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https://www.readbyqxmd.com/read/28918606/kinetic-coupling-of-phosphate-release-force-generation-and-rate-limiting-steps-in-the-cross-bridge-cycle
#1
Robert Stehle, Chiara Tesi
A basic goal in muscle research is to understand how the cyclic ATPase activity of cross-bridges is converted into mechanical force. A direct approach to study the chemo-mechanical coupling between Pi release and the force-generating step is provided by the kinetics of force response induced by a rapid change in [Pi]. Classical studies on fibres using caged-Pi discovered that rapid increases in [Pi] induce fast force decays dependent on final [Pi] whose kinetics were interpreted to probe a fast force-generating step prior to Pi release...
September 16, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28918555/prognostic-factors-for-mortality-in-middle-aged-and-older-hemodialysis-patients-a-5-year-observational-study
#2
Kojiro Nagai, Motokazu Matsuura, Kenji Tsuchida, Hiro-Omi Kanayama, Toshio Doi, Jun Minakuchi
Clinical guidelines for hemodialysis therapy have been described in an evidence-based manner with most evidence from randomized control trials or retrospective studies in which all generations of the hemodialysis patients were enrolled. Therefore, the question still remains whether these guidelines can be applied to increasing older patients. This study is an observational study, including 735 patients who received maintenance hemodialysis in April 2006. At baseline, the participants' age was 62.1 ± 12...
September 16, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/28918503/coq0-induced-mitochondrial-ptp-opening-triggers-apoptosis-via-ros-mediated-vdac1-upregulation-in-hl-60-leukemia-cells-and-suppresses-tumor-growth-in-athymic-nude-mice-xenografted-nude-mice
#3
You-Cheng Hseu, Varadharajan Thiyagarajan, Ting-Tsz Ou, Hsin-Ling Yang
Coenzyme Q (CoQ) analogs with variable numbers of isoprenoid units have been demonstrated as anticancer and antioxidant/pro-oxidant molecules. This study examined the in vitro and in vivo antitumor and apoptosis activities of CoQ0 (2,3-dimethoxy-5-methyl-1,4-benzoquinone, zero isoprenoid side-chains) through upregulation of the Voltage-dependent anion channel 1 (VDAC1) signaling pathway on human promyelocytic leukemia. CoQ0 (0-40 μg/mL) treatment significantly reduced HL-60 cell viability, and up-regulated mitochondrial VDAC1 expression...
September 16, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28918434/improved-dna-purification-with-quality-assurance-for-evaluation-of-the-microbial-genetic-content-of-constructed-wetlands
#4
Wenda Huang, Jingjing Guo, Ran Tao, Ying Man, Yunv Dai, Yang Yang
Efficient isolation of target DNA is a crucial first step of DNA-based metagenomic analyses of environmental samples. Insufficient quantity and purity of DNA isolated using commercial kits result in missing genetic information, especially for large-diameter substrates in constructed wetlands (CWs). Here, we addressed this problem by devising a cost-effective calcium chloride lysozyme-sodium dodecyl sulfate (SDS) method (CCLS), with key improvements in the steps of humic acid removal and cell lysis. The buffer comprising Tris, EDTA, Na2O2P7 and PVPP (TENP), and skim milk, could reduce adsorption between microorganisms and substrates, and calcium chloride precipitated and removed over 94% of humic acid...
September 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28918394/apolipoprotein-l1-confers-ph-switchable-ion-permeability-to-phospholipid-vesicles
#5
Jonathan Bruno, Nicola Pozzi, Jonathan Oliva, John C Edwards
Apolipoprotein L1 (ApoL1) is a human serum protein conferring resistance to African trypanosomes, and certain ApoL1 variants increase susceptibility to some progressive kidney diseases. ApoL1 has been hypothesized to function like a pore-forming colicin and been reported to have permeability effects on both intracellular and plasma membranes. Here, to gain insight into how ApoL1 may function in vivo, we used vesicle-based ion-permeability, direct membrane-association, and intrinsic fluorescence to study the activities of purified recombinant ApoL1...
