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Descemet's membrane

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https://www.readbyqxmd.com/read/28525820/sensing-inhomogeneous-mechanical-properties-of-human-corneal-descemet-s-membrane-with-afm-nano-indentation
#1
Rosa Di Mundo, Giuseppina Recchia, Mohit Parekh, Alessandro Ruzza, Stefano Ferrari, Giuseppe Carbone
The paper describes a highly space-resolved characterization of the surface mechanical properties of the posterior human corneal layer (Descemet's membrane). This has been accomplished with Atomic Force Microscopy (AFM) nano-indentation by using a probe with a sharp tip geometry. Results indicate that the contact with this biological tissue in liquid occurs with no (or very low) adhesion. More importantly, under the same operating conditions, a broad distribution of penetration depth can be measured on different x-y positions of the tissue surface, indicating a high inhomogeneity of surface stiffness, not yet clearly reported in the literature...
May 10, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28509328/anatomy-based-dmek-wetlab-in-homburg-saar-novel-aspects-of-donor-preparation-and-host-maneuvers-to-teach-descemet-membrane-endothelial-keratoplasty
#2
B Seitz, L Daas, M Bischoff-Jung, N Szentmáry, S Suffo, M El-Husseiny, A Viestenz, G Milioti
INTRODUCTION: Use of Descemet Membrane Endothelial Keratoplasty (DMEK) has been limited because of problems with donor preparation, i.e. tearing of the Descemet membrane and difficulties in unfolding the Endothelium-Descemet-Membrane-Layer (EDML) in the anterior chamber (AC). The purpose of this work was to describe a novel approach to teaching anatomy-based donor and recipient preparation in a DMEK-Wetlab. MATERIAL AND METHODS: We teach successful mono-manual donor preparation of human corneas in organ culture not suitable for transplantation, including peripheral markers for orientation...
May 16, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28506643/epithelial-basement-membrane-injury-and-regeneration-modulates-corneal-fibrosis-after-pseudomonas-corneal-ulcers-in-rabbits
#3
Gustavo K Marino, Marcony R Santhiago, Santhanam Abirami, Luciana L Dibbin, Thangavadivel Shanmugapriya, Carla S Medeiros, Karthikeyan Bose, Kwai Ping Tam, Steven E Wilson
The purpose of this study was to investigate whether myofibroblast-related fibrosis (scarring) after microbial keratitis was modulated by the epithelial basement membrane (EBM) injury and regeneration. Rabbits were infected with Pseudomonas aeruginosa after epithelial scrape injury and the resultant severe keratitis was treated with topical tobramycin. Corneas were analyzed from one to four months after keratitis with slit lamp photos, immunohistochemistry for alpha-smooth muscle actin (α-SMA) and monocyte lineage marker CD11b, and transmission electron microscopy...
May 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28489792/eye-bank-prepared-versus-surgeon-cut-endothelial-graft-tissue-for-descemet-membrane-endothelial-keratoplasty-an-observational-study
#4
Marie Regnier, Céline Auxenfans, Delphine Maucort-Boulch, Anne-Sophie Marty, Odile Damour, Carole Burillon, Viridiana Kocaba
The purpose of this article is to examine outcomes of Descemet membrane endothelial keratoplasty (DMEK) performed with cornea bank (CB) prestripped tissue and surgeon stripped tissue (SST).This retrospective study examined subjects who underwent DMEK with CB or surgeon prepared tissue for Fuchs endothelial corneal dystrophy. Best-corrected visual acuity (BCVA), corneal thickness, endothelial cell count (ECC), and complications were examined before and throughout a 6-month postoperative period.Eleven CB and 22 SST subjects were included...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28486726/management-of-interface-fluid-syndrome-after-lasik-by-descemet-membrane-endothelial-keratoplasty-in-a-patient-with-fuchs-corneal-endothelial-dystrophy
#5
Mehdi Shajari, Parviz Rafiezadeh, Ivana Pavlovic, Kate Barbara Kubiak, Thomas Kohnen, Ingo Schmack
PURPOSE: To report a case of interface fluid syndrome after LASIK in a patient with Fuchs' corneal endothelial dystrophy treated with Descemet membrane endothelial keratoplasty (DMEK). METHODS: Case report. RESULTS: A 74-year-old patient presented with interface fluid syndrome and was treated with DMEK. Its complications in the form of partial graft detachment and cystoid macular edema were successfully managed by rebubbling and topical treatment, respectively...
