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Ammonia

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https://www.readbyqxmd.com/read/29346648/esrb-negatively-regulates-expression-of-the-glutamine-sythetase-glna-in-the-fish-pathogen-edwardsiella-piscicida
#1
Yunpeng Guan, Kaiyu Yin, Mian Zhou, Minjun Yang, Yuanxing Zhang, Xiaohong Liu, Qiyao Wang
Edwardsiella piscicida is a gram-negative bacterial pathogen invading a wide range of fish species. Response regulator EsrB is essential for activation of type III and type VI secretion systems (T3/T6SS). In this study, proteomes of the wild type E. piscicida EIB202 and a ΔesrB mutant strains were compared to identify the regulon components of EsrB cultured in DMEM allowing T3/T6SS expression. As a result, 19 proteins showed different expression, which were identified to be associated with T3/T6SS, related to amino acid transport and metabolism, and energy production...
January 15, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346327/enhanced-isotopic-ratio-outlier-analysis-iroa-peak-detection-and-identification-with-ultra-high-resolution-gc-orbitrap-ms-potential-application-for-investigation-of-model-organism-metabolomes
#2
Yunping Qiu, Robyn D Moir, Ian M Willis, Suresh Seethapathy, Robert C Biniakewitz, Irwin J Kurland
Identifying non-annotated peaks may have a significant impact on the understanding of biological systems. In silico methodologies have focused on ESI LC/MS/MS for identifying non-annotated MS peaks. In this study, we employed in silico methodology to develop an Isotopic Ratio Outlier Analysis (IROA) workflow using enhanced mass spectrometric data acquired with the ultra-high resolution GC-Orbitrap/MS to determine the identity of non-annotated metabolites. The higher resolution of the GC-Orbitrap/MS, together with its wide dynamic range, resulted in more IROA peak pairs detected, and increased reliability of chemical formulae generation (CFG)...
January 18, 2018: Metabolites
https://www.readbyqxmd.com/read/29345396/performance-rumen-fermentation-and-blood-metabolites-of-dairy-calves-fed-starter-mixtures-supplemented-with-herbal-plants-essential-oils-or-monensin
#3
M Akbarian-Tefaghi, E Ghasemi, M Khorvash
This study evaluated the supplementation effects of three herbal plants (thyme [THY], eucalyptus [EUC] and celery [CEL]), a commercial phytogenic additive ‎containing essential oils (PFA-EO, Digestarom® P.E.P.) and monensin (MON) in calf starter on performance, rumen fermentation and blood metabolites during pre- (days 3-55) and post-weaning (days 56-70). Sixty-six Holstein dairy calves (3 days of age, 41.2 ± 3 kg of BW) were allocated to one of six starters supplemented with: (i) no additives (CON), (ii) MON (30 mg/kg), (iii) THY (23 g/kg), (iv) CEL (23 g/kg), (v) EUC (23 g/kg) and (vi) PFA-EO (3 g/kg)...
January 18, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29343777/the-hidden-cost-of-using-low-resolution-concentration-data-in-the-estimation-of-nh3-dry-deposition-fluxes
#4
Frederik Schrader, Martijn Schaap, Undine Zöll, Richard Kranenburg, Christian Brümmer
Long-term monitoring stations for atmospheric pollutants are often equipped with low-resolution concentration samplers. In this study, we analyse the errors associated with using monthly average ammonia concentrations as input variables for bidirectional biosphere-atmosphere exchange models, which are commonly used to estimate dry deposition fluxes. Previous studies often failed to account for a potential correlation between ammonia exchange velocities and ambient concentrations. We formally derive the exact magnitude of these errors from statistical considerations and propose a correction scheme based on parallel measurements using high-frequency analysers...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343038/-diagnostic-value-of-albumin-bilirubin-grade-combined-with-serum-ammonia-in-cirrhosis-with-hepatic-encephalopathy
#5
Y L Hou, M D Gao, H Y Guo, R Wang, Z Wang, Y H Yu, J L Lou, Y Zhao
Objective: To explore the value of albumin-bilirubin (ALBI) grade combined with serum ammonia in the diagnosis of cirrhosis with hepatic encephalopathy (HE). Methods: The serum level of total bilirubin(TBIL), albumin( ALB )and blood ammonia were detected in 139 patients including 73 cirrhosis patients without HE and 66 cirrhosis patients with HE from January 2015 to January 2017 in Beijing You'an Hospital, and the relationship between ALBI and blood ammonia value and Child grade and hepatic encephalopathy was analyzed...
