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https://www.readbyqxmd.com/read/28822024/glycosaminoglycans-and-glycolipids-as-potential-biomarkers-in-lung-cancer
#1
Guoyun Li, Lingyun Li, Eun Ji Joo, Ji Woong Son, Young Jin Kim, Jae Ku Kang, Kyung Bok Lee, Fuming Zhang, Robert J Linhardt
In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients. The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different...
August 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28795878/effects-of-prisma%C3%A2-skin-dermal-regeneration-device-containing-glycosaminoglycans-on-human-keratinocytes-and-fibroblasts
#2
Raffaella Belvedere, Valentina Bizzarro, Luca Parente, Francesco Petrella, Antonello Petrella
Prisma® Skin is a new pharmaceutical device developed by Mediolanum Farmaceutici S.p.a. It includes alginates, hyaluronic acid and mainly mesoglycan. The latter is a natural glycosaminoglycan preparation containing chondroitin sulfate, dermatan sulfate, heparan sulfate and heparin and it is used in the treatment of vascular disease. Glycosaminoglycans may contribute to the re-epithelialization in the skin wound healing, as components of the extracellular matrix. Here we describe, for the first time, the effects of Prisma® Skin in in vitro cultures of adult epidermal keratinocytes and dermal fibroblasts...
August 10, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28767620/effects-of-training-and-overtraining-on-intervertebral-disc-proteoglycans
#3
Renato H S Ueta, Victor A F Tarini, Carlos E Franciozi, Marcel J S Tamaoki, Valquíria P Medeiros, Helena B Nader, Flávio Faloppa
STUDY DESIGN: Animal experimental study OBJECTIVE.: Evaluate the effect of physical activity and overtraining condition on glycosaminoglycan concentration on the intervertebral disc (IVD) using a rat running model. SUMMARY OF BACKGROUND DATA: Some guidelines recommend the implementation of a physical exercise program as treatment for low back pain, however cyclic loading impact on the health of the intervertebral disc and whether there is a dose-response relationship is still incompletely understood...
August 1, 2017: Spine
https://www.readbyqxmd.com/read/28762851/concentration-of-sulfated-glycosaminoglycans-in-the-mammary-tissue-of-female-rats-with-the-aging-and-about-hormonal-influence
#4
Sueli M P S Torres, Helena B Nader, Ricardo S Simões, Edmund C Baracat, Manuel de J Simões, Luiz F P Fuchs, José Maria Soares-Jr, Regina Célia T Gomes
It was to evaluate the concentration of sulfate glycosaminoglycans (GAG) in mammary tissue of the young and adult female rats and ovariectomized females rats after hormonal stimulation. For this purpose, 60 female rats were divided into six groups with 10 animals/each: nonovariectomized groups: G1 (5 months), and G2 (15 months) and ovariectomized groups: OG (vehicle); EG: (estradiol, 7 days of treatment), PG (progesterone acetate, 23 days of treatment) and EPG: (estradiol (7 days of treatment) and next progesterone acetate (23 days of treatment)...
August 1, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28762182/diet-induced-hypercholesterolemia-alters-liver-glycosaminoglycans-and-associated-lipoprotein-receptors-in-rats
#5
Gangappa Kiran, Ummiti J S Prasada Rao, Paramahans V Salimath, Nandini D Chilkunda
Glycosaminoglycans (GAGs) play an important role in lipoprotein metabolism. In liver, it facilitates the uptake of remnants through receptor-independent endocytosis. However, changes in liver GAGs during diet-induced hypercholesterolemia with normal levels of fat feeding are unknown. Present paper highlights the effect of diet-induced hypercholesterolemia with normal levels (5%) of fat on liver GAGs and other associated lipoprotein receptors. Hypercholesterolemia was induced in rats by feeding diet supplemented with 0...
July 31, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28761999/expression-of-chondroitin-4-o-sulfotransferase-in-escherichia-coli-and-pichia-pastoris
#6
Wenqin He, Yuanyuan Zhu, Abhijeet Shirke, Xiaojun Sun, Jian Liu, Richard A Gross, Mattheos A G Koffas, Robert J Linhardt, Ming Li
Chondroitin sulfates are linear sulfated polysaccharides called glycosaminoglycans. They are important nutraceutical and pharmaceutical products that are biosynthesized through the action of chondroitin sulfotransferases on either an unsulfated chondroitin or a dermatan polysaccharide precursor. While the enzymes involved in the biosynthesis of chondroitin sulfates are well known, the cloning end expression of these membrane-bound Golgi enzymes continue to pose challenges. The major chondroitin-4-sulfotransferase, Homo sapiens C4ST-1, had been previously cloned and expressed from mammalian CHO, COS-7, and HEK 293 cells, and its activity was shown to require glycosylation...
