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https://www.readbyqxmd.com/read/27913190/synthesis-and-label-free-characterization-of-a-bimolecular-pna-homo-quadruplex
#1
Brunella Pinto, Giulia Rusciano, Stefano D'Errico, Nicola Borbone, Antonio Sasso, Vincenzo Piccialli, Luciano Mayol, Giorgia Oliviero, Gennaro Piccialli
BACKGROUND: G-quadruplex DNA is involved in many physiological and pathological processes. Both clinical and experimental studies on DNA G-quadruplexes are slowed down by their enzymatic instability. In this frame, more stable chemically modified analogs are needed. METHODS: The bis-end-linked-(gggt)2 PNA molecule (BEL-PNA) was synthesized using in solution and solid phase synthetic approaches. Quadruplex formation was assessed by circular dichroism (CD) and surface enhanced Raman scattering (SERS)...
November 29, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27903339/-effect-of-goal-directed-haemodynamic-management-on-the-postoperative-outcome-in-elderly-patients-with-fragile-cardiac-function-undergoing-abdominal-surgery
#2
L S Zheng, E W Gu, X H Peng, L Zhang, Y Y Cao
Objective: To investigate the effect of goal-directed haemodynamic management based on stroke volume variation (SVV), cardiac index (CI) and mean arterial blood pressure (MAP) on the postoperative outcome in elderly patients with fragile cardiac function undergoing gastrointestinal surgery. Methods: Ninety patients with fragile cardiac function, aged 65-90 years old, ASAⅡ or Ⅲ, NYHA Ⅱor Ⅲ, scheduled for abdominal surgery were enrolled in this study.The patients were randomly assigned to two groups: Experience anesthesia group (group E, n=45) and goal-directed hemodynamic management group (G group, n=45)...
November 22, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27871631/-benefit-of-general-anesthesia-monitored-by-bispectral-index-compared-with-monitoring-guided-only-by-clinical-parameters-systematic-review-and-meta-analysis
#3
Carlos Rogério Degrandi Oliveira, Wanderley Marques Bernardo, Victor Moisés Nunes
BACKGROUND: The bispectral index parameter is used to guide the titration of general anesthesia; however, many studies have shown conflicting results regarding the benefits of bispectral index monitoring. The objective of this systematic review with meta-analysis is to evaluate the clinical impact of monitoring with the bispectral index parameter. METHODS: The search for evidence in scientific information sources was conducted during December 2013 to January 2015, the following primary databases: Medline/PubMed, LILACS, Cochrane, CINAHL, Ovid, SCOPUS and TESES...
November 18, 2016: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/27865353/maintenance-of-cytosolic-calcium-is-crucial-to-extend-l-arginine-therapeutic-benefits-during-continuous-dosing
#4
Srinidi Mohan, Lisa Harding
The therapeutic benefits associated with short-term l-arginine supplementation are lost during continuous dosing. AMP-activated protein kinase (AMPK) functional modulation has been correlated with l-arginine therapeutic effectiveness, and with tolerance development during continuous supplementation. However, the metabolic link that is responsible for AMPK functional modulation during continuous l-arginine exposure is currently not known. To explore this, we incubated HUVECs for 7 days with 100 μmol/L l-arginine, in the presence or absence of other agents; and monitored their effects for eNOS function, and on tolerance sparing effects (viz, cellular glucose accumulation, and oxidative stress)...
October 2016: Nutrition Research
https://www.readbyqxmd.com/read/27862734/a-dinuclear-mercury-ii-mediated-base-pair-in-dna
#5
Soham Mandal, Marian Hebenbrock, Jens Müller
The first dinuclear metal-mediated base pair containing divalent metal ions has been prepared. A combination of the neutral bis(monodentate) purine derivative 1,N(6) -ethenoadenine (ϵA), which preferentially binds two metal ions with a parallel alignment of the N-M bonds, and the canonical nucleobase thymine (T), which readily deprotonates in the presence of Hg(II) and thereby partially compensates the charge accumulation due to the two closely spaced divalent metal ions, yields the dinuclear T-Hg(II)2 -ϵA base pair...
