keyword
MENU ▼
Read by QxMD icon Read
search

sialyltransferase

keyword
https://www.readbyqxmd.com/read/28035776/direct-enzymatic-branch-end-extension-of-glycocluster-presented-glycans-an-effective-strategy-for-programming-glycan-bioactivity
#1
Carlos Bayón, Ning He, Mario Deir-Kaspar, Pilar Blasco, Sabine André, Hans-Joachim Gabius, Ángel Rumbero, Jesús Jiménez-Barbero, Wolf-Dieter Fessner, María J Hernáiz
The sequence of a glycan and its topology of presentation team up to determine the specificity and selectivity of recognition by saccharide receptors (lectins). Structure-activity analysis would be furthered if the glycan part of a glycocluster could be efficiently elaborated in situ while keeping all other parameters constant. By using a bacterial α2,6-sialyltransferase and a small library of bi- to tetravalent glycoclusters, we illustrate the complete conversion of scaffold-presented lactoside units into two different sialylated ligands based on N-acetyl/glycolyl-neuraminic acid incorporation...
November 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28032858/functional-roles-of-sialylation-in-breast-cancer-progression-through-mir-26a-26b-targeting-st8sia4
#2
Xiaolu Ma, Weijie Dong, Zhen Su, Lifen Zhao, Yuan Miao, Nana Li, Huimin Zhou, Li Jia
Sialylation is one of the altered glycosylation patterns associated with cancer progression. In this study, we investigated the N-glycan profiles of breast cancer patients and cell lines to reveal sialylation associated with breast cancer progression, and provided new evidences of miRNA-mediated sialylation. MALDI-TOF MS analysis revealed that N-glycans found in breast cancer tissues and breast cancer cell MDA-MB-231 featured increased levels of sialylation compared with adjacent tissues and normal breast epithelial cell MCF-10A...
December 29, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/28032597/elevation-of-%C3%AE-galactoside-%C3%AE-2-6-sialyltransferase-1-in-a-fructoseresponsive-manner-promotes-pancreatic-cancer-metastasis
#3
Chi-Che Hsieh, Yi-Ming Shyr, Wen-Ying Liao, Tien-Hua Chen, Shin-E Wang, Peir-Chuen Lu, Pei-Yu Lin, Yan-Bo Chen, Wan-Yu Mao, Hsin-Ying Han, Michael Hsiao, Wen-Bin Yang, Wen-Shan Li, Yuh-Pyng Sher, Chia-Ning Shen
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive type of pancreatic cancer with clinical characteristics of local invasion and early metastasis. Recent cohort studies indicate high fructose intake is associated with an increase in pancreatic cancer risk. However, the mechanisms by which fructose promotes pancreatic tumorigenesis remain unclear. Herein, Kras+/LSLG12D mice were crossed with Elas-CreER transgenic mice to determine whether fructose intake directly contributes to tumor formation. Orthotopic tumor-xenograft experiments were performed to determine whether fructose substitution enhances the metastatic potential of PDAC cells...
December 9, 2016: Oncotarget
https://www.readbyqxmd.com/read/28031460/inhibitory-role-of-%C3%AE-2-6-sialylation-in-adipogenesis
#4
Tomoko Kaburagi, Yasuhiko Kizuka, Shinobu Kitazume, Naoyuki Taniguchi
Adipose tissue plays critical roles in obesity and related diseases such as diabetes and cardiovascular diseases. Previous reports suggest that glycans, the most common post-translational modifications, are involved in obesity-related diseases, but what type of glycan regulates adipogenesis during obesity remains unclear. In this study, we first quantified the mRNA levels of 167 genes (encoding 144 glycosyltransferases and 23 related enzymes) in visceral adipose tissues (VATs) from control mice and high fat diet (HFD)-induced obese mice...
December 28, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28002448/unique-molecular-patterns-uncovered-in-kawasaki-disease-patients-with-elevated-serum-gamma-glutamyl-transferase-levels-implications-for-intravenous-immunoglobulin-responsiveness
#5
Yue Wang, Zhen Li, Guang Hu, Shiying Hao, Xiaohong Deng, Min Huang, Miao Ren, Xiyuan Jiang, John T Kanegaye, Kee-Soo Ha, JungHwa Lee, Xiaofeng Li, Xuejun Jiang, Yunxian Yu, Adriana H Tremoulet, Jane C Burns, John C Whitin, Andrew Y Shin, Karl G Sylvester, Doff B McElhinney, Harvey J Cohen, Xuefeng B Ling
BACKGROUND: Resistance to intravenous immunoglobulin (IVIG) occurs in 10-20% of patients with Kawasaki disease (KD). The risk of resistance is about two-fold higher in patients with elevated gamma glutamyl transferase (GGT) levels. We sought to understand the biological mechanisms underlying IVIG resistance in patients with elevated GGT levels. METHOD: We explored the association between elevated GGT levels and IVIG-resistance with a cohort of 686 KD patients (Cohort I)...
