Read by QxMD icon Read


Taylor Wand, Mike Fang, Christina Chen, Nathan Hardy, J Philip McCoy, Bogdan Dumitriu, Neal S Young, Angélique Biancotto
Abnormal telomere lengths have been linked to cancer and other hematologic disorders. Determination of mean telomere content (MTC) is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Here, we compared a quantitative Polymerase Chain Reaction approach (qPCR) and a flow cytometric approach, fluorescence in situ hybridization (Flow-FISH), to evaluate telomere content distribution in total patient peripheral blood mononuclear cells or specific cell populations...
September 22, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Santiago Roura, Marco A Fernández, Elena Elchinova, Iris Teubel, Gerard Requena, Roser Cabanes, Josep Lupón, Antoni Bayes-Genis
Conventional analytical methods to determine telomere length (TL) have been replaced by more precise and reproducible procedures, such as fluorescence in situ hybridization coupled with flow cytometry (flow-FISH). However, simultaneous measurement of TL and cell phenotype remains difficult. Relatively expensive and time-consuming cell-sorting purification is needed to counteract the loss, due to stringent FISH conditions, of prehybridization fluorescence by the organic fluorochromes conventionally used in the phenotyping step...
September 12, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
Shahinaz M Gadalla, Payal P Khincha, Hormuzd A Katki, Neelam Giri, Jason Y Y Wong, Stephen Spellman, Jack A Yanovski, Joan C Han, Immaculata De Vivo, Blanche P Alter, Sharon A Savage
BACKGROUND: Telomere length <1st percentile-for-age in leukocyte subsets by flow cytometry with fluorescence in situ hybridization (flow FISH) is highly sensitive and specific in diagnosing patients with dyskeratosis congenita (DC), a telomere biology disorder. METHODS: We evaluated the clinical utility of the high-throughput quantitative real-time PCR (qPCR) relative telomere length (RTL) measurement as a diagnostic test for DC in patients with a priori clinical and/or genetic DC diagnoses...
July 2016: Molecular Genetics & Genomic Medicine
R Ranjbaran, M A Okhovat, M Abbasi, L Moezzi, F Aboualizadeh, Z Amidzadeh, H A Golafshan, A Behzad-Behbahani, S Sharifzadeh
INTRODUCTION: We assessed the feasibility of flow cytometry-fluorescent in situ hybridization technique in the detection of translocated mRNA in the cytoplasm of human peripheral blood nucleated cells. It is assumed that this assay can be applied as a diagnostic method in the detection of chromosomal translocation which commonly occurs in hematologic malignancies. METHODS: KCL-22 cell line and white blood cells from 21 CML patients were recruited in the study. Cells were isolated and fixed...
August 2016: International Journal of Laboratory Hematology
Sourindra N Maiti
Natural killer (NK) cells are a subset of cytotoxic lymphocytes that play a critical role in innate immune surveillance against infections and tumors through cytokine secretion and target cell lysis. NK cells function without any need for prior antigen exposure. Thus, more recently NK cells are considered a promising source of lymphocytes for adoptive tumor therapy. However, because NK cells represent only a small lymphocyte fraction, expand poorly ex vivo, and have limited life spans, clinical scale generation of NK cells for tumor immunotherapy was a challenging issue...
2016: Methods in Molecular Biology
Kimberly L Dibble, Charles B Yackulic, Theodore A Kennedy, Phaedra Budy
Rainbow and brown trout have been intentionally introduced into tailwaters downriver of dams globally and provide billions of dollars in economic benefits. At the same time, recruitment and maximum length of trout populations in tailwaters often fluctuate erratically, which negatively affects the value of fisheries. Large recruitment events may increase dispersal downriver where other fish species may be a priority (e.g., endangered species). There is an urgent need to understand the drivers of trout population dynamics in tailwaters, in particular the role of flow management...
December 2015: Ecological Applications: a Publication of the Ecological Society of America
Carola I Weidner, Qiong Lin, Carina Birkhofer, Uwe Gerstenmaier, Andrea Kaifie, Martin Kirschner, Heiko Bruns, Stefan Balabanov, Arne Trummer, Clemens Stockklausner, Britta Höchsmann, Hubert Schrezenmeier, Marcin Wlodarski, Jens Panse, Tim H Brümmendorf, Fabian Beier, Wolfgang Wagner
Dyskeratosis congenita (DKC) is associated with impaired telomere maintenance and with clinical features of premature aging. In this study, we analysed global DNA methylation (DNAm) profiles of DKC patients. Age-associated DNAm changes were not generally accelerated in DKC, but there were significant differences to DNAm patterns of healthy controls, particularly in CpG sites related to an internal promoter region of PR domain containing 8 (PRDM8). Notably, the same genomic region was also hypermethylated in aplastic anemia (AA) - another bone marrow failure syndrome...
