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https://www.readbyqxmd.com/read/29054092/protein-mimicking-nanoparticles-for-the-reproduction-of-transient-protein-receptor-interactions
#1
Jonghwan Lee
One of the major concerns in target identification has been the need for new methods to detect target molecules in the native cellular environment. In conventional target identification, affinity-based pull down has been conducted using cell lysates. However, interactions in cell lysate do not reflect real endogenous interactions in living cells, and can produce false-positive or false-negative results. This study aimed to develop a new method of target protein identification in living cells. Targeting probes were conjugated onto magnetic nanoparticles (MNPs)...
October 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29054021/qprtase-modified-n-doped-carbon-quantum-dots-a-fluorescent-bioprobe-for-selective-detection-of-neurotoxin-quinolinic-acid-in-human-serum
#2
Ranjana Singh, Sunayana Kashayap, Vimal Singh, Arvind M Kayastha, Hirdyesh Mishra, Preeti Suman Saxena, Anchal Srivastava, Ranjan K Singh
Highly fluorescent nitrogen doped carbon quantum dots (NCQDs) were synthesized using microwave assisted green method. It was characterized by Transmission Electron Microscopy (TEM), FTIR, UV-Visible absorption and Photoluminiscence (PL) techniques. The NCQDs were immobilized with an enzyme named quinolinate phoshphoribosyl transferase (QPRTase). The NCQDs immobilized by QPRTase was used as a fluorescent bioprobe for the selective detection of endogenous neurotoxin quinolinic acid (QA) whose elevated level in serum is marker of many neurological disorders such as Alzheimer's, Huntington's and HIV associated dementia (HAD) as well as deficiency of vitamin B6...
October 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29053991/molecular-mechanisms-of-development-of-the-human-fetal-female-reproductive-tract
#3
Gerald R Cunha, Takeshi Kurita, Mei Cao, Joel Shen, Stanley Robboy, Laurence Baskin
Human female reproductive tract development rests mostly upon hematoxilyn and eosin stained sections despite recent advances on molecular mechanisms in mouse studies. We report application of immunohistochemical methods to explore the ontogeny of epithelial and mesenchymal differentiation markers (keratins, homobox proteins, steroid receptors), transcription factors and signaling molecules (TP63 and RUNX1) during human female reproductive tract development. Keratins 6, 7, 8, 10, 14 and 19 (KRT6, KRT7, KRT8, KRT10, KRT14, KRT19) were expressed in a temporally and spatially dynamic fashion...
July 29, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29053814/effects-of-canola-meal-source-on-the-standardized-ileal-digestible-amino-acids-and-apparent-metabolizable-energy-contents-for-broiler-chickens
#4
D I Adewole, A Rogiewicz, B Dyck, B A Slominski
Two studies were conducted to determine the effect of canola meal source on the standardized ileal digestible amino acids (AA) and nitrogen-corrected apparent metabolizable energy (AMEn) contents of canola meal (CM) from 6 processing plants in Canada. Two plants provided CM in both mash and pellet forms, while the other 4 provided CM in mash form only, giving a total of 8 samples. In the standardized ileal digestibility (SID) assay, 280 birds were housed 7 per cage and fed a commercial starter diet from 1 to 14 d of age followed by the test diets from 15 to 21 d...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053779/cell-based-therapeutic-strategies-for-multiple-sclerosis
#5
Neil J Scolding, Marcelo Pasquini, Stephen C Reingold, Jeffrey A Cohen
The availability of multiple disease-modifying medications with regulatory approval to treat multiple sclerosis illustrates the substantial progress made in therapy of the disease. However, all are only partially effective in preventing inflammatory tissue damage in the central nervous system and none directly promotes repair. Cell-based therapies, including immunoablation followed by autologous haematopoietic stem cell transplantation, mesenchymal and related stem cell transplantation, pharmacologic manipulation of endogenous stem cells to enhance their reparative capabilities, and transplantation of oligodendrocyte progenitor cells, have generated substantial interest as novel therapeutic strategies for immune modulation, neuroprotection, or repair of the damaged central nervous system in multiple sclerosis...