January 9, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29342234/stability-indicating-hplc-and-hptlc-methods-for-determination-of-agomelatine-and-its-degradation-products
#6
Maha M Abdelrahman, Ibrahim A Naguib, Mohamed R El Ghobashy, Nesma A Ali
Two accurate, sensitive and highly selective stability-indicating methods are developed and validated for simultaneous determination of Agomelatine (AGM) and its forced degradation products (Deg I and II). The first method is High-Performance Liquid Chromatography for separation and quantitation of AGM, Deg I and II on a C18 column (250 mm × 4.6 mm, 5 μm p.s) in isocratic mode by using a binary mixture of Potassium dihydrogen phosphate (0.05 M, pH adjusted to 2.9 with orthophosphoric acid): acetonitrile (60:40, v/v) at a flow rate of 2 mL/min...
January 12, 2018: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29341260/essential-oils-improved-weight-gain-growth-and-feed-efficiency-of-young-dairy-calves-fed-18-or-20-crude-protein-starter-diets
#7
M Kazemi-Bonchenari, R Falahati, M Poorhamdollah, S R Heidari, A Pezeshki
The objective was to evaluate interactions between starter protein (180 vs. 200 g/kg, DM basis) and a mixture of essential oils (EOs; containing thymol, eugenol, vanillin, limonene and guaiacol) on growth, metabolic and ruminal functions of Holstein dairy calves. In a completely randomized 2 × 2 factorial design, 48 calves, 3 days old (averaging BW 42.7 ± 1.9 kg), were allocated into groups fed the following diets: (i) 180 g/kg CP with no EO (180P-NEO); (ii) 180 g/kg CP with EO (180P-EO); (iii) 200 g/kg CP with no EO (200P-NEO); and (iv) 200 g/kg CP with EO (200P-EO)...
January 17, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29340956/semi-targeted-analysis-of-complex-matrices-by-esi-ft-icr-ms-or-how-an-experimental-bias-may-be-used-as-an-analytical-tool
#8
Jasmine Hertzog, Vincent Carré, Anthony Dufour, Frédéric Aubriet
Ammonia is well suited to favor deprotonation process in electrospray ionization mass spectrometry (ESI-MS) to increase the formation of [M - H]-. Nevertheless, NH3 may react with carbonyl compounds (aldehyde, ketone) and bias the composition description of the investigated sample. This is of significant importance in the study of complex mixture such as oil or bio-oil. To assess the ability of primary amines to form imines with carbonyl compounds during the ESI-MS process, two aldehydes (vanillin and cinnamaldehyde) and two ketones (butyrophenone and trihydroxyacetophenone) have been infused in an ESI source with ammonia and two different amines (aniline and 3-chloronaniline)...
January 16, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29339624/the-combined-effects-of-carbon-nitrogen-ratio-suspended-biomass-hydraulic-retention-time-and-dissolved-oxygen-on-nutrient-removal-in-a-laboratory-scale-anaerobic-anoxic-oxic-activated-sludge-biofilm-reactor
#9
D S Manu, Arun Kumar Thalla
The current trend in sustainable development deals mainly with environmental management. There is a need for economically affordable, advanced treatment methods for the proper treatment and management of domestic wastewater containing excess nutrients (such as nitrogen and phosphorus) which can cause eutrophication. The reduction of the excess nutrient content of wastewater by appropriate technology is of much concern to the environmentalist. In the current study, a novel integrated anaerobic-anoxic-oxic activated sludge biofilm (A2O-AS-biofilm) reactor was designed and operated to improve the biological nutrient removal by varying reactor operating conditions such as carbon to nitrogen (C/N) ratio, suspended biomass, hydraulic retention time (HRT) and dissolved oxygen (DO)...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29339619/improved-sludge-dewaterability-and-hydrolysis-performance-after-pretreatment-with-fenton-s-reagent
#10
Hongying Yuan, Yuping Yang, Jian Yuan, Yanning Wang, Yameng Song, Jingfang Lu, Jianyang Song
The dewaterability of excess sludge significantly improved upon pretreatment with Fenton's reagent in this study. After 0.9 g/L of Fe2+ and 5.0 g/L of H2O2 were added to the sludge, and reacted for 2 h at pH = 4, the specific resistance to filtration (SRF) of the excess sludge decreased from an initial value of 29.74 × 1012 m/kg to 6.49 × 1012 m/kg. The factors that affected this improvement in sludge dewaterability as evaluated by SRF reduction showed the following order: H2O2 > pH > Fe2+ > reaction time...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29339617/characteristics-of-n2o-production-and-hydroxylamine-variation-in-short-cut-nitrification-sbr-process
