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Ammonia

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https://www.readbyqxmd.com/read/28430414/dynamic-and-energetic-signatures-of-adenine-tracts-in-a-ra-dt-rna-dna-hybrid-and-in-homologous-rna-dna-rna-rna-and-dna-dna-double-helices
#1
Yuegao Huang, Irina M Russu
Nuclear magnetic resonance spectroscopy and proton exchange are being used to characterize the opening reactions of individual base pairs in the RNA-DNA hybrid 5'-rGCGAUAAAAAGGCC-3'/5'-dGGCCTTTTTATCGC-3'. The hybrid contains a central tract of five rA-dT base pairs. The rates and the equilibrium constant of the opening reaction for each base pair are determined from the dependence of the exchange rates of imino protons on ammonia concentration, at 10°C. The results are compared to those previously obtained by our laboratory for three homologous duplexes of the same base sequence (except for the appropriate T/U substitution), containing tracts of dA-rU, rA-rU or dA-dT base pairs...
April 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28429993/interactive-effects-of-protein-and-carbohydrates-on-production-of-microbial-metabolites-in-the-large-intestine-of-growing-pigs
#2
Marcin Taciak, Marcin Barszcz, Ewa Święch, Anna Tuśnio, Ilona Bachanek
The study aimed at determining the effect of protein type and indigestible carbohydrates on the concentration of microbial metabolites in the large intestine of pigs. The experiment involved 36 pigs (15 kg initial body weight) divided into six groups, fed cereal-based diets with highly digestible casein (CAS) or potato protein concentrate (PPC) of lower ileal digestibility. Each diet was supplemented with cellulose, raw potato starch or pectin. After 2 weeks of feeding, pigs were sacrificed and samples of caecal and ascending, transverse and descending colon digesta were collected for analyses of microbial metabolites...
June 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28429328/ammonia-production-from-amino-acid-based-biomass-like-sources-by-engineered-escherichia-coli
#3
Yosuke Mikami, Hisanari Yoneda, Yohei Tatsukami, Wataru Aoki, Mitsuyoshi Ueda
The demand for ammonia is expected to increase in the future because of its importance in agriculture, industry, and hydrogen transportation. Although the Haber-Bosch process is known as an effective way to produce ammonia, the process is energy-intensive. Thus, an environmentally friendly ammonia production process is desired. In this study, we aimed to produce ammonia from amino acids and amino acid-based biomass-like resources by modifying the metabolism of Escherichia coli. By engineering metabolic flux to promote ammonia production using the overexpression of the ketoisovalerate decarboxylase gene (kivd), derived from Lactococcus lactis, ammonia production from amino acids was 351 mg/L (36...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28428060/effects-of-chronic-ammonia-exposure-on-ammonia-metabolism-and-excretion-in-marine-medaka-oryzias-melastigma
#4
Na Gao, Limei Zhu, Zhiqiang Guo, Meisheng Yi, Li Zhang
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated the metabolism and excretion of ammonia in marine medaka Oryzias melastigma during chronic ammonia exposure. The fish were exposed to 0, 0.1, 0.3, 0.6, and 1.1 mmol l(-1) NH4Cl spiked seawater for 8 weeks. Exposure of 0.3-1.1 mmol l(-1) NH4Cl had deleterious effects on the fish, including significant reductions in growth, feed intake, and total protein content...
