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https://www.readbyqxmd.com/read/28214450/in-silico-structural-and-functional-analysis-of-mesorhizobium-acc-deaminase
#1
Krishnendu Pramanik, Tithi Soren, Soumik Mitra, Tushar Kanti Maiti
Nodulation is one of the very important processes of legume plants as it is the initiating event of fixing nitrogen. Although ethylene has essential role in normal plant metabolism but it has also negative impact on plants particularly in nodule formation in legume plants. It is also produced due to a variety of biotic or abiotic stresses. 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase is a rhizobial enzyme which cleaves ACC (immediate precursor of ethylene) into α-ketobutyrate and ammonia. As a result, the level of ethylene from the plant cells is decreased and the negative impact of ethylene on nodule formation is reduced...
February 11, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28214440/achieving-short-cut-nitrification-and-denitrification-in-modified-intermittently-aerated-constructed-wetland
#2
Jie Hou, Ling Xia, Tao Ma, Yiqing Zhang, Yiyong Zhou, Xugang He
This study aim to enhance nitrogen removal performance via shifting nitrogen removal pathway from nitrate to nitrite pathway. It was demonstrated that nitrite pathway was successfully and stably achieved in CWs by using modified intermittent aeration control with aeration 20min/non-aeration 100min and reducing DO concentration during aeration, nitrite in the effluent could accumulate to over 70% of the total oxidized nitrogen. Q-PCR analysis showed that nitrifying microbial communities were optimized under the alternating anoxic and aerobic conditions, ammonia oxidizing bacteria increased from 7...
February 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214439/different-cultivation-methods-to-acclimatise-ammonia-tolerant-methanogenic-consortia
#3
Hailin Tian, Ioannis A Fotidis, Enrico Mancini, Irini Angelidaki
Bioaugmentation with ammonia tolerant-methanogenic consortia was proposed as a solution to overcome ammonia inhibition during anaerobic digestion process recently. However, appropriate technology to generate ammonia tolerant methanogenic consortia is still lacking. In this study, three basic reactors (i.e. batch, fed-batch and continuous stirred-tank reactors (CSTR)) operated at mesophilic (37°C) and thermophilic (55°C) conditions were assessed, based on methane production efficiency, incubation time, TAN/FAN (total ammonium nitrogen/free ammonia nitrogen) levels and maximum methanogenic activity...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28213542/plant-community-and-nitrogen-deposition-as-drivers-of-%C3%AE-and-%C3%AE-prokaryotic-diversity-in-reconstructed-oil-sands-soils-and-natural-boreal-forest-soils
#4
Jacynthe Masse, Cindy E Prescott, Sébastien Renaut, Yves Terrat, Sue J Grayston
The Athabasca Oil Sands deposit is one of the largest single oil deposits in the world. Following surface mining, companies are required to restore soil-like profiles that can support the previous land capabilities. The objective of this study was to assess if soil prokaryotic α- and β-diversity in oil-sands soils reconstructed 20-30 years previously, and planted to one of three vegetation types (coniferous or deciduous trees and grassland) were similar to those found in natural boreal-forest soils subject to wildfire disturbance...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28212513/simultaneous-removal-of-no-and-hg-0-over-ce-cu-modified-v2o5-tio2-based-commercial-scr-catalysts
#5
Guilong Chi, Boxiong Shen, Ranran Yu, Chuan He, Xiao Zhang
A series of novel Ce-Cu modified V2O5/TiO2 based commercial SCR catalysts were prepared via ultrasonic-assisted impregnation method for simultaneous removal of NO and elemental mercury (Hg(0)). Nitrogen adsorption, X-ray diffraction (XRD), temperature programmed reduction of H2 (H2-TPR) and X-ray photoelectron spectroscopy (XPS) were used to characterize the catalysts. 7% Ce-1% Cu/SCR catalyst exhibited the highest NO conversion efficiency (>97%) at 200-400°C, as well as the best Hg(0) oxidation activity (>75%) at 150-350°C among all the catalysts...
