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https://www.readbyqxmd.com/read/28350856/a-fat-derived-metabolite-regulates-a-peptidergic-feeding-circuit-in-drosophila
#1
Do-Hyoung Kim, Minjung Shin, Sung-Hwan Jung, Young-Joon Kim, Walton D Jones
Here, we show that the enzymatic cofactor tetrahydrobiopterin (BH4) inhibits feeding in Drosophila. BH4 biosynthesis requires the sequential action of the conserved enzymes Punch, Purple, and Sepiapterin Reductase (Sptr). Although we observe increased feeding upon loss of Punch and Purple in the adult fat body, loss of Sptr must occur in the brain. We found Sptr expression is required in four adult neurons that express neuropeptide F (NPF), the fly homologue of the vertebrate appetite regulator neuropeptide Y (NPY)...
March 2017: PLoS Biology
https://www.readbyqxmd.com/read/28345675/a-myb-transcription-factor-dcmyb6-is-involved-in-regulating-anthocyanin-biosynthesis-in-purple-carrot-taproots
#2
Zhi-Sheng Xu, Kai Feng, Feng Que, Feng Wang, Ai-Sheng Xiong
Carrots are widely grown and enjoyed around the world. Purple carrots accumulate rich anthocyanins in the taproots, while orange, yellow, and red carrots accumulate rich carotenoids in the taproots. Our previous studies indicated that variation in the activity of regulatory genes may be responsible for variations in anthocyanin production among various carrot cultivars. In this study, an R2R3-type MYB gene, designated as DcMYB6, was isolated from a purple carrot cultivar. In a phylogenetic analysis, DcMYB6 was grouped into an anthocyanin biosynthesis-related MYB clade...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28341328/assessment-of-bioavailability-of-selenium-in-different-plant-soil-systems-by-diffusive-gradients-in-thin-films-dgt
#3
Qin Peng, Mengke Wang, Zewei Cui, Jie Huang, Changer Chen, Lu Guo, Dongli Liang
Uptake of selenium (Se) by plants largely depend on the availability of Se in soil. Soils and plants were sampled four times within 8 weeks of plant growth in pot experiments using four plant species. Sequential extraction and diffusive gradients in thin-films (DGT) method were employed to measure Se concentrations in potted soils in selenite- or selenate-amended soils. Results showed that DGT-measured Se concentrations (CDGT-Se) were generally several folds higher for selenate than selenite amended soils, which were obviously affected by the plant species and the duration of their growth...
March 21, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28340710/selective-colorimetric-analysis-of-spermine-based-on-the-cross-linking-aggregation-of-gold-nanoparticles-chain-assembly
#4
Jian Wang, Zhu Lian Wu, Hong Zhi Zhang, Yuan Fang Li, Cheng Zhi Huang
A selective colorimetric assay for spermine was proposed in this work. In a weak alkaline medium, the conformational structure of double-stranded calf thymus DNA (ctDNA) was loosened to install gold nanoparticles (AuNPs) into chains. While, the chain assembly of AuNPs could form cross-linking aggregates when spermine was present, which was attributed to the electrostatic interaction between the positive change of spermine and negative change both of AuNPs and ctDNA, as well as the groove binding between ctDNA and spermine...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28335325/nanoscale-electric-characteristics-and-oriented-assembly-of-halobacterium-salinarum-membrane-revealed-by-electric-force-microscopy
#5
Denghua Li, Yibing Wang, Huiwen Du, Shiwei Xu, Zhemin Li, Yanlian Yang, Chen Wang
Purple membranes (PM) of the bacteria Halobacterium salinarum are a unique natural membrane where bacteriorhodopsin (BR) can convert photon energy and pump protons. Elucidating the electronic properties of biomembranes is critical for revealing biological mechanisms and developing new devices. We report here the electric properties of PMs studied by using multi-functional electric force microscopy (EFM) at the nanoscale. The topography, surface potential, and dielectric capacity of PMs were imaged and quantitatively measured in parallel...
November 2, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28331912/effect-of-visible-range-electromagnetic-radiations-on-escherichia-coli
#6
Samina T Yousuf Azeemi, Saleem Farooq Shaukat, Khawaja Shamsuddin Azeemi, Idrees Khan, Khalid Mahmood, Farah Naz
BACKGROUND: Escherichia coli is the agent responsible for a range of clinical diseases. With emerging antimicrobial resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range Radiation Therapy/Colour Therapy is an emerging technique in the field of energy/vibrational medicine that uses visible spectrum of Electromagnetic Radiations to cure different diseases. In this study, our goal was to understand the effect of Visible Range Electromagnetic Radiations on E...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28330883/comparative-evaluation-of-sensititre-yeastone%C3%A2-and-clsi-m38-a2-reference-method-for-antifungal-susceptibility-testing-of-aspergillus-spp-against-echinocandins
#7
Maria Siopi, Spyros Pournaras, Joseph Meletiadis
Sensititre YeastOne® (YO) panels were assessed for in vitro susceptibility testing of echinocandins against 39 isolates of A. fumigatus, A. flavus and A. terreus, including two echinocandin-resistant A. fumigatus, using different inocula (10(3), 10(4) and 10(5) cfu/mL), incubation times (16-48h) and endpoints (first blue or purple) and compared with CLSI M38-A2. The best agreement was found with inoculum of 10(4) cfu/mL, incubation time of 20h for A. flavus and 30h for A. fumigatus and A. terreus and reading the first purple well...
