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preadipocyte AND obesity

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https://www.readbyqxmd.com/read/28515350/fam3a-enhances-adipogenesis-of-3t3-l1-preadipocytes-via-activation-of-atp-p2-receptor-akt-signaling-pathway
#1
Yujing Chi, Jing Li, Na Li, Zhenzhen Chen, Liping Ma, Weikang Peng, Xiuying Pan, Mei Li, Weidong Yu, Xiangjun He, Bin Geng, Qinghua Cui, Yulan Liu, Jichun Yang
FAM3A plays important roles in regulating hepatic glucose/lipid metabolism and the proliferation of VSMCs. This study determined the role and mechanism of FAM3A in the adipogenesis of 3T3-L1 preadipocytes. During the adipogenesis of 3T3-L1 preadipocytes, FAM3A expression was significantly increased. FAM3A overexpression enhanced 3T3-L1 preadipocyte adipogenesis with increased phosphorylated Akt (pAkt) level, whereas FAM3A silencing inhibited 3T3-L1 preadipocyte adipogenesis with reduced pAkt level. Moreover, FAM3A silencing reduced the expression and secretion of adipokines in 3T3-L1 cells...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28496206/wisp1-ccn4-inhibits-adipocyte-differentiation-through-repression-of-ppar%C3%AE-activity
#2
Nathalie Ferrand, Véronique Béreziat, Marthe Moldes, Maurice Zaoui, Annette K Larsen, Michèle Sabbah
WISP1 (Wnt1-inducible signaling pathway protein-1, also known as CCN4) is a member of the CCN family able to mediate cell growth, transformation and survival in a tissue-specific manner. Here, we report that WISP1 expression was highly increased in preadipocytes and decreased during adipocyte differentiation. Moreover, we observed an increase in WISP1 gene expression in adipose tissue from both diet-induced and leptin-deficient ob/ob obese mice, suggesting that WISP1 could be involved in the pathophysiological onset of obesity...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486516/immortalization-of-chicken-preadipocytes-by-retroviral-transduction-of-chicken-tert-and-tr
#3
Wei Wang, Tianmu Zhang, Chunyan Wu, Shanshan Wang, Yuxiang Wang, Hui Li, Ning Wang
The chicken is an important agricultural animal and model for developmental biology, immunology and virology. Excess fat accumulation continues to be a serious problem for the chicken industry. However, chicken adipogenesis and obesity have not been well investigated, because no chicken preadipocyte cell lines have been generated thus far. Here, we successfully generated two immortalized chicken preadipocyte cell lines through transduction of either chicken telomerase reverse transcriptase (chTERT) alone or in combination with chicken telomerase RNA (chTR)...
2017: PloS One
https://www.readbyqxmd.com/read/28469146/optical-visualisation-of-thermogenesis-in-stimulated-single-cell-brown-adipocytes
#4
Rókus Kriszt, Satoshi Arai, Hideki Itoh, Michelle H Lee, Anna G Goralczyk, Xiu Min Ang, Aaron M Cypess, Andrew P White, Farnaz Shamsi, Ruidan Xue, Jung Yeol Lee, Sung-Chan Lee, Yanyan Hou, Tetsuya Kitaguchi, Thankiah Sudhaharan, Shin'ichi Ishiwata, E Birgitte Lane, Young-Tae Chang, Yu-Hua Tseng, Madoka Suzuki, Michael Raghunath
The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28466023/adipose-tissue-specialized-immunologic-features-might-be-the-potential-therapeutic-target-of-prospective-medicines-for-obesity
#5
REVIEW
Fan Yao, Ming Zhang, Li Chen
Excessive lipid accumulation in adipose tissue is either the source of obesity or the cause and result of chronic local inflammation, and recent studies indicate that the accumulation may induce many other specialized immunologic features with macrophages and epidemic diseases. We analyze the effective stages of immune cells in adipose tissue, including macrophage recruitment, macrophage polarization, and macrophage-like phenotype preadipocyte possession to find optimal sites as drug targets. Subsequently, some main signaling pathways are summarized in this review, including the AMP-activated protein kinase (AMPK) pathway, the JNK signaling pathway, and a novel one, the Notch signaling pathway...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28465089/adipocyte-lipid-storage-and-adipokine-production-are-modulated-by-lipoxygenase-derived-oxylipins-generated-from-18-carbon-fatty-acids
#6
Peter Zahradka, Shannon Neumann, Harold M Aukema, Carla G Taylor
Generation of oxylipins (oxygenated metabolites of fatty acids) by lipoxygenases may be responsible for the beneficial effects of 20- and 22-carbon n-3 fatty acids on adipose tissue dysfunction in obesity, but the potential actions of oxylipins derived from 18-carbon fatty acids, which are generally at higher levels in the diet, are unknown. We therefore compared the effects of select lipoxygenase-derived oxylipins produced from α-linolenic acid (ALA, C18:3 n-3), linoleic acid (LA, C18:2 n-6), and arachidonic acid (AA, C20:4 n-6) on key adipocyte functions that are altered in obesity...
