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https://www.readbyqxmd.com/read/27921337/extended-spectrum-cephalosporin-resistance-among-clinical-isolates-of-enterobacteriaceae-in-west-norway-during-2006-2013-a-prospective-surveillance-study
#1
Haima Mylvaganam, Helge Kolstad, Rebecca Irene Breistein, Grete Lind, Dag Harald Skutlaberg
Routine surveillance of resistance to broad-spectrum cephalosporins in Enterobacteriaceae and phenotypic identification of underlying mechanisms using a simple strategy was commenced in 2006 at our laboratory, serving West Norway. This report focuses on the results until 2013. The classical plasmid-mediated extended spectrum beta-lactamase (ESBLA ) among clinically relevant Escherichia coli isolates showed an increase from 0.6% to 4.3% during the surveillance period, while prevalence for other mechanisms remained stable, below 0...
December 6, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27919902/genetic-environment-of-the-blakpc-2-gene-in-klebsiella-pneumoniae-isolate-which-may-have-been-imported-to-russia-from-southeast-asia
#2
Vladimir Ageevets, Julia Sopova, Irina Lazareva, Maya Malakhova, Elena Ilina, Elena Kostryukova, Vladislav Babenko, Alessandra Carattoli, Yuri Lobzin, Alexander Uskov, Sergey Sidorenko
The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA, mrx, mphR), and a fragment of blaTEM with unique polymorphisms...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919898/a-prolonged-outbreak-of-kpc-3-producing-enterobacter-cloacae-and-klebsiella-pneumoniae-driven-by-multiple-mechanisms-of-resistance-transmission-at-a-large-academic-burn-center
#3
Hajime Kanamori, Christian M Parobek, Jonathan J Juliano, David van Duin, Bruce A Cairns, David J Weber, William A Rutala
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter cloacae have been recently recognized in the United States. Whole-genome sequencing (WGS) has become a useful tool for analysis of outbreaks and for determining transmission networks of multidrug-resistant organisms in healthcare settings, including carbapenem-resistant Enterobacteriaceae (CRE). We experienced a prolonged outbreak of CRE of E. cloacae and K. pneumoniae over a three-year period at a large academic burn center despite rigorous infection control measures...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919742/refugees-and-antimicrobial-resistance-a-systematic-review
#4
REVIEW
Allard Willem de Smalen, Hatem Ghorab, Moataz Abd El Ghany, Grant A Hill-Cawthorne
BACKGROUND: There is a large increase in the numbers of refugees and asylum seekers worldwide and a lack of data on the carriage of antimicrobial resistance in refugee/asylum seeking groups. METHODS: This article aims to identify the impact of refugees and asylum seekers on the acquisition and transmission of antimicrobial resistance (AMR) through a literature search. The databases Embase, Medline, Pubmed, and Web of Science Core Collection were utilised and covered all articles before the 1st of October 2016...
December 2, 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27919513/-rapid-test-for-detection-of-susceptibility-to-cefotaxime-in-enterobacteriaceae
#5
Gemma Jiménez-Guerra, Yannik Hoyos-Mallecot, Javier Rodríguez-Granger, José María Navarro-Marí, José Gutiérrez-Fernández
In this work an "in house" rapid test based on the change in pH that is due to hydrolysis for detecting Enterobacteriaceae susceptible to cefotaxime is evaluated. The strains of Enterobacteriaceae from 1947 urine cultures were assessed using MicroScan panels and the "in house" test. This rapid test includes red phenol solution and cefotaxime. Using MicroScan panels, 499 Enterobacteriaceae isolates were evaluated, which included 27 isolates of Escherichia coli producing extended-spectrum beta-lactamases (ESBL), 16 isolates of Klebsiella pneumoniae ESBL and 1 isolate of Klebsiella oxytoca ESBL...