May 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28483495/intraocular-lens-calcifications-after-triple-descemet-membrane-endothelial-keratoplasty-dmek
#6
Silvia Schrittenlocher, Marius Penier, Friederike Schaub, Felix Bock, Claus Cursiefen, Björn Bachmann
PURPOSE: To identify incidence of and risk factors for calcifications of intraocular lenses (IOLs) after Descemet membrane endothelial keratoplasty (DMEK). DESIGN: Retrospective cohort study. METHODS: Retrospective review of charts and slit-lamp images of 564 consecutive patients from the prospective Cologne DMEK database who underwent DMEK in pseudophacic eyes or DMEK in combination with cataract surgery (triple-DMEK) between 09/2013 and 10/2015 at the Department of Ophthalmology, University of Cologne...
May 5, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28481010/dynamics-of-big-bubble-formation-in-deep-anterior-lamellar-keratoplasty-by-the-big-bubble-technique-in%C3%A2-vitro-studies
#7
Harminder S Dua, Lana A Faraj, Mohamed B Kenawy, Saief AlTaan, Mohamed S Elalfy, Tarek Katamish, Dalia G Said
PURPOSE: To examine the movement of air injected in the cornea in simulated deep anterior lamellar keratoplasty (DALK), from the needle tip to the formation of different types of big bubbles (BB) and to ascertain how air travels through the stroma and pre-Descemet's layer [Dua's layer (PDL)] to create a type-1 (air between PDL and deep stroma) and type-2BB (air between PDL and Descemet's membrane). METHODS: Air was injected in 57 sclerocorneal discs and the passage recorded...
May 8, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28479036/-descemet-s-membrane-endothelial-keratoplasty-dmek-analysis-of-a-variation-in-descemet-s-endothelial-graft-preparation
#8
C Benoist D'azy, C Benoist D'azy, E Gabison, V Sapin, C Bosc, B Pereira, F Chiambaretta
INTRODUCTION: Descemet's membrane endothelial keratoplasty (DMEK) can replace just the corneal endothelium and respect the natural corneal anatomy. Currently, the technique of endothelial graft preparation remains manual and non-standardized. PURPOSE: To report anatomic and functional results after DMEK, and compare two techniques of graft preparation. METHODS: Single-center retrospective study, including 64 eyes of 64 patients undergoing DMEK, from September 2014 to February 2016 at Clermont-Ferrand University Medical Center...
May 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28473428/to-show-the-efficacy-of-compressive-sutures-alone-in-the-management-of-acute-hydrops-in-a-keratoconus-patient
#9
Praveen Subudhi, Zahiruddin Khan, B Nageswar Rao Subudhi, Silla Sitaram
A 17-year-old boy presented with sudden loss of vision in the left eye (OS) for 3 days. He was diagnosed with acute hydrops following keratoconic progression in OS. The patient was initially started on topical medical therapy, including steroids and hypertonic eye drops; showing no signs of resolution. Hence, the patient was planned for full-thickness compressive corneal sutures. Four sutures were placed along the central oedematous area covering the area of ruptured Descemet's membrane. Signs of resolution were noticed by 1st week and there was complete resolution of oedema by 3rd post-op week...
May 4, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28456826/comparison-of-descemet-stripping-under-continuous-air-flow-manual-air-injection-and-balanced-salt-solution-for-dmek-a-pilot-study
#10
I E Gabbay, I Bahar, Y Nahum, E Livny
BACKGROUND: Descemet's membrane endothelial keratoplasty (DMEK) involves removal of the recipient's Descemet membrane (DM) prior to transplanting the donor's DM. When using balanced salt solution (BSS) or ophthalmic viscosurgical devices (OVDs), visualization of the host's DM during its stripping may be inadequate and may result in Descemet remnants and could lead to sub-optimal surgical results. Previous articles described excellent visualization when utilizing air injection but this requires repeated air injection into the anterior chamber (AC)...
