keyword
MENU ▼
Read by QxMD icon Read
search

preadipocyte AND diabetes

keyword
https://www.readbyqxmd.com/read/29789508/dual-effects-of-metformin-on-adipogenic-differentiation-of-3t3-l1-preadipocyte-in-ampk-dependent-and-independent-manners
#1
Dian Chen, Ying Wang, Kaikai Wu, Xingya Wang
Metformin has been reported to have body weight lowering effects while treating type 2 diabetes. However, limited studies examined the effects of metformin on adipogenesis in vitro, and available data are inconclusive and contradictory. In this study, we examined the effects of a variety of concentrations of metformin on adipocyte differentiation of 3T3-L1 preadipocytes and found metformin exhibits a dual effect on adipogenesis. Metformin at lower concentrations (1.25⁻2.5 mM) significantly induced adipogenesis while at higher concentrations (5⁻10 mM) metformin significantly inhibited adipogenesis in 3T3-L1 cells...
May 23, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29789397/the-wnt-antagonist-and-secreted-frizzled-related-protein-5-implications-on-lipid-metabolism-inflammation-and-type-2-diabetes-mellitus
#2
Ling-Bin Liu, Xiao-Dong Chen, Xiang-Yu Zhou, Qing Zhu
Various reports have suggested that secreted frizzled-related protein 5 (SFRP5) plays a regulatory role in the processes of cellular proliferation and differentiation, by means of inactivating the Wnt/β-catenin signaling pathway. Recently, SFRP5 has been identified as an anti-inflammatory adipokine, which may be induced during preadipocyte proliferation, differentiation, and maturation. This review aims to identify the recent progress in the research and development of SFRP5 that can play a role in influencing lipid metabolism, inflammation and type 2 diabetes mellitus (T2DM)...
May 22, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29786968/p62-mediated-autophagy-affects-nutrition-dependent-insulin-receptor-substrate-1-dynamics-in-3t3-l1-preadipocytes
#3
Hirobumi Igawa, Akihiro Kikuchi, Hirofumi Misu, Kiyo-Aki Ishii, Shuichi Kaneko, Toshinari Takamura
AIMS/INTRODUCTION: Previous studies have shown that the organism's nutritional status changes the protein levels of insulin receptor substrate 1 (IRS-1) in a tissue-specific manner. Although the mechanisms underlying the regulation of IRS-1 in the nutrient-rich conditions associated with diabetes and insulin resistance have been well studied, those under nutrient-poor conditions remain unknown. The aim of this study was to investigate how IRS-1 protein levels change depending on the nutritional status of 3T3-L1 preadipocytes...
May 22, 2018: Journal of Diabetes Investigation
https://www.readbyqxmd.com/read/29775748/microrna-204-5p-regulates-3t3-l1-preadipocyte-proliferation-apoptosis-and-differentiation
#4
Jingjing Du, Peiwen Zhang, Mailin Gan, Xue Zhao, Yan Xu, Qiang Li, Yanzhi Jiang, Guoqing Tang, Mingzhou Li, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Obesity due to excessive lipid accumulation is closely associated with metabolic diseases such as type 2 diabetes, insulin resistance and inflammation. Therefore, a detailed understanding of the molecular mechanisms that underlie adipogenesis is crucial to develop treatments for diseases related to obesity. Here, we found that the microRNA-204-5p (miR-204-5p) was expressed at low levels in fat tissues from obese mice fed long-term with a high-fat diet (HFD). Overexpression or inhibition of miR-204-5p in vitro in 3T3-L1 preadipocytes significantly inhibited or promoted 3T3-L1 proliferation, respectively, an effect mediated by regulating cell proliferation factors...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29731242/therapeutic-effects-of-the-allosteric-protein-tyrosine-phosphatase-1b-inhibitor-ky-226-on-experimental-diabetes-and-obesity-via-enhancements-in-insulin-and-leptin-signaling-in-mice
#5
Yuma Ito, Masaki Fukui, Mamoru Kanda, Ko Morishita, Yoshimichi Shoji, Tatsuya Kitao, Eiichi Hinoi, Hiroaki Shirahase
The anti-diabetic and anti-obesity effects of the allosteric protein tyrosine phosphatase 1B (PTP1B) inhibitor 4-(biphenyl-4-ylmethylsulfanylmethyl)-N-(hexane-1-sulfonyl)benzoylamide (KY-226) were pharmacologically evaluated. KY-226 inhibited human PTP1B activity (IC50  = 0.28 μM), but did not exhibit peroxisome proliferator-activated receptor γ (PPARγ) agonist activity. In rodent preadipocytes (3T3-L1), KY-226 up to 10 μM had no effects on adipocyte differentiation, whereas pioglitazone, a PPARγ agonist, markedly promoted it...
