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https://www.readbyqxmd.com/read/29924858/chemoenzymatic-synthesis-of-sialylated-lactuloses-and-their-inhibitory-effects-on-staphylococcus-aureus
#1
Jie Zeng, Yajie Hu, Tian Jia, Ruiyao Zhang, Tongchao Su, Junliang Sun, Haiyan Gao, Guanglei Li, Meng Cao, Mengdi Song
BACKGROUND: Sialylated glycoconjugates play important roles in physiological and pathological processes. However, available sialylated oligosaccharides source is limited which is a barrier to study their biological roles. This work reports an efficient approach to produce sialic acid-modified lactuloses and investigates their inhibitory effects on Staphylococcus aureus (S. aureus). METHODS: A one-pot two-enzyme (OPTE) sialylation system was used to efficiently synthesize sialylated lactuloses...
2018: PloS One
https://www.readbyqxmd.com/read/29906246/o-glycosylation-mediated-signaling-circuit-drives-metastatic-castration-resistant-prostate-cancer
#2
Sheue-Fen Tzeng, Chin-Hsien Tsai, Tai-Kuang Chao, Yu-Ching Chou, Yu-Chih Yang, Mong-Hsun Tsai, Tai-Lung Cha, Pei-Wen Hsiao
Disseminated castration-resistant prostate cancer (CRPC) is a common disease in men that is characterized by limited survival and resistance to androgen-deprivation therapy. The increase in human epidermal growth factor receptor 2 (HER2) signaling contributes to androgen receptor activity in a subset of patients with CRPC; however, enigmatically, HER2-targeted therapies have demonstrated a lack of efficacy in patients with CRPC. Aberrant glycosylation is a hallmark of cancer and involves key processes that support cancer progression...
June 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29894296/hypoxia-and-serum-deprivation-induces-glycan-alterations-in-triple-negative-breast-cancer-cells
#3
Amanda P B Albuquerque, Meritxell Balmaña, Stefan Mereiter, Filipe Pinto, Celso A Reis, Eduardo I C Beltrão
Triple negative breast cancer (TNBC) is a major global public health problem. The lack of targeted therapy and the elevated mortality evidence the need for better knowledge of the tumor biology. Hypoxia and aberrant glycosylation are associated with advanced stages of malignancy, tumor progression and treatment resistance. Importantly, serum deprivation regulates the invasive phenotype and favors TNBC cell survival. However, in TNBC, the role of hypoxia and serum deprivation in the regulation of glycosylation remains largely unknown...
June 27, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29873256/enzymatic-synthesis-of-novel-quercetin-sialyllactoside-derivatives
#4
Sumangala Darsandhari, Jae Yoon Bae, Biplav Shrestha, Tokutaro Yamaguchi, Hye Jin Jung, Jang Mi Han, Chan-Su Rha, Ramesh Prasad Pandey, Jae Kyung Sohng
Quercetin and its derivatives are important flavonols that show diverse biological activity, such as antioxidant, anticarcinogenic, anti-inflammatory, and antiviral activities. Adding different substituents to quercetin may change the biochemical activity and bioavailability of molecules, when compared to the aglycone. Here, we have synthesised two novel derivatives of quercetin, quercetin-3-O-β-d-glucopyranosyl, 4''-O-d-galactopyranosyl 3'''-O-α-N-acetyl neuraminic acid i.e. 3'-sialyllactosyl quercetin (3'SL-Q) and quercetin-3-O-β-d-glucopyranosyl, 4''-O-β-d-galactopyranosyl 6'''-O-α-N-acetyl neuraminic acid i...
June 6, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29802757/crispr-based-targeted-epigenetic-editing-enables-gene-expression-modulation-of-the-silenced-beta-galactoside-alpha-2-6-sialyltransferase-1-in-cho-cells
#5
Nicolas Marx, Clemens Grünwald-Gruber, Nina Bydlinski, Heena Dhiman, Ly Nguyen Ngoc, Gerald Klanert, Nicole Borth
Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/-out, subclonal variation, induced unknown regulatory effects as well as overexpression stress are still a major hurdle for efficient cell line engineering and for unequivocal characterization of gene function. The use of epigenetic modulators - key players in CHO clonal heterogeneity - has only been marginally addressed so far. Here we present the application of an alternative engineering strategy in CHO cells by utilizing targeted epigenetic editing tools that enable the turning-on or -off of genes without altering the genomic sequence...