#11
Bo Hu, Junhong Ye, Jianqiang Zhao, Xiaoqian Ding, Liwei Yang, Xiaolei Tian
In order to study the characteristics of nitrous oxide (N2O) production and hydroxylamine (NH2OH) variation under oxic conditions, concentrations of NH2OH and N2O were simultaneously monitored in a short-cut nitrification sequencing batch reactor (SBR) operated with different influent ammonia concentrations. In the short-cut nitrification process, N2O production was increased with the increasing of ammonia concentration in influent. The maximum concentrations of dissolved N2O-N in the reactor were 0.11 mg/L and 0...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29339611/mechanism-of-1-4-dioxane-microbial-degradation-revealed-by-16s-rrna-and-metatranscriptomic-analyses
#12
Xiangyu Guan, Fei Liu, Jing Wang, Caoxiang Li, Xiaoxuan Zheng
1,4-Dioxane (dioxane), a probable human carcinogen, often exists in industrial wastewater and domestic sewage. In this study, we applied 16S rRNA and metatranscriptomic methods to analyze the dioxane biodegradation mechanism by activated sludge. Tetrahydrofuran (THF) was added as an essential co-metabolite to promote the degradation of dioxane. We found the dioxane removal ratio increased with increasing THF concentrations. When the THF concentration increased from 60.0 to 200.0 mg/L, the dioxane degradation rate was stable...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29338928/immunoproteomic-identification-of-antigenic-candidate-campylobacter-jejuni-and-human-peripheral-nerve-proteins-involved-in-guillain-barr%C3%A3-syndrome
#13
Aida Loshaj-Shala, Mara Colzani, Katerina Brezovska, Ana Poceva Panovska, Ljubica Suturkova, Giangiacomo Beretta
Immunoproteomics is become a potent methodology used for identifying immunoreactive proteins. In this study, an immunoproteomic approach based on 2-dimensional gel electrophoresis (2D-PAGE) and immunoblotting combined with high resolution mass spectrometry (MS) was used to identify immunoreactive proteins that might be involved in mechanisms of Guillain-Barré syndrome (GBS) development, regardless of their potential reciprocal molecular mimicry. Proteins isolated from C. jejuni and human peripheral nerve tissue (HPN) were separated with 2D SDS-PAGE and subjected to western blotting using serum samples from GBS patients...
January 9, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29338182/-infinite-sensitivity-of-black-silicon-ammonia-sensor-achieved-by-optical-and-electric-dual-drives
#14
Xiao-Long Liu, Suwan Zhu, Hai-Bin Sun, Yue Hu, Sheng-Xiang Ma, Xi-Jing Ning, Li Zhao, Jun Zhuang
The microstructured and hyperdoped silicon as a superior photoelectric and photovoltaic material is firstly studied as a gas-sensing material. The material is prepared by femtosecond-laser irradiation on Selenium-coated silicon and then fabricated as a conductive gas sensor targeting for ammonia. At room temperature, the sensitivity, response time, repeatability, distinguishability, selectivity, and natural aging effect of the sensor have been systematically studied. Results show that such black silicon has good potential for application as an ammonia-sensing material...
January 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29336182/the-effects-of-fermented-milk-containing-lactococcus-lactis-subsp-cremoris-fc-on-defaecation-in-healthy-young-japanese-women-a-double-blind-placebo-controlled-study
#15
Kaoru Ozaki, Toshinari Maruo, Hideki Kosaka, Mari Mori, Hideki Mori, Yukio Yamori, Toshiya Toda
The objective of this double-blind, placebo-controlled study was to elucidate the effects of fermented milk containing Lactococcus lactis subsp. cremoris FC (FC) on defaecation in healthy young women. We included 31 women (18-31 years old) who were randomly selected into two groups. Subjects in the test group consumed fermented milk containing FC, while subjects in the placebo group consumed non-fermented gelled milk. In the test group, defaecation frequency (both in days and times per week) and stool volume significantly increased during the consumption of fermented milk containing FC compared with before consumption...