March 6, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/29730658/differential-patterns-of-secreted-frizzled-related-protein-4-sfrp4-in-adipocyte-differentiation-adipose-depot-specificity
#6
Hua Guan, Yali Zhang, Shoucui Gao, Liang Bai, Sihai Zhao, Xian Wu Cheng, Jianglin Fan, Enqi Liu
BACKGROUND/AIMS: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that acts as soluble modulators of Wnt signaling. Given the substantial rise in obesity, depot-specific fat accumulation and its associated diseases like diabetes, it is important to understand the molecular basis of depot-specific adipocyte differentiation. In the current study, we investigated the expression of SFRP4 in both subcutaneous and visceral adipose tissue in terms of their differentiation...
April 28, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29720835/combination-of-garcinia-cambogia-extract-and-pear-pomace-extract-additively-suppresses-adipogenesis-and-enhances-lipolysis-in-3t3-l1-cells
#7
Kushal Sharma, Siwon Kang, Dalseong Gong, Sung-Hwa Oh, Eun-Young Park, Min-Ho Oak, Eunyoung Yi
Background: Inhibition of adipogenesis has been a therapeutic target for reducing obesity and obesity-related disorders such as diabetes, hypertension, atherosclerosis, and cancer. For decades, anti-adipogenic potential of many herbal extracts has been investigated. One example is Garcinia cambogia extract (GE) containing (-)-hydroxycitric acid as an active ingredient. GE is currently marketed as a weight loss supplement, used alone or with other ingredients. Pear pomace extract (PE), another natural product, has been also shown to have anti-adipogenic activity in a recent report...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29689639/establishment-and-characterization-of-a-primary-murine-adipose-tissue-chip
#8
Nida Tanataweethum, Adelyn Zelaya, Feipeng Yang, Ronald N Cohen, Eric M Brey, Abhinav Bhushan
Better experimental models are needed to enhance our understanding of metabolic regulation which is seen in obesity and metabolic disorders, such as type 2 diabetes. In vitro models based on microfluidics enable physiological representations of tissues with several advantages over conventional culture systems, such as perfused flow to better mimic the physiological environment. Although cell lines such as 3T3-L1 have been incorporated in microfluidic devices, murine primary preadipocytes have not been differentiated and maintained for long-term monitoring in these culture systems...
April 24, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29655677/an-in-vitro-and-in-vivo-study-of-a-4-herb-formula-on-the-management-of-diet-induced-metabolic-syndrome
#9
Elaine Wat, Yanping Wang, Ken Chan, Hon Wai Law, Chi Man Koon, Kit Man Lau, Ping Chung Leung, Choly Yan, Clara Bik San Lau
BACKGROUND: Metabolic syndrome is the cluster of risk factors that leads to increased episodes of cardiovascular disease (CVD). These risk factors include but are not limited to obesity, non-alcoholic fatty liver (NAFLD), dyslipidemia, and type 2 diabetes. Since the pathogenesis of metabolic syndrome has multiple metabolic origins, there is no single treatment for it. Pharmacological approaches consist of separate drugs which target at individual risk factors which pose various side effects...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29606342/what-can-brown-ing-do-for-you
#10
REVIEW
Amar M Singh, Stephen Dalton
Human stem cell-based models of thermogenic adipocytes provide an opportunity for the establishment of new therapeutics, modeling of disease mechanisms, and understanding of development. Pluripotent stem cells, adipose-derived stem cells/preadipocytes, and programming-reprogramming-based approaches have been used to develop cell-based platforms for drug screening and transplantable therapeutics in the metabolic disease arena. Here we provide a detailed overview of these approaches, the latest advances in this field, and the opportunities and shortcomings they present...
