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https://www.readbyqxmd.com/read/28630625/quantification-of-antioxidant-phenolic-compounds-in-a-new-chrysanthemum-cultivar-by-high-performance-liquid-chromatography-with-diode-array-detection-and-electrospray-ionization-mass-spectrometry
#1
Ah-Reum Han, Hyo Young Kim, Yangkang So, Bomi Nam, Ik-Soo Lee, Joo-Won Nam, Yeong Deuk Jo, Sang Hoon Kim, Jin-Baek Kim, Si-Yong Kang, Chang Hyun Jin
The flowers of Chrysanthemum morifolium Ramat. have been used as an herbal tea and in traditional medicine, and the plant has been developed to produce horticultural cultivars of various colors and shapes. In this study, a new chrysanthemum cultivar with dark purple petals (C. morifolium cv. ARTI-Dark Chocolate; ADC) was developed by radiation-induced mutation breeding of its original cultivar with purple striped white petals (C. morifolium cv. Noble Wine, NW). The phenolic profile and antioxidant property of ADC were investigated and compared with NW and the commercially available medicinal herb, C...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28629500/imhoffiella-gen-nov-a-marine-phototrophic-member-of-the-family-chromatiaceae-including-the-description-of-imhoffiella-purpurea-sp-nov-and-the-reclassification-of-thiorhodococcus-bheemlicus-anil-kumar-et-al-2007-as-imhoffiella-bheemlica-comb-nov
#2
Nupur Nupur, Mohit Kumar Saini, Pradeep Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Shinichi Takaichi, Anil Kumar Pinnaka
A coccoid-shaped phototrophic purple sulfur bacterium, strain AK35T, was isolated from a coastal surface water sample collected from Visakhapatnam, India. Cells were Gram-stain-negative, motile and purple, containing bacteriochlorophyll a and the carotenoid rhodopinal as major photosynthetic pigments. Strain AK35T was able to grow photoheterotrophically and could utilize a number of organic substrates. It was unable to grow photoautotrophically. Strain AK35T was able to utilize sulfide and thiosulfate as electron donors...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28627163/molecular-structures-and-functions-of-chlorophylls-a-esterified-with-geranylgeranyl-dihydrogeranylgeranyl-and-tetrahydrogeranylgeranyl-groups-at-the-17-propionate-residue-in-a-diatom-chaetoceros-calcitrans
#3
Tadashi Mizoguchi, Megumi Isaji, Nami Yamano, Jiro Harada, Ritsuko Fujii, Hitoshi Tamiaki
The 17-propionate ester group of chlorophyll(Chl)-a in some oxygenic phototrophs was investigated using HPLC. Chls-a esterified with partially dehydrogenated forms of a phytyl group were found in fully grown cells of a diatom, Chaetoceros calcitrans: geranylgeranyl (GG), dihydrogeranylgeranyl (DHGG), and tetrahydrogeranylgeranyl (THGG). Chls-a bearing such esterifying groups were reported to be found only in greening processes of higher plants, and thus these Chls-a have been thought to be biosynthetic precursors for phytylated Chl-a...
June 19, 2017: Biochemistry
https://www.readbyqxmd.com/read/28626212/a-huge-subcutaneous-hematoma-in-an-adult-with-kasabach-merritt-syndrome
#4
Kuan-Lin Wu, Chiung-Ying Liao, Chen-Kuang Chang, Shang-Yun Ho, Yeu-Sheng Tyan, Yuan-Chun Huang
BACKGROUND Kasabach-Merritt syndrome is a potentially fatal disease that consists of hemangioma(s) with thrombocytopenia, microangiopathic hemolytic anemia, and coagulopathy. Extensive hemangiomatosis is rare. We present the radiological features and treatment strategy of a young adult suffering from Kasabach-Merritt syndrome with widespread hemangiomas and an infected huge hematoma in the right thigh. CASE REPORT A 33-year-old Taiwanese male presented with a painful 20-cm mass over his right thigh and gross hematuria for 2 days...
