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preadipocyte AND obesity

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https://www.readbyqxmd.com/read/29910623/obesity-associated-mir-27a-upregulation-promotes-hepatocellular-carcinoma-metastasis-through-suppressing-sfrp1
#1
Yang Chen, Fan Zhang, Yawei Zhao, Kan He, Xiao Zheng, Yue Pan, Dan Shao, Pei Shang, Yongsheng Yang, Dan Zhang, Yingjun Xie, Xiaoxiao Yao, Li Chen, Jing Li, Xuewen Zhang
Background: Obesity was a recognized risk factor for the development and progression of hepatocellular carcinoma (HCC). However, the effects and mechanisms by which obesity promotes HCC metastasis remain poorly understood. Materials and methods: We cultured adipocyte induced by preadipocyte 3T3-L1 in vitro and established HCC metastasis model in obesity mouse in vivo to mimic the tumor microenvironment in obese status. The mechanisms underlying obesity-associated miR-27a upregulation promoting HCC metastasis were investigated...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29901265/adipose-tissue-is-a-potential-source-of-hyperandrogenism-in-obese-female-rats
#2
Isabel Viola Wagner, Lena Sahlin, Iuliia Savchuk, Nora Klöting, Konstantin Svechnikov, Olle Söder
OBJECTIVE: Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats. METHODS: Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes...
June 14, 2018: Obesity
https://www.readbyqxmd.com/read/29884798/integrated-analysis-of-long-noncoding-rna-and-mrna-expression-profile-in-children-with-obesity-by-microarray-analysis
#3
Yuesheng Liu, Yuqiang Ji, Min Li, Min Wang, Xiaoqing Yi, Chunyan Yin, Sisi Wang, Meizhen Zhang, Zhao Zhao, Yanfeng Xiao
Long noncoding RNAs (lncRNAs) have an important role in adipose tissue function and energy metabolism homeostasis, and abnormalities may lead to obesity. To investigate whether lncRNAs are involved in childhood obesity, we investigated the differential expression profile of lncRNAs in obese children compared with non-obese children. A total number of 1268 differentially expressed lncRNAs and 1085 differentially expressed mRNAs were identified. Gene Ontology (GO) and pathway analysis revealed that these lncRNAs were involved in varied biological processes, including the inflammatory response, lipid metabolic process, osteoclast differentiation and fatty acid metabolism...
June 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29875667/polyacetylene-from-dendropanax-morbifera-alleviates-diet-induced-obesity-and-hepatic-steatosis-by-activating-ampk-signaling-pathway
#4
Myung-Ji Kang, Eun-Bin Kwon, Hyung Won Ryu, Seoghyun Lee, Jae-Won Lee, Doo-Young Kim, Mi Kyeong Lee, Sei-Ryang Oh, Hyun-Sun Lee, Su Ui Lee, Mun-Ock Kim
The extract tea of Dendropanax morbifera is popular beverages in Korea, and their preventive and therapeutic roles in metabolic disorders have been reported. However, the molecular mechanism has not been studied despite the known efficacy of D. morbifera . Eleven fractions (fr.1-fr.11) were divided by MPLC to find the active compound. Among them, Fr.5 was superior to others in that the inhibitory efficacy of de novo triglyceride (TG) biosynthesis. NMR analysis revealed that Fr.5 is composed 98% or more (9 Z ,16 S )-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (HOD)...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29868672/egcg-stimulates-the-recruitment-of-brite-adipocytes-suppresses-adipogenesis-and-counteracts-tnf-%C3%AE-triggered-insulin-resistance-in-adipocytes
#5
Yashi Mi, Xiao Liu, Haoyu Tian, Hua Liu, Jing Li, Guoyuan Qi, Xuebo Liu
The global rise in obesity and type 2 diabetes has precipitated the need for therapeutic intervention in the arsenal against adiposity. (-)-Epigallocatechin-3-gallate (EGCG), a major nutraceutical component of green tea, has been regarded as a nutraceutical that has powerful antioxidant and anti-obesity bioactivities. In the present study, we showed that EGCG alleviates intracellular lipid accumulation markedly, and the inhibitory effect was largely limited to the early stage of adipocyte differentiation. Consistently, EGCG notably evoked the phosphorylation of AMPK and ACC and blunted the key enzymes of de novo lipogenesis...
