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https://www.readbyqxmd.com/read/28548078/associated-variables-of-myositis-in-systemic-lupus-erythematosus-a-cross-sectional-study
#1
Yan Liang, Rui-Xue Leng, Hai-Feng Pan, Dong-Qing Ye
BACKGROUND This study aimed to estimate the point prevalence of myositis and identify associated variables of myositis in systemic lupus erythematosus (SLE). MATERIAL AND METHODS Clinical date of patients hospitalized with lupus at the First Affiliated Hospital of Anhui Medical University and Anhui Provincial Hospital were collected. Patients were defined as having myositis if they reported the presence of persistent invalidating muscular weakness combined with increased levels of creatine phosphokinase (CPK) and abnormal electromyography (EMG)...
May 26, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28542332/non-canonical-binding-interactions-of-the-rna-recognition-motif-rrm-domains-of-p34-protein-modulate-binding-within-the-5s-ribonucleoprotein-particle-5s-rnp
#2
Anyango D Kamina, Noreen Williams
RNA binding proteins are involved in many aspects of RNA metabolism. In Trypanosoma brucei, our laboratory has identified two trypanosome-specific RNA binding proteins P34 and P37 that are involved in the maturation of the 60S subunit during ribosome biogenesis. These proteins are part of the T. brucei 5S ribonucleoprotein particle (5S RNP) and P34 binds to 5S ribosomal RNA (rRNA) and ribosomal protein L5 through its N-terminus and its RNA recognition motif (RRM) domains. We generated truncated P34 proteins to determine these domains' interactions with 5S rRNA and L5...
2017: PloS One
https://www.readbyqxmd.com/read/28536288/cnot4-mediated-ubiquitination-of-influenza-a-virus-nucleoprotein-promotes-viral-rna-replication
#3
Yu-Chen Lin, King-Song Jeng, Michael M C Lai
Influenza A virus (IAV) RNA segments are individually packaged with viral nucleoprotein (NP) and RNA polymerases to form a viral ribonucleoprotein (vRNP) complex. We previously reported that NP is a monoubiquitinated protein which can be deubiquitinated by a cellular ubiquitin protease, USP11. In this study, we identified an E3 ubiquitin ligase, CNOT4 (Ccr4-Not transcription complex subunit 4), which can ubiquitinate NP. We found that the levels of viral RNA, protein, viral particles, and RNA polymerase activity in CNOT4 knockdown cells were lower than those in the control cells upon IAV infection...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28524231/comparison-of-pre-ribosomal-rna-processing-pathways-in-yeast-plant-and-human-cells-focus-on-coordinated-action-of-endo-and-exoribonucleases
#4
REVIEW
Rafal Tomecki, Pawel J Sikorski, Monika Zakrzewska-Placzek
Proper regulation of ribosome biosynthesis is mandatory for cellular adaptation, growth and proliferation. Ribosome biogenesis is the most energetically demanding cellular process, which requires tight control. Abnormalities in ribosome production have severe consequences, including developmental defects in plants and genetic diseases (ribosomopathies) in humans. One of the processes occurring during eukaryotic ribosome biogenesis is processing of the ribosomal RNA precursor molecule (pre-rRNA), synthesized by RNA polymerase I, into mature rRNAs...
May 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28505348/implication-of-the-box-c-d-snornp-assembly-factor-rsa1p-in-u3-snornp-assembly
#5
Benjamin Rothé, Xavier Manival, Nicolas Rolland, Christophe Charron, Véronique Senty-Ségault, Christiane Branlant, Bruno Charpentier
The U3 box C/D snoRNA is one key element of 90S pre-ribosome. It contains a 5΄ domain pairing with pre-rRNA and the U3B/C and U3C΄/D motifs for U3 packaging into a unique small nucleolar ribonucleoprotein particle (snoRNP). The RNA-binding protein Snu13/SNU13 nucleates on U3B/C the assembly of box C/D proteins Nop1p/FBL and Nop56p/NOP56, and the U3-specific protein Rrp9p/U3-55K. Snu13p/SNU13 has a much lower affinity for U3C΄/D but nevertheless forms on this motif an RNP with box C/D proteins Nop1p/FBL and Nop58p/NOP58...