March 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28329592/giant-subungual-glomus-tumor-clinical-dermoscopy-imagiologic-and-surgery-details
#8
Ana Filipa Duarte, Osvaldo Correia, Hugo Barreiros, Eckart Haneke
Glomus tumor is a rare benign vascular hamartoma derived from the modified smooth muscle cells of the glomus body. Paroxysmal pain is the leading symptom. A 78-year-old woman presented at our clinic with a 50-year history of a slow growing painful purple blue tender nodule under the right fourth finger nail. Dermoscopy and magnetic ressonance confirmed the presence of a large subungual tumor and total surgical excision was performed after partial nail plate avulsion. Histologic examination confirmed a glomus tumor...
October 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329528/unilateral-striae-distensae-of-the-knee-after-a-steroid-injection-for-the-treatment-of-osgood-schlatter-disease
#9
Kelsey Wise, Donald Warren, Lucia Diaz
We report a 12-year-old girl with new diagnosisof right knee Osgood-Schlatter who developedhorizontal purple striae below the right tibial tubercletwo months after a right knee intra-articular steroidinjection. She is the second reported case of unilaterallocalized striae after an intra-articular steroid injectionand the first with triamcinalone as the corticosteroid.
March 15, 2017: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329517/purple-patches-in-an-immunocompromised-patient-a-report-of-secondary-disseminated-cutaneous-mucormycosis-in-a-man-with-chronic-lymphocytic-leukemia
#10
Sanjana Iyengar, Cindy J Chambers, Jillian W Millsop, Maxwell A Fung, Victoria R Sharon
A 60-year-old man with chronic lymphocytic leukemiadeveloped a deeply violaceous annular patchwith a halo of erythema on the right thigh duringhospitalization for neutropenic fever. Associatedsymptoms included chronic cough and fatigue.Bilateral lung opacities with hilar lymphadenopathywere noted on chest computed tomographyscan. Punch biopsy and tissue culture confirmeda diagnosis of secondary disseminated cutaneousmucormycosis. Although rare, physicians shouldinclude mucormycosis in the differential diagnosisof purpuric patches in immunosuppressed patients...
March 15, 2017: Dermatology Online Journal
https://www.readbyqxmd.com/read/28326068/negative-regulation-of-violacein-biosynthesis-in-chromobacterium-violaceum
#11
Giulia Devescovi, Milan Kojic, Sonia Covaceuszach, Miguel Cámara, Paul Williams, Iris Bertani, Sujatha Subramoni, Vittorio Venturi
In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS, and quorum sensing regulate other phenotypes antagonistically...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28325764/the-vitamin-b12-dependent-photoreceptor-aerr-relieves-photosystem-gene-repression-by-extending-the-interaction-of-crtj-with-photosystem-promoters
#12
Mingxu Fang, Carl E Bauer
Purple nonsulfur bacteria adapt their physiology to a wide variety of environmental conditions often through the control of transcription. One of the main transcription factors involved in controlling expression of the Rhodobacter capsulatus photosystem is CrtJ, which functions as an aerobic repressor of photosystem genes. Recently, we reported that a vitamin B12 binding antirepressor of CrtJ called AerR is required for anaerobic expression of the photosystem. However, the mechanism whereby AerR regulates CrtJ activity is unclear...
March 21, 2017: MBio
https://www.readbyqxmd.com/read/28320948/highly-oriented-photosynthetic-reaction-centers-generate-a-proton-gradient-in-synthetic-protocells
#13
Emiliano Altamura, Francesco Milano, Roberto R Tangorra, Massimo Trotta, Omar Hassan Omar, Pasquale Stano, Fabio Mavelli
Photosynthesis is responsible for the photochemical conversion of light into the chemical energy that fuels the planet Earth. The photochemical core of this process in all photosynthetic organisms is a transmembrane protein called the reaction center. In purple photosynthetic bacteria a simple version of this photoenzyme catalyzes the reduction of a quinone molecule, accompanied by the uptake of two protons from the cytoplasm. This results in the establishment of a proton concentration gradient across the lipid membrane, which can be ultimately harnessed to synthesize ATP...
March 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28318977/aromatic-decoration-determines-the-formation-of-anthocyanic-vacuolar-inclusions
#14
Kalyani Kallam, Ingo Appelhagen, Jie Luo, Nick Albert, Huaibi Zhang, Simon Deroles, Lionel Hill, Kim Findlay, Øyvind M Andersen, Kevin Davies, Cathie Martin
Anthocyanins are some of the most widely occurring secondary metabolites in plants, responsible for the orange, red, purple, and blue colors of flowers and fruits and red colors of autumn leaves. These pigments accumulate in vacuoles, and their color is influenced by chemical decorations, vacuolar pH, the presence of copigments, and metal ions. Anthocyanins are usually soluble in the vacuole, but in some plants, they accumulate as discrete sub-vacuolar structures. Studies have distinguished intensely colored intra-vacuolar bodies observed in the cells of highly colored tissues, termed anthocyanic vacuolar inclusions (AVIs), from more globular, membrane-bound anthocyanoplasts...