March 8, 2016: Oncotarget
Maurizio Carbonari
Formamide has long been one of the most widely used reagents in the study of nucleic acids. However, the use of formamide for treating cells to be analyzed by flow cytometry is a recent development and is restricted to measuring telomere lengths by flow-FISH. In this field, we have published several papers in order to observe the effects of formamide treatment on cells at room temperature. We therefore discovered that, with suitable modifications, a short and simple incubation in this ionizing solvent facilitates cell cycle analysis by flow cytometry, equivalent or superior to that obtained with treatments in alcohol, acetone or detergent in hypotonic solution...
May 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Gloria Bua, Elisabetta Manaresi, Francesca Bonvicini, Giorgio Gallinella
The pathogenic Parvovirus B19 (B19V) is characterized by a strict adaptation to erythroid progenitor cells (EPCs), a heterogeneous population of differentiating cells with diverse phenotypic and functional properties. In our work, we studied the dynamics of B19V infection in EPCs in dependence on the cell differentiation stage, in terms of distribution of infected cells, synthesis of viral nucleic acids and production of infectious virus. EPCs at early differentiation stage led to an abortive infection, without viral genome replication and a very low transcriptional activity...
2016: PloS One
Fabíola Carvalho Santos, Romain Guyot, Cacilda Borges do Valle, Lucimara Chiari, Vânia Helena Techio, Pat Heslop-Harrison, André Luís Laforga Vanzela
Like other eukaryotes, the nuclear genome of plants consists of DNA with a small proportion of low-copy DNA (genes and regulatory sequences) and very abundant DNA sequence motifs that are repeated thousands up to millions of times in the genomes including transposable elements (TEs) and satellite DNA. Retrotransposons, one class of TEs, are sequences that amplify via an RNA intermediate and reinsert into the genome, are often the major fraction of a genome. Here, we put research on retrotransposons into the larger context of plant repetitive DNA and genome behaviour, showing features of genome evolution in a grass genus, Brachiaria, in relation to other plant species...
September 2015: Chromosome Research
Juliette Gaillard, Marielle Thomas, Angélique Lazartigues, Bénilde Bonnefille, Christelle Pallez, Xavier Dauchy, Cyril Feidt, Damien Banas
Barrage fishponds may represent a significant surface water area in some French regions. Knowledge on their effect on water resources is therefore necessary for the development of appropriate water quality management plans at the regional scale. Although there is much information on the nutrient removal capacity of these water bodies, little attention has been paid to other agricultural contaminants such as pesticides. The present paper reports the results of a 1-year field monitoring of pesticide concentrations and water flows measured upstream and downstream from a fishpond in North East France to evaluate its capacity in reducing pesticide loads...
January 2016: Environmental Science and Pollution Research International
Knut Marius Myrvold, Brian P Kennedy
Self-thinning patterns are frequently used to describe density dependence in populations on timescales shorter than the organism's life span and have been used to infer carrying capacity of the environment. Among mobile animals, this concept has been used to document density dependence in stream salmonids, which compete over access to food and space. The carrying capacity, growth conditions, and initial cohort sizes often vary between streams and stream sections, which would influence the onset and strength of the density dependence...
August 2015: Ecology and Evolution
M E Barnes, C R Hewitt, T M Parker
This study examined occupational noise within two rearing facilities at a production fish hatchery and evaluated two simple noise reduction techniques. Ambient noise levels in the hatchery tank room ranged from 50 dB in the absence of flowing water to over 73 dB when water was flowing to all 35 tanks under typical hatchery operating procedures. Covering the open standpipes did not significantly reduce noise levels. However, placing partial tank covers over the top of the tanks above the water inlet significantly reduced noise levels, both with and without the use of standpipe covers...
July 2015: Journal of Agricultural Safety and Health
Lidia Marszał, Dagmara Błońska
The aim of the current study was to examine whether environmental conditions may explain interpopulation variation in fecundity and egg size of the spirlin from two rivers of the Vistula River basin. The obtained results indicated that the reproductive performance, including the gonadosomatic index, was similar in both rivers and fecundity increased with total length of females. The observed differences in water temperatures and flow speed between the rivers were too small to cause discernible differences in the reproductive performance of fish...
