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Diabetes cornea

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https://www.readbyqxmd.com/read/28033432/association-between-endothelial-cell-density-and-transplant-suitability-of-corneal-tissue-with-type-1-and-type-2-diabetes
#1
Jordan A Margo, Wuqaas M Munir, Clayton H Brown, Caroline K Hoover
Importance: The rate of types 1 and 2 diabetes in the United States is increasing. The effect of diabetes on corneal donor tissue is unknown. Objectives: To determine the association between endothelial cell density and suitability for transplantation in cornea donors with type 1 or 2 diabetes and determine the effect of diabetes on technician-induced endothelial damage during cornea donor tissue processing. Design, Setting, and Participants: Donor information was obtained from the SightLife Eye Bank for donors from June 1, 2012, to June 30, 2015...
December 29, 2016: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28025096/early-vs-late-intervention-of-high-fat-low-dose-streptozotocin-treated-c57bl-6j-mice-with-enalapril-%C3%AE-lipoic-acid-menhaden-oil-or-their-combination-effect-on-diabetic-neuropathy-related-endpoints
#2
Matthew S Yorek, Alexander Obrosov, Hanna Shevalye, Lawrence J Coppey, Randy H Kardon, Mark A Yorek
We have previously demonstrated that enalapril, α-lipoic acid and menhaden (fish) oil has potential as a treatment for diabetic peripheral neuropathy. In this study we sought to determine the efficacy of these treatments individually or in combination on multiple neuropathic endpoints in a high fat fed low dose streptozotocin treated mouse, a model of type 2 diabetes, following early or late intervention. Four or twelve weeks after the onset of hyperglycemia, diabetic mice were treated with enalapril, α-lipoic acid, menhaden oil or their combination for 12 weeks...
December 23, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/28005998/establishment-of-a-3d-in-vitro-model-to-accelerate-the-development-of-human-therapies-against-corneal-diabetes
#3
Shrestha Priyadarsini, Akhee Sarker-Nag, Tyler G Rowsey, Jian-Xing Ma, Dimitrios Karamichos
PURPOSE: To establish an in vitro model that would mirror the in vivo corneal stromal environment in diabetes (DM) patients. METHODS: Human corneal fibroblasts from Healthy (HCFs), Type 1DM (T1DM) and Type 2DM (T2DM) donors were isolated and cultured for 4 weeks with Vitamin C stimulation in order to allow for extracellular matrix (ECM) secretion and assembly. RESULTS: Our data indicated altered cellular morphology, increased cellular migration, increased ECM assembly, and severe mitochondrial damage in both T1DM and T2DMs when compared to HCFs...
2016: PloS One
https://www.readbyqxmd.com/read/27999549/the-role-of-connexins-in-wound-healing-and-repair-novel-therapeutic-approaches
#4
REVIEW
Pui Wong, Teresa Tan, Catherine Chan, Victoria Laxton, Yin Wah Fiona Chan, Tong Liu, Wing Tak Wong, Gary Tse
Gap junctions are intercellular proteins responsible for mediating both electrical and biochemical coupling through the exchange of ions, second messengers and small metabolites. They consist of two connexons, with (one) connexon supplied by each cell. A connexon is a hexamer of connexins and currently more than 20 connexin isoforms have been described in the literature thus far. Connexins have a short half-life, and therefore gap junction remodeling constantly occurs with a high turnover rate. Post-translational modification, such as phosphorylation, can modify their channel activities...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27984834/-ophthalmological-monitoring-of-diabetic-neuropathy-in-a-mouse-model
#5
S Baltrusch
Patients with diabetes mellitus suffer from late damage, including microvascular and macrovascular complications and diabetic polyneuropathy, even though blood glucose is well controlled and the HbA1c value normalised. Peripheral nerves are affected, and when these are damaged, this can result in disturbed sensation and pain. Such nerves are mainly Aδ und C fibres, which are present in the skin, but also in the subbasal nerve plexus of the cornea. Imaging of the subbasal nerve plexus using microscopic techniques allows a very early and objective view of neuropathy, which is only possible invasively by skin biopsy...