September 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28918145/short-communication-relationship-between-methods-for-measurement-of-serum-electrolytes-and-the-relationship-between-ionized-and-total-calcium-and-neutrophil-oxidative-burst-activity-in-early-postpartum-dairy-cows
#6
B M Leno, E M Martens, M J B Felippe, K P Zanzalari, J C Lawrence, T R Overton
The objectives of this study were to (1) compare a test for serum measurement of total Ca (tCa), Mg, and P (VetTest Chemistry Analyzer, IDEXX Laboratories Inc., Westbrook, ME) to reference methods (spectrophotometric assays on a Beckman Coulter 640e automated clinical chemistry analyzer; Beckman Coulter, Brea, CA), (2) determine the relationship between ionized Ca (iCa) and reference method tCa in the immediate postpartum period, and (3) assess the relative value of these blood Ca indices as predictors of neutrophil oxidative burst activity...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28918139/comparison-of-milk-protein-composition-and-rennet-coagulation-properties-in-native-swedish-dairy-cow-breeds-and-high-yielding-swedish-red-cows
#7
Nina A Poulsen, Maria Glantz, Anette K Rosengaard, Marie Paulsson, Lotte B Larsen
Recent studies have reported a very high frequency of noncoagulating milk in Swedish Red cows. The underlying factors are not fully understood. In this study, we explored rennet-induced coagulation properties and relative protein profiles in milk from native Swedish Mountain and Swedish Red Polled cows and compared them with a subset of noncoagulating (NC) and well-coagulating (WC) milk samples from modern Swedish Red cows. The native breeds displayed a very low prevalence of NC milk and superior milk coagulation properties compared with Swedish Red cows...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28918134/crystallization-and-demineralization-phenomena-in-washed-rind-cheese
#8
Gil F Tansman, Paul S Kindstedt, John M Hughes
This report documents an observational study of a high-moisture washed-rind cheese. Three batches of cheese were sampled on a weekly basis for 6 wk and again at wk 10. Center, under-rind, rind, and smear samples were tested for pH, moisture, and selected mineral elements. Powder x-ray diffractometry and petrographic microscopy were applied to identify and image the crystal phases. The pH of the rind increased by over 2 pH units by wk 10. The pH of the under-rind increased but remained below the rind pH, whereas the center pH decreased for most of aging and only began to rise after wk 5...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28918079/clogging-of-an-alpine-streambed-by-silt-sized-particles-insights-from-laboratory-and-field-experiments
#9
Jasmin Fetzer, Markus Holzner, Michael Plötze, Gerhard Furrer
Clogging of streambeds by suspended particles (SP) can cause environmental problems, as it can negatively influence, e.g., habitats for macrozoobenthos, fish reproduction and groundwater recharge. This especially applies in the case of silt-sized SP. Until now, most research has dealt with coarse SP and was carried out in laboratory systems. The aims of this study are to examine (1) whether physical clogging by silt-sized SP exhibits the same dynamics and patterns as by sand-sized SP, and (2) the comparability of results between laboratory and field experiments...
September 7, 2017: Water Research
https://www.readbyqxmd.com/read/28918025/effect-of-calcium-carbonate-encapsulation-on-the-activity-of-orally-administered-cpg-oligonucleotides
#10
Neslihan Kayraklioglu, Julia Scheiermann, W Gregory Alvord, Dennis M Klinman
Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) stimulate immune cells via Toll-like receptor 9 (TLR9). Because oligodeoxynucleotides (ODNs) are susceptible to gastric degradation, clinical trials designed to evaluate their therapeutic utility have relied solely on parenteral routes of administration. A strategy to improve the activity of orally delivered ODNs by reducing their susceptibility to gastrointestinal (GI) digestion via encapsulation in calcium carbonate nanoparticles (ODNcaps) was recently described...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917911/injectable-glycosaminoglycan-protein-nano-complex-in-semi-interpenetrating-networks-a-biphasic-hydrogel-for-hyaline-cartilage-regeneration