April 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28427668/synthesis-of-hydroxyapatite-nanorods-for-application-in-water-defluoridation-and-optimization-of-process-variables-advantage-of-ultrasonication-with-precipitation-method-over-conventional-method
#5
Dhiraj Mehta, Poonam Mondal, Virendra Kumar Saharan, Suja George
This research work presents the synthesis of hydroxyapatite (Hap) nanorods for defluoridation of drinking water by using both conventional (CM) and ultrasonication with precipitation (USPM) methods. Calcium nitrate was reacted with potassium phosphate in presence of ammonia for controlled pH to synthesize Hap nanorods, which was characterized using FTIR, XRD, SEM, TG-DTA, and TEM/EDS for determining its phase composition, structural and thermal decomposition behavior. When USPM method was used for synthesis, the yield of the Hap nanorods was improved from 83...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426987/a-review-on-nitrous-oxide-n2o-emissions-during-biological-nutrient-removal-from-municipal-wastewater-and-sludge-reject-water
#6
REVIEW
Theoni Maria Massara, Simos Malamis, Albert Guisasola, Juan Antonio Baeza, Constantinos Noutsopoulos, Evina Katsou
Nitrous oxide (N2O) is an important pollutant which is emitted during the biological nutrient removal (BNR) processes of wastewater treatment. Since it has a greenhouse effect which is 265 times higher than carbon dioxide, even relatively small amounts can result in a significant carbon footprint. Biological nitrogen (N) removal conventionally occurs with nitrification/denitrification, yet also through advanced processes such as nitritation/denitritation and completely autotrophic N-removal. The microbial pathways leading to the N2O emission include hydroxylamine oxidation and nitrifier denitrification, both activated by ammonia oxidizing bacteria, and heterotrophic denitrification...
April 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28426151/ammonothermal-synthesis-of-earth-abundant-nitride-semiconductors-znsin2-and-zngen2-and-dissolution-monitoring-by-in-situ-x-ray-imaging
#7
Jonas Häusler, Saskia Schimmel, Peter Wellmann, Wolfgang Schnick
In this contribution, first synthesis of semiconducting ZnSiN2 and ZnGeN2 from solution is presented with supercritical ammonia as solvent and KNH2 as ammonobasic mineralizer. The reactions were conducted in custom-built high-pressure autoclaves made of nickel-based superalloy. The nitrides were characterized by powder X-ray diffraction and their crystal structures were refined by the Rietveld method. ZnSiN2 (a = 5.24637(4), b = 6.28025(5), c = 5.02228(4) Å, Z = 4, Rwp = 0.0556) and isotypic ZnGeN2 (a = 5...
April 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28426036/formation-of-the-intermetalloid-cluster-agsn18-7-the-reactivity-of-coinage-metal-nhc-compounds-towards-sn9-4
#8
F S Geitner, W Klein, T F Fässler
In recent years the formation of intermetalloid clusters by reacting homoatomic tetrel cluster anions with transition metal complexes has become a promising synthetic route. Nevertheless a better understanding of the processes occurring in solution is necessary. Here we present a series of novel polyanionic coinage metal NHC Zintl clusters [NHC(Dipp)M(η(4)-Sn9)](3-) (M: Cu, Ag, Au; Dipp: diisopropylphenyl) which are obtained at low temperatures from the reaction of K4Sn9 with NHC(Dipp)MCl (M: Cu-Au) in liquid ammonia...
April 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28425821/l-carnitine-supplementation-to-reverse-hyperammonemia-in-a-patient-undergoing-chronic-valproic-acid-treatment-a-case-report
#9
Cecilia Maldonado, Natalia Guevara, Alicia Silveira, Pietro Fagiolino, Marta Vázquez
Valproic acid is a broad-spectrum anticonvulsant that has also gained attention in the psychiatric setting. With respect to safety, valproic acid may induce a seemingly rare condition, hyperammonemia, which can induce a wide variety of symptoms ranging from irritability to coma. The proposed mechanism of hyperammonemia involves depletion of carnitine and overproduction of a toxic metabolite, 4-en-valproic acid, both of which impair the urea cycle and thus ammonia elimination. Carnitine is a commonly used antidote for acute intoxication of valproic acid, but is not a therapeutic option for management of chronic adults with adverse effects related to valproic acid...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28423995/harvesting-nutrients-from-source-separated-urine-using-powdered-rice-straw
#10
Shervin Hashemi, Mooyoung Han
As a principle of resource-oriented sanitation practice, urine should be separated from the source and utilized for other purposes such as producing fertilizer. This is because urine is rich in nutrients; therefore, sending it directly to wastewater treatment plants causes problems in the regular treatment process. The addition of solid additives such as powdered rice straw can help with harvesting nutrients from urine. In this study, the procedure and efficiency of using powdered rice straw for nutrient harvesting were investigated by tracking the reductions in ammonia, phosphate, magnesium, and calcium ions, and the harvested nutrients were identified using crystallography methods...