April 29, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28440248/descemet-stripping-automated-endothelial-keratoplasty
#11
Namrata Sharma, Prafulla K Maharana, Shipra Singhi, Neelima Aron, Mukesh Patil
Endothelial keratoplasty is at present the gold standard for surgical treatment of corneal endothelial pathologies not associated with significant corneal scar. Tremendous progress has been made in recent years in improving the technology of endothelial keratoplasty techniques, such as descemet stripping automated endothelial keratoplasty (DSAEK) and descemet membrane endothelial keratoplasty. In this review, we discuss the current techniques and outcomes of DSAEK.
March 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28437276/clinical-outcome-of-rebubbling-for-graft-detachment-after-descemet-membrane-endothelial-keratoplasty
#12
Nadine Gerber-Hollbach, Lamis Baydoun, Ester Fernández López, Laurence E Frank, Isabel Dapena, Vasilios S Liarakos, Sontje-Chiao Schaal, Lisanne Ham, Silke Oellerich, Gerrit R J Melles
PURPOSE: To assess the clinical outcome after successful rebubbling procedures for visually significant graft detachment after Descemet membrane endothelial keratoplasty (DMEK). METHODS: From a total of 760 consecutive DMEK surgeries, 41 eyes required rebubbling. Of those, 33 eyes of 31 patients were successful and were included in our retrospective outcome analysis study. Main outcome measures were compared with those of matched controls with uneventful primary DMEK (attached DMEK grafts without rebubbling)...
April 21, 2017: Cornea
https://www.readbyqxmd.com/read/28437275/descemet-membrane-endothelial-keratoplasty-after-failed-descemet-stripping-without-endothelial-keratoplasty
#13
Rohini Rao, Durga S Borkar, Kathryn A Colby, Peter B Veldman
PURPOSE: To describe the clinical course, surgical experience, and postoperative outcomes of 3 patients with Fuchs endothelial dystrophy who underwent Descemet membrane endothelial keratoplasty (DMEK) after failed Descemet stripping without endothelial keratoplasty. METHODS: Three patients who underwent DMEK for management of persistent corneal edema after deliberate Descemet stripping in the setting of Fuchs endothelial dystrophy were identified. Patients were examined at day 1, week 1, and months 1, 3, and 6 after DMEK...
April 21, 2017: Cornea
https://www.readbyqxmd.com/read/28430178/endothelial-keratoplasty-is-descemet-membrane-endothelial-keratoplasty-the-holy-grail-of-lamellar-surgery-no
#14
M Tsatsos, V S Liarakos, C MacGregor, I Athanasiadis, E T Detorakis, M M Moschos, P Hossain, D F Anderson
No abstract text is available yet for this article.
April 21, 2017: Eye
https://www.readbyqxmd.com/read/28421398/supradescemetic-voriconazole-injection-for-candida-parapsilosis-keratitis
#15
Nikolaos Mamas, Konstantinos Andreanos, Klio Chatzistefanou, Petros Petrou, Dimitrios Brouzas, George Kymionis, Chrysanthi Koutsandrea, Konstantinos Droutsas
PURPOSE: To describe a technique for intracorneal application of voriconazole into the supradescemetic space in a case of deep recalcitrant Candida parapsilosis keratitis of a penetrating cornea graft. METHODS: A deep intracorneal incision reaching the center of the corneal infiltrate was created with a 20-gauge MVR blade. Then, a 27-gauge hydrodissection cannula was inserted deep into the corneal pocket, and 0.1 ml of voriconazole 0.5 mg/ml was injected until a bullous detachment of Descemet membrane (DM) covered 1/3 of the graft's area...
