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Olesya Yu Shoeva
The wheat TaMyc1 gene encodes for transcriptional factor (TF) with bHLH domain. The gene is expressed in purple wheat grains and activates transcription of the anthocyanin biosynthesis structural genes. To reveal the features of TaMyc1 regulation in wheat pericarp transcription start sites (TSS) were identified by 5' RACE mean and translation efficiency was predicted by in silico methods. Three alternative transcript variants of TaMyc1 differing by 5' leader sequence only were identified in purple pericarp...
March 19, 2018: Molecular Biology Reports
Andrew Niedringhaus, Veronica R Policht, Riley Sechrist, Arkaprabha Konar, Philip D Laible, David F Bocian, Dewey Holten, Christine Kirmaier, Jennifer P Ogilvie
In the initial steps of photosynthesis, reaction centers convert solar energy to stable charge-separated states with near-unity quantum efficiency. The reaction center from purple bacteria remains an important model system for probing the structure-function relationship and understanding mechanisms of photosynthetic charge separation. Here we perform 2D electronic spectroscopy (2DES) on bacterial reaction centers (BRCs) from two mutants of the purple bacterium Rhodobacter capsulatus , spanning the Q y absorption bands of the BRC...
March 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
Lionel Rebibo, Abdennaceur Dhahri, Brice Robert, Jean-Marc Regimbeau
BACKGROUND: Few series are available concerning repeat sleeve gastrectomy (re-SG), and series have reported contradictory results concerning morbidity rates, with limited data concerning weight loss. OBJECTIVE: Evaluate the short- and medium-term outcomes of re-SG. SETTING: University hospital, France, public practice. METHODS: Between June 2007 and March 2016, all patients undergoing re-SG (n = 46 patients) were included...
April 2018: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
Jing Li, Yong-Jun He, Lu Zhou, Yang Liu, Mingmin Jiang, Li Ren, Huoying Chen
BACKGROUND: The anthocyanins are highly enriched in eggplants (Solanum melongena L.) with purple peel. However, our previous study showed that anthocyanins biosynthesis in eggplant cultivar 'Lanshan Hexian' was completely regulated by light and color becomes evident at most 2 days after exposure to light. In the present investigation, transcriptome study was made to explore the underlying molecular mechanisms of light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L...
March 20, 2018: BMC Genomics
Jian Zha, Mattheos A G Koffas
Microbial production of plant-derived natural products by engineered microorganisms has achieved great success thanks to large extend to metabolic engineering and synthetic biology. Anthocyanins, the water-soluble colored pigments found in terrestrial plants that are responsible for the red, blue and purple coloration of many flowers and fruits, are extensively used in food and cosmetics industry; however, their current supply heavily relies on complex extraction from plant-based materials. A promising alternative is their sustainable production in metabolically engineered microbes...
December 2017: Synthetic and Systems Biotechnology
Wenji Xu, Gangjun Luo, Fengyang Yu, Qingxiang Jia, Yang Zheng, Xiaoying Bi, Jiajun Lei
Iris dichotoma with different flower colors and I. domestica are beardless wild irises belonging to the family Iridaceae that bloom in the summer and have long flowering periods. In this study, we collected three accessions of I. dichotoma with violet, yellow, and white flowers, respectively, in China, and crossed them with I. domestica individuals. The flower color of the hybrids derived from these crosses was categorized into eight groups: violet, purple, brown, orange, red, pink, yellow, and white. From this population, 45 individuals were selected for analysis, and their fully expanded inner and outer perianths were harvested for extraction of anthocyanins...
March 15, 2018: Phytochemistry
El-Sayed M Abdel-Aal, Pierre Hucl, Iwona Rabalski
Demands for anthocyanin-rich ingredients are steadily growing due to their positive functions in human health and food coloration. Commercially available purple wheat was evaluated as a source of functional ingredients based on anthocyanin composition and antioxidant properties. Anthocyanins were concentrated in the bran fraction by about 2-fold and then the bran was further processed into anthocyanin-rich powder using ethanol extraction and column purification. Batch studies showed that anthocyanin concentration factor increased by about 81- to 135-fold in the powder depending on batch size...