June 5, 2018: Food & Function
https://www.readbyqxmd.com/read/29864940/microrna-200b-regulates-preadipocyte-proliferation-and-differentiation-by-targeting-klf4
#6
Linyuan Shen, Mailin Gan, Qiang Li, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
MicroRNAs (miRNAs) are small, non-coding RNAs that affect the post-transcriptional regulation of a variety of biological pathways. A previous study found out that miR-200b was important in the metabolism of obesity, but the mechanism of miR-200b regulateing adipogenesis is not fully understood.. In the present study, we observed that miR200b expression decreased both in obese mice and during the process of 3T3-L1 adipogenic differentiation. Overexpression of miR-200b promoted adipocyte proliferation by regulating the expression of cell cycle-regulated factors...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29795461/epigallocatechin-gallate-targets-fto-and-inhibits-adipogenesis-in-an-mrna-m-6-a-ythdf2-dependent-manner
#7
Ruifan Wu, Yongxi Yao, Qin Jiang, Min Cai, Qing Liu, Yizhen Wang, Xinxia Wang
BACKGROUND/OBJECTIVE: N6 -methyladenosine (m6 A) modification of mRNA plays a role in regulating adipogenesis. However, its underlying mechanism remains largely unknown. Epigallocatechin gallate (EGCG), the most abundant catechin in green tea, plays a critical role in anti-obesity and anti-adipogenesis. METHODS: High-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HPLC-QqQ-MS/MS) was performed to determine the m6 A levels in 3T3-L1 preadipocytes...
May 24, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29777756/peroxiredoxin-5-regulates-adipogenesis-attenuating-oxidative-stress-in-obese-mouse-models-induced-by-a-high-fat-diet
#8
Mi Hye Kim, Sun-Ji Park, Jung-Hak Kim, Jung Bae Seong, Kyung-Min Kim, Hyun Ae Woo, Dong-Seok Lee
Elevated levels of reactive oxygen species (ROS) are a hallmark of obesity. Peroxiredoxin 5 (Prx5), which is a cysteine-dependent peroxidase enzyme, has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Therefore, we focused on the role of Prx5 in regulating mitochondrial ROS and adipogenesis. We demonstrated that Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation...
May 16, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29775748/microrna-204-5p-regulates-3t3-l1-preadipocyte-proliferation-apoptosis-and-differentiation
#9
Jingjing Du, Peiwen Zhang, Mailin Gan, Xue Zhao, Yan Xu, Qiang Li, Yanzhi Jiang, Guoqing Tang, Mingzhou Li, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Obesity due to excessive lipid accumulation is closely associated with metabolic diseases such as type 2 diabetes, insulin resistance and inflammation. Therefore, a detailed understanding of the molecular mechanisms that underlie adipogenesis is crucial to develop treatments for diseases related to obesity. Here, we found that the microRNA-204-5p (miR-204-5p) was expressed at low levels in fat tissues from obese mice fed long-term with a high-fat diet (HFD). Overexpression or inhibition of miR-204-5p in vitro in 3T3-L1 preadipocytes significantly inhibited or promoted 3T3-L1 proliferation, respectively, an effect mediated by regulating cell proliferation factors...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29731242/therapeutic-effects-of-the-allosteric-protein-tyrosine-phosphatase-1b-inhibitor-ky-226-on-experimental-diabetes-and-obesity-via-enhancements-in-insulin-and-leptin-signaling-in-mice
#10
Yuma Ito, Masaki Fukui, Mamoru Kanda, Ko Morishita, Yoshimichi Shoji, Tatsuya Kitao, Eiichi Hinoi, Hiroaki Shirahase
The anti-diabetic and anti-obesity effects of the allosteric protein tyrosine phosphatase 1B (PTP1B) inhibitor 4-(biphenyl-4-ylmethylsulfanylmethyl)-N-(hexane-1-sulfonyl)benzoylamide (KY-226) were pharmacologically evaluated. KY-226 inhibited human PTP1B activity (IC50  = 0.28 μM), but did not exhibit peroxisome proliferator-activated receptor γ (PPARγ) agonist activity. In rodent preadipocytes (3T3-L1), KY-226 up to 10 μM had no effects on adipocyte differentiation, whereas pioglitazone, a PPARγ agonist, markedly promoted it...