May 26, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29765252/triple-negative-and-her2-overexpressing-breast-cancer-cell-sialylation-impacts-tumor-microenvironment-t-lymphocyte-subset-recruitment-a-possible-mechanism-of-tumor-escape
#6
Christian Garbar, Corinne Mascaux, Yacine Merrouche, Armand Bensussan
Introduction: Breast cancers develop different patterns of sialylation to modulate their tumor-infiltrating lymphocyte (TIL) environment. We studied the relationship between α-2,6 sialyltransferases and the TIL in different breast cancer molecular subgroups. Materials and methods: Immunohistochemical preparations were made from 39 luminal (LUM), 13 human epidermal growth factor receptor 2-overexpressing (HER2) and 47 triple-negative (TN) breast carcinomas. Targeted proteins included ST6Gal-I, ST6Gal-II, ST6GalNac-I, CD8, CD4 and granzyme-B in both cytotoxic T lymphocytes and NK lymphocytes (CTL/NK)...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/29749491/expression-analysis-of-st3gal4-transcripts-in-cervical-cancer-cells
#7
Lorena Roa-de La Cruz, Patricia Martínez-Morales, Irene Morán-Cruz, Lorena Milflores-Flores, Nora Rosas-Murrieta, César González-Ramírez, Claudia Ortiz-Mateos, Ricardo Monterrosas-Santamaría, Celestina González-Frías, Carlos Rodea-Ávila, Teresa Apresa-García, Adriana Aguilar-Lemarroy, Luis Jave-Suarez, Gerardo Santos-López, Julio Reyes-Leyva, Verónica Vallejo-Ruiz
ST3GAL4 gene expression is altered in different cancer types, including cervical cancer. Several mRNA transcripts have been reported for this gene; however, their expression levels in cervical cancer have not been analyzed. ST3GAL4 encodes for β‑galactosidase α‑2,3‑sialyltransferase 4, involved in the biosynthesis of the tumour antigens sLe(x) and sulfo‑sLe(x). The present study evaluated the presence of three mRNA variants (V1, V2 and V3) in cervical cancer cell lines, detecting the three variants...
April 27, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29720252/estrogen-induces-st6gal1-expression-and-increases-igg-sialylation-in-mice-and-patients-with-rheumatoid-arthritis-a-potential-explanation-for-the-increased-risk-of-rheumatoid-arthritis-in-postmenopausal-women
#8
Cecilia Engdahl, Albert Bondt, Ulrike Harre, Jasmin Raufer, René Pfeifle, Alessandro Camponeschi, Manfred Wuhrer, Michaela Seeling, Inga-Lill Mårtensson, Falk Nimmerjahn, Gerhard Krönke, Hans U Scherer, Helena Forsblad-d'Elia, Georg Schett
BACKGROUND: Rheumatoid arthritis (RA) preferentially affects women, with the peak incidence coinciding with estrogen decrease in menopause. Estrogen (E2) may therefore have intrinsic immune-regulatory properties that vanish with menopause. Fc sialylation is a crucial factor determining the inflammatory effector function of antibodies. We therefore analyzed whether E2 affects immunoglobulin G (IgG) sialylation. METHODS: Postmenopausal (ovariectomized) mice were immunized with ovalbumin and treated with E2 or vehicle...
May 2, 2018: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29685495/igg-and-leukocytes-targets-of-immunomodulatory-%C3%AE-2-6-sialic-acids
#9
Mark B Jones
ST6Gal1 is a critical sialyltransferase enzyme that controls the addition of α2,6-linked sialic acids to the termini of glycans. Attachment of sialic acids to glycoproteins as a posttranslational modification influences cellular responses, and is a well-known modifier of immune cell behavior. ST6Gal1 activity impacts processes such as: effector functions of immunoglobulin G via Fc sialylation, hematopoietic capacity by hematopoietic stem and progenitor cell surface sialylation, and lymphocyte activation thresholds though CD22 engagement and inhibition of galectins...
