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Biglycan

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https://www.readbyqxmd.com/read/29129979/hair-germ-model-in-vitro-via-human-postnatal-keratinocyte-dermal-papilla-interactions-impact-of-hyaluronic-acid
#1
Ekaterina Kalabusheva, Vasily Terskikh, Ekaterina Vorotelyak
Hair follicle (HF) reconstruction in vitro is a promising field in alopecia treatment and human HF development research. Here, we combined postnatal human dermal papilla (DP) cells and skin epidermal keratinocytes (KCs) in a hanging drop culture to develop an artificial HF germ. The method is based on DP cell hair-inducing properties and KC self-organization. We evaluated two protocols of aggregate assembling. Mixed HF germ-like structures demonstrated the initiation of epithelial-mesenchymal interaction, including WNT pathway activation and expression of follicular markers...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29129081/identification-of-mature-atherosclerotic-plaque-proteome-signatures-using-data-independent-acquisition-mass-spectrometry
#2
Nicole Hansmeier, Josef Buttigieg, Pankaj Kumar, Shaneen Pelle, Kyoo Yoon Choi, David Kopriva, Tzu-Chiao Chao
Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. In order to gain deeper insights in the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention...
November 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29022912/tenomodulin-is-essential-for-prevention-of-adipocyte-accumulation-and-fibrovascular-scar-formation-during-early-tendon-healing
#3
Dasheng Lin, Paolo Alberton, Manuel Delgado Caceres, Elias Volkmer, Matthias Schieker, Denitsa Docheva
Tenomodulin (Tnmd) is the best-known mature marker for tendon and ligament lineage cells. It is important for tendon maturation, running performance and has key implications for the resident tendon stem/progenitor cells (TSPCs). However, its exact functions during the tendon repair process still remain elusive. Here, we established an Achilles tendon injury model in a Tnmd knockout (Tnmd(-/-)) mouse line. Detailed analyses showed not only a very different scar organization with a clearly reduced cell proliferation and expression of certain tendon-related genes, but also increased cell apoptosis, adipocyte and blood vessel accumulation in the early phase of tendon healing compared with their wild-type (WT) littermates...
October 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28974711/small-leucine-rich-proteoglycans-a-novel-link-to-osteoclastogenesis
#4
Vardit Kram, Tina M Kilts, Nisan Bhattacharyya, Li Li, Marian F Young
Biglycan (Bgn) and Fibromodulin (Fmod) are subtypes of the small leucine-rich family of proteoglycans (SLRP). In this study we examined the skeletal phenotype of BgnFmod double knockout (BgnFmod KO) mice and found they were smaller in size and have markedly reduced bone mass compared to WT. The low bone mass (LBM) phenotype is the result of both the osteoblasts and osteoclasts from BgnFmod KO mice having higher differentiation potential and being more active compared to WT mice. Using multiple approaches, we showed that both Bgn and Fmod directly bind TNFα as well as RANKL in a dose dependent manner and that despite expressing higher levels of both TNFα and RANKL, BgnFmod KO derived osteoblasts cannot retain these cytokines in the vicinity of the cells, which leads to elevated TNFα and RANKL signaling and enhanced osteoclastogenesis...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912302/prevention-of-renal-apob-retention-is-protective-against-diabetic-nephropathy-role-of-tgf-%C3%AE-inhibition
#5
Patricia G Wilson, Joel C Thompson, Meghan H Yoder, Richard Charnigo, Lisa R Tannock
Animal studies demonstrate that hyperlipidemia and renal lipid accumulation contribute to the pathogenesis of diabetic nephropathy. We previously demonstrated that renal lipoproteins co-localize with biglycan, a renal proteoglycan. The purpose of this study was to determine if prevention of renal lipid (apoB) accumulation attenuates diabetic nephropathy. Biglycan deficient and biglycan wildtype Ldlr-/- mice were made diabetic via streptozotocin and fed a high cholesterol diet. As biglycan deficiency is associated with elevated TGF-b, in some experiments mice were injected with either the TGF-b neutralizing antibody 1D11 or with 13C4, an irrelevant control antibody...