April 20, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28423881/effects-of-calcium-propionate-on-the-fermentation-quality-and-aerobic-stability-of-alfalfa-silage
#11
Zhi Hao Dong, Xian Jun Yuan, Ai You Wen, Seare T Desta, Tao Shao
Objective: To assess the potency of calcium propionate used as silage additive, an experiment was carried out to evaluate the effect of calcium propionate on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages. Methods: Alfalfa was ensiled with four levels of calcium propionate (CAP; 5, 10, 15 and 20 g kg-1 of fresh weigh) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28422839/unexpected-side-effect-in-mcrc-a-care-compliant-case-report-of-regorafenib-induced-hyperammonemic-encephalopathy
#12
Michela Quirino, Sabrina Rossi, Giovanni Schinzari, Michele Basso, Antonia Strippoli, Alessandra Cassano, Carlo Barone
RATIONALE: Regorafenib represents a treatment option in heavily pretreated patients affected by metastatic colorectal cancer (mCRC). Its safety profile is typical of small-molecule tyrosine-kinase inhibitors (TKIs) and most adverse events are manageable. PATIENT CONCERNS: A 56 years-old Caucasian man affected by mCRC with normal hepatic reserve was treated with regorafenib as second-line treatment. After only 2 days of therapy, the patient presented to the emergency department due to impairment of both spatial and temporal orientation and motor function with bradylalia...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28422044/design-of-flexible-pani-coated-cuo-tio2-sio2-heterostructure-nanofibers-with-high-ammonia-sensing-response-values
#13
Zengyuan Pang, Qingxin Nie, Pengfei Lv, Jian Yu, Fenglin Huang, Qufu Wei
We report a room temperature ammonia sensor with extra high response values and ideal flexibility, including polyaniline (PANI) coated titanium dioxide- silicon dioxide (TiO2-SiO2) or copper oxide- titanium dioxide- silicon dioxide (CuO-TiO2-SiO2) composite nanofibers. Such flexible inorganic TiO2-SiO2 and CuO-TiO2-SiO2 composite nanofibers were prepared by electrospinning, followed by calcination. Then in situ polymerization of aniline monomers was operated with inorganic TiO2-SiO2 and CuO-TiO2-SiO2 composite nanofibers as templates...
April 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28420743/cancer-cell-metabolism-the-essential-role-of-the-nonessential-amino-acid-glutamine
#14
REVIEW
Ji Zhang, Natalya N Pavlova, Craig B Thompson
Biochemistry textbooks and cell culture experiments seem to be telling us two different things about the significance of external glutamine supply for mammalian cell growth and proliferation. Despite the fact that glutamine is a nonessential amino acid that can be synthesized by cells from glucose-derived carbons and amino acid-derived ammonia, most mammalian cells in tissue culture cannot proliferate or even survive in an environment that does not contain millimolar levels of glutamine. Not only are the levels of glutamine in standard tissue culture media at least ten-fold higher than other amino acids, but glutamine is also the most abundant amino acid in the human bloodstream, where it is assiduously maintained at approximately 0...
April 18, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28419726/chemotaxonomic-characterization-of-the-thaumarchaeal-lipidome
#15
Felix J Elling, Martin Könneke, Graeme W Nicol, Michaela Stieglmeier, Barbara Bayer, Eva Spieck, José R de la Torre, Kevin W Becker, Michael Thomm, James I Prosser, Gerhard J Herndl, Christa Schleper, Kai-Uwe Hinrichs
Thaumarchaeota are globally distributed and abundant microorganisms occurring in diverse habitats and thus represent a major source of archaeal lipids. The scope of lipids as taxonomic markers in microbial ecological studies is limited by the scarcity of comparative data on the membrane lipid composition of cultivated representatives, including the phylum Thaumarchaeota. Here, we comprehensively describe the core and intact polar lipid (IPL) inventory of ten ammonia-oxidizing thaumarchaeal cultures representing all four characterized phylogenetic clades...