#11
Janani Radhakrishnan, Anuradha Subramanian, Swaminathan Sethuraman
Articular hyaline cartilage regeneration remains challenging due to its less intrinsic reparability. The study develops injectable biphasic semi-interpenetrating polymer networks (SIPN) hydrogel impregnated with chondroitin sulfate (ChS) nanoparticles for functional cartilage restoration. ChS loaded zein nanoparticles (∼150nm) prepared by polyelectrolyte-protein complexation were interspersed into injectable SIPNs developed by blending alginate with poly(vinyl alcohol) and calcium crosslinking. The hydrogel exhibited interconnected porous microstructure (39...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917880/intercalated-chitosan-hydroxyapatite-nanocomposites-promising-materials-for-bone-tissue-engineering-applications
#12
Muhammad Anwaar Nazeer, Emel Yilgör, Iskender Yilgör
Preparation and characterization of chitosan/hydroxyapatite (CS/HA) nanocomposites displaying an intercalated structure is reported. Hydroxyapatite was synthesized through sol-gel process. Formic acid was introduced as a new solvent to obtain stable dispersions of nano-sized HA particles in polymer solution. CS/HA dispersions with HA contents of 5, 10 and 20% by weight were prepared. Self-assembling of HA nanoparticles during the drying of the solvent cast films led to the formation of homogeneous CS/HA nanocomposites...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917876/preparation-of-chitosan-hydroxyapatite-composite-mono-fiber-using-coagulation-method-and-their-mechanical-properties
#13
Takuma Okada, Yuta Nobunaga, Toshiisa Konishi, Tomohiko Yoshioka, Satoshi Hayakawa, Maria Ascensão Lopes, Toshiki Miyazaki, Yuki Shirosaki
Autograft has been carried out for anterior cruciate ligament (ACL) reconstruction surgery. However, it has negative aspect because patients lose their healthy ligaments from other part. We focus on a chitosan-hydroxyapatite (HAp) composite fiber as a scaffold of ligament regeneration. Chitosan- HAp composite fiber was made by using coagulation method. Chitosan-NaH2PO4 solution was coagulated with coagulation bath including calcium ion to get the mono-fiber and then treated with sodium hydroxide solution to form HAp in fiber matrix...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917869/high-aspect-ratio-phospho-calcified-rock-candy-like-cellulose-nanowhiskers-of-wastepaper-applicable-in-osteogenic-differentiation-of-hmscs
#14
Mohsen Shahrousvand, Fatemeh Ahmadi Tabar, Ehsan Shahrousvand, Amir Babaei, Mohammad Mahdi Hasani-Sadrabadi, Gity Mir Mohamad Sadeghi, Hafez Jafari, Ali Salimi
The aim of this study was to prepare cellulose nanowhiskers (CNWs) from wastepaper powder (WPP), as an environmentally friendly approach for obtaining the source material, which is a highly available and low-cost precursor for cellulose nanomaterial processing. Acid hydrolysis and calcification treatments were employed for extraction of CNWs and preparation of novel phospho-calcified cellulose nanowhiskers (PCCNWs). CNWs and PCCNWs were analyzed through optical microscopy (OM), scanning electron microscopy (SEM), Fourier-transformed infrared spectra (FTIR) and X-ray diffraction analysis (XRD)...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917714/mitochondria-in-the-nervous-system-from-health-to-disease-part-i
#15
EDITORIAL
Brian M Polster, Maria Teresa Carrì, Philip M Beart
In Part I of this Special Issue on "Mitochondria in the Nervous System: From Health to Disease", the editors bring together contributions from experts in brain mitochondrial research to provide an up-to-date overview of mitochondrial functioning in physiology and pathology. The issue provides cutting edge reviews on classical areas of mitochondrial biology that include energy substrate utilization, calcium handling, mitochondria-endoplasmic reticulum communication, and cell death regulation. Additional reviews and original research articles touch upon key mitochondrial defects seen across multiple neurodegenerative conditions, including fragmentation, loss of respiratory capacity, calcium overload, elevated reactive oxygen species generation, perturbed NAD(+) metabolism, altered protein acetylation, and compromised mitophagy...