July 21, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29053584/lomefloxacin-induces-oxidative-stress-and-apoptosis-in-colo829-melanoma-cells
#6
Artur Beberok, Dorota Wrześniok, Martyna Szlachta, Jakub Rok, Zuzanna Rzepka, Michalina Respondek, Ewa Buszman
Although some fluoroquinolones have been found to exert anti-tumor activity, studies on the effect of these drugs on melanoma cells are relatively rare. The aim of this study was to examine the effect of lomefloxacin on cell viability, reactive oxygen species production, redox balance, cell cycle distribution, DNA fragmentation, and apoptosis in COLO829 melanoma cells. Lomefloxacin decreases the cell viability in a dose- and time-dependent manner. For COLO829 cells treated with the drug for 24, 48, and 72 h, the values of IC50 were found to be 0...
October 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29053406/super-resolution-nanoscopy-imaging-applied-to-dna-double-strand-breaks
#7
Sofia D'Abrantes, Sarah Gratton, Pamela Reynolds, Verena Kriechbaumer, Joseph McKenna, Stephen Barnard, Dave Clarke, Stanley W Botchway
Genomic deoxyribonucleic acid (DNA) is continuously being damaged by endogenous processes such as metabolism or by exogenous events such as radiation. The specific phosphorylation of histone H2AX on serine residue 139, described as γ-H2AX, is an excellent indicator or marker of DNA double-strand breaks (DSBs). The yield of γ-H2AX (foci) is shown to have some correlation with the dose of radiation or other DSB-causing agents. However, there is some discrepancy in the DNA DSB foci yield among imaging and other methods such as gel electrophoresis...
October 20, 2017: Radiation Research
https://www.readbyqxmd.com/read/29053249/holistic-lipidomics-of-the-human-gut-phenotype-using-validated-ultra-high-performance-liquid-chromatography-coupled-to-hybrid-orbitrap-mass-spectrometry
#8
Lieven Van Meulebroek, Ellen De Paepe, Vicky Vercruysse, Beata Pomian, Simon Bos, Bruno Lapauw, Lynn Vanhaecke
As lipids are assigned a plethora of biological functions, it is evident that a dysregulated lipid metabolism signifies a key element in many pathological conditions. With this rationale, this study presents a validated lipidomics platform to map the fecal lipidome, which integrates unique information about the host-gut microbiome interactions, gastrointestinal functionality, and dietary pattern. This particular method accomplished coverage across all eight lipid categories; the fatty acyls, glycerolipids, phosphoglycerolipids, polyketides, prenols, saccharolipids, sphingolipids, and sterols...
October 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29053187/xist-promotes-gastric-cancer-gc-progression-through-tgf-%C3%AE-1-via-targeting-mir-185
#9
Quan Zhang, Baiyu Chen, Ping Liu, Jing Yang
LncRNAs and microRNAs can play significant roles in various cancers, including gastric cancer (GC). In our study, we investigated the role of lncRNA XIST in GC. We observed that XIST was increased in MGC803 and BGC823 cells compared to human normal gastric epithelial GES-1 cells. It was also shown that miR-185 was decreased in GC cell lines. Silencing XIST can inhibit the growth of GC cells and bioinformatics analysis was performed to confirm the correlation between XIST and miR-185. Interestingly, a negative correlation was indicated between XIST and miR-185 in GC cells...
October 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29053142/resolution-of-inflammation-and-sepsis-survival-are-improved-by-dietary-%C3%AE-3-fatty-acids
#10
Andreas Körner, Martin Schlegel, Julia Theurer, Hannes Frohnmeyer, Michael Adolph, Marieke Heijink, Martin Giera, Peter Rosenberger, Valbona Mirakaj
Critical conditions such as sepsis following infection or traumatic injury disturb the complex state of homeostasis that may lead to uncontrolled inflammation resulting in organ failure, shock and death. They are associated with endogenous mediators that control the onset of acute inflammatory response, but the central problem remains the complete resolution of inflammation. Omega-3 enriched lipid emulsions (Ω-3(+) LEs) were used in experimental studies and clinical trials to establish homeostasis, yet with little understanding about their role on the resolution of inflammation and tissue regeneration...