June 19, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28622347/rna-guided-transcriptional-activation-via-crispr-dcas9-mimics-overexpression-phenotypes-in-arabidopsis
#5
Jong-Jin Park, Emma Dempewolf, Wenzheng Zhang, Zeng-Yu Wang
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) system allows effective gene modification through RNA-guided DNA targeting. The Cas9 has undergone a series of functional alterations from the original active endonuclease to partially or completely deactivated Cas9. The catalytically deactivated Cas9 (dCas9) offers a platform to regulate transcriptional expression with the addition of activator or repressor domains. We redesigned a CRISPR/Cas9 activation system by adding the p65 transactivating subunit of NF-kappa B and a heat-shock factor 1 (HSF) activation domain to dCas9 bound with the VP64 (tetramer of VP16) activation domain for application in plants...
2017: PloS One
https://www.readbyqxmd.com/read/28619130/occurrence-of-mycoplasma-hyorhinis-infections-in-fattening-pigs-and-association-with-clinical-signs-and-pathological-lesions-of-enzootic-pneumonia
#6
Adrian Luehrs, Salome Siegenthaler, Niels Grützner, Elisabeth Grosse Beilage, Peter Kuhnert, Heiko Nathues
Respiratory disorders in fattening pigs are of major concern worldwide. Particularly Enzootic Pneumonia remains a problem for the pig industry. This chronic respiratory disease is primarily caused by Mycoplasma hyopneumoniae (M. hyopneumoniae). However, more recently it was hypothesised that M. hyorhinis can also cause similar lung lesions. To investigate the relevance of M. hyorhinis as a cause of pneumonia in fattening pigs 10 farms in Switzerland (considered free of Enzootic Pneumonia) and 20 farms in Germany (regarded as endemic for Enzootic Pneumonia) with a history of chronic and/or recurrent respiratory diseases were included in the study...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28617283/biosorption-of-six-basic-and-acidic-dyes-on-brown-alga-sargassum-ilicifolium-optimization-kinetic-and-isotherm-studies
#7
Reza Tabaraki, Negar Sadeghinejad
Biosorption of Methyl Blue (MB), Fuchsin Acid (FA), Rhodamine B (RB), Methylene Blue (MEB), Bromocresol purple (BC) and Methyl Orange (MO) onto Sargassum ilicifolium was studied in a batch system. Effect of dye structure on biosorption by Sargassum ilicifolium was studied to define the correlation between chemical structure and biosorption capacity. Different dye groups such as triarylmethane (MB, FA and BC), monoazo (MO), thiazine (MEB) and xanthene (RB) were studied. At optimum experimental conditions for each dye, biosorption capacity was determined and compared...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28613959/reference-intervals-for-the-p-albumin-bromocresol-purple-method
#8
Peter Astrup Christensen
Correct reference intervals are an important part of test results. As establishing own reference intervals is a very expensive task, the NORIP reference intervals are often transferred for use in Nordic laboratories. The NORIP reference interval on P-Albumin was here compared to current results for laboratories using the bromocresol purple (BCP) method for P-Albumin. External quality control reports were used to investigate the change in levels between the BCP and BCG methods on P-Albumin. An algorithm was built for extracting and isolating the laboratory's healthy subject population...
June 14, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28612471/metal-cations-induced-%C3%AE-%C3%AE-bchl-a-heterogeneity-in-lh1-as-revealed-by-temperature-dependent-fluorescence-splitting
#9
Fei Ma, Long-Jiang Yu, Manuel J Llansola-Portoles, Bruno Robert, Zheng-Yu Wang-Otomo, Rienk van Grondelle
Two spectral forms of the core light-harvesting complex (LH1) of the purple bacterium Thermochromatium (Tch.) tepidum, native Ca2+-binding and Ba2+-substituted one, exhibit different fluorescence (FL) emission spectra at low temperature. While Ca-LH1 exhibits one emission band, an unusual splitting of the fluorescence is observed for Ba-LH1. These two sub-bands display the same spectral width dependence according to temperature, but their intensity evolutions with the temperature are different. Based on the crystal structures, we propose that the FL splitting originates from a large αβ-BChl a transition energy heterogeneity, ~600 cm-1, actually much larger than usually in other LH1 and LH2 complexes (80-200 cm-1)...
