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Jin Jeon, Chan Ju Lim, Jae Kwang Kim, Sang Un Park
Pakchoi ( Brassica rapa subsp. chinensis ) is cultivated for its nutritional value, particularly with regard to vitamins, minerals and dietary fibers. However, limited metabolic information is available on the phyto-nutritional traits of pakchoi. Our GC-TOF MS analysis showed that green pakchoi has higher contents of carbon metabolism-associated metabolites such as sugars, sugar derivatives and inositol, while purple pakchoi has higher levels of nitrogen metabolism-associated metabolites such as amino acids and amino acid derivatives...
July 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Diego A Luna-Vital, Elvira Gonzalez de Mejia
The objective of this study was to evaluate the ability of anthocyanins (ANC) present in purple corn to enhance insulin secretion and hepatic glucose uptake in pancreatic cells and hepatocytes, through activation of the free fatty acid receptor-1 (FFAR1) and glucokinase (GK), respectively. Using a dual-layer cell culture with Caco-2 cells, INS-1E or HepG2 cells were treated with an anthocyanin-rich extract from the pericarp of purple corn (PCW), as well as pure ANC cyanidin-3-O-glucoside (C3G), peonidin-3-O-glucoside, pelargonidin-3-O-glucoside...
2018: PloS One
Marcela Cristina Damião Andrucioli, Mírian Aiko Nakane Matsumoto, Maria Conceição Pereira Saraiva, Magda Feres, Luciene Cristina de Figueiredo, Carlos Artério Sorgi, Lucia Helena Faccioli, Raquel Assed Bezerra da Silva, Lea Assed Bezerra da Silva, Paulo Nelson-Filho
OBJECTIVES: Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. MATERIAL AND METHODS: The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment...
July 10, 2018: Journal of Applied Oral Science: Revista FOB
Omar Karray, Rym Batti, Emna Talbi, Haroun Ayed, Marouene Chakroun, Mouna Ayadi Ouarda, Abderrazek Bouzouita, Mohamed Cherif, Mohamed Riadh Ben Slama, Mezlini Amel, Jouida Abdelmoula, Amine Derouiche, Mohamed Chebil
No abstract text is available yet for this article.
September 2018: Urology Case Reports
N Gueneli, A M McKenna, N Ohkouchi, C J Boreham, J Beghin, E J Javaux, J J Brocks
The average cell size of marine phytoplankton is critical for the flow of energy and nutrients from the base of the food web to higher trophic levels. Thus, the evolutionary succession of primary producers through Earth's history is important for our understanding of the radiation of modern protists ∼800 million years ago and the emergence of eumetazoan animals ∼200 million years later. Currently, it is difficult to establish connections between primary production and the proliferation of large and complex organisms because the mid-Proterozoic (∼1,800-800 million years ago) rock record is nearly devoid of recognizable phytoplankton fossils...
July 9, 2018: Proceedings of the National Academy of Sciences of the United States of America
Natta Jaikrajang, Senee Kruanetr, David J Harding, Parawee Rattanakit
The use of natural reagents from plant extracts for chemical analysis is one approach in the development of green analytical chemistry methodology. In this work, a natural reagent extracted from Phyllanthus emblica Linn. has been applied for the determination of iron(III) using a simple flow injection spectrophotometric method. The method was based on the measurement of a dark-purple complex formed by the reaction between iron(III) and the extracted solution in an acetate buffer (pH 5.6) at 570 nm. Under the optimum conditions, a linear calibration graph in the range of 0...
July 3, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Adam Kell, Anton Khmelnitskiy, Mahboobe Jassas, Ryszard J Jankowiak
The LH2 antenna complex of the purple bacterium of Allochromatium vinosum has a distinct double peak structure of the 800 nm band (B800). Several hypotheses were proposed to explain its origin. Recent 77 K two-dimensional electronic spectroscopy data suggested that excitonic coupling of dimerized bacteriochlorophylls (BChls) within the B800 ring is largely responsible for the B800 split [M. Schröter et al., J. Phys. Chem. Lett. 2018, 9, 1340]. Here we argue that the excitonic interactions between BChls in the B800 ring, though present, are weak and cannot explain the B800 band split...