December 2, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27919287/chemical-profiling-antimicrobial-and-insecticidal-evaluations-of-polygonum-hydropiper-l
#6
Muhammad Ayaz, Muhammad Junaid, Farhat Ullah, Abdul Sadiq, Muhammad Ovais, Waqar Ahmad, Sajjad Ahmad, Anwar Zeb
BACKGROUND: The emergence of multidrug resistant (MDR) pathogens is of great concern to the global health community. Our ability to effectively treat diseases is based on the discovery of potent drugs for the treatment of these challenging diseases. Traditional medicines are one of the major sources for the discovery of safe, effective and economical drug candidates. In order to validate its antibacterial, antifungal and insecticidal potentials with respect to traditional uses, we have screened for the first time Polygonum hydropiper against pathogenic bacterial, fungal strains and a variety of insects...
December 5, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27917834/the-crystal-structure-of-the-endoglucanase-cel10-a-family-8-glycosyl-hydrolase-from-klebsiella-pneumoniae
#7
Ayman Attigani, Lifang Sun, Qing Wang, Yadan Liu, Dingping Bai, Shengping Li, Xiaohong Huang
Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain of Klebsiella pneumoniae isolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyze p-nitrophenol β-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27917346/neonatal-bacterial-meningitis-in-tikur-anbessa-specialized-hospital-ethiopia-a-10-year-retrospective-review
#8
Melese Abate Reta, Tamrat Abebe Zeleke
BACKGROUND: Bacterial meningitis is still a major public health threat in developing countries. It is an overwhelming infection with a high morbidity and mortality rate, especially in neonates. The aim of this study was to determine the prevalence and etiological agents that cause bacterial neonatal meningitis at Tikur Anbessa Specialized Hospital (TASH). METHODS: This is a retrospective analysis of 1189 cerebrospinal fluid (CSF) specimens submitted to the bacteriology laboratory of TASH for culture from 2001 to 2010...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27917275/synergistic-effect-of-thymbra-spicata-l-extracts-with-antibiotics-against-multidrug-resistant-staphylococcus-aureus-and-klebsiella-pneumoniae-strains
#9
Mohammad F Haroun, Rawaa S Al-Kayali
OBJECTIVES: To evaluate the in vitro interaction between different extracts of Thymbra spicata L. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. This study was performed against multidrug-resistant strains of Staphylococcus aureus and Klebsiella pneumoniae. MATERIALS AND METHODS: Evaluation of antibacterial activity and synergy interaction between plant extracts and antimicrobial agents was carried out using checkerboard microdilution...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27916936/capsule-targeting-depolymerase-derived-from-klebsiella-kp36-phage-as-a-tool-for-the-development-of-anti-virulent-strategy
#10
Grażyna Majkowska-Skrobek, Agnieszka Łątka, Rita Berisio, Barbara Maciejewska, Flavia Squeglia, Maria Romano, Rob Lavigne, Carsten Struve, Zuzanna Drulis-Kawa
The rise of antibiotic-resistant Klebsiella pneumoniae, a leading nosocomial pathogen, prompts the need for alternative therapies. We have identified and characterized a novel depolymerase enzyme encoded by Klebsiella phage KP36 (depoKP36), from the Siphoviridae family. To gain insights into the catalytic and structural features of depoKP36, we have recombinantly produced this protein of 93.4 kDa and showed that it is able to hydrolyze a crude exopolysaccharide of a K. pneumoniae host. Using in vitro and in vivo assays, we found that depoKP36 was also effective against a native capsule of clinical K...
December 1, 2016: Viruses
https://www.readbyqxmd.com/read/27916031/-experience-of-the-selection-and-maintainence-for-donor-lung-62-cases-attached
#11
G L Peng, X Xu, D X Huang, B Wei, D Xiao, W Wang, Y D Wang, J X He
Objective: To summarize the experience of selection and maintainence for the lungs from donation. Methods: From January 2015 to June 2016, 62 cases had been evaluated by Department of Thoracic Surgery, First Affiliated Hospital of Guangzhou Medical University. All the clinic data had been collected to evaluate the donor lungs, including the basic information, physical examination, results of radiology and laboratory, the bronchoscope, etc. All the satisfactory donors had been harvested and transplanted to the receipient and then follow up...