February 12, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28212351/black-box%C3%A2-gastrointestinal%C3%A2-tract-needs%C3%A2-and%C3%A2-prospects%C3%A2-of%C3%A2-gaining%C3%A2-insights%C3%A2-of%C3%A2-fate%C3%A2-of%C3%A2-fat-%C3%A2-protein-%C3%A2-and%C3%A2-starch%C3%A2-in%C3%A2-case%C3%A2-of%C3%A2-exocrine%C3%A2-pancreatic%C3%A2-insufficiency%C3%A2-by%C3%A2-using%C3%A2-fistulated%C3%A2-pigs
#6
REVIEW
Anne Mößeler, Josef Kamphues
Exocrine pancreatic insufficiency (EPI) results in the maldigestion and malabsorption of nutrients. The digestive processes in humans and other monogastric species like rat and pig are characterized by a predominantly enzymatic digestion within the small intestine and microbial fermentation located in the hindgut. For protein, it is doctrine that only prececally absorbed amino acids can be transferred to the amino acid pool of the host, while postileal absorption of nitrogen-containing compounds occurs mainly in the form of ammonia, being a burden rather than a benefit for the organism...
February 16, 2017: Nutrients
https://www.readbyqxmd.com/read/28211601/biogeography-of-nodulated-legumes-and-their-nitrogen-fixing-symbionts
#7
REVIEW
Janet I Sprent, Julie Ardley, Euan K James
Contents I. II. III. IV. V. VI. VII. VIII. References SUMMARY: In the last decade, analyses of both molecular and morphological characters, including nodulation, have led to major changes in our understanding of legume taxonomy. In parallel there has been an explosion in the number of genera and species of rhizobia known to nodulate legumes. No attempt has been made to link these two sets of data or to consider them in a biogeographical context. This review aims to do this by relating the data to the evolution of the two partners: it highlights both longitudinal and latitudinal trends and considers these in relation to the location of major land masses over geological time...
February 17, 2017: New Phytologist
https://www.readbyqxmd.com/read/28211331/sulfamethoxazole-and-cod-increase-abundance-of-sulfonamide-resistance-genes-and-change-bacterial-community-structures-within-sequencing-batch-reactors
#8
Xueping Guo, Weihai Pang, Chunling Dou, Daqiang Yin
The abundant microbial community in biological treatment processes in wastewater treatment plants (WWTPs) may potentially enhance the horizontal gene transfer of antibiotic resistance genes with the presence of antibiotics. A lab-scale sequencing batch reactor was designed to investigate response of sulfonamide resistance genes (sulI, sulII) and bacterial communities to various concentrations of sulfamethoxazole (SMX) and chemical oxygen demand (COD) of wastewater. The SMX concentrations (0.001 mg/L, 0.1 mg/L and 10 mg/L) decreased with treatment time and higher SMX level was more difficult to remove...
February 1, 2017: Chemosphere
https://www.readbyqxmd.com/read/28211189/influence-of-oxygen-availability-on-the-activities-of-ammonia-oxidizing-archaea
#9
Wei Qin, Kelley A Meinhardt, James W Moffett, Allan H Devol, E Virginia Armbrust, Anitra E Ingalls, David A Stahl
Recent studies point to the importance of oxygen (O2 ) in controlling the distribution and activity of marine ammonia-oxidizing archaea (AOA), one of the most abundant prokaryotes in the ocean. The AOA are associated with regions of low O2 tension in oceanic oxygen minimum zones (OMZs), and O2 availability is suggested to influence their production of the ozone-depleting greenhouse gas nitrous oxide (N2 O). We show that marine AOA available in pure culture sustain high ammonia oxidation activity at low μM O2 concentrations, characteristic of suboxic regions of OMZs (<10 µM O2 ), and that atmospheric concentrations of O2 may inhibit the growth of some environmental populations...
February 17, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28210889/correlation-between-physicochemical-properties-of-modified-clinoptilolite-and-its-performance-in-the-removal-of-ammonia-nitrogen
#10
Yingbo Dong, Hai Lin, Yinhai He
The physicochemical properties of the 24 modified clinoptilolite samples and their ammonia-nitrogen removal rates were measured to investigate the correlation between them. The modified clinoptilolites obtained by acid modification, alkali modification, salt modification, and thermal modification were used to adsorb ammonia-nitrogen. The surface area, average pore width, macropore volume, mecropore volume, micropore volume, cation exchange capacity (CEC), zeta potential, silicon-aluminum ratios, and ammonia-nitrogen removal rate of the 24 modified clinoptilolite samples were measured...