November 15, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27860119/bioimpedance-monitoring-of-cellular-hydration-during-hemodialysis-therapy
#6
Leslie D Montgomery, Richard W Montgomery, Wayne A Gerth, Susie Q Lew, Michael D Klein, Julian M Stewart, Marvin S Medow, Manuel T Velasquez
Introduction The aim of this paper is to describe and demonstrate how a new bioimpedance analytical procedure can be used to monitor cellular hydration of End Stage Renal Disease (ESRD) patients during hemodialysis (HD). Methods A tetra-polar bioimpedance spectroscope (BIS), (UFI Inc., Morro Bay, CA), was used to measure the tissue resistance and reactance of the calf of 17 ESRD patients at 40 discrete frequencies once a minute during dialysis treatment. These measurements were then used to derive intracellular, interstitial, and intravascular compartment volume changes during dialysis...
November 8, 2016: Hemodialysis International
https://www.readbyqxmd.com/read/27843781/comparison-of-bispectral-index-monitoring-with-the-critical-care-pain-observation-tool-in-the-pain-assessment-of-intubated-adult-patients-after-cardiac-surgery
#7
Zahra Faritous, Arvin Barzanji, Rasoul Azarfarin, Behshid Ghadrdoost, Mohsen Ziyaeifard, Nahid Aghdaei, Mostafa Alavi
BACKGROUND: Detecting pain is crucial in sedated and mechanically ventilated patients, as they are unable to communicate verbally. OBJECTIVES: This study aimed to compare Bispectral index (BIS) monitoring with the Critical-care pain observation tool (CPOT) and vital signs for pain assessment during painful procedures in intubated adult patients after cardiac surgery. MATERIALS AND METHODS: Seventy consecutive patients who underwent cardiac surgery (coronary artery bypass graft or valvular surgery) were enrolled in the study...
August 2016: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/27837538/techniques-for-loading-technetium-99m-and-rhenium-186-188-radionuclides-into-preformed-liposomes-for-diagnostic-imaging-and-radionuclide-therapy
#8
Beth Goins, Ande Bao, William T Phillips
Liposomes can serve as carriers of radionuclides for diagnostic imaging and therapeutic applications. Herein, procedures are outlined for radiolabeling liposomes with the gamma-emitting radionuclide, technetium-99m ((99m)Tc), for noninvasive detection of disease and for monitoring the pharmacokinetics and biodistribution of liposomal drugs, and/or with therapeutic beta-emitting radionuclides, rhenium-186/188 ((186/188)Re), for radionuclide therapy. These efficient and practical liposome radiolabeling methods use a post-labeling mechanism to load (99m)Tc or (186/188)Re into preformed liposomes prepared in advance of the labeling procedure...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27836194/synthesis-structure-dna-protein-binding-and-cytotoxic-activity-of-a-rhodium-iii-complex-with-2-6-bis-2-benzimidazolyl-pyridine
#9
Roya Esteghamat-Panah, Hassan Hadadzadeh, Hossein Farrokhpour, Jim Simpson, Amir Abdolmaleki, Fatemeh Abyar
A new mononuclear rhodium(III) complex, [Rh(bzimpy)Cl3] (bzimpy = 2,6-bis(2-benzimidazolyl)pyridine), was synthesized and characterized by elemental analysis and spectroscopic methods. The molecular structure of the complex was confirmed by single-crystal X-ray crystallography. The interaction of the complex with fish sperm DNA (FS-DNA) was investigated by UV spectroscopy, emission titration, and viscosity measurement in order to evaluate the possible DNA-binding mode and to calculate the corresponding DNA-binding constant...
November 4, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27811407/improved-electrode-positions-for-local-impedance-measurements-in-the-lung-a-simulation-study
#10
Jakob Orschulik, Rudolf Petkau, Tobias Wartzek, Nadine Hochhausen, Michael Czaplik, Steffen Leonhardt, Daniel Teichmann
Impedance spectroscopy can be used to analyze the dielectric properties of various materials. In the biomedical domain, it is used as bioimpedance spectroscopy (BIS) to analyze the composition of body tissue. Being a non-invasive, real-time capable technique, it is a promising modality, especially in the field of lung monitoring. Unfortunately, up to now, BIS does not provide any regional lung information as the electrodes are usually placed in hand-to-hand or transthoracic configurations. Even though transthoracic electrode configurations are in general capable of monitoring the lung, no focusing to specific regions is achieved...
