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https://www.readbyqxmd.com/read/28109156/-cloning-and-sequence-analysis-of-variable-region-genes-of-mouse-anti-human-s100a9-monoclonal-antibody
#1
Min Yan, Danyang Mi, Xiangdong Sun, Zhenqiang Zhang, Huahui Zeng, Wendi Liu
Objective To clone and analyze variable region gene sequences of mouse monoclonal antibody against human S100A9 (anti-human S100A9 mAb). Methods Total RNA was extracted from the hybridoma cell line (IID4B10) secreting mouse anti-human S100A9 mAb. Furthermore, the heavy chain variable region (VH) and light chain variable region (VL) genes were amplified using reverse transcription-polymerase chain reaction (RT-PCR). After the cloning and sequencing of the VH and VL genes, the sequence analyses were performed by BLAST program...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109062/fibroblasts-as-maestros-orchestrating-tissue-regeneration
#2
REVIEW
Raquel Costa-Almeida, Raquel Soares, Pedro L Granja
Fibroblasts constitute a dynamic and versatile population of cells of mesenchymal origin, implicated in both regenerative strategies and pathological conditions. Despite being frequently associated to disease development, particularly through the establishment of fibrotic tissue, fibroblasts hold great potential for tissue engineering and regenerative medicine applications. They are responsible for synthesizing and depositing extracellular matrix components, allowing other cells to settle and migrate along a three-dimensional support and thereby generating an organ-specific architecture...
January 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28108960/dual-acting-biofunctionalised-scaffolds-for-applications-in-regenerative-medicine
#3
Camilo Chaves, Chuanyu Gao, Jerome Hunckler, Moustafa Elsawy, Josette Legagneux, Gilles Renault, Alain Charles Masquelet, Achala de Mel
Off the shelf scaffolds for replacing ultra-small diameter vascular grafts are valuable for reconstruction of diseased or damaged vessels. The limitations for such grafts include optimal handling with ready availability of varied lengths of grafts, graft patency with the ability to replace the function of active cellular mechanisms and adequate mechanical properties to maintain physicochemical function. We used a well-established, solvent casting method for potential tissue replacement scaffold fabrication with incorporated bioactive molecules, which we have previously explored to confer haemocompatibility...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#4
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108950/t-cell-receptor-engineered-t-cells-for-cancer-treatment-current-status-and-future-directions
#5
REVIEW
Yu Ping, Chaojun Liu, Yi Zhang
T-cell receptor (TCR)-engineered T cells are a novel option for adoptive cell therapy used for the treatment of several advanced forms of cancer. Work using TCR-engineered T cells began more than two decades ago, with numerous preclinical studies showing that such cells could mediate tumor lysis and eradication. The success of these trials provided the foundation for clinical trials, including recent clinical successes using TCR-engineered T cells to target New York esophageal squamous cell carcinoma (NY-ESO-1)...
January 20, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28108925/soil-compaction-effects-on-soil-health-and-cropproductivity-an-overview
#6
Adnan Noor Shah, Mohsin Tanveer, Babar Shahzad, Guozheng Yang, Shah Fahad, Saif Ali, Muhammad Adnan Bukhari, Shahbaz Atta Tung, Abdul Hafeez, Biangkham Souliyanonh
Soil compaction causes substantial reduction in agriculture productivity and has always been of great distress for farmers. Intensive agriculture seems to be more crucial in causing compaction. High mechanical load, less crop diversification, intensive grazing, and irrigation methods lead to soil compaction. It is further exasperated when these factors are accompanied with low organic matter, animal trampling, engine vibrations, and tillage at high moisture contents. Soil compaction increases soil bulk density and soil strength, while decreases porosity, aggregate stability index, soil hydraulic conductivity, and nutrient availability, thus reduces soil health...
January 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28108898/bioresorbable-scaffolds-for-coronary-artery-disease
#7
REVIEW
Ashwin Nathan, Taisei Kobayashi, Daniel M Kolansky, Robert L Wilensky, Jay Giri
PURPOSE OF REVIEW: The purpose of this review is to present an overview of the recent evidence regarding the use of bioresorbable scaffolds in percutaneous coronary intervention. RECENT FINDINGS: Bioresorbable scaffolds represent a potentially unique engineering solution to the problems associated with metallic stents. The Absorb everolimus-eluting bioresorbable scaffold has been the most extensively tested of this class and is currently Food and Drug Administration-approved for use in the USA...