April 17, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28419436/ammonia-oxidizer-populations-vary-with-nitrogen-cycling-across-a-tropical-montane-mean-annual-temperature-gradient
#16
S Pierre, I Hewson, J P Sparks, C M Litton, C Giardina, P M Groffman, T J Fahey
Functional gene approaches have been used to better understand the roles of microbes in driving forest soil nitrogen (N) cycling rates and bioavailability. Ammonia oxidation is a rate limiting step in nitrification, and is a key area for understanding environmental constraints on N availability in forests. We studied how increasing temperature affects the role of ammonia oxidizing archaea (AOA) and bacteria (AOB) in soil N cycling and availability by using a highly constrained natural mean annual temperature (MAT) elevation gradient in a tropical montane wet forest...
April 18, 2017: Ecology
https://www.readbyqxmd.com/read/28418097/genome-and-epigenome-of-a-novel-marine-thaumarchaeota-strain-suggest-viral-infection-phosphorothioation-dna-modification-and-multiple-restriction-systems
#17
Nathan A Ahlgren, Yangyang Chen, David M Needham, Alma E Parada, Rohan Sachdeva, Vicki Trinh, Ting Chen, Jed A Fuhrman
Marine Thaumarchaeota are abundant ammonia-oxidizers but have few representative laboratory-cultured strains. We report the cultivation of Candidatus Nitrosomarinus catalina SPOT01, a novel strain that is less warm-temperature tolerant than other cultivated Thaumarchaeota. Using metagenomic recruitment, strain SPOT01 comprises a major portion of Thaumarchaeota (4-54%) in temperate Pacific waters. Its complete 1.36 Mbp genome possesses several distinguishing features: putative phosphorothioation (PT) DNA modification genes; a region containing probable viral genes; and putative urea utilization genes...
April 18, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28416262/use-of-dilute-ammonia-gas-for-treatment-of-1-2-3-trichloropropane-and-explosives-contaminated-soils
#18
Charles G Coyle, Scott A Waisner, Victor F Medina, Chris S Griggs
Laboratory studies were performed to test a novel reactive gas process for in-situ treatment of soils containing halogenated propanes or explosives. A soil column study, using a 5% ammonia-in-air mixture, established that the treatment process can increase soil pH from 7.5 to 10.2. Batch reactor experiments were performed to demonstrate contaminant destruction in sealed jars exposed to ammonia. Comparison of results from batch reactors that were, and were not, exposed to ammonia demonstrated reductions in concentrations of 1,2,3-trichloropropane (TCP), 1,3-dichloropropane (1,3-DCP), 1,2-dicholoropropane (1,2-DCP) and dibromochloropropane (DBCP) that ranged from 34 to 94%...
April 14, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28415039/removal-of-fluoride-sds-ammonia-and-turbidity-from-semiconductor-wastewater-by-combined-electrocoagulation-electroflotation
#19
S Aoudj, A Khelifa, N Drouiche
Semiconductor industry effluents contain organic and inorganic pollutants, such as sodium dodecyl sulfate (SDS), fluoride and ammonia, at high levels which consists a major environmental issue. A combined EC-EF process is proposed as a post-treatment after precipitation for simultaneous clarification and removal of pollutants. In EC step, a hybrid Fe-Al was used as the soluble anode in order to avoid supplementary EC step. EC-Fe is more suitable for SDS removal; EC-Al is more suitable for fluoride removal, while EC with hybrid Al-Fe makes a good compromise...
April 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28414666/just-a-puff-of-smoke
#20
A Leonard
CASE: A 44 year old woman with a history of stroke s/p aneurysm clipping, seizure, and substance abuse was brought to the hospital after a family member received a call from her friend saying she was acting unusual and may have had a seizure while hanging out on the street. At her baseline, she needed modest assistance in daily activities after her CVA and conversed without issue. Her mother died of a stroke. On exam the patient was afebrile, normotensive with mild tachycardia to 110...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
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