Seyyed Sajad Daneshi, Lobat Tayebi, Tahereh Talaei-Khozani, Saeid Tavanafar, Amir Hossein Hadaegh, Morteza Rasoulianboroujeni, Banafsheh Rastegari, Seyedeh-Leili Asadi-Yousefabad, Pegah Nammian, Shahrokh Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhelisbayeva, Nader Tanideh, Amin Tamadon
In this study, we propose a spatially patterned 3D-printed nanohydroxyapatite (nHA)/beta-tricalcium phosphate (β-TCP)/collagen composite scaffold incorporating human dental pulp-derived mesenchymal stem cells (hDP-MSCs) for bone regeneration in critical-sized defects. We investigated angiogenesis and osteogenesis in a rabbit critical-sized mandibular defect model treated with this engineered construct. The critical and synergistic role of collagen coating and incorporation of stem cells in the regeneration process was confirmed by including a cell-free uncoated 3D-printed nHA/β-TCP scaffold, a stem cell-loaded 3D-printed nHA/β-TCP scaffold, and a cell-free collagen-coated 3D-printed nHA/β-TCP scaffold in the experimental design, in addition to an empty defect...
April 15, 2024: ACS Biomaterials Science & Engineering