July 31, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28757565/the-pharmaceutical-device-prisma-%C3%A2-skin-promotes-in-vitro-angiogenesis-through-endothelial-to-mesenchymal-transition-during-skin-wound-healing
#7
Raffaella Belvedere, Valentina Bizzarro, Luca Parente, Francesco Petrella, Antonello Petrella
Glycosaminoglycans are polysaccharides of the extracellular matrix supporting skin wound closure. Mesoglycan is a mixture of glycosaminoglycans such as chondroitin-, dermatan-, heparan-sulfate and heparin and is the main component of Prisma(®) Skin, a pharmaceutical device developed by Mediolanum Farmaceutici S.p.a. Here, we show the in vitro effects of this device in the new vessels formation by endothelial cells, since angiogenesis represents a key moment in wound healing. We found a strong increase of migration and invasion rates of these cells treated with mesoglycan and Prisma(®) Skin which mediate the activation of the pathway triggered by CD44 receptor...
July 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28743910/characterization-of-a-propionibacterium-acnes-surface-protein-as-a-fibrinogen-binding-protein
#8
Philippe A Grange, Joël Raingeaud, Willy Morelle, Anne-Geneviève Marcelin, Vincent Calvez, Nicolas Dupin
Propionibacterium acnes (P. acnes) is a major skin-associated bacterium that was long considered commensal, until several studies revealed it to be an opportunistic pathogen. We investigated the ability of P. acnes surface proteins to recognize ECM proteins and showed that a 58 kDa P. acnes surface protein was specifically recognized by human fibrinogen (hFg). The 58 kDa protein was further characterized by two-dimensional (2-D) electrophoresis and MALDI-ToF as a P. acnes host cell-surface attachment protein, PA25957, recognizing dermatan sulfate (DsA1)...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28739506/glycosaminoglycans-from-marine-sources-as-therapeutic-agents
#9
REVIEW
Jesus Valcarcel, Ramón Novoa-Carballal, Ricardo Pérez-Martín, Rui L Reis, José Antonio Vázquez
Glycosaminoglycans (GAGs) in marine animals are different to those of terrestrial organisms, mainly in terms of molecular weight and sulfation. The therapeutic properties of GAGs are related to their ability to interact with proteins, which is very much influenced by sulfation position and patterns. Since currently GAGs cannot be chemically synthesized, they are sourced from natural products, with high intra- but also inter-species variability, in terms of chain length, disaccharide composition and sulfation pattern...
July 21, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28723112/surfen-assembled-graphene-oxide-for-fluorescence-turn-on-detection-of-sulfated-glycosaminoglycans-in-biological-matrix
#10
Yen-Ting Wang, Wei-Lung Tseng
Sulfated glycosaminoglycans (GAGs) not only serve as a biomarker for mucopolysaccharidoses disease but also participate in various biological processes, such as blood clot medication (heparin) and signal transduction (heparan sulfate). However, few fluorescent sensors, such as 1,9-dimethylmethylene blue, have been developed for the detection of sulfated GAGs in the real world. Herein, we fabricated a surfen/few-layer graphene oxide (FLGO) nanocomplex for sensing sulfated GAGs in biological fluids. Surfen molecules are self-assembled onto the surface of FLGO through electrostatic attraction, and their fluorescence was then quenched by the creation of the FLGO-surfen complex (static quenching) and partially combined with the energy transfer from surfen to FLGO (dynamic quenching)...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28721300/extracellular-matrix-alterations-in-the-peyronie-s-disease
#11
Marcelo Silva Watanabe, Thérèse Rachel Theodoro, Natália Lima Coelho, Aline Mendes, Monica Luzia Pereira Leonel, Ana Maria Mader, Helena Bonciani Nader, Sidney Glina, Maria Aparecida Silva Pinhal
Peyronie's disease is characterized by fibrous plaque formation of the tunica albuginea, causing penile deformity and fertility problems. The aim of the present study was to investigate alterations in the extracellular matrix in Peyronie's disease. The study used tissues collected by surgical procedure from individuals that presented a well-established disease, while control samples were obtained by biopsies of fresh cadavers. Immunohistochemistry analysis followed by digital quantification was performed to evaluate TGF-β, heparanases and metalloproteinases (MMPs)...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28698456/combining-nmr-spectroscopy-and-chemometrics-to-monitor-structural-features-of-crude-hep-arin
#12
Lucio Mauri, Maria Marinozzi, Giulia Mazzini, Richard E Kolinski, Michael Karfunkle, David A Keire, Marco Guerrini
Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API). For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions). Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses...
July 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28684085/incomplete-biomarker-response-in-mucopolysaccharidosis-type-i-after-successful-hematopoietic-cell-transplantation
#13
Gé-Ann Kuiper, Peter M van Hasselt, Jaap Jan Boelens, Frits A Wijburg, Eveline J Langereis
BACKGROUND: Residual disease, primarily involving musculoskeletal tissue, is a common problem in patients with neuronopathic mucopolysaccharidosis type I (MPS I, Hurler or severe Hurler-Scheie phenotype) after a successful hematopoietic cell transplantation (HCT). The concentration of the GAG derived biomarkers heparan sulfate (HS) and dermatan sulfate (DS), may reflect residual disease and is used for monitoring biochemical response to therapies. This study investigates the response of HS and DS in blood and urine to HCT in MPS I patients...
