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https://www.readbyqxmd.com/read/28806067/immunosensor-employing-stable-solid-1-amino-2-naphthyl-phosphate-and-ammonia-borane-toward-ultrasensitive-and-simple-point-of-care-testing
#1
Jeongwook Seo, Hyeri Ha, Seonhwa Park, Al-Monsur Jiaul Haque, Sinyoung Kim, Jung Min Joo, Haesik Yang
Biosensors for ultrasensitive point-of-care testing require dried reagents with long-term stability and a high signal-to-background ratio. Although ortho-substituted diaromatic dihydroxy and aminohydroxy compounds undergo fast redox reactions, they are not used as electrochemical signaling species because they are readily oxidized and polymerized by dissolved oxygen. In this report, stable, solid 1-amino-2-naphthyl phosphate (1A2N-P) and ammonia-borane (H3N-BH3) are respectively employed as a substrate for alkaline phosphatase (ALP) and a reductant for electrochemical-chemical (EC) redox cycling...
August 14, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28805913/feeding-distillers-grains-soybean-hulls-or-a-mixture-of-both-to-cows-as-a-forage-replacement-effects-on-intake-digestibility-and-ruminal-fermentation-characteristics
#2
W B Smith, K P Coffey, R T Rhein, E B Kegley, D Philipp, J G Powell, J D Caldwell, A N Young
Coproduct feedstuffs offer a unique and potentially profitable avenue for cattle feeding strategies. However, research is lacking in the evaluation of varying coproducts on ruminal fermentation and digestive characteristics when included as the major component of the diet of cows. Our objective was to determine the effect of coproduct feedstuffs as a forage replacement on digestive and fermentative characteristics of cows. Eight ruminally fistulated cows (672 ± 32.0 kg initial BW and approximately 9 yr of age) were stratified by BW and randomly allocated to 1 of 4 diets (2 cows∙diet∙period) in a 2-period study: soybean hulls (SH), distillers' dried grains with solubles (DG), an isoenergetic mixture of soybean hulls and distillers' dried grains with solubles (MX), or ad libitum hay plus 0...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805887/characterizing-ammonia-emissions-from-horses-fed-different-crude-protein-concentrations
#3
J Weir, H Li, L K Warren, E Macon, C Wickens
Evaluating impact of animal agriculture on air quality has been the focus of recent research. Ammonia (NH) volatilization occurs when undigested protein in feces and urea in urine is broken down by bacteria and enzymes. Information regarding NH emission from equine facilities is limited, and effects of CP intake on NH emissions have not been investigated. Nine mature geldings were used in a 3 × 3 replicated Latin square design study to determine effects of dietary CP on potential NH losses from feces and urine...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805655/transfer-of-cvd-grown-graphene-for-room-temperature-gas-sensors
#4
Federica Rigoni, Rishi Maiti, Camilla Baratto, Maurizio Donarelli, Jennifer MacLeod, Bahrati Gupta, Mei Lyu, Andrea Ponzoni, Giorgio Sberveglieri, Nunzio Motta, Guido Faglia
An easy transfer procedure to obtain graphene-based gas sensing devices operating at room temperature is presented. Starting from chemical vapor deposition (CVD)-grown graphene on copper foil, we obtained single layer graphene which could be transferred onto arbitrary substrates. In particular, we placed single layer graphene on top of a SiO<sub>2</sub>/Si substrate with pre-patterned Pt electrodes to realize a chemiresistor gas sensor able to operate at room temperature. The responses to ammonia (10, 20, 30 ppm) and nitrogen dioxide (1, 2, 3 ppm) are shown at different values of relative humidity, in dark and under 254 nm UV light...
