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Extracellular matric

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https://www.readbyqxmd.com/read/28914792/impact-of-source-and-manufacturing-of-collagen-matrices-on-fibroblast-cell-growth-and-platelet-aggregation
#1
Stefanie Böhm, Christine Strauß, Stefan Stoiber, Cornelia Kasper, Verena Charwat
Collagen is a main component of the extracellular matrix. It is often used in medical applications to support tissue regeneration, hemostasis, or wound healing. Due to different sources of collagen, the properties and performance of available products can vary significantly. In this in vitro study, a comparison of seven different collagen matrices derived from bovine, equine, and porcine sources was performed. As performance indicators, the scaffold function for fibroblasts and platelet aggregation were used...
September 15, 2017: Materials
https://www.readbyqxmd.com/read/28912695/region-and-cell-specific-expression-of-transmembrane-collagens-in-mouse-brain
#2
Aboozar Monavarfeshani, Courtney N Knill, Ubadah Sabbagh, Jianmin Su, Michael A Fox
Unconventional collagens are nonfribrillar proteins that not only contribute to the structure of extracellular matrices but exhibit unique bio-activities. Although roles for unconventional collagens have been well-established in the development and function of non-neural tissues, only recently have studies identified roles for these proteins in brain development, and more specifically, in the formation and refinement of synaptic connections between neurons. Still, our understanding of the full cohort of unconventional collagens that are generated in the mammalian brain remains unclear...
2017: Frontiers in Integrative Neuroscience
https://www.readbyqxmd.com/read/28910078/bifacial-biomimetic-freestanding-anisotropic-catechol-interfaces-with-asymmetrically-layered-films-as-versatile-extracellular-matrix-substitutes
#3
Byung Gee Im, Minjae Do, Yoojin Kim, Mira Cho, Jae-Hyung Jang
Biological naïve extracellular matrices (ECMs) exhibit anisotropic functions in their physical, chemical, and morphological properties. Representative examples include anisotropic skin layers or blood vessels simultaneously facing multiphasic environments. Here, anisotropically multifunctional structures called BiFACIAL (biomimetic freestanding anisotropic catechol-interfaces with asymmetrically layered) films were developed simply by contacting two polysaccharide solutions of heparin-catechol (Hep-C) and chitosan-catechol (Chi-C)...
September 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28904991/bioelectrospray-methodology-for-dissection-of-the-host-pathogen-interaction-in-human-tuberculosis
#4
Liku B Tezera, Magdalena K Bielecka, Paul T Elkington
Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the importance of modelling disease in a physiologically relevant 3-D environment. One of the approaches for 3-D cell culture is bioelectrospray technology. This technique uses an alginate-based 3-D environment as an inert backbone within which mammalian cells and extracellular matrix can be incorporated...
July 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28890327/induction-of-interdigitating-cell-processes-in-podocyte-culture
#5
Eishin Yaoita, Yutaka Yoshida, Masaaki Nameta, Hiroki Takimoto, Hidehiko Fujinaka
Highly organized cell processes characterize glomerular podocytes in vivo. However, podocytes in culture have a simple morphology lacking cell processes, especially upon reaching confluence. Here, we aimed to establish culture conditions under which cultured podocytes extend cell processes at confluence. Among various culture conditions that could possibly cause phenotypic changes in podocytes, we examined the effects of heparin, all-trans retinoic acid, fetal bovine serum, and extracellular matrices on the morphology of podocytes in rat primary culture...
September 7, 2017: Kidney International
https://www.readbyqxmd.com/read/28887084/tethered-growth-factors-on-biocompatible-scaffolds-improve-stemness-of-cultured-rat-and-human-neural-stem-cells-and-growth-of-oligodendrocyte-progenitors
#6
Lisamarie Moore, Nolan B Skop, Deborah E Rothbard, Lucas R Corrubia, Steven W Levison
Currently, there is no widely accepted technique to efficiently and reproducibly grow stem and progenitor cells in vitro. Stem cells require contact with extracellular matrices as well as signals from growth factors to proliferate and to retain their stemness. We have developed a novel tissue culture platform (StemTrix cultureware) that transforms standard tissue culture plasticware into a multi-functional chitosan-based scaffold that supports the expansion of neural stem cells. The StemTrix scaffold is comprised of chitosan with immobilized heparin which in turn tethers heparin-binding growth factors...