May 18, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28678201/characterization-of-danaparoid-complex-extractive-drug-by-an-orthogonal-analytical-approach
#14
Cristina Gardini, Elena Urso, Marco Guerrini, René van Herpen, Pauline de Wit, Annamaria Naggi
Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts. Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones. Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization. Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods...
July 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28676128/phenotype-prediction-for-mucopolysaccharidosis-type-i-by-in-silico-analysis
#15
Li Ou, Michael J Przybilla, Chester B Whitley
BACKGROUND: Mucopolysaccharidosis type I (MPS I) is an autosomal recessive disease due to deficiency of α-L-iduronidase (IDUA), a lysosomal enzyme that degrades glycosaminoglycans (GAG) heparan and dermatan sulfate. To achieve optimal clinical outcomes, early and proper treatment is essential, which requires early diagnosis and phenotype severity prediction. RESULTS: To establish a genotype/phenotype correlation of MPS I disease, a combination of bioinformatics tools including SIFT, PolyPhen, I-Mutant, PROVEAN, PANTHER, SNPs&GO and PHD-SNP are utilized...
July 4, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/28674831/quantification-and-comparison-of-acidic-polysaccharides-in-edible-fish-intestines-and-livers-using-hplc-ms-ms
#16
Shuang Song, Qi Yu, Bao Zhang, Chunqing Ai, Yujiao Sun, Yinghuan Fu, Meiyu Zhao, Chengrong Wen
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry...
July 3, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28651842/sulodexide-prevents-activation-of-the-pla2-cox-2-vegf-inflammatory-pathway-in-human-retinal-endothelial-cells-by-blocking-the-effect-of-age-rage
#17
Giovanni Giurdanella, Francesca Lazzara, Nunzia Caporarello, Gabriella Lupo, Carmelina Daniela Anfuso, Chiara M Eandi, Gian Marco Leggio, Filippo Drago, Claudio Bucolo, Salvatore Salomone
Diabetic retinopathy is characterized by the breakdown of endothelial blood-retinal barrier. We tested the hypothesis that sulodexide (SDX), a highly purified glycosaminoglycan composed of 80% iduronylglycosaminoglycan sulfate and 20% dermatan sulfate, protects human retinal endothelial cells (HREC) from high glucose (HG)-induced damage, through the suppression of inflammatory ERK/cPLA2/COX-2/PGE2 pathway, by blocking the effect of advanced glycation end-products (AGEs). HREC were treated with HG (25mM) or AGEs (glycated-BSA, 2mg/ml) for 48h, with or without SDX (60μg/ml) or aflibercept (AFL, 40μg/ml), a VEGF-trap...
June 23, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28626164/diagnostic-significance-of-measuring-vascular-endothelial-growth-factor-for-the-differentiation-between-malignant-and-tuberculous-pleural-effusion
#18
Hak-Ryul Kim, Byoung-Ryun Kim, Rae-Kil Park, Kwon-Ha Yoon, Eun-Taik Jeong, Ki-Eun Hwang
Malignancy and tuberculosis are common causes of lymphocytic exudative pleural effusion. However, it is occasionally difficult to differentiate malignant pleural effusion from tuberculous pleural effusion. Vascular endothelial growth factor (VEGF) is a critical cytokine in the pathogenesis of malignant pleural effusion. Endocan is a dermatan sulfate proteoglycan that is secreted by endothelial cells. Importantly, endocan mediates the vascular growth-promoting action of VEGF. The aim of this study was to evaluate the diagnostic significance of VEGF and endocan in pleural effusion...
June 2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28622396/mutant-fibulin-3-causes-proteoglycan-accumulation-and-impaired-diffusion-across-bruch-s-membrane
#19
Astrid Zayas-Santiago, Samuel D Cross, James B Stanton, Alan D Marmorstein, Lihua Y Marmorstein
Purpose: The mutation R345W in EFEMP1 (fibulin-3) causes macular degeneration. This study sought to determine whether proteoglycan content and diffusion across Bruch's membrane are altered in Efemp1ki/ki mice carrying this mutation or in Efemp1-/- mice. Methods: Proteoglycans in mouse Bruch's membranes were stained with Cupromeronic Blue (CB). Heparan sulfated proteoglycan (HSPG) and chondroitin/dermatan sulfate proteoglycan (C/DSPG) distributions were visualized following treatments with chondroitinase ABC (C-ABC) or nitrous acid...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28593992/insights-into-hunter-syndrome-from-the-structure-of-iduronate-2-sulfatase
#20
Mykhaylo Demydchuk, Chris H Hill, Aiwu Zhou, Gábor Bunkóczi, Penelope E Stein, Denis Marchesan, Janet E Deane, Randy J Read
Hunter syndrome is a rare but devastating childhood disease caused by mutations in the IDS gene encoding iduronate-2-sulfatase, a crucial enzyme in the lysosomal degradation pathway of dermatan sulfate and heparan sulfate. These complex glycosaminoglycans have important roles in cell adhesion, growth, proliferation and repair, and their degradation and recycling in the lysosome is essential for cellular maintenance. A variety of disease-causing mutations have been identified throughout the IDS gene. However, understanding the molecular basis of the disease has been impaired by the lack of structural data...
June 8, 2017: Nature Communications
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