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Wenyao Chen, Xuezhi Duan, Gang Qian, De Chen, Xinggui Zhou
No abstract text is available yet for this article.
October 20, 2016: ChemSusChem
Yusif Abdullayev, Vagif Abbasov, Lucas C Ducati, Avtandil Talybov, Jochen Autschbach
The mechanisms of a tetrasubstituted imidazole [2-(2,4,5-triphenyl-1 H-imidazol-1-yl)ethan-1-ol] synthesis from benzil, benzaldehyde, ammonium acetate, and ethanolamine in [Et2NH2][HSO4] ionic liquid (IL) are studied computationally. The effects of the presence of the cationic and anionic components of the IL on transition states and intermediate structures, acting as a solvent versus as a catalyst, are determined. In IL-free medium, carbonyl hydroxylation when using a nucleophile (ammonia) proceeds with a Gibbs free energy (ΔG(≠)) barrier of 49...
October 2016: ChemistryOpen
Shahid Manzoor, Anna Schnürer, Erik Bongcam-Rudloff, Bettina Müller
Methanoculleus bourgensis strain MAB1 has been identified as the hydrogenotrophic partner of mesophilic acetate-oxidising bacteria, a syntrophic relationship operating close to the thermodynamic equilibrium and of considerable importance in ammonia-rich engineered biogas processes. Methanoculleus bourgensis strain MAB1 belongs to the order Methanomicrobiales, family Methanomicrobiaceae, within the phylum Euryarchaeota. The genome shows a total size of 2,859,299 bp encoding 3450 predicted protein-encoding genes, of which only 1472 (43 %) have been assigned tentative functions...
2016: Standards in Genomic Sciences
Christian Montes, Fabiola Altimira, Hayron Canchignia, Álvaro Castro, Evelyn Sánchez, María Miccono, Eduardo Tapia, Álvaro Sequeida, Jorge Valdés, Paz Tapia, Carolina González, Humberto Prieto
A new plant commensal Pseudomonas veronii isolate (strain R4) was identified from a Xiphinema index biocontrol screen. Isolated from grapevine roots from vineyards in central Chile, the strain R4 exhibited a slower yet equivalently effective nematicide activity as the well-characterized P. protegens CHA0. Whole genome sequencing of strain R4 and comparative analysis among the available Pseudomonas spp. genomes allowed for the identification of gene clusters that encode putative extracellular proteases and lipase synthesis and secretion systems, which are proposed to mediate-at least in part-the observed nematicidal activity...
2016: Standards in Genomic Sciences
Fei Cheng, Junyang Lu, Min Gao, Kai Shi, Qiusheng Kong, Yuan Huang, Zhilong Bie
Salicylic acid (SA) plays an important role in plant response to abiotic stresses. This study investigated the potential role of SA in alleviating the adverse effects of chilling stress on photosynthesis and growth in watermelon (Citrullus lanatus). Chilling stress induced the simultaneous accumulation of free and conjugated SA in watermelon plants, and the chilling-induced SA production was attributed to the phenylalanine ammonia-lyase pathway. Applying SA at moderate concentrations induced chilling tolerance, whereas inhibition of SA biosynthesis by L-α-aminooxy-β-phenylpropionic acid (AOPP) increased the photooxidation of PS II under chilling stress in watermelon, resulting in reduced photosynthesis and growth...
2016: Frontiers in Plant Science
Anna Carolina Formica-Oliveira, Ginés Benito Martínez-Hernández, Encarna Aguayo, Perla A Gómez, Francisco Artés, Francisco Artés-Hernández
Phenolic compounds are phytochemicals with high health-promoting properties. Carrot is a vegetable highly worldwide consumed although its phenolic content is low compared to other plant products. The aim of this work was to evaluate changes in phenolic compounds in carrots caused by abiotic stresses. The phenylalanine ammonia-lyase (PAL) activity, phenolic compounds and total antioxidant capacity (TAC) changes during storage up to 72 h at 15 °C after wounding (shredding), 9 kJ UV-C m(-2) pretreatment and hyperoxia (80 kPa) conditions of carrots were studied...
September 2016: Journal of Food Science and Technology
Mohammad Rahimi, Zachary Schoener, Xiuping Zhu, Fang Zhang, Christopher A Gorski, Bruce E Logan
A thermally regenerative ammonia battery (TRAB) recently developed for electricity generation using waste heat was adapted and used here as a treatment process for solutions containing high concentrations of copper ions. Copper removal reached a maximum of 77% at an initial copper concentration (Ci) of 0.05M, with a maximum power density (P) of 31Wm(-2)-electrode area. Lowering the initial copper concentration decreased the percentage of copper removal from 51% (Ci=0.01M, P=13Wm(-2)) to 2% (Ci=0.002M, P=2Wm(-2))...