July 9, 2018: Journal of Physical Chemistry Letters
Richard Henderson
Pictures are a key to knowledge: The development of electron microscopy from its beginnings to modern single particle cryo-EM is described by R. Henderson in his Nobel lecture. Shown is the first projection structure at 7 Å resolution of the purple membrane from October 1974.
July 9, 2018: Angewandte Chemie
Stephen W Askew, Gladimir V G Baranoski
Methemoglobinemia and sulfhemoglobinemia are potentially life-threatening blood-related disorders characterized by similar symptoms and markedly distinct treatment procedures. In this paper, we investigate the causal relationship between these conditions and the onset of cyanosis, which is typically associated with a purple or bluish skin coloration. More specifically, we perform controlled experiments to elicit cyanotic appearances resulting from different severity levels of these disorders and varying physiological conditions...
July 1, 2018: Biomedical Optics Express
Agata Kabała-Dzik, Anna Rzepecka-Stojko, Robert Kubina, Marcello Iriti, Robert D Wojtyczka, Ewa Buszman, Jerzy Stojko
Breast cancer is one of the most common causes of mortality in women. Flavonoids, among other compounds, are bioactive constituents of propolis. In this comparative study, we investigated the effects of flavonoids apigenin (API), genistein (GEN), hesperidin (HES), naringin (NAR) and quercetin (QUE) on the proliferation, apoptosis, and cell cycle of two different human cancer cells - MDA-MB-231, estrogen-negative, and MCF-7, estrogen-positive receptor breast carcinoma cells. Many cytotoxic reports of flavonoids were performed by MTT assay...
June 25, 2018: Cellular and Molecular Biology
María de Castro García, Guillermo Antonio Acevedo López
No abstract text is available yet for this article.
July 3, 2018: Medicina Clínica
Bing Guan, Xiao-Hong Li, Liang Wang, Min Zhou, Zhi-Wu Dong, Guo-Jun Luo, Ling-Ping Meng, Jun Hu, Wei-Yun Jin
RATIONALE: Splenosis is the heterotopic auto-transplantation of the splenic tissues. Gastric splenosis in a rare location mimics a gastrointestinal stromal tumor (GIST). Gastric splenosis with hemangioma has not been reported throughout the literature. PATIENT CONCERNS: We report a case of a 74-year-old schistosomiasis cirrhosis splenectomy woman diagnosed with gastric fundus mass. Preoperative computed tomography and endoscopic ultrasonography revealed findings suggestive of a GIST...
July 2018: Medicine (Baltimore)
Magdalena Stârcea, Cristina Gavrilovici, Andra Elsayed, Mihaela Munteanu, Vasile Valeriu Lupu, Elena Cojocaru, Ingrith Miron, Lucian Miron
RATIONALE: Though to be rare, calcific uremic arteriolophathy (CUA) is an ectopic calcification entity causing pain and disabilities in patients with chronic renal insufficiency, thus increasing the morbidity and mortality. PATIENT CONCERN: We report a case of four years old boy admitted with acute respiratory failure. Physical examination revealed: irritability, purple subcutaneous hard nodules, tachypnea, dry spasmodic cough, respiratory rate 45/min, heart rate 110/min, blood pressure 100/60 mmHg, with normal heart sounds, no murmurs, hepatomegaly with hepato-jugular reflux...
July 2018: Medicine (Baltimore)
Hao Zhang, Ling Yang, Wanjian Ding, Yingying Ma
After reporting the mechanisms for purple acid phosphatases against acid environments and alkaline phosphatases against alkaline environments, in the present work, we continued investigating the relationship between catalytic structures of histidine acid phosphatases (HAPs) and acid environments. Based on the comparison of the crystal structures of several HAP members, a series of models were constructed and calculated using density functional theory. Our calculations describe a complete catalytic cycle for HAPs including a free stage and a catalytic reaction stage...