November 4, 2016: Physiological Measurement
https://www.readbyqxmd.com/read/27790356/moderate-hypothermia-and-its-effects-in-reducing-the-applied-dose-of-anesthetics-for-patients-with-opium-dependence-in-cardiac-surgery-a-randomized-controlled-trial
#11
Reza Jalaian Taghadomi, Ebrahim Golmakani, Kambiz Alizadeh, Behrooz Mottahedi, Ali Rahdari, Shima Sheybani
BACKGROUND: An increasing number of patients addicted to opium are experiencing awareness during coronary artery bypass surgery (CABG) as a result of tolerance to anesthetics. OBJECTIVES: This research was primarily intended to determine the potential diminishing effects of moderate hypothermia on anesthetic dosage and recall of anesthesia during the procedure. METHODS: In this double-blind randomized controlled trial, a total of 80 CABG candidates with known addiction to opium were divided into two groups: one normothermic (N) and the other moderately hypothermic (H), both undergoing induction as well as close monitoring from September 2014 to January 2016...
September 2016: Electronic Physician
https://www.readbyqxmd.com/read/27776261/biosynthesis-of-palladium-nanoparticles-by-using-moringa-oleifera-flower-extract-and-their-catalytic-and-biological-properties
#12
K Anand, C Tiloke, A Phulukdaree, B Ranjan, A Chuturgoon, S Singh, R M Gengan
The biosynthesis of nanostructured biopalladium nanoparticles (PdNPs) from an aqueous solution of crystalline palladium acetate is reported. For the synthesised PdNPs in solution, an agroforest biomass waste petal of Moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. Continuous absorption in the UV region and subsequent brown colour change confirmed the formation of PdNPs. A strong surface plasmon peak for PdNPs occurred at 460nm. PdNPs were characterized by SEM with EDX, FTIR, TEM and DLS...
September 29, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27769482/polypeptide-with-electroactive-endgroups-as-sensing-platform-for-the-abused-drug-methamphetamine-by-bioelectrochemical-method
#13
Bilal Demir, Tulay Yilmaz, Emine Guler, Z Pinar Gumus, Huseyin Akbulut, Ebru Aldemir, Hakan Coskunol, Demet Goen Colak, Ioan Cianga, Shuhei Yamada, Suna Timur, Takeshi Endo, Yusuf Yagci
Affinity-type sensors have emerged as outstanding platforms in the detection of diagnostic protein markers, nucleic acids and drugs. Thus, these novel platforms containing antibodies could be integrated into the monitoring systems for abused drugs. Herein, we established a novel detection platform for the analysis of a common illicit drug; methamphetamine (METH). Initially, a fluorescent-labeled polypeptide (EDOT-BTDA-Pala), derived from L-alanine N-carboxyanhydride (L-Ala-NCA) via ring-opening polymerization using 4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)benzo[c][1,2,5]thiadiazole-5,6-diamine (EDOT-NH2-BTDA) as initiator, was employed as a glassy carbon electrode (GCE) covering host, in order to immobilize the METH-selective antibody...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27757457/xps-enables-visualization-of-electrode-potential-screening-in-an-ionic-liquid-medium-with-temporal-and-lateral-resolution
#14
M T Camci, P Aydogan, B Ulgut, C Kocabas, S Suzer
We present an X-ray photoelectron spectroscopic (XPS) investigation of potential screening across two gold electrodes fabricated on a porous polymer surface which is impregnated with the ionic liquid (IL) N-N-diethyl-N-methyl-N-(2-methoxyethyl)ammonium bis(trifluoromethanesulfonyl)imide [DEME-TFSI]. The IL provides a sheet of conducting layers to the insulating polymer film, and allows monitoring charging and screening dynamics at the polymer + IL/vacuum interface in a laterally resolved fashion across the electrodes...
October 19, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27746557/effect-of-bispectral-index-versus-end-tidal-anesthetic-gas-concentration-guided-protocol-on-time-to-tracheal-extubation-for-halothane-based-general-anesthesia
#15
Neena Jain, Pooja Rawat Mathur, Shoyeb Khan, Arvind Khare, Veena Mathur, Surendra Sethi
BACKGROUND AND AIMS: Early extubation is a desirable goal after general anesthesia. Very few studies have compared the effect of bispectral index (BIS) monitoring versus standard end-tidal anesthetic gas (ETAG) concentration monitoring on tracheal extubation time for halothane-based anesthesia. The aim of this study was to compare the effect of BIS versus ETAG-guided anesthesia on time to tracheal extubation for halothane-based anesthesia in general surgical setting. METHODS: This was a randomized, controlled double-blind study...