December 1, 2016: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/27914962/efflux-mediated-colistin-resistance-in-diverse-clones-of-klebsiella-pneumoniae-from-aquatic-environment
#12
Santosh Kumar Singh, Mitali Mishra, Minu Sahoo, Shashank Patole, Harapriya Mohapatra
The peptide drug colistin is commonly used to treat carbapenem resistant gram negative bacterial infections. In the present study, we report efflux mediated colistin resistance in multidrug resistant Klebsiella pneumoniae isolates belonging to ST200 and ST1296, isolated from a fresh water environment. The isolates exhibited intermediate resistance to human serum, possessed Type 1 fimbriae and harbored blaSHV-34 and blaTEM-1 genes. Our results highlight the evolving nature of these clones in the country. These observations emphasize the need for judicious usage of antibiotics to prevent the imminent danger of losing out on currently available therapeutic options...
November 30, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27914678/primary-liver-abscess-caused-by-klebsiella-pneumoniae-an-increasingly-common-entity
#13
Clara Pañella, Daniel Flores-Pereyra, Lourdes Hernández-Martínez, Fernando Burdío, Luís Grande, Ignasi Poves
No abstract text is available yet for this article.
November 30, 2016: Gastroenterología y Hepatología
https://www.readbyqxmd.com/read/27910828/biofilm-prevention-of-gram-negative-bacterial-pathogens-involved-in-periprosthetic-infection-by-antibiotic-loaded-calcium-sulfate-beads-in-vitro
#14
Robert P Howlin, Christopher Winnard, Connor J Frapwell, Jeremy S Webb, John J Cooper, Sean S Aiken, Paul Stoodley
Biofilm formation represents a key stage in the pathogenesis of prosthetic infections (PIs). More tolerant to antibiotics than their planktonic counterparts, biofilm bacteria are difficult to eradicate using conventional therapeutic regimes. A common approach in PI management is the adjunctive use of localised antibiotics in addition to systemic administration in an attempt to protect the implant from colonisation by infiltrating bacteria. This study evaluates the antibacterial and antibiofilm efficacy of antibiotic-loaded dissolvable calcium sulphate, previously shown to be effective against key gram-positive pathogens, against gram-negative species important in the establishment of chronic infection in PIs...
December 2, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27909821/previous-bloodstream-infections-due-to-other-pathogens-as-predictors-of-carbapenem-resistant-klebsiella-pneumoniae-bacteraemia-in-colonized-patients-results-from-a-retrospective-multicentre-study
#15
D R Giacobbe, V Del Bono, P Bruzzi, S Corcione, M Giannella, A Marchese, L Magnasco, A E Maraolo, N Pagani, C Saffioti, S Ambretti, C S Cardellino, E Coppo, F G De Rosa, P Viale, C Viscoli
INTRODUCTION: the purpose of this retrospective multicenter study was to assess whether the risk of developing bloodstream infections (BSI) due to carbapenem-resistant Klebsiella pneumoniae (CRKP) in colonized patients is influenced by the occurrence of BSI due to other pathogens. METHODS: from January 2012 to March 2014, all patients with at least one rectal swab positive for CRKP and at least 30 days of previous hospital stay were included in the study. The primary outcome measure was CRKP BSI, defined as a time-to-event endpoint...
December 1, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27909624/for-reliable-urine-cultures-in-the-detection-of-complicated-urinary-tract-infection-do-we-use-urine-specimens-obtained-with-urethral-catheter-or-a-nephrostomy-tube
#16
Gülay Dede, Özcan Deveci, Onur Dede, Mazhar Utanğac, Mansur Dağgulli, Necmettin Penbegül, Namık Kemal Hatipoğlu
OBJECTIVE: The aim of this study was to compare the results of urine cultures obtained either from urethral, and percutaneous nephrostomy (PCN) catheters. MATERIALS AND METHODS: This study included 328 consecutive patients that underwent PCN at our institution with complicated urinary tract infections (UTIs) between July 2010 and April 2015. Results of urine cultures obtained from the urethral and nephrostomy catheters were compared. RESULTS: This study included 152 male and 176 female patients...