March 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28210833/energy-transfer-in-anabaena-variabilis-filaments-adapted-to-nitrogen-depleted-and-nitrogen-enriched-conditions-studied-by-time-resolved-fluorescence
#11
Aya Onishi, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto
Nitrogen is among the most important nutritious elements for photosynthetic organisms such as plants, algae, and cyanobacteria. Therefore, nitrogen depletion severely compromises the growth, development, and photosynthesis of these organisms. To preserve their integrity under nitrogen-depleted conditions, filamentous nitrogen-fixing cyanobacteria reduce atmospheric nitrogen to ammonia, and self-adapt by regulating their light-harvesting and excitation energy-transfer processes. To investigate the changes in the primary processes of photosynthesis, we measured the steady-state absorption and fluorescence spectra and time-resolved fluorescence spectra (TRFS) of whole filaments of the nitrogen-fixing cyanobacterium Anabaena variabilis at 77 K...
February 16, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28209893/localized-aliphatic-organic-material-on-the-surface-of-ceres
#12
M C De Sanctis, E Ammannito, H Y McSween, A Raponi, S Marchi, F Capaccioni, M T Capria, F G Carrozzo, M Ciarniello, S Fonte, M Formisano, A Frigeri, M Giardino, A Longobardo, G Magni, L A McFadden, E Palomba, C M Pieters, F Tosi, F Zambon, C A Raymond, C T Russell
Organic compounds occur in some chondritic meteorites, and their signatures on solar system bodies have been sought for decades. Spectral signatures of organics have not been unambiguously identified on the surfaces of asteroids, whereas they have been detected on cometary nuclei. Data returned by the Visible and InfraRed Mapping Spectrometer on board the Dawn spacecraft show a clear detection of an organic absorption feature at 3.4 micrometers on dwarf planet Ceres. This signature is characteristic of aliphatic organic matter and is mainly localized on a broad region of ~1000 square kilometers close to the ~50-kilometer Ernutet crater...
February 17, 2017: Science
https://www.readbyqxmd.com/read/28209350/n-decyl-s-trityl-r-cysteine-a-new-chiral-selector-for-green-ligand-exchange-chromatography-applications
#13
Andrea Carotti, Federica Ianni, Emidio Camaioni, Lucia Pucciarini, Maura Marinozzi, Roccaldo Sardella, Benedetto Natalini
In search for new enantioselectivity profiles, the N-decyl-S-trityl-(R)-cysteine [C10-(R)-STC] was synthesized through a one-step procedure and then hydrophobically adsorbed onto an octadecylsilica surface to generate a stable chiral stationary phase for ligand-exchange chromatography (CLEC-CSP) applications. The CLEC analysis was carried out on underivatized amino acids, by using a Cu(II) sulphate (1.0mM) containing aqueous eluent system. Most of the analysed compounds (34 out of 45) were enantiodiscriminated by the C10-(R)-STC-based CSP, with resolution factor (RS) values up to 8...
February 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28208762/investigation-of-pristine-graphite-oxide-as-room-temperature-chemiresistive-ammonia-gas-sensing-material
#14
Alexander G Bannov, Jan Prášek, Ondřej Jašek, Lenka Zajíčková
Graphite oxide has been investigated as a possible room-temperature chemiresistive sensor of ammonia in a gas phase. Graphite oxide was synthesized from high purity graphite using the modified Hummers method. The graphite oxide sample was investigated using scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, thermogravimetry and differential scanning calorimetry. Sensing properties were tested in a wide range of ammonia concentrations in air (10-1000 ppm) and under different relative humidity levels (3%-65%)...