September 5, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28883544/global-fibroblast-activation-throughout-the-left-ventricle-but-localized-fibrosis-after-myocardial-infarction
#7
Chandan K Nagaraju, Eef Dries, Natasa Popovic, Abhishek A Singh, Peter Haemers, H Llewelyn Roderick, Piet Claus, Karin R Sipido, Ronald B Driesen
Fibroblast (Fb) differentiation and interstitial fibrosis contribute to cardiac remodeling and loss of function after myocardial infarction (MI). We investigated regional presence and regulation of fibrosis in a pig MI model. In vivo analysis of regional function and perfusion defined three regions: the scar, the myocardium adjacent to the scar (MIadjacent, reduced function, reduced perfusion reserve), and the remote myocardium (MIremote, minimal functional deficit, maintained perfusion). Interstitial and perivascular fibrosis, and increase of collagen type I, was only observed in the MIadjacent...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28881428/densification-of-type-i-collagen-matrices-as-a-model-for-cardiac-fibrosis
#8
Logan J Worke, Jeanne E Barthold, Benjamin Seelbinder, Tyler Novak, Russell P Main, Sherry L Harbin, Corey P Neu
Cardiac fibrosis is a disease state characterized by excessive collagenous matrix accumulation within the myocardium that can lead to ventricular dilation and systolic failure. Current treatment options are severely lacking due in part to the poor understanding of the complexity of molecular pathways involved in cardiac fibrosis. To close this gap, in vitro model systems that recapitulate the defining features of the fibrotic cellular environment are in need. Type I collagen, a major cardiac extracellular matrix protein and the defining component of fibrotic depositions, is an attractive choice for a fibrosis model, but demonstrates poor mechanical strength due to solubility limits...
September 7, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28869335/generation-and-evaluation-of-novel-stromal-cell-containing-tissue-engineered-artificial-stromas-for-the-surgical-repair-of-abdominal-defects
#9
M A Martin-Piedra, I Garzón, A Gómez-Sotelo, E Garcia-Abril, B D Jaimes-Parra, M López-Cantarero, M Alaminos, A Campos
BACKGROUND: Repair of abdominal wall defects is one of the major clinical challenges in abdominal surgery. Most biomaterials are associated to infection and severe complications, making necessary safer and more biocompatible approaches. AIM: In the present work, we combined the adequate mechanical properties of synthetic polymer meshes with tissue-engineered matrices containing stromal mesenchymal cells to generate a novel cell-containing tissue-like artificial stroma (SCTLAS) for use in abdominal wall repair...
September 4, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28864249/hydrogel-substrate-stress-relaxation-regulates-the-spreading-and-proliferation-of-mouse-myoblasts
#10
Aline Bauer, Luo Gu, Brian Kwee, Aileen Li, Maxence Dellacherie, Adam D Celiz, David J Mooney
Mechanical properties of the extracellular microenvironment are known to alter cellular behavior, such as spreading, proliferation or differentiation. Previous studies have primarily focused on studying the effect of matrix stiffness on cells using hydrogel substrates that exhibit purely elastic behavior. However, these studies have neglected a key property exhibited by the extracellular matrix (ECM) and various tissues; viscoelasticity and subsequent stress-relaxation. As muscle exhibits viscoelasticity, stress-relaxation could regulate myoblast behavior such as spreading and proliferation, but this has not been previously studied...