December 2016: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/27984364/age-stratified-analysis-of-diabetes-and-pseudophakia-effects-on-corneal-endothelial-cell-density-a-retrospective-eye-bank-study
#6
Yicheng Chen, Sean W Tsao, Moonseong Heo, Patrick K Gore, Mitchell D McCarthy, Roy S Chuck, Prabjot Channa
PURPOSE: To perform an age-stratified analysis of the effect of diabetes and pseudophakia on corneal endothelial cell density (ECD). METHODS: This is a comparative analysis of donor characteristics from data supplied by the Lions Eye Institute for Transplant and Research on tissue harvested from July 1, 2007, through May 23, 2014. The mixed-effects model was used to compare age-adjusted mean corneal ECD between donors with and without diabetes. RESULTS: A total of 20,026 nondiabetic donor eyes and 13,617 diabetic donor eyes were included in this study...
December 14, 2016: Cornea
https://www.readbyqxmd.com/read/27978558/nerve-growth-factor-attenuates-apoptosis-and-inflammation-in-the-diabetic-cornea
#7
Jin Hyoung Park, Soon-Suk Kang, Jae Yong Kim, Hungwon Tchah
Purpose: To examine the effects of nerve growth factor (NGF) on apoptosis and inflammation in the diabetic cornea. Methods: To investigate the effects of NGF on glucose-induced apoptosis, we stained human corneal epithelial cells (HCECs) for annexin-V and propidium iodide (PI), and measured expression of cleaved caspase-3 and the Bcl-2-associated X protein (BAX). Moreover, to examine the effects of NGF on inflammation, we quantified the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) using multiplex cytokine analysis, and analyzed nuclear factor-κB (NF-κB) activation and NF-κ-B inhibitor α (IκBα) degradation using Western blot analysis...
December 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27930505/increased-membrane-localization-of-pannexin1-in-human-corneal-synaptosomes-causes-enhanced-stimulated-atp-release-in-chronic-diabetes-mellitus
#8
Hao Cui, Ying Liu, Limin Qin, Liqiang Wang, Yifei Huang
In the present study, we investigated the potential changes in the corneal nerve terminals in non-insulin-dependent diabetes mellitus of moderate duration. The dissected corneas were subjected to a protocol of ultracentrifugation to obtain synaptosomes of sensory nerve terminals. Within these nerve varicosities, 2 major mechanisms were examined, viz., alterations of the mechanosensitive channel pannexin1 and ATP release on stimulation of these terminals. We hypothesized that altered cellular location and function of the pannexin channel may contribute to altered mechanosensitivity of the cornea, which in turn may affect wound healing and primary visual function of the cornea...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27877085/the-evolving-functions-of-autophagy-in-ocular-health-a-double-edged-sword
#9
REVIEW
Peiwei Chai, Hongyan Ni, He Zhang, Xianqun Fan
Autophagy plays an adaptive role in cell survival, development, differentiation and intracellular homeostasis. Autophagy is recognized as a 'self-cannibalizing' process that is active during stresses such as starvation, chemotherapy, infection, ageing, and oxygen shortage to protect organisms from various irritants and to regenerate materials and energy. However, autophagy can also lead to a form of programmed cell death distinct from apoptosis. Components of the autophagic pathway are constitutively expressed at a high level in the eye, including in the cornea, lens, retina, and orbit...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27843433/successful-treatment-of-lasiodiplodia-theobromae-keratitis-assessing-the-role-of-voriconazole
#10
Stephen Tak-Lun Li, Evan Po-Fat Yiu, Angela Hiu-Yan Wong, John Chun-Ting Yeung, Lester Wang-Hon Yu
PURPOSE: The aim of this paper is to report the successful management of the first case of Lasiodiplodia theobromae keratitis in Hong Kong. METHODS: We conducted a case report. RESULTS: A 43-year-old Chinese male with a history of diabetes developed left eye keratitis after a trauma during tree felling. Fungal keratitis was diagnosed using a confocal microscope on day 1, and L. theobromae was confirmed from the culture. He was given oral voriconazole, topical natamycin, and topical and intracameral amphotericin B...
