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Yanhong Li, Xiaolong Sui, Hua Zhu, Yanfeng Xu, Lan Huang, Yuhuan Xu, Yunlin Han, Xiaochun Feng, Chuan Qin
Henoch-Schönlein purpura (HSP) is a systemic vasculitis mediated by autologous immune complex. Animal models of HSP are scarce. Here, we describe the characteristics of HSP rabbit model in the acute and recovery phase. First, we constructed the HSP rabbit models, and then assessed immunologic indicators of models by enzyme-linked immunosorbent assay and immunoturbidimetry. Histomorphological characteristics were analyzed by haematoxylin-eosin, immunofluorescence and special staining. In the acute stage (24 h) after antigen challenge, the model group rabbits featured skin ecchymosis and abnormal laboratory examination results...
October 21, 2016: Archives of Dermatological Research
Yanan Zhang, Loong-Tak Lim
Colorimetric CO2 indicators were prepared by printing pH dyes (M-cresol purple or phenol red, tetrabutylammonium hydroxide, ethylcellulose, 1:2 ethanol:1-butanol solvent), using a commercially available thermal inkjet printer, on either cellulose paper or plastic transparency film. Indicators inks printed on the papers had higher sensitivity to CO2, greater color change, and more producible than those printed on plastic film. Varying the print intensities of the two pH dyes resulted in multiplexed indicators with tunable sensitivity and expanded detection concentration range towards CO2...
December 1, 2016: Talanta
Jiajia Li, Chunlai Ding, Zhengbiao Zhang, Xiangqiang Pan, Na Li, Jian Zhu, Xiulin Zhu
Butyl acrylate is polymerized in the living way under the irradiation of purple light-emitting diode (LED) or sunlight without photocatalyst at ambient temperature. 2-((Phenoxycarbonothioyl)thio) ethyl propanoate) is exclusively added and acted as an initiator and a chain transfer agent simultaneously in the current system. Poly(butyl acrylate) with well-regulated molecular weight and relatively narrow molecular weight distribution (Ð < 1.30) is synthesized. High conversion (>95%) can be achieved within several minutes...
October 21, 2016: Macromolecular Rapid Communications
Nahid Alimoradi, Mohammad Reza Ashrafi-Kooshk, Mohsen Shahlaei, Shabnam Maghsoudi, Hadi Adibi, Ross P McGeary, Reza Khodarahmi
Purple acid phosphatases (PAPs) are binuclear metallo-hydrolases that have been isolated from various mammals, plants, fungi and bacteria. In mammals, PAP activity is associated with bone resorption and can lead to bone metabolic disorders such as osteoporosis; thus human PAP is an attractive target to develop anti-osteoporotic drugs. The aim of the present study was to investigate inhibitory effect of synthesized diethylalkylsulfonamido(4-methoxyphenyl)methyl)phosphonate/phosphonic acid derivatives as potential red kidney bean PAP (rkbPAP) inhibitors accompanied by experimental and molecular modeling assessments...
October 21, 2016: Journal of Enzyme Inhibition and Medicinal Chemistry
Chengchen Li, Caifeng Li, Haiyan Zhang, Hong Liao, Xiurong Wang
Induction of secreted and intracellular purple acid phosphatases (PAPs; EC is widely recognized as an adaptation of plants to phosphorus (P) deficiency. The secretion of PAPs plays important roles in P acquisition. However, little is known about the functions of intracellular PAP in plants and nodules. In this work, we identified a novel PAP gene GmPAP21 in soybean. Expression of GmPAP21 was induced by P limitation in nodules, roots and old leaves, and increased in roots with increasing duration of P starvation...
October 20, 2016: Physiologia Plantarum
B Lozano-Masdemont, L Gómez-Recuero-Muñoz, A Pulido-Pérez, I Molina-López, R Suárez-Fernández
Hepatitis B virus (HBV) infection in pregnant women is very rare in western countries, thus, cutaneous manifestation of HBV infection may be confused with a dermatosis specific of pregnancy. We report a 39-year-old woman who presented in her 20th week of pregnancy with a pruritic rash, which consisted of generalized erythematous plaques, some of them with a purple centre. Serology testing showed acute HBV infection, and a biopsy revealed a superficial and interstitial perivascular inflammatory infiltrate of lymphocytes and eosinophils...
October 20, 2016: Clinical and Experimental Dermatology
Lothar Esser, Fei Zhou, Yihui Zhou, Yumei Xiao, Wai-Kwan Tang, Chang-An Yu, Zhaohai Qin, Di Xia
The Complex III or cytochrome bc1 (cyt bc1) complex constitutes an integral part of the respiratory chain of most aerobic organisms and of the photosynthetic apparatus of anoxygenic purple bacteria. The function of cyt bc1 is to couple the reaction of electron transfer (ET) from ubiquinol to cytochrome c to proton pumping across the membrane. Mechanistically, the ET reaction requires docking of its Rieske iron-sulfur protein (ISP) subunit to the quinol oxidation (QP) site of the complex. Formation of an H-bond between the ISP and the bound substrate was proposed to mediate the docking...