April 18, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28417337/descemet-stripping-automated-endothelial-keratoplasty-versus-descemet-membrane-endothelial-keratoplasty-a-meta-analysis
#16
REVIEW
Leru Zhu, Yi Zha, Jianqiu Cai, Yanling Zhang
PURPOSE: To evaluate the efficacy and safety of descemet stripping automated endothelial keratoplasty (DSAEK) compared with descemet membrane endothelial keratoplasty (DMEK). METHODS: Databases including PubMed, EMBASE, and Cochrane Library were searched to find studies that compared DSAEK and DMEK outcomes. Efficacy parameters were the postoperative best-corrected visual acuity (BCVA) and spherical equivalent (SE). Safety parameters were postoperative endothelial cell loss (ECL), air injection (rebubbling), graft failure, graft rejection, and high intraocular pressure (IOP)...
April 17, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28416495/ultrastructural-study-of-peripheral-and-central-stroma-of-keratoconus-cornea
#17
Aljoharah Alkanaan, Robert Barsotti, Omar Kirat, Turki Almubrad, Adnan Khan, Saeed Akhtar
PURPOSE: Assess the lamellar organisation of the peripheral and central stroma of the keratoconus (KC) and normal cornea. METHODS: Five normal and three KC corneas were fixed in 2.5% glutaraldehyde and processed for electron microscopy. The ultrathin sections were observed under JEOL 1400 TEM, and digital images were taken with a bottom-mounted 11-megapixel Quamisa camera, using the iTEM software. Measurements of the lamellae were carried out using the iTEM software...
April 17, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28410548/two-year-clinical-outcome-of-500-consecutive-cases-undergoing-descemet-membrane-endothelial-keratoplasty
#18
Jorge Peraza-Nieves, Lamis Baydoun, Isabel Dapena, Abbas Ilyas, Laurence E Frank, Salvatore Luceri, Lisanne Ham, Silke Oellerich, Gerrit R J Melles
PURPOSE: To evaluate the clinical outcome of 500 consecutive cases up to 2 years after Descemet membrane endothelial keratoplasty (DMEK) and to assess which parameters may have influenced the clinical outcome. METHODS: From a group of 500 eyes (393 patients), which underwent DMEK for Fuchs endothelial corneal dystrophy (FECD), bullous keratopathy, failed corneal transplants and other indications, clinical outcomes [best-corrected visual acuity (BCVA), central endothelial cell density (ECD), and central corneal thickness] were evaluated before, and at 6, 12, and 24 months after DMEK and postoperative complications were documented...
June 2017: Cornea
https://www.readbyqxmd.com/read/28410359/intraoperative-s-stamp-enabled-rescue-of-3-inverted-descemet-membrane-endothelial-keratoplasty-grafts
#19
Peter B Veldman, Zachary M Mayko, Michael D Straiko, Mark A Terry
PURPOSE: To report the clinical outcomes of 3 Descemet membrane endothelial keratoplasty (DMEK) grafts, in which an upside down DMEK graft orientation was discovered after SF6 gas insufflation of the anterior chamber and the orientation was then corrected intraoperatively. METHODS: A total of 371 consecutive DMEK cases using an S-stamp were analyzed and found to include 3 cases in which the DMEK graft was initially elevated with SF6 gas in an upside down orientation, as demonstrated intraoperatively by the S-stamp...
June 2017: Cornea
https://www.readbyqxmd.com/read/28405478/comparison-of-outcomes-in-patients-who-underwent-deep-anterior-lamellar-keratoplasty-and-those-converted-to-penetrating-keratoplasty
#20
Yusuf Koçluk, Emine Alyamaç Sukgen, Ayşe Burcu
OBJECTIVES: To compare clinical outcomes of cases who underwent deep anterior lamellar keratoplasty (DALK) and cases who were converted to penetrating keratoplasty (PKP) from DALK surgery. MATERIALS AND METHODS: The records of 54 patients for whom DALK surgery was planned and were operated for different diagnoses between March 2013 and June 2015 were retrospectively analyzed. Patients were divided into two groups: group 1 (PKP group) consisted of 23 cases who were converted to PKP due to Descemet's membrane perforation at any stage of surgery; group 2 (DALK group) consisted of 31 patients whose surgery could be completed as DALK...
April 2017: Turkish Journal of Ophthalmology
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