March 6, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/29730658/differential-patterns-of-secreted-frizzled-related-protein-4-sfrp4-in-adipocyte-differentiation-adipose-depot-specificity
#11
Hua Guan, Yali Zhang, Shoucui Gao, Liang Bai, Sihai Zhao, Xian Wu Cheng, Jianglin Fan, Enqi Liu
BACKGROUND/AIMS: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that acts as soluble modulators of Wnt signaling. Given the substantial rise in obesity, depot-specific fat accumulation and its associated diseases like diabetes, it is important to understand the molecular basis of depot-specific adipocyte differentiation. In the current study, we investigated the expression of SFRP4 in both subcutaneous and visceral adipose tissue in terms of their differentiation...
April 28, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29722480/3-3-diindolylmethane-enhances-glucose-uptake-through-activation-of-insulin-signaling-in-3t3-l1-adipocytes
#12
Kyeong-Mi Choi, Hwan-Soo Yoo
OBJECTIVE: Indole-3-carbinol (I3C), a naturally occurring compound found in cruciferous vegetables, and its metabolite 3,3'-diindolylmethane (DIM) reduce body mass and serum glucose levels in high-fat-diet-induced obese mice. This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3-L1 adipocytes, as well as the mechanism involved. METHODS: 3T3-L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin...
May 3, 2018: Obesity
https://www.readbyqxmd.com/read/29721149/low-frequency-intermittent-hypoxia-promotes-subcutaneous-adipogenic-differentiation
#13
Yan Wang, Judith C W Mak, Mary Y K Lee, Aimin Xu, Mary S M Ip
Obstructive sleep apnea (OSA), characterized by intermittent hypoxia (IH), is associated with obesity and metabolic disorders. The mass and function of adipose tissue are largely dependent on adipogenesis. The impact of low-frequency IH on adipogenesis is unknown. Sprague-Dawley rats were subjected to IH (4 min for 10% O2 and 2 min for 21% O2 ) or intermittent normoxia (IN) for 6 weeks. The degree of adipogenic differentiation was evaluated by adipogenic transcriptional factors, adipocyte-specific proteins, and oily droplet production in both subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT)...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29720835/combination-of-garcinia-cambogia-extract-and-pear-pomace-extract-additively-suppresses-adipogenesis-and-enhances-lipolysis-in-3t3-l1-cells
#14
Kushal Sharma, Siwon Kang, Dalseong Gong, Sung-Hwa Oh, Eun-Young Park, Min-Ho Oak, Eunyoung Yi
Background: Inhibition of adipogenesis has been a therapeutic target for reducing obesity and obesity-related disorders such as diabetes, hypertension, atherosclerosis, and cancer. For decades, anti-adipogenic potential of many herbal extracts has been investigated. One example is Garcinia cambogia extract (GE) containing (-)-hydroxycitric acid as an active ingredient. GE is currently marketed as a weight loss supplement, used alone or with other ingredients. Pear pomace extract (PE), another natural product, has been also shown to have anti-adipogenic activity in a recent report...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29717274/detecting-epistasis-within-chromatin-regulatory-circuitry-reveals-cand2-as-a-novel-susceptibility-gene-for-obesity
#15
Shan-Shan Dong, Shi Yao, Yi-Xiao Chen, Yan Guo, Yu-Jie Zhang, Hui-Min Niu, Ruo-Han Hao, Hui Shen, Qing Tian, Hong-Wen Deng, Tie-Lin Yang
BACKGROUND: Genome-wide association studies have identified many susceptibility loci for obesity. However, missing heritability problem is still challenging and ignorance of genetic interactions is believed to be an important cause. Current methods for detecting interactions usually do not consider regulatory elements in non-coding regions. Interaction analyses within chromatin regulatory circuitry may identify new susceptibility loci. METHODS: We developed a pipeline named interaction analyses within chromatin regulatory circuitry (IACRC), to identify genetic interactions impacting body mass index (BMI)...