October 13, 2016: Journal of Hazardous Materials
Xiaobing Zhou, Hilda Smith, Ana Giraldo Silva, Jayne Belnap, Ferran Garcia-Pichel
N2 fixation and ammonia oxidation (AO) are the two most important processes in the nitrogen (N) cycle of biological soil crusts (BSCs). We studied the short-term response of acetylene reduction assay (ARA) rates, an indicator of potential N2 fixation, and AO rates to temperature (T, -5°C to 35°C) in BSC of different successional stages along the BSC ecological succession and geographic origin (hot Chihuahuan and cooler Great Basin deserts). ARA in all BSCs increased with T until saturation occurred between 15 and 20°C, and declined at 30-35°C...
2016: PloS One
Mauro Zaninelli, Veronica Redaelli, Erica Tirloni, Cristian Bernardi, Vittorio Dell'Orto, Giovanni Savoini
The development of a monitoring system to identify the presence of laying hens, in a closed room of a free-range commercial organic egg production farm, was the aim of this study. This monitoring system was based on the infrared (IR) technology and had, as final target, a possible reduction of atmospheric ammonia levels and bacterial load. Tests were carried out for three weeks and involved 7 ISA (Institut de Sélection Animale) brown laying hens. The first 5 days was used to set up the detection sensor, while the other 15 days were used to evaluate the accuracy of the resulting monitoring system, in terms of sensitivity and specificity...
October 21, 2016: Sensors
Yi-Ju Hsu, Wen-Ching Huang, Chien-Chao Chiu, Yan-Lin Liu, Wan-Chun Chiu, Chun-Hui Chiu, Yen-Shuo Chiu, Chi-Chang Huang
Chili pepper is used as a food, seasoning and has been revered for its medicinal and health claims. It is very popular and is the most common spice worldwide. Capsaicin (CAP) is a major pungent and bioactive phytochemical in chili peppers. CAP has been shown to improve mitochondrial biogenesis and adenosine triphosphate (ATP) production. However, there is limited evidence around the effects of CAP on physical fatigue and exercise performance. The purpose of this study was to evaluate the potential beneficial effects of CAP on anti-fatigue and ergogenic functions following physiological challenge...
October 20, 2016: Nutrients
Kay Weng Choy, Nilika Wijeratne, James C G Doery
No abstract text is available yet for this article.
February 2016: Pathology
Alberto Vázquez-Salazar, George Tan, Amanda Stockton, Renato Fani, Arturo Becerra, Antonio Lazcano
The five-membered heterocyclic imidazole group, which is an essential component of purines, histidine and many cofactors, has been abiotically synthesized in different model experiments that attempt to simulate the prebiotic environment. The evolutionary significance of imidazoles is highlighted not only by its presence in nucleic acid components and in histidine, but also by experimental reports of its ability to restore the catalytic activity of ribozymes. However, as of today there are no reports of histidine in carbonaceous chondrites, and although the abiotic synthesis of His reported by Shen et al...
October 22, 2016: Origins of Life and Evolution of the Biosphere
Marli Dercksen, Marinus Duran, Lodewijk IJlst, Wim Kulik, Jos P N Ruiter, Arno van Cruchten, Mendel Tuchman, Ronald J A Wanders
BACKGROUND: N-acetylglutamate synthase (NAGS) plays a key role in the removal of ammonia via the urea cycle by catalyzing the synthesis of N-acetylglutamate (NAG), the obligatory cofactor in the carbamyl phosphate synthetase 1 reaction. Enzymatic analysis of NAGS in liver homogenates has remained insensitive and inaccurate, which prompted the development of a novel method. METHODS: UPLC-MS/MS was used in conjunction with stable isotope (N-acetylglutamic-2,3,3,4,4-d5 acid) dilution for the quantitative detection of NAG produced by the NAGS enzyme...
October 13, 2016: Molecular Genetics and Metabolism
Minjie Zhao, Michel Mozeika Arau Jo, Stéphanie Dal, Séverine Sigrist, Martine Bergaentzlé, David Ramanitrahasimbola, Charles Andrianjara, Eric Marchioni
This study describes a selective and effective pressurized liquid extraction (PLE) coupled with HPLC-DAD-ESI/MS method for the identification and quantification of three fructosazine analogues (FZAs), fructosazine, 2,6- and 2,5-deoxyfructosazine in Madeglucyl(®) (MG) which is an ammonia treated extract of Eugenia jambolana Lamarck seeds, and is the world's first anti-diabetic phytodrug. FZAs were extracted from MG by PLE using methanol as extraction solvent. The PLE extract was then analyzed directly by HPLC-DAD-ESI/MS without cleanup step...