May 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28503153/investigating-engineered-ribonucleoprotein-particles-to-improve-oral-rnai-delivery-in-crop-insect-pests
#6
François-Xavier Gillet, Rayssa A Garcia, Leonardo L P Macedo, Erika V S Albuquerque, Maria C M Silva, Maria F Grossi-de-Sa
Genetically modified (GM) crops producing double-stranded RNAs (dsRNAs) are being investigated largely as an RNA interference (RNAi)-based resistance strategy against crop insect pests. However, limitations of this strategy include the sensitivity of dsRNA to insect gut nucleases and its poor insect cell membrane penetration. Working with the insect pest cotton boll weevil (Anthonomus grandis), we showed that the chimeric protein PTD-DRBD (peptide transduction domain-dsRNA binding domain) combined with dsRNA forms a ribonucleoprotein particle (RNP) that improves the effectiveness of the RNAi mechanism in the insect...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28500298/zbp1-phosphorylation-at-serine-181-regulates-its-dendritic-transport-and-the-development-of-dendritic-trees-of-hippocampal-neurons
#7
Anna S Urbanska, Aleksandra Janusz-Kaminska, Katarzyna Switon, Alicia L Hawthorne, Malgorzata Perycz, Malgorzata Urbanska, Gary J Bassell, Jacek Jaworski
Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA binding proteins ensure mRNA silencing and provide a link to molecular motors. ZBP1 is a component of RNP transport particles and is known for its role in the local translation of β-actin mRNA. Its binding to mRNA is regulated by tyrosine 396 phosphorylation, and this particular modification was shown to be vital for axonal growth and dendritic branching...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28476952/specific-rnp-capture-with-antisense-lna-dna-mixmers
#8
Birgit Maria Rogell, Bernd Fischer, Mandy Rettel, Jeroen Krijgsveld, Alfredo Castello, Matthias W Hentze
RNA-binding proteins (RBPs) play essential roles in RNA biology, responding to cellular and environmental stimuli to regulate gene expression. Important advances have helped to determine the (near) complete repertoires of cellular RBPs. However, identification of RBPs associated with specific transcripts remains a challenge. Here, we describe "specific ribonucleoprotein (RNP) capture", a versatile method for the determination of the proteins bound to specific transcripts in vitro and in cellular systems. Specific RNP capture employs UV irradiation to covalently stabilize protein-RNA interactions taking place at "zero distance"...
May 5, 2017: RNA
https://www.readbyqxmd.com/read/28468076/-recombinant-expression-of-hantaan-virus-protein-n-with-application-of-western-blot-in-detecting-anti-hantavirus-antibody
#9
P P Yao, F Xu, Y S Sun, Z R Yang, Y Zhang, M Yue, H P Zhu
Objective: S gene of hantavirus(HV) was expressed in insect cells by genetic engineering technology. The expression product of S gene was used as antigen to detect anti-HV specific antibody IgG in serum. Methods: Gene encoding NP of the strain HV-Z10 was amplified by PCR and then its eukaryotic expression system rBAC-Z10S-TN was constructed by using the routine genetic engineering method. SDS-PAGE was applied to measure the expression of rNP.Ion-exchange plus Ni-NTA-affinity chromatography was performed to purify the recombinant product...
April 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28462641/the-utility-of-the-radionuclide-probe-in-parathyroidectomy-for-primary-hyperparathyroidism
#10
M S Lim, M Jinih, C H Ngai, N M Foley, H P Redmond
INTRODUCTION Parathyroidectomy is the definitive treatment for primary hyperparathyroidism but the intraoperative identification of adenomas is challenging. The aim of this study was to evaluate the utility of a radionuclide probe (RNP) in addition to intraoperative parathyroid hormone ( IOPTH) measurement as an intraoperative diagnostic adjunct in patients undergoing parathyroidectomy for primary hyperparathyroidism. METHODS This was a retrospective cohort study of patients treated between 2004 and 2015 in a university affiliated teaching hospital...