2016: PloS One
https://www.readbyqxmd.com/read/27986075/knockdown-of-st6gal-i-increases-cisplatin-sensitivity-in-cervical-cancer-cells
#6
Xiaopeng Zhang, Chunchen Pan, Lei Zhou, Zhaogen Cai, Shufang Zhao, Donghong Yu
BACKGROUND: Sialyltransferase I (ST6Gal-I) is an enzyme involved in tumor metastasis that processes sialic acid precursors into their mature form, enabling them to regulate gene expression. However, the effect of ST6Gal-I on the biological behavior of cancer cells remain unclear. This study was the first to demonstrate the influence of ST6Gal-I on cisplatin sensitivity in cervical cancer cells. METHODS: Knockdown of ST6Gal-I was performed by shRNA and HeLa cells combination with cisplatin were tested...
December 16, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27975143/sialylation-of-n-glycans-mechanism-cellular-compartmentalization-and-function
#7
REVIEW
Gaurang P Bhide, Karen J Colley
Sialylated N-glycans play essential roles in the immune system, pathogen recognition and cancer. This review approaches the sialylation of N-glycans from three perspectives. The first section focuses on the sialyltransferases that add sialic acid to N-glycans. Included in the discussion is a description of these enzymes' glycan acceptors, conserved domain organization and sequences, molecular structure and catalytic mechanism. In addition, we discuss the protein interactions underlying the polysialylation of a select group of adhesion and signaling molecules...
December 14, 2016: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/27943633/integrated-genome-and-protein-editing-swaps-%C3%AE-2-6-sialylation-for-%C3%AE-2-3-sialic-acid-on-recombinant-antibodies-from-cho
#8
Cheng-Yu Chung, Qiong Wang, Shuang Yang, Bojiao Yin, Hui Zhang, Michael Betenbaugh
Immunoglobin G with α-2,6 sialylation has been reported to have an impact on antibody-depend cellular cytotoxicity and anti-inflammatory efficacy. However, production of antibodies with α-2,6 sialylation from Chinese hamster ovary cells is challenging due to the inaccessibility of sialyltransferases for the heavy chain N-glycan site and the presence of exclusively α-2,3 sialyltransferases. In this study, combining mutations on the Fc regions to allow sialyltransferase accessibility with overexpression of α-2,6 sialyltransferase produced IgG with significant levels of both α-2,6 and α-2,3 sialylation...
December 12, 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27871859/alpha2-3-sialyltransferase-iii-knockdown-sensitized-ovarian-cancer-cells-to-cisplatin-induced-apoptosis
#9
Xiaoyu Wang, Yiting Zhang, Haiyingjie Lin, Yan Liu, Yi Tan, Jie Lin, Fenze Gao, Shaoqiang Lin
Emerging evidence indicates that β-galactoside-α2,3-sialyltransferase III (ST3Gal3) involves in development, inflammation, neoplastic transformation, and metastasis. However, the role of ST3Gal3 in regulating cancer chemoresistance remains elusive. Herein, we investigated the functional effects of ST3Gal3 in cisplatin-resistant ovarian cancer cells. We found that the levels of ST3Gal3 mRNA differed significantly among ovarian cancer cell lines. HO8910PM cells that have high invasive and metastatic capacity express elevated ST3Gal3 mRNA and are resistant to cisplatin, comparing to SKOV3 cells that have a lower level of ST3Gal3 expression and are more chemosensitive to cisplatin...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27835877/o-glycan-sialylation-alters-galectin-3-subcellular-localization-and-decreases-chemotherapy-sensitivity-in-gastric-cancer
#10
Sofia N Santos, Mara S Junqueira, Guilherme Francisco, Manuel Vilanova, Ana Magalhães, Marcelo Dias Baruffi, Roger Chammas, Adrian L Harris, Celso A Reis, Emerson S Bernardes
ST6GalNAc-I, the sialyltransferase responsible for sialyl-Tn (sTn) synthesis, has been previously reported to be positively associated with cancer aggressiveness. Here we describe a novel sTn-dependent mechanism for chemotherapeutic resistance. We show that sTn protects cancer cells against chemotherapeutic-induced cell death by decreasing the interaction of cell surface glycan receptors with galectin-3 and increasing its intracellular accumulation. Moreover, exogenously added galectin-3 potentiated the chemotherapeutics-induced cytotoxicity in sTn non-expressing cells, while sTn overexpressing cells were protected...