October 13, 2016: Journal of Chromatography. A
Hulya Yalçin, Ines Valenta, Fatih Yalçin, Celia Corona-Villalobos, Nestor Vasquez, Joshua Ra, Nagehan Kucukler, Abdel Tahari, Iraklis Pozios, Yun Zhou, Martin Pomper, Theodore P Abraham, Thomas H Schindler, M Roselle Abraham
Vasodilator-induced transient left ventricular (LV) cavity dilation by positron emission tomography (PET) is common in patients with hypertrophic cardiomyopathy (HC). Because most patients with PET-LV cavity dilation lack obstructive epicardial coronary artery disease, we hypothesized that vasodilator-induced subendocardial hypoperfusion resulting from microvascular dysfunction underlies this result. To test this hypothesis, we quantified myocardial blood flow (MBF) (subepicardial, subendocardial, and global MBF) and left ventricular ejection fraction (LVEF) in 104 patients with HC without significant coronary artery disease, using (13)NH3-PET...
September 15, 2016: American Journal of Cardiology
Vincent Poot, Maaike Hoekstra, Mitchell A A Geleijnse, Mark C M van Loosdrecht, Julio Pérez
Partial nitritation was stably achieved in a bench-scale airlift reactor (1.5L) containing granular sludge. Continuous operation at 20 °C treating low-strength synthetic wastewater (50 mg N-NH4(+)/L and no COD) achieved nitrogen loading rates of 0.8 g N-NH4(+)/(L·d) during partial nitritation. The switch between nitrite-oxidizing bacteria (NOB) repression and NOB proliferation was observed when ammonium concentrations in the reactor were below 2-5 mg N-NH4(+)/L for DO concentrations lower than 4 mg O2/L at 20 °C...
October 11, 2016: Water Research
Zhaoyue Wang, Xuejing Bai, Xuena Guo, Xiuping He
2-Phenylethanol (2-PE) is widely used in food, perfume and pharmaceutical industry, but lower production in microbes and less known regulatory mechanisms of 2-PE make further study necessary. In this study, crucial genes like ARO8 and ARO10 of Ehrlich pathway for 2-PE synthesis and key transcription factor ARO80 in Saccharomyces cerevisiae were re-regulated using constitutive promoter; in the meantime, the effect of nitrogen source in synthetic complete (SC) medium with L-phenylalanine (L-Phe) on Aro8/Aro9 and Aro10 was investigated...
October 21, 2016: Journal of Industrial Microbiology & Biotechnology
Chandrashekhar Patil, Rahul Suryawanshi, Sunil Koli, Satish Patil
Aminocyclopropane-1-carboxylate deaminase (ACCD) producing microorganisms support plant growth under a variety of biotic and abiotic stress conditions such as drought, soil salinity, flooding, heavy metal pollution and phyto-pathogen attack. Available screening methods for ACCD give idea only about its primary microbial ACCD activity than the actual potential. In the present investigation, we have simply improved screening method by incorporating pH indicator dyes (phenol red and bromothymol blue) in ACC containing medium...
October 18, 2016: Journal of Microbiological Methods
Nail Altunay, Ramazan Gürkan, Ulaş Orhan
Acrylamide is a toxic species for human health, and is a Maillard reaction product which forms spontaneously in heat treatment process of foods. Therefore, a simple, fast and cost-effective method was developed for the indirect determination of acrylamide in processed foods particularly consumed by children. The method is based on ion-pairing of acrylamide with fluorescein (F(2-)) in presence of Ni(II) ions at pH 9.0, and then extraction of the formed ternary complex into micellar phase of poly(ethyleneglycol-mono-p-nonylphenylether) (PONPE 7...
December 1, 2016: Talanta
Chaoyun Hao, Zhiqiang Xia, Rui Fan, Lehe Tan, Lisong Hu, Baoduo Wu, Huasong Wu
BACKGROUND: Piper nigrum L., or "black pepper", is an economically important spice crop in tropical regions. Black pepper production is markedly affected by foot rot disease caused by Phytophthora capsici, and genetic improvement of black pepper is essential for combating foot rot diseases. However, little is known about the mechanism of anti- P. capsici in black pepper. The molecular mechanisms underlying foot rot susceptibility were studied by comparing transcriptome analysis between resistant (Piper flaviflorum) and susceptible (Piper nigrum cv...
October 21, 2016: BMC Genomics
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