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https://www.readbyqxmd.com/read/28549374/metabolic-engineering-of-escherichia-coli-to-high-efficient-synthesis-phenylacetic-acid-from-phenylalanine
#1
Lihua Zhang, Qian Liu, Hong Pan, Xun Li, Daoyi Guo
Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28549320/light-emission-miracle-in-the-sea-and-preeminent-applications-of-bioluminescence-in-recent-new-biotechnology
#2
Sana Sharifian, Ahmad Homaei, Roohullah Hemmati, Khosro Khajeh
Bioluminescence is referred to the light emission by a living organism due to a specific biochemical reaction. This interesting feature of the organisms could highly influences behavioral and ecosystem dynamics. Luminescence, mostly observed in marine species, is generally higher in deep-living genera than in benthic or shallow organisms. However, among creatures living in land, fireflies, beetles, springtails and fungi have shown some bioluminescent activities. Classically, the emission of light is catalyzed by luciferase from a substrate...
May 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28549117/engineering-of-recombinant-wisteria-floribunda-agglutinin-specifically-binding-to-galnac%C3%AE-1-4glcnac-lacdinac
#3
Takashi Sato, Hiroaki Tateno, Hiroyuki Kaji, Yasunori Chiba, Tomomi Kubota, Jun Hirabayashi, Hisashi Narimatsu
Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified. Here, we identified the gene encoding WFA, and recombinant WFA was expressed in Escherichia coli and purified. The natural cDNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation...
May 26, 2017: Glycobiology
https://www.readbyqxmd.com/read/28548921/repeatability-testing-of-a-new-hybrid-iii-6-year-old-atd-lower-extremity
#4
Laura C Boucher, Yeonsu Ryu, Yun-Seok Kang, John H Bolte
OBJECTIVE: Vehicle safety is improving, thus decreasing the number of life-threatening injuries and increasing the need for research in other areas of the body. The current child anthropomorphic test device (ATD) does not have the capabilities or instrumentation to measure many of the potential interactions between the lower extremity and the vehicle interior. A prototype Hybrid III 6-year-old ATD lower extremity (ATD-LE) was developed and contains a tibia load cell and a more biofidelic ankle...
May 29, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28548907/evaluation-of-thermal-optical-analysis-method-of-elemental-carbon-for-marine-fuel-exhaust
#5
Maija K Lappi, Jyrki P Ristimäki
The awareness of black carbon (BC) as the second largest anthropogenic contributor in global warming and an ice melting enhancer has increased. Due to prospected increase in shipping especially in the Arctic reliability of BC emissions and their invented amounts from ships is gaining more attention. The International Maritime Organisation (IMO) is actively working towards estimation of quantities and effects of BC especially in the Arctic. IMO has launched work towards constituting a definition for BC and agreeing appropriate methods for its determination from shipping emission sources...
May 26, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28548842/engineering-the-composition-and-structure-of-bimetallic-au-cu-alloy-nanoparticles-in-carbon-nanofibers-self-supported-electrode-materials-for-electrocatalytic-water-splitting
#6
Juan Wang, Han Zhu, Danni Yu, JiaWei Chen, JiaDong Chen, Ming Zhang, LiNa Wang, MingLiang Du
The bimetallic Au-Cu alloy nanoparticles have been constructed in electrospun carbon nanofibers (Au-Cu/CNFs), employing as high efficient hydrogen evolution reaction (HER) electrode. The morphology, structure and composition of bimetallic Au-Cu alloy can be controlled by adjusting the precursor nanofibers through a facile approach. With the increased Cu contents, the Au-Cu alloy have a transition from homogenous AuCu3 alloy phase to Au3Cu phase with Cu shell. The self-supported bimetallic Au-Cu/CNFs hybrid can be directly employed as electrodes materials for water splitting and it showed excellent electrochemical activity, including long-term stability, high exchange current density and low overpotential...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28548831/folate-biofortification-in-hydroponically-cultivated-spinach-by-the-addition-of-phenylalanine
#7
Sho Watanabe, Yuta Ohtani, Yohei Tatsukami, Wataru Aoki, Takashi Amemiya, Yasunori Sukekiyo, Seiichi Kubokawa, Mitsuyoshi Ueda
Folate is an important vitamin mainly ingested from vegetables, and folate deficiency causes various health problems. Recently, several studies demonstrated folate biofortification in plants or food crops by metabolic engineering through genetic modifications. However, genetically modified foods may have adverse effects, and their production and sales are under strict regulation. Here, we developed a new approach to achieve folate biofortification in spinach (Spinacia oleracea) without genetic modification...