September 14, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28883544/global-fibroblast-activation-throughout-the-left-ventricle-but-localized-fibrosis-after-myocardial-infarction
#6
Chandan K Nagaraju, Eef Dries, Natasa Popovic, Abhishek A Singh, Peter Haemers, H Llewelyn Roderick, Piet Claus, Karin R Sipido, Ronald B Driesen
Fibroblast (Fb) differentiation and interstitial fibrosis contribute to cardiac remodeling and loss of function after myocardial infarction (MI). We investigated regional presence and regulation of fibrosis in a pig MI model. In vivo analysis of regional function and perfusion defined three regions: the scar, the myocardium adjacent to the scar (MIadjacent, reduced function, reduced perfusion reserve), and the remote myocardium (MIremote, minimal functional deficit, maintained perfusion). Interstitial and perivascular fibrosis, and increase of collagen type I, was only observed in the MIadjacent...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28882761/decorin-and-biglycan-are-necessary-for-maintaining-collagen-fibril-structure-fiber-realignment-and-mechanical-properties-of-mature-tendons
#7
Kelsey A Robinson, Mei Sun, Carrie E Barnum, Stephanie N Weiss, Julianne Huegel, Snehal S Shetye, Linda Lin, Daniel Saez, Sheila M Adams, Renato V Iozzo, Louis J Soslowsky, David E Birk
The small leucine-rich proteoglycans (SLRPs), decorin and biglycan, are key regulators of collagen fibril and matrix assembly. The goal of this work was to elucidate the roles of decorin and biglycan in tendon homeostasis. Our central hypothesis is that decorin and biglycan expression in the mature tendon would be critical for the maintenance of the structural and mechanical properties of healthy tendons. Defining the function(s) of these SLRPs in tendon homeostasis requires that effects in the mature tendon be isolated from their influence on development...
September 5, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28870349/effect-of-axial-vertical-vibration-on-degeneration-of-lumbar-intervertebral-discs-in-modified-bipedal-rats-an-in-vivo-study
#8
Xiao Liang, Hao Shen, Wei-Dong Shi, Shan Ren, Wei Jiang, Hao Liu, Peng Yang, Zhi-Yong Sun, Jun Lin, Hui-Lin Yang
OBJECTIVE: To assess the effects of axial vibrations on gene expression and lumbar intervertebral disc degeneration in vivo. METHODS: A modified bipedal rat model was established using a brachial plexus rhizotomy approach to imitate human upright posture. The experimental animals were randomly divided into three groups: control, vertical vibration, and whole-body vibration. Gene expression in degeneration of the intervertebral discs was assessed by reverse transcription-quantitative polymerase chain reaction...
July 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28860068/protraction-of-neuropathic-pain-by-morphine-is-mediated-by-spinal-damage-associated-molecular-patterns-damps-in-male-rats
#9
Peter M Grace, Keith A Strand, Erika L Galer, Kenner C Rice, Steven F Maier, Linda R Watkins
We have recently reported that a short course of morphine, starting 10days after sciatic chronic constriction injury (CCI), prolonged the duration of mechanical allodynia for months after morphine ceased. Maintenance of this morphine-induced persistent sensitization was dependent on spinal NOD-like receptor protein 3 (NLRP3) inflammasomes-protein complexes that proteolytically activate interleukin-1β (IL-1β) via caspase-1. However, it is still unclear how NLRP3 inflammasome signaling is maintained long after morphine is cleared...
August 30, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28859141/asporin-deficient-mice-have-tougher-skin-and-altered-skin-glycosaminoglycan-content-and-structure
#10
Marco Maccarana, René B Svensson, Anki Knutsson, Antonis Giannopoulos, Mea Pelkonen, MaryAnn Weis, David Eyre, Matthew Warman, Sebastian Kalamajski
The main structural component of connective tissues is fibrillar, cross-linked collagen whose fibrillogenesis can be modulated by Small Leucine-Rich Proteins/Proteoglycans (SLRPs). Not all SLRPs' effects on collagen and extracellular matrix in vivo have been elucidated; one of the less investigated SLRPs is asporin. Here we describe the successful generation of an Aspn-/- mouse model and the investigation of the Aspn-/- skin phenotype. Functionally, Aspn-/- mice had an increased skin mechanical toughness, although there were no structural changes present on histology or immunohistochemistry...