March 8, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28318476/p-and-ca-digestibility-is-increased-in-broiler-diets-supplemented-with-the-high-phytase-highphy-wheat
#15
D Scholey, E Burton, N Morgan, C Sanni, C K Madsen, G Dionisio, H Brinch-Pedersen
Around 70% of total seed phosphorus is represented by phytate which must be hydrolysed to be bioavailable in non-ruminant diets. The limited endogenous phytase activity in non-ruminant animals make it common practice to add an exogenous phytase source to most poultry and pig feeds. The mature grain phytase activity (MGPA) of cereal seeds provides a route for the seeds themselves to contribute to phytate digestion, but MGPA varies considerably between species and most varieties in current use make negligible contributions...
March 20, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28317748/bioaccessibility-of-bioactive-compounds-and-antioxidant-potential-of-ju%C3%A3-ara-fruits-euterpe-edulis-martius-subjected-to-in-vitro-gastrointestinal-digestion
#16
Mayara Schulz, Fabíola Carina Biluca, Luciano Valdemiro Gonzaga, Graciele da Silva Campelo Borges, Luciano Vitali, Gustavo Amadeu Micke, Jefferson Santos de Gois, Tarcisio Silva de Almeida, Daniel Lazaro Gallindo Borges, Paul Richard Momsen Miller, Ana Carolina Oliveira Costa, Roseane Fett
An in vitro method involving simulated gastrointestinal digestion was used to assess the bioaccessibility of fifteen minerals, twenty-two phenolic compounds and the antioxidant capacity in juçara fruit during seven ripening stages. For minerals and phenolics, respectively, initial contents were up to 1325.9 and 22.9mg100g(-1), whereas after in vitro digestion, the maximum values were 556.7 and 14.43mg100g(-1) (dry matter). Antioxidant capacity, determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP), after in vitro digestion decreased 51-78% when compared to the crude extract...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28315389/variations-in-the-chemical-composition-and-content-of-salicylic-glycosides-in-the-bark-of-salix-purpurea-from-natural-locations-and-their-significance-for-breeding
#17
Paweł Sulima, Mirosława Krauze-Baranowska, Jerzy Andrzej Przyborowski
Willow bark is one of the few plant raw materials which contain natural active substances that have analgesic, fever-reducing, anti-inflammatory and anti-rheumatic effects. Salix purpurea, listed as widespread and common in many countries, belongs to the willow species with the highest content of salicylic compounds. The chemical composition and content of major salicylic glycosides (SGs) in the bark of S. purpurea genotypes from natural locations were investigated in this study to evaluate their applicability for pharmaceutical processing and creative breeding...
March 15, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28314885/adverse-effects-of-levamisole-in-cocaine-users-a-review-and-risk-assessment
#18
REVIEW
Tibor Markus Brunt, Jorrit van den Berg, Ed Pennings, Bastiaan Venhuis
The immunomodulatory adjuvant and antihelminth levamisole is increasingly used as an adulterant in cocaine worldwide. An accumulating body of clinical and toxicological literature has appeared since 2010 describing neutropenia, agranulocytosis, leukoencephalopathy and vasculitis in cases associated with levamisole-adulterated cocaine. Mostly, neutropenia and agranulocytosis were reported, characterized by a decimation of neutrophils. A large proportion of cases also involved vasculopathy, characterized by pronounced black and purple skin purpura with cutaneous necrosis...
March 17, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28302776/draft-genome-sequences-of-three-closely-related-isolates-of-the-purple-nonsulfur-bacterium-rhodovulum-sulfidophilum
#19
Michael S Guzman, Beau McGinley, Natalia Santiago-Merced, Dinesh Gupta, Arpita Bose
We report here the draft genome sequences of three isolates of Rhodovulum sulfidophilum from a single population that will serve as a model system for understanding genomic traits that underlie metabolic variation within closely related marine purple nonsulfur bacteria in natural microbial communities.
March 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28301580/deep-roots-delay-flowering-and-relax-the-impact-of-floral-traits-and-associated-pollinators-in-steppe-plants
#20
Rachda Berrached, Leila Kadik, Hocine Ait Mouheb, Andreas Prinzing
Strong seasonality in abiotic harshness and pollinator availability shape the reproductive success of plants. Plant species can avoid or can tolerate harsh abiotic conditions and can attract different pollinators, but it remains unknown (i) which of these capacities is most important for flowering phenology, (ii) whether tolerance/avoidance of abiotic harshness reinforces or relaxes the phenological differentiation of species attracting different pollinators. We assembled possibly the first functional trait database for a North African steppe covering 104 species...
2017: PloS One
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