June 13, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28611239/light-harvesting-in-phototrophic-bacteria-structure-and-function
#10
REVIEW
Rafael G Saer, Robert E Blankenship
This review serves as an introduction to the variety of light-harvesting (LH) structures present in phototrophic prokaryotes. It provides an overview of the LH complexes of purple bacteria, green sulfur bacteria (GSB), acidobacteria, filamentous anoxygenic phototrophs (FAP), and cyanobacteria. Bacteria have adapted their LH systems for efficient operation under a multitude of different habitats and light qualities, performing both oxygenic (oxygen-evolving) and anoxygenic (non-oxygen-evolving) photosynthesis...
June 13, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28610314/two-new-pseudoceros-polycladida-pseudocerotidae-and-a-prostheceraeus-polycladida-euryleptidae-from-andaman-and-nicobar-islands-india
#11
Sudhanshu Dixit, C Raghunathan, Kailash Chandra
Polyclads are free-living marine flatworms known for their striking colour and patterns. These animals though frequently encountered are still understudied in the Indian context. The present paper describes three new species, two belonging to the genus Pseudoceros Lang, 1884 and one to the genus Prostheceraeus Schmarda, 1859. Pseudoceros auranticrinis sp. nov. is characterised by whitish cream background colour, brown mottling with numerous white and dark brown spots all over the body except margins and a smooth dorsal surface with orange pseudotentacles, Pseudoceros vishnui sp...
May 24, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609947/a-new-i-panolis-i-h%C3%A3-bner-1821-species-from-vietnam-lepidoptera-noctuidae-orthosiini
#12
Balázs Benedek, János Babics
Panolis is a well-defined and compact Palearctic trifine Noctuidae genus within the subfamily Hadeninae, tribus Orthosiini. It is currently represented by seven species and one subspecies: Panolis flammea ([Denis & Schiffermüller], 1775), Panolis japonica Draudt, 1935, Panolis variegatoides Poole, 1989, Panolis exquisita Draudt, 1950, Panolis pinicortex Draudt, 1950, Panolis pinicortex exornata Hreblay & Ronkay, 1997, Panolis estheri Ronkay, Ronkay, Gyulai & Hacker, 2010 and Panolis ningshan Wang, Fan, Owada, Wang & Nylin, 2014...
April 20, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609452/effects-of-low-light-on-photosynthetic-properties-antioxidant-enzyme-activity-and-anthocyanin-accumulation-in-purple-pak-choi-brassica-campestris-ssp-chinensis-makino
#13
Hongfang Zhu, Xiaofeng Li, Wen Zhai, Yang Liu, Qianqian Gao, Jinping Liu, Li Ren, Huoying Chen, Yuying Zhu
Anthocyanins are secondary metabolites that contribute to red, blue, and purple colors in plants and are affected by light, but the effects of low light on the physiological responses of purple pak-choi plant leaves are still unclear. In this study, purple pak-choi seedlings were exposed to low light by shading with white gauze and black shading in a phytotron. The responses in terms of photosynthetic properties, carbohydrate metabolism, antioxidant enzyme activity, anthocyanin biosynthetic enzyme activity, and the relative chlorophyll and anthocyanin content of leaves were measured...
2017: PloS One
https://www.readbyqxmd.com/read/28605596/mechanism-of-triplet-energy-transfer-in-photosynthetic-bacterial-reaction-centers
#14
Sarthak Mandal, Anne-Marie Carey, Joshua Locsin, Bing-Rong Gao, JoAnn C Williams, James P Allen, Su Lin, Neal W Woodbury
In purple bacterial reaction centers, triplet excitation energy transfer occurs from the primary donor P, a bacteriochlorophyll dimer, to a neighboring carotenoid to prevent photodamage from the generation of reactive oxygen species. The BB bacteriochlorophyll molecule that lies between P and the carotenoid on the inactive electron transfer branch is involved in triplet energy transfer between P and the carotenoid. To expand the high-resolution spectral and kinetic information available for describing the mechanism, we investigated the triplet excited state formation and energy transfer pathways in the reaction center of Rhodobacter sphaeroides using pump-probe transient absorption spectroscopy over a broad spectral region on the nanosecond to microsecond timescale at both room temperature and at 77 K...
