohamed-Hadley, Yvalena Arévalo de Guillén, Angel Balmaseda, Oumar Faye, Ousmane Faye, Amadou A Sall, Eva Harris, Benjamin A Pinsky, Jesse J Waggoner
The differential diagnosis of dengue virus (DENV) and yellow fever virus (YFV) infections in endemic areas is complicated by nonspecific early clinical manifestations. In this study, we describe an internally controlled, multiplex real-time reverse transcription PCR (rRT-PCR) for the detection of DENV and YFV. The DENV-YFV assay demonstrated specific detection and had a dynamic range of 2.0-8.0 log10 copies/μL of eluate for each DENV serotype and YFV. Clinical performance was similar to a published pan-DENV assay: 48/48 acute-phase samples from dengue cases were detected in both assays...
April 2, 2018: American Journal of Tropical Medicine and Hygiene
Raphaëlle Klitting, Laura Roth, Félix A Rey, Xavier de Lamballerie
Yellow fever virus (Flavivirus genus) is an arthropod-borne pathogen, which can infect humans, causing a severe viscerotropic disease with a high mortality rate. Adapted viral strains allow the reproduction of yellow fever disease in hamsters with features similar to the human disease. Here, we used the Infectious Subgenomic Amplicons reverse genetics method to produce an equivalent to the hamster-virulent strain, Yellow Fever Ap7, by introducing a set of four synonymous and six nonsynonymous mutations into a single subgenomic amplicon, derived from the sequence of the Asibi strain...
March 29, 2018: Emerging Microbes & Infections
Lin H Chen, Davidson H Hamer
No abstract text is available yet for this article.
March 27, 2018: JAMA: the Journal of the American Medical Association
Scott A Norton, David M Morens
No abstract text is available yet for this article.
March 27, 2018: JAMA: the Journal of the American Medical Association
Andressa de Oliveira Aragão, Joaquim Pinto Nunes Neto, Ana Cecília Ribeiro Cruz, Samir Mansour Moraes Casseb, Jedson Ferreira Cardoso, Sandro Patroca da Silva, Edna Aoba Yassui Ishikawa
Mosquitoes as Sabethes chloropterus, Sabethes glaucodaemon, Sabethes belisarioi are species of medical and epidemiological importance for arboviruses transmission such as yellow fever and St. Louis encephalitis. Despite this, no information about these three species mitochondrial DNA has been found in literature. Our study presents a mitochondrial genome description, including identity, SNPs, mutation rate, and phylogeny analysis using COX1, COX2, NADH4, NADH5, CYOB genes. The Sa. chloropterus, Sa. glaucodaemon and Sa...
March 23, 2018: Genomics
Anaelle da Costa, Christophe Prehaud, Cecile Khou, Nathalie Pardigon, Aure Saulnier, Nolwenn Nougarede, Monique Lafon
Live attenuated vaccines have proved to be mostly valuable in the prevention of infectious diseases in humans, especially in developing countries. The safety and potency of vaccine, and the consistency of vaccine batch-to-batch manufacturing, must be proven before being administrated to humans. For now, the tests used to control vaccine safety largely involve animal testing. For live viral vaccines, regulations require suppliers to demonstrate the absence of neurovirulence in animals, principally in non-human primates and mice...
March 23, 2018: Biologicals: Journal of the International Association of Biological Standardization
Huiqiang Yang, Huan Yang, Zhushi Li, Lina Liu, Wei Wang, Ting He, Fengming Fan, Yan Sun, Jie Liu, Yuhua Li, Xianwu Zeng
Yellow fever (YF) is an acute viral haemorrhagic disease caused by the yellow fever virus (YFV), which remains a potential threat to public health. The live-attenuated YF vaccine (17D strain) is a safe and highly effective measure against YF. However, increasing adverse events have been associated with YF vaccinations in recent years; thus, safer, alternative vaccines are needed. In this study, using the Japanese encephalitis live vaccine strain SA14-14-2 as a backbone, a novel chimeric virus was constructed by replacing the pre-membrane (prM) and envelope (E) genes with their YFV 17D counterparts...
March 23, 2018: Vaccine
Shavonn R Whiten, W Keith Ray, Richard F Helm, Zach N Adelman
The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses must transverse the midgut to replicate, the blood meal must also reach the midgut to be digested, absorbed, or excreted, as aggregation of blood meal metabolites can be toxic to the female mosquito midgut. The midgut peritrophic matrix (PM), a semipermeable extracellular layer comprised of chitin fibrils, glycoproteins, and proteoglycans, is one such mechanism of protection for the mosquito midgut...
2018: PloS One
Eduardo J M Nascimento, James K George, Melissa Velasco, Matthew I Bonaparte, Lingyi Zheng, Carlos DiazGranados, Ernesto T A Marques, James W Huleatt
Dengue virus infection elicits immune responses to multiple viral antigens including antibodies to dengue non-structural protein 1 (NS1) which are rapidly induced and detected within days of infection. The recombinant, live, attenuated, tetravalent dengue vaccine (CYD-TDV; Sanofi Pasteur) uses the yellow fever vaccine virus as a back-bone but expresses dengue virus pre-membrane and envelop proteins. Since CYD-TDV does not express dengue NS1, we evaluated the utility of dengue NS1-specific IgG antibodies as biomarkers of dengue exposure in CYD-TDV recipients and controls...
March 19, 2018: Journal of Virological Methods
Davidson H Hamer, Kristina Angelo, Eric Caumes, Perry J J van Genderen, Simin A Florescu, Corneliu P Popescu, Cecilia Perret, Angela McBride, Anna Checkley, Jenny Ryan, Martin Cetron, Patricia Schlagenhauf
Yellow fever virus is a mosquito-borne flavivirus that causes yellow fever, an acute infectious disease that occurs in South America and sub-Saharan Africa. Most patients with yellow fever are asymptomatic, but among the 15% who develop severe illness, the case fatality rate is 20%-60%. Effective live-attenuated virus vaccines are available that protect against yellow fever (1). An outbreak of yellow fever began in Brazil in December 2016; since July 2017, cases in both humans and nonhuman primates have been reported from the states of São Paulo, Minas Gerais, and Rio de Janeiro, including cases occurring near large urban centers in these states (2)...
March 23, 2018: MMWR. Morbidity and Mortality Weekly Report
Danielle Menosi Gualandro, Nancy Bellei, Mucio Tavares de Oliveira, Sérgio Montenegro
No abstract text is available yet for this article.
January 2018: Revista da Associação Médica Brasileira
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