November 8, 2016: Oncotarget
https://www.readbyqxmd.com/read/27821620/tnf-up-regulates-st3gal4-and-sialyl-lewisx-expression-in-lung-epithelial-cells-through-an-intronic-atf2-responsive-element
#11
Florent Colomb, Marie-Ange Krzewinski-Recchi, Agata Steenackers, Audrey Vincent, Anne Harduin-Lepers, Philippe Delannoy, Sophie Groux-Degroote
We have previously shown that tumor necrosis factor (TNF) induced the up-regulation of the sialyltransferase gene ST3GAL4 (α2,3-sialyltransferase gene) BX transcript through mitogen- and stress-activated kinase 1/2 (MSK1/2), extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) signaling pathways. This up-regulation resulted in sialyl-Lewis(x) (sLe(x)) overexpression on high-molecular-weight glycoproteins in inflamed airway epithelium and increased the adhesion of Pseudomonas aeruginosa PAO1 and PAK strains to lung epithelial cells...
January 1, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/27820809/regulation-of-autoantibody-activity-by-the-il-23-th17-axis-determines-the-onset-of-autoimmune-disease
#12
René Pfeifle, Tobias Rothe, Natacha Ipseiz, Hans U Scherer, Stephan Culemann, Ulrike Harre, Jochen A Ackermann, Martina Seefried, Arnd Kleyer, Stefan Uderhardt, Benjamin Haugg, Axel J Hueber, Patrick Daum, Gordon F Heidkamp, Changrong Ge, Sybille Böhm, Anja Lux, Wolfgang Schuh, Iryna Magorivska, Kutty S Nandakumar, Erik Lönnblom, Christoph Becker, Diana Dudziak, Manfred Wuhrer, Yoann Rombouts, Carolien A Koeleman, René Toes, Thomas H Winkler, Rikard Holmdahl, Martin Herrmann, Stephan Blüml, Falk Nimmerjahn, Georg Schett, Gerhard Krönke
The checkpoints and mechanisms that contribute to autoantibody-driven disease are as yet incompletely understood. Here we identified the axis of interleukin 23 (IL-23) and the TH17 subset of helper T cells as a decisive factor that controlled the intrinsic inflammatory activity of autoantibodies and triggered the clinical onset of autoimmune arthritis. By instructing B cells in an IL-22- and IL-21-dependent manner, TH17 cells regulated the expression of β-galactoside α2,6-sialyltransferase 1 in newly differentiating antibody-producing cells and determined the glycosylation profile and activity of immunoglobulin G (IgG) produced by the plasma cells that subsequently emerged...
January 2017: Nature Immunology
https://www.readbyqxmd.com/read/27807044/golgi-enzymes-do-not-cycle-through-the-endoplasmic-reticulum-during-protein-secretion-or-mitosis
#13
Julien Villeneuve, Juan Duran, Margherita Scarpa, Laia Bassaganyas, Josse Van Galen, Vivek Malhotra
Golgi-specific sialyltransferase (ST) expressed as a chimera with the rapamycin-binding domain of mTOR, FRB, relocates to the endoplasmic reticulum (ER) in cells exposed to rapamycin that also express invariant chain (Ii)-FKBP in the ER. This result has been taken to indicate that Golgi-resident enzymes cycle to the ER constitutively. We show that ST-FRB is trapped in the ER even without Ii-FKBP upon rapamycin addition. This is because ER-Golgi-cycling FKBP proteins contain a C-terminal KDEL-like sequence, bind ST-FRB in the Golgi, and are transported together back to the ER by KDEL receptor-mediated retrograde transport...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27798070/circulating-blood-and-platelets-supply-glycosyltransferases-that-enable-extrinsic-extracellular-glycosylation
#14
Melissa M Lee-Sundlov, David J Ashline, Andrew J Hanneman, Renata Grozovsky, Vernon N Reinhold, Karin M Hoffmeister, Joseph Ty Lau
Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on distal cell surfaces and in the extracellular milieu, the glycan-modifying activities present in systemic circulation have not been systematically examined. Here, we describe an evaluation of blood-borne sialyl-, galactosyl- and fucosyltransferase activities that act upon the four common terminal glycan precursor motifs, GlcNAc monomer, Gal(β3)GlcNAc, Gal(β4)GlcNAc and Gal(β3)GalNAc, to produce more complex glycan structures...