March 31, 2018: Cellular Immunology
https://www.readbyqxmd.com/read/29680984/serum-sialylation-changes-in-cancer
#10
REVIEW
Zejian Zhang, Manfred Wuhrer, Stephanie Holst
Cancer is a major cause of death in both developing and developed countries. Early detection and efficient therapy can greatly enhance survival. Aberrant glycosylation has been recognized to be one of the hallmarks of cancer as glycans participate in many cancer-associated events. Cancer-associated glycosylation changes often involve sialic acids which play important roles in cell-cell interaction, recognition and immunological response. This review aims at giving a comprehensive overview of the literature on changes of sialylation in serum of cancer patients...
April 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29680235/transglutaminase-and-sialyltransferase-enzymatic-approaches-for-polymer-conjugation-to-proteins
#11
Katia Maso, Antonella Grigoletto, Gianfranco Pasut
Proteins hold a central role in medicine and biology, also confirmed by the several therapeutic applications based on biologic drugs. Such therapies are of great relevance thanks to high potency and safety of proteins. Nevertheless, many proteins as therapeutics might present issues like fast kidney clearance, rapid enzymatic degradation, or immunogenicity. Such defects implicate frequent administrations or administrations at high doses of the therapeutics, thus yielding or exacerbating potential side effects...
2018: Advances in Protein Chemistry and Structural Biology
https://www.readbyqxmd.com/read/29667779/mir-193a-3p-and-mir-224-mediate-renal-cell-carcinoma-progression-by-targeting-alpha-2-3-sialyltransferase-iv-and-the-phosphatidylinositol-3-kinase-akt-pathway
#12
Yue Pan, Jialei Hu, Jia Ma, Xia Qi, Huimin Zhou, Xiaoyan Miao, Wei Zheng, Li Jia
Tumor metastasis is a major cause of cancer-related death in renal cell carcinoma (RCC). MicroRNAs (miRNAs) have been widely known to modulate proliferation invasion, metastasis, and apoptosis of cancer cells. In this study, we aimed to investigate the function and novel target of miR-193a-3p and miR-224 in RCC. The levels of miR-193a-3p and miR-224 were significantly increased in RCC tissues and RCC cell lines. Alpha-2,3-Sialyltransferase IV (ST3GalIV) was highly expressed in adjacent nontumor tissues and human normal proximal tubular cell line HK-2 compared to RCC tissues and cell lines...
April 18, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29666608/capsular-sialyltransferase-specificity-mediates-different-phenotypes-in-streptococcus-suis-and-group-b-streptococcus
#13
David Roy, Daisuke Takamatsu, Masatoshi Okura, Guillaume Goyette-Desjardins, Marie-Rose Van Calsteren, Audrey Dumesnil, Marcelo Gottschalk, Mariela Segura
The capsular polysaccharide (CPS) represents a key virulence factor for most encapsulated streptococci. Streptococcus suis and Group B Streptococcus (GBS) are both well-encapsulated pathogens of clinical importance in veterinary and/or human medicine and responsible for invasive systemic diseases. S. suis and GBS are the only Gram-positive bacteria which express a sialylated CPS at their surface. An important difference between these two sialylated CPSs is the linkage between the side-chain terminal galactose and sialic acid, being α-2,6 for S...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29624120/density-functional-theory-based-probe-of-the-affinity-interaction-of-saccharide-ligands-with-extra-cellular-sialic-acid-residues
#14
Anjali Patel, Sanjay Tiwari, Prafulla K Jha
Changes in glycosylation pattern leads to malignant transformations among the cells. In combination with upregulated actions of sialyltransferases, it ultimately leads to differential expression of sialic acid (SA) at cell surface. Given its negative charge and localization to extracellular domain, SA has been exploited for the development of targeted theranostics using approaches, such as, cationization and appending recognition saccharides on carrier surface. In this study, we have performed quantum mechanical calculations based on density functional theory (DFT) to study the interaction of saccharides with extracellular SA...