May 2017: Annals of the Royal College of Surgeons of England
https://www.readbyqxmd.com/read/28460336/application-of-surface-enhanced-raman-spectroscopy-as-a-diagnostic-system-for-hypersialylated-metastatic-cancers
#11
Babita Shashni, Yukichi Horiguchi, Kosuke Kurosu, Hitoshi Furusho, Yukio Nagasaki
Early diagnosis of metastatic cancers could greatly limit the number of cancer-associated deaths. Aberrant surface expression of sialic acid (hypersialylation) on tumors correlating with metastatic incidence and its involvement in tumorigenesis and progression is widely reported; hence detection of hypersialylated tumors may be an effective strategy to identify metastatic cancers. We herein report on the application of phenylboronic acid-installed PEGylated gold nanoparticles coupled with Toluidine blue O (T/BA-GNPs) as SERS probes to target surface sialic acid (N-acetylneuraminic acid, Neu5Ac)...
April 21, 2017: Biomaterials
https://www.readbyqxmd.com/read/28457979/isolation-of-yeast-and-mammalian-stress-granule-cores
#12
Joshua R Wheeler, Saumya Jain, Anthony Khong, Roy Parker
Stress granules are dynamic, conserved RNA-protein (RNP) assemblies that form when translation is limiting; and are related to pathological aggregates in degenerative disease. Mammalian stress granules are comprised of two structures - an unstable shell and more stable cores. Herein we describe methodology for isolation of stress granule cores from both yeast and mammalian cells. The protocol consists of first enriching for stress granule cores using centrifugation and then further purifying stress granule cores using immunoprecipitation...
April 27, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28439670/residual-recurrent-nerve-paralysis-after-esophagectomy-is-associated-with-preoperative-lower-serum-albumin
#13
Makoto Miyamoto, Yoshiki Kobayashi, Eri Miyata, Tomofumi Sakagami, Masao Yagi, Akira Kanda, Taku Michiura, Koichi Tomoda
Esophagectomy for esophageal cancer is invasive thoracic surgery with a high incidence rate of postoperative complications and prolongation of hospitalization, even if the standardized clinical pathway improves the outcome (mortality and morbidity). Postoperative recurrent nerve paralysis (RNP) is related to respiratory complications concomitant with prolonged hospitalization. However, it has not been elucidated which factors affect the incidence and recovery of RNP. To detect the predictive factor for postoperative RNP, we focused on preoperative serum albumin...
April 24, 2017: Dysphagia
https://www.readbyqxmd.com/read/28412356/leptomycin-b-alters-the-subcellular-distribution-of-crm1-exportin-1
#14
Khatera Rahmani, David A Dean
CRM1 (chromosome maintenance region 1, Exportin 1) binds to nuclear export signals and is required for nucleocytoplasmic transport of a large variety of proteins and RNP complexes. Leptomycin B (LMB), the first specific inhibitor of CRM1 identified, binds covalently to cysteine 528 in the nuclear export signal binding region of CRM1 leading to the inhibition of protein nuclear export. Although the biochemical mechanisms of action of CRM1 inhibitors such as LMB are well studied, the subcellular effects of inhibition on CRM1 are unknown...
April 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28410976/genome-editing-via-delivery-of-cas9-ribonucleoprotein
#15
Mark DeWitt, Jacob E Corn, Dana Carroll
The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a powerful and general approach to genome editing. Here we outline methods to produce and deliver Cas9 RNPs. A donor DNA carrying desired sequence changes can also be included to program precise sequence introduction or replacement...