April 29, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28464422/recombinant-ostreolysin-induces-brown-fat-like-phenotype-in-hib-1b-cells
#7
Tom Oren, Lili Nimri, Einav Yehuda-Shnaidman, Katy Staikin, Yitzhak Hadar, Assaf Friedler, Hadar Amartely, Michal Slutzki, Antonella Di Pizio, Masha Y Niv, Irena Peri, Lutz Graeve, Betty Schwartz
SCOPE: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes...
May 2, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28463991/selective-effect-of-phosphatidylcholine-on-the-lysis-of-adipocytes
#8
Ji-Young Kim, Min-Seo Kwon, Junghyun Son, Sang-Wook Kang, Youngsup Song
Obesity, a serious health risk factor, is often associated with depression and negatively affects many aspects of life. Injection of a formula comprising phosphatidylcholine (PPC) and deoxycholate (DC) has emerged as an alternative to liposuction in the reduction of local fat deposits. However, the formula component mainly responsible for this effect and the mechanism behind the actions of the components with respect to fat reduction are unknown. Here, we investigate the specific effects of PPC and DC on adipocyte viability...
2017: PloS One
https://www.readbyqxmd.com/read/28449555/capsanthin-inhibits-both-adipogenesis-in-3t3-l1-preadipocytes-and-weight-gain-in-high-fat-diet-induced-obese-mice
#9
Sung Jun Jo, Jeung Won Kim, Hye Ok Choi, Jung Hwan Kim, Hyung Joong Kim, Sun Hee Woo, Byung Hoon Han
Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis (IC₅₀; 2.5 μM) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes (ED₅₀; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-β₂-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss...
May 1, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28447033/prokineticin-is-a-new-linker-between-obesity-and-cardiovascular-diseases
#10
REVIEW
Canan G Nebigil
Obesity is a fast growing epidemic event worldwide. Fatness is associated with a number of comorbidities, including cardiovascular diseases (CVDs). Although obesity can be heredity in 30-70% cases, the environmental contributions also play an important role in the increasing prevalence of obesity. The relationship between development of obesity and CVD is poorly characterized. Obesity and CVD can also be resulted from a common mechanism such as metabolic, inflammatory, and neurohormonal changes. Prokineticins are defined as cytokines (immunoregulatory proteins), adipokines (adipocyte-secreted hormone), angiogenic (increasing vessel formation), or aneroxic (lowering food intake) hormones...
2017: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28445821/the-ampk-mtor-pathway-is-involved-in-d-dopachrome-tautomerase-gene-transcription-in-adipocytes-differentiated-from-sgbs-cells-a-human-preadipocyte-cell-line
#11
Takeo Iwata, Kyoko Kuribayashi, Masahiko Nakasono, Noriko Saito-Tarashima, Noriaki Minakawa, Noriko Mizusawa, Rie Kido, Katsuhiko Yoshimoto
In adipose tissue, D-dopachrome tautomerase (DDT), a cytokine with structural similarity to macrophage migration inhibitory factor, is mainly expressed in adipocytes rather than preadipocytes and acts as an anti-obesity adipokine in an autocrine manner. However, its transcriptional regulation is largely unknown. In order to explore molecules affecting DDT transcription, a chemical library screening using HEK293 cells stably expressing a DDT promoter-reporter construct was performed. Several derivatives of 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, were identified as transcriptional activators of the DDT gene...