October 20, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29053124/-the-efficacy-of-cerebrolysin-in-the-treatment-of-autism-spectrum-disorders
#11
L S Chutko, E A Yakovenko, S Yu Surushkina, E M Kryukova, S V Palaieva
AIM: To evaluate clinical and neurophysiological changes in children with different variants of autism spectrum disorders during treatment with cerebrolysin. MATERIAL AND METHODS: Forty-three children with autism spectrum disorders, aged 4-6 years, were included in the study. To assess the degree of autism, the quantitative scale of assessing the severity of child autism CARS ('Childhood Autism Rating Scale') was used. A comparative analysis of the clinical picture of disease and data of the electroencephalographic examination in endogenous and exogenous (organic) variant of autism was made...
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/29053080/aerosolizing-lipid-dispersions-enables-antibiotic-transport-across-mimics-of-the-lung-airway-surface-even-in-the-presence-of-pre-existing-lipid-monolayers
#12
Steven V Iasella, Amy Z Stetten, Timothy E Corcoran, Stephen Garoff, Todd M Przybycien, Robert D Tilton
BACKGROUND: Secondary lung infections are the primary cause of morbidity associated with cystic fibrosis lung disease. Aerosolized antibiotic inhalation is potentially advantageous but has limited effectiveness due to altered airway aerodynamics and deposition patterns that limit drug access to infected regions. One potential strategy to better reach infected areas is to formulate aerosols with surfactants that induce surface tension gradients and drive postdeposition drug dispersal via Marangoni transport along the airway surface liquid (ASL)...
October 20, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/29052983/in-vivo-biochemistry-single-cell-dynamics-of-cyclic-di-gmp-in-e-coli-in-response-to-zinc-overload
#13
Jongchan Yeo, Andrew B Dippel, Xin C Wang, Ming C Hammond
Intracellular signaling enzymes drive critical changes in cellular physiology and gene expression, but their endogenous activities in vivo remain highly challenging to study in real-time and for individual cells. Here we show that flow cytometry can be performed in complex media to monitor single-cell population distributions and dynamics of cyclic di-GMP signaling, which controls the bacterial colonization program. These in vivo biochemistry experiments are enabled by our second-generation RNA-based fluorescent (RBF) biosensors, which exhibit high fluorescence turn-on in response to cyclic di-GMP...
October 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/29052767/acute-regulation-of-multidrug-resistance-associated-protein-2-localization-and-activity-by-camp-and-estradiol-17%C3%AE-d-glucuronide-in-rat-intestine-and-caco-2-cells
#14
Guillermo Nicolás Tocchetti, Agostina Arias, Maite Rocío Arana, Juan Pablo Rigalli, Camila Juliana Domínguez, Felipe Zecchinati, María Laura Ruiz, Silvina Stella Maris Villanueva, Aldo Domingo Mottino
Multidrug resistance-associated protein 2 (MRP2) is an ATP-dependent transporter expressed at the brush border membrane of the enterocyte that confers protection against absorption of toxicants from foods or bile. Acute, short-term regulation of intestinal MRP2 activity involving changes in its apical membrane localization was poorly explored. We evaluated the effects of dibutyryl-cAMP (db-cAMP), a permeable analog of cAMP, and estradiol-17β-D-glucuronide (E217G), an endogenous derivative of estradiol, on MRP2 localization and activity using isolated rat intestinal sacs and Caco-2 cells, a model of human intestinal epithelium...
October 20, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29052760/impact-of-plasmid-architecture-on-stability-and-yegfp3-reporter-gene-expression-in-a-set-of-isomeric-multicopy-vectors-in-yeast
#15
Ruben Hohnholz, Kim Julia Pohlmann, Tilman Achstetter
Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. Considering that rich medium favors growth rate and, simultaneously, is less expensive than selective medium, enhancing stability in non-selective medium is extremely desirable. In this study, we changed the architecture of a multicopy model expression plasmid, creating six isoforms (same size, same DNA content but different positions and orientations of the expression block) and studied mitotic stability, copy number, as well as reporter yEGFP3 expression between isoforms...