October 26, 2016: Glycobiology
https://www.readbyqxmd.com/read/27787577/alpha-n-acetyl-neuraminide-alpha-2-8-sialyltransferase-1-can-support-immune-responses-toward-tumors-overexpressing-ganglioside-d3-in-mice
#15
Jonathan M Eby, Levi Barse, Steven W Henning, Martijn J W E Rabelink, Jared Klarquist, Emily R Gilbert, Adam M Hammer, Manuel F Fernandez, Nathan Yung, Safia Khan, Hannah G Miller, Edward R Kessler, Elizabeth Garrett-Mayer, Daniel F Dilling, Rob C Hoeben, I Caroline Le Poole
An immunotherapeutic strategy is discussed supporting anti-tumor activity toward malignancies overexpressing ganglioside D3. GD3 can be targeted by NKT cells when derived moieties are presented in the context of CD1d. NKT cells can support anti-tumor responses by secreting inflammatory cytokines and through cytotoxicity toward CD1d(+)GD3(+) tumors. To overexpress GD3, we generated expression vector DNA and an adenoviral vector encoding the enzyme responsible for generating GD3 from its ubiquitous precursor GM3...
October 27, 2016: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/27779707/st3gal-iii-modulates-breast-cancer-cell-adhesion-and-invasion-by-altering-the-expression-of-invasion-related-molecules
#16
Hong-Xia Cui, Honglan Wang, Yuchun Wang, Juan Song, Hua Tian, Chunhui Xia, Yetong Shen
Changes in the carbohydrate structure on the surface of tumor cells is an important feature of cancer metastasis. The specific role of sialic acids in the glycoconjugate terminal has not yet been clearly elucidated in these processes. Previously, we reported that α2,3-sialic acid residues in breast cancer are associated with metastatic potential. The α2,3-sialyltransferase ST3Gal III, which adds α2,3-sialic acids to glycoproteins, is overexpressed in various tumors, and enzyme activity is correlated with tumor metastasis, yet its mechanistic role has not been fully evaluated...
December 2016: Oncology Reports
https://www.readbyqxmd.com/read/27704662/cigarette-smoking-alters-sialylation-in-the-fallopian-tube-of-women-with-implications-for-the-pathogenesis-of-ectopic-pregnancy
#17
Junko Nio-Kobayashi, Hazirah B Z Abidin, Jeremy K Brown, Toshihiko Iwanaga, Andrew W Horne, W Colin Duncan
Sialylation creates a negative charge on the cell surface that can interfere with blastocyst implantation. For example, α2,6-sialylation on terminal galactose, catalyzed by the sialyltransferase ST6GAL1, inhibits the binding of galectin-1, a β-galactoside-binding lectin. We recently reported the potential involvement of galectin-1 and -3 in the pathogenesis of tubal ectopic pregnancy; however, the precise role of galectins and their ligand glycoconjugates remain unclear. Here, we investigated the expression of the genes encoding α2,3- and α2,6-galactoside sialyltransferases (ST3GAL1-6 and ST6GAL1-2) and the localization of sialic acids in the Fallopian tube of women with or without ectopic implantation...
December 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27683310/mice-lacking-sialyltransferase-st3gal-ii-develop-late-onset-obesity-and-insulin-resistance
#18
Pablo Hh Lopez, Susan Aja, Kazuhiro Aoki, Marcus M Seldin, Xia Lei, Gabriele V Ronnett, G William Wong, Ronald L Schnaar
Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the Galβ1-3GalNAc terminus of gangliosides GM1 and GD1b to synthesize GD1a and GT1b, respectively. Mice with a targeted disruption of St3gal2 unexpectedly displayed late-onset obesity and insulin resistance...
September 28, 2016: Glycobiology
https://www.readbyqxmd.com/read/27678392/advancement-of-sialyltransferase-inhibitors-therapeutic-challenges-and-opportunities
#19
Rémi Szabo, Danielle Skropeta
Hypersialylation of tumor cell surface proteins along with a marked upregulation of sialyltransferase (ST) activity is a well-established hallmark of cancer. Due to the critical role of STs in tumor growth and progression, ST inhibition has emerged as a potential new antimetastatic strategy for a range of cancers including pancreatic and ovarian. Human STs are divided into subtypes based on their linkage and acceptor molecule, with each subtype controlling the synthesis of specific sialylated structures with unique biological roles...
September 28, 2016: Medicinal Research Reviews
https://www.readbyqxmd.com/read/27644888/quantum-mechanics-molecular-mechanics-study-of-the-sialyltransferase-reaction-mechanism
#20
Yojiro Hamada, Yusuke Kanematsu, Masanori Tachikawa
The sialyltransferase is an enzyme that transfers the sialic acid moiety from cytidine 5'-monophospho-N-acetyl-neuraminic acid (CMP-NeuAc) to the terminal position of glycans. To elucidate the catalytic mechanism of sialyltransferase, we explored the potential energy surface along the sialic acid transfer reaction coordinates by the hybrid quantum mechanics/molecular mechanics method on the basis of the crystal structure of sialyltransferase CstII. Our calculation demonstrated that CstII employed an SN1-like reaction mechanism via the formation of a short-lived oxocarbenium ion intermediate...
October 11, 2016: Biochemistry
keyword
keyword
25992
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"