December 2016: Turkish Journal of Urology
https://www.readbyqxmd.com/read/27906145/nitrobacter-sp-extract-mediated-biosynthesis-of-ag2o-nps-with-excellent-antioxidant-and-antibacterial-potential-for-biomedical-application
#17
Gopalu Karunakaran, Matheswaran Jagathambal, Alexander Gusev, Nguyen Van Minh, Evgeny Kolesnikov, Arup Ratan Mandal, Denis Kuznetsov
In this study, extracellular extract of plant growth promoting bacterium, Nitrobacter sp. is used for the bioconversion of AgNO3 (silver nitrate) into Ag2O (silver oxide nanoparticles). It is an easy, ecofriendly and single step method for Ag2O NPs synthesis. The bio-synthesized nanoparticles were characterized using different techniques. UV-Vis results showed the maximum absorbance around 450 nm. XRD result shows the particles to have faced centered cubic (fcc) crystalline nature. FTIR analysis reveals the functional groups that are involved in bioconversion such as C-N, N-H and C=O...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27906139/myxobacteria-mediated-synthesis-of-silver-nanoparticles-and-their-impregnation-in-wrapping-paper-used-for-enhancing-shelf-life-of-apples
#18
Swati Bhople, Swapnil Gaikwad, Swapna Deshmukh, Shital Bonde, Aniket Gade, Sanjib Sen, Anna Brezinska, Hanna Dahm, Mahendra Rai
The authors report Myxobacteria virescens (M. virescens) mediated synthesis of silver nanoparticles (AgNPs) and its efficacy against Staphylococcus aureus (ATCC-33591), Salmonella typhi (ATCC-51812), Escherichia coli (E. coli) (ATCC-14948), Klebsiella pneumoniae (MTCC-4030) and Pseudomonas aeruginosa (MTCC-4673). The organism exhibiting resistance to various antibiotics showed remarkable sensitivity, when used in combination of antibiotics and AgNPs. Antimicrobial property of AgNPs is playing a significant role in medicine and food storage...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27906136/rapid-synthesis-of-silver-nanoparticles-by-pseudomonas-stutzeri-isolated-from-textile-soil-under-optimised-conditions-and-evaluation-of-their-antimicrobial-and-cytotoxicity-properties
#19
Nishant Rajora, Sanket Kaushik, Anupam Jyoti, Shanker L Kothari
Present study utilised textile soil isolated bacterium Pseudomonas stutzeri to synthesise extracellular silver nanoparticles (AgNPs) under optimised conditions. The synthesised AgNPs were characterised using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Optimisation showed AgNPs synthesis within 8 h using 2mM Ag nitrate at pH9, temperature 80°C and maximum absorbance toward 400 nm. TEM analysis revealed spherical shape AgNPs and reduction in size upto 8 nm was observed under optimised conditions...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27905092/broad-spectrum-of-antimicrobial-properties-of-commercial-wines-from-different-vitis-vinifera-l-varieties
#20
Aleksandra Radovanović, Biljana Arsić, Vladimir Radovanović, Branimir Jovančičević, Vesna Nikolić
Fifteen commercial wines produced from international and autochthonic varieties of Vitis vinifera L. cultivation of different Balkan winegrowing subregions were studied for their antimicrobial activity against six Gram-positive (Clostridium perfringens, Bacillus subtilis, Staphylococcus aureus, Listeria inocua, Sarcina lutea and Micrococcus flavus) and six Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella sonnei, Klebsiella pneumonia and Proteus vulgaris) bacteria. The concentrations and types of phenolic compounds responsible for antibacterial activity in wines were investigated by HPLC and spectroscopic methods...
January 2017: World Journal of Microbiology & Biotechnology
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