October 7, 2016: Journal of Biological Chemistry
Nikolai N Sluchanko, Konstantin E Klementiev, Evgeny A Shirshin, Georgy V Tsoraev, Thomas Friedrich, Eugene G Maksimov
In Cyanobacteria, the Orange Carotenoid Protein (OCP) and Fluorescence Recovery Protein (FRP) are central to the photoprotective mechanism consisting in regulated quenching of phycobilisome (PBs) fluorescence. Due to a transient and flexible nature of the light-activated red quenching form, OCP(R), which is obtained from the stable dark-adapted orange form, OCP(O), by photoconversion, the detailed mechanism of photoprotection remains unclear. Here we demonstrate that our recently described W288A mutant of the Synechocystis OCP (hereinafter called OCP(W288A)) is a fully functional analogue of the OCP(R) form which is capable of constitutive PBs fluorescence quenching in vitro with no need of photoactivation...
October 15, 2016: Biochimica et Biophysica Acta
Adam E Brummett, Mishtu Dey
The marine microbial catabolism of dimethylsulfoniopropionate (DMSP) by the lyase pathway liberates ~300 million tons of dimethylsulfide (DMS) per year, which plays a major role in the biogeochemical cycling of sulfur. Recent biochemical and structural studies of some DMSP lyases, including DddQ reveal the importance of divalent transition metal ions in assisting DMSP cleavage. While DddQ is believed to be zinc-dependent primarily based on structural studies, excess zinc inhibits the enzyme. We examine the importance of iron in regulating the DMSP β-elimination reaction catalyzed by DddQ as our as-isolated purple-colored enzyme possesses ~0...
October 18, 2016: Biochemistry
Airek R Mathews, Shane S Que Hee
The differences in permeation parameters when a gloved dextrous robot hand clenched and did not were investigated with the dynamic permeation system described in the companion paper. Increased permeation through the gloves of the present study for cyclohexanol when the gloved hand clenched depended on glove thickness and porosity for cyclohexanol permeation. The Sterling glove, the thinnest and the most porous, was the least protective. Hand clenching promoted more permeation for the Sterling glove in terms of breakthrough times, steady state permeation rate, and diffusion coefficient...
October 18, 2016: Journal of Occupational and Environmental Hygiene
Airek R Mathews, Shane S Que Hee
The aim was to develop a whole glove permeation method for cyclohexanol to generate permeation parameter data for a non-moving dextrous robot hand (normalized breakthrough time tb, standardized breakthrough time ts, steady state permeation rate Ps, and diffusion coefficient D). Four types of disposable powderless, unsupported and unlined nitrile gloves from the same producer were investigated: Safeskin Blue, and Kimtech Science Blue, Purple, and Sterling. The whole glove method developed involved a peristaltic pump for water circulation through chemically resistant Viton tubing to continually wash the inner surface of the test glove via holes in the tubing, a dextrous robot hand operated by a microprocessor, a chemically protective nitrile glove to protect the robot hand, an incubator to maintain 35°C temperature, and a hot plate to maintain 35°C at the sampling point of the circulating water...
October 18, 2016: Journal of Occupational and Environmental Hygiene
Wen Zhou, John Thompson, Clifford Leznoff, Daniel Bernard Leznoff
The preparation and structural characterization of a series of chromium phthalocyanine complexes with multiple metal and ring oxidation states was achieved using PcCr(II) (1) (Pc = phthalocyanine) or PcCr(II)(THF)2 (1·THF2) as starting material. The reaction of soluble 1·THF2 with Br2 or I2 gave the PcCr(III) halide complexes PcCrX(THF) (X= I/I3, Br) (3, 4). Treatment of 1 with one-half equivalent of PhIO or air generated the dinuclear [PcCr(THF)]2(μ-O) (5), while the addition of one equivalent of AgSbF6 to 1 resulted in oxidation to THF-solvated octahedral [PcCr(III)(THF)2]SbF6 (6)...
October 17, 2016: Chemistry: a European Journal
Oon Ha Shin, Dae Yeon Kim, Yong Weon Seo
BACKGROUND: The importance of the effect of phytochemical accumulation in wheat grain on grain physiology has been recognised. In this study, we tracked phytochemical concentration in the seed coat of purple wheat during the water-imbibition phase and also hypothesized that the speed of germination was only relevant to its initial phytochemical concentration. RESULTS: The results indicate that the speed of germination was significantly reduced in the darker grain groups within the purple wheat...