July 5, 2018: Journal of Physical Chemistry. B
Qiang Da, Yuanyuan Gu, Xiafeng Peng, Liying Zhang, Shuhu Du
A colorimetric and visual method is described for the determination of mercury(II) ion. A gel consisting of agar-stabilized silver-coated gold nanoparticles (Au@Ag NPs) was prepared. The reaction with dithiothreitol (DTT) via thiol-Ag chemistry results in an orange to purple color change of the gel. However, in the presence of Hg(II), the reaction of DTT with the silver shells is suppressed due to the strong thiophilicity of Hg(II). The color of the gel changes from purple to red to orange in the presence of increasing concentrations of Hg(II)...
July 4, 2018: Mikrochimica Acta
Qingju Liu, Ping Han, Wenwen Gong, Hui Wang, Xiaoyuan Feng
A method is described for the determination of the pesticide chlorothalonil (CLT). It is based on the finding that citrate-capped gold nanoparticles (AuNPs) undergo aggregation on exposure to chlorothalonil. This is accompanied by a visually detectable color change from wine red to blue. The effect is due to the interaction of the cyano group of chlorothalonil with gold nanoparticles. The assay may also be performed by using a spectrometer. The ratio of absorbances at 700 nm and 520 nm (A700 /A520 ) linearly drops in the 5 to 100 ng·mL-1 CLT concentration range, with a 3...
July 3, 2018: Mikrochimica Acta
Xiaotong Zhou, Lumei Wang, Guoqing Shen, Dongwei Zhang, Jialun Xie, Abdureyim Mamut, Weiwen Huang, Shanshan Zhou
A colorimetric method is presented for the determination of the antibiotic ofloxacin (OFL) in aqueous solution. It is based on the use of an aptamer and gold nanoparticles (AuNPs). In the absence of OFL, the AuNPs are wrapped by the aptamer and maintain dispersed even at the high NaCl concentrations. The solution with colloidally dispersed AuNPs remains red and has an absorption peak at 520 nm. In the presence of OFL, it will bind to the aptamer which is then released from the AuNPs. Hence, AuNPs will aggregate in the salt solution, and color gradually turns to blue, with a new absorption peak at 650 nm...
July 3, 2018: Mikrochimica Acta
Breah LaSarre, David T Kysela, Barry D Stein, Adrien Ducret, Yves V Brun, James B McKinlay
In bacteria and eukaryotes alike, proper cellular physiology relies on robust subcellular organization. For the phototrophic purple nonsulfur bacteria (PNSB), this organization entails the use of a light-harvesting, membrane-bound compartment known as the intracytoplasmic membrane (ICM). Here we show that ICMs are spatially and temporally localized in diverse patterns among PNSB. We visualized ICMs in live cells of 14 PNSB species across nine genera by exploiting the natural autofluorescence of the photosynthetic pigment bacteriochlorophyll (BChl)...
July 3, 2018: MBio
Yonghong Xie
A method is described for the colorimetric determination of mercury(II). In the absence of Hg(II), aminopropyltriethoxysilane (APTES) which is positively charged at pH 7 is electrostatically absorbed on the surface of gold nanoparticles (AuNPs). This neutralizes the negative charges of the AuNPs and leads to NP aggregation and a color change from red to blue-purple. However, in the presence of Hg(II), reduced Hg (formed through the reaction between Hg(II) and citrate on the AuNP surface) will replace the APTES on the AuNPs...
July 2, 2018: Mikrochimica Acta
Amanda Letícia Polli Silvestre, Maria Izabel Milani, Eduardo Luiz Rossini, Leonardo Pezza, Helena Redigolo Pezza
A simple, fast, low-cost, portable, and eco-friendly method using a spot test on a paper platform, together with diffuse reflectance spectroscopy, was developed and validated for the quantification of aluminum hydrochloride, a potential neurotoxic agent, in antiperspirant samples. The determination of aluminum hydrochloride was performed at a wavelength of 615 nm, by measuring consumption of the purple colorimetric reagent Alizarin S, due to reaction with aluminum. The linear range was from 10.0 to 125.0 mg L-1 and could be described by the equation: AR  = 0...
June 15, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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