September 2015: Reproductive Biology
Khaled A Abdel-Moein, Hossam Saeed, Ahmed Samir
Helicobacter pylori is one of the most common human pathogens worldwide with serious clinical outcomes. Although, H. pylori is a major water-borne pathogen, its occurrence in fish is still unknown. This leads us to conduct the current study in order to clarify this point and to investigate the potential role of fish in the epidemiology of H. pylori. For this purpose, fecal samples were obtained from 315 fish from different species and were caught from various aquatic environments at different localities in Egypt...
December 2015: Acta Tropica
Ghaleb Elyamany, Ali Matar Alzahrani, Eman Al Mussaed, Hassan Aljasem, Sultan Alotaibi, Hatem Elghezal
The recent World Health Organization (WHO) classification defines mantle cell lymphoma (MCL) as a distinct entity characterized by a unique immunophenotype and a molecular hallmark of chromosomal translocation t(11;14)(q13;q32). We report an unusual case of an advanced stage of CD5 negative MCL with a blastoid variant with a massive bone marrow (BM) necrosis as an initial presenting feature, with no adenopathy or hepatosplenomegaly. The pathologic features showed blastoid variant of MCL and flow cytometry showed that the tumor cells were CD5-, CD19+, CD20+, FMC-7+, CD23-, and lambda light chain restricted...
2015: Case Reports in Hematology
Elina Laanto, Jaana K H Bamford, Janne J Ravantti, Lotta-Riina Sundberg
Flavobacterium columnare, the causative agent of columnaris disease in fish, causes millions of dollars of losses in the US channel catfish industry alone, not to mention aquaculture industry worldwide. Novel methods are needed for the control and treatment of bacterial diseases in aquaculture to replace traditionally used chemotherapies. A potential solution could be the use of phages, i.e., bacterial viruses, host-specific and self-enriching particles that can be can easily distributed via water flow. We examined the efficacy of phages to combat columnaris disease...
2015: Frontiers in Microbiology
M I Ríos-Pulgarín, M Barletta, N J Mancera-Rodríguez
The seasonal and interannual changes of the fish assemblage in the main channel and littoral zone of the Guarinó River, a torrential system located in the Colombian Andean foothills, were examined in relation to the physical and chemical environmental changes associated with the hydrological cycle and the El Niño-La Niña-Southern Oscillation (ENSO) between 2007 and 2010. Four samplings per year (in dry season and high water) were performed. Environmental variables (temperature, pH, conductivity, turbidity, oxygen, total nitrogen, orthophosphate, depth and flow rate) were contrasted with ANOVAs and a discriminant analysis to establish temporal patterns...
July 2016: Journal of Fish Biology
Jenna E Cavallin, Kathleen M Jensen, Michael D Kahl, Daniel L Villeneuve, Kathy E Lee, Anthony L Schroeder, Joe Mayasich, Evan P Eid, Krysta R Nelson, Rebecca Y Milsk, Brett R Blackwell, Jason P Berninger, Carlie A LaLone, Chad Blanksma, Terri Jicha, Colleen Elonen, Rodney Johnson, Gerald T Ankley
Wastewater treatment plant (WWTP) effluents are known contributors of chemical mixtures into the environment. Of particular concern are endocrine-disrupting compounds, such as estrogens, which can affect the hypothalamic-pituitary-gonadal axis function in exposed organisms. The present study examined reproductive effects in fathead minnows exposed for 21 d to a historically estrogenic WWTP effluent. Fathead minnow breeding pairs were held in control water or 1 of 3 effluent concentrations (5%, 20%, and 100%) in a novel onsite, flow-through system providing real-time exposure...
March 2016: Environmental Toxicology and Chemistry
F Huhn, W M van Rees, M Gazzola, D Rossinelli, G Haller, P Koumoutsakos
Undulatory swimmers flex their bodies to displace water, and in turn, the flow feeds back into the dynamics of the swimmer. At moderate Reynolds number, the resulting flow structures are characterized by unsteady separation and alternating vortices in the wake. We use the flow field from simulations of a two-dimensional, incompressible viscous flow of an undulatory, self-propelled swimmer and detect the coherent Lagrangian vortices in the wake to dissect the driving momentum transfer mechanisms. The detected material vortex boundary encloses a Lagrangian control volume that serves to track back the vortex fluid and record its circulation and momentum history...
August 2015: Chaos
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"