August 14, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28804836/assessment-of-factor-xiii
#5
László Muszbek, Éva Katona, Adrienne Kerényi
Blood coagulation factor XIII (FXIII) is essential for maintaining hemostasis. The absence of FXIII results in severe bleeding diathesis, which without prophylaxis frequently leads to fatal bleeding. As the usual hemostasis screening tests remain normal, the diagnosis of FXIII deficiency needs specific tests. Here, we describe FXIII activity determination by the ammonia release assay, which is the first-line test in the diagnostic algorithm for FXIII deficiency. The method for another activity test, the undeservedly rarely used fibrin cross-linking assay, is also presented...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28803100/optimization-of-aqueous-ammonia-soaking-of-manure-fibers-by-response-surface-methodology-for-unlocking-the-methane-potential-of-swine-manure
#6
Anna Lymperatou, Hariklia N Gavala, Ioannis V Skiadas
Swine manure mono-digestion often results to economically non-feasible processes, due to the high dilution and ammonia concentration together with the low degradation rates it presents. The effects of different parameters of Aqueous Ammonia Soaking (AAS) as a pretreatment for improving the digestion of manure fibers when coupled to an ammonia removal step were investigated in this study. Response Surface Methodology was followed and the influence and interactions of the following AAS parameters were studied: NH3 concentration, duration and solid-to-liquid ratio...
July 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28802993/microbial-community-structure-and-activity-in-trace-element-contaminated-soils-phytomanaged-by-gentle-remediation-options-gro
#7
M Touceda-González, Á Prieto-Fernández, G Renella, L Giagnoni, A Sessitsch, G Brader, J Kumpiene, I Dimitriou, J Eriksson, W Friesl-Hanl, R Galazka, J Janssen, M Mench, I Müller, S Neu, M Puschenreiter, G Siebielec, J Vangronsveld, P S Kidd
Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe...
August 10, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28802978/gas-phase-hydration-of-glyoxylic-acid-kinetics-and-atmospheric-implications
#8
Ling Liu, Xiuhui Zhang, Zesheng Li, Yunhong Zhang, Maofa Ge
Oxocarboxylic acids are one of the most important organic species found in secondary organic aerosols and can be detected in diverse environments. But the hydration of oxocarboxylic acids in the atmosphere has still not been fully understood. Neglecting the hydration of oxocarboxylic acids in atmospheric models may be one of the most important reasons for the significant discrepancies between field measurements and abundance predictions of atmospheric models for oxocarboxylic acids. In the present paper, glyoxylic acid, as the most abundant oxocarboxylic acids in the atmosphere, has been selected as an example to study whether the hydration process can occur in the atmosphere and what the kinetic process of hydration is...
August 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28801651/discovery-of-novel-highly-active-and-stable-aspartate-dehydrogenases
#9
Hao Li, Taicheng Zhu, Liangtian Miao, Dan Zhang, Yongxian Li, Qi Li, Yin Li
Aspartate family amino acids (AFAAs) have important commercial values due to their wide spectrum of applications. Most if not all AFAAs are produced under aerobic conditions which is energy-intensive. To establish a cost-effective anaerobic process for production of AFAAs, it holds great promise to develop a new pathway enabling the conversion of oxoloacetate into aspartate through direct amination which is catalyzed by aspartate dehydrogenase (AspDH). Compared with the well studied aspartate aminotransferase and aspartate ammonia-lyase, only a few AspDHs are characterized till date, and failure to reproduce the high activity of AspDH from Rastonia eutropha documented in the literature encouraged us to screen and characterize novel AspDHs from different origins...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28801579/tnf%C3%AE-induced-up-regulation-of-na-k-2cl-cotransporter-nkcc1-in-hepatic-ammonia-clearance-and-cerebral-ammonia-toxicity
#10
Vitaly I Pozdeev, Elisabeth Lang, Boris Görg, Hans J Bidmon, Prashant V Shinde, Gerald Kircheis, Diran Herebian, Klaus Pfeffer, Florian Lang, Dieter Häussinger, Karl S Lang, Philipp A Lang
The devastating consequences of hepatic failure include hepatic encephalopathy, a severe, life threatening impairment of neuronal function. Hepatic encephalopathy is caused by impaired hepatic clearance of NH4(+). Cellular NH4(+) uptake is accomplished mainly by the Na(+),K(+),2Cl(-) cotransporter. Here we show that hepatic clearance of NH4(+) is impaired in TNFα deficient as well as TNFR1&TNFR2 double knockout mice, which both develop hyperammonemia. Despite impaired hepatic clearance of NH4(+), TNFα deficient mice and TNFR1 deficient mice were protected against acute ammonia intoxication...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28801215/nitrogen-removal-from-digested-slurries-using-a-simplified-ammonia-stripping-technique
#11
Giorgio Provolo, Francesca Perazzolo, Gabriele Mattachini, Alberto Finzi, Ezio Naldi, Elisabetta Riva
This study assessed a novel technique for removing nitrogen from digested organic waste based on a slow release of ammonia that was promoted by continuous mixing of the digestate and delivering a continuous air stream across the surface of the liquid. Three 10-day experiments were conducted using two 50-L reactors. In the first two, nitrogen removal efficiencies were evaluated from identical digestates maintained at different temperatures (30°C and 40°C). At the start of the first experiment, the digestates were adjusted to pH 9 using sodium hydroxide, while in the second experiment pH was not adjusted...