August 29, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28863344/elimination-of-antibiotic-resistance-bacterium-and-its-associated-dissociative-blatem-1-and-aac-3-ii-antibiotic-resistance-genes-in-aqueous-system-via-photoelectrocatalytic-process
#11
Qi Jiang, Hongliang Yin, Guiying Li, Hongli Liu, Taicheng An, Po Keung Wong, Huijun Zhao
The ubiquity of antibiotic-resistance bacteria (ARB) and antibiotic-resistance genes (ARGs) in various environmental matrices is a potential threat to human and ecological health. Therefore, the inactivation of ARB E. coli S1-23 and the elimination of its associated ARGs, blaTEM-1 and aac(3)-II, were investigated using the photoelectrocatalytic (PEC) process. Results indicate that the ARB E. coli S1-23 (1 × 10(8) cfu mL(-1)) and its ARGs (extracellular and intracellular) could be fully inactivated within 10 and 16 h PEC treatment, respectively...
August 23, 2017: Water Research
https://www.readbyqxmd.com/read/28860484/biocomposite-nanofiber-matrices-to-support-ecm-remodeling-by-human-dermal-progenitors-and-enhanced-wound-closure
#12
Fraz Anjum, Natacha A Agabalyan, Holly D Sparks, Nicole L Rosin, Michael S Kallos, Jeff Biernaskie
Cell-based therapies have recently been the focus of much research to enhance skin wound healing. An important challenge will be to develop vehicles for cell delivery that promote survival and uniform distribution of cells across the wound bed. These systems should be stiff enough to facilitate handling, whilst soft enough to limit damage to newly synthesized wound tissue and minimize patient discomfort. Herein, we developed several novel modifiable nanofibre scaffolds comprised of Poly (ε-caprolactone) (PCL) and gelatin (GE)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860021/disruption-of-fibronectin-matrix-affects-type-iv-collagen-fibrillin-and-laminin-deposition-into-extracellular-matrix-of-human-trabecular-meshwork-htm-cells
#13
Mark S Filla, Kaylee D Dimeo, Tiegang Tong, Donna M Peters
Fibronectin fibrils are a major component of the extracellular matrix (ECM) of the trabecular meshwork (TM). They are a key mediator of the formation of the ECM which controls aqueous humor outflow and contributes to the pathogenesis of glaucoma. The purpose of this work was to determine if a fibronectin-binding peptide called FUD, derived from the Streptococcus pyogenes Functional Upstream Domain of the F1 adhesin protein, could be used to control fibronectin fibrillogenesis and hence ECM formation under conditions where its expression was induced by treatment with the glucocorticoid dexamethasone...
August 30, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28843153/inactivation-of-three-genera-of-dominant-fungal-spores-in-groundwater-using-chlorine-dioxide-effectiveness-influencing-factors-and-mechanisms
#14
Gang Wen, Xiangqian Xu, Tinglin Huang, Hong Zhu, Jun Ma
Fungi in aquatic environments received more attention recently; therefore, the characteristics of inactivation of fungal spores by widely used disinfectants are quite important. Nonetheless, the inactivation efficacy of fungal spores by chlorine dioxide is poorly known. In this study, the effectiveness of chlorine dioxide at inactivation of three dominant genera of fungal spores isolated from drinking groundwater and the effects of pH, temperature, chlorine dioxide concentration, and humic acid were evaluated...
August 18, 2017: Water Research
https://www.readbyqxmd.com/read/28842537/molecular-determinants-of-the-matrix-thickening-of-a-dual-species-pseudomonas-aeruginosa-and-enterococcus-faecalis-biofilm
#15
Keehoon Lee, Kang-Mu Lee, Donggeun Kim, Sang Sun Yoon
Biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ECMs). Clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, after introduction of the first biofilm infection model by J.W. Costerton and his colleagues (1). However, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. Pseudomonas aeruginosa and Enterococcus faecalis are two frequently identified bacterial species in biofilm infections; nevertheless, interactions between these two species, especially during biofilm growth, are not clearly understood...