April 23, 2017: Cytokine
https://www.readbyqxmd.com/read/28445487/bmt-decreases-hfd-induced-weight-gain-associated-with-decreased-preadipocyte-number-and-insulin-secretion
#12
Saeed Katiraei, Lisa R Hoving, Lianne van Beek, Sharida Mohamedhoesein, Françoise Carlotti, Janna A van Diepen, Patrick C N Rensen, Mihai G Netea, Ko Willems van Dijk, Jimmy F P Berbée, Vanessa van Harmelen
Experimental bone marrow transplantation (BMT) in mice is commonly used to assess the role of immune cell-specific genes in various pathophysiological settings. The application of BMT in obesity research is hampered by the significant reduction in high-fat diet (HFD)-induced obesity. We set out to characterize metabolic tissues that may be affected by the BMT procedure and impair the HFD-induced response. Male C57BL/6 mice underwent syngeneic BMT using lethal irradiation. After a recovery period of 8 weeks they were fed a low-fat diet (LFD) or HFD for 16 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28426713/metallothioneins-regulate-the-adipogenic-differentiation-of-3t3-l1-cells-via-the-insulin-signaling-pathway
#13
Yoshito Kadota, Yuriko Toriuchi, Yuka Aki, Yuto Mizuno, Takashige Kawakami, Tomoko Nakaya, Masao Sato, Shinya Suzuki
Knockout of metallothionein (MT) genes contributes to a heavier body weight in early life and the potential to become obese through the intake of a high fat diet (HFD) in mice. It has thus been suggested that MT genes regulate the formation of adipose tissue, which would become the base for later HFD-induced obesity. We evaluated the fat pads of mice during the lactation stage. The fat mass and adipocyte size of MT1 and MT2 knockout mice were greater than those of wild type mice. Next, we assayed the ability of small interfering RNA (siRNA) to silence MT genes in the 3T3-L1 cell line...
2017: PloS One
https://www.readbyqxmd.com/read/28417922/indole-derivatives-isolated-from-brown-alga-sargassum-thunbergii-inhibit-adipogenesis-through-ampk-activation-in-3t3-l1-preadipocytes
#14
Min-Cheol Kang, Yuling Ding, Eun-A Kim, Youn Kyung Choi, Thiago de Araujo, Soo-Jin Heo, Seung-Hong Lee
Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives (STCs)-indole-2-carboxaldehyde (STC-1), indole-3-carboxaldehyde (STC-2), indole-4-carboxaldehyde (STC-3), indole-5-carboxaldehyde (STC-4), indole-6-carboxaldehyde (STC-5), and indole-7-carboxaldehyde (STC-6)-from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells...
April 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28414075/vitamin-b2-deficiency-enhances-the-pro-inflammatory-activity-of-adipocyte-consequences-for-insulin-resistance-and-metabolic-syndrome-development
#15
Agnieszka Irena Mazur-Bialy, Ewa Pocheć
AIMS: Adipose tissue is an endocrine organ important for regulation of such physiological processes as energy metabolism or lipids homeostasis. In an obesity state, it participates in the induction of chronic systemic inflammation accompanied by pro-inflammatory cytokines and fatty acid elevation. For this reasons, adipose tissue is involved in, e.g., insulin resistance, type 2 diabetes or hyperlipidemia development. In our previous study, we have shown that riboflavin deficiency induces a pathological pro-inflammatory response of macrophages, the main component of adipose tissue...