January 2017: Current Cardiology Reports
https://www.readbyqxmd.com/read/28108833/soft-tissue-substitutes-in-non-root-coverage-procedures-a-systematic-review-and-meta-analysis
#8
REVIEW
Kristina Bertl, Maximilian Melchard, Nikolaos Pandis, Michael Müller-Kern, Andreas Stavropoulos
OBJECTIVES: The present systematic review compared the effectiveness of soft tissue substitutes (STSs) and autogenous free gingival grafts (FGGs) in non-root-coverage procedures to increase keratinized tissue (KT) width around teeth. MATERIALS AND METHODS: Included studies fulfilled the following main eligibility criteria: (a) preclinical in vivo or human controlled trials using FGG as control, (b) non-root-coverage procedures, and (c) assessment of KT width. Meta-analysis was performed on the gain in KT width (primary outcome variable) and several secondary variables...
January 20, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28108762/enhancement-of-succinate-yield-by-manipulating-nadh-nad-ratio-and-atp-generation
#9
Jiaojiao Li, Yikui Li, Zhiyong Cui, Quanfeng Liang, Qingsheng Qi
We previously engineered Escherichia coli YL104 to efficiently produce succinate from glucose. In this study, we investigated the relationships between the NADH/NAD(+) ratio, ATP level, and overall yield of succinate production by using glucose as the carbon source in YL104. First, the use of sole NADH dehydrogenases increased the overall yield of succinate by 7% and substantially decreased the NADH/NAD(+) ratio. Second, the soluble fumarate reductase from Saccharomyces cerevisiae was overexpressed to manipulate the anaerobic NADH/NAD(+) ratio and ATP level...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28108640/bonds-broken-and-formed-during-the-mixed-linkage-glucan-xyloglucan-endotransglucosylase-reaction-catalysed-by-equisetum-hetero-trans-%C3%AE-glucanase
#10
Thomas J Simmons, Stephen C Fry
Mixed-linkage glucan : xyloglucan endotransglucosylase (MXE) is one of the three activities of the recently characterised hetero-trans-β-glucanase (HTG), which among land-plants is known only from Equisetum species. The biochemical details of the MXE reaction were incompletely understood - details that would promote understanding of MXE's role in vivo and enable its full technological exploitation. We investigated HTG's site of attack on one of its donor substrates, mixed-linkage (1→3),(1→4)-β-D-glucan (MLG), with radioactive oligosaccharides of xyloglucan as acceptor substrate...
January 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28108511/biomarker-based-pet-imaging-of-diffuse-intrinsic-pontine-glioma-in-mouse-models
#11
Susanne Kossatz, Brandon Carney, Melanie E Schweitzer, Giuseppe Carlucci, Vesselin Miloushev, Uday B Maachani, Prajwal Rajappa, Kayvan Keshari, David J Pisapia, Wolfgang A Weber, Mark M Souweidane, Thomas Reiner
Diffuse intrinsic pontine glioma (DIPG) is a childhood brainstem tumor with a universally poor prognosis. Here, we characterize on a positron emission tomography (PET) probe for imaging DIPG in vivo. In human histological tissues, the probes target, poly(ADP)ribose polymerase 1 (PARP1), was highly expressed in DIPG compared to normal brain. PET imaging allowed for the sensitive detection of DIPG in a genetically engineered mouse model (GEMM), and probe uptake correlated to histologically determined tumor infiltration...
January 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/28108294/the-dimensional-reduction-method-for-identification-of-parameters-that-trade-off-due-to-similar-model-roles
#12
Shaun M Davidson, Paul D Docherty, Rua Murray
Parameter identification is an important and widely used process across the field of biomedical engineering. However, it is susceptible to a number of potential difficulties, such as parameter trade-off, causing premature convergence at non-optimal parameter values. The proposed Dimensional Reduction Method (DRM) addresses this issue by iteratively reducing the dimension of hyperplanes where trade off occurs, and running subsequent identification processes within these hyperplanes. The DRM was validated using clinical data to optimize 4 parameters of the widely used Bergman Minimal Model of glucose and insulin kinetics, as well as in-silico data to optimize 5 parameters of the Pulmonary Recruitment (PR) Model...
January 17, 2017: Mathematical Biosciences
https://www.readbyqxmd.com/read/28108246/engineered-cell-lines-for-fish-health-research
#13
Bertrand Collet, Catherine Collins, Katherine Lester
As fish farming continues to increase worldwide, the related research areas of fish disease and immunology are also expanding, aided by the revolution in access to genomic information and molecular technology. The genomes of most fish species of economic importance are now available and annotation based on sequence homology with characterised genomes is underway. However, while useful, functional homology is more difficult to determine, there being a lack of widely distributed and well characterised reagents such as monoclonal antibodies, traditionally used in mammalian studies, to help with confirming functions and cellular interactions of fish molecules...