June 12, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28605129/what-is-your-diagnosis-purple-granules-within-neutrophils-of-a-dog
#15
Francisco O Conrado, Rose E Raskin
No abstract text is available yet for this article.
June 12, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28601631/ibt-based-quantitative-proteomics-identifies-potential-regulatory-proteins-involved-in-pigmentation-of-purple-sea-cucumber-apostichopus-japonicus
#16
Lili Xing, Lina Sun, Shilin Liu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are an important economic species and exhibit high yield value among aquaculture animals. Purple sea cucumbers are very rare and beautiful and have stable hereditary patterns. In this study, isobaric tags (IBT) were first used to reveal the molecular mechanism of pigmentation in the body wall of the purple sea cucumber. We analyzed the proteomes of purple sea cucumber in early pigmentation stage (Pa), mid pigmentation stage (Pb) and late pigmentation stage (Pc), resulting in the identification of 5580 proteins, including 1099 differentially expressed proteins in Pb: Pa and 339 differentially expressed proteins in Pc: Pb...
June 2, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28601069/acetate-metabolism-in-the-purple-non-sulfur-bacterium-rhodobacter-capsulatus
#17
E P Petushkova, A A Tsygankov
The purple non-sulfur bacterium Rhodobacter capsulatus B10 can grow on acetate as the sole carbon source under photoheterotrophic conditions. It is known that the bacterium can use the glyoxylate cycle and, in addition to it, or alternatively to it, an unknown pathway for acetate assimilation. We analyzed the genetic potential for functioning of additional metabolic pathways of oxaloacetic acid (OAA) pool replenishment in R. capsulatus. Using published microarray data of more than 4000 transcripts of genes for R...
May 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28600992/a-novel-polydentate-ligand-chromophore-for-simultaneously-colorimetric-detection-of-trace-ag-and-fe3
#18
Zhengquan Yan, Qi Zhao, Meijun Wen, Lei Hu, Xuezhong Zhang, Jinmao You
A novel polydentate ligand chromophore, 3,6-di-(N-ethyl-N-ethoxyl phenylazo) acridine (EEPA), was identified and synthesized. After its structure was characterized by FTIR, (1)H NMR, mass spectra and element analyses, it was noted to find that there was a simultaneously colorimetric response to Ag(+) and Fe(3+) accompanying with different color changes, i.e., from brown to light purple for Ag(+) and further to purple-red for Fe(3+), respectively. Their different action mechanisms, a 1:2 complex mode for EEPA-Ag(+) and 1:1 for EEPA-Fe(3+), were investigated and confirmed by means of Job's plot and theoretical calculation...
June 6, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28598683/buffered-romanowsky-giemsa-method-for-formalin-fixed-paraffin-embedded-sections-taming-a-traditional-stain
#19
D Stefanović, G Samardžija, A Redžek, M Arnaut, Z Nikin, M Stefanović
Romanowsky-Giemsa (RG) stains were devised during the 19th century for identifying plasmodia parasites in blood smears. Later, RG stains became standard procedures for hematology and cytology. Numerous attempts have been made to apply RG staining to formalin-fixed paraffin-embedded tissue sections, with varied success. Most published work on this topic described RG staining methods in which sections were overstained, then subjected to acid differentiation; unfortunately, the differentiation step often caused inconsistent staining outcomes...
June 9, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28592859/synthesis-and-characterization-of-ultralong-sic-nanowires-with-unique-optical-properties-excellent-thermal-stability-and-flexible-nanomechanical-properties
#20
Ping Hu, Shun Dong, Xinghong Zhang, Kaixuan Gui, Guiqing Chen, Ze Hu
Several-millimeter long SiC nanowires (NWs) with unique optical properties, excellent thermal stability and flexible nanomechanical properties were synthesized using a simple method with silicon and phenolic resin as the raw materials. The SiC NWs displayed special optical properties that were attributed to their large size and Al-doping. They displayed broad green emission at 527.8 nm (2.35 eV) and purple emission concentrated at 438.9 nm (2.83 eV), in contrast to the other results, and the synthesized SiC NWs could also remain relatively stable in air up to 1000 °C indicating excellent thermal stability...
June 7, 2017: Scientific Reports
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