September 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/27777123/descemet-membrane-adhesion-strength-is-greater-in-diabetics-with-advanced-disease-compared-to-healthy-donor-corneas
#11
Chaid Schwarz, Benjamin T Aldrich, Kimberlee A Burckart, Gregory A Schmidt, M Bridget Zimmerman, Cynthia R Reed, Mark A Greiner, Edward A Sander
Descemet membrane endothelial keratoplasty (DMEK) is an increasingly popular surgical procedure for treating ocular diseases that require a corneal transplant. Previous studies have found that tissue tearing during surgical preparation is more likely elevated in eyes from donors with a history of diabetes mellitus. To quantify these potential differences, we established an experimental technique for quantifying the force required to separate the endothelium-Descemet membrane complex (EDM) from stroma in human donor corneal tissue, and we assessed differences in adhesion strength between diabetic and non-diabetic donor corneas...
October 21, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27774316/effect-of-treatment-with-salsalate-menhaden-oil-combination-of-salsalate-and-menhaden-oil-or-resolvin-d1-of-c57bl-6j-type-1-diabetic-mouse-on-neuropathic-endpoints
#12
Matthew S Yorek, Lawrence J Coppey, Hanna Shevalye, Alexander Obrosov, Randy H Kardon, Mark A Yorek
Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, menhaden oil, the combination of salsalate and menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad...
2016: Journal of Nutrition and Metabolism
https://www.readbyqxmd.com/read/27771327/in%C3%A2-vivo-confocal-microscopy-of-corneal-nerves-in-health-and-disease
#13
REVIEW
Andrea Cruzat, Yureeda Qazi, Pedram Hamrah
In vivo confocal microscopy (IVCM) is becoming an indispensable tool for studying corneal physiology and disease. Enabling the dissection of corneal architecture at a cellular level, this technique offers fast and noninvasive in vivo imaging of the cornea with images comparable to those of ex vivo histochemical techniques. Corneal nerves bear substantial relevance to clinicians and scientists alike, given their pivotal roles in regulation of corneal sensation, maintenance of epithelial integrity, as well as proliferation and promotion of wound healing...
January 2017: Ocular Surface
https://www.readbyqxmd.com/read/27760856/high-throughput-screens-to-discover-small-molecule-modulators-of-ryanodine-receptor-calcium-release-channels
#14
Robyn T Rebbeck, Maram M Essawy, Florentin R Nitu, Benjamin D Grant, Gregory D Gillispie, David D Thomas, Donald M Bers, Razvan L Cornea
Using time-resolved fluorescence resonance energy transfer (FRET), we have developed and validated the first high-throughput screening (HTS) method to discover compounds that modulate an intracellular Ca(2+) channel, the ryanodine receptor (RyR), for therapeutic applications. Intracellular Ca(2+) regulation is critical for striated muscle function, and RyR is a central player. At resting [Ca(2+)], an increased propensity of channel opening due to RyR dysregulation is associated with severe cardiac and skeletal myopathies, diabetes, and neurological disorders...
October 19, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27749499/assessment-of-corneal-backward-light-scattering-in-diabetic-patients
#15
Pelin Özyol, Erhan Özyol
OBJECTIVES: To analyze corneal backward light scattering differences in patients with type 2 diabetes mellitus. METHODS: We enrolled 43 eyes from 43 diabetic patients and 40 eyes from 40 healthy controls. Corneal backward light scattering was evaluated using densitometry measurements from different corneal layers and zones obtained using Scheimpflug tomography (PentacamHR). RESULTS: When densitometry values were divided by depth, anterior layer of diabetic corneas displayed significantly higher corneal backward light scattering values than controls (32...
October 3, 2016: Eye & Contact Lens
https://www.readbyqxmd.com/read/27742548/complete-metabolome-and-lipidome-analysis-reveals-novel-biomarkers-in-the-human-diabetic-corneal-stroma
#16
Shrestha Priyadarsini, Tina B McKay, Akhee Sarker-Nag, Jeremy Allegood, Charles Chalfant, Jian-Xing Ma, Dimitrios Karamichos
Prolonged hyperglycemia during diabetes mellitus can cause severe ophthalmic complications affecting both the anterior and posterior ocular segments leading to impaired vision or blindness. Diabetes-induced corneal pathologies are associated with decreased wound healing capacity, corneal edema, and altered epithelial basement membrane. The mechanism by which diabetes modulates structure and function within the corneal stroma are unknown. In our study, we characterized the effects of diabetes on extracellular matrix, lipid transport, and cellular metabolism by defining the entire metabolome and lipidome of Type 1 and Type 2 human diabetic corneal stroma...