September 13, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28917674/prognostic-value-of-risk-factors-calcium%C3%A2-score-coronary-cta-myocardial%C3%A2-perfusion-imaging-and-invasive%C3%A2-coronary-angiography-in-kidney%C3%A2-transplantation-candidates
#16
Simon Winther, My Svensson, Hanne Skou Jørgensen, Laust Dupont Rasmussen, Niels Ramsing Holm, Lars Christian Gormsen, Kirsten Bouchelouche, Hans Erik Bøtker, Per Ivarsen, Morten Bøttcher
OBJECTIVES: This study sought to perform a prospective head-to-head comparison of the predictive value of clinical risk factors and a variety of cardiac imaging modalities including coronary artery calcium score (CACS), coronary computed tomography angiography (CTA), single-photon emission computed tomography (SPECT), and invasive coronary angiography (ICA) on major adverse cardiac events (MACE) and all-cause mortality in kidney transplantation candidates. BACKGROUND: Current guidelines recommend screening for coronary artery disease in kidney transplantation candidates...
September 9, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28917577/direct-pulp-capping-with-calcium-hydroxide-mineral-trioxide-aggregate-and-biodentine-in%C3%A2-permanent-young-teeth-with-caries-a%C3%A2-randomized-clinical-trial
#17
Claudia Brizuela, Andrea Ormeño, Carolina Cabrera, Roxana Cabezas, Carolina Inostroza Silva, Valeria Ramírez, Montse Mercade
INTRODUCTION: Direct pulp capping treatment is intended to preserve pulp vitality, to avoid or retard root canal treatment, and, in cases with an open apex, to allow continued root development. Historically, calcium hydroxide (CH) was the gold standard material, but nowadays calcium silicate materials (CSMs) are displacing CH because of their high bioactivity, biocompatibility, sealing ability, and mechanical properties. However, more randomized clinical trials are needed to confirm the appropriateness of CSMs as replacement materials for CH in direct pulp capping procedures...
September 13, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28917262/determination-of-several-element-levels-in-hardaliye-beverages-using-flame-atomic-absorption-spectrometry-after-ultrasound-probe-extraction
#18
Dilek Bakircioglu, Nukte Topraksever, Yasemin Bakircioglu Kurtulus
In the present study, concentrations of calcium (Ca), copper (Cu), magnesium (Mg), manganese (Mn), sodium (Na), and zinc (Zn) in hardaliye samples produced in Turkey were determined by flame atomic absorption spectrometry after ultrasound probe extraction (UPE), microwave-assisted extraction (MAE), and wet extraction procedures. At present, there is limited work in the literature on UPE for the determination of trace elements in beverage samples. Our single-correlation analysis showed that the elements studied with the UPE method in hardaliye were strongly correlated with the MAE procedure...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28917183/visualisation-of-an-nspef-induced-calcium-wave-using-the-genetically-encoded-calcium-indicator-gcamp-in-u87-human-glioblastoma-cells
#19
Lynn Carr, Sylvia M Bardet, Delia Arnaud-Cormos, Philippe Leveque, Rodney P O'Connor
Cytosolic, synthetic chemical calcium indicators are typically used to visualise the rapid increase in intracellular calcium ion concentration that follows nanosecond pulsed electric field (nsPEF) application. This study looks at the application of genetically encoded calcium indicators (GECIs) to investigate the spatiotemporal nature of nsPEF-induced calcium signals using fluorescent live cell imaging. Calcium responses to 44kV/cm, 10ns pulses were observed in U87-MG cells expressing either a plasma membrane targeted GECI (GCaMP5-G), or one cytosolically expressed (GCaMP6-S), and compared to the response of cells loaded with cytosolic or plasma membrane targeted chemical calcium indicators...
September 8, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28917157/density-of-calcium-in-the-ascending-thoracic-aorta-and-risk-of-incident-cardiovascular-disease-events
#20
Isac C Thomas, Robyn L McClelland, Erin D Michos, Matthew A Allison, Nketi I Forbang, W T Longstreth, Wendy S Post, Nathan D Wong, Matthew J Budoff, Michael H Criqui
BACKGROUND AND AIMS: The volume and density of coronary artery calcium (CAC) both independently predict cardiovascular disease (CVD) beyond standard risk factors, with CAC density inversely associated with incident CVD after accounting for CAC volume. We tested the hypothesis that ascending thoracic aorta calcium (ATAC) volume and density predict incident CVD events independently of CAC. METHODS: The Multi-Ethnic Study of Atherosclerosis (MESA) is a prospective cohort study of participants without clinical CVD at baseline...
September 7, 2017: Atherosclerosis
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