May 10, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29615986/whole-genome-sequencing-and-multiplex-qpcr-methods-to-identify-campylobacter-jejuni-encoding-cst-ii-or-cst-iii-sialyltransferase
#15
Jason M Neal-McKinney, Kun C Liu, Karen C Jinneman, Wen-Hsin Wu, Daniel H Rice
Campylobacter jejuni causes more than 2 million cases of gastroenteritis annually in the United States, and is also linked to the autoimmune sequelae Guillan-Barre syndrome (GBS). GBS often results in flaccid paralysis, as the myelin sheaths of nerve cells are degraded by the adaptive immune response. Certain strains of C. jejuni modify their lipooligosaccharide (LOS) with the addition of neuraminic acid, resulting in LOS moieties that are structurally similar to gangliosides present on nerve cells. This can trigger GBS in a susceptible host, as antibodies generated against C...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29594046/a-cartography-of-siglecs-and-sialyltransferases-in-gynecologic-malignancies-is-there-a-road-towards-a-sweet-future
#16
Quentin Haas, Cedric Simillion, Stephan von Gunten
Altered surface glycosylation is a key feature of cancers, including gynecologic malignancies. Hypersialylation, the overexpression of sialic acid, is known to promote tumor progression and to dampen antitumor responses by mechanisms that also involve sialic acid binding immunoglobulin-like lectins (Siglecs), inhibitory immune receptors. Here, we discuss the expression patterns of Siglecs and sialyltransferases (STs) in gynecologic cancers, including breast, ovarian, and uterine malignancies, based on evidence from The Cancer Genome Atlas...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29569918/sialyltransferase-based-chemoenzymatic-histology-for-the-detection-of-n-and-o-glycans
#17
Aime Lopez Aguilar, Lu Meng, Xiaomeng Hou, Wei Li, Kelley W Moremen, Peng Wu
Profiling specific glycans in histopathological samples is hampered by the lack of selective and sensitive tools for their detection. Here, we report on the development of chemoenzymatic histology of membrane polysaccharide (CHoMP)-based methods for the detection of O- and N-linked glycans on tissue sections via the use of sialyltransferases ST3Gal1 and ST6Gal1, respectively. Combining these two methods, we developed tandem labeling and double labeling strategies that permit the detection of unsialylated and sialylated glycans or the detection of O- and N-linked glycans on the same tissue section, respectively...
April 18, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29543427/%C3%AE-2-6-neosialidase-a-sialyltransferase-mutant-as-a-sialyl-linkage-specific-sialidase
#18
John B McArthur, Hai Yu, Nova Tasnima, Christie M Lee, Andrew J Fisher, Xi Chen
The lack of α2-6-linkage specific sialidases limits the structural and functional studies of sialic-acid-containing molecules. Photobacterium damselae α2-6-sialyltransferase (Pd2,6ST) was shown previously to have α2-6-specific, but weak, sialidase activity. Here, we develop a high-throughput blue-white colony screening method to identify Pd2,6ST mutants with improved α2-6-sialidase activity from mutant libraries generated by sequential saturation mutagenesis. A triple mutant (Pd2,6ST S232L/T356S/W361F) has been identified with 100-fold improved activity, high α2-6-sialyl linkage selectivity, and ability to cleave two common sialic acid forms, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc)...
May 18, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29515098/lncrna-hcp5-promotes-follicular-thyroid-carcinoma-progression-via-mirnas-sponge
#19
Leilei Liang, Jingchao Xu, Meng Wang, Gaoran Xu, Ning Zhang, Guangzhi Wang, Yongfu Zhao
Long non-coding RNAs (lncRNAs), which are important functional regulators in cancer, have received increased attention in recent years. In this study, next-generation sequencing technology was used to identify aberrantly expressed lncRNAs in follicular thyroid carcinoma (FTC). The long non-coding RNA-HLA complex P5 (HCP5) was found to be overexpressed in FTC. The results of the qPCR analysis were consistent with the sequencing results. In addition, functional experiments showed that overexpression of HCP5 can promote the proliferation, migration, invasiveness and angiogenic ability of FTC cells...
March 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29506202/applications-of-a-highly-%C3%AE-2-6-selective-pseudosialidase
#20
Peter Both, Michel Riese, Christopher J Gray, Kun Huang, Edward G Pallister, Iaroslav Kosov, Louis P Conway, Josef Voglmeir, Sabine L Flitsch
Within human biology, combinations of regioisomeric motifs of α2,6- or α2,3-sialic acids linked to galactose are frequently observed attached to glycoconjugates. These include glycoproteins and glycolipids, with each linkage carrying distinct biological information and function. Microbial linkage-specific sialidases have become important tools for studying the role of these sialosides in complex biological settings, as well as being used as biocatalysts for glycoengineering. However, currently, there is no α2,6-specific sialidase available...
February 28, 2018: Glycobiology
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