October 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29052539/engineering-diagnostic-and-therapeutic-gut-bacteria
#16
Brian P Landry, Jeffrey J Tabor
Genetically engineered bacteria have the potential to diagnose and treat a wide range of diseases linked to the gastrointestinal tract, or gut. Such engineered microbes will be less expensive and invasive than current diagnostics and more effective and safe than current therapeutics. Recent advances in synthetic biology have dramatically improved the reliability with which bacteria can be engineered with the sensors, genetic circuits, and output (actuator) genes necessary for diagnostic and therapeutic functions...
October 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/29052517/constitutive-expression-of-osdof4-encoding-a-c2-c2-zinc-finger-transcription-factor-confesses-its-distinct-flowering-effects-under-long-and-short-day-photoperiods-in-rice-oryza-sativa-l
#17
Qi Wu, Xue Liu, Dedong Yin, Hua Yuan, Qi Xie, Xianfeng Zhao, Xiaobing Li, Lihuang Zhu, Shigui Li, Dayong Li
BACKGROUND: Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors which contain a conserved C2-C2-type zinc finger domain, are involved in many fundamental processes. In the Arabidopsis photoperiod response pathway, CDF (CYCLING DOF FACTOR) proteins have a primary role as acting via transcriptional repression of the direct FLOWERING LOCUS T (FT) activator CONSTANS (CO). Our previous study indicated that one of CDF homologs, OsDOf12, was involved in photoperiodic flowering...
October 19, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29052471/a-review-about-lycopene-induced-nuclear-hormone-receptor-signalling-in-inflammation-and-lipid-metabolism-via-still-unknown-endogenous-apo-10%C3%A2-lycopenoids
#18
Catherine Caris-Veyrat, Ada L Garcia, Eric Reynaud, Renata Lucas, Gamze Aydemir, Ralph Rühl
Lycopene is the red pigment in tomatoes and tomato products and is an important dietary carotenoid found in the human organism. Lycopene-isomers, oxidative lycopene metabolites and apo-lycopenoids are found in the food matrix. Lycopene intake derived from tomato consumption is associated with alteration of lipid metabolism and a lower incidence of cardiovascular diseases (CVD). Lycopene is mainly described as a potent antioxidant but novel studies are shifting towards its metabolites and their capacity to mediate nuclear receptor signalling...
October 20, 2017: International Journal for Vitamin and Nutrition Research. Journal International de Vitaminologie et de Nutrition
https://www.readbyqxmd.com/read/29052403/-serum-metabonomics-in-mice-infected-with-mycoplasma-pneumoniae-by-uplc-q-tof-ms
#19
Wen-Feng Wei, Yan-Tao Chu, Ye Liu, Jin-Hai Huo, Wei-Ming Wang
Ultra high performance liquid chromatography coupled with tandem quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS) was applied to metabonomics study in BALB/c mice infected with mycoplasma pneumoniae(MP) to analyze the changes in serum endogenous metabolites, identify potential biomarkers associated with mycoplasma pneumoniae pneumonia(MPP), analyze the metabolic pathway and explore the pathogenic mechanism of MPP. The BALB/c mice were inoculated with MP by repeated intranasal infectious routes to establish MPP models, and the results of the lung tissue biopsy, IgM and mycoplasma nucleic acid content determination showed that the models of MP in BALB/c mice were successfully established...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052256/structure-and-function-of-human-tyrosinase-and-tyrosinase-related-proteins
#20
Xuelei Lai, Harry J Wichers, Montserrat Soler-Lopez, Bauke W Dijkstra
Melanin is the main pigment responsible for the colour of human skin, hair and eye. Its biosynthesis requires three melanogenic enzymes, tyrosinase (TYR), and the tyrosinase-related proteins TYRP1 and TYRP2. The difficulty of isolating pure and homogeneous proteins from endogenous sources has hampered their study, and resulted in many contradictory findings regarding their physiological functions. In this review, we summarize recent advances on the structure and function of TYR and TYRPs by virtue of the crystal structure of human TYRP1, which is the first available structure of a mammalian melanogenic enzyme...
October 20, 2017: Chemistry: a European Journal
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