February 9, 2017: Sensors
https://www.readbyqxmd.com/read/28208702/the-glutamate-dehydrogenase-pathway-and-its-roles-in-cell-and-tissue-biology-in-health-and-disease
#15
REVIEW
Andreas Plaitakis, Ester Kalef-Ezra, Dimitra Kotzamani, Ioannis Zaganas, Cleanthe Spanaki
Glutamate dehydrogenase (GDH) is a hexameric enzyme that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia while reducing NAD(P)⁺ to NAD(P)H. It is found in all living organisms serving both catabolic and anabolic reactions. In mammalian tissues, oxidative deamination of glutamate via GDH generates α-ketoglutarate, which is metabolized by the Krebs cycle, leading to the synthesis of ATP. In addition, the GDH pathway is linked to diverse cellular processes, including ammonia metabolism, acid-base equilibrium, redox homeostasis (via formation of fumarate), lipid biosynthesis (via oxidative generation of citrate), and lactate production...
February 8, 2017: Biology
https://www.readbyqxmd.com/read/28208293/electrochemical-enantioselective-recognition-in-a-highly-ordered-self-assembly-framework
#16
Yongxin Tao, Xiaogang Gu, Baozhu Yang, Linhong Deng, Liping Bao, Yong Kong, Fuqiang Chu, Yong Qin
Construction of convenient systems for isomer discrimination is of great importance for medical and life sciences. Here, we report a simple and effective chiral sensing device based on a highly ordered self-assembly framework. Cu(2+)-modified β-cyclodextrin (Cu-β-CD) was self-assembled to the ammonia-ethanol cotreated chitosan (ae-CS), and the highly ordered framework was gradually formed during the "re-growth" process of the shrinked ae-CS films. Tryptophan (Trp) isomers were well discriminated with the highly ordered framework by electrochemical approach...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28208249/flexible-passive-near-field-communication-tag-for-multigas-sensing
#17
P Escobedo, M M Erenas, N López-Ruiz, M A Carvajal, S Gonzalez-Chocano, I de Orbe-Payá, L F Capitán-Valley, A J Palma, A Martínez-Olmos
In this work we present a full-passive flexible multigas sensing tag for the determination of oxygen, carbon dioxide, ammonia, and relative humidity readable by a smartphone. This tag is based on near field communication (NFC) technology for energy harvesting and data transmission to a smartphone. The gas sensors show an optic response that is read through high-resolution digital color detectors. A white LED is used as the common optical excitation source for all the sensors. Only a reduced electronics with very low power consumption is required for the reading of the optical responses and data transmission to a remote user...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28205581/pathways-of-n-removal-and-n2o-emission-from-a-one-stage-autotrophic-n-removal-process-under-anaerobic-conditions
#18
Kai Li, Fang Fang, Han Wang, Chao Wang, Youpeng Chen, Jinsong Guo, Xixi Wang, Fuyang Jiang
To investigate the pathways of nitrogen (N) removal and N2O emission in a one-stage autotrophic N removal process during the non-aeration phase, biofilm from an intermittent aeration sequencing batch biofilm reactor (SBBR) and organic carbon-free synthetic wastewater were applied to two groups of lab-scale batch experiments in anaerobic conditions using a (15)N isotopic tracer and specific inhibitors, respectively. Then, the microbial composition of the biofilm was analysed using high-throughput sequencing...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28202306/nitrite-oxidation-kinetics-of-two-nitrospira-strains-the-quest-for-competition-and-ecological-niche-differentiation
#19
Norisuke Ushiki, Masaru Jinno, Hirotsugu Fujitani, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda
Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP...
February 12, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28202018/de-novo-biosynthesis-of-pterostilbene-in-an-escherichia-coli-strain-using-a-new-resveratrol-o-methyltransferase-from-arabidopsis
#20
Kyung Taek Heo, Sun-Young Kang, Young-Soo Hong
BACKGROUND: Pterostilbene, a structural analog of resveratrol, has higher oral bioavailability and bioactivity than that of the parent compound; but is far less abundant in natural sources. Thus, to efficiently obtain this bioactive resveratrol analog, it is necessary to develop new bioproduction systems. RESULTS: We identified a resveratrol O-methyltransferase (ROMT) function from a multifunctional caffeic acid O-methyltransferase (COMT) originating from Arabidopsis, which catalyzes the transfer of a methyl group to resveratrol resulting in pterostilbene production...
February 15, 2017: Microbial Cell Factories
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