2017: PloS One
https://www.readbyqxmd.com/read/28834088/vegf-synthesis-is-induced-by-prostacyclin-and-tgf-%C3%AE-in-distal-lung-fibroblasts-from-copd-patients-and-control-subjects-implications-for-pulmonary-vascular-remodelling
#11
Gunilla Westergren-Thorsson, Mariam Bagher, Annika Andersson-Sjöland, Lena Thiman, Claes-Göran Löfdahl, Oskar Hallgren, Leif Bjermer, Anna-Karin Larsson-Callerfelt
BACKGROUND AND OBJECTIVE: Involvement of pulmonary vascular remodelling is a characteristic sign in COPD. Vascular mediators such as vascular endothelial growth factor (VEGF) and prostacyclin may regulate fibroblast activity. The objective was to study the synthesis of VEGF and interactions with prostacyclin and transforming growth factor (TGF)-β1 in lung fibroblasts from patients with COPD and healthy control subjects. To further explore the autocrine role of synthesized VEGF on fibroblast activity, studies were performed in human lung fibroblasts (HFL-1)...
August 22, 2017: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/28830008/up-regulation-of-biglycan-is-associated-with-poor-prognosis-and-pten-deletion-in-patients-with-prostate-cancer
#12
Frank Jacobsen, Juliane Kraft, Cornelia Schroeder, Claudia Hube-Magg, Martina Kluth, Dagmar S Lang, Ronald Simon, Guido Sauter, Jakob R Izbicki, Till S Clauditz, Andreas M Luebke, Andrea Hinsch, Waldemar Wilczak, Corinna Wittmer, Franziska Büscheck, Doris Höflmayer, Sarah Minner, Maria Christina Tsourlakis, Hartwig Huland, Markus Graefen, Lars Budäus, Imke Thederan, Georg Salomon, Thorsten Schlomm, Nathaniel Melling
Biglycan (BGN), a proteoglycan of the extracellular matrix, is included in mRNA signatures for prostate cancer aggressiveness. To understand the impact of BGN on prognosis and its relationship to molecularly defined subsets, we analyzed BGN expression by immunohistochemistry on a tissue microarray containing 12,427 prostate cancers. Seventy-eight percent of 11,050 interpretable cancers showed BGN expression, which was considered as low intensity in 47.7% and as high intensity in 31.1% of cancers. BGN protein expression rose with increasing pathological tumor stage, Gleason grade, lymph node metastasis and early PSA recurrence (P<...
August 19, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28803268/proteomic-profiling-of-the-dystrophin-complex-and-membrane-fraction-from-dystrophic-mdx-muscle-reveals-decreases-in-the-cytolinker-desmoglein-and-increases-in-the-extracellular-matrix-stabilizers-biglycan-and-fibronectin
#13
Sandra Murphy, Heinrich Brinkmeier, Mirjam Krautwald, Michael Henry, Paula Meleady, Kay Ohlendieck
The almost complete loss of the membrane cytoskeletal protein dystrophin and concomitant drastic reduction in dystrophin-associated glycoproteins are the underlying mechanisms of the highly progressive neuromuscular disorder Duchenne muscular dystrophy. In order to identify new potential binding partners of dystrophin or proteins in close proximity to the sarcolemmal dystrophin complex, proteomic profiling of the isolated dystrophin-glycoprotein complex was carried out. Subcellular membrane fractionation and detergent solubilisation, in combination with ion exchange, lectin chromatography and density gradient ultracentrifugation, was performed to isolate a dystrophin complex-enriched fraction...
April 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28800110/response-of-dermal-fibroblasts-to-biochemical-and-physical-cues-in-aligned-polycaprolactone-silk-fibroin-nanofiber-scaffolds-for-application-in-tendon-tissue-engineering
#14
Chih-Hao Chen, Shih-Hsien Chen, Chang-Yi Kuo, Meng-Lun Li, Jyh-Ping Chen
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications. The physicochemical properties of random PCL (RP) nanofibers, random PCL/SF (RPSF) nanofibers and aligned PCL/SF (APSF) nanofibers were characterized for fiber orientation and SF blending effects. An in vitro cell culture with rabbit dermal fibroblasts (RDFBs) on nanofibers indicated that SF promotes cell proliferation to a higher extent than fiber alignment...