May 1, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29702112/alpha-syntrophin-deficient-mice-are-protected-from-adipocyte-hypertrophy-and-ectopic-triglyceride-deposition-in-obesity
#16
Kristina Eisinger, Lisa Rein-Fischboeck, Markus Neumeier, Sandra Schmidhofer, Rebekka Pohl, Elisabeth M Haberl, Gerhard Liebisch, Andrea Kopp, Andreas Schmid, Sabrina Krautbauer, Christa Buechler
Alpha-syntrophin (SNTA) is a molecular adapter protein which is expressed in adipocytes. Knock-down of SNTA in 3T3-L1 preadipocytes increases cell proliferation, and differentiated adipocytes display small lipid droplets. These effects are both characteristics of healthy adipose tissue growth which is associated with metabolic improvements in obesity. To evaluate a role of SNTA in adipose tissue morphology and obesity associated metabolic dysfunction, SNTA deficient mice were fed a standard chow or a high fat diet...
April 24, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29689639/establishment-and-characterization-of-a-primary-murine-adipose-tissue-chip
#17
Nida Tanataweethum, Adelyn Zelaya, Feipeng Yang, Ronald N Cohen, Eric M Brey, Abhinav Bhushan
Better experimental models are needed to enhance our understanding of metabolic regulation which is seen in obesity and metabolic disorders, such as type 2 diabetes. In vitro models based on microfluidics enable physiological representations of tissues with several advantages over conventional culture systems, such as perfused flow to better mimic the physiological environment. Although cell lines such as 3T3-L1 have been incorporated in microfluidic devices, murine primary preadipocytes have not been differentiated and maintained for long-term monitoring in these culture systems...
April 24, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29687396/isolation-and-differentiation-of-murine-primary-brown-beige-preadipocytes
#18
Hui Yu, Margo Emont, Heejin Jun, Jun Wu
Studies on thermogenic adipose cells (brown and beige fat) hold promise to treat obesity and its associated metabolic disorders due to the ability of these cells to dissipate energy in the form of heat. Although immortalized adipose cell lines have been widely used to investigate cell autonomous regulations, many physiological functions of thermogenic fat need to be studied with primary fat cells. Here, we present a detailed protocol of the isolation, culture, and differentiation of primary brown and beige preadipocytes from the stromal vascular fraction of murine interscapular and inguinal adipose depots...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29663555/dehydroleucodine-inhibits-mitotic-clonal-expansion-during-adipogenesis-through-cell-cycle-arrest
#19
S Abood, M L Veisaga, L A López, M A Barbieri
Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner...
April 16, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29663549/ostreolysin-induces-browning-of-adipocytes-and-ameliorates-hepatic-steatosis
#20
Lili Nimri, Katerina Staikin, Irena Peri, Einav Yehuda-Shnaidman, Betty Schwartz
BACKGROUND AND AIM: Non-alcoholic fatty liver disease (NAFLD) is associated with all features of the metabolic syndrome. Deposition of excess triglycerides in liver cells, a hallmark of NAFLD, is associated with loss of insulin sensitivity. Ostreolysin (Oly) is a 15-kDa fungal protein known to interact with cholesterol-enriched raft-like membrane domains. We aim to test whether a recombinant version of Oly (rOly) can induce functional changes in vitro in adipocytes or in vivo in mice fed a high-fat diet (HFD)...
April 16, 2018: Journal of Gastroenterology and Hepatology
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