October 18, 2016: Journal of the Science of Food and Agriculture
Marco Sturaro, Enrico Della Gaspera, Niccolò Michieli, Carlo Cantalini, Seyed Mahmoud Emamjomeh, Massimo Guglielmi, Alessandro Martucci
Highly doped wide band gap metal oxides nanocrystals have recently been proposed as building blocks for applications as transparent electrodes, electrochromics, plasmonics, and optoelectronics in general. Here we demonstrate the application of gallium doped zinc oxide (GZO) nanocrystals as novel plasmonic and chemiresistive sensors for the detection of hazardous gases including hydrogen (H2) and nitrogen dioxide (NO2). GZO nanocrystals with a tunable surface plasmon resonance in the near infrared are obtained using a colloidal heat-up synthesis...
October 18, 2016: ACS Applied Materials & Interfaces
Yu-Hong Cheng, Yue Zhang, Siu-Leung Chau, Samuel Kin-Man Lai, Ho-Wai Tang, Kwan-Ming Ng
Metal alloy nanoparticles (NPs) offer a new combination of unique physicochemical properties based on their pure counterparts, which can facilitate the development of novel analytical methods. Here, we demonstrated that Ag-Au alloy NPs could be utilized for optical and mass spectrometric imaging of latent fingerprints (LFPs) with improved image contrast, stability, and detection sensitivity. Upon deposition of Ag-Au alloy NPs (Ag:Au = 60:40% wt), ridge regions of the LFP became amber-coloured, while the groove regions appeared purple-blue...
October 17, 2016: ACS Applied Materials & Interfaces
Cristiane C Denardin, Leo A M Martins, Mariana M Parisi, Moema Queiroz Vieira, Silvia R Terra, Florencia M Barbé-Tuana, Radovan Borojevic, Márcia Vizzotto, Tatiana Emanuelli, Fátima Costa Rodrigues Guma
Activated hepatic stellate cells (HSC) are the major source of collagen I in liver fibrosis. Eugenia uniflora L. is a tree species that is widely distributed in South America. E. uniflora L. fruit-popularly known as pitanga-has been shown to exert beneficial properties. Autophagy contributes to the maintenance of cellular homeostasis and survival under stress situation, but it has also been suggested to be an alternative cell death pathway. Mitochondria play a pivotal role on signaling cell death. Mitophagy of damaged mitochondria is an important cell defense mechanism against organelle-mediated cell death signaling...
October 15, 2016: Cell Biology and Toxicology
B Barman, M Lyngdoh, K G Lynrah, S B Warjri
The purple urine bag syndrome (PUBS) i.e. purple discolouration of urine, is an uncommon and alarming condition in patients with long term urinary catheterisation. Though the condition is benign, it should draw immediate attention to the possibility of an underlying urinary tract infection. The postulated hypothesis for this unusual event is probably a chemical reaction involving tryptophan from food in the gut. We report a case with this unusual and interesting phenomenon in a 65 year old female.
June 2016: Journal of the Association of Physicians of India
Jaime Hernandez-Maldonado, Brendon Stoneburner, Alison Boren, Laurence Miller, Michael Rosen, Ronald S Oremland, Chad W Saltikov
The full genome sequence of Ectothiorhodospira sp. strain BSL-9 is reported here. This purple sulfur bacterium encodes an arxA-type arsenite oxidase within the arxB2AB1CD gene island and is capable of carrying out "photoarsenotrophy" anoxygenic photosynthetic arsenite oxidation. Its genome is composed of 3.5 Mb and has approximately 63% G+C content.
October 13, 2016: Genome Announcements
K Kent, K E Charlton, M Netzel, K Fanning
BACKGROUND: Preclinical evidence suggests that the anthocyanins, which comprise a subclass of dietary flavonoids providing the purple and red pigmentation in plant-based foods, may have a beneficial impact on cognitive outcomes. METHODS: A systematic review was conducted to identify the published literature on food-based anthocyanin consumption and cognitive outcomes in human intervention trials. The literature search followed PRISMA guidelines and included six databases, as well as additional hand searching...
October 11, 2016: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
René Cardeña, Idania Valdez-Vazquez, Germán Buitrón
Purple non-sulfur bacteria generate hydrogen and polyhydroxybutyrate (PHB) as a mechanism for disposing of reducing equivalents generated during substrate consumption. However, both pathways compete for the reducing equivalents released from bacteria growing under certain substrates, thus the formation of hydrogen or PHB is detrimental to the formation of each other. The effect of mixtures of acetic, propionic and butyric acids on the formation of H2 and PHB was evaluated using Box-Behnken design. A bacterial community mainly constituted by Rhodopseudomonas palustris was used as inoculum...
October 11, 2016: Bioprocess and Biosystems Engineering
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