May 2018: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/29606113/md-1-a-poly-herbal-formulation-indicated-in-diabetes-mellitus-ameliorates-glucose-uptake-and-inhibits-adipogenesis-an-in-vitro-study
#11
Srivani Telapolu, Mangathayaru Kalachavedu, Alan Mathew Punnoose, Dwarakanath Bilikere
BACKGROUND: Type 2 Diabetes (T2D) is a polygenic disease requiring a multipronged therapeutic approach. In the current scenario, the use of polyherbals is increasing among the diabetics. MD-1, a poly herbal formulation is constituted as a mixture of six popular anti diabetic herbs, used in the management of Diabetes mellitus (DM). The physicochemical, biochemical and in vitro efficacy studies have been carried out to ascertain the possible mechanisms underlying the anti-diabetic action of MD-1...
April 2, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29593329/tp53inp2-regulates-adiposity-by-activating-%C3%AE-catenin-through-autophagy-dependent-sequestration-of-gsk3%C3%AE
#12
Montserrat Romero, Alba Sabaté-Pérez, Víctor A Francis, Ignacio Castrillón-Rodriguez, Ángels Díaz-Ramos, Manuela Sánchez-Feutrie, Xavier Durán, Manuel Palacín, José María Moreno-Navarrete, Birgit Gustafson, Ann Hammarstedt, José Manuel Fernández-Real, Joan Vendrell, Ulf Smith, Antonio Zorzano
Excessive fat accumulation is a major risk factor for the development of type 2 diabetes mellitus and other common conditions, including cardiovascular disease and certain types of cancer. Here, we identify a mechanism that regulates adiposity based on the activator of autophagy TP53INP2. We report that TP53INP2 is a negative regulator of adipogenesis in human and mouse preadipocytes. In keeping with this, TP53INP2 ablation in mice caused enhanced adiposity, which was characterized by greater cellularity of subcutaneous adipose tissue and increased expression of master adipogenic genes...
April 2018: Nature Cell Biology
https://www.readbyqxmd.com/read/29589999/biguanides-metformin-and-phenformin-generate-therapeutic-effects-via-amp-activated-protein-kinase-extracellular-regulated-kinase-pathways-in-an-in-vitro-model-of-graves-orbitopathy
#13
Ye Eon Han, Sena Hwang, Jin Hee Kim, Jung Woo Byun, Jin Sook Yoon, Eun Jig Lee
BACKGROUND: It was hypothesized that the biguanides metformin and phenformin, which are anti-hyperglycemic drugs used for diabetes mellitus, would have therapeutic effects in an in vitro model of Graves' orbitopathy (GO). Because adipogenesis, hyaluronan production, and inflammation are considered important in the pathogenesis of GO, this study aimed to determine the therapeutic effects and underlying mechanisms of biguanides on these parameters. METHODS: In vitro experiments were performed using primary cultured orbital fibroblasts from patients with GO...
April 2018: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/29550483/strigolactone-gr24-and-pinosylvin-attenuate-adipogenesis-and-inflammation-of-white-adipocytes
#14
Shalem Modi, Nagendra Yaluri, Tarja Kokkola
Obesity is characterized by excess fat accumulation in white adipose tissue, which triggers chronic low-grade inflammation through secretion of pro-inflammatory factors by the enlarged adipocytes and infiltrated macrophages. This affects glucose and lipid metabolism in adipose tissue, inducing type 2 diabetes. NAD+ -dependent deacetylase SIRT1 is known to inhibit adipogenesis through the regulation of the key adipogenic transcription factors, PPARγ and C/EBPα. SIRT1 activators such as resveratrol inhibit adipogenesis and exert anti-inflammatory responses in the adipose tissue...
May 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29526086/flavonoid-glycosides-from-fenugreek-seeds-regulate-glycolipid-metabolism-by-improving-mitochondrial-function-in-3t3-l1-adipocytes-in-vitro
#15
Guangxiang Luan, Yuwei Wang, Zhenhua Wang, Wenna Zhou, Na Hu, Gang Li, Honglun Wang
Fenugreek is a well known annual herb widely used in both medicine and food. Four flavonoid glycosides have been separated from fenugreek seeds in our previous study. In this study, the effects of the four flavonoid glycosides on regulating glycolipid metabolism and improving mitochondrial function were investigated. Isoorientin showed a very significant activity among these flavonoid glycosides. First, isoorientin decreased the accumulation of lipid droplets in 3T3-L1 preadipocytes by reducing the expression of adipokines including PPARγ, C/EBPα, and FAS...