May 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28548713/co-culture-systems-based-strategies-for-articular-cartilage-tissue-engineering
#8
REVIEW
Yu Zhang, Shuyun Liu, Weimin Guo, Mingjie Wang, Chunxiang Hao, Shuang Gao, Xueliang Zhang, Li Xu, Mingxue Chen, Li Penghao, Peng Jiang, Shibi Lu, Quanyi Guo
Cartilage engineering facilitates repair and regeneration of damaged cartilage using engineered tissue that restores the functional properties of the impaired joint. The seed cells used most frequently in tissue engineering, are chondrocytes and mesenchymal stem cells. Seed cells activity plays a key role in the regeneration of functional cartilage tissue. However, seed cells undergo undesirable changes after in vitro processing procedures, such as degeneration of cartilage cells and induced hypertrophy of mesenchymal stem cells, which hinder cartilage tissue engineering...
May 26, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28548675/inducible-bcl-2-gene-rna-interference-mediated-by-aptamer-integrated-hdv-ribozyme-switch
#9
Yuanyuan Zhang, Jine Wang, Hui Cheng, Na Sun, Min Liu, Zhengyan Wu, Renjun Pei
The regulation of RNA interference (RNAi) could be a powerful method for the study of temporal and dose dependent effects of gene expression. In this study, we designed the hepatitis delta virus (HDV) ribozyme with an embedded theophylline aptamer as the sensor domain and the pri-miRNA of endogenous gene Bcl-2 as the effector domain to engineer an RNAi-regulatory device in MCF-7 cells. The system allowed us to control gene expression by adding theophylline into the culture media in a dose dependent fashion...
May 26, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28548630/tenogenic-differentiation-of-human-embryonic-stem-cells
#10
Tina P Dale, Shazia Mazher, William R Webb, Jing Zhou, Nicola Maffulli, G-Q Chen, Alicia J El Haj, Nicholas R Forsyth
Tendon healing is complex to manage because of the limited regeneration capacity of tendon tissue; stem cell-based tissue engineering approaches may provide alternative healing strategies. We sought to determine whether human embryonic stem cells (hESC) could be induced to differentiate into tendon-like cells by the addition of exogenous bone morphogenetic protein (BMP)12 (growth differentiation factor(GDF)7) and BMP13 (GDF6). hESC (SHEF-1) were maintained with or without BMP12/13 supplementation, or supplemented with BMP12/13 and the SMAD signalling cascade blocking agent, dorsomorphin...
May 26, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28548628/endothelial-progenitor-cells-for-the-vascularization-of-engineered-tissues
#11
Erica Brown Peters
Self-assembled microvasculature from cocultures of endothelial cells and stromal cells have significantly advanced efforts to vascularize engineered tissues by enhancing perfusion rates <i>in vivo</i> and producing investigative platforms for microvascular morphogenesis <i>in vitro</i>. However, in order to clinically translate prevascularized constructs, the issue of endothelial cell source must be resolved. Endothelial progenitor cells (EPCs) can be noninvasively supplied from the recipient through adult peripheral and umbilical cord blood, as well as derived from induced pluripotent stem cells, alleviating antigenicity issues...
May 26, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28548584/the-sex-disparity-in-risky-driving-a-survey-of-colombian-young-drivers
#12
Oscar Oviedo-Trespalacios, Bridie Scott-Parker
OBJECTIVE: The overrepresentation of young drivers in poor road safety outcomes has long been recognised as a global road safety issue. In addition, the overrepresentation of males in crash statistics has also been recognised as a pervasive young driver problem. Whilst progress in road safety evidenced as a stabilisation and/or reduction in poor road safety outcomes has been made in developed nations, less-developed nations contribute the greatest road safety trauma, and developing nations such as Colombia continue to experience increasing trends in fatality rates...
May 26, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28548581/modeling-crash-injury-severity-by-road-feature-to-improve-safety
#13
Praveena Penmetsa, Srinivas S Pulugurtha
Structured Abstract Objective: The objective of this research is two-fold; a) to model and identify critical road features (or locations) based on crash injury severity and compare it with crash frequency, and, b) to model and identify drivers who are more likely to contribute to crashes by road feature. METHOD: Crash data from 2011 to 2013 was obtained from the Highway Safety Information System (HSIS) for the state of North Carolina. Twenty-three different road features were considered, analyzed and compared with each other as well as no road feature...
May 26, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28548569/thyroxine-increases-collagen-type-ii-expression-and-accumulation-in-scaffold-free-tissue-engineered-articular-cartilage
#14
G Adam Whitney, Thomas J Kean, Russell J Fernandes, Stephen D Waldman, M Yat Tse, Stephen C Pang, Joseph M Mansour, James E Dennis
Low collagen accumulation in the extra-cellular matrix is a pressing problem in cartilage tissue engineering, leading to a low collagen-to-glycosaminoglycan (GAG) ratio and poor mechanical properties in neo-cartilage. Soluble factors have been shown to increase collagen content, but may result in a more pronounced increase in GAG content. Thyroid hormones have been reported to stimulate collagen and GAG production, but reported outcomes, including which specific collagen types are affected, are variable throughout the literature...