April 11, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28409522/serological-biomarkers-as-risk-factors-of-sle-associated-pulmonary-arterial-hypertension-a-systematic-review-and-meta-analysis
#16
J Wang, J Qian, Y Wang, J Zhao, Q Wang, Z Tian, M Li, X Zeng
Objective This article aims to determine the serological biomarkers which can be considered as risk factors of systemic lupus erythematosus (SLE)-associated pulmonary arterial hypertension by a systematic review and meta-analysis. Methods This study was conducted in accordance with the PRISMA statement. The search database included MEDLINE, EMBASE, Cochrane Library and Scopus. The Newcastle-Ottawa scale was used for the quality assessment. The odds ratio was the primary measure of effect of the risk factors...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28408981/rna-and-proteins-mutual-respect
#17
REVIEW
Kathleen B Hall
Proteins and RNA are often found in ribonucleoprotein particles (RNPs), where they function in cellular processes to synthesize proteins (the ribosome), chemically modify RNAs (small nucleolar RNPs), splice pre-mRNAs (the spliceosome), and, on a larger scale, sequester RNAs, degrade them, or process them (P bodies, Cajal bodies, and nucleoli). Each RNA-protein interaction is a story in itself, as both molecules can change conformation, compete for binding sites, and regulate cellular functions. Recent studies of Xist long non-coding RNP, the U4/5/6 tri-small nuclear RNP complex, and an activated state of a spliceosome reveal new features of RNA interactions with proteins, and, although their stories are incomplete, they are already fascinating...
2017: F1000Research
https://www.readbyqxmd.com/read/28396624/granulostasis-protein-quality-control-of-rnp-granules
#18
REVIEW
Simon Alberti, Daniel Mateju, Laura Mediani, Serena Carra
Ribonucleoprotein (RNP) granules transport, store, or degrade messenger RNAs, thereby indirectly regulating protein synthesis. Normally, RNP granules are highly dynamic compartments. However, because of aging or severe environmental stress, RNP granules, in particular stress granules (SGs), convert into solid, aggregate-like inclusions. There is increasing evidence that such RNA-protein inclusions are associated with several age-related neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), fronto-temporal dementia (FTD) and Alzheimer's disease (AD)...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28394238/anti-ribosomal-p-antibody-a-multicenter-study-in-childhood-onset-systemic-lupus-erythematosus-patients
#19
C C M Valões, B C Molinari, A C G Pitta, N W S Gormezano, S C L Farhat, K Kozu, A M E Sallum, S Appenzeller, A P Sakamoto, M T Terreri, R M R Pereira, C S Magalhães, J C O A Ferreira, C M Barbosa, F H Gomes, E Bonfá, C A Silva
Objectives Anti-ribosomal P protein (anti-P) autoantibodies are highly specific for systemic lupus erythematosus (SLE). However, the evaluation of this autoantibody in childhood-onset SLE (cSLE) populations has been limited to a few small series, hampering the interpretation of the clinical and laboratorial associations. Therefore, the objective of this multicenter cohort study was to evaluate demographic, clinical/laboratorial features, and disease damage score in cSLE patients with and without the presence of anti-P antibody...
April 2017: Lupus
https://www.readbyqxmd.com/read/28389661/crispr-cas9-mediated-pd-1-disruption-enhances-anti-tumor-efficacy-of-human-chimeric-antigen-receptor-t-cells
#20
Levi J Rupp, Kathrin Schumann, Kole T Roybal, Rachel E Gate, Chun J Ye, Wendell A Lim, Alexander Marson
Immunotherapies with chimeric antigen receptor (CAR) T cells and checkpoint inhibitors (including antibodies that antagonize programmed cell death protein 1 [PD-1]) have both opened new avenues for cancer treatment, but the clinical potential of combined disruption of inhibitory checkpoints and CAR T cell therapy remains incompletely explored. Here we show that programmed death ligand 1 (PD-L1) expression on tumor cells can render human CAR T cells (anti-CD19 4-1BBζ) hypo-functional, resulting in impaired tumor clearance in a sub-cutaneous xenograft model...
April 7, 2017: Scientific Reports
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