June 1, 2017: Life Sciences
https://www.readbyqxmd.com/read/28410190/dietary-oleuropein-inhibits-tumor-angiogenesis-and-lymphangiogenesis-in-the-b16f10-melanoma-allograft-model-a-mechanism-for-the-suppression-of-high-fat-diet-induced-solid-tumor-growth-and-lymph-node-metastasis
#16
Hyerim Song, Do Young Lim, Jae In Jung, Han Jin Cho, So Young Park, Gyoo Taik Kwon, Young-Hee Kang, Ki Won Lee, Myung-Sook Choi, Jung Han Yoon Park
Previously, we reported that high-fat-diet (HFD)-induced obesity stimulates melanoma progression in the B16F10 allograft model. In this study, we examined whether oleuropein (OL), the most abundant phenolic compound in olives, inhibits HFD-induced melanoma progression. Four-week-old male C57BL/6N mice were fed a HFD-diet with or without OL. After 16 weeks of feeding, B16F10-luc cells were subcutaneously injected and the primary tumor was resected 3 weeks later. OL suppressed HFD-induced solid tumor growth. In the tumor tissues, OL reduced HFD-induced expression of angiogenesis (CD31, VE-cadherin, VEGF-A, and VEGFR2), lymphangiogenesis (LYVE-1, VEGF-C, VEGF-D, and VEGFR3), and hypoxia (HIF-1α and GLUT-1) markers as well as HFD-induced increases in lipid vacuoles and M2 macrophages (MΦs)...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28398659/hypaphorine-an-indole-alkaloid-isolated-from-caragana-korshinskii-kom-inhibites-3t3-l1-adipocyte-differentiation-and-improves-insulin-sensitivity-in-vitro
#17
Guangxiang Luan, Fangfang Tie, Zhenzhen Yuan, Gang Li, Jie He, Zhenhua Wang, Honglun Wang
Obesity, a major health problem worldwide, is a complex multifactorial chronic disease that increases the risk for insulin resistance, type 2 diabetes, coronary heart disease and hypertension. In this study, we assessed methods to isolate hypaphorine, a potent drug candidate for obesity and insulin resistance. Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) was established as a method to separate three compounds, adenosine, L-tryptophan and hypaphorine, from the crude extracts of Caragana korshinskii Kom...
April 11, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28393240/do-in-seung-gi%C3%A2-tang-extract-suppresses-adipocyte-differentiation-in-3t3%C3%A2-l1-cells
#18
Eun Hyoung Shim, Myeong Soo Lee, Ju-Ah Lee, Hoyoung Lee
Blood stasis syndrome (BSS), additionally called Eohyul, is a basic pathological concept in Traditional Korean Medicine. Do In Seung Gi‑Tang (DISGT) is herbal medicine used for the treatment of BSS. It primarily treats metabolic diseases (MDs) including obesity, hypertension, diabetes mellitus and gynecological diseases, by promoting blood circulation. The present study aimed to investigate the anti‑adipogenesis effect of DISGT in 3T3‑L1 adipocytes using Oil Red O staining, and assessing levels of triglycerides (TGs) and leptin in adipocytes by ELISA and western blot analysis...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28391908/apob-lipoproteins-and-dysfunctional-white-adipose-tissue-relation-to-risk-factors-for-type-2-diabetes-in-humans
#19
Valérie Lamantia, Simon Bissonnette, Hanny Wassef, Yannick Cyr, Alexis Baass, Robert Dufour, Rémi Rabasa-Lhoret, May Faraj
BACKGROUND: Elevated plasma apoB is an independent predictor of T2D; however, underlying mechanisms remain unclear. Chronic reduction in white adipose tissue (WAT) function promotes T2D. We reported that differentiation of preadipocytes and acute incubation of human WAT with LDL induce their dysfunction (decreased hydrolysis and storage of triglyceride-rich lipoproteins [TRL]). OBJECTIVE: To examine the hypothesis that the association of plasma apoB with T2D risk factors, hypertriglyceridemia (hyperTG), insulin resistance (IR), and hyperinsulinemia, was dependent on WAT dysfunction...
January 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28357654/human-adipocyte-differentiation-and-characterization-in-a-perfusion-based-cell-culture-device
#20
Yunxiao Liu, Patthara Kongsuphol, Sajay Bhuvanendran Nair Gourikutty, Qasem Ramadan
Adipocytes have gained significant attention recently, because they are not only functioning as energy storage but also as endocrine cells. Adipocytes secret various signaling molecules, including adiponectin, MCP-1, and IL-6, termed collectively as "adipokines". Adipokines regulate glucose metabolism, thereby play an important role in obesity, diabetes type 2, and other metabolic disorders. Conventionally, to study the secretory function, adipocytes are cultured in vitro in static conditions. However, static culturing condition falls short of mimicking the interstitial fluid flows in living systems...
September 2017: Biomedical Microdevices
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