August 25, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28831065/reactive-oxygen-species-mediated-switching-expression-of-mmp-3-in-stromal-fibroblasts-and-cancer-cells-during-prostate-cancer-progression
#16
Chia-Ling Hsieh, Che-Ming Liu, Hsin-An Chen, Shun-Tai Yang, Katsumi Shigemura, Koichi Kitagawa, Fukashi Yamamichi, Masato Fujisawa, Yun-Ru Liu, Wei-Hua Lee, Kuan-Chou Chen, Chia-Ning Shen, Cheng-Chieh Lin, Leland W K Chung, Shian-Ying Sung
Studies on the aberrant control of extracellular matrices (ECMs) have mainly focused on the role of malignant cells but less on that of stromal fibroblasts during cancer development. Herein, by using paired normal and prostate cancer-associated stromal fibroblasts (CAFs) derived from a coculture cell model and clinical patient samples, we demonstrated that although CAFs promoted prostate cancer growth, matrix metalloproteinase-3 (MMP-3) was lower in CAFs but elevated in prostate cancer cells relative to their normal counterparts...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28827814/fibromodulin-deficiency-reduces-collagen-structural-network-but-not-glycosaminoglycan-content-in-a-syngeneic-model-of-colon-carcinoma
#17
P Olof Olsson, Sebastian Kalamajski, Marco Maccarana, Åke Oldberg, Kristofer Rubin
Tumor barrier function in carcinoma represents a major challenge to treatment and is therefore an attractive target for increasing drug delivery. Variables related to tumor barrier include aberrant blood vessels, high interstitial fluid pressure, and the composition and structure of the extracellular matrix. One of the proteins associated with dense extracellular matrices is fibromodulin, a collagen fibrillogenesis modulator expressed in tumor stroma but scarce in normal loose connective tissues. Here, we investigated the effects of fibromodulin on stroma ECM in a syngeneic murine colon carcinoma model...
2017: PloS One
https://www.readbyqxmd.com/read/28816316/hydrogel-scaffolds-for-differentiation-of-adipose-derived-stem-cells
#18
REVIEW
Qiutong Huang, Yijie Zou, Maria C Arno, Shuai Chen, Tao Wang, Jingyi Gao, Andrew P Dove, Jianzhong Du
Natural extracellular matrices (ECMs) have been widely used as a support for the adhesion, migration, differentiation, and proliferation of adipose-derived stem cells (ADSCs). However, poor mechanical behavior and unpredictable biodegradation properties of natural ECMs considerably limit their potential for bioapplications and raise the need for different, synthetic scaffolds. Hydrogels are regarded as the most promising alternative materials as a consequence of their excellent swelling properties and their resemblance to soft tissues...
August 17, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28816087/small-diameter-vascular-graft-with-fibroblast-cells-and-electrospun-poly-l-lactide-co-%C3%AE%C2%B5-caprolactone-scaffolds-cell-matrix-engineering
#19
Bong Seok Jang, Ja Young Cheon, Soo Hyun Kim, Won Ho Park
Electrospun scaffolds have been widely used in tissue engineering due to their similar structure to native extracellular matrices (ECM). However, one of the obstacles limiting the application of electrospun scaffolds for tissue engineering is the nano-sized pores, which inhibit cell infiltration into the scaffolds. To overcome this limitation, we approached to make layers which are consisted of cells onto the electrospun sheet and then tubular structure was constructed by rolling. We called this as 'Cell Matrix Engineering' because the electrospun sheets were combined with the cells to form one matrix...
August 23, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28811169/tissue-engineered-extracellular-matrices-ecms-in-urology-evolution-and-future-directions
#20
REVIEW
N F Davis, E M Cunnane, F J O'Brien, J J Mulvihill, M T Walsh
Autologous gastrointestinal tissue has remained the gold-standard reconstructive biomaterial in urology for >100 years. Mucus-secreting epithelium is associated with lifelong metabolic and neuromechanical complications when implanted into the urinary tract. Therefore, the availability of biocompatible tissue-engineered biomaterials such as extracellular matrix (ECM) scaffolds may provide an attractive alternative for urologists. ECMs are decellularised, biodegradable membranes that have shown promise for repairing defective urinary tract segments in vitro and in vivo by inducing a host-derived tissue remodelling response after implantation...
August 12, 2017: Surgeon: Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
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