January 17, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28108235/the-use-of-bacteriophages-to-biocontrol-oral-biofilms
#14
Szymon P Szafrański, Andreas Winkel, Meike Stiesch
Infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. As bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. An innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and can penetrate into biofilms...
January 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28108211/user-centered-design-improves-the-usability-of-drug-drug-interaction-alerts-experimental-comparison-of-interfaces
#15
Daniel R Luna, Daniel A Rizzato Lede, Carlos M Otero, Marcelo R Risk, Fernán González Bernaldo de Quirós
Clinical Decision Support Systems can alert health professionals about drug interactions when they prescribe medications. The Hospital Italiano de Buenos Aires in Argentina developed an electronic health record with drug-drug interaction alerts, using traditional software engineering techniques and requirements. Despite enhancing the drug-drug interaction knowledge database, the alert override rate of this system was very high. We redesigned the alert system using user-centered design (UCD) and participatory design techniques to enhance the drug-drug interaction alert interface...
January 17, 2017: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/28108167/posterior-cruciate-retaining-versus-posterior-stabilized-total-knee-arthroplasty-for-osteoarthritis-with-severe-varus-deformity
#16
Ethem Ayhan Ünkar, Yusuf Öztürkmen, Erhan Şükür, Engin Çarkçı, Murat Mert
OBJECTIVE: The aim of this study was to compare the radiological and functional results of posterior cruciate ligament (PCL) - retaining and posterior-stabilized total knee arthroplasties in patients with severe varus gonarthrosis. METHODS: Medical records of 112 knees of 96 patients who underwent total knee arthroplasty for severe varus (≥15°) were reviewed. PCL-retaining and PCL-stabilizing groups consisted of 58 and 54 knees, respectively. Mean follow-up time was 56...
January 17, 2017: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/28108123/deb-modeling-for-nanotoxicology-microbial-ecology-and-environmental-engineering-comment-on-physics-of-metabolic-organization-by-marko-jusup-et-al
#17
Patricia A Holden
No abstract text is available yet for this article.
January 12, 2017: Physics of Life Reviews
https://www.readbyqxmd.com/read/28108112/the-olfactory-neural-epithelium-as-a-tool-in-neuroscience
#18
Joëlle Lavoie, Patricia Gassó Astorga, Hadar Segal-Gavish, YeeWen Candace Wu, Youjin Chung, Nicola G Cascella, Akira Sawa, Koko Ishizuka
Capturing both dynamic changes (state) and persistent signatures (trait) directly associated with disease at the molecular level is crucial in modern medicine. The olfactory neural epithelium, easily accessible in clinical settings, is a promising surrogate model in translational brain medicine, complementing the limitations in current engineered cell models.
January 17, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28107990/combined-use-of-peptide-ion-and-normalized-delta-scores-to-evaluate-milk-authenticity-by-ion-trap-based-proteomics-coupled-with-error-tolerant-searching
#19
Donatella Nardiello, Anna Natale, Carmen Palermo, Maurizio Quinto, Diego Centonze
A fundamental issue in proteomics is the peptide identification by database searching and the assessment of the goodness of fit between experimental and theoretical data. Despite the different number of ways to measure the quality of search results, the definition of a scoring criterion is still highly desirable in ion-trap based proteomics. Indeed, in order to fully take advantage of a low resolution MS/MS dataset, it is essential to strike a balance between greater information capture and reduced number of incorrect peptide assignments...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107988/quantitative-assay-for-the-detection-screening-and-reactivity-evaluation-of-nanoceria-particles
#20
Ali Othman, Kristen Bear, Silvana Andreescu
Increasing use of engineered nanoparticles (NPs) in many industrial processes and consumer products requires rapid analytical methodologies to reliably detect and screen samples for the presence of NPs. Here, we report the development, analytical characterization and performance evaluation of a colorimetric assay as a comprehensive test for quantitative detection, screening and reactivity evaluation of nanoceria (cerium oxide, CeO2) particles in aqueous environments. We provide a critical discussion of the role of environmental conditions including pH, ionic composition, and presence of humic acid and particle type on the overall performance and sensitivity of this assay...
March 1, 2017: Talanta
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