August 8, 2017: Waste Management
https://www.readbyqxmd.com/read/28801172/the-inhibitory-effects-of-free-ammonia-on-ammonia-oxidizing-bacteria-and-nitrite-oxidizing-bacteria-under-anaerobic-condition
#12
Wenting Qian, Yongzhen Peng, Xiyao Li, Qiong Zhang, Bin Ma
The free ammonia (FA) inhibition on ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) under anaerobic condition was investigated in this study. The results indicated that NOB was more sensitive to the FA anaerobic treatment than AOB. The FA anaerobic inhibition on nitrifier gradually heightened with the increase of FA concentration. Accompanied with FA concentration increase from 0 to 16.82mgNH3-N·L(-1) (the highest concentration adopted in this study), the activity of AOB reduced by 15...
August 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28800702/single-mo-atom-supported-on-defective-boron-nitride-monolayer-as-an-efficient-electrocatalyst-for-nitrogen-fixation-a-computational-study
#13
Jingxiang Zhao, Zhongfang Chen
The production of ammonia (NH3) from molecular dinitrogen (N2) under mild conditions is one of the most attractive and challenging process in chemistry. Here by means of density functional theory (DFT) computations, we systemically investigated the potential of single transition metal atoms (Sc to Zn, Mo, Ru, Rh, Pd, and Ag) supported on the experimentally available defective boron nitride (TM-BN) monolayer with a boron monovacancy, as a N2 fixation electrocatalyst. Our computations revealed that the single Mo atom supported by defective BN nanosheet exhibits the highest catalytic activity for N2 fixation at room temperature through an enzymatic mechanism with a quite low overpotential of 0...
August 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28800555/simultaneous-nitrogen-and-organics-removal-using-membrane-aeration-and-effluent-ultrafiltration-in-an-anaerobic-fluidized-membrane-bioreactor
#14
Yaoli Ye, Pascal E Saikaly, B E Logan
Dissolved methane and a lack of nutrient removal are two concerns for treatment of wastewater using anaerobic fluidized bed membrane bioreactors (AFMBRs). Membrane aerators were integrated into an AFMBR to form an aeration membrane fluidized bed membrane bioreactor (AeMFMBR) capable of simultaneous removal of organic matter and ammonia without production of dissolved methane. Good effluent quality was obtained with no detectable suspended solids, 93±5% of chemical oxygen demand (COD) removal to 14±11mg/L, and 74±8% of total ammonia (TA) removal to 12±3mg-N/L for domestic wastewater (COD of 193±23mg/L and TA of 49±5mg-N/L) treatment...
August 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28800534/molecular-understanding-of-the-interaction-of-methyl-hydrogen-sulfate-with-ammonia-dimethylamine-water
#15
Xia Sheng, Hailiang Zhao, Lin Du
Theoretical calculations at the B3LYP-D3/aug-cc-pVTZ (aug-cc-pV(T+d)Z for sulfur) level were used to investigate the contribution of methyl hydrogen sulfate (MHS) to new particle formation with the common atmospheric aerosol nucleation precursors including water (H2O), ammonia (NH3), and dimethylamine (DMA). A typical characteristic feature of the MHS-containing complexes is the formation of six- or eight-membered ring structures via SOH⋯O (MHS donor), OH⋯O/N (H2O donor) and NH⋯O/N (NH3/DMA donor). The stability of the complexes was evaluated based on the calculated binding energies...