January 16, 2018: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/29335552/a-pathway-for-biological-methane-production-using-bacterial-iron-only-nitrogenase
#16
Yanning Zheng, Derek F Harris, Zheng Yu, Yanfen Fu, Saroj Poudel, Rhesa N Ledbetter, Kathryn R Fixen, Zhi-Yong Yang, Eric S Boyd, Mary E Lidstrom, Lance C Seefeldt, Caroline S Harwood
Methane (CH4) is a potent greenhouse gas that is released from fossil fuels and is also produced by microbial activity, with at least one billion tonnes of CH4 being formed and consumed by microorganisms in a single year 1 . Complex methanogenesis pathways used by archaea are the main route for bioconversion of carbon dioxide (CO2) to CH4 in nature2-4. Here, we report that wild-type iron-iron (Fe-only) nitrogenase from the bacterium Rhodopseudomonas palustris reduces CO2 simultaneously with nitrogen gas (N2) and protons to yield CH4, ammonia (NH3) and hydrogen gas (H2) in a single enzymatic step...
January 15, 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29334654/phbv-polymer-supported-denitrification-system-efficiently-treated-high-nitrate-concentration-wastewater-denitrification-performance-microbial-community-structure-evolution-and-key-denitrifying-bacteria
#17
Zhongshuo Xu, Liyan Song, Xiaohu Dai, Xiaoli Chai
Biodegradable polymer supported denitrification (BPD) system shows good denitrification performance for the wastewater with low nitrate concentrations. In this study, a BPD system using Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) polymer as carbon source was developed to treat the wastewater with high nitrate concentrations. The denitrification performance, utilization ratio of PHBV polymers, and microbial community structure evolution and key denitrifying bacteria were comprehensively studied. Results indicated that an average nitrate removal efficiency of 99% could be achieved with an influent NO3--N concentration of 100 mg L-1 and a hydraulic retention time (HRT) of 7...
January 8, 2018: Chemosphere
https://www.readbyqxmd.com/read/29334633/fluorous-assisted-metal-chelate-affinity-extraction-for-nucleotides-followed-by-hilic-ms-ms-analysis
#18
Ena Kiyokawa, Tadashi Hayama, Hideyuki Yoshida, Masatoshi Yamaguchi, Hitoshi Nohta
We herein developed a selective method for the determination of nucleotides by fluorous-assisted metal chelate affinity extraction followed by hydrophilic interaction liquid chromatography (HILIC) combined with tandem mass spectrometric (MS/MS) analysis. In this study, the nucleotides were selectively chelated by Fe(III)-immobilized perfluoroalkyliminodiacetic acid, and the resulting chelates were subsequently extracted into a fluorous solvent. The nucleotides present in the fluorous solvent were then back-extracted into a non-fluorous solution, such as a solution of ammonia in aqueous acetonitrile...
January 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29334625/light-quality-affects-flavonoid-production-and-related-gene-expression-in-cyclocarya-paliurus
#19
Yang Liu, Shengzuo Fang, Wanxia Yang, Xulan Shang, Xiangxiang Fu
Understanding the responses of plant growth and secondary metabolites to differential light conditions is very important to optimize cultivation conditions of medicinal woody plants. As a highly valued and multiple function tree species, Cyclocarya paliurus is planted and managed for timber production and medical use. In this study, LED-based light including white light (WL), blue light (BL), red light (RL), and green light (GL) were used to affect leaf biomass production, flavonoid accumulation and related gene expression of one-year C...
January 11, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29334534/symptomatic-hyperammonemia-with-erwinia-chrysanthemi-derived-asparaginase-in-pediatric-leukemia-patients
#20
Nathan Gossai, Michael Richards, Lara Boman, Yoav Messinger, Sara Gernbacher, Joanna Perkins, Bruce Bostrom
Erwinia chrysanthemi-derived asparaginase is increasingly integral to acute lymphoblastic leukemia therapy. In our series, 16% of patients developed symptomatic hyperammonemia following Erwinia administration with symptoms including refractory nausea, vomiting, profound fatigue, malaise, and coma. This series of patients receiving Erwinia indicates higher than expected incidence of hyperammonemia, correlation between ammonia and asparaginase levels and therapeutic asparaginase activity levels despite dose reduction...
January 12, 2018: Journal of Pediatric Hematology/oncology
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