May 26, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28548559/the-yucatan-minipig-tmj-disc-structure-function-relationships-support-its-suitability-for-human-comparative-studies
#15
Natalia Vapniarsky, Ashkan Aryaei, Boaz Arzi, Jerry C Hu, David C Hatcher, Kyriacos A Athanasiou
Frequent involvement of the disc in temporomandibular joint (TMJ) disorders warrants attempts to tissue engineer TMJ disc replacements. Physiologically, a great degree of similarity is seen between humans and farm pigs (FPs), but the pig's rapid growth confers a significant challenge for in vivo experiments. Minipigs have a slower growth rate and are smaller than FPs, but minipig TMJ discs have yet to be fully characterized. The objective of this study was to determine the suitability of the minipig for TMJ studies by extensive structural and functional characterization...
May 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28548530/strain-induced-gauge-field-and-landau-levels-in-acoustic-structures
#16
Zhaoju Yang, Fei Gao, Yahui Yang, Baile Zhang
The emerging field of topological acoustics that explores novel gauge-field-related phenomena for sound has drawn attention in recent years. However, previous approaches constructing a synthetic gauge field for sound predominantly relied on a periodic system, being unable to form a uniform effective magnetic field, thus lacking access to some typical magnetic-induced quantum phenomena such as Landau energy quantization. Here we introduce strain engineering, previously developed in graphene electronics and later transferred to photonics, into a two-dimensional acoustic structure in order to form a uniform effective magnetic field for airborne acoustic wave propagation...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548503/modeling-evaporation-and-particle-assembly-in-colloidal-droplets
#17
Mingfei Zhao, Xin Yong
Evaporation-induced assembly of nanoparticles in a drying droplet is of great importance in many engineering applications, including printing, coating and thin film processing. The investigation of particle dynamics in evaporating droplets can provide fundamental hydrodynamic insight for revealing the processing-structure relationship in the particle self-organization induced by solvent evaporation. We develop a free-energy-based multiphase lattice Boltzmann method coupled with Brownian dynamics to simulate evaporating colloidal droplets on solid substrates with specified wetting properties...
May 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28548488/a-fluorescent-split-aptamer-for-visualizing-rna-rna-assembly-in-vivo
#18
Khalid K Alam, Kwaku D Tawiah, Matthew F Lichte, David Porciani, Donald H Burke
RNA-RNA assembly governs key biological processes and is a powerful tool for engineering synthetic genetic circuits. Characterizing RNA assembly in living cells often involves monitoring fluorescent reporter proteins, which are at best indirect measures of underlying RNA-RNA hybridization events and are subject to additional temporal and load constraints associated with translation and activation of reporter proteins. In contrast, RNA aptamers that sequester small molecule dyes and activate their fluorescence are increasingly utilized in genetically encoded strategies to report on RNA-level events...
May 26, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28548484/controlled-release-of-naringin-in-metal-organic-frameworks-mofs-loaded-mineralized-collagen-coating-to-simultaneously-enhance-osseointegration-and-antibacterial-activity
#19
Mengfei Yu, Dongqi You, Junjun Zhuang, Suya Lin, Lingqing Dong, Shengtao Weng, Bin Zhang, Kui Cheng, Wen-Jian Weng, Huiming Wang
Two important goals in orthopedic implants research are to promote osseointegration and prevent infection. However, much of previous efforts have been paid on the design of coatings either to enhance osseointegration while ignoring antibacterial activity or vice versa, to prevent infection while ignoring bone integration. Here, we designed a multifunctional mineralized collagen coating on titanium with the aid of metal-organic frameworks (MOFs) nanocrystals to control the release of naringin, a Chinese herbal medicine which could promote osseointegration and prevent bacterial infection...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28548289/effects-of-food-borne-nanomaterials-on-gastrointestinal-tissues-and-microbiota
#20
REVIEW
Hans Bouwmeester, Meike van der Zande, Mark A Jepson
Ingestion of engineered nanomaterials is inevitable due to their addition to food and prevalence in food packaging and domestic products such as toothpaste and sun cream. In the absence of robust dosimetry and particokinetic data, it is currently challenging to accurately assess the potential toxicity of food-borne nanomaterials. Herein, we review current understanding of gastrointestinal uptake mechanisms, consider some data on the potential for toxicity of the most commonly encountered classes of food-borne nanomaterials (including TiO2 , SiO2, ZnO, and Ag nanoparticles), and discuss the potential impact of the luminal environment on nanoparticle properties and toxicity...
May 26, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
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