March 28, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29514876/adipogenesis-in-fish
#16
REVIEW
Cristina Salmerón
White adipose tissue (AT) is the main lipid storage depot in vertebrates. Initially considered to be a simple lipid store, AT has recently been recognized as playing a role as an endocrine organ that is implicated in processes such as energy homeostasis and as a rich source of stem cells. Interest in adipogenesis has increased not only because of the prevalence of obesity, metabolic syndrome and type 2 diabetes in humans, but also in aquaculture because of the excessive fat deposition experienced in some cultured fish species, which may compromise both their welfare and their final product quality...
March 7, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29511320/preadipocytes-from-obese-humans-with-type-2-diabetes-are-epigenetically-reprogrammed-at-genes-controlling-adipose-tissue-function
#17
Emil Andersen, Lars Roed Ingerslev, Odile Fabre, Ida Donkin, Ali Altıntaş, Soetkin Versteyhe, Thue Bisgaard, Viggo B Kristiansen, David Simar, Romain Barrès
BACKGROUND: Deterioration of the adipogenic potential of preadipocytes may contribute to adipose tissue dysfunction in obesity and type 2 diabetes (T2D). Here, we hypothesized that extracellular factors in obesity epigenetically reprogram adipogenesis potential and metabolic function of preadipocytes. METHODS: The transcriptomic profile of visceral adipose tissue preadipocytes collected from Lean, Obese and Obese with T2D was assessed throughout in vitro differentiation using RNA sequencing...
February 20, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29497032/de-novo-adipocyte-differentiation-from-pdgfr%C3%AE-preadipocytes-protects-against-pathologic-visceral-adipose-expansion-in-obesity
#18
Mengle Shao, Lavanya Vishvanath, Napoleon C Busbuso, Chelsea Hepler, Bo Shan, Ankit X Sharma, Shiuhwei Chen, Xinxin Yu, Yu A An, Yi Zhu, William L Holland, Rana K Gupta
Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocyte hypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodeling are largely unknown. Here, we test the hypothesis that the potential to recruit new adipocytes from Pdgfrβ+ preadipocytes determines visceral WAT health in obesity. We manipulate levels of Pparg, the master regulator of adipogenesis, in Pdgfrβ+ precursors of adult mice. Increasing the adipogenic capacity of Pdgfrβ+ precursors through Pparg overexpression results in healthy visceral WAT expansion in obesity and adiponectin-dependent improvements in glucose homeostasis...
March 1, 2018: Nature Communications
https://www.readbyqxmd.com/read/29458019/raffinose-from-costus-speciosus-attenuates-lipid-synthesis-through-modulation-of-ppars-srebp1c-and-improves-insulin-sensitivity-through-pi3k-akt
#19
Padmanaban Muthukumaran, Gopal Thiyagarajan, Rajendran Arun Babu, Baddireddi Subhadra Lakshmi
Among several metabolic disorders, the pathogenesis of insulin resistance is considered to be multifactorial. Raffinose, an oligosaccharide isolated from the rhizome of Costus speciosus showed ≤50% inhibition of lipid accumulation in differentiated HepG2 and 3T3-L1 cells through exhibiting partial agonism to PPARγ, and, an enhanced secretion of adiponectin in 3T3-L1 adipocytes. Raffinose was also observed to attenuate the expression of SREBP1c, ACC and FAS which are involved in the fatty acid synthesis. A corresponding upregulation of PPARα and ACO involved in fatty acid oxidation was observed in steatotic HepG2 hepatocytes and 3T3-L1 adipocytes...
March 25, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29415997/downregulation-of-%C3%AE-1-4-galactosyltransferase-5-improves-insulin-resistance-by-promoting-adipocyte-commitment-and-reducing-inflammation
#20
Shu-Fen Li, Cui-Song Zhu, Yu-Meng Wang, Xin-Xin Xie, Liu-Ling Xiao, Zhi-Chun Zhang, Qi-Qun Tang, Xi Li
Protein glycosylation is an important post-translational modification. Aberrant glycosylation has been implicated in many diseases because of associated changes in protein distribution and biological function. We showed that the expression of β1, 4-galactosyltransferase 5 (B4GalT5) was positively correlated with diabetes and obesity. In vivo, B4GalT5 knockdown in subcutaneous adipose tissue alleviated insulin resistance and adipose tissue inflammation, and increased adipogenesis in high-fat diet (HFD)-fed mice and ob/ob mice...
February 7, 2018: Cell Death & Disease
keyword
keyword
25950
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"