September 2016: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/27739307/high-anti-fouling-property-of-ion-selective-membrane-towards-in-vivo-monitoring-of-ph-change-in-live-brain-of-rats-with-membrane-coated-carbon-fiber-electrodes
#16
Jie Hao, Tongfang Xiao, Fei Wu, Ping Yu, Lanqun Mao
In vivo monitoring of pH in live brain remains very essential to understanding acid-base chemistry in various physiological processes. This study demonstrates a potentiometric method for in vivo monitoring of pH in the central nervous system with carbon fiber-based proton-selective electrodes (CF-H+ISEs) with high anti-fouling property. The CF-H+ISEs are prepared by formation of a H+-selective membrane (H+ISM) with polyvinyl chloride polymeric matrixes containing plasticizer bis(2-ethylhexyl)sebacate, H+ ionophore tridodecylamine, and ion exchanger potassium tetrakis(4-chlorophenyl)borate onto carbon fiber electrodes (CFEs)...
October 14, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27726196/evaluation-of-mdrd4-ckd-epi-bis-1-and-modified-cockcroft-gault-equations-to-estimate-glomerular-filtration-rate-in-the-elderly-renal-transplanted-recipients
#17
Elias David-Neto, Ana Heloisa Kamada Triboni, Fernanda Ramos, Fabiana Agena, Nelson Zocoler Galante, Marcelo Altona, Francine Brambate Carvalhinho Lemos, Marcelo Tatit Sapienza, William Carlos Nahas
Equations to estimate glomerular filtration rate (eGFR) were developed in patients using the variables age, body weight, and serum creatinine, which may be different in the elderly. Elderly renal transplant patients (EG; n=70; mean age 65 ± 4 y) who measured plasma (51) Cr-EDTA-Clearance (mGFR) had mGFR compared to eGFR obtained by the Cockcroft-Gault corrected by body surface area (CG-BSA), the modification of diet in renal disease (MDRD-4), the Berlin Initiative Study (BIS-1), and the chronic kidney disease epidemiology collaboration (CKD-EPI)...
December 2016: Clinical Transplantation
https://www.readbyqxmd.com/read/27714275/photocatalytic-degradation-of-ibuprofen-using-tio2-sensitized-by-ru-ii-polyaza-complexes
#18
J F Góngora, P Elizondo, A Hernández-Ramírez
In this work, modification of TiO2 was carried out by incorporation of two novel Ru(ii) polyaza complexes. The N(1)-(2-aminobenzyliden)-N(2),N(2)-bis(2-(2-aminobenzyliden)aminoethyl)ethane-1,2-diaminoruthenium(ii) and N(1),N(2)-bis(2-aminobenziliden)ethane-1,2-diaminoruthenium(ii) complexes were synthesized via metal-ligand direct reaction. The complexes were characterized by UV-Vis, FTIR and fluorescence spectroscopy, and the chemical composition was obtained from elemental analysis by the combustion method; additionally, the sensitized TiO2 catalysts were also characterized by XRD, SEM and diffuse reflectance techniques...
September 21, 2016: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/27704643/fragmentation-pathways-and-structural-characterization-of-organophosphorus-compounds-related-to-cwc-by-electron-ionization-and-electrospray-ionization-tandem-mass-spectrometry
#19
Seyed Esmaeil Hosseini, Hamid Saeidian, Ali Amozadeh, Mohammad Taghi Naseri, Mehran Babri
RATIONALE: For unambiguous identification of Chemical Weapons Convention (CWC)-related chemicals in environmental samples, the availability of mass spectra, interpretation skills and rapid microsynthesis of suspected chemicals are essential requirements. For the first time, the electron-ionization single quadrupole and electrospray ionization tandem mass spectra of a series of O-alkyl N-[bis(dimethylamino)methylidene]-P-methylphosphonamidates (Scheme 1, cpd 4) were studied for CWC verification purposes...
October 5, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27701366/comparison-of-intravenous-dexmedetomidine-and-midazolam-for-bispectral-index-guided-sedation-during-spinal-anesthesia
#20
Youn Yi Jo, Dongchul Lee, Wol Seon Jung, Noo Ree Cho, Hyun Jeong Kwak
BACKGROUND Despite the high frequency of hypotension during spinal anesthesia with proper sedation, no previous report has compared the hemodynamic effects of dexmedetomidine and midazolam sedation during spinal anesthesia. We compared the effects of bispectral index (BIS)-guided intravenous sedation using midazolam or dexmedetomidine on hemodynamics and recovery profiles in patients who underwent spinal anesthesia. MATERIAL AND METHODS One hundred and sixteen adult patients were randomly assigned to receive either midazolam (midazolam group; n=58) or dexmedetomidine (dexmedetomidine group; n=58) during spinal anesthesia...
October 4, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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