October 11, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27660697/in-vivo-corneal-confocal-microscopy-in-diabetes-where-we-are-and-where-we-can-get
#17
REVIEW
Ernesto Maddaloni, Francesco Sabatino
In vivo corneal confocal microscopy (IVCCM) is a novel, reproducible, easy and noninvasive technique that allows the study of the different layers of the cornea at a cellular level. As cornea is the most innervated organ of human body, several studies investigated the use of corneal confocal microscopy to detect diabetic neuropathies, which are invalidating and deadly complications of diabetes mellitus. Corneal nerve innervation has been shown impaired in subjects with diabetes and a close association between damages of peripheral nerves due to the diabetes and alterations in corneal sub-basal nerve plexus detected by IVCCM has been widely demonstrated...
September 15, 2016: World Journal of Diabetes
https://www.readbyqxmd.com/read/27659544/a-randomized-multicenter-clinical-trial-of-ultrathin-descemet-stripping-automated-endothelial-keratoplasty-dsaek-versus-dsaek
#18
Mor M Dickman, Pieter J Kruit, Lies Remeijer, Jeroen van Rooij, Allegonda Van der Lelij, Robert H J Wijdh, Frank J H M van den Biggelaar, Tos T J M Berendschot, Rudy M M A Nuijts
OBJECTIVE: To compare visual acuity, refraction, endothelial cell density (ECD), and complications after Descemet stripping automated endothelial keratoplasty (DSAEK) and ultrathin DSAEK (UT-DSAEK). DESIGN: A multicenter, prospective, double-masked, randomized, controlled clinical trial. PARTICIPANTS: From 66 patients with irreversible corneal endothelial dysfunction dues to Fuchs' dystrophy who enrolled from 4 tertiary medical centers in the Netherlands, 66 eyes were studied...
September 19, 2016: Ophthalmology
https://www.readbyqxmd.com/read/27623389/corneal-thickness-differences-between-type-2-diabetes-and-non-diabetes-subjects-during-preoperative-laser-surgery-examination
#19
Juan A Sanchis-Gimeno, Luis Alonso, Mohammed Rahhal, Markus Bastir, Marcelino Perez-Bermejo, Lurdes Belda-Salmeron
AIMS: To evaluate the differences in corneal thickness between type 2 diabetes subjects with HbA1c under 7.0% and non-diabetes subjects during their preoperative laser surgery examinations. METHODS: The mean of five consecutive corneal thickness measurements at the central and mid-peripheral cornea was obtained by means of noncontact scanning-slit corneal topography (Orbscan Topography System II; Orbscan, Inc., Salt Lake City, UT, USA) in 35 myopic non-insulin dependent type 2 diabetes subjects (17 males and 18 females) and 48 healthy myopic controls (23 males and 25 females)...
January 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/27618414/diabetes-impairs-the-aldehyde-detoxifying-capacity-of-the-retina
#20
Rosemary E McDowell, Mary K McGahon, Josy Augustine, Mei Chen, J Graham McGeown, Tim M Curtis
PURPOSE: We studied whether the accumulation of advanced lipoxidation end-products (ALEs) in the diabetic retina is linked to the impairment of lipid aldehyde detoxification mechanisms. METHODS: Retinas were collected from nondiabetic and diabetic rats and processed for conventional and quantitative RT-PCR (qRT-PCR), Western blotting, immunohistochemistry, and aldehyde dehydrogenase (ALDH) activity assays. The effect of the ALDH1a1 inhibitor, NCT-501, on ALE accumulation and cell viability in cultured Müller glia also was investigated...
September 1, 2016: Investigative Ophthalmology & Visual Science
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