August 4, 2017: Chemosphere
https://www.readbyqxmd.com/read/28800134/bacterial-volatile-ammonia-regulates-the-consumption-sequence-of-d-pinitol-and-d-glucose-in-a-fungus-associated-with-an-invasive-bark-beetle
#16
Fangyuan Zhou, Letian Xu, Shanshan Wang, Bo Wang, Qiaozhe Lou, Min Lu, Jianghua Sun
Interactions among microbial symbionts have multiple roles in the maintenance of insect-microbe symbiosis. However, signals mediating microbial interactions have been scarcely studied. In the classical model system of bark beetles and fungal associates, fungi increase the fitness of insects. However, not all interactions are mutualistic, some of these fungal symbionts compete for sugars with beetle larvae. How this antagonistic effect is alleviated is unknown, and recent research suggests potential roles of bacterial symbionts...
August 11, 2017: ISME Journal
https://www.readbyqxmd.com/read/28799305/comparison-of-rifaximin-plus-lactulose-with-the-lactulose-alone-for-the-treatment-of-hepatic-encephalopathy
#17
Kiran Ahire, Archana Sonawale
INTRODUCTION: Hepatic encephalopathy is challenging complication of liver dysfunction. Therapeutic treatment options for hepatic encephalopathy are currently limited and have appreciable risks and benefits associated with their use. Rifaximin is a novel anti microbiological agent with wide spectrum of activity that has shown promise as an alternative option for hepatic encephalopathy. AIMS AND OBJECTIVE: The present study was undertaken to compare the effectiveness of Rifaximin and Lactulose as a combination vs Lactulose alone, to compare the adverse effects and to study the rapidity of therapeutic effects of Rifaximin and Lactulose...
August 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28798884/spe-tlc-densitometric-quantification-of-selected-synthetic-food-dyes-in-liquid-foodstuffs-and-pharmaceutical-preparations
#18
Anna W Sobańska, Jarosław Pyzowski, Elżbieta Brzezińska
Selected synthetic food dyes (tartrazine, Ponceau 4R, Brilliant Blue, orange yellow, and azorubine) were isolated from liquid preparations (mouthwashes and beverages) by Solid Phase Extraction on aminopropyl-bonded silica with diluted aqueous sodium hydroxide as an eluent. The extraction step was followed by thin layer chromatography on silica gel 60 with chloroform-isopropanol-25% aq. ammonia 1 : 3 : 1 (v/v/v) as mobile phase and the densitometric quantification of dyes was achieved using quadratic calibration plots (R(2) > 0...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28797960/acute-toxic-effects-of-zinc-and-mercury-on-survival-standard-metabolism-and-metal-accumulation-in-juvenile-ridgetail-white-prawn-exopalaemon-carinicauda
#19
Chengsong Zhang, Kuijie Yu, Fuhua Li, Jianhai Xiang
Ridgetail white prawn (Exopalaemon carinicauda) is widely distributed in Chinese coastal zones, especially in the Yellow Sea and Bohai Sea. It is not only considered as an important economic species in China, but also taken as a potential indicator species for the environmental pollution in the estuaries. At present, the responses of this species to environmental toxicants, including trace metal are not well understood. In this study, the acute toxic effects of zinc (Zn) and mercury (Hg) on the survival, oxygen consumption, ammonia-N excretion, and metal accumulation were investigated in the juveniles of E...
August 7, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28797902/effects-of-microcystins-contamination-on-soil-enzyme-activities-and-microbial-community-in-two-typical-lakeside-soils
#20
Qing Cao, Alan D Steinman, Xiaomei Su, Liqiang Xie
A 30-day indoor incubation experiment was conducted to investigate the effects of different concentrations of microcystin (1, 10, 100 and 1000 μg eq. MC-LR L(-1)) on soil enzyme activity, soil respiration, physiological profiles, potential nitrification, and microbial abundance (total bacteria, total fungi, ammonia-oxidizing bacteria and archaea) in two lakeside soils in China (Soil A from the lakeside of Lake Poyanghu at Jiujiang; Soil B from the lakeside of Lake Taihu at Suzhou). Of the enzymes tested, only phenol oxidase activity was negatively affected by microcystin application...
August 7, 2017: Environmental Pollution
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