August 11, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28789706/small-leucine-rich-proteoglycans-slrps-in-the-endometrium-of-polycystic-ovary-syndrome-women-a-pilot-study
#15
Ricardo Santos Simões, José Maria Soares-Jr, Manuel J Simões, Helena B Nader, Maria Cândida P Baracat, Gustavo Arantes R Maciel, Paulo C Serafini, Ricardo Azziz, Edmund C Baracat
BACKGROUND: Small leucine-rich proteoglycans (SLRPs) play an important role in tissue homeostasis and cell proliferation since these proteoglycans sequester multiple growth factors. However, the content of SLRPs in the endometrium of polycystic ovary syndrome (PCOS) women is unknown. Our purpose was to test the hypothesis that excessive endometrial proliferation in PCOS may be partly related to abnormalities in SLRPs. METHODS: In a cross section study a total of 20 endometrial samples were collected from 10 patients with PCOS and 10 ovulatory women during their proliferative (pre-ovulatory) phase...
August 8, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28763139/tumor-endothelial-cells-accelerate-tumor-metastasis
#16
REVIEW
Nako Maishi, Kyoko Hida
Tumor metastasis is the main cause of cancer-related death. Understanding the molecular mechanisms underlying tumor metastasis is crucial to control this fatal disease. Several molecular pathways orchestrate the complex biological cell events during a metastatic cascade. It is now well known that bidirectional interaction between tumor cells and their microenvironment, including tumor stroma, is important for tumor progression and metastasis. Tumor stromal cells, which acquire their specific characteristics in the tumor microenvironment, accelerate tumor malignancy...
October 2017: Cancer Science
https://www.readbyqxmd.com/read/28759311/biglycan-and-decorin-expression-and-distribution-in-palatal-adhesion
#17
I Ibrahim, M J Serrano, L B Ruest, K K H Svoboda
Previous studies demonstrated that chondroitin sulfate proteoglycans (CSPGs) on apical surfaces of palatal medial edge epithelial (MEE) cells were necessary for palatal adhesion. In this study, we identified 2 proteoglycans, biglycan and decorin, that were expressed in the palatal shelves prior to adhesion. In addition, we established that these proteoglycans were dependent on transforming growth factor β (TGFβ) signaling. Laser capture microdissection was used to collect selected palatal epithelial cells from embryonic mouse embryos at various palate development stages...
November 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28737765/adrenocorticotropic-hormone-and-1-25-dihydroxyvitamin-d3-enhance-human-osteogenesis-in-vitro-by-synergistically-accelerating-the-expression-of-bone-specific-genes
#18
Irina L Tourkova, Li Liu, Nareerat Sutjarit, Quitterie C Larrouture, Jianhua Luo, Lisa J Robinson, Harry C Blair
To improve definition of the physical and hormonal support of bone formation, we studied differentiation of human osteoblasts in vitro at varying combinations of ACTH, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D), and extracellular calcium, with and without added cortisol. Bone mineralization, alkaline phosphatase activity, and osteoblast-specific markers RunX2, osterix, and collagen I increased with 10 pM ACTH, 10 nM 1,25(OH)2D, or at 2 mM calcium with important synergistic activity of combinations of any of these stimuli...
September 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28692113/analysing-the-bioactive-makeup-of-demineralised-dentine-matrix-on-bone-marrow-mesenchymal-stem-cells-for-enhanced-bone-repair
#19
S J Avery, L Sadaghiani, A J Sloan, R J Waddington
Dentine matrix has proposed roles for directing mineralised tissue repair in dentine and bone; however, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA)...
July 10, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28601321/lysophosphatidylcholine-activates-the-akt-pathway-to-upregulate-extracellular-matrix-protein-production-in-human-aortic-valve-cells
#20
Hui Cheng, Qingzhou Yao, Rui Song, Yufeng Zhai, Wei Wang, David A Fullerton, Xianzhong Meng
BACKGROUND: Overproduction of extracellular matrix (ECM) protein by aortic valve interstitial cells (AVICs) plays an important role in valvular sclerosis (thickening) associated with the early pathobiology of aortic stenosis. Accumulation of oxidized low-density lipoprotein (oxLDL) is observed in sclerotic aortic valve and may have a mechanistic role in valvular disease progression. Lysophosphatidylcholine (LysoPC) is a component of oxLDL and has multiple biological activities. This study was to test the hypothesis that oxLDL and LysoPC upregulate ECM protein production in human AVICs...
June 1, 2017: Journal of Surgical Research
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