July 15, 2018: Food Chemistry
Glaucia Resende Soares, Carolina Foot Gomes de Moura, Marcelo Jose Dias Silva, Wagner Vilegas, Aline Boveto Santamarina, Luciana Pellegrini Pisani, Debora Estadella, Daniel Araki Ribeiro
The aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). For this purpose, histopathological analysis, proliferative status, antioxidant activity and inflammatory status were investigated in this setting. A total of 20 male rats were distributed into four groups as follows (n = 5 per group): Group 1-free access to water and commercial diet for 12 weeks; Group 2-received 4NQO at 50 ppm dose in drinking water daily and commercial diet for 12 weeks; Group 3-free access to water and received diet supplemented with purple carrot extract (0...
March 15, 2018: Medical Oncology
Elizabeth A MacDonald, Eric Donovan, Yukitoshi Nishimura, Nathan A Case, D Megan Gillies, Bea Gallardo-Lacourt, William E Archer, Emma L Spanswick, Notanee Bourassa, Martin Connors, Matthew Heavner, Brian Jackel, Burcu Kosar, David J Knudsen, Chris Ratzlaff, Ian Schofield
A glowing ribbon of purple light running east-west in the night sky has recently been observed by citizen scientists. This narrow, subauroral, visible structure, distinct from the traditional auroral oval, was largely undocumented in the scientific literature and little was known about its formation. Amateur photo sequences showed colors distinctly different from common types of aurora and occasionally indicated magnetic field-aligned substructures. Observations from the Swarm satellite as it crossed the arc have revealed an unusual level of electron temperature enhancement and density depletion, along with a strong westward ion flow, indicating that a pronounced subauroral ion drift (SAID) is associated with this structure...
March 2018: Science Advances
Ankita Jagannath Lakade, Kothandapani Sundar, Prathapkumar H Shetty
A new approach was developed for a simple and easy colorimetric detection assay to detect the use of calcium carbide in artificial ripening of fruits. Residues of arsenic on the fruit surface were used as an indicator for this. Use of calcium carbide in artificial ripening has been banned in many countries including India. In the present study, we have used a gold nanoparticle-based colorimetric detection method for determination of artificial ripening of fruits. ICP-MS analysis showed the presence of higher amounts of arsenic on fruits ripened using calcium carbide...
March 15, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Nanako Kanno, Katsumi Matsuura, Shin Haruta
Purple photosynthetic bacteria utilize light energy for growth. We previously demonstrated that light energy contributed to prolonging the survival of multiple purple bacteria under carbon-starved conditions. In order to clarify the effects of illumination on metabolic states under carbon-starved, non-growing conditions, we herein compared the metabolic profiles of starved cells in the light and dark using the purple bacterium, Rhodopseudomonas palustris. The metabolic profiles of starved cells in the light were markedly different from those in the dark...
March 13, 2018: Microbes and Environments
Hee Kyoung Joo, Sunga Choi, Yu Ran Lee, Eun Ok Lee, Myoung Soo Park, Kyu Been Park, Cuk-Seong Kim, Yong Pyo Lim, Jong-Tae Park, Byeong Hwa Jeon
Anthocyanins, the most prevalent flavonoids in red/purple fruits and vegetables, are known to improve immune responses and reduce chronic disease risks. In this study, the anti-inflammatory activities of an anthocyanin-rich extract from red Chinese cabbage (ArCC) were shown based on its inhibitory effects in cultured endothelial cells and hyperlipidemic apolipoprotein E -deficient mice. ArCC treatment suppressed monocyte adhesion to tumor necrosis factor-α-stimulated endothelial cells. This was validated by ArCC's ability to downregulate the expression and transcription of endothelial adhesion molecules, determined by immunoblot and luciferase promoter assays, respectively...
March 12, 2018: International Journal of Molecular Sciences
Daniel Montemayor, Eva Rivera, Seogjoo J Jang
Light harvesting 2 (LH2) complex is a primary component of the photosynthetic unit of purple bacteria that is responsible for harvesting and relaying excitons. The electronic absorption line shape of LH2 contains two major bands at 800 nm and 850 nm wavelength regions. Under low light condition, some species of purple bacteria replace LH2 with LH3, a variant form with almost the same structure as the former but with distinctively different spectral features. The major difference between the absorption line shapes of LH2 and LH3 is the shift of the 850 nm band of the former to a new 820 nm region...
March 13, 2018: Journal of Physical Chemistry. B
Alexandra Yannoutsos, Julie Malloizel-Delaunay, Emilie Tournier, Abdelaziz Abid, Alessandra Bura-Rivière
In this observation, we report an unusual presentation of a pilomatricoma in an 8-year-old girl who was initially referred to the department of vascular medicine for diagnosis and care of a suspected mixed lymphatic venous malformation. The lesion on her left shoulder presented as a giant bluish-purple red solitary mass, painful and rapidly growing, measuring 7 cm in anteroposterior diameter. This mass did not present the typical characteristics of a lymphatic venous malformation but exhibited warning signs of malignancy on clinical examination and imaging...
February 27, 2018: American Journal of Dermatopathology
Hyemin Kim, Nagahiro Saito
MnO2 -carbon hybrid (MnO2 -C-PBz) was simultaneously synthesized by a one-step solution plasma process (SPP) using a single precursor referred to as "purple benzene", which was derived from the K+ (dicyclohexano-18-crown-6 ether) complex. To clarify the synergistic effects on the cationic dye removal, MnO2 -free carbon and carbon-free MnO2 samples were concurrently investigated. The results of adsorption for cationic dyes (methylene blue (MB) and rhodamine B (Rh B)) and anionic dye (methyl orange (MO)) revealed remarkably high affinity for cationic dyes...
March 12, 2018: Scientific Reports
Muhe Diao, Jef Huisman, Gerard Muyzer
Sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB) drive major transformations in the sulfur cycle, and play vital roles in oxic-anoxic transitions in lakes and coastal waters. However, information on the succession of these sulfur bacteria in seasonally stratified lakes using molecular biological techniques is scarce. Here, we used 16S rRNA gene amplicon sequencing to study the spatio-temporal dynamics of sulfur bacteria during oxic-anoxic regime shifts in Lake Vechten. Oxygen and sulfate were mixed throughout the water column in winter and early spring...
March 8, 2018: FEMS Microbiology Ecology
Kakharman Yesmembetov, Natalya Satlikova, Zhanat Spatayev, Kulpash Kaliaskarova
We present a 21-year-old patient, remarkable for huge hepatomegaly with the liver, occupying almost the entire abdominal cavity, and mild portal hypertension due to splenic vein compression. After ultrasonography-guided liver biopsy, performed to establish the diagnosis, the patient had bleeding from the liver. Fortunately, emergency laparotomy was started immediately, and the patient was saved. Macroscopically, the liver appeared to be of purple-red color, flabby to the touch, and able to be easily wrinkled with fingers...
March 2018: Experimental and Clinical Transplantation
Minhui He, Qiong Hu, Yaqi Mei, Baojing Zhou, Jinming Kong, Xueji Zhang
The ability to directly detect alkaline phosphatase (ALP) activity in undiluted serum samples is of great importance for clinical diagnosis. In this work, we report the use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the Cu(BCA)2 + (BCA = bicinchoninic acid) reporter for the visual detection of ALP activity. In the presence of ALP, the substrate ascorbic acid 2-phosphate (AAP) can be enzymatically hydrolyzed to release ascorbic acid (AA), which in turn reduces Cu2+ to Cu+ ...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Sydney Moser, Ingrid Aragon, Amber Furrer, Jan-Willem Van Klinken, Melissa Kaczmarczyk, Byung-Hoo Lee, Judy George, Bruce R Hamaker, Richard Mattes, Mario G Ferruzzi
Beneficial effects of some phenolic compounds in modulation of carbohydrate digestion and glycemic response have been reported, however effects of phenolics from processed potato products on these endpoints are not well known. The aims of this study were to characterize phenolic profiles of fresh potatoes (purple, red, or white fleshed; 2 varieties each) and chips, and to examine the potential for potato phenolic extracts (PPE) to modulate starch digestion and intestinal glucose transport in model systems. Following in vitro assessment, a pilot clinical study (n=11) assessed differences in glycemic response and gastric emptying between chips from pigmented and white potatoes...
February 12, 2018: Nutrition Research
Sandra M Londoño-Restrepo, Natalia Rincón-Londoño, Margarita Contreras-Padilla, Beatriz M Millan-Malo, Mario E Rodriguez-Garcia
This work is focused on the chemical, structural, morphological, thermal, IR vibrational, and pasting characterization of isolated white, yellow, and purple Arracacha starches from Colombia. Inductive couple plasma showed that these starches are rich in potassium. Scanning Electron Microscopy (SEM) images show that the starch granules are formed by ovoid fully filled Lego-like starch microparticles, the circular cross-section has a diameter between 9 and 15 μm and mayor axis between 20 and 30 μm. Each one of these ovoids is formed by irregular wedge-shaped 6 to 10 isolated starch granules with an average size between 4 and 12 μm